attomol HLA-B*27-Realtime LT 2 Assay for the detection of the human HLA-B*27-locus using LightCycler (Do not use for tissue typing!
|
|
- Buck Russell
- 5 years ago
- Views:
Transcription
1 attomol HLA-B*27-Realtime LT 2 Assay for the detection of the human HLA-B*27-locus using LightCycler (Do not use for tissue typing!) For in vitro diagnostic use only! 50 determinations Order number: 95 C. Introduction The Human Leukocyte-Antigens (HLA) are glycoproteins of the major histocompatibility complex (MHC), which are expressed on nearly all nucleated cells. These antigens define the immunological individuality (differentiation between endogenic, i.e. internally generated and exogenic, i.e. foreign ). The HLA-B antigens belong together with HLA-A and HLA-C to the MHC class I molecules and their genes are located on the short arm of chromosome 6 (Thomas, 2000, Labor und Diagnose, 5. erweiterte Auflage, TH-Books Verlagsgesellschaft mbh, S ). There exist different forms of HLA-B antigens, the so-called determinants (e.g. HLA-B7, -B8, -B5,...). One of these determinants - HLA-B27 - was found in 973 to be closely related to certain rheumatic diseases, especially to Spondylitis ancylosans (SPA, Morbus Bechterew) (Brewerton et al., 973, Lancet : ). Carriers of HLA-B27 bear an 87-fold increase in the risk to suffer from SPA as carriers of other determinants. The disease manifests very often between the 5th and 30th year of life and in 90 % of all cases is found in men. HLA-B27 occurs in about 7 % of the Western Europe population. Patients with SPA carry HLA-B27 in % of all cases (Isselbacher et al., 995, Harrisons Innere Medizin, 3. Auflage, Blackwell-Wissenschaftsverlag, S ). However, the pathogenesis is not completely understood (Taurog, J Rheumatol 200, 37(2):2606-6). Until now, HLA-B27 was detected serologically. Recently, this method is increasingly replaced by molecular biological techniques, because molecular tests work without living cells and are cheaper and easier to perform. 2. Important Remarks Keep the kit at the indicated storage temperature. This kit should only be used for in vitro diagnostic applications. Only use the latest provided manual for test realization. Do not use this kit after the expiration date. To carry out the assay take care for an appropriate laboratory equipment and well-trained staff. Wear gloves during all steps of the test application. Patient s samples should be considered as a potentially infectious agent and should therefore be handled according to the current law. Store reagents for the PCR apart from patient DNA samples and amplification products. 3. Materials included Order Number 326 Reagent Volumes Storage Temperature Oligomix HLA-B*27 LT 2 (blue cap) tube with 00 µl primer/probe mix, ready to use -20 C until first use, after first use store tubes dark at 4 C* * Freezing of oligomix after first use is recommended only if you do not plan to use it during the next 4 weeks.
2 2 4. Additional Reagents and Materials not included Patient s DNA samples (use genomic DNA dissolved in aqua dest. or TE-buffer) Control template (HLA-B*27 positive DNA) Micropipettes (volume range µl) and sterile filter tips From the kit LightCycler Faststart-DNA-Master HypProbe from Roche (catalog number: or ): mix vial no. a (red cap) with vial no. b (colorless cap) according to instructions of Roche, resulting in 0x ready to use mix (contains Taq polymerase, nucleotides, MgCl 2 ) hereinafter called Roche mix PCR-water LightCycler -capillaries (20 µl, for LightCycler 2; order no ) or suitable PCR tubes for LightCycler 480 LightCycler 2.0 or 480 with appropriate control and evaluation software Color compensation file generated with the attomol color compensation kit 2 (order no. 97) 5. Test Principle The kit described here can be used to determine whether a patient carries the HLA-B27 allele or not. More detailed HLA-B27 subtyping or typing of other HLA-B determinants is not possible by using this test kit. This test only can evaluate the risk for rheumatic diseases (refer to paragraph ) but it is not allowed to use this test for tissue typing. After isolating DNA from patient s samples, the HLA-B*27-locus and a reference gene used as an internal standard become amplified parallel within one PCR reaction. Detection of amplification products takes place by hybridisation of the specific mutation-covering LoopTag* ) probe. The LoopTag probe hybridises to the elongated primer forming a primer-probe loop. The formation of a loop enables FRET between the fluorescence donor (D) and the fluorescence acceptor (A) as shown in fig.. Dehybridisation of LoopTag probe takes places during melting curve analysis and thereby confirms the amplification of the HLA-B*27-locus through specific melting temperatures (see subsection 6 Manual, subheading Protocol, sub item Evaluation ). The amplification of the internal standard indicates the proper process of PCR especially in the case of HLA-negative samples. A clinical diagnosis should not be concluded from results of one diagnostic method alone. The doctor has to consider all clinical and laboratory results available. 3 A primerelongation FRET A D 3 Fig. : LoopTag detection principle probe hybridisation * ) LoopTag is patented by Attomol (patent-no. PCT/EP2008/05752)
3 3 6. Manual In advance to the first usage of this test kit a color compensation file has to be generated with attomol color compensation kit 2 (order no. 97). It is necessary to include the following set of control PCR reactions in addition to patient s samples:. Negative control (PCR-blank) 2. Positive control (HLA-B*27 positive sample) The positive control may be ordered separately from the manufacturer (order number 06). Patient s samples that were genotyped correctly by the customer can also be used as positive control. Protocol: DNA-preparation from patient s blood according to standard procedures; resulting DNA samples (app. 20 ng/µl) can be used without dilution. Attention: If you have any problems (poor amplification, not evaluable melting curves) at first repeat the assay with diluted DNA samples (e.g. :0). Thaw tubes with oligomix, shake and centrifuge the tubes to pull down condensed water. Storage remark: After taking off the needed amount of oligomix you can store the rest of it in the refrigerator (4 C, dark). Freezing of oligomix is recommended only if you do not plan to use it during the next days. Before you take it again please centrifuge and mix it well. Generally avoid repeated freezing-thawing-cycles of the oligomix! Preparation of the PCR mix (8 µl PCR mix und 2 µl sample DNA are required for one PCR reaction) According to the number of samples pipette PCR water, Roche-Mix, and oligomix in an Eppendorf tube for the preparation of PCR mix (see following table). Mix it well after preparation. number of samples PCR water Roche-Mix oligomix number of samples PCR water Roche-Mix oligomix Transfer of reagents to LightCycler -capillaries or -tubes Negative control: Positive control: Patient s sample: 8 µl PCR mix 8 µl PCR mix 8 µl PCR mix + 2 µl PCR-H 2 O + 2 µl DNA (HLA-B*27 positive) + 2 µl DNA Seal the capillaries/tubes and centrifuge. PCR incubation of the samples is performed in the LightCycler according to the program listed in appendix. Evaluation of the samples is done by analysis of amplification signals and melting peaks according to the following tables. A second human gene is amplified in all samples serving as an internal standard (IS). For HLA- B*27-negativ or not clearly identifiable samples it has to be verified if an amplification signal of the internal standard is existent. If for a sample neither a melting curve signal nor an amplification curve signal can be detected an inhibition of the PCR can be concluded. In this case the relevant sample has to be tested again (dilute the sample or repeat the DNA extraction).
4 4 Evaluation criteria: HLA-B*27 positive samples: LC 2.0 LC 480 HLA-B*27 amplification signal at 70 nm with clearly sigmoid or exponential growth, c t -value < 40, peak maximum of melting curve at 65 C ± 2 C amplification signal at 660, 670 or 705 nm with clearly exponential or sigmoid growth, c t-value < 40, peak maximum of melting curve at 65 C ± 2 C HLA-B*27 negative samples: LC 2.0 LC 480 HLA-B*27 no amplification signal at 70 nm, straight curve, slight, continuous slope possible, without melting curve peak or melting curve peak outside of the tolerance region at 65 C ± 2 C (e.g. in the case of other HLA-types) no amplification signal at 660, 670 or 705 nm, straight curve, slight, continuous slope possible, without melting curve peak or melting curve peak outside of the tolerance region at 65 C ± 2 C (e.g. in the case of other HLA-types) choose wavelengths according to the applied set of filters Internal standard amplification signal at 560 nm with sigmoid or exponential growth, c t-value < 36, can drop out in the case of a strongly HLA-B*27-positive sample amplification signal at 568 or 580 nm with sigmoid or exponential growth, c t-value < 36, can drop out in the case of a strongly HLA-B*27-positive sample Internal standard amplification signal at 560 nm with sigmoid or exponential growth, c t-value < 36 amplification signal at 568 or 580 nm with sigmoidal or exponential growth, c t-value < 36 Fig. 2: Examples for the evaluation of a positive HLA-B*27 sample (red curve), a negative sample (blue curve), and a blank (negative control, black curve) The example data were obtained by using LightCycler 480. A: amplification curves for HLA-B*27 B: melting curves for HLA-B*27 C: amplification curves for the internal standard In the case of problems during the test application do not hesitate to contact the manufacturer or the distributor.
5 5 Annex The wavelengths necessary for excitation and detection can be found in the following table: excitation detection for HLA-B*27 detection for internal standard (IS) LC 2.0 automatic 70 nm (F6) 560 nm (F2) LC 480 HLA-B*27: 483 or 498 nm 660, 670 or 705 nm IS: 523 or 533 nm 568 or 580 nm choose wavelengths according to the applied set of filters To perform the PCR and melting curve analysis use the programs in the following tables. Program for LC 2.0 Program for LC 480 Program: Activation; Type: None; Cycles: Program: Activation; Analysis : None; Cycles: Temperature Hold Time Slope Target Hold Ramp Rate Target ( C) (sec) ( C/sec) ( C) (s) ( C/s) none 95 none Program: Amplification; Type: Quantification; Cycles: 45 Program: Amplification; Analysis : Quantification; Cycles: 45 Temperature Target ( C) Hold Time (sec) Slope ( C/sec) Target ( C) Hold (s) Ramp Rate ( C/s) none 95 none single 62 single none 72 none Program: melting curve; Type: Melt. Curves; Cycles: Program: melting curve; Analysis : Melt. Curves; Cycles: Temperature Target ( C) Hold Time (sec) Slope ( C/sec) Target ( C) Hold (s) Ramp Rate ( C/s) none 95 none none 50 none none 40 none continuous 80 continuous Program: Cooling; Type: None; Cycles: Program: Cooling; Analysis : None; Cycles: Temperature Hold Time Slope Target Hold Ramp Rate Target ( C) (sec) ( C/sec) ( C) (s) ( C/s) none 40 none Annex 2 Overview about symbols used for labeling of tubes and kit. explanation of symbols h manufacturer l batch number v expiry date 2 t 6 store between +2 C and +6 C o -20 store at a maximum of -20 C b catalogue number i in vitro diagnostic medical devices pn content sufficient for <n> determinations g consult instructions for use
For in vitro Veterinary Diagnostics only. Kylt Rotavirus A. Real-Time RT-PCR Detection.
For in vitro Veterinary Diagnostics only. Kylt Rotavirus A Real-Time RT-PCR Detection www.kylt.eu DIRECTION FOR USE Kylt Rotavirus A Real-Time RT-PCR Detection A. General Kylt Rotavirus A products are
More informationRIDA GENE HLA-B27 PY0205
RIDA GENE HLA-B27 PY0205 R-Biopharm AG, An der neuen Bergstraße 17, 64297 Darmstadt, Germany Phone: +49 (0) 61 51 81 02-0 / Fax: +49 (0) 61 51 81 02-20 1. Intended use For in vitro diagnostic use. With
More informationRealLine Mycoplasma genitalium Str-Format
Instructions for use ASSAY KIT FOR THE QUALITATIVE DETECTION OF MYCOPLASMA GENITALIUM DNA BY REAL-TIME PCR METHOD In vitro Diagnostics () VBD4396 96 Tests valid from December 2018 Rev06_1218_EN Page 1
More informationaltona RealStar Instructions for Use RealStar CMV PCR Kit /2017 EN DIAGNOSTICS
altona DIAGNOSTICS Instructions for Use RealStar CMV PCR Kit 1.2 08/2017 EN RealStar RealStar CMV PCR Kit 1.2 For research use only! (RUO) 021202 INS-021200-EN-S01 48 08 2017 altona Diagnostics GmbH Mörkenstr.
More informationAmoyDx TM BRAF V600E Mutation Detection Kit
AmoyDx TM BRAF V600E Mutation Detection Kit Detection of V600E mutation in the BRAF oncogene Instructions For Use Instructions Version: B3.1 Date of Revision: April 2012 Store at -20±2 o C 1/5 Background
More informationRayBio DPP4 Inhibitor Screening Kit
RayBio DPP4 Inhibitor Screening Kit User Manual Version 1.0 Mar 25, 2013 RayBio DPP4 Inhibitor Screening (Cat#: 68SR-DPP4-S100) RayBiotech, Inc. We Provide You With Excellent Support And Service Tel:(Toll
More informationMolecularMD. One-Step qrt-pcr BCR-ABL Kit. Product Description and User Manual. For Quantitative RT-PCR Analysis of BCR-ABL. Contact Us.
Contact Us If you have any questions for or comments about MolecularMD, please feel free to contact us. Email Customer Service CustomerService@MolecularMD.com Technical Support TechSupport@MolecularMD.com
More informationvirellatbe real time RT-PCR Kit LC
Instruction for Use virellatbe real time RT-PCR Kit LC For the in-vitro detection of TBE Virus RNA in clinical specimens and in ticks. G01065-32 G01065-96 32 96 gerbion gmbh & Co. KG Remsstr. 1 70806 Kornwestheim
More informationPneumocystis Carinii Real Time PCR Kit. For In Vitro Diagnostic Use Only User Manual
Revision No.: ZJ0003 Issue Date: Aug 7 th, 2008 Pneumocystis Carinii Real Time PCR Kit Cat. No.: QD-0082-02 For use with ABI Prism 7000/7300/7500/7900; Smart CyclerII; icycler iq 4/iQ 5; Rotor Gene 2000/3000;
More informationRayBio Maltose and Glucose Assay Kit
RayBio Maltose and Glucose Assay Kit User Manual Version 1.0 May 8, 2014 RayBio Maltose and Glucose Assay Kit Protocol (Cat#:68-MalGlu-S100) RayBiotech, Inc. We Provide You With Excellent Support And Service
More informationEpiQuik Circulating Acetyl Histone H3K18 ELISA Kit (Colorimetric)
EpiQuik Circulating Acetyl Histone H3K18 ELISA Kit (Colorimetric) Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE Uses: The EpiQuik Circulating Acetyl Histone H3K18 ELISA Kit (Colorimetric)
More informationSERUM GLUCAGON KITS PROTOCOL
SERUM GLUCAGON KITS PROTOCOL Part # 62SGLPEB & 62SGLPEF Test size#: 5 x 200 tests (62SGLPEB), 200 tests (62SGLPEF) - assay volume: 20 µl Revision: 02-Jan.2018 Store at: -60 C or below (62SGLPEB); -60 C
More informationMicrosart Calibration Reagent
Instructions for Use Microsart Calibration Reagent Prod. No. SMB95-2021 Mycoplasma arginini Prod. No. SMB95-2022 Mycoplasma orale Prod. No. SMB95-2023 Mycoplasma gallisepticum Prod. No. SMB95-2024 Mycoplasma
More informationRealLine HCV Qualitative Str-Format
Instructions for use REAL TIME PCR DETECTION KIT FOR THE HEPATITIS C VIRUS RNA (HCV) Research Use Only (RUO) (Str-format) VBD0795 96 Tests valid from: October 2018 Rev05_1018_EN Page 1 of 8 Explanation
More informationLeukemia BCR-ABL Fusion Gene Real Time RT-PCR Kit
Revision No.: ZJ0003 Issue Date: Aug 7 th, 2008 Leukemia BCR-ABL Fusion Gene Real Time RT-PCR Kit Cat. No.: TR-0126-02 For use with ABI Prism 7000/7300/7500/7900(96 well); Smart Cycler II; icycler iq 4/iQ
More informationINSULIN MOUSE SERUM KITS
INSULIN MOUSE SERUM KITS PROTOCOL Part # 62IN3PEF & 62IN3PEB Test size#: 200 tests (62IN3PEF), 5 x 200 tests (62IN3PEB) - assay volume: 20 µl Revision: 03-Jan.2018 Store at: 2-8 C (62IN3PEF); 2-8 C (62IN3PEB)
More informationGalactose and Lactose Assay Kit
Galactose and Lactose Assay Kit Catalog Number KA0842 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
More information1. Intended Use New Influenza A virus real time RT-PCR Panel is used for the detection of universal influenza A virus, universal swine Influenza A vir
New Influenza A Virus Real Time RT-PCR Kit User Manual LT028310RRFY - 1 - 1. Intended Use New Influenza A virus real time RT-PCR Panel is used for the detection of universal influenza A virus, universal
More informationCoenzyme A Assay Kit. Catalog Number KA assays Version: 02. Intended for research use only.
Coenzyme A Assay Kit Catalog Number KA0809 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials Supplied...
More informationRayBio Acid Phosphatase Activity Colorimetric Assay. Kit. User Manual Version 1.0 May 5, RayBiotech, Inc. Kit Protocol. (Cat#: 68CL-AcPh-S500)
RayBio Acid Phosphatase Activity Colorimetric Assay Kit User Manual Version 1.0 May 5, 2014 RayBio Acid Phosphatase Activity Colorimetric Assay (Cat#: 68CL-AcPh-S500) RayBiotech, Inc. We Provide You With
More informationRealLine HIV qualitative Str-Format
Instructions for Use REAL TIME PCR DETECTION KIT FOR HUMAN IMMUNODEFICIENCY VIRUS RNA Research Use Only (RUO) RealLine HIV Qualitative (Str-format) VBD0196 96 Tests valid from July 2016 Rev01072016_EN
More informationRealLine HBV / HCV / HIV Str-Format
Instructions for use ASSAY KIT FOR THE QUALITATIVE AND DIFFERENTIAL DETECTION OF HEPATITIS B VIRUS DNA, HEPATITIS C VIRUS RNA, AND HUMAN IMMUNODEFICIENCY VIRUS TYPES 1 AND 2 RNA USING THE PCR/RT-PCR METHOD
More informationMouse serum Insulin 200 tests
Headquarters & Europe Office Cisbio Bioassays Phone: +33 (0)4 66 79 67 05 Fax: +33 (0)4 66 79 19 20 bioassays@cisbio.com cisbio_dd_pi_62in3pef-2µl USA Office Cisbio US, Inc. Phone: +1 888 963 4567 Fax:
More informationInstructions for use. TSH rat ELISA. Please use only the valid version of the Instructions for Use provided with the kit AR E-8600
Instructions for use TSH rat ELISA AR E-8600 TSH rat ELISA 1. INTRODUCTION 1.1 Intended use The TSH rat ELISA is an enzyme immunoassay for the quantitative measurement of TSH in rat serum. For research
More informationInstructions for Use. RealStar Influenza Screen & Type RT-PCR Kit /2017 EN
Instructions for Use RealStar Influenza Screen & Type RT-PCR Kit 4.0 05/2017 EN RealStar Influenza Screen & Type RT-PCR Kit 4.0 For research use only! (RUO) 164003 INS-164000-EN-S01 96 05 2017 altona
More informationEPIGENTEK. EpiQuik HDAC2 Assay Kit (Colorimetric) Base Catalog # P-4006 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE
EpiQuik HDAC2 Assay Kit (Colorimetric) Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik HDAC2 Assay Kit is very suitable for measuring HDAC2 levels from various fresh tissues and
More informationCisbio Bioassays MAP-Tau assay is only intended for quantitative measurement of microtubule-associated protein tau (MAP-Tau) using HTRF technology.
MAP-TAU KITS PROTOCOL Part # 63ADK008PEG & 63ADK008PEH Test size#: 500 tests (63ADK008PEG), 10,000 tests (63ADK008PEH) - assay volume: 20 µl Revision: 02-Jan.2018 Store at: -20 C or below (63ADK008PEG);
More informationFor the rapid, sensitive and accurate measurement of Triglyceride in various samples.
ab65336 Triglyceride Quantification Kit Instructions for Use For the rapid, sensitive and accurate measurement of Triglyceride in various samples. This product is for research use only and is not intended
More informationHuman Rotavirus A. genesig Standard Kit. Non structural protein 5 (NSP5) 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Human Rotavirus A Non structural protein 5 (NSP5) genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Human Rotavirus A Rotavirus is a genus
More informationLumino Firefly Luciferase Assay
G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Lumino Firefly Luciferase Assay (Cat. # 786 1267, 786 1268) think proteins! think G-Biosciences
More informationEPIGENTEK. EpiQuik HDAC Activity/Inhibition Assay Kit(Colorimetric) Base Catalog # P-4002 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE
EpiQuik HDAC Activity/Inhibition Assay Kit(Colorimetric) Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik HDAC Activity/Inhibition Assay Kit (Colorimetric) is very suitable for
More informationInstructions for Use. IA2 Antibody ELISA. Enzyme Immuno Assay for the Quantitative Determination of Antibodies against IA-2 in Serum.
Instructions for Use IA2 Antibody ELISA Enzyme Immuno Assay for the Quantitative Determination of Antibodies against IA-2 in Serum I V D REF EA114/96 12 x 8 2 8 C DLD Gesellschaft für Diagnostika und medizinische
More informationBranched Chain Amino Acid (Leu, Ile, Val) Assay Kit
Branched Chain Amino Acid (Leu, Ile, Val) Assay Kit Catalog Number KA0824 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General
More informationHuman Rotavirus B. Non structural protein 5 (NSP5) 150 tests. Quantification of Human Rotavirus B genomes Advanced kit handbook HB10.01.
PCR Max Ltd TM qpcr test Human Rotavirus B Non structural protein 5 (NSP5) 150 tests For general laboratory and research use only 1 Introduction to Human Rotavirus B Rotavirus is a genus of double-stranded
More informationFor the rapid, sensitive and accurate measurement of Glucose in various samples
ab65333 Glucose Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Glucose in various samples This product is for research use only and is not intended for diagnostic use.
More informationFor the rapid, sensitive and accurate measurement of Sucrose levels in various samples
ab83387 Sucrose Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Sucrose levels in various samples This product is for research use only and is not intended for diagnostic
More informationRayBio Phenylalanine Fluorometric Assay Kit
RayBio Phenylalanine Fluorometric Assay Kit User Manual Version 1.0 June 25, 2014 RayBio Phenylalanine Fluorometric (Cat#: 68-Phenyl-S100) RayBiotech, Inc. We Provide You With Excellent Support And Service
More informationHuman TSH ELISA Kit. User Manual
Human TSH ELISA Kit User Manual Catalog number: GTX15585 GeneTex Table of Contents A. Product Description... 2 B. Kit Components... 3 C. Additional Required Materials (not included)... 3 D. Reagent Preparation...
More informationAnnexin V-FITC Apoptosis Detection Kit with SYTOX
ab14086 Annexin V-FITC Apoptosis Detection Kit with SYTOX Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in living cells. This product is for research use only and
More informationXTRAKT FFPE Kit / Manual
XTRAKT FFPE Kit / Manual For manual extraction of RNA, mirna and/or DNA from Formalin Fixed Paraffin Embedded (FFPE) tissue samples Catalog No. # XTK2.0-96 For research use only. Not intended for diagnostic
More informationRealLine HCV quantitative Str-Format
Instructions for use QUANTITATIVE KIT FOR REAL TIME PCR DETECTION FOR RNA OF HEPATITIS C VIRUS Attention! Please read the information about quantification process carefully! Research Use Only (RUO) (Str
More informationHuman Rotavirus A. genesig Advanced Kit. Non structural protein 5 (NSP5) 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Human Rotavirus A Non structural protein 5 (NSP5) genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Human Rotavirus A Rotavirus is a genus
More informationab HIV-1 Protease Inhibitor Screening Kit (Fluorometric)
Version 1 Last updated 2 March 2017 ab211106 HIV-1 Protease Inhibitor Screening Kit (Fluorometric) For the rapid, sensitive and accurate screening of potential HIV-1 Protease inhibitors. This product is
More informationInstructions for Use. RealStar Influenza S&T RT-PCR Kit /2017 EN
Instructions for Use RealStar Influenza S&T RT-PCR Kit 3.0 01/2017 EN RealStar Influenza S&T RT-PCR Kit 3.0 For research use only! (RUO) 163003 INS-163000-EN-S02 96 01 2017 altona Diagnostics GmbH Mörkenstr.
More informationInternal Validation Guide of Y-STR Systems for Forensic Laboratories Printed in USA. 11/12 Part# GE713
REFERENCE MANUAL Internal Validation Guide of Y-STR Systems for Forensic Laboratories 11/12 Internal Validation Guide of Y-STR Systems for Forensic Laboratories PLEASE DISCARD PREVIOUS VERSIONS. All technical
More informationEpiQuik HDAC Activity/Inhibition Assay Kit (Fluorometric)
EpiQuik HDAC Activity/Inhibition Assay Kit (Fluorometric) Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik HDAC Activity/Inhibition Assay Kit (Fluorometric) is very suitable for
More informationInfluenza A IgG ELISA
Influenza A IgG ELISA For the qualitative determination of IgG-class antibodies against Influenza A virus in human serum or plasma (citrate, heparin). For Research Use Only. Not For Use In Diagnostic Procedures.
More informationHuman influenza A virus subtype (H3) genesig Easy Kit for use on the genesig q reaction. Primerdesign Ltd
TM Primerdesign Ltd Human influenza A virus subtype (H3) genesig Easy Kit for use on the genesig q16 50 reaction For general laboratory and research use only 1 genesig Easy: at a glance guide For each
More informationHuman Influenza A (Swine Flu) Rapid test
Human Influenza A (Swine Flu) Rapid test Cat.No: DTSXY-Z9 Lot. No. (See product label) Size 20T Intended use The Influenza A (Swine Flu) test is a rapid chromatographic immunoassay for the qualitative
More informationHuman influenza A virus subtype (H1)
PCRmax Ltd TM qpcr test Human influenza A virus subtype (H1) Haemoglutinin H1 gene 150 tests For general laboratory and research use only 1 Introduction to Human influenza A virus subtype (H1) Influenza,
More informationHIV-1 Viral Load Real Time (RG)
-1 Viral Load Real Time (RG) Real Time RT-PCR type 1 RNA quantification assay MSP Reg. pending Valdense 3616. 11700. Montevideo. Uruguay. phone (598) 2 336 83 01. Fax (598) 2 336 71 60. Info@atgen.com.uy
More informationTotal Histone H3 Acetylation Detection Fast Kit (Colorimetric)
Total Histone H3 Acetylation Detection Fast Kit (Colorimetric) Catalog Number KA1538 48 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use...
More informationGlucose and Sucrose Assay Kit
ab65334 Glucose and Sucrose Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Glucose and Sucrose levels in various samples This product is for research use only and is
More informationKit Components Product # EP42720 (24 preps) MDx 2X PCR Master Mix 350 µl Cryptococcus neoformans Primer Mix 70 µl Cryptococcus neoformans Positive
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cryptococcus neoformans End-Point PCR Kit Product# EP42720 Product
More informationAmantadine ELISA Test Kit
Amantadine ELISA Test Kit Catalog No. LSY-10052 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Amantadine. The coupling antigen is pre-coated on the micro-well
More informationAnnexin V-Cy3 Apoptosis Detection Reagent
ab14143 Annexin V-Cy3 Apoptosis Detection Reagent Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in various samples This product is for research use only and is not
More informationInstructions for use. TSH rat ELISA. Please use only the valid version of the Instructions for Use provided with the kit AR E-8600
Instructions for use TSH rat ELISA AR E8600 TSH rat ELISA INTRODUCTION INTENDED USE The TSH rat ELISA is an enzyme immunoassay for the quantitative measurement of TSH in rat serum. For research use only.
More informationLipase Detection Kit II (Colorimetric)
ab102525 Lipase Detection Kit II (Colorimetric) Instructions for Use For the rapid, sensitive and accurate measurement of Lipase activity in various samples. This product is for research use only and is
More informationProduct # Kit Components
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Pneumocystis jirovecii PCR Kit Product # 42820 Product Insert Background Information
More informationGlobal Histone H3 Acetylation Assay Kit
Global Histone H3 Acetylation Assay Kit Catalog Number KA0633 96 assays Version: 06 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle
More informationRapid-VIDITEST Swine Flu
Rapid-VIDITEST Swine Flu One Step Influenza type A Antigen Card test. Instruction manual Producer: VIDIA spol. s r.o., Nad Safinou II 365, 252 50 Vestec, Czech Republic, Tel.: +420 261 090 565, www.vidia.cz
More informationIntroduction to Cladosporium_spp
Techne qpcr test Cladosporium_spp 150 tests For general laboratory and research use only 1 Introduction to Cladosporium_spp Cladosporium is a genus of slow growing fungi, colonies are mostly dark green
More informationEpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric)
EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric) Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik Total Histone H3 Acetylation Detection Fast Kit (Colorimetric)
More informationRapid-VIDITEST. Influenza A+B
Rapid-VIDITEST Influenza A+B (One step Influenza A+B blister Test for the detection of Influenza type A and type B from nasal swabs, nasal wash or nasal aspirate specimens). Instruction manual Producer:
More informationPD1/PD-L1 BINDING ASSAY KITS
PD1/PD-L1 BINDING ASSAY KITS PROTOCOL Part # 64ICP01PEG & 64ICP01PEH Test size: 500 tests (64ICP01PEG), 10,000 tests (64ICP01PEH) - assay volume: 20 µl Revision: 02 (July 2017) Store at: -60 C or below
More informationRealLine HIV quantitative Str-Format
Instructions for use DETECTION AND QUANTIFICATION OF THE HUMAN IMMUNODEFICIENCY VIRUS RNA BY REAL TIME PCR Research Use Only (RUO) Attention! Please read the information about quantification process carefully!
More informationHBV Real-TM Quant Handbook
HBV Real-TM Quant Handbook Real Time Kit for the Quantitative detection of Hepatitis B Virus in human plasma for use with SmartCycler (Cepheid), RotorGene 3000/6000 (Corbett Research), iq icycler and iq5
More informationNorgen s HIV proviral DNA PCR Kit was developed and validated to be used with the following PCR instruments: Qiagen Rotor-Gene Q BioRad icycler
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com HIV Proviral DNA PCR Kit Product # 33840 Product Insert Background Information
More informationFinTest IgG4 Screen 20 ELISA KIT
FinTest IgG4 Screen 20 ELISA KIT Cat. No.:DEIA6196 Pkg.Size:96T Intended use Enzyme immunoassay (microtiter strips) for the detection and the quantitative determination of IgG4 antibodies against 20 Food
More informationPhosphate buffered saline (PBS) for washing the cells TE buffer (nuclease-free) ph 7.5 for use with the PrimePCR Reverse Transcription Control Assay
Catalog # Description 172-5080 SingleShot Cell Lysis Kit, 100 x 50 µl reactions 172-5081 SingleShot Cell Lysis Kit, 500 x 50 µl reactions For research purposes only. Introduction The SingleShot Cell Lysis
More informationSwine H1N1 Influenza Human Pandemic Strain. genesig Easy Kit 2 Target Gene Kit for use on the genesig q reaction.
TM Primerdesign Ltd Swine H1N1 Influenza Human Pandemic Strain genesig Easy Kit 2 Target Gene Kit for use on the genesig q16 50 reaction For general laboratory and research use only 1 genesig Easy: at
More informationRayBio Cathepsin D Activity Assay Kit
RayBio Cathepsin D Activity Assay Kit User Manual Version 1.0 October 1, 2014 RayBio Cathepsin D Activity Assay (Cat#: 68AT-CathD-S100) RayBiotech, Inc. We Provide You With Excellent Support And Service
More informationHuman influenza A virus subtype (H3)
PCRmax Ltd TM qpcr test Human influenza A virus subtype (H3) Haemoglutinin H3 gene 150 tests For general laboratory and research use only 1 Introduction to Human influenza A virus subtype (H3) Influenza,
More informationHuman Rotavirus C. genesig Advanced Kit. DNA testing. Everything... Everyone... Everywhere... Non structural protein 5 (NSP5) 150 tests
TM Primerdesign Ltd TM Primerdesign Ltd Human Rotavirus C Non structural protein 5 (NSP5) genesig Advanced Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory and research
More informationCladosporium_spp genesig Advanced Kit
TM Primerdesign Ltd Cladosporium_spp genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Cladosporium_spp Cladosporium is a genus of slow growing fungi, colonies
More informationCladosporium_spp genesig Standard Kit
TM Primerdesign Ltd Cladosporium_spp genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Cladosporium_spp Cladosporium is a genus of slow growing fungi, colonies
More informationHDAC Activity/Inhibition Assay Kit (Fluorometric)
HDAC Activity/Inhibition Assay Kit (Fluorometric) Catalog Number KA0627 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background...
More informationHBeAg and HBeAg Ab ELISA Kit
HBeAg and HBeAg Ab ELISA Kit Catalog Number KA0290 96 assays Version: 17 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Principle of the Assay... 3
More informationSensoLyte 520 HIV-1 Protease Assay Kit *Fluorimetric*
SensoLyte 520 HIV-1 Protease Assay Kit *Fluorimetric* Catalog # 71147 Kit Size 100 assays (96-well) or 500 assays (384-well) Convenient Format: Complete kit including all the assay components. Optimized
More informationAvian Influenza A H5N8
TM Primerdesign Ltd Avian Influenza A H5N8 Hemagglutinin (HA) gene & Neuraminidase (NA) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Avian Influenza
More informationCathepsin K Activity Assay Kit
Cathepsin K Activity Assay Kit Catalog Number KA0769 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
More informationAmpliX HBV Quantitative
Instructions for use REAL TIME PCR DETECTION AND QUANTITATION KIT OF HEPATITIS B VIRUS DNA Research Use Only (RUO) (Lyo-format) VBD0595 96 rcs valid from May 2013 Explanation of symbols used in labeling
More informationHuman Cytomegalovirus IgM ELISA Kit
Human Cytomegalovirus IgM Catalog No: IRAPKT2012 ELISA Kit Lot No: SAMPLE INTENDED USE The CMV IgM ELISA is intended for use in the detection of IgM antibodies to Cytomegalovirus (CMV) infection in human
More informationEPIGENTEK. EpiQuik Global Acetyl Histone H3K27 Quantification Kit (Colorimetric) Base Catalog # P-4059 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE
EpiQuik Global Acetyl Histone H3K27 Quantification Kit (Colorimetric) Base Catalog # P-4059 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik Global Acetyl Histone H3K27 Quantification Kit (Colorimetric)
More informationEPIGENTEK. EpiQuik Global Histone H3 Acetylation Assay Kit. Base Catalog # P-4008 PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE
EpiQuik Global Histone H3 Acetylation Assay Kit Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE The EpiQuik Global Histone H3 Acetylation Assay Kit is suitable for specifically measuring global
More informationHuman Cytomegalovirus Virus (CMV) IgG ELISA Kit
Human Cytomegalovirus Virus Catalog No: IRAPKT1410 (CMV) IgG ELISA Kit Lot No: SAMPLE INTENDED USE The CMV IgG ELISA is intended for use in evaluating a patient s serologic status to cytomegalovirus (CMV)
More informationAscorbic Acid Assay Kit
ab65346 Ascorbic Acid Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Ascorbic Acid in various samples This product is for research use only and is not intended for
More informationRapid-VIDITEST. Rota-Adeno Blister. One Step Rotavirus and Adenovirus Antigen Blister test. Instruction manual
Rapid-VIDITEST Rota-Adeno Blister One Step Rotavirus and Adenovirus Antigen Blister test. Instruction manual INTENDED USE: The Rapid-VIDITEST Rota-Adeno Blister test is a rapid chromatographic immunoassay
More informationMouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit
Mouse HBsAg(Hepatitis B Virus Surface Antigen) ELISA Kit Catalogue No.: EM0002 Size: 96T Reactivity: Mouse Application: This immunoassay kit allows for the qualitative determination of HBsAg in Mouse serum
More informationGlucose Assay Kit. Catalog Number KA assays Version: 07. Intended for research use only.
Glucose Assay Kit Catalog Number KA0831 100 assays Version: 07 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials Supplied...
More informationMyBioSource.com. Instructions for use. Histamine Release
Instructions for use Histamine Release Supplementary kit for the determination of the release of histamine from heparinized whole blood (this kit has to be used in combination with the Histamine ELISA,
More informationACTG Laboratory Technologist Committee Revised Version 2.0 ACTG Lab Man Coulter HIV-1 p24 ELISA May 21, 2004
Coulter HIV p24 1. PRINCIPLE The Human Immunodeficiency Virus Type 1 (HIV-1) is recognized as the etiologic agent of acquired immunodeficiency syndrome (AIDS). The virus is transmitted by sexual contact,
More informationIV2-113E Use by. Invitron Glargine ELISA Kit REF LOT IVD. Definitions. English. For in-vitro diagnostic use. Instructions for use.
Definitions Instructions for use REF Catalogue number IV2-113E Use by English Invitron Glargine ELISA Kit For in-vitro diagnostic use Σ 96 LOT IVD Lot/Batch Code Storage temperature limitations In vitro
More informationNorgen s HIV Proviral DNA PCR Kit was developed and validated to be used with the following PCR instruments: Qiagen Rotor-Gene Q BioRad T1000 Cycler
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com HIV Proviral DNA PCR Kit Product# 33840 Product Insert Intended
More informationFor the rapid, sensitive and accurate measurement of Maltose levels in various samples.
ab83388 Maltose Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of Maltose levels in various samples. This product is for research use only and is not intended for diagnostic
More informationRat Hemoglobin A1c (HbA1c) Kit Instructions
V.3 Crystal Chem Rat Hemoglobin A1c (HbA1c) Kit Instructions For the quantitative determination of hemoglobin A1c (HbA1c) in rat whole blood Catalog #80300 96 Assays For research use only. Not for use
More informationProtease Assay. (Cat. # ) think proteins! think G-Biosciences
389PR 01 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Protease Assay (Cat. # 786 028) think proteins! think G-Biosciences www.gbiosciences.com
More informationHuman Albumin ELISA KIT
Human Albumin ELISA KIT Cat. No.:DEIA9947 Pkg.Size:96T Intended use The Human Albumin ELISA KIT is intended for the quantitative determination of Albumin in urine and stool. General Description Albumin
More information