Secretory Lysosomes and Diseases
|
|
- Garry Barrett
- 5 years ago
- Views:
Transcription
1 ISSN CN / Q http / / cjbmb bjmu edu cn Chinese Journal of Biochemistry and Molecular Biology ~ * Rab27a ATP Rab27a Q25 Secretory Lysosomes and Diseases WANG Jun-Wei 1 ZHOU Yi-Jiang 2 1 ZHU Ke-Qing 3 * 1 Department of Neurobiology Zhejiang University School of Medicine Hangzhou 2 Zhejiang University School of Medicine Hangzhou China 3 Department of Pathology Zhejiang University School of Medicine Hangzhou China China Abstract The classical lysosomes contain a variety of hydrolytic enzymes and lipase to decompose proteins and membrane structures and considered as the cellular terminus digestive organelle The secretory lysosomes are dicovered in certain cell types with the functions of both intracellular digestion and regulatory exocytosis Rab27a plays a central role in the regulation of the exocytosis of lysosomal proteins The exocytosis-related gene mutations particularly in the regulatory proteins can cause a variety of immune deficiency phenotypes The ATP released from the astrocytes lysosomes can be used as the transmission signal between neurons and glial cells The secretory lysosomes also get involved in the invasion and metastasis of cancer cells Key words secretory lysosomes exocytosis Rab27a T B ph 3 40 T No Y T * Tel zhukeqing@ zju edu cn Received August Accepted November Supported by Natural Science Foundation of Zhejiang Province No Y * Corresponding author Tel zhukeqing@ zju edu cn
2 2 131 natural killer cell NK Rab1-Rab43 Rab3 Rab3A / B / C / D tumor necrosis factor TNF Rab27 Rab27A / B PC12 Rab3a Rab27a PC12 B 12 Rab27a Rab Rab27a 1 3 ATP Rab27a 13 4 Munc13-4 Munc13-4 JFC NK T Rab27a 3 Slp synaptotagminlike protein Slp / Rab cytotoxic T lymphocyte CTL Slac2 Slp C2 / Noc2 Munc Slp / Rab N C Rab27a Rab27a peripheral central supramolecular activation complex psmac F- 2 microtubule organizing centre 11 JFC1 Munc13-4 Rab27a Rab27a Slac2 MTOC Rab27a Va VIIa IL-1b ATP Slp Ca Rab27a Rab27a 6 Rab27a Va VIIa Munc SNARE 9 10 Fig 1 2 Rab27a ATP T ATP ATP 3 G Rab27a N- Nethylmaleimide-sensitive factor attachment protein Ca 2 + kiss and run 18 SNARE 18 ATP receptors SNARE ATP Rab- vesicular nucleotide transporter VNUT GTP 60
3 Fig 1 Secretory lysosome exocytosis A Stage 1 on recognition of a target cell by the natural killer NK cell a lytic immunological synapse forms at the point of contact with the target cell and the actin cytoskeleton is reorganized forming a ring of F-actin around the psmac B Stage 2 the MTOC and secretory lysosomes become polarized towards the lytic synapse It is likely that the secretory lysosomes move along microtubules to the lytic synapse but this has not been confirmed in NK cells C Stage 3 secretory lysosomes then move into close apposition with the plasma membrane a process known as docking D Stage 4 finally the secretory lysosomes fuse with the plasma membrane releasing their cytotoxic contents towards the target cell plasma membrane 11 CHS Griscelli GS Hermansky- Pudlak HPS CHS CHS 21 CHS Fig 2 23 LYST / CHS1 CHSI 24 GS T ATP ATP 19 Zhang 18 KCN potassium cyanide ATP ATP ATP Fig 2 CHS-16 fibroblasts have enlarged lysosomes P2X7 19 A LAMP-3 staining green in control fibroblasts shows ATP lysosomes distributed throughout the cell arrows B Enlarged view of LAMP-3 in control fibroblasts C LAMP-3 green in CHS-16 fibroblasts shows accumulation volume-activated anion channel VAACs of enlarged lysosomes in the perinuclear area arrows ATP D nlarged view of LAMP-3 in CHS-16 fibroblasts 20 showing some normal-sized lysosomes in the peripheral area arrowheads Co-staining with BODIPY 558 /568 4 phalloidin red visualizes the cell boundaries the DAPI stain blue visualizes the nucleus Scale bars 20 mm Chediak-Higashi 25
4 2 133 Rab27a Ashen Rab27a mrna mirna GS Hermansky-Pudlak - Rab27a Rab27a HPS 15 Rab27a 10 BLOC- Hermansky-Pudak Griscelli Rab27a T ATP AP mrna mirna References 29 1 Holt O J Gallo F Griffiths G M Regulating secretory lysosomes B 30 B Cell Biol Mullins C B biogenesis J Bioessays 32 B 4 Burgoyne R D Morgan A 33 L 34 Mambula - 70 heat shock protein 70 HSP 70 hmunc13-4 J HSP70 LAMP1 35 v- WASp- SRc B 36 mrna mirna 37 Exp Med Bryceson Y T Rudd E Zheng C et al epidermal growth factor receptor EGFR EGFR 38 J J Biochem Blott E J Griffiths G M Secretory lysosomes J Nat Rev Mol Bonifacino J S The molecular machinery for lysosome Physiol Rev Secretory granule exocytosis J 5 Agulhon C Petravicz J McMullen A B et al What is the role of astrocyte alcium in neurophysiology J Neuron Menager M M Ménasché G Romao M et al Secretory cytotoxic granule maturation and exocytosis require the effector protein Nat Immunol Krzewski K Chen X Orange J S et al Formation of a WIP- actin- and myosin IIA-containing multiprotein complex in activated NK cells and its alteration by KIR inhibitory signaling J J Cell Biol Andzelm M M Chen X Krzewski K et al Myosin IIA is required for cytolytic granule exocytosis in human NK cells J J Defective cytotoxic lymphocyte degranulation in syntaxin-11 deficient familial hemophagocytic lymphohistiocytosis 4 FHL4 patients J Blood
5 Marcet-Palacios M Odemuyiwa S O Coughlin J J et al Vesicleassociated membrane protein 7 VAMP-7 is essential for target cell killing in a natural killer cell line J Biochem Biophys Res Commun Topham N J Hewitt E W Natural killer cell cytotoxicity how do they pull the trigger J Immunology Tsuboi T Fukuda M Rab3A and Rab27A cooperatively regulate the docking step of dense-core vesicle exocytosis in PC12 cells J J Cell Sci pt Fukuda M Versatile role of Rab27 in membrane trafficking focus on the Rab27 effector families J J Biochem Tokyo Brzezinska A A Johnson J L Munafo D B The Rab27a effectors JFC1 / Slp1 and Munc13-4 regulate exocytosis of neutrophil granules J Traffic Munafó D B Johnson J L Ellis B A et al Rab27a is a key component of the secretory machinery of a zurophilic granules in granulocytes J Biochem J Tolmachova T Anders R Stinchcombe J et al A general role for Rab27a in secretory cells J Mol Biol Cell Hume AN Collinson LM Rapak A et al Rab27a regulates the peripheral distribution of melanosomes in melanocytes J J Cell Biol Zhang Z Chen G Zhou W et al Regulated ATP release from astrocytes through lysosome exocytosis J Nat Cell Biol Hayashi T Shoji M Abe K Molecular mechanisms of ischemic neuronal cell death -with relevance to Alzheimer s disease J Curr Alzheimer Res Duan S Neary J T P2X 7 receptors properties and relevance to CNS function J Glia Fields R D Ni Y Nonsynaptic communication through ATP release from volume-activated anion channels in axons J Sci Signal ra73 22 Westbroek W Adams D HuizingM et al Cellulardefects in Chediak-Higashi syndrome correlate with the molecular genotype and clinical phenotype J J Invest Dermatol Manoli I Golas G Westbroek W et al Chediak-Higashi syndrome with early developmental delay resulting from paternal heterodisomy of chromosome 1 J Am J Med Genet A A Kaplan J De Domenico I Ward D M Chediak-Higashi syndrome J Curr Opin Hematol Clark R Griffiths G M Lytic granules secretory lysosomes and disease J Curr Opin Immunol Gautam R Novak E K Tan J et al Interaction of Hermansky- Pudlak syndrome genes in the regulation of lysosome-related organelles J Traffic Clark R H Stinchcombe J C Day A et al Adaptor protein 3- dependent microtubule-mediated movement of lytic granules to the immunological synapse J Nat Immunol Fontana S Parolini S Vermi W et al Innate immunity defects in Hermansky-Pudlak type 2 syndrome J Blood Mohamed M M Sloane B F Cysteine cathepsins multifunctional enzymes in cancer J Nat Reviews Cancer Krueger S Haeckel C Buehling F et al A Inhibitory effects of antisense cathepsin B cdna transfection on invasion and motility in a human osteosarcoma cell line J Cancer Res Roshy S Sloane B F Moin K Pericellular cathepsin B and malignant progression J Cancer Metastasis Rev Yanamandra N Gumidyala KV Waldron KG et al Blockade of cathepsin B expression in human glioblastoma cells is associated with suppression of angiogenesis J Oncogene Szpaderska A M Silberman S Ahmed Y et al A Sp1 regulates cathepsin B transcription and invasiveness in murine B16 melanoma cells J Anticancer Res Roth W Deussing J Botchkarev VA et al Cathepsin L deficiency as molecular defect of furless hyperproliferation of keratinocytes and pertubation of hair follicle cycling J FASEB J Mambula S S Calderwood SK Heat shock protein 70 is secreted from tumor cells by a nonclassical pathway involving lysosomal endosomes J J Immunol Tu C Ortega-Cava C F Chen G et al Lysosomal cathepsin B participates in the podosome -mediated extracellular matrix degradation and invasion via secreted lysosomes in v-sc fibroblasts J Cancer Res Skog J Würdinger T van Rijn S et al Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers J Nat Cell Biol Al-Nedawi K Meehan B Micallef J et al Intercellular transfer of the oncogenic receptor EGFRvⅢ by microvesicles derived from tumour cells J Nat Cell Biol McCarthy N Biol Oncogenesis A sideways move J Nat Cell
regulation of polarized membrane transport by rab GTPases Peter van der Sluijs;
regulation of polarized membrane transport by rab GTPases Peter van der Sluijs; p.vandersluijs@umcutrecht.nl cytotoxic T cell recognizes complex of viral peptide with MHC class I and kills infected cell
More informationRegulating Secretory Lysosomes
JB Minireview Membrane Traffic in Physiology and Pathology J. Biochem. 140, 7 12 (2006) doi:10.1093/jb/mvj126 Regulating Secretory Lysosomes Oliver J. Holt, Federico Gallo and Gillian M. Griffiths* Sir
More informationProject report October 2012 March 2013 CRF fellow: Principal Investigator: Project title:
Project report October 2012 March 2013 CRF fellow: Gennaro Napolitano Principal Investigator: Sergio Daniel Catz Project title: Small molecule regulators of vesicular trafficking to enhance lysosomal exocytosis
More informationSubcellular biochemistry
Department of Medical Biochemistry Semmelweis University Subcellular biochemistry February-March 2017 Subcellular biochemistry (biochemical aspects of cell biology) Miklós Csala Semmelweis University Dept.
More informationImmunodeficiencies and Genetic Mutations Affecting NK Cells in Humans Prof. Jordan Orange MD/PhD
Immunodeficiencies and Genetic Mutations Assistant Professor of Pediatrics University of Pennsylvania School of Medicine Children s Hospital of Philadelphia 1 NK cells NK cells are lymphocytes important
More informationCELLS. Cells. Basic unit of life (except virus)
Basic unit of life (except virus) CELLS Prokaryotic, w/o nucleus, bacteria Eukaryotic, w/ nucleus Various cell types specialized for particular function. Differentiation. Over 200 human cell types 56%
More informationMolecular Cell Biology - Problem Drill 17: Intracellular Vesicular Traffic
Molecular Cell Biology - Problem Drill 17: Intracellular Vesicular Traffic Question No. 1 of 10 1. Which of the following statements about clathrin-coated vesicles is correct? Question #1 (A) There are
More informationBME NEUROSCIENCE PRINCIPLES OF NEURAL SCIENCE 1 ST SEMESTER GRADUATE COURSE HYOUNG F. KIM
BME NEUROSCIENCE PRINCIPLES OF NEURAL SCIENCE 1 ST SEMESTER GRADUATE COURSE HYOUNG F. KIM BASIC CONCEPT OF CELL BIOLOGY CELL & NEURON What are the differences? BASIC CONCEPT OF CELL 1. What are the cells
More informationT cell-mediated immunity
T cell-mediated immunity Overview For microbes within phagosomes in phagocytes.cd4+ T lymphocytes (TH1) Activate phagocyte by cytokines studies on Listeria monocytogenes For microbes infecting and replicating
More informationResearch Article A Modified NK Cell Degranulation Assay Applicable for Routine Evaluation of NK Cell Function
Immunology Research Volume 2016, Article ID 3769590, 6 pages http://dx.doi.org/10.1155/2016/3769590 Research Article A odified NK Cell Degranulation Assay Applicable for Routine Evaluation of NK Cell Function
More informationWhat Cell Make Up the Brain and Spinal Cord
What Cell Make Up the Brain and Spinal Cord Jennifer LaVail, Ph.D. (http://anatomy.ucsf.edu/pages/lavaillab/index.html) What kinds of cells are these?" Neuron?" Epithelial cell?" Glial cell?" What makes
More informationendomembrane system internal membranes origins transport of proteins chapter 15 endomembrane system
endo system chapter 15 internal s endo system functions as a coordinated unit divide cytoplasm into distinct compartments controls exocytosis and endocytosis movement of molecules which cannot pass through
More informationCell Quality Control. Peter Takizawa Department of Cell Biology
Cell Quality Control Peter Takizawa Department of Cell Biology Cellular quality control reduces production of defective proteins. Cells have many quality control systems to ensure that cell does not build
More informationDISSERTATION DETERMINATION OF THE FUNCTIONS OF RAB32, RAB38, AND THEIR EFFECTOR MYOSIN VC IN THE BIOGENESIS OF MELANOSOMES.
DISSERTATION DETERMINATION OF THE FUNCTIONS OF RAB32, RAB38, AND THEIR EFFECTOR MYOSIN VC IN THE BIOGENESIS OF MELANOSOMES Submitted by Jarred Bultema Department of Biochemistry and Molecular Biology In
More informationLong-term consumption of Carnosine contributes to improving effector functions of NK cells
International Journal of Science Vol.5 No.7 218 ISSN: 1813-489 Long-term consumption of arnosine contributes to improving effector functions of NK cells bstract Guangao Liu ollege of Life Science and Technology,
More informationAntigen Presentation and T Lymphocyte Activation. Abul K. Abbas UCSF. FOCiS
1 Antigen Presentation and T Lymphocyte Activation Abul K. Abbas UCSF FOCiS 2 Lecture outline Dendritic cells and antigen presentation The role of the MHC T cell activation Costimulation, the B7:CD28 family
More informationEpstein Barr. hemophagocytic lymphohistiocytosis HLH FHL X EBV AHS EBV HLH NK HLH EBV HLH HLH FHL 2 HLH HLH EBV HLH
2010 Vol. 22No. 159 41 1 EpsteinBarr hemophagocytic lymphohistiocytosishlh EB HLH 3 1 HLHFHL HLH EBV 2 X XLPSAP 3 EBVHLH EB CAEBV 2 T NK EBV HLH 1 HLH HLHFHL 2 HLH EB EB HLH EBVHLH FHLX EBVAHS EBVHLHNK
More informationSupplemental Information. Ca V 2.2 Gates Calcium-Independent. but Voltage-Dependent Secretion. in Mammalian Sensory Neurons
Neuron, Volume 96 Supplemental Information Ca V 2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons Zuying Chai, Changhe Wang, Rong Huang, Yuan Wang, Xiaoyu Zhang,
More informationMolecular Trafficking
SCBM 251 Molecular Trafficking Assoc. Prof. Rutaiwan Tohtong Department of Biochemistry Faculty of Science rutaiwan.toh@mahidol.ac.th Lecture outline 1. What is molecular trafficking? Why is it important?
More informationPediatric Hemophagocytic Syndromes: A Diagnostic and Therapeutic Challenge
Review Pediatric Hemophagocytic Syndromes: A Diagnostic and Therapeutic Challenge Nada Jabado, MD, PhD; Christine McCusker, MD; Genevieve de Saint Basile, MD, PhD Abstract Pediatric hemophagocytic syndrome
More informationChapter 13: Vesicular Traffic
Chapter 13: Vesicular Traffic Know the terminology: ER, Golgi, vesicle, clathrin, COP-I, COP-II, BiP, glycosylation, KDEL, microtubule, SNAREs, dynamin, mannose-6-phosphate, M6P receptor, endocytosis,
More informationT cells III: Cytotoxic T lymphocytes and natural killer cells
T cells III: Cytotoxic T lymphocytes and natural killer cells Margrit Wiesendanger Division of Rheumatology, CUMC September 17, 2008 Killer cells: CD8 + T cells (adaptive) vs. natural killer (innate) Shared
More informationFrom the DEPARTMENT OF WOMAN AND CHILD HEALTH Karolinska Institutet, Stockholm, Sweden
From the DEPARTMENT OF WOMAN AND CHILD HEALTH Karolinska Institutet, Stockholm, Sweden GENETIC AND CELLULAR STUDIES OF FAMILIAL HEMOPHAGOCYTIC LYMPHOHISTIOCYTOSIS Eva Rudd Stockholm 2007 Published and
More informationStructures in Cells. Cytoplasm. Lecture 5, EH1008: Biology for Public Health, Biomolecules
Structures in Cells Lecture 5, EH1008: Biology for Public Health, Biomolecules Limian.zheng@ucc.ie 1 Cytoplasm Nucleus Centrioles Cytoskeleton Cilia Microvilli 2 Cytoplasm Cellular material outside nucleus
More informationMicro 204. Cytotoxic T Lymphocytes (CTL) Lewis Lanier
Micro 204 Cytotoxic T Lymphocytes (CTL) Lewis Lanier Lewis.Lanier@ucsf.edu Lymphocyte-mediated Cytotoxicity CD8 + αβ-tcr + T cells CD4 + αβ-tcr + T cells γδ-tcr + T cells Natural Killer cells CD8 + αβ-tcr
More informationCell Overview. Hanan Jafar BDS.MSc.PhD
Cell Overview Hanan Jafar BDS.MSc.PhD THE CELL is made of: 1- Nucleus 2- Cell Membrane 3- Cytoplasm THE CELL Formed of: 1. Nuclear envelope 2. Chromatin 3. Nucleolus 4. Nucleoplasm (nuclear matrix) NUCLEUS
More informationDefects of Innate Immunity
Defects of Innate Immunity TLR signalling IRAK4 and MyD88 AR Same clinical phenotype for both genes (Recurrent) invasive bacterial infections: Meningitis and septicemia >> skin Pneumococcus >> Staph aureus
More information(a) Significant biological processes (upper panel) and disease biomarkers (lower panel)
Supplementary Figure 1. Functional enrichment analyses of secretomic proteins. (a) Significant biological processes (upper panel) and disease biomarkers (lower panel) 2 involved by hrab37-mediated secretory
More informationSYNAPTIC COMMUNICATION
BASICS OF NEUROBIOLOGY SYNAPTIC COMMUNICATION ZSOLT LIPOSITS 1 NERVE ENDINGS II. Interneuronal communication 2 INTERNEURONAL COMMUNICATION I. ELECTRONIC SYNAPSE GAP JUNCTION II. CHEMICAL SYNAPSE SYNAPSES
More informationMedical Virology Immunology. Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University
Medical Virology Immunology Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University Human blood cells Phases of immune responses Microbe Naïve
More informationWiskott-Aldrich Syndrome: a cytoskeleton disease
Wiskott-Aldrich Syndrome: a cytoskeleton disease Elie Haddad, MD, PhD Pediatric Immunology and Rheumatology CHU Ste-Justine Université de Montréal, Montreal, QC, Canada Wiskott-Aldrich Syndrome A disease
More informationHemophagocytic Lymphohistiocytosis Secondary to T cell/histiocyte-rich Large B-cell Lymphoma
Hemophagocytic Lymphohistiocytosis Secondary to T cell/histiocyte-rich Large B-cell Lymphoma Katherine Devitt, M.D., Benjamin Chen, M.D., Ph.D., Hongbo Yu, M.D., Ph.D., Bruce Woda, M.D. 1 1 Department
More informationACTIVATION OF T LYMPHOCYTES AND CELL MEDIATED IMMUNITY
ACTIVATION OF T LYMPHOCYTES AND CELL MEDIATED IMMUNITY The recognition of specific antigen by naïve T cell induces its own activation and effector phases. T helper cells recognize peptide antigens through
More informationStructures in Cells. Lecture 5, EH1008: Biology for Public Health, Biomolecules.
Structures in Cells Lecture 5, EH1008: Biology for Public Health, Biomolecules Limian.zheng@ucc.ie 1 Cytoplasm Nucleus Centrioles Cytoskeleton Cilia Microvilli 2 Cytoplasm Cellular material outside nucleus
More informationTHE SYNAPTIC VESICLE CYCLE
Annu. Rev. Neurosci. 2004. 27:509 47 doi: 10.1146/annurev.neuro.26.041002.131412 Copyright c 2004 by Annual Reviews. All rights reserved First published online as a Review in Advance on March 12, 2004
More informationCYTOKINE RECEPTORS AND SIGNAL TRANSDUCTION
CYTOKINE RECEPTORS AND SIGNAL TRANSDUCTION What is Cytokine? Secreted popypeptide (protein) involved in cell-to-cell signaling. Acts in paracrine or autocrine fashion through specific cellular receptors.
More informationInnate Immunity. Natural or native immunity
Innate Immunity 1 Innate Immunity Natural or native immunity 2 When microbes enter in the body 3 Secondly, it also stimulates the adaptive immune system 4 Immunologic memory 5 Components of Innate Immunity
More informationImmunology Basics Relevant to Cancer Immunotherapy: T Cell Activation, Costimulation, and Effector T Cells
Immunology Basics Relevant to Cancer Immunotherapy: T Cell Activation, Costimulation, and Effector T Cells Andrew H. Lichtman, M.D. Ph.D. Department of Pathology Brigham and Women s Hospital and Harvard
More informationT cell and Cell-mediated immunity
T cell and Cell-mediated immunity ( 第十章 第十二章第十二章 ) Lu Linrong ( 鲁林荣 ) PhD Laboratory of Immune Regulation Institute of Immunology Zhejiang University, School of Medicine Medical Research Building B815-819
More informationWe are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists. International authors and editors
We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists 3,900 116,000 120M Open access books available International authors and editors Downloads Our
More informationCell Mediated Immunity CELL MEDIATED IMMUNITY. Basic Elements of Cell Mediated Immunity (CMI) Antibody-dependent cell-mediated cytotoxicity (ADCC)
Chapter 16 CELL MEDIATED IMMUNITY Cell Mediated Immunity Also known as Cellular Immunity or CMI The effector phase T cells Specificity for immune recognition reactions TH provide cytokines CTLs do the
More informationModule 3 Lecture 7 Endocytosis and Exocytosis
Module 3 Lecture 7 Endocytosis and Exocytosis Endocytosis: Endocytosis is the process by which cells absorb larger molecules and particles from the surrounding by engulfing them. It is used by most of
More informationulation of NK cells that retain the capability of expressing the HNK-1 differentiation antigen. Children with the Chediak-Higashi (CH)' syndrome,
RAPID PUBLICATIONS Natural Killer (HNK-1l) Cells in Chediak-Higashi Patients Are Present in Numbers but Are Abnormal in Function and Morphology TORu ABO, JOHN C. RODER, WATARU ABO, MAX D. COOPER, and CHARLES
More informationThe Cytoplasm Li Shulei Department of Histology & Embryology
The Cytoplasm Li Shulei lishulei@tom.com Department of Histology & Embryology Cell components Cytoplasm Plasma membrane Organelles Cytoplasmic deposits Cytoskeleton Cytosol ( Matrix ) Nucleus Plasma membrane
More informationSupplementary Information. Deciphering the Venomic Transcriptome of Killer- Wasp Vespa velutina
Supplementary Information Deciphering the Venomic Transcriptome of Killer- Wasp Vespa velutina Zhirui Liu, Shuanggang Chen, You Zhou, Cuihong Xie, Bifeng Zhu, Huming Zhu, Shupeng Liu, Wei Wang, Hongzhuan
More informationAppendix: Subcellular localization of MIG-14::GFP in C. elegans body wall muscle cells. Pei-Tzu Yang and Hendrik C. Korswagen
Appendix: Subcellular localization of MIG-14::GFP in C. elegans body wall muscle cells Pei-Tzu Yang and Hendrik C. Korswagen 129 SUBCELLULAR LOCALIZATION OF MIG-14 Abstract MIG-14/Wls is a Wnt binding
More informationAcid sphingomyelinase is a critical regulator of cytotoxic granule secretion by
Supplementary Data Acid sphingomyelinase is a critical regulator of cytotoxic granule secretion by primary T lymphocytes Jasmin Herz, Julian Pardo, Hamid Kashkar, Michael Schramm, Elza Kuzmenkina, Erik
More informationChapter 3 Part 2! Pages (10 th and 11 th eds.)! The Cellular Level of Organization! Cellular Organelles and Protein Synthesis!
Chapter 3 Part 2! Pages 65 89 (10 th and 11 th eds.)! The Cellular Level of Organization! Cellular Organelles and Protein Synthesis! The Cell Theory! Living organisms are composed of one or more cells.!
More informationIL-17 in health and disease. March 2014 PSO13-C051n
IL-17 in health and disease March 2014 PSO13-C051n Originally Researchers Suggested That IL-12 and IL-4 drove Th Cell Differentiation Naïve CD4 + T cell Question: Which of these cell types is responsible
More informationVesicle Transport. Vesicle pathway: many compartments, interconnected by trafficking routes 3/17/14
Vesicle Transport Vesicle Formation Curvature (Self Assembly of Coat complex) Sorting (Sorting Complex formation) Regulation (Sar1/Arf1 GTPases) Fission () Membrane Fusion SNARE combinations Tethers Regulation
More informationAdvanced Cell Biology. Lecture 33
Advanced Cell Biology. Lecture 33 Alexey Shipunov Minot State University April 22, 2013 Shipunov (MSU) Advanced Cell Biology. Lecture 33 April 22, 2013 1 / 38 Outline Questions and answers Intracellular
More informationSupplementary Fig. 1 V-ATPase depletion induces unique and robust phenotype in Drosophila fat body cells.
Supplementary Fig. 1 V-ATPase depletion induces unique and robust phenotype in Drosophila fat body cells. a. Schematic of the V-ATPase proton pump macro-complex structure. The V1 complex is composed of
More informationTumor Associated Macrophages as a Novel Target for Cancer Therapy
Tumor mass Tumor Associated Macrophage Tumor Associated Macrophages as a Novel Target for Cancer Therapy This booklet contains forward-looking statements that are based on Amgen s current expectations
More informationAntigen Presentation to T lymphocytes
Antigen Presentation to T lymphocytes Immunology 441 Lectures 6 & 7 Chapter 6 October 10 & 12, 2016 Jessica Hamerman jhamerman@benaroyaresearch.org Office hours by arrangement Antibodies and T cell receptors
More informationOctober 26, Lecture Readings. Vesicular Trafficking, Secretory Pathway, HIV Assembly and Exit from Cell
October 26, 2006 Vesicular Trafficking, Secretory Pathway, HIV Assembly and Exit from Cell 1. Secretory pathway a. Formation of coated vesicles b. SNAREs and vesicle targeting 2. Membrane fusion a. SNAREs
More informationBIO 5099: Molecular Biology for Computer Scientists (et al)
BIO 5099: Molecular Biology for Computer Scientists (et al) Lecture 15: Being a Eukaryote: From DNA to Protein, A Tour of the Eukaryotic Cell. Christiaan van Woudenberg Being A Eukaryote Basic eukaryotes
More informationMechanism of Vesicular Transport
Mechanism of Vesicular Transport Transport vesicles play a central role in the traffic of molecules between different membrane-enclosed enclosed compartments. The selectivity of such transport is therefore
More informationGeneral information. Cell mediated immunity. 455 LSA, Tuesday 11 to noon. Anytime after class.
General information Cell mediated immunity 455 LSA, Tuesday 11 to noon Anytime after class T-cell precursors Thymus Naive T-cells (CD8 or CD4) email: lcoscoy@berkeley.edu edu Use MCB150 as subject line
More informationScott Abrams, Ph.D. Professor of Oncology, x4375 Kuby Immunology SEVENTH EDITION
Scott Abrams, Ph.D. Professor of Oncology, x4375 scott.abrams@roswellpark.org Kuby Immunology SEVENTH EDITION CHAPTER 13 Effector Responses: Cell- and Antibody-Mediated Immunity Copyright 2013 by W. H.
More informationBIOL 4374/BCHS 4313 Cell Biology Exam #2 March 22, 2001
BIOL 4374/BCHS 4313 Cell Biology Exam #2 March 22, 2001 SS# Name This exam is worth a total of 100 points. The number of points each question is worth is shown in parentheses. Good luck! 1. (2) In the
More informationCells: The Living Units
Chapter 3 Part B Cells: The Living Units Annie Leibovitz/Contact Press Images PowerPoint Lecture Slides prepared by Karen Dunbar Kareiva Ivy Tech Community College 3.4 Active Membrane Transport Two major
More informationT cell and Cell-mediated immunity
T cell and Cell-mediated immunity Lu Linrong ( 鲁林荣 ) PhD Laboratory of Immune Regulation Institute of Immunology Zhejiang University, it School of Medicine i Medical Research Building B815-819 Email: Lu.Linrong@gmail.com
More informationMolecular Cell Biology 5068 In class Exam 1 October 2, Please print your name: Instructions:
Molecular Cell Biology 5068 In class Exam 1 October 2, 2012 Exam Number: Please print your name: Instructions: Please write only on these pages, in the spaces allotted and not on the back. Write your number
More informationBIO 5099: Molecular Biology for Computer Scientists (et al) Lecture 15: Being A Eukaryote. Eukaryotic Cells. Basic eukaryotes have:
BIO 5099: Molecular Biology for Computer Scientists (et al) Lecture 15: Being a Eukaryote: From DNA to Protein, A Tour of the Eukaryotic Cell. Christiaan van Woudenberg Being A Eukaryote Basic eukaryotes
More informationCell Structure. Present in animal cell. Present in plant cell. Organelle. Function. strength, resist pressure created when water enters
Cell Structure Though eukaryotic cells contain many organelles, it is important to know which are in plant cells, which are in animal cells and what their functions are. Organelle Present in plant cell
More informationThe Cell Organelles. Eukaryotic cell. The plasma membrane separates the cell from the environment. Plasma membrane: a cell s boundary
Eukaryotic cell The Cell Organelles Enclosed by plasma membrane Subdivided into membrane bound compartments - organelles One of the organelles is membrane bound nucleus Cytoplasm contains supporting matrix
More informationCells and Tissues 3PART A. PowerPoint Lecture Slide Presentation by Patty Bostwick-Taylor, Florence-Darlington Technical College
PowerPoint Lecture Slide Presentation by Patty Bostwick-Taylor, Florence-Darlington Technical College Cells and Tissues 3PART A Cells and Tissues Carry out all chemical activities needed to sustain life
More informationPost-transcriptional regulation of an intronic microrna
Post-transcriptional regulation of an intronic microrna Carl Novina Dana-Farber Cancer Institute Harvard Medical School Broad Institute of Harvard and MIT Qiagen Webinar 05-17-11 Outline 1. The biology
More informationFrom the Department of Medicine Karolinska Institutet, Stockholm, Sweden
From the Department of Medicine Karolinska Institutet, Stockholm, Sweden Role of Syntaxin-11 and Munc18-2 in lymphocyte cytotoxic granule exocytosis Martha-Lena Müller Stockholm 2016 Front cover: Watercolor
More informationStructure and Function of Antigen Recognition Molecules
MICR2209 Structure and Function of Antigen Recognition Molecules Dr Allison Imrie allison.imrie@uwa.edu.au 1 Synopsis: In this lecture we will examine the major receptors used by cells of the innate and
More informationChapter 2 Cell. Zhou Li Prof. Dept. of Histology and Embryology
Chapter 2 Cell Zhou Li Prof. Dept. of Histology and Embryology The inner life of the cell Ⅰ. Plasma membrane (Plasmalemma) 1.1 The structure Unit membrane: inner layer 3-layered structure outer layer mediat
More informationSUPPLEMENTARY FIGURE LEGENDS
SUPPLEMENTARY FIGURE LEGENDS Supplemental FIG. 1. Localization of myosin Vb in cultured neurons varies with maturation stage. A and B, localization of myosin Vb in cultured hippocampal neurons. A, in DIV
More informationM.Sc. III Semester Biotechnology End Semester Examination, 2013 Model Answer LBTM: 302 Advanced Immunology
Code : AS-2246 M.Sc. III Semester Biotechnology End Semester Examination, 2013 Model Answer LBTM: 302 Advanced Immunology A. Select one correct option for each of the following questions:- 2X10=10 1. (b)
More informationCells. 1. Smallest living structures. 2. Basic structural and functional units of the body. 3. Derived from pre-existing cells. 4. Homeostasis.
Cells The Cell The human body has about 75 trillion cells All tissues and organs are made up of cells Smallest functional unit of life Cytology Histology Cytology Epithelial cells Fibroblasts Erythrocytes
More informationThe development of T cells in the thymus
T cells rearrange their receptors in the thymus whereas B cells do so in the bone marrow. The development of T cells in the thymus The lobular/cellular organization of the thymus Immature cells are called
More information5/1/13. The proportion of thymus that produces T cells decreases with age. The cellular organization of the thymus
T cell precursors migrate from the bone marrow via the blood to the thymus to mature 1 2 The cellular organization of the thymus The proportion of thymus that produces T cells decreases with age 3 4 1
More informationInnate Immunity. Natural or native immunity
Innate Immunity 1 Innate Immunity Natural or native immunity 2 When microbes enter in the body 3 Secondly, it also stimulates the adaptive immune system 4 Immunologic memory 5 Components of Innate Immunity
More informationcell movement and neuronal migration
cell movement and neuronal migration Paul Letourneau letou001@umn.edu Chapter 16; The Cytoskeleton; Molecular Biology of the Cell, Alberts et al. 1 Cell migration Cell migration in 3 steps; protrusion,
More informationOrganelles of the Cell & How They Work Together. Packet #7
Organelles of the Cell & How They Work Together Packet #7 Introduction Introduction Organization of cells is basically similar in all cells. Additionally, most cells are tiny Ranging from 1 1000 cubic
More informationCELL PARTS TYPICAL ANIMAL CELL
AP BIOLOGY CText Reference, Campbell v.8, Chapter 6 ACTIVITY1.12 NAME DATE HOUR CELL PARTS TYPICAL ANIMAL CELL ENDOMEMBRANE SYSTEM TYPICAL PLANT CELL QUESTIONS: 1. Write the name of the cell part in the
More informationNeuronal plasma membrane
ORGANELLES ORGANELLES Neuronal plasma membrane The neuronal plasma membrane contains several local domains with unique properties Presynaptic terminal Endoplasmic Reticulum In neurons the Nissl bodies
More informationMolecular Cell Biology 5068 In Class Exam 1 October 3, 2013
Molecular Cell Biology 5068 In Class Exam 1 October 3, 2013 Exam Number: Please print your name: Instructions: Please write only on these pages, in the spaces allotted and not on the back. Write your number
More informationPractice Exam 2 MCBII
1. Which feature is true for signal sequences and for stop transfer transmembrane domains (4 pts)? A. They are both 20 hydrophobic amino acids long. B. They are both found at the N-terminus of the protein.
More informationHLA and antigen presentation. Department of Immunology Charles University, 2nd Medical School University Hospital Motol
HLA and antigen presentation Department of Immunology Charles University, 2nd Medical School University Hospital Motol MHC in adaptive immunity Characteristics Specificity Innate For structures shared
More informationT Cell Effector Mechanisms I: B cell Help & DTH
T Cell Effector Mechanisms I: B cell Help & DTH Ned Braunstein, MD The Major T Cell Subsets p56 lck + T cells γ δ ε ζ ζ p56 lck CD8+ T cells γ δ ε ζ ζ Cα Cβ Vα Vβ CD3 CD8 Cα Cβ Vα Vβ CD3 MHC II peptide
More informationChapter 3: Cells. I. Overview
Chapter 3: Cells I. Overview A. Characteristics 1. Basic structural/functional unit 2. Diameter is too small to see by the naked eye 3. Can be over 3 feet long 4. Trillions of cells in over 200 basic types
More informationAP Biology Cells: Chapters 4 & 5
AP Biology Cells: Chapters 4 & 5 Multiple Choice Identify the choice that best completes the statement or answers the question. 1. The was the first unifying principle of biology. a. spontaneous generation
More informationThe Major Histocompatibility Complex (MHC)
The Major Histocompatibility Complex (MHC) An introduction to adaptive immune system before we discuss MHC B cells The main cells of adaptive immune system are: -B cells -T cells B cells: Recognize antigens
More informationCELL BIOLOGY - CLUTCH CH THE IMMUNE SYSTEM.
!! www.clutchprep.com CONCEPT: OVERVIEW OF HOST DEFENSES The human body contains three lines of against infectious agents (pathogens) 1. Mechanical and chemical boundaries (part of the innate immune system)
More informationCell Cell
Go to cellsalive.com. Select Interactive Cell Models: Plant and Animal. Fill in the information on Plant and Animal Organelles, then Click on Start the Animation Select Plant or Animal Cell below the box.
More informationCELLS.
CELLS http://www.aimediaserver.com/studiodaily/harvard/harvard.swf INTERESTING FACTS The longest cells in the human body are the motor neurons. They can be up to 1.37 meters long and go from the spinal
More informationBasis and Clinical Applications of Interferon
Interferon Therapy Basis and Clinical Applications of Interferon JMAJ 47(1): 7 12, 2004 Jiro IMANISHI Professor, Kyoto Prefectural University of Medicine Abstract: Interferon (IFN) is an antiviral substance
More informationIntroduction. Cancer Biology. Tumor-suppressor genes. Proto-oncogenes. DNA stability genes. Mechanisms of carcinogenesis.
Cancer Biology Chapter 18 Eric J. Hall., Amato Giaccia, Radiobiology for the Radiologist Introduction Tissue homeostasis depends on the regulated cell division and self-elimination (programmed cell death)
More informationSupplementary Figure 1. Rab27a-KD inhibits speed and persistence of HEp3 cells migrating in the chick CAM. (a) Western blot analysis of Rab27a
Supplementary Figure 1. Rab27a-KD inhibits speed and persistence of HEp3 cells migrating in the chick CAM. (a) Western blot analysis of Rab27a expression in GFP-expressing HEp3 cells. (b) Representative
More informationMicroRNA expression profiling and functional analysis in prostate cancer. Marco Folini s.c. Ricerca Traslazionale DOSL
MicroRNA expression profiling and functional analysis in prostate cancer Marco Folini s.c. Ricerca Traslazionale DOSL What are micrornas? For almost three decades, the alteration of protein-coding genes
More informationAnatomy Chapter 2 - Cells
Cells Cells are the basic living structural, functional unit of the body Cytology is the branch of science that studies cells The human body has 100 trillion cells 200 different cell types with a variety
More informationMembrane Structure and Function
Chapter 7 Membrane Structure and Function PowerPoint Lecture Presentations for Biology Eighth Edition Neil Campbell and Jane Reece Lectures by Chris Romero, updated by Erin Barley with contributions from
More informationLegionella pneumophila: an intracellular pathogen of phagocytes Prof. Craig Roy
an intracellular pathogen of phagocytes Section of Microbial Pathogenesis, Yale University School of Medicine 1 Legionella pneumophila Gram-negative bacterium Facultative intracellular pathogen Protozoa
More informationOrganelles of the Cell & How They Work Together. Packet #5
Organelles of the Cell & How They Work Together Packet #5 Developed by Mr. Barrow 2018 1 Introduction Organization of cells is basically similar in all cells. Additionally, most cells are tiny Ranging
More informationBiology 12 Cell Structure and Function. Typical Animal Cell
Biology 12 Cell Structure and Function Typical Animal Cell Vacuoles: storage of materials and water Golgi body: a series of stacked disk shaped sacs. Repackaging centre stores, modifies, and packages proteins
More information