IL-4 Monoclonal Antibody (11B11), Functional Grade, ebioscience Catalog Number

Transcription

1 Website: thermofisher.com/ebioscience Customer Service (US): thermofisher.com/contactus IL-4 Monoclonal Antibody (11B11), Functional Grade, ebioscience Catalog Number Details Size 50 µg Host/Isotope Class Type Clone Conjugate Form Concentration Purification Rat / IgG1, kappa Monoclonal Antibody 11B11 Functional Grade Liquid 1 mg/ml Affinity chromatography Storage buffer PBS, ph 7.2 Contains Storage Conditions no preservative 4 C Product data sheet Species Reactivity Tested species reactivity Mouse Published species reactivity Mouse Tested Applications Dilution * ELISA (ELISA) µg/ml Functional Assay (FN) Assay-Dependent Published Applications Flow Cytometry (Flow) See 6 publications below ELISA (ELISA) See 2 publications below Miscellaneous PubMed (MISC) See 2 publications below Immunofluorescence (IF) See 1 publications below Functional Assay (FN) See 2 publications below * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls. Product specific information Description: The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kd cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is speciesspecific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23. Applications Reported: This 11B11 antibody has been reported for use in cytokine neutralization, ELISPOT, and ELISA cap. Applications Tested: The Functional Grade Purified 11B11 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-4 bioactivity. The 11B11 antibody at µg/ml has been found to inhibit by 50% the biological effects of 1.0 ng/ml mouse IL-4 in a CTLL-2 cell proliferation assay. Please refer to Best Protocols for more information. The 11B11 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-4 (IL-4) in combination with the biotinylated BVD6-24G2 (cat ) antibody for detection and recombinant mouse IL-4 (cat ) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/ml - 4 pg/ml should be included in each ELISA plate. Endotoxin: Less than ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 µm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than ng/µg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

2 Background/Target Information The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

3 Product Images For IL-4 Monoclonal Antibody (11B11), Functional Grade, ebioscience IL-4 Antibody ( ) in ELISA Splenocytes from 8 week old mice pre-immunized with OVA/IFA were recallactivated with OVA antigen for 36 hours in Mouse IL-4 ELISPOT assay. Control well is medium alone.

4 PubMed References For IL-4 Monoclonal Antibody (11B11), Functional Grade, ebioscience 6 Flow Cytometry References was used in Flow cytometry/cell sorting to investigate the effect Paeoniflorin has on Th cells and dendritic cells in a mouse MS model. 2 ELISA References Scientific reports (Feb 2017; 7: null) "Paeoniflorin Ameliorates Experimental Autoimmune Encephalomyelitis via Inhibition of Dendritic Cell Function and Th17 Cell Differentiation." Author(s):Zhang H,Qi Y,Yuan Y,Cai L,Xu H,Zhang L,Su B,Nie H PubMed Article URL: was used in Flow cytometry/cell sorting to define mechanisms regulating nth17 cell development and reveal the roles of Akt and mtor in shaping subsets of T cells. Nature immunology (Jun 2013; 14: 611) "Natural and inducible TH17 cells are regulated differently by Akt and mtor pathways." Author(s):Kim JS,Sklarz T,Banks LB,Gohil M,Waickman AT,Skuli N,Krock BL,Luo CT,Hu W,Pollizzi KN,Li MO,Rathmell JC, Birnbaum MJ,Powell JD,Jordan MS,Koretzky GA PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the relationships between recent thymic emigrants and T- helper type 1 and 2 cells. Blood (Jan 2011; 117: 1239) "Recent thymic emigrants are biased against the T-helper type 1 and toward the T-helper type 2 effector lineage." Author(s):Hendricks DW,Fink PJ PubMed Article URL: was used in Flow cytometry/cell sorting to investigate the impact of eliciting T-cell help from activated inkt cells using alpha-galactosylceramide, showing potent anti-tumour response. Immunology and cell biology (Jul 2010; 88: 596) "Potent anti-tumor responses to immunization with dendritic cells loaded with tumor tissue and an NKT cell ligand." Author(s):Petersen TR,Sika-Paotonu D,Knight DA,Dickgreber N,Farrand KJ,Ronchese F,Hermans IF PubMed Article URL: was used in Flow cytometry/cell sorting to determine that ADAM8 plays a crucial proinflammatory role in airway inflammation. American journal of respiratory and critical care medicine (Jun 2010; 181: 1318) "The metalloprotease-disintegrin ADAM8 is essential for the development of experimental asthma." Author(s):Naus S,Blanchet MR,Gossens K,Zaph C,Bartsch JW,McNagny KM,Ziltener HJ PubMed Article URL: was used in Flow cytometry/cell sorting to study the effect of STAT6 delection on Lat(Y136F) mice. Journal of immunology (Baltimore, Md. : 1950) (Mar 2009; 182: 2680) "STAT6 deletion converts the Th2 inflammatory pathology afflicting Lat(Y136F) mice into a lymphoproliferative disorder involving Th1 and CD8 effector T cells." Author(s):Archambaud C,Sansoni A,Mingueneau M,Devilard E,Delsol G,Malissen B,Malissen M PubMed Article URL: was used in an ELISpot assay to develop a recombinant adenovirus Ad-EVVLP for the treatment of hand, foot and mouth disease, providing a possible vaccine for future immunisation against the disease. PLoS neglected tropical diseases (Apr 2015; 9: null) "Recombinant adeno-vaccine expressing enterovirus 71-like particles against hand, foot, and mouth disease." Author(s):Tsou YL,Lin YW,Shao HY,Yu SL,Wu SR,Lin HY,Liu CC,Huang C,Chong P,Chow YH PubMed Article URL:

5 2 Miscellaneous PubMed References "Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and " 1 Immunofluorescence References 2 Functional Assay References was used in an ELISpot assay to develop a recombinant adenovirus Ad-EVVLP for the treatment of hand, foot and mouth disease, providing a possible vaccine for future immunisation against the disease. PLoS neglected tropical diseases (Apr 2015; 9: null) "Recombinant adeno-vaccine expressing enterovirus 71-like particles against hand, foot, and mouth disease." Author(s):Tsou YL,Lin YW,Shao HY,Yu SL,Wu SR,Lin HY,Liu CC,Huang C,Chong P,Chow YH PubMed Article URL: "Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and " was used in Immunofluorescence to demonstrate the temporally and functionally distinct roles for B cells in regulating CD4 T cell responses. Journal of immunology (Baltimore, Md. : 1950) (Dec 2013; 191: 6052) "Temporal requirements for B cells in the establishment of CD4 T cell memory." Author(s):Mollo SB,Zajac AJ,Harrington LE PubMed Article URL: "Trpm4 differentially regulates Th1 and Th2 function by altering calcium signaling and " was used in Functional assays to investigate Foxp3 as the master switch for Tregs, showing that expression is regulated by DNA methylation in Tregs. Journal of immunology (Baltimore, Md. : 1950) (Jan 2009; 182: 259) "Epigenetic regulation of Foxp3 expression in regulatory T cells by DNA methylation." Author(s):Lal G,Zhang N,van der Touw W,Ding Y,Ju W,Bottinger EP,Reid SP,Levy DE,Bromberg JS PubMed Article URL: