Supplementary. presence of the. (c) mrna expression. Error. in naive or
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1 Figure 1. (a) Naive CD4 + T cells were activated in the presence of the indicated cytokines for 3 days. Enpp2 mrna expression was measured by qrt-pcrhr, infected with (b, c) Naive CD4 + T cells were activated under T H 0 condition for 36 retrovirus expressing JunB or control shrna, and then incubated for another 3 days in the presence of IL-6, IL-1 and IL-23. Enpp2 (b) and Il23r (c) mrna expression was measured by qrt-pcr. (a-c) (n=3). Asterisks indicate significant differences (p<0.05) by unpaired two-tailed Student s t test. (d) Immunoblot analysis of JunB in naive or activated CD4 + Results were normalized to Hprt mrna expression. Error bars indicate s.d. T cells under TH0, H T H 17( ) and T H 17( (23) conditions for 60 hr. (a-d) Data represent two independent experiments.
2 Figure 2. (a) Schematic illustration showing the strategy to generate floxed JunB mice. (b) Naive CD4 + T cells from wild-type C57BL/6, Cd4 Cre JunB fl/ /fl and control JunB fl/fl mice were activated under T H 17( ) conditions for 3 days, and JunB was detected by immunoblot analysis. Dataa represent two independent experiments.
3 Figure 3. Flow cytometry data showing cells expressing CD4 and CD8 in lymphocytes (a) or CD62L and CD44 in CD4 + cells (b) in LNs and spleens of Cd4 Cre JunB fl/fl and control JunB fl/fl mice. NS, not significant differences (p>0.05) by unpaired two-tailed Student s t test. Data represent two independent experiments.
4 Figure 4. (a, b) Naive CD4 + T cells from Cd4 Cre JunB fl/fl and control JunB fl/fl mice were activated under T H 17( ) )- or T H 17( )-polarizingg conditions for 3 days, and expression of IL-17A and GM-CSF (a) or ROR t and Foxp3 (b) was analyzed by flow cytometry. (c) Naive CD4 T cells were activated under T H H17( ) or T H 17(23) conditions for 3 days. Il6r mrna expression was measured by qrt-pcr. Dataa were normalized to Hprt mrna. Error bars indicate s.d. (n=3). Asterisks indicate significant differences (P<0.05) by unpaired two-tailed Student s t test. NS, not significant. (d) Naive CD4 + T cells weree activated under T H 17( ) )- or itreg-polarizing conditions for 3 days. Foxp3 expression was analyzed by flow cytometry. (e) Naive CD4 + T cells were activated in the presence of TGF- 3 and IL-6 (T H 17( 3)) for 3 days, and expression of ROR t and Foxp3 was analyzed by flow cytometry. (f) Naive CD4 + T cells were activated under T H H17( ) conditions for 3 days, and expression of IL-17A and Foxp3 was analyzed by flow cytometry. The right graph showss mean of fluorescence intensity (MFI) of IL-17A expression. Error bars indicate s.d. (n=3). Asterisks indicate significant differences (P<0.05) by unpaired two-tailed Student s t test. (g) Naive CD4 + T cells were activated in the presence of TGF- and IL-6 with or without IL-23 for 3 days. Expression of IL-17A and IFN- was detected by flow cytometry. (a-g) Data represent two independent experiments. (h) Gating strategy for flow cytometry analysis in Fig. 2e. Dead cells were stained with Zombie dye.
5 Figure 5. (a) Effect of JunB deficiency on expression of all common T H 17 genes thatt are significantly upregulated in both T H 17( ( ) and T H 17(23) compared to T H H0. Heat map data show fold changes of expression in Cd4 Cre JunB fl/fl (KO) versus control (WT) cells as in Fig. 3c. (b, c) Naive CD4 + T from Cd4 Cre JunB fl/fl and control mice were activated under T H 17( ), T H 17(23) (b) or T H 17( 3) (c) conditions for 84 hr, and mrna expression of indicated genes was analyzed by qrt-pcr. (d) Naive CD4 + T cells were activated under T H 17( )-polarizing conditions for 60 hr, and IL-17-highh and IL-17-low populations were sorted by FACS and used for microarray analysis. Heat map data show fold changes of expression in Cd4 Cre JunB fl/fll (KO) versus control (WT) cells for common T H 17 genes and genes categorized as transcription factors. Only genes that showed a significant change in Cd4 Cre JunB fl/fl cells compared to controls were shown. (e) qrt-pcr analysis of IL-17-high and IL-17-low populations in JunB-deficient or control cells activated under T H 17( ) conditions. (f) Flow cytometry analysis of cells activated under T H 17(23) conditions for 3 days. T-bet and ROR t expression was detected. (b, c, e) Error bars indicate s.d. (n=3). Asterisks indicate significant differences (p<0.05) by unpaired two-tailed Student s t test. ns, not significant. (a-e) Data represent two independent experiments.
6 Figure 6. Naive CD4 + T cells from wild-type mice were activated under T H 17( )- or T H 17(23)-polarizing conditions for 60 hr and subjected to ChIP-seq analysis using JunB, BATF, and IRF4 antibodies. Il23r (a), Tbx21 (b), Il10 (c), Prdm1 (d), Cd5l (e) loci are shown. Schematics representations at the top of panels indicate exons (black boxes), and introns (solid lines). Data represent two independent experiments.
7 Figure 7. (a) Absolute numbers of CD3 + CD4 + cells expressing IL-17A, IFN-, ROR t, and Foxp3 were determined using flow cytometry dataa in Fig. 5. (b) Flow cytometryy analysis of cells expressing IL-17A and IFN- in CD4 + cells in LNs and spleens of Cd4 Cre JunB fl/fl and control JunB fl/fl l mice. Data represent at least two independent experiments. (c) Absolute numbers of CD3 + CD4 + cells expressing IL-17A and IFN- were determinedd using flow cytometryy data in Fig. 6c. (d) Absolute numbers of CD3 + CD4 + cells expressing IL-17A or IFN- in spleenss of EAE-induced mice. (e) Frequencies of CD4 + EYFP + cells in LNs and CNS of Il17a Cre R26R eyfp JunB fl/fl or control Il17a Cre R26R EYF JunB +/+ mice on day 14 after EAEE induction (as in Fig. 6d). (a-e) Error bars indicate s.d. n=3 (a, b, d), n=4 (c, e). Asterisks indicate significant differences (p<0.05) by unpaired two-tailed Student s t test. NS, not significant. Data represent two independent experiments.
8 Figure 8. (a, b) Colitis was induced in Rag1-deficient mice by transferring CD4 + CD45RB hi CD25 - cells from Cd4 Cre JunB fl l/fl or control JunB fl/fl mice. Frequencies of CD4 + T cells expressing IL-17A and IFN- in colonic LP (a) and LNs (b) were analyzed. Data represent at least two independent experiments s. (c) Mice (n=5) weree injected with anti-cd3 antibody three times at 0, 48, and 96 hr. At 4 hr after the final injection, cells were isolated from LP of duodenums and used for flow cytometry analysis (gated on CD4 + and TCR + ). Asterisks indicate significant differences (p< <0.05) by unpaired two-tailed Student s t test. NS, not significant. Dataa represent two independent experiments.
9 Figure 9. Naive CD4+ T cells from Cd4 Cre JunB fl/fl or control Ju unb fl/fl mice were activated under T H 17( ) conditions in the presence of TGF- receptor kinase inhibitor (SB43152), JNK inhibitor (SP600125), MEK inhibitor (PD98059), p38 inhibitor (SB203580), PI3 kinase inhibitor (LY294002), or ROCK inhibitor (Y27632), SMAD3 inhibitor (SIS3) or DMSO alone for 60 hr. Rorc mrna levels were measured by qrt-pcr. Results weree normalized to Hprt mrna expression. Error bars indicate s.d. (n=3). Asterisks indicate significant differences (p<0.05) compared to DMSO-treated control samples by unpaired two-tailed Student s t test. NS, not significant. Dataa represent two independent experiments.
10 Figure 10. Gating strategies for flow cytometry analyses of T cells differentiated in vitro related to Fig. 2 (a), T cells isolated from LNs, spleen and gut related to Figs. 5 and 6 (b), and duodenum T cells related to Supplementary Figure 8 (c).
11 Figure 11. Original data of immunoblots. (a) Immunoblot data corresponding to Fig. 1a. (b) Immunoblot data corresponding to Fig. 1c. (c) Immunoblot data corresponding to Fig. 1d. (d) Immunoblot data corresponding to Fig. 1e. (e) Immunoblot data corresponding to Fig. 2b.
12 Table 1. Primers used in qrt-pcr.and ChIP-PCR
Reviewers' comments: Reviewer #1 (Remarks to the Author):
Reviewers' comments: Reviewer #1 (Remarks to the Author): In this manuscript, Hasan et al analyse the transcriptional program used by pathogenic Th17 cells raised in the presence of IL-23 as compared to
Supplementary Figure 1. Efficiency of Mll4 deletion and its effect on T cell populations in the periphery. Nature Immunology: doi: /ni.
Supplementary Figure 1 Efficiency of Mll4 deletion and its effect on T cell populations in the periphery. Expression of Mll4 floxed alleles (16-19) in naive CD4 + T cells isolated from lymph nodes and
% of live splenocytes. STAT5 deletion. (open shapes) % ROSA + % floxed
Supp. Figure 1. a 14 1 1 8 6 spleen cells (x1 6 ) 16 % of live splenocytes 5 4 3 1 % of live splenocytes 8 6 4 b 1 1 c % of CD11c + splenocytes (closed shapes) 8 6 4 8 6 4 % ROSA + (open shapes) % floxed
Supplemental Materials
Supplemental Materials Programmed death one homolog maintains the pool size of regulatory T cells by promoting their differentiation and stability Qi Wang 1, Jianwei He 1, Dallas B. Flies 2, Liqun Luo
and follicular helper T cells is Egr2-dependent. (a) Diagrammatic representation of the
Supplementary Figure 1. LAG3 + Treg-mediated regulation of germinal center B cells and follicular helper T cells is Egr2-dependent. (a) Diagrammatic representation of the experimental protocol for the
SUPPLEMENTARY INFORMATION
doi: 1.138/nature89 IFN- (ng ml ) 5 4 3 1 Splenocytes NS IFN- (ng ml ) 6 4 Lymph node cells NS Nfkbiz / Nfkbiz / Nfkbiz / Nfkbiz / IL- (ng ml ) 3 1 Splenocytes IL- (ng ml ) 1 8 6 4 *** ** Lymph node cells
The encephalitogenicity of TH17 cells is dependent on IL-1- and IL-23- induced production of the cytokine GM-CSF
CORRECTION NOTICE Nat.Immunol. 12, 568 575 (2011) The encephalitogenicity of TH17 cells is dependent on IL-1- and IL-23- induced production of the cytokine GM-CSF Mohamed El-Behi, Bogoljub Ciric, Hong
Supplementary Figure 1. mrna expression of chitinase and chitinase-like protein in splenic immune cells. Each splenic immune cell population was
Supplementary Figure 1. mrna expression of chitinase and chitinase-like protein in splenic immune cells. Each splenic immune cell population was sorted by FACS. Surface markers for sorting were CD11c +
Supplementary Figure S1. PTPN2 levels are not altered in proliferating CD8+ T cells. Lymph node (LN) CD8+ T cells from C57BL/6 mice were stained with
Supplementary Figure S1. PTPN2 levels are not altered in proliferating CD8+ T cells. Lymph node (LN) CD8+ T cells from C57BL/6 mice were stained with CFSE and stimulated with plate-bound α-cd3ε (10µg/ml)
Supplemental Figure 1. Signature gene expression in in vitro differentiated Th0, Th1, Th2, Th17 and Treg cells. (A) Naïve CD4 + T cells were cultured
Supplemental Figure 1. Signature gene expression in in vitro differentiated Th0, Th1, Th2, Th17 and Treg cells. (A) Naïve CD4 + T cells were cultured under Th0, Th1, Th2, Th17, and Treg conditions. mrna
Supplementary Figure 1 IL-27 IL
Tim-3 Supplementary Figure 1 Tc0 49.5 0.6 Tc1 63.5 0.84 Un 49.8 0.16 35.5 0.16 10 4 61.2 5.53 10 3 64.5 5.66 10 2 10 1 10 0 31 2.22 10 0 10 1 10 2 10 3 10 4 IL-10 28.2 1.69 IL-27 Supplementary Figure 1.
SUPPLEMENTARY MATERIAL
SUPPLEMENTARY MATERIAL IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the T H 17 cell it reg cell balance Rajatava Basu 1,5, Sarah K.
Nature Immunology: doi: /ni Supplementary Figure 1. Gene expression profile of CD4 + T cells and CTL responses in Bcl6-deficient mice.
Supplementary Figure 1 Gene expression profile of CD4 + T cells and CTL responses in Bcl6-deficient mice. (a) Gene expression profile in the resting CD4 + T cells were analyzed by an Affymetrix microarray
ECM1 controls T H 2 cell egress from lymph nodes through re-expression of S1P 1
ZH, Li et al, page 1 ECM1 controls T H 2 cell egress from lymph nodes through re-expression of S1P 1 Zhenhu Li 1,4,Yuan Zhang 1,4, Zhiduo Liu 1, Xiaodong Wu 1, Yuhan Zheng 1, Zhiyun Tao 1, Kairui Mao 1,
Nature Immunology: doi: /ni Supplementary Figure 1. Id2 and Id3 define polyclonal T H 1 and T FH cell subsets.
Supplementary Figure 1 Id2 and Id3 define polyclonal T H 1 and T FH cell subsets. Id2 YFP/+ (a) or Id3 GFP/+ (b) mice were analyzed 7 days after LCMV infection. T H 1 (SLAM + CXCR5 or CXCR5 PD-1 ), T FH
Supplementary Figure 1. IL-12 serum levels and frequency of subsets in FL patients. (A) IL-12
1 Supplementary Data Figure legends Supplementary Figure 1. IL-12 serum levels and frequency of subsets in FL patients. (A) IL-12 serum levels measured by multiplex ELISA (Luminex) in FL patients before
SUPPLEMENTARY FIGURES
SUPPLEMENTARY FIGURES Supplementary Figure 1: Chemokine receptor expression profiles of CCR6 + and CCR6 - CD4 + IL-17A +/ex and Treg cells. Quantitative PCR analysis of chemokine receptor transcript abundance
Supplementary Figure 1. Characterization of basophils after reconstitution of SCID mice
Supplementary figure legends Supplementary Figure 1. Characterization of after reconstitution of SCID mice with CD4 + CD62L + T cells. (A-C) SCID mice (n = 6 / group) were reconstituted with 2 x 1 6 CD4
Supplemental Figure 1
Supplemental Figure 1 1a 1c PD-1 MFI fold change 6 5 4 3 2 1 IL-1α IL-2 IL-4 IL-6 IL-1 IL-12 IL-13 IL-15 IL-17 IL-18 IL-21 IL-23 IFN-α Mut Human PD-1 promoter SBE-D 5 -GTCTG- -1.2kb SBE-P -CAGAC- -1.kb
Supplementary Fig. 1 No relative growth advantage of Foxp3 negative cells.
Supplementary Fig. 1 Supplementary Figure S1: No relative growth advantage of Foxp3 negative cells. itreg were induced from WT (A) or FIR (B) CD4 + T cells. FIR itregs were then removed from the TCR signal
Tbk1-TKO! DN cells (%)! 15! 10!
a! T Cells! TKO! B Cells! TKO! b! CD4! 8.9 85.2 3.4 2.88 CD8! Tbk1-TKO! 1.1 84.8 2.51 2.54 c! DN cells (%)! 4 3 2 1 DP cells (%)! 9 8 7 6 CD4 + SP cells (%)! 5 4 3 2 1 5 TKO! TKO! TKO! TKO! 15 1 5 CD8
<10. IL-1β IL-6 TNF + _ TGF-β + IL-23
3 ns 25 ns 2 IL-17 (pg/ml) 15 1 ns ns 5 IL-1β IL-6 TNF
Supplementary Figure 1. Normal T lymphocyte populations in Dapk -/- mice. (a) Normal thymic development in Dapk -/- mice. Thymocytes from WT and Dapk
Supplementary Figure 1. Normal T lymphocyte populations in Dapk -/- mice. (a) Normal thymic development in Dapk -/- mice. Thymocytes from WT and Dapk -/- mice were stained for expression of CD4 and CD8.
Transcription factor Foxp3 and its protein partners form a complex regulatory network
Supplementary figures Resource Paper Transcription factor Foxp3 and its protein partners form a complex regulatory network Dipayan Rudra 1, Paul deroos 1, Ashutosh Chaudhry 1, Rachel Niec 1, Aaron Arvey
Supplementary Materials for
www.sciencetranslationalmedicine.org/cgi/content/full/8/352/352ra110/dc1 Supplementary Materials for Spatially selective depletion of tumor-associated regulatory T cells with near-infrared photoimmunotherapy
Supplementary Figure 1
d f a IL7 b IL GATA RORγt h HDM IL IL7 PBS Ilra R7 PBS HDM Ilra R7 HDM Foxp Foxp Ilra R7 HDM HDM Ilra R7 HDM. 9..79. CD + FOXP + T reg cell CD + FOXP T conv cell PBS Ilra R7 PBS HDM Ilra R7 HDM CD + FOXP
Supplementary Figure 1. ETBF activate Stat3 in B6 and Min mice colons
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Nature Medicine doi: /nm.3957
Supplementary Fig. 1. p38 alternative activation, IL-21 expression, and T helper cell transcription factors in PDAC tissue. (a) Tissue microarrays of pancreatic tissue from 192 patients with pancreatic
SUPPLEMENT Supplementary Figure 1: (A) (B)
SUPPLEMENT Supplementary Figure 1: CD4 + naïve effector T cells (CD4 effector) were labeled with CFSE, stimulated with α-cd2/cd3/cd28 coated beads (at 2 beads/cell) and cultured alone or cocultured with
Supplemental Materials for. Effects of sphingosine-1-phosphate receptor 1 phosphorylation in response to. FTY720 during neuroinflammation
Supplemental Materials for Effects of sphingosine-1-phosphate receptor 1 phosphorylation in response to FTY7 during neuroinflammation This file includes: Supplemental Table 1. EAE clinical parameters of
Nature Immunology: doi: /ni Supplementary Figure 1. Characteristics of SEs in T reg and T conv cells.
Supplementary Figure 1 Characteristics of SEs in T reg and T conv cells. (a) Patterns of indicated transcription factor-binding at SEs and surrounding regions in T reg and T conv cells. Average normalized
for six pairs of mice. (b) Representative FACS analysis of absolute number of T cells (CD4 + and
SUPPLEMENTARY DATA Supplementary Figure 1: Peripheral lymphoid organs of SMAR1 -/- mice have an effector memory phenotype. (a) Lymphocytes collected from MLNs and Peyer s patches (PPs) of WT and SMAR1
NMED-A65251A. Supplementary Figures.
NMED-A65251A Supplementary Figures. Sup. Fig. 1. ILC3 cells are the main source of in obese mice a. We gated on T cells (upper panels) or T cells (lower panels), and examined production. b. CD45 + - IL-13
Cell isolation. Spleen and lymph nodes (axillary, inguinal) were removed from mice
Supplementary Methods: Cell isolation. Spleen and lymph nodes (axillary, inguinal) were removed from mice and gently meshed in DMEM containing 10% FBS to prepare for single cell suspensions. CD4 + CD25
Supplementary Table 1. Oligonucleotide primers used in quantitative and qualitative PCRs of this study.
Supplementary Table 1. Oligonucleotide primers used in quantitative and qualitative PCRs of this study. Primer Sequence Qualitative PCR primers Universal 16S rdna forward 5 - GACGCTGAGGCATGAGAGCA-3 Universal
Supplementary Fig. 1 p38 MAPK negatively regulates DC differentiation. (a) Western blot analysis of p38 isoform expression in BM cells, immature DCs
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Supplementary information. The proton-sensing G protein-coupled receptor T-cell death-associated gene 8
1 Supplementary information 2 3 The proton-sensing G protein-coupled receptor T-cell death-associated gene 8 4 (TDAG8) shows cardioprotective effects against myocardial infarction 5 Akiomi Nagasaka 1+,
Supplemental Figure 1. Activated splenocytes upregulate Serpina3g and Serpina3f expression.
Relative Serpin expression 25 2 15 1 5 Serpina3f 1 2 3 4 5 6 8 6 4 2 Serpina3g 1 2 3 4 5 6 C57BL/6 DBA/2 Supplemental Figure 1. Activated splenocytes upregulate Serpina3g and Serpina3f expression. Splenocytes
Supporting Information Table of Contents
Supporting Information Table of Contents Supporting Information Figure 1 Page 2 Supporting Information Figure 2 Page 4 Supporting Information Figure 3 Page 5 Supporting Information Figure 4 Page 6 Supporting
Therapeutic effect of baicalin on experimental autoimmune encephalomyelitis. is mediated by SOCS3 regulatory pathway
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Supplementary Information
Supplementary Information Supplementary Figure 1! a! b! Nfatc1!! Nfatc1"! P1! P2! pa1! pa2! ex1! ex2! exons 3-9! ex1! ex11!!" #" Nfatc1A!!" Nfatc1B! #"!" Nfatc1C! #" DN1! DN2! DN1!!A! #A!!B! #B!!C! #C!!A!
Akt and mtor pathways differentially regulate the development of natural and inducible. T H 17 cells
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D CD8 T cell number (x10 6 )
IFNγ Supplemental Figure 1. CD T cell number (x1 6 ) 18 15 1 9 6 3 CD CD T cells CD6L C CD5 CD T cells CD6L D CD8 T cell number (x1 6 ) 1 8 6 E CD CD8 T cells CD6L F Log(1)CFU/g Feces 1 8 6 p
Supplementary Figure 1 Cytokine receptors on developing thymocytes that can potentially signal Runx3d expression.
Supplementary Figure 1 Cytokine receptors on developing thymocytes that can potentially signal Runx3d expression. (a) Characterization of c-independent SP8 cells. Stainings for maturation markers (top)
Bezzi et al., Supplementary Figure 1 *** Nature Medicine: doi: /nm Pten pc-/- ;Zbtb7a pc-/- Pten pc-/- ;Pml pc-/- Pten pc-/- ;Trp53 pc-/-
Gr-1 Gr-1 Gr-1 Bezzi et al., Supplementary Figure 1 a Gr1-CD11b 3 months Spleen T cells 3 months Spleen B cells 3 months Spleen Macrophages 3 months Spleen 15 4 8 6 c CD11b+/Gr1+ cells [%] 1 5 b T cells
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Supplementary Figure 1 Loss of Mst1 and Mst2 increases susceptibility to bacterial sepsis. (a) H&E staining of colon and kidney sections from wild type and Mst1 -/- Mst2 fl/fl Vav-Cre mice. Scale bar,
Nature Immunology: doi: /ni Supplementary Figure 1. Transcriptional program of the TE and MP CD8 + T cell subsets.
Supplementary Figure 1 Transcriptional program of the TE and MP CD8 + T cell subsets. (a) Comparison of gene expression of TE and MP CD8 + T cell subsets by microarray. Genes that are 1.5-fold upregulated
Supplemental Table I.
Supplemental Table I Male / Mean ± SEM n Mean ± SEM n Body weight, g 29.2±0.4 17 29.7±0.5 17 Total cholesterol, mg/dl 534.0±30.8 17 561.6±26.1 17 HDL-cholesterol, mg/dl 9.6±0.8 17 10.1±0.7 17 Triglycerides,
SUPPLEMENTARY INFORMATION
Supplemental Figure 1. Furin is efficiently deleted in CD4 + and CD8 + T cells. a, Western blot for furin and actin proteins in CD4cre-fur f/f and fur f/f Th1 cells. Wild-type and furin-deficient CD4 +
Nature Medicine: doi: /nm.3922
Title: Glucocorticoid-induced tumor necrosis factor receptor-related protein co-stimulation facilitates tumor regression by inducing IL-9-producing helper T cells Authors: Il-Kyu Kim, Byung-Seok Kim, Choong-Hyun
Supplemental Figure 1. Protein L
Supplemental Figure 1 Protein L m19delta T m1928z T Suppl. Fig 1. Expression of CAR: B6-derived T cells were transduced with m19delta (left) and m1928z (right) to generate CAR T cells and transduction
Supporting Information
Supporting Information Stegbauer et al. 10.1073/pnas.0903602106 SI Methods Analysis of Plasma Renin Activity (PRA) and ACE Activity. PRA and serum ACE activity levels were determined by RIA (RENCTK, DiaSorin;
Nature Immunology: doi: /ni Supplementary Figure 1. Cytokine pattern in skin in response to urushiol.
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Supplementary Figure 1. Generation of knockin mice expressing L-selectinN138G. (a) Schematics of the Sellg allele (top), the targeting vector, the
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Supplementary Information Epigenetic modulation of inflammation and synaptic plasticity promotes resilience against stress in mice
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Supplementary Figure 1: TSLP receptor skin expression in dcssc. A: Healthy control (HC) skin with TSLP receptor expression in brown (10x
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Supplementary Figures
Supplementary Figures Supplementary Figure 1. NKT ligand-loaded tumour antigen-presenting B cell- and monocyte-based vaccine induces NKT, NK and CD8 T cell responses. (A) The cytokine profiles of liver
Supplementary Figure 1: Expression of NFAT proteins in Nfat2-deleted B cells (a+b) Protein expression of NFAT2 (a) and NFAT1 (b) in isolated splenic
Supplementary Figure 1: Expression of NFAT proteins in Nfat2-deleted B cells (a+b) Protein expression of NFAT2 (a) and NFAT1 (b) in isolated splenic B cells from WT Nfat2 +/+, TCL1 Nfat2 +/+ and TCL1 Nfat2
Supplementary Figure 1. Repression of hepcidin expression in the liver of mice treated with
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Supplementary Figure 1. Deletion of Smad3 prevents B16F10 melanoma invasion and metastasis in a mouse s.c. tumor model.
A B16F1 s.c. Lung LN Distant lymph nodes Colon B B16F1 s.c. Supplementary Figure 1. Deletion of Smad3 prevents B16F1 melanoma invasion and metastasis in a mouse s.c. tumor model. Highly invasive growth
Supplementary Information
Supplementary Information mediates STAT3 activation at retromer-positive structures to promote colitis and colitis-associated carcinogenesis Zhang et al. a b d e g h Rel. Luc. Act. Rel. mrna Rel. mrna
Supplementary Figure 1. Antibiotic partially rescues mice from sepsis. (ab) BALB/c mice under CLP were treated with antibiotic or PBS.
1 Supplementary Figure 1. Antibiotic partially rescues mice from sepsis. (ab) BALB/c mice under CLP were treated with antibiotic or PBS. (a) Survival curves. WT Sham (n=5), WT CLP or WT CLP antibiotic
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Krishnamoorthy et al., c d e ND ND Supplementary Figure 1 RSV-induces inflammation even in the asence of allergen. Tolerized pups were either infected with RSV or not. The mice were sacrificed a week following
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W/T Itgam -/- F4/80 CD115. F4/80 hi CD115 + F4/80 + CD115 +
F4/8 % in the peritoneal lavage 6 4 2 p=.15 n.s p=.76 CD115 F4/8 hi CD115 + F4/8 + CD115 + F4/8 hi CD115 + F4/8 + CD115 + MHCII MHCII Supplementary Figure S1. CD11b deficiency affects the cellular responses
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Title of file for HTML: Supplementary Information Description: Supplementary Figures and Supplementary Table Title of file for HTML: Peer Review File Description: Innate Scavenger Receptor-A regulates
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Supplementary information CD4 T cells are required for both development and maintenance of disease in a new model of reversible colitis
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Supplementary Figure 1 CD4 + T cells from PKC-θ null mice are defective in NF-κB activation during T cell receptor signaling. CD4 + T cells were
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Reviewers' comments: Reviewer #1 Expert in EAE and IL-17a (Remarks to the Author):
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Supplementary table I. Real-time primers used in the study. The fold change was obtained by
Supplementary table I. Real-time primers used in the study. The fold change was obtained by normalizing the gene expression number to those of HPRT, then comparing the samples to untreated or naive mice.
of whole cell cultures in U-bottomed wells of a 96-well plate are shown. 2
Supplementary online material Supplementary figure legends Supplementary Figure 1 Exposure to T reg cells causes loss of T resp cells in co-cultures. T resp cells were stimulated with CD3+CD28 alone or
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