SUPPLEMENTARY MATERIAL

Size: px
Start display at page:

Download "SUPPLEMENTARY MATERIAL"

Transcription

1 SUPPLEMENTARY MATERIAL Acetone and methanol fruit extracts of Terminalia paniculata inhibit HIV-1 infection in vitro Ankita Durge a, Pratiksha Jadaun a, Ashish Wadhwani a, Ashish A. Chinchansure b, Madhukar Said b, H.V Thulasiram b, Swati P. Joshi b and Smita S. Kulkarni a * a Department of Virology, National AIDS Research Institute, Pune , India b Division of Organic Chemistry, CSIR-National Chemical Laboratory, Pune, , India *Corresponding author. skulkarni@nariindia.org Abstract In this study, we report the in vitro anti-hiv1 activity of acetone and methanol extracts of fruit of Terminalia paniculata. Cytotoxicity tests were conducted on TZM-bl cells and peripheral blood mononuclear cells (PBMC), the CC 50 values of both the extracts were 260 µg/ml. Using TZM-bl cells, the extracts were tested for their ability to inhibit replication of two primary isolates HIV-1 (X4, Subtype D), HIV-1 (R5, Subtype C). The activity against HIV-1 primary isolate (R5, Subtype C) was confirmed using activated PBMC and by quantification of HIV-1 p24 antigen. Both the extracts showed anti-hiv1 activity in a dose dependent manner. The EC 50 values of the acetone and methanol extracts of T. paniculata were 10.3 µg/ml. The enzymatic assays were performed to determine the mechanism of action which indicated that the anti-hiv1 activity might be due to inhibition of reverse transcriptase ( 77.7% inhibition) and protease ( 69.9% inhibition) enzymes. Keywords: Terminalia paniculata, anti-hiv1 activity, acetone extract, methanol extract, protease, reverse transcriptase

2 Experimental Plant collection and processing Terminalia paniculata fruits were collected from Varandha Ghat, Dist. Pune in December A herbarium sample was deposited in the Botanical Survey of India, Western Circle, Pune (Voucher No. SPTP2N). Fruits were shade dried and pulverized. Preparation of T. paniculata acetone (TPA) extract Pulverized fruits (3000 g) were extracted with acetone (4L 3 14h) at room temperature. The acetone-soluble fractions were filtered and concentrated under reduced pressure to yield a greenish acetone extract (150 g, 5% yield based on dry fruits). Preparation of T. paniculata methanol (TPM) extract TPM extract was prepared from the residue remained after acetone extraction. Residue was extracted with methanol (4L 3 14h) at room temperature. The methanol-soluble fractions were filtered and concentrated under reduced pressure to yield a brownish methanol extract (190 g, 6.33% yield). Cell line TZM-bl cell line was used for primary screening of extracts. The TZM-bl cell line is derived from recombinant HeLa cells containing β-galactosidase and luciferase genes under HIV-1 LTR promoter. The cells were obtained from National Institutes of Health AIDS Research and Reference Reagent Program (NIH ARRRP) and maintained in DMEM (Gibco- Invitrogen, Carlsbad, CA, USA) supplemented with 10% heat inactivated FBS (Moregate, Australia), HEPES (Gibco, USA), penicillin and streptomycin (Gibco, USA). Cultures were incubated at 37 o C in a humidified 5% CO 2 atmosphere and used when 80% cell confluency was achieved. Phytochemical analysis The phytochemical analysis of TPA and TPM extracts was carried out as per the standard methodology (Kokate 1994; Harborne 1998).

3 Virus Stock The primary isolate HIV-1 UG070 (X4, Subtype D from Uganda) was obtained from NIH ARRRP, whereas Indian primary isolates HIV-1 VB59 and HIV-1 VB51 (R5, Subtype C) were isolated and propagated in our laboratory. The viruses were grown in PHA-p (5 µg/ml) activated peripheral blood mononuclear cells (PBMC) derived from healthy donors and quantified by HIV-1 p24 antigen detection assay (Advanced Bioscience Lab [ABL] Inc, USA). These virus stocks were titrated in the TZM-bl cell line and TCID50 (50% Tissue culture infective dose) of each virus stock was determined in the TZM-bl cells and PHA-p PBMC using Spearman Karber formula. Cytotoxicity assays The cytotoxicity of the extracts for TZM-bl cells and activated PBMC was determined using MTT [3-(4,5 dimethyl thiazole-2 yl) - 2,5-diphenyl tetrazolium bromide] (Sigma Aldrich, Inc., USA) assay. Briefly, two-fold dilutions of the working stock (1mg/ml) of each extract were prepared and added on overnight cultured TZM-bl cells ( cells/well). After incubation for 48 h, the cell viability was determined using MTT, the percent viability was determined and the results were expressed as CC 50 (concentration showing 50% cell viability). Assays for anti-hiv1 activity In vitro anti-hiv1 assay was carried out using published protocol (Geonnotti et al. 2014; Singh et al. 2015). Based on the CC 50 value, the anti-hiv1 activity was evaluated using a range of non cytotoxic concentrations of the extracts by following assays. In cell associated assay (CA), the TZM-bl cells ( cells/well) were first infected with the viral stocks (400 TCID 50 ) for 2 h at 37 o C in 5% CO 2 atmosphere before exposing the infected cells to the serial dilutions of extracts 0.78µg/mL to 100µg/mL in duplicate. After 48 h, luciferase activity was measured using Britelite plus reagent (Perkin Elmer, USA) (Singh et al. 2015). While in cell free assay (CF), the viral stocks (400 TCID 50 ) were first treated with the serial dilutions of extracts (0.78µg/mL to 100µg/mL) in duplicate for 1 h, at 37 o C in 5% CO 2 atmosphere preceding to its addition on the TZM-bl cells ( cells/well) and after 48 h, luciferase activity was measured. Azidothymidine (AZT) and Dextran sulfate (DS) were used as positive controls in cell associated and cell free anti-hiv1 assays, respectively. The EC 50

4 (effective concentration showing 50% virus inhibition) was calculated and compared with positive control. Confirmatory Assays using PBMC Briefly, in cell associated assay, PHA-p activated PBMC were infected with HIV-1 VB51 (400 TCID 50 ) and seeded ( cells/well). This was followed by the addition of sub toxic concentrations of extracts (0.78µg/mL to 100µg/mL). After 120 h, the virus supernatant was analyzed for p24 detection as described above. The EC 50 value was calculated and compared with positive control. For cell free assay, the viral stock of HIV-1 VB51 (400 TCID 50 ) was treated with the serial dilutions (0.78µg/mL to 100µg/mL) of extracts and incubated for 1 h. The pretreated virus was added onto activated PBMC ( cells/well) and incubated for 24 h. The next day, cells were washed with PBS and incubated for 120 h at 37 C after adding fresh media. After 120 h, virus supernatant was analyzed for p24 antigen detection as described above. Azidothymidine (AZT) and Dextran sulfate (DS) were used as positive controls in cell associated and cell free anti-hiv1 assays, respectively. HIV reverse transcriptase (RT) and protease assay (PR) The inhibitory action of TPA and TPM extracts on the HIV-1 reverse transcriptase (RT) and protease enzymes was evaluated using commercially available kits (Roche Applied Sciences Mannheim, Germany and Anaspec, CA, USA respectively) by following instructions of the manufacturers.

5 Table S1: In vitro cytotoxicity and anti-hiv1 activity of TPA and TPM extracts using TZM-bl cells and PBMC Details of the extract (*CC 5 0, µg/m L) Anti-HIV activity (**EC 50, µg/ml) TZM-bl PBMC HIV-1 UG070 HIV-1 VB59 HIV-1 VB51 *CC 50 HIV-1 VB51 CA CF CA CF CA CF, CA CF µg/m L) Acetone- Extract Methanol - Extract *CC 50 : The cytotoxic concentration of the extracts that caused the reduction of viable cells by 50%. All data presented are averages of three independent experiments. **EC 50 : The effective concentration of the extracts that resulted in 50% inhibition in HIV-1 infection. All data presented are averages of three independent experiments.

6 Table S2: Phytochemical analysis of TPA and TPM extracts T. paniculata Extracts Code Tannins Glycosid es Flavonoid s Saponins Phytosterol s Triterpen es Alkaloids Acetone Extract Methanol Fraction TPA TPM Key: +: Present in low quantity; ++: Present in moderate quantity; +++: Present in high quantity; - : Absent.

7 Figure S1: Anti-HIV1 activity of TPA and TPM extracts on TZM-bl reporter cell Legend: TZM-bl cell infected with (a, b) HIV-1 UG070 (X4, Subtype D) (c, d) HIV-1 VB59 (R5, Subtype C) and (e, f) HIV-1 VB51 (R5, Subtype C) through cell associated (CA) and cell free (CF) assays. AZT and DS were used as positive controls in CA and CF assays respectively. Data is represented as mean ± SE of three independent experiments

8 Figure S2: Anti-HIV1 activity of TPA and TPM extracts with HIV-1 VB51 infected PBMC (a) cell associated (CA) and (b) cell free (CF) assays Legend: Quantification of HIV-1 p24 antigen following treatment of extracts at various concentrations. AZT and DS were used as positive controls in CA and CF assays respectively. Data is represented as mean ± SE of three independent experiments.

9 Figure S3: Determination of mechanism of action Legend: Enzymatic assays (a) HIV1 RT inhibition assay (b) Protease inhibition assay. HIV-1 RT with AZT treatment and HIV-1 protease with pepstatin treatment were taken as positive controls in the respective experiment. Data is represented as mean ± SE of duplicates experiments. Referances Geonnotti A. R., Bilska M., Yuan X., Ochsenbauer C., Edmonds T. G., Kappes J. C., Liao H. X., Haynes B. F., Montefiori D. C Differential inhibition of human immunodeficiency virus type1 in peripheral blood mononuclear cells and TZM-bl cells by endotoxin mediated chemokine and gamma interferon production. AIDS Research Human Retroviruses, 26: Harborne, A. J Phytochemical methods a guide to modern techniques of plant analysis. Springer Science & Business Media. Kokate C. K Practical pharmacognosy. In Vallabh Prakashan, New Delhi 4: Singh A., Yadav D., Yadav M., Dhamanage A., Kulkarni S., Singh R. K Molecular modeling, synthesis and biological evaluation of N-Heteroaryl Compounds as reverse transcriptase inhibitors against HIV-1. Chemical biology & drug design, 85:

IN VITRO ANTICANCER ACTIVITY OF FLOWER EXTRACTS OF COUROUPITA GUIANENSIS

IN VITRO ANTICANCER ACTIVITY OF FLOWER EXTRACTS OF COUROUPITA GUIANENSIS CHAPTER 3 IN VITRO ANTICANCER ACTIVITY OF FLOWER EXTRACTS OF COUROUPITA GUIANENSIS 3. INTRODUCTION Plants are the basic source of knowledge of modern medicine. Almost all the parts of the plant, namely

More information

8. CHAPTER IV. ANTICANCER ACTIVITY OF BIOSYNTHESIZED SILVER NANOPARTICLES

8. CHAPTER IV. ANTICANCER ACTIVITY OF BIOSYNTHESIZED SILVER NANOPARTICLES 8. CHAPTER IV. ANTICANCER ACTIVITY OF BIOSYNTHESIZED SILVER NANOPARTICLES 8.1. Introduction Nanobiotechnology, an emerging field of nanoscience, utilizes nanobased-systems for various biomedical applications.

More information

In-vitro assay for Cytotoxicity activity in ethonolic extract of fruit rind of Couropita Guianensis aubl

In-vitro assay for Cytotoxicity activity in ethonolic extract of fruit rind of Couropita Guianensis aubl ISSN: 2319-7706 Volume 3 Number 10 (2014) pp. 169-176 http://www.ijcmas.com Original Research Article In-vitro assay for Cytotoxicity activity in ethonolic extract of fruit rind of Couropita Guianensis

More information

International Journal Of Recent Scientific Research

International Journal Of Recent Scientific Research International Journal Of Recent Scientific Research ISSN: 0976-3031 Volume: 7(5) May -2016 NEW ERA ABOUT TREATMENT - EVOLUATION OF ANTICANCER PROPERTIES OF EVERGREEN MEDICINAL PLANT ANNONA MURICATA (GRAVIOLA)

More information

Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals

Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals Isolation of HIV-1 From PBMC of Infected Individuals 17 2 Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals Hanneke Schuitemaker

More information

Data Sheet TIGIT / NFAT Reporter - Jurkat Cell Line Catalog #60538

Data Sheet TIGIT / NFAT Reporter - Jurkat Cell Line Catalog #60538 Data Sheet TIGIT / NFAT Reporter - Jurkat Cell Line Catalog #60538 Background: TIGIT is a co-inhibitory receptor that is highly expressed in Natural Killer (NK) cells, activated CD4+, CD8+ and regulatory

More information

Anti-cancer activity of Aya Thambira Chendooram (ATC) in in-vitro cell line against Breast Carcinoma

Anti-cancer activity of Aya Thambira Chendooram (ATC) in in-vitro cell line against Breast Carcinoma International Journal of Advanced Research in Biological Sciences ISSN: 2348-8069 www.ijarbs.com DOI: 10.22192/ijarbs Coden: IJARQG(USA) Volume 5, Issue 1-2018 Research Article DOI: http://dx.doi.org/10.22192/ijarbs.2018.05.01.010

More information

Journal of Chemical and Pharmaceutical Research, 2017, 9(12): Research Article

Journal of Chemical and Pharmaceutical Research, 2017, 9(12): Research Article Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(12):30-34 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Evaluation of Anticancer Activity of Alphonsea Sclerocarpa

More information

Transfection of Sf9 cells with recombinant Bacmid DNA

Transfection of Sf9 cells with recombinant Bacmid DNA Transposition Bacmid DNA Mini Culturing baculo cells Transfection of Sf9 cells with recombinant Bacmid DNA Amplification of the virus Titration of baculo stocks Testing the expression Transposition 1.

More information

Binding property of HIV p24 and Reverse transcriptase by chalcones from Pongamia pinnata seeds

Binding property of HIV p24 and Reverse transcriptase by chalcones from Pongamia pinnata seeds www.bioinformation.net Volume 14(6) Hypothesis Binding property of HIV p24 and Reverse transcriptase by chalcones from Pongamia pinnata seeds Manikannan Mathaiyan 1, Arumugam Suresh 1*, Rangasamy Balamurugan

More information

ACTG Laboratory Technologist Committee Revised Version 2.0 ACTG Lab Man HIV Quantitative PBMC culture May 2004

ACTG Laboratory Technologist Committee Revised Version 2.0 ACTG Lab Man HIV Quantitative PBMC culture May 2004 HIV QUANTITATIVE PBMC MICROCOCULTURE ASSAY 1 PRINCIPLE The quantitative PBMC micrococulture assay estimates the number of infectious units of HIV per million mononuclear cells (IUPM) in peripheral blood

More information

CHAPTER 6 IN-VITRO PHARMACOLOGICAL STUDIES

CHAPTER 6 IN-VITRO PHARMACOLOGICAL STUDIES 95 CHAPTER 6 IN-VITRO PHARMACOLOGICAL STUDIES The biological experiments like in-vitro and in-vivo screening models for various diseases may be applied to discover new molecules. These contemporary techniques

More information

Adenovirus Manual 1. Table of Contents. Large Scale Prep 2. Quick MOI Test 4. Infection of MNT-1 Cells 8. Adenovirus Stocks 9

Adenovirus Manual 1. Table of Contents. Large Scale Prep 2. Quick MOI Test 4. Infection of MNT-1 Cells 8. Adenovirus Stocks 9 Adenovirus Manual 1 Table of Contents Large Scale Prep 2 Quick MOI Test 4 TCID 50 Titration 5 Infection of MNT-1 Cells 8 Adenovirus Stocks 9 CAUTION: Always use filter tips and bleach everything!!! Adenovirus

More information

Electronic Supporting Information for

Electronic Supporting Information for Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2015 Electronic Supporting Information for Rhodamine based Turn-On Fluorescent Probe for Pb(II)

More information

In Vitro Antioxidant Activity and Phytochemical Screening of Pholidota articulata

In Vitro Antioxidant Activity and Phytochemical Screening of Pholidota articulata Human Journals Research Article November 2016 Vol.:5, Issue:1 All rights are reserved by Darshan Singh et al. In Vitro Antioxidant Activity and Phytochemical Screening of Pholidota articulata Keywords:

More information

Journal of Chemical and Pharmaceutical Research, 2013, 5(5): Research Article. Anticancer properties of Cissus quandrangularis

Journal of Chemical and Pharmaceutical Research, 2013, 5(5): Research Article. Anticancer properties of Cissus quandrangularis Available online wwwjocprcom Journal of Chemical and Pharmaceutical Research, 13, 5(5):135-139 Research Article ISSN : 975-7384 CODEN(USA) : JCPRC5 Anticancer properties of Cissus quandrangularis Aayush

More information

HCC1937 is the HCC1937-pcDNA3 cell line, which was derived from a breast cancer with a mutation

HCC1937 is the HCC1937-pcDNA3 cell line, which was derived from a breast cancer with a mutation SUPPLEMENTARY INFORMATION Materials and Methods Human cell lines and culture conditions HCC1937 is the HCC1937-pcDNA3 cell line, which was derived from a breast cancer with a mutation in exon 20 of BRCA1

More information

ACTG Laboratory Technologist Committee Revised Version 2.0 ACTG Lab Man HIV Qualitative PBMC Micrococulture 1 June 2004

ACTG Laboratory Technologist Committee Revised Version 2.0 ACTG Lab Man HIV Qualitative PBMC Micrococulture 1 June 2004 HIV QUALITATIVE PBMC MICROCOCULTURE ASSAY 1. PRINCIPLE: 1.1 A co-culture of patient peripheral blood mononuclear cells (PBMC) and uninfected PHA-stimulated PBMCs is maintained under ideal conditions to

More information

HIV-1 p24 ELISA Pair Set Cat#: orb54951 (ELISA Manual)

HIV-1 p24 ELISA Pair Set Cat#: orb54951 (ELISA Manual) HIV-1 p24 ELISA Pair Set Cat#: orb54951 (ELISA Manual) BACKGROUND Human Immunodeficiency Virus ( HIV ) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to

More information

In Vitro Antioxidant and Cytotoxic Analysis of Boerhaavia diffusa Linn.

In Vitro Antioxidant and Cytotoxic Analysis of Boerhaavia diffusa Linn. Ethnobotanical Leaflets 13: 263-68. 2009. In Vitro Antioxidant and Cytotoxic Analysis of Boerhaavia diffusa Linn. Teepica Priya Darsini D* 1, J.M. Sasikumar 1 and Kulandhaivel M. 2 1 Department of Industrial

More information

International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: Vol.9, No.4, pp , 2016

International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: Vol.9, No.4, pp , 2016 International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 0974-4304 Vol.9, No.4, pp 261-265, 2016 A Research Article on Phytochemical and Physicochemical Evaluation of Leaves & Stems of Kalanchoe

More information

BIOTARGET-1 SERUM-FREE MEDIUM

BIOTARGET-1 SERUM-FREE MEDIUM TECHNICAL INFORMATION BIOTARGET-1 SERUM-FREE MEDIUM Cat. No. 05-080-1 Introduction The BIOTARGET-1 formulation has been developed specifically for use with mononuclear cells (lymphocytes and monocytes)

More information

Supporting Information

Supporting Information Supporting Information Identification of Novel ROS Inducers: Quinone Derivatives Tethered to Long Hydrocarbon Chains Yeonsun Hong,, Sandip Sengupta,, Wooyoung Hur, *, Taebo Sim,, * KU-KIST Graduate School

More information

Hepatitis B Antiviral Drug Development Multi-Marker Screening Assay

Hepatitis B Antiviral Drug Development Multi-Marker Screening Assay Hepatitis B Antiviral Drug Development Multi-Marker Screening Assay Background ImQuest BioSciences has developed and qualified a single-plate method to expedite the screening of antiviral agents against

More information

Cinnamomum Essential Oil Prevents DNA Damage- Induced by Doxorubicin on CHO-K1 Cells

Cinnamomum Essential Oil Prevents DNA Damage- Induced by Doxorubicin on CHO-K1 Cells Indonesian Journal of Cancer Chemoprevention, 2017, 8(1): 27-31 ISSN: 2088 0197 Cinnamomum Essential Oil Prevents DNA Damage- Induced by Doxorubicin on CHO-K1 Cells Layung Sekar Sih Wikanthi, Nindi Wulandari,

More information

DRAFT. c 2.G Serological diagnosis of influenza by microneutralization assay 2.G

DRAFT. c 2.G Serological diagnosis of influenza by microneutralization assay 2.G 2.G c 2.G Serological diagnosis of influenza by microneutralization assay Serological methods rarely yield an early diagnosis of acute influenza virus infection. However, the demonstration of a significant

More information

EVALUATION OF ANTI-HIV-1 ACTIVITY FROM CESTRUM NOCTURNUM AERIAL PARTS AND PHYTOCHEMICAL ANALYSIS

EVALUATION OF ANTI-HIV-1 ACTIVITY FROM CESTRUM NOCTURNUM AERIAL PARTS AND PHYTOCHEMICAL ANALYSIS EVALUATION OF ANTI-HIV-1 ACTIVITY FROM CESTRUM NOCTURNUM AERIAL PARTS AND PHYTOCHEMICAL ANALYSIS 1 KHALED RASHED, 2,3 XING-JIE ZHANG, 2,3 MENG-TING LUO, 2 YONG-TANG ZHENG 1 National Research Centre, Pharmacognosy

More information

Human Immunodeficiency Virus type 1 (HIV-1) p24 / Capsid Protein p24 ELISA Pair Set

Human Immunodeficiency Virus type 1 (HIV-1) p24 / Capsid Protein p24 ELISA Pair Set Human Immunodeficiency Virus type 1 (HIV-1) p24 / Capsid Protein p24 ELISA Pair Set Catalog Number : SEK11695 To achieve the best assay results, this manual must be read carefully before using this product

More information

Detailed step-by-step operating procedures for NK cell and CTL degranulation assays

Detailed step-by-step operating procedures for NK cell and CTL degranulation assays Supplemental methods Detailed step-by-step operating procedures for NK cell and CTL degranulation assays Materials PBMC isolated from patients, relatives and healthy donors as control K562 cells (ATCC,

More information

Asian Journal of Research in Pharmaceutical Sciences and Biotechnology

Asian Journal of Research in Pharmaceutical Sciences and Biotechnology Research Article ISSN: 2349 7114 Asian Journal of Research in Pharmaceutical Sciences and Biotechnology Journal home page: www.ajrpsb.com IN VITRO ANTI-TUMOUR ACTIVITY OF VITEX LEUCOXYLON LINN USING MTT

More information

MTS assay in A549 cells

MTS assay in A549 cells Project: VIGO MTS assay in A549 cells Detection of cell viability/activity AUTHORED BY: DATE: Cordula Hirsch 20.01.2014 REVIEWED BY: DATE: Harald Krug 10.04.2014 APPROVED BY: DATE: DOCUMENT HISTORY Effective

More information

2,6,9-Triazabicyclo[3.3.1]nonanes as overlooked. amino-modification products by acrolein

2,6,9-Triazabicyclo[3.3.1]nonanes as overlooked. amino-modification products by acrolein Supplementary Information 2,6,9-Triazabicyclo[3.3.1]nonanes as overlooked amino-modification products by acrolein Ayumi Tsutsui and Katsunori Tanaka* Biofunctional Synthetic Chemistry Laboratory, RIKEN

More information

Proteomic profiling of small-molecule inhibitors reveals dispensability of MTH1 for cancer cell survival

Proteomic profiling of small-molecule inhibitors reveals dispensability of MTH1 for cancer cell survival Supplementary Information for Proteomic profiling of small-molecule inhibitors reveals dispensability of MTH1 for cancer cell survival Tatsuro Kawamura 1, Makoto Kawatani 1, Makoto Muroi, Yasumitsu Kondoh,

More information

Synthesis of Substituted 2H-Benzo[e]indazole-9-carboxylate as Potent Antihyperglycemic Agent that May Act through IRS-1, Akt and GSK-3β Pathways

Synthesis of Substituted 2H-Benzo[e]indazole-9-carboxylate as Potent Antihyperglycemic Agent that May Act through IRS-1, Akt and GSK-3β Pathways Electronic Supplementary Material (ESI) for MedChemComm. This journal is The Royal Society of Chemistry 2016 Supplementary Data Synthesis of Substituted 2H-Benzo[e]indazole-9-carboxylate as Potent Antihyperglycemic

More information

Large Scale Infection for Pooled Screens of shrna libraries

Large Scale Infection for Pooled Screens of shrna libraries Last modified 01/11/09 Large Scale Infection for Pooled Screens of shrna libraries Biao Luo, Glenn Cowley, Michael Okamoto, Tanaz Sharifnia This protocol can be further optimized if cells being used are

More information

Application of μmacs Streptavidin MicroBeads for the analysis of HIV-1 directly from patient plasma

Application of μmacs Streptavidin MicroBeads for the analysis of HIV-1 directly from patient plasma Excerpt from MACS&more Vol 8 1/2004 Application of μmacs Streptavidin MicroBeads for the analysis of HIV-1 directly from patient plasma L. Davis Lupo and Salvatore T. Butera HIV and Retrovirology Branch,

More information

Antioxidant Activity and Anticancer Study on Phytochemicals Extract from Tubers of Gloriosa superba against Human Cancer Cell (Hep-G2)

Antioxidant Activity and Anticancer Study on Phytochemicals Extract from Tubers of Gloriosa superba against Human Cancer Cell (Hep-G2) Antioxidant Activity and Anticancer Study on Phytochemicals Extract from Tubers of Gloriosa superba against Human Cancer Cell (Hep-G2) Simon SE 1* and Jayakumar FA 2 1 Department of Biotechnology, Bharathidasan

More information

A novel quinoline-based two-photon fluorescent probe for detecting Cd 2+ in vitro and in vivo

A novel quinoline-based two-photon fluorescent probe for detecting Cd 2+ in vitro and in vivo Supporting Information A novel quinoline-based two-photon fluorescent probe for detecting Cd 2+ in vitro and in vivo Yiming Li, a,b Hanbao Chong, a Xiangming Meng,* a Shuxin Wang, a Manzhou Zhu a and Qingxiang

More information

Supplemental Materials and Methods Plasmids and viruses Quantitative Reverse Transcription PCR Generation of molecular standard for quantitative PCR

Supplemental Materials and Methods Plasmids and viruses Quantitative Reverse Transcription PCR Generation of molecular standard for quantitative PCR Supplemental Materials and Methods Plasmids and viruses To generate pseudotyped viruses, the previously described recombinant plasmids pnl4-3-δnef-gfp or pnl4-3-δ6-drgfp and a vector expressing HIV-1 X4

More information

NBP Protocol. Orders: Support: Web: NBP

NBP Protocol. Orders: Support: Web:   NBP NBP2-29541 NBP2-29541 Protocol Orders: orders@novusbio.com Support: technical@novusbio.com Web: www.novusbio.com Protocols, Publications, Related Products, Reviews and more: www.novusbio.com/nbp2-29541

More information

Supplementary data Supplementary Figure 1 Supplementary Figure 2

Supplementary data Supplementary Figure 1 Supplementary Figure 2 Supplementary data Supplementary Figure 1 SPHK1 sirna increases RANKL-induced osteoclastogenesis in RAW264.7 cell culture. (A) RAW264.7 cells were transfected with oligocassettes containing SPHK1 sirna

More information

SensoLyte 520 HIV-1 Protease Assay Kit *Fluorimetric*

SensoLyte 520 HIV-1 Protease Assay Kit *Fluorimetric* SensoLyte 520 HIV-1 Protease Assay Kit *Fluorimetric* Catalog # 71147 Kit Size 100 assays (96-well) or 500 assays (384-well) Convenient Format: Complete kit including all the assay components. Optimized

More information

International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: Vol.7, No.2, pp ,

International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: Vol.7, No.2, pp , International Journal of PharmTech Research CODEN (USA): IJPRIF, ISSN: 0974-4304 Vol.7, No.2, pp 238-242, 2014-2015 Cytotoxicity of Ekor naga Leaf (Rhaphidophora pinnata (Lf) Schott) Chloroform Extract

More information

SUPPLEMENTARY MATERIAL Antiradical and antioxidant activity of flavones from Scutellariae baicalensis radix

SUPPLEMENTARY MATERIAL Antiradical and antioxidant activity of flavones from Scutellariae baicalensis radix SUPPLEMENTARY MATERIAL Antiradical and antioxidant activity of flavones from Scutellariae baicalensis radix Dorota Woźniak A, Andrzej Dryś B, and Adam Matkowski* A A Department of Pharmaceutical Biology

More information

colorimetric sandwich ELISA kit datasheet

colorimetric sandwich ELISA kit datasheet colorimetric sandwich ELISA kit datasheet For the quantitative detection of human TNF-alpha in serum, plasma and cell culture supernatants. general information Catalogue Number Product Name Species cross-reactivity

More information

Histological and Physico-chemical Evaluation of Zanthoxylum nitidum Stem Bark

Histological and Physico-chemical Evaluation of Zanthoxylum nitidum Stem Bark Ethnobotanical Leaflets 13: 540-47, 2009. Histological and Physico-chemical Evaluation of Zanthoxylum nitidum Stem Bark Sanjib Bhattacharya 1*, M. Kamaruz Zaman 2, Ashoke K. Ghosh 1 1 Bengal School of

More information

RNA extraction, RT-PCR and real-time PCR. Total RNA were extracted using

RNA extraction, RT-PCR and real-time PCR. Total RNA were extracted using Supplementary Information Materials and Methods RNA extraction, RT-PCR and real-time PCR. Total RNA were extracted using Trizol reagent (Invitrogen,Carlsbad, CA) according to the manufacturer's instructions.

More information

Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set

Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA ELISA Pair Set Catalog Number : SEK001 To achieve the best assay results, this manual must be read carefully before using this product and the assay

More information

TEST REPORT. Anti-viral effect of disinfectant against feline calicivirus

TEST REPORT. Anti-viral effect of disinfectant against feline calicivirus TEST REPORT Anti-viral effect of disinfectant against feline calicivirus 25 th October 2006 Dr Tobias J. Tuthill Faculty of Biological Sciences University of Leeds Leeds LS2 9JT www.fbs.leeds.ac.uk Contents

More information

Supporting Information

Supporting Information Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2015 Supporting Information Enzyme-activatable Probe with a Self-immolative Linker for Rapid and Sensitive

More information

Extracts from Acacia catechu suppress HIV-1 replication by inhibiting the activities of the viral protease and Tat. Nutan et al.

Extracts from Acacia catechu suppress HIV-1 replication by inhibiting the activities of the viral protease and Tat. Nutan et al. Extracts from Acacia catechu suppress HIV-1 replication by inhibiting the activities of the viral protease and Tat Nutan et al. Nutan et al. Virology Journal 213, 1:39 Nutan et al. Virology Journal 213,

More information

Real-Time the Monitoring Mitophagy Process by A Photostable. Fluorescent Mitochondrion-Specific Bioprobe with AIE. Characteristic

Real-Time the Monitoring Mitophagy Process by A Photostable. Fluorescent Mitochondrion-Specific Bioprobe with AIE. Characteristic Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2015 Electronic Supplementary Information Real-Time the Monitoring Mitophagy Process by A Photostable

More information

INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES

INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES Development of standardization of trikatu churna: A spectrophotometric approach Manjesh Thakur*, P. Porwal, P. Pal, K. Shukla and S.C. Mahajan Mahakal

More information

Journal of Chemical and Pharmaceutical Research

Journal of Chemical and Pharmaceutical Research Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(2):144-149 In vitro hepatoprotective activity of ethanolic

More information

Anti-Aging Activity Of Cucurbita moschata Ethanolic Extract Towards NIH3T3 Fibroblast Cells Induced By Doxorubicin

Anti-Aging Activity Of Cucurbita moschata Ethanolic Extract Towards NIH3T3 Fibroblast Cells Induced By Doxorubicin Indonesian Journal of Cancer Chemoprevention, 2016, 7(2): 49-53 ISSN: 2088 0197 Anti-Aging Activity Of Cucurbita moschata Ethanolic Extract Towards NIH3T3 Fibroblast Cells Induced By Doxorubicin Laeli

More information

Comparative Physicochemical and Phyto-Chemical Screening of Glucomap: An Herbal Antidiabetic Tablet

Comparative Physicochemical and Phyto-Chemical Screening of Glucomap: An Herbal Antidiabetic Tablet Comparative Physicochemical and Phyto-Chemical Screening of Glucomap: An Herbal Antidiabetic Tablet Satyaendra K. Shrivastava* 1, P.K. Dubey 1, B. Shrivastava 2, Pankaj Sharma 2 * 1 Swami Vivekanand College

More information

WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES. World Journal of Pharmacy and Pharmaceutical Sciences SJIF Impact Factor 6.

WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES. World Journal of Pharmacy and Pharmaceutical Sciences SJIF Impact Factor 6. WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES Neeru et al. SJIF Impact Factor 6.041 Volume 5, Issue 6, 1884-1891 Research Article ISSN 2278 4357 DETERMINATION OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID

More information

The Microbiology and Virology Laboratory. Volume --- FINAL REPORT VIRUCIDAL EFFICACY TEST USING SWINE INFLUENZA A VIRUS (H1N1) Test Agent Anolyte

The Microbiology and Virology Laboratory. Volume --- FINAL REPORT VIRUCIDAL EFFICACY TEST USING SWINE INFLUENZA A VIRUS (H1N1) Test Agent Anolyte MICRO I TEST The Microbiology and Virology Laboratory Volume --- FINAL REPORT VIRUCIDAL EFFICACY TEST USING SWINE INFLUENZA A VIRUS (H1N1) Test Agent Anolyte Lot Numbers Oct 4, 2009 Sept 25, 2009 Data

More information

Development of a near-infrared fluorescent probe for monitoring hydrazine in serum and living cells

Development of a near-infrared fluorescent probe for monitoring hydrazine in serum and living cells Supporting Information for Development of a near-infrared fluorescent probe for monitoring hydrazine in serum and living cells Sasa Zhu, Weiying Lin,* Lin Yuan State Key Laboratory of Chemo/Biosensing

More information

Protocol for Gene Transfection & Western Blotting

Protocol for Gene Transfection & Western Blotting The schedule and the manual of basic techniques for cell culture Advanced Protocol for Gene Transfection & Western Blotting Schedule Day 1 26/07/2008 Transfection Day 3 28/07/2008 Cell lysis Immunoprecipitation

More information

B16-F10 (Mus musculus skin melanoma), NCI-H460 (human non-small cell lung cancer

B16-F10 (Mus musculus skin melanoma), NCI-H460 (human non-small cell lung cancer Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2017 Experimental Methods Cell culture B16-F10 (Mus musculus skin melanoma), NCI-H460 (human non-small

More information

Pre-made Lentiviral Particles for Fluorescent Proteins

Pre-made Lentiviral Particles for Fluorescent Proteins Pre-made Lentiviral Particles for Fluorescent Proteins Catalog# Product Name Amounts Fluorescent proteins expressed under sucmv promoter: LVP001 LVP001-PBS LVP002 LVP002-PBS LVP011 LVP011-PBS LVP012 LVP012-PBS

More information

Anti-Aging Skin Care Regimen. Phyto-Stem Cell: Advanced Plant Cell Culture Technology by Bio-FD&C

Anti-Aging Skin Care Regimen. Phyto-Stem Cell: Advanced Plant Cell Culture Technology by Bio-FD&C Anti-Aging Skin Care Regimen Phyto-Stem Cell: Advanced Plant Cell Culture Technology by Bio-FD&C 01 Plant Cell Culture Technology Highly expression of phytochemicals from plant cell culturing process by

More information

BaculoELISA Titer Kit User Manual

BaculoELISA Titer Kit User Manual User Manual Cat. No. 631412 PT3953-1 (PR752243) Published 5 May 2007 Table of Contents I. Introduction...3 II. III. IV. List of Components...4 Additional Materials Required...5 BaculoELISA Titer Kit Protocol

More information

Islet viability assay and Glucose Stimulated Insulin Secretion assay RT-PCR and Western Blot

Islet viability assay and Glucose Stimulated Insulin Secretion assay RT-PCR and Western Blot Islet viability assay and Glucose Stimulated Insulin Secretion assay Islet cell viability was determined by colorimetric (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay using CellTiter

More information

PROTOCOL: OPTIMIZATION OF LENTIVIRAL TRANSDUCTION USING SPINFECTION

PROTOCOL: OPTIMIZATION OF LENTIVIRAL TRANSDUCTION USING SPINFECTION Last Modified: April 2018 Last Review: October 2018 PROTOCOL: OPTIMIZATION OF LENTIVIRAL TRANSDUCTION USING SPINFECTION Table of Contents 1. Brief Description 1 2. Materials and Reagents.1 3. Optimization

More information

Constitutive Reporter Lentiviral Vectors Expressing Fluorescent Proteins

Constitutive Reporter Lentiviral Vectors Expressing Fluorescent Proteins Constitutive Reporter Lentiviral Vectors Expressing Fluorescent Proteins www.vectalys.com/products/ Constitutive Reporter Lentiviral Vectors Catalog Number referring to this User Manual: 0008VCT; 0009VCT;

More information

3. RESULTS AND DISCUSSION

3. RESULTS AND DISCUSSION 3. RESULTS AND DISCUSSION 3.1. PHYTOCHEMICAL INVESTIGATIONS OF F.RACEMOSA BARK The course powder of Ficus racemosa was sequentially extracted by Pet. Ether, Ethyl acetate along with Alcohol by Soxhlet

More information

Test Report. Efficacy of A New JM Nanocomposite Material in Inhibiting Respiratory Syncytial Virus Cellular Infection

Test Report. Efficacy of A New JM Nanocomposite Material in Inhibiting Respiratory Syncytial Virus Cellular Infection Test Report Efficacy of A New JM Nanocomposite Material in Inhibiting Respiratory Syncytial Virus Cellular Infection Test Reagent New JM Nanocomposite Material Project Commissioner JM Material Technology,

More information

HIV-1 Virus-like Particle Budding Assay Nathan H Vande Burgt, Luis J Cocka * and Paul Bates

HIV-1 Virus-like Particle Budding Assay Nathan H Vande Burgt, Luis J Cocka * and Paul Bates HIV-1 Virus-like Particle Budding Assay Nathan H Vande Burgt, Luis J Cocka * and Paul Bates Department of Microbiology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, USA

More information

Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set

Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set Human Immunodeficiency Virus type 1 (HIV-1) gp120 / Glycoprotein 120 ELISA Pair Set Catalog Number : SEK11233 To achieve the best assay results, this manual must be read carefully before using this product

More information

Acute myocardial infarction (MI) on initial presentation was diagnosed if there was 20 minutes

Acute myocardial infarction (MI) on initial presentation was diagnosed if there was 20 minutes SUPPLEMENTAL MATERIAL Supplemental Methods Diagnosis for acute myocardial infarction Acute myocardial infarction (MI) on initial presentation was diagnosed if there was 20 minutes or more of chest pain

More information

VQA Control SOP Version 4.0 Roche Amplicor HIV-1 DNA Test, v August 2007

VQA Control SOP Version 4.0 Roche Amplicor HIV-1 DNA Test, v August 2007 1. PRINCIPLE 1.1. The Virology Quality Assurance (VQA) Laboratory provides external cell pellet controls for use in the validation of assays that detect HIV proviral DNA. 1.2. HIV seronegative peripheral

More information

Supplementary Material

Supplementary Material Supplementary Material Nuclear import of purified HIV-1 Integrase. Integrase remains associated to the RTC throughout the infection process until provirus integration occurs and is therefore one likely

More information

Apoptosis Mediated Cytotoxicity of Curcumin Analogues PGV-0 and PGV-1 in WiDr Cell Line

Apoptosis Mediated Cytotoxicity of Curcumin Analogues PGV-0 and PGV-1 in WiDr Cell Line Apoptosis Mediated Cytotoxicity of Curcumin Analogues PGV-0 and PGV-1 in WiDr Cell Line Endah Puji Septisetyani, Muthi Ikawati, Barinta Widaryanti and Edy Meiyanto* ) Cancer Chemoprevention Research Center,

More information

Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS

Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS ISSN 2395-3411 Available online at www.ijpacr.com 238 Research Article GALLIC ACID AND FLAVONOID ACTIVITIES OF AMARANTHUS GANGETICUS G. Jyoti Jain 1* and S. Ramachandra Setty 2 1 Department of Pharmacology,

More information

2009 H1N1 Influenza ( Swine Flu ) Hemagglutinin ELISA kit

2009 H1N1 Influenza ( Swine Flu ) Hemagglutinin ELISA kit 2009 H1N1 Influenza ( Swine Flu ) Hemagglutinin ELISA kit Catalog Number : SEK001 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as

More information

Supplementary material: Materials and suppliers

Supplementary material: Materials and suppliers Supplementary material: Materials and suppliers Electrophoresis consumables including tris-glycine, acrylamide, SDS buffer and Coomassie Brilliant Blue G-2 dye (CBB) were purchased from Ameresco (Solon,

More information

Spermicidal activity of the hexane extract of Piper longum: An in vitro study

Spermicidal activity of the hexane extract of Piper longum: An in vitro study SUPPLEMENTARY MATERIAL Spermicidal activity of the hexane extract of Piper longum: An in vitro study Abu Hasnath Md Golam Sarwar a, b, Ranjeet Kumar Nirala a, b, Mohammed Arif a, Beena Khillare b, Sonu

More information

Phosphate buffered saline (PBS) for washing the cells TE buffer (nuclease-free) ph 7.5 for use with the PrimePCR Reverse Transcription Control Assay

Phosphate buffered saline (PBS) for washing the cells TE buffer (nuclease-free) ph 7.5 for use with the PrimePCR Reverse Transcription Control Assay Catalog # Description 172-5080 SingleShot Cell Lysis Kit, 100 x 50 µl reactions 172-5081 SingleShot Cell Lysis Kit, 500 x 50 µl reactions For research purposes only. Introduction The SingleShot Cell Lysis

More information

Inhibition of Human Immunodeficiency Virus (HIV- 1) by Hedera helix L. Extracts and Phytoconstituents

Inhibition of Human Immunodeficiency Virus (HIV- 1) by Hedera helix L. Extracts and Phytoconstituents International Journal of Molecular Biology & Biochemistry. Volume 1, Number 1 (2013), pp. 1-7 International Research Publication House http://www.irphouse.com Inhibition of Human Immunodeficiency Virus

More information

~Lentivirus production~

~Lentivirus production~ ~Lentivirus production~ May 30, 2008 RNAi core R&D group member Lentivirus Production Session Lentivirus!!! Is it health threatening to lab technician? What s so good about this RNAi library? How to produce

More information

Supplementary Figure 1

Supplementary Figure 1 Supplementary Figure 1 Supplementary Figure 1: Cryopreservation alters CD62L expression by CD4 T cells. Freshly isolated (left) or cryopreserved PBMCs (right) were stained with the mix of antibodies described

More information

Phytochemical and Physico-Chemical Analysis of Siddha Preparation Magizham Pattai Chooranam

Phytochemical and Physico-Chemical Analysis of Siddha Preparation Magizham Pattai Chooranam International Journal of Research in Pharmacy and Biosciences Volume 1, Issue 2, December 2014, PP 11-15 ISSN 2394-5885 (Print) & ISSN 2394-5893 (Online) Phytochemical and Physico-Chemical Analysis of

More information

Studies on biodeterioration of some chemical constituents in fresh and market roots of drug Desmodium gangeticum DC. under storage

Studies on biodeterioration of some chemical constituents in fresh and market roots of drug Desmodium gangeticum DC. under storage Available online at www.pelagiaresearchlibrary.com European Journal of Experimental Biology, 2013, 3(1):161-165 ISSN: 2248 9215 CODEN (USA): EJEBAU Studies on biodeterioration of some chemical constituents

More information

Doctoral Degree Program in Marine Biotechnology, College of Marine Sciences, Doctoral Degree Program in Marine Biotechnology, Academia Sinica, Taipei,

Doctoral Degree Program in Marine Biotechnology, College of Marine Sciences, Doctoral Degree Program in Marine Biotechnology, Academia Sinica, Taipei, Cyclooxygenase 2 facilitates dengue virus replication and serves as a potential target for developing antiviral agents Chun-Kuang Lin 1,2, Chin-Kai Tseng 3,4, Yu-Hsuan Wu 3,4, Chih-Chuang Liaw 1,5, Chun-

More information

NICD Laboratory, South Africa

NICD Laboratory, South Africa Standard Operating Procedure for Measuring Neutralizing Antibodies against HIV-1 Pseudovirus using a Luciferase Reporter Gene Assay in TZM-bl Cells NICD Laboratory, South Africa Neutnet code:10 Contact

More information

Application Note. Introduction

Application Note. Introduction Simultaneously Measuring Oxidation of Exogenous and Endogenous Fatty Acids Using the XF Palmitate-BSA FAO Substrate with the Agilent Seahorse XF Cell Mito Stress Test Application Note Introduction The

More information

Journal of Chemical and Pharmaceutical Research

Journal of Chemical and Pharmaceutical Research Available on line www.jocpr.com Journal of Chemical and Pharmaceutical Research ISSN No: 0975-7384 CODEN(USA): JCPRC5 J. Chem. Pharm. Res., 2011, 3(4):188-195 Immunomodulatory effect of hexane extract

More information

Kulkarni GT et al. IRJP 1 (1)

Kulkarni GT et al. IRJP 1 (1) INTERNATIONAL RESEARCH JOURNAL OF PHARMACY Available online http://www.irjponline.com Research Article PHYTOCHEMICAL STUDIES AND IN VITRO CYTOTOXICITY SCREENING OF PIPER BETLE LEAF (PBL) EXTRACT Chaurasia

More information

Antiviral Efficacy of the Novel Compound BIT225 against HIV-1 Release from Human Macrophages

Antiviral Efficacy of the Novel Compound BIT225 against HIV-1 Release from Human Macrophages ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Feb. 2010, p. 835 845 Vol. 54, No. 2 0066-4804/10/$12.00 doi:10.1128/aac.01308-09 Copyright 2010, American Society for Microbiology. All Rights Reserved. Antiviral

More information

Focus Application. Compound-Induced Cytotoxicity

Focus Application. Compound-Induced Cytotoxicity xcelligence System Real-Time Cell Analyzer Focus Application Compound-Induced Cytotoxicity Featured Study: Using the Time Resolving Function of the xcelligence System to Optimize Endpoint Viability and

More information

Lentivirus Titer Determination. Speaker: Chi-long Lin May.,2008

Lentivirus Titer Determination. Speaker: Chi-long Lin May.,2008 Lentivirus Titer Determination Speaker: Chi-long Lin May.,2008 Why do we need to get virus titer? RNAi knockdown vs. Viral dose Incomplete infection Ideal RNAi KD effect Cytotoxicity / Off target 0 X Y

More information

SUPPLEMENTARY MATERIAL

SUPPLEMENTARY MATERIAL SUPPLEMENTARY MATERIAL Purification and biochemical properties of SDS-stable low molecular weight alkaline serine protease from Citrullus Colocynthis Muhammad Bashir Khan, 1,3 Hidayatullah khan, 2 Muhammad

More information

Low Cell Binding Property of LIPIDURE -COAT

Low Cell Binding Property of LIPIDURE -COAT Technical Note_1 ver.1 Low Cell Binding Property of LIPIDURE -COAT 1. LIPIDURE -COAT MULTI DISH A-6MD (Cat. No. 51011617) 2. Cell; NIH 3T3 (Fibroblast, mouse) 1. 10 %CS-DMEM; DMEM (Dulbecco's Modified

More information

Therapeutic Immunization with Autologous DC Pulsed with Autologous Inactivated HIV-1 Infected Apoptotic Cells

Therapeutic Immunization with Autologous DC Pulsed with Autologous Inactivated HIV-1 Infected Apoptotic Cells Therapeutic Immunization with Autologous DC Pulsed with Autologous Inactivated HIV-1 Infected Apoptotic Cells Sharon A. Riddler, MD, MPH University of Pittsburgh May 2008 Slide 1 HIV and DC Vaccines During

More information

VIRAL TITER COUNTS. The best methods of measuring infectious lentiviral titer

VIRAL TITER COUNTS. The best methods of measuring infectious lentiviral titer VIRAL TITER COUNTS The best methods of measuring infectious lentiviral titer FLUORESCENCE CYCLES qpcr of Viral RNA SUMMARY Viral vectors are now routinely used for gene transduction in a wide variety of

More information

Study N 286V AGRI GERM /14

Study N 286V AGRI GERM /14 Study N 286V15-2015-01 1/14 TEST REPORT VIRUCIDAL ACTIVITY OF THE PRODUCT AGAINST THE ENTERIC CYTOPATHOGENIC BOVINE ORPHAN VIRUS (ECBO) Delivered to: For : Mme FELBACQ-SERRANO Laboratoires CEETAL 1, rue

More information

Urinary 8-Isoprostane ELISA kit

Urinary 8-Isoprostane ELISA kit Urinary 8-Isoprostane ELISA kit Catalog Number: 8isoU Store at -20 C. FOR RESEARCH USE ONLY V.020420 Introduction This competitive ELISA kit is for determination of free and glucronidated 8-isoprostane

More information

IN VITRO ANTIMICROBIAL ACTIVITY OF VARIOUS EXTRACTS OF MIRABILIS JALAPA LEAVES

IN VITRO ANTIMICROBIAL ACTIVITY OF VARIOUS EXTRACTS OF MIRABILIS JALAPA LEAVES Int. J. Chem. Sci.: 8(1), 2010, 559-564 IN VITRO ANTIMICROBIAL ACTIVITY OF VARIOUS EXTRACTS OF MIRABILIS JALAPA LEAVES R. MEERA *, P. DEVI a, P. MUTHUMANI, B. KAMESWARI b and B. ESWARAPRIYA c Department

More information