JAK3 inhibitor administration in vivo in chronically SIV Infected Rhesus Macaques
|
|
- Arlene Moody
- 6 years ago
- Views:
Transcription
1 JAK3 inhibitor administration in vivo in chronically SIV Infected Rhesus Macaques preferentially depletes NK Cells Ladawan Kowawisatsut 1 Kovit Pattanapanyasat 1 Aftab A. Ansari 2 1 Department of Immunology Faculty of Medicine Siriraj Hospital Mahidol University Thailand 1 Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand 2 Department of Pathology and Experimental Medicine, Emory University, USA
2 Why study NK-17 cells in the GALT? GALT is the one of the largest lymphocyte y storage site and also the primary site of HIV/SIV replication during acute infection NK cells are important innate cells in anti-viral immunity NK cells in Toxoplasmagondii-infected mice can produce IL-17 in the presence of IL-6, IL-23 and TGF- (The Journal of Immunology 2010,184: ) 184: ) IL-17 producing Th cells (Th17) have been reported as the important cells in protection of intestinal epithelial integrity and microbial translocation during SIV infection
3 JAK3 inhibitor Inhibits the common gamma chain cytokine pathways such as the IL-2, IL-7 and IL-15 signaling pathway Immunosuppressive agents that has been used in the clinic for the prevention of organ transplant rejection and autoimmune diseases Oral administration of the JAK3 inhibitor in cynomolgus monkey reduced the number of NK cells and CD8+ T cell which returned to baseline levels within 3 weeks following treatment cessation (Journal of Leukocyte Biology 2004;76: )
4 Pharmacokinetics of JAK3 inhibitor Groups of two rhesus macaques were administered 1, 5, 10, or 25 mg/kg JAK3 inhibitor orally and detailed safety and PK studies performed. At a dose of 10 mg/kg/day, sufficient drug trough levels were obtained that led to the preferential depletion of NK cells Dose was tolerated well with no clinical, metabolic, hematologic, renal/liver toxicity.
5 Aim of the study To determine the effects of in vivo NK cell depletion on viral loads and immune responses using a JAK3 inhibitor in chronically SIV infected rhesus macaques
6 Protocol Blood and Rectal biopsy from 6 intravenously SIVmac251- infected Rhesus macaques JAK3 inhibitor 10 mg/kg/day g for 35 days per os pre weeks Peripheral blood & colorectal biopsy collection to determine frequency of total and IL-17 NK cells Plasma monitored for drug levels PBMC analyzed for levels of phosphorylated STAT-5
7 Methods Mononuclear cells from blood and rectal biopsy py Stimulate with 25 ng/ml PMA and 1 mg/ml Ionomycin Add 0.5 mg/ml Brefeldin A Overnight stimulation Cell surface and Intracellular cytokine staining FITC PE PerCP-Cy5 Cy5.55 PE-Cy7 APC Alexa700 PacBlue CD8 NKG2A CD16 CD56 IL-17 CD3 Live/Dead Flow cytometry analysis by LSRII
8 JAK3 inhibitor had modest effects on the frequencies of CD4 and CD8 T cells in PBMC and rectal biopsies * JAK 3 inhibitor CD4 T cells JAK 3 inhibitor CD8 T cells
9 JAK-3 inhibitor depleted NK cells in PBMC and rectal biopsy * JAK 3 inhibitor After cessation NK cells returned in PBMC, but not in rectal biopsy
10 JAK3 inhibitor also effects NK-17 cells in PBMC and rectal biopsy * * JAK 3 inhibitor PBMC JAK 3 inhibitor Rectal biopsy
11 Viral Loads in SIVmac251 chronically infected Rhesus Macaques administered JAK3 inhibitor copies/ml JAK 3 inhibitor Transient increase in viremia during JAK-3 inhibitor admin.
12 Viral Loads in SIVmac251 chronically infected Rhesus Macaques administered JAK3 inhibitor copies/ml JAK 3 inhibitor Transient increase of viremia during JAK3 inhibitor admin.
13 Conclusion The in vivo administration of a JAK3 inhibitor to chronically SIVmac251 infected monkeys depletes both total and NK-17 cells in the blood and mucosal tissue and is associated with a transient increases in viremia denoting an important role for NK cells during chronic SIV infection.
14 Acknowledgement Department of Pathology and Experimental Medicine Emory University School of Medicine Professor Aftab AA A.Ansari Ann Mayne Yoshi Takahashi Dawn Little Yerkes National Primate Research Center Emory University Department of Immunology, Faculty of Medicine Siriraj Hospital, Mahidol University Professor Kovit Pattanapanyasat The Royal Golden Jubilee Ph.D. Program, The Thailand Research Fund Grant from NIAID, NIH to Prof. A. Ansari
15 * * Mean ±SD PBMC Rectum Pre CP 4.80± ±0.88 Week ± ±4.98 Week ± ±1.19 Week ± ±0.62 Week ± ±0.64 Week ± ±0.70
In Vivo Administration of a JAK3 Inhibitor during Acute SIV Infection Leads to Significant Increases in Viral Load during Chronic Infection
In Vivo Administration of a JAK3 Inhibitor during Acute SIV Infection Leads to Significant Increases in Viral Load during Chronic Infection Yoshiaki Takahashi, Emory University Siddappa N. Byrareddy, Emory
More informationFinal published version:
In Vivo Administration of a JAK3 Inhibitor to Chronically SIV Infected Rhesus Macaques Leads to NK Cell Depletion Associated with Transient Modest Increase in Viral Loads Yoshiaki Takahashi, Emory University
More informationCombined IL-21 and IFNα treatment limits inflammation and delay viral rebound in ART-treated, SIV-infected macaques
Combined and IFNα treatment limits inflammation and delay viral rebound in ART-treated, SIV-infected macaques Mirko Paiardini Associate Professor, Emory University School of Medicine Yerkes National Primate
More informationOptimizing Intracellular Flow Cytometry
Optimizing Intracellular Flow Cytometry Detection of Cytokines, Transcription Factors, and Phosphoprotein by Flow Cytometry Presented by Erika O Donnell, PhD, BD Biosciences 23-14876-00 Outline Basic principles
More informationLoss of Effector and Anti-Inflammatory Natural Killer T Lymphocyte Function in Pathogenic Simian Immunodeficiency Virus Infection
Loss of Effector and Anti-Inflammatory Natural Killer T Lymphocyte Function in Pathogenic Simian Immunodeficiency Virus Infection Namita Rout, Harvard University Justin Greene, University of Wisconsin-Madison
More informationOptimizing Intracellular Flow Cytometry:
Optimizing Intracellular Flow Cytometry: Simultaneous Detection of Cytokines and Transcription Factors An encore presentation by Jurg Rohrer, PhD, BD Biosciences 10.26.10 Outline Introduction Cytokines
More informationDavid Verhoeven, Sumathi Sankaran, Melanie Silvey, and Satya Dandekar*
JOURNAL OF VIROLOGY, Apr. 2008, p. 4016 4027 Vol. 82, No. 8 0022-538X/08/$08.00 0 doi:10.1128/jvi.02164-07 Copyright 2008, American Society for Microbiology. All Rights Reserved. Antiviral Therapy during
More informationPaucity of CD4+ Natural Killer T (NKT) Lymphocytes in Sooty Mangabeys is Associated with Lack of NKT Cell Depletion After SIV Infection
Paucity of CD4+ Natural Killer T (NKT) Lymphocytes in Sooty Mangabeys is Associated with Lack of NKT Cell Depletion After SIV Infection The Harvard community has made this article openly available. Please
More informationCD4 T Cells, Including Th17 and Cycling Subsets, Are Intact in the Gut Mucosa of HIV-1-Infected Long-Term Nonprogressors
JOURNAL OF VIROLOGY, June 2011, p. 5880 5888 Vol. 85, No. 12 0022-538X/11/$12.00 doi:10.1128/jvi.02643-10 Copyright 2011, American Society for Microbiology. All Rights Reserved. CD4 T Cells, Including
More informationOptimizing Intracellular Flow Cytometry:
Optimizing Intracellular Flow Cytometry: Simultaneous Detection of Cytokines and Transcription Factors Presented by Jurg Rohrer, PhD, BD Biosciences 23-10780-00 Outline Introduction Cytokines Transcription
More informationAvailability of activated CD4+ T cells dictates the level of viremia in naturally SIV-infected sooty mangabeys
Availability of activated CD4+ T cells dictates the level of viremia in naturally SIV-infected sooty mangabeys Nichole R. Klatt, Emory University Francois Villinger, Emory University Pavel Bostik, Emory
More informationPeptide stimulation and Intracellular Cytokine Staining
v Peptide stimulation and Intracellular Cytokine Staining Authors A. Cosma, S. Allgayer MATERIALS Date 01-02-2007 REAGENTS: Version 1.0 - PBMCs - Culture medium - Costimulating antibodies - Peptide pools
More informationCover Page. The handle holds various files of this Leiden University dissertation.
Cover Page The handle http://hdl.handle.net/1887/23854 holds various files of this Leiden University dissertation. Author: Marel, Sander van der Title: Gene and cell therapy based treatment strategies
More informationNKTR-255: Accessing The Immunotherapeutic Potential Of IL-15 for NK Cell Therapies
NKTR-255: Accessing The Immunotherapeutic Potential Of IL-15 for NK Cell Therapies Saul Kivimäe Senior Scientist, Research Biology Nektar Therapeutics NK Cell-Based Cancer Immunotherapy, September 26-27,
More informationChallenges of Integrating Mucosal Immune Assays into HIV Vaccine Trials KAVI
Challenges of Integrating Mucosal Immune Assays into HIV Vaccine Trials Omu Anzala, MBChB, PhD KAVI University of Nairobi Outline Why mucosal immunology Experience from Kenya Challenges The way forward
More informationJanuary 25, 2017 Scientific Research Process Name of Journal: ESPS Manuscript NO: Manuscript Type: Title: Authors: Correspondence to
January 25, 2017 Scientific Research Process Name of Journal: World Journal of Gastroenterology ESPS Manuscript NO: 31928 Manuscript Type: ORIGINAL ARTICLE Title: Thiopurine use associated with reduced
More informationTarget Cell Availability, Rather than Breast Milk Factors, Dictates Mother-to-Infant Transmission of SIV in Sooty Mangabeys and Rhesus Macaques
Target Cell Availability, Rather than Breast Milk Factors, Dictates Mother-to-Infant Transmission of SIV in Sooty Mangabeys and Rhesus Macaques Ann Chahroudi, Emory University Emily Cartwright, Emory University
More informationMATERIALS. Peptide stimulation and Intracellular Cytokine Staining- EFFECTOR 45RA PANEL
v Peptide stimulation and Intracellular Cytokine Staining- EFFECTOR 45RA PANEL Authors S. Kutscher, A. Cosma, MATERIALS REAGENTS: - PBMCs - Culture medium - Costimulating antibodies - Peptide pools including
More informationNKTR-255: Accessing IL-15 Therapeutic Potential through Robust and Sustained Engagement of Innate and Adaptive Immunity
NKTR-255: Accessing IL-15 Therapeutic Potential through Robust and Sustained Engagement of Innate and Adaptive Immunity Peiwen Kuo Scientist, Research Biology Nektar Therapeutics August 31 st, 2018 Emerging
More informationInterleukin-21 combined with ART reduces inflammation and viral reservoir in SIV-infected macaques
Interleukin-21 combined with ART reduces inflammation and viral reservoir in SIV-infected macaques Luca Micci, Emory University Emily S. Ryan, Emory University Rémi Fromentin, Université de Montréal Steven
More informationDEBATE ON HIV ENVELOPE AS A T CELL IMMUNOGEN HAS BEEN GAG-GED
DEBATE ON HIV ENVELOPE AS A T CELL IMMUNOGEN HAS BEEN GAG-GED Viv Peut Kent Laboratory, University of Melbourne, Australia WHY ENVELOPE? Env subject to both humoral and cellular immune responses Perhaps
More informationViral Reservoirs and anti-latency interventions in nonhuman primate models of SIV/SHIV infection
Viral Reservoirs and anti-latency interventions in nonhuman primate models of SIV/SHIV infection Koen Van Rompay California National Primate Research Center University of California Davis Outline Introduction
More informationExperimental SIV infection of rhesus macaques (RMs),
Depletion of CD8 Cells in Sooty Mangabey Monkeys Naturally Infected with Simian Immunodeficiency Virus Reveals Limited Role for Immune Control of Virus Replication in a Natural Host Species 1 Ashley P.
More informationPharmacokinetic and pharmacodynamic profile of maraviroc in rhesus macaques after a single oral dose
HIV Transmission Workshop 011 Pharmacokinetic and pharmacodynamic profile of maraviroc in rhesus macaques after a single oral dose Wutyi Aung, Amy Martin, Mian-er Cong, Jessica Radzio, Elizabeth Sweeney,
More informationEarly treatment of SIV plus macaques with an alpha(4)beta(7) mab alters virus distribution and preserves CD4(+) T cells in later stages of infection
Early treatment of SIV plus macaques with an alpha(4)beta(7) mab alters virus distribution and preserves CD4(+) T cells in later stages of infection Philip J. Santangelo, Emory University Claudia Cicala,
More informationCover Page. The handle holds various files of this Leiden University dissertation
Cover Page The handle http://hdl.handle.net/1887/24366 holds various files of this Leiden University dissertation Author: Buddingh, Emilie Pauline Title: Innate immunity in osteosarcoma Issue Date: 2014-03-05
More informationEBV Infection and Immunity. Andrew Hislop Institute for Cancer Studies University of Birmingham
EBV Infection and Immunity Andrew Hislop Institute for Cancer Studies University of Birmingham EBV Introduction Large ds DNA virus Spread by saliva contact Lifelong infection Predominantly B-lymphotropic
More informationEffector mechanisms of cell-mediated immunity: Properties of effector, memory and regulatory T cells
ICI Basic Immunology course Effector mechanisms of cell-mediated immunity: Properties of effector, memory and regulatory T cells Abul K. Abbas, MD UCSF Stages in the development of T cell responses: induction
More informationVaccine-induced myeloid cell population dampens protective immunity to SIV
Research article Related Commentary, page 2364 Vaccine-induced myeloid cell population dampens protective immunity to SIV Yongjun Sui, 1 Alison Hogg, 1 Yichuan Wang, 1 Blake Frey, 1 Huifeng Yu, 1 Zheng
More informationT Cell Activation, Costimulation and Regulation
1 T Cell Activation, Costimulation and Regulation Abul K. Abbas, MD University of California San Francisco 2 Lecture outline T cell antigen recognition and activation Costimulation, the B7:CD28 family
More informationReplicating measles-shiv vaccine induces long term preservation of central memory CD4 cells in the gut of macaques challenged with SHIV89.
Replicating measles-shiv vaccine induces long term preservation of central memory CD4 cells in the gut of macaques challenged with SHIV89.6P Frédéric Tangy Viral Genomics and Vaccination Laboratory Measles
More informationankylosing spondylitis Department of Clinical Immunology, Xijing Hospital, The Fourth Military
Functional defects in CD4 + CD25 high FoxP3 + regulatory cells in ankylosing spondylitis Huifang Guo 1, 2, 3, Ming Zheng 1, 2, 3, Kui Zhang 1, 3, Fengfan Yang 1, 3, Xin Zhang 1, 3, Qing Han 1, 3, Zhi-Nan
More informationVaccine Delivery and TLR Ligands Influence the Quality of T cell Responses in NHP. Robert A. Seder, M.D. Vaccine Research Center NIAID, NIH
Vaccine Delivery and TLR Ligands Influence the Quality of T cell Responses in NHP Robert A. Seder, M.D. Vaccine Research Center NIAID, NIH Vaccines Against HIV, Malaria and Tuberculosis Will Require Neutralizing
More informationAntigen Presentation and T Lymphocyte Activation. Abul K. Abbas UCSF. FOCiS
1 Antigen Presentation and T Lymphocyte Activation Abul K. Abbas UCSF FOCiS 2 Lecture outline Dendritic cells and antigen presentation The role of the MHC T cell activation Costimulation, the B7:CD28 family
More informationAnimal Models to Understand Immunity
Animal Models to Understand Immunity Hussein El Saghire hesaghir@sckcen.be Innate Adaptive immunity Immunity MAPK and NF-kB TLR pathways receptors Fast Slow Non-specific Specific NOD-like receptors T-cell
More informationChapter 11 CYTOKINES
Chapter 11 CYTOKINES group of low molecular weight regulatory proteins secreted by leukocytes as well as a variety of other cells in the body (8~30kD) regulate the intensity and duration of the immune
More informationSupporting Information
Supporting Information Sui et al..7/pnas.997 Pre-CLP CM9 LA9 SL Tat# Pol Vif % Tetramer + CD + CD + Vac+IL- +IL- Vac Fig. S. Frequencies of six different CD + CD + Mamu-A*-tetramer + cells were measured
More informationAntibody-Dependent Cell-Mediated Cytotoxicity in Simian Immunodeficiency Virus-Infected Rhesus Monkeys
JOURNAL OF VIROLOGY, July 2011, p. 6906 6912 Vol. 85, No. 14 0022-538X/11/$12.00 doi:10.1128/jvi.00326-11 Copyright 2011, American Society for Microbiology. All Rights Reserved. Antibody-Dependent Cell-Mediated
More informationRelationship of menstrual cycle and vaginal infection in female rhesus macaques challenged with repeated, low doses of SIVmac251
Relationship of menstrual cycle and vaginal infection in female rhesus macaques challenged with repeated, low doses of SIVmac251 Monica Morris, Total Solutions Siddappa Byrareddy, Emory University Francois
More informationMicrobial exposure alters HIV-1-induced mucosal CD4 + T cell death pathways Ex vivo
Steele et al. Retrovirology 2014, 11:14 RESEARCH Open Access Microbial exposure alters HIV-1-induced mucosal CD4 + T cell death pathways Ex vivo Amanda K Steele 1, Eric J Lee 1, Jennifer A Manuzak 1,2,
More informationReceived 4 December 2001/Accepted 29 April 2002
JOURNAL OF VIROLOGY, Aug. 2002, p. 8433 8445 Vol. 76, No. 16 0022-538X/02/$04.00 0 DOI: 10.1128/JVI.76.16.8433 8445.2002 Copyright 2002, American Society for Microbiology. All Rights Reserved. The Relationship
More informationSupplementary Figures
Inhibition of Pulmonary Anti Bacterial Defense by IFN γ During Recovery from Influenza Infection By Keer Sun and Dennis W. Metzger Supplementary Figures d a Ly6G Percentage survival f 1 75 5 1 25 1 5 1
More informationPD-1 blockade during chronic SIV infection reduces hyperimmune activation and microbial translocation in rhesus macaques
Brief report Related Commentary, page 1611 PD-1 blockade during chronic SIV infection reduces hyperimmune activation and microbial translocation in rhesus macaques Ravi Dyavar Shetty, 1 Vijayakumar Velu,
More informationT Lymphocyte Activation and Costimulation. FOCiS. Lecture outline
1 T Lymphocyte Activation and Costimulation Abul K. Abbas, MD UCSF FOCiS 2 Lecture outline T cell activation Costimulation, the B7:CD28 family Inhibitory receptors of T cells Targeting costimulators for
More informationPreferential depletion of gut CD4-expressing inkt cells contributes to systemic immune activation in HIV-1 infection
nature publishing group ARTICLES Preferential depletion of gut CD4-expressing inkt cells contributes to systemic immune activation in HIV-1 infection FJ Ibarrondo 1, SB Wilson 2, LE Hultin 1, R Shih 1,
More informationSupplementary Figure S1. Flow cytometric analysis of the expression of Thy1 in NH cells. Flow cytometric analysis of the expression of T1/ST2 and
Supplementary Figure S1. Flow cytometric analysis of the expression of Thy1 in NH cells. Flow cytometric analysis of the expression of T1/ST2 and Thy1 in NH cells derived from the lungs of naïve mice.
More informationSupplementary Figure 1. ETBF activate Stat3 in B6 and Min mice colons
Supplementary Figure 1 ETBF activate Stat3 in B6 and Min mice colons a pstat3 controls Pos Neg ETBF 1 2 3 4 b pstat1 pstat2 pstat3 pstat4 pstat5 pstat6 Actin Figure Legend: (a) ETBF induce predominantly
More informationHuman Innate Lymphoid Cells: crosstalk with CD4 + regulatory T cells and role in Type 1 Diabetes
Joint Lab Meeting 03/03/2015 Human Innate Lymphoid Cells: crosstalk with CD4 + regulatory T cells and role in Type 1 Diabetes Caroline Raffin Bluestone Lab ILCs: Definition - Common lymphoid progenitor
More informationRe-Evaluation of PD-1 Expression by T Cells as a Marker for Immune Exhaustion during SIV Infection
Re-Evaluation of PD-1 Expression by T Cells as a Marker for Immune Exhaustion during SIV Infection Jung Joo Hong, Emory University Praveen K Amancha, Emory University Kenneth Rogers, Emory University Aftab
More informationInfectious SIV resides in adipose tissue and induces metabolic defects in chronically infected rhesus macaques
Couturier et al. Retrovirology (216) 13:3 DOI 1.1186/s12977-16-26-2 Retrovirology RESEARCH Open Access Infectious SIV resides in adipose tissue and induces metabolic defects in chronically infected rhesus
More informationTable S1. Viral load and CD4 count of HIV-infected patient population
Table S1. Viral load and CD4 count of HIV-infected patient population Subject ID Viral load (No. of copies per ml of plasma) CD4 count (No. of cells/µl of blood) 28 7, 14 29 7, 23 21 361,99 94 217 7, 11
More informationInterferon γ regulates idiopathic pneumonia syndrome, a. Th17 + CD4 + T-cell-mediated GvH disease
Interferon γ regulates idiopathic pneumonia syndrome, a Th17 + CD4 + T-cell-mediated GvH disease Nora Mauermann, Julia Burian, Christophe von Garnier, Stefan Dirnhofer, Davide Germano, Christine Schuett,
More informationSupplementary Table 1: Summary of Reactogenicity Events by Group and Time of Onset in Entebbe
Supplementary Table 1: Summary of Reactogenicity Events by Group and Time of Onset in Group A (n=39) B (n=11) A (n=39) B (n=11) Onset Post-Vaccination Event 3 Minutes 3-14 days Pain 5 (13%) 1 (1%) 6 (15%)
More informationChapter 10 (pages ): Differentiation and Functions of CD4+ Effector T Cells Prepared by Kristen Dazy, MD, Scripps Clinic Medical Group
FIT Board Review Corner September 2015 Welcome to the FIT Board Review Corner, prepared by Andrew Nickels, MD, and Sarah Spriet, DO, senior and junior representatives of ACAAI's Fellows-In-Training (FITs)
More informationEx vivo Human Antigen-specific T Cell Proliferation and Degranulation Willemijn Hobo 1, Wieger Norde 1 and Harry Dolstra 2*
Ex vivo Human Antigen-specific T Cell Proliferation and Degranulation Willemijn Hobo 1, Wieger Norde 1 and Harry Dolstra 2* 1 Department of Laboratory Medicine - Laboratory of Hematology, Radboud University
More informationIncreased Loss of CCR5 CD45RA CD4 T Cells in CD8 Lymphocyte-Depleted Simian Immunodeficiency Virus-Infected Rhesus Monkeys
JOURNAL OF VIROLOGY, June 2008, p. 5618 5630 Vol. 82, No. 11 0022-538X/08/$08.00 0 doi:10.1128/jvi.02748-07 Copyright 2008, American Society for Microbiology. All Rights Reserved. Increased Loss of CCR5
More informationEvaluation of the QuantiFERON- CMV assay as a monitoring tool in solid organ and allogenic stem cell transplantation
Evaluation of the QuantiFERON- CMV assay as a monitoring tool in solid organ and allogenic stem cell transplantation Dr Grace Thompson A/Prof Mina John CMV in transplantation Major cause of morbidity and
More informationSUPPORTING INFORMATIONS
SUPPORTING INFORMATIONS Mice MT/ret RetCD3ε KO α-cd25 treated MT/ret Age 1 month 3 mnths 6 months 1 month 3 months 6 months 1 month 3 months 6 months 2/87 Survival 87/87 incidence of 17/87 1 ary tumor
More informationSupplementary Data. Treg phenotype
Supplementary Data Additional Experiment An additional experiment was performed using cryopreserved peripheral blood mononuclear cells (PBMC) derived from five renal cell carcinoma (RCC) patients [see
More informationDiscordant CD38 measurement of CD8+ T lymphocytes using fluorescein conjugates in comparison with phycoerythrin conjugates
Original article Discordant CD38 measurement of CD8+ T lymphocytes using fluorescein conjugates in comparison with phycoerythrin conjugates Nattawat Onlamoon 1, Sutchana Tabprasit 2, Kasama Sukapirom 1,
More informationDEFINING DENDRITIC CELLS AND MACROPHAGES IN LYMPH NODES AND GUT MUCOSA DURING SIMIAN IMMUNODEFICIENCY VIRUS INFECTION. Zachary Duane Jameson Swan
DEFINING DENDRITIC CELLS AND MACROPHAGES IN LYMPH NODES AND GUT MUCOSA DURING SIMIAN IMMUNODEFICIENCY VIRUS INFECTION by Zachary Duane Jameson Swan B.S., University of North Carolina at Chapel Hill, 2006
More informationFunctional Cure of SIVagm Infection in Rhesus Macaques Results in Complete Recovery of CD4 + T Cells and Is Reverted by CD8 + Cell Depletion
Functional Cure of SIVagm Infection in Rhesus Macaques Results in Complete Recovery of CD4 + T Cells and Is Reverted by CD8 + Cell Depletion Ivona Pandrea 1,2,3, Thaidra Gaufin 4, Rajeev Gautam 4, Jan
More informationMonocyte subsets in health and disease. Marion Frankenberger
Monocyte subsets in health and disease Marion Frankenberger main cellular components: Leukocytes Erythrocytes Composition of whole blood Monocytes belong to the cellular components of peripheral blood
More informationTreatment with IL-7 Prevents the Decline of Circulating CD4 + T Cells during the Acute Phase of SIV Infection in Rhesus Macaques
SUPPORTING INFORMATION FOR: Treatment with IL-7 Prevents the Decline of Circulating CD4 + T Cells during the Acute Phase of SIV Infection in Rhesus Macaques Lia Vassena, 1,2 Huiyi Miao, 1 Raffaello Cimbro,
More informationApplication Information Bulletin: Human NK Cells Phenotypic characterizing of human Natural Killer (NK) cell populations in peripheral blood
Application Information Bulletin: Human NK Cells Phenotypic characterizing of human Natural Killer (NK) cell populations in peripheral blood Christopher A Fraker, Ph.D., University of Miami - Miami, Florida
More informationCytokines modulate the functional activities of individual cells and tissues both under normal and pathologic conditions Interleukins,
Cytokines http://highered.mcgraw-hill.com/sites/0072507470/student_view0/chapter22/animation the_immune_response.html Cytokines modulate the functional activities of individual cells and tissues both under
More informationPrimary Adult Naïve CD4+ CD45RA+ Cells. Prepared by: David Randolph at University of Alabama, Birmingham
Primary Adult Naïve CD4+ CD45RA+ Cells Prepared by: David Randolph (drdrdr@uab.edu) at University of Alabama, Birmingham Goal: To obtain large numbers of highly pure primary CD4+ CD45RO- CD25- cells from
More informationCROI 2016 Review: Immunology and Vaccines
Frontier AIDS Education and Training Center CROI 2016 Review: Immunology and Vaccines Meena Ramchandani MD MPH Acting Instructor, University of Washington March 2016 This presentation is intended for educational
More informationNIH Public Access Author Manuscript Nature. Author manuscript; available in PMC 2009 July 1.
NIH Public Access Author Manuscript Published in final edited form as: Nature. 2009 January 1; 457(7225): 87 91. doi:10.1038/nature07469. Immune Control of an SIV Challenge by a T Cell-Based Vaccine in
More informationRecommended Protocols for Phospho Protein Detection in Human Cells
Recommended Protocols for Phospho Protein Detection in Human Cells Depending on subcellular localization of the phospho protein of interest, as well as epitope susceptibility to cell fixing and permeabilizing
More informationAntibody Dependent Cellular Cytotxic activity: Past and Future. Guido Ferrari, M.D. Duke University Medical Center
Antibody Dependent Cellular Cytotxic activity: Past and Future Guido Ferrari, M.D. Duke University Medical Center Mechanism of Antibody Dependent Cellular Cytotoxicity (ADCC) ADCC Effector Cells (NK, monocytes/macrophages,
More informationSupplemental Information. T Cells Enhance Autoimmunity by Restraining Regulatory T Cell Responses via an Interleukin-23-Dependent Mechanism
Immunity, Volume 33 Supplemental Information T Cells Enhance Autoimmunity by Restraining Regulatory T Cell Responses via an Interleukin-23-Dependent Mechanism Franziska Petermann, Veit Rothhammer, Malte
More informationHLA and Non-HLA Antibodies in Transplantation and their Management
HLA and Non-HLA Antibodies in Transplantation and their Management Luca Dello Strologo October 29 th, 2016 Hystory I 1960 donor specific antibodies (DSA): first suggestion for a possible role in deteriorating
More informationPrimate models of SIV infection: Differenct susceptibility of African green monkeys and macaques to develop AIDS
Les Pensières Animal Models, 2011 Primate models of SIV infection: Differenct susceptibility of African green monkeys and macaques to develop AIDS Michaela Müller-Trutwin Unité de Régulation des Infections
More informationPhase 1 Human Trials of Rectal Microbicides
Phase 1 Human Trials of Rectal Microbicides UC781: Completed Tenofovir: Planned to start an NIH IPCP for Topical Microbicides RM Trials under U19-IPCP grant UC781 (topical) rectal trial completed A Phase
More informationSupplemental Figure 1. Signature gene expression in in vitro differentiated Th0, Th1, Th2, Th17 and Treg cells. (A) Naïve CD4 + T cells were cultured
Supplemental Figure 1. Signature gene expression in in vitro differentiated Th0, Th1, Th2, Th17 and Treg cells. (A) Naïve CD4 + T cells were cultured under Th0, Th1, Th2, Th17, and Treg conditions. mrna
More informationMedical Virology Immunology. Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University
Medical Virology Immunology Dr. Sameer Naji, MB, BCh, PhD (UK) Head of Basic Medical Sciences Dept. Faculty of Medicine The Hashemite University Human blood cells Phases of immune responses Microbe Naïve
More informationImpaired T Lymphocyte Responses in Older Macaques: Possible Implications for Lentiviral Disease Progression
University of Wisconsin Milwaukee UWM Digital Commons Theses and Dissertations May 2015 Impaired T Lymphocyte Responses in Older Macaques: Possible Implications for Lentiviral Disease Progression Sopitsuda
More informationCYTOKINE RECEPTORS AND SIGNAL TRANSDUCTION
CYTOKINE RECEPTORS AND SIGNAL TRANSDUCTION What is Cytokine? Secreted popypeptide (protein) involved in cell-to-cell signaling. Acts in paracrine or autocrine fashion through specific cellular receptors.
More informationSevere Depletion of Mucosal CD4 + T Cells in AIDS-Free Simian Immunodeficiency Virus-Infected Sooty Mangabeys
This information is current as of September 1, 2018. References Subscription Permissions Email Alerts Severe Depletion of Mucosal CD4 + T Cells in AIDS-Free Simian Immunodeficiency Virus-Infected Sooty
More informationBEAT-HIV Delaney Collaboratory to Cure HIV-1 Infection by Combination Immunotherapy
BEAT-HIV Delaney Collaboratory to Cure HIV-1 Infection by Combination Immunotherapy We are pleased to announce that the National Institutes of Health (NIH) has awarded nearly $23 million to co-principal
More informationChapter 1. Chapter 1 Concepts. MCMP422 Immunology and Biologics Immunology is important personally and professionally!
MCMP422 Immunology and Biologics Immunology is important personally and professionally! Learn the language - use the glossary and index RNR - Reading, Note taking, Reviewing All materials in Chapters 1-3
More informationElevated expression of LAG-3, but not PD-1, is associated with impaired inkt cytokine production during chronic HIV-1 infection and treatment
Juno et al. Retrovirology (2015) 12:17 DOI 10.1186/s12977-015-0142-z RESEARCH Open Access Elevated expression of LAG-3, but not PD-1, is associated with impaired inkt cytokine production during chronic
More informationNTD Vaccine Design Toolkit and Training Workshop Providence, RI January 05, 2011 Cytokines Leslie P. Cousens, PhD EpiVax, Inc.
NTD Vaccine Design Toolkit and Training Workshop Providence, RI January 05, 2011 Cytokines Leslie P. Cousens, PhD EpiVax, Inc. Cytokines Properties of Cytokines Cytokines are proteins with specific roles
More informationCombination antiretroviral therapy (ART) has. Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure
AIDS RESEARCH AND HUMAN RETROVIRUSES Volume 33, Number S1, 2017 ª Mary Ann Liebert, Inc. DOI: 10.1089/aid.2017.0153 Differentiating Immune Cell Targets in Gut-Associated Lymphoid Tissue for HIV Cure Shahzada
More informationSupplemental Information. The Therapeutic Effect. of Anti-HER2/neu Antibody Depends. on Both Innate and Adaptive Immunity CONTENTS:
Cancer Cell, Volume 18 Supplemental Information The Therapeutic Effect of Anti-HER2/neu Antibody Depends on Both Innate and Adaptive Immunity SaeGwang Park, Zhujun Jiang, Eric D. Mortenson, Liufu Deng,
More informationMeasuring Dendritic Cells
Measuring Dendritic Cells A.D. Donnenberg, V.S. Donnenberg UNIVERSITY of PITTSBURGH CANCER INSTITUTE CCS Longbeach 10_04 Measuring DC Rare event detection The basics of DC measurement Applications Cancer
More informationAnti-α4 Antibody Treatment Blocks Virus Traffic to the Brain and Gut Early, and Stabilizes CNS Injury Late in Infection
Anti-α4 Antibody Treatment Blocks Virus Traffic to the Brain and Gut Early, and Stabilizes CNS Injury Late in Infection The Harvard community has made this article openly available. Please share how this
More informationIL-6Rα IL-6RαT-KO KO. IL-6Rα f/f bp. f/f 628 bp deleted 368 bp. 500 bp
STD H 2 O WT KO IL-6Rα f/f IL-6Rα IL-6RαT-KO KO 1000 bp 500 bp f/f 628 bp deleted 368 bp Supplementary Figure 1 Confirmation of T-cell IL-6Rα deficiency. (a) Representative histograms and (b) quantification
More informationSupplementary Materials for
www.sciencetranslationalmedicine.org/cgi/content/full//49/eaao4/dc Supplementary Materials for TLR agonists induce transient viremia and reduce the viral reservoir in SIV-infected rhesus macaques on antiretroviral
More informationHua Tang, Weiping Cao, Sudhir Pai Kasturi, Rajesh Ravindran, Helder I Nakaya, Kousik
SUPPLEMENTARY FIGURES 1-19 T H 2 response to cysteine-proteases requires dendritic cell-basophil cooperation via ROS mediated signaling Hua Tang, Weiping Cao, Sudhir Pai Kasturi, Rajesh Ravindran, Helder
More informationT Cell Activation. Patricia Fitzgerald-Bocarsly March 18, 2009
T Cell Activation Patricia Fitzgerald-Bocarsly March 18, 2009 Phases of Adaptive Immune Responses Phases of T cell responses IL-2 acts as an autocrine growth factor Fig. 11-11 Clonal Expansion of T cells
More informationMacrophages and Pulmonary Pathogenesis in Young and Aged SIV-Infected Rhesus Macaques
Macrophages and Pulmonary Pathogenesis in Young and Aged SIV-Infected Rhesus Macaques Yanhui CAI (Jenny) Oct. 20 th, 2014 Division of Immunology Tulane National Primate Research Center Covington, LA 1
More informationFrom Bench to Bedside Regulatory T Cells: Can We Make the Police Work for Us?
From Bench to Bedside Regulatory T Cells: Can We Make the Police Work for Us? Sang Mo Kang, MD Qizhi Tang, PhD UCSF Division of Transplantation UCSF Transplant Conference 2012 The Reality of Immunosuppression
More informationImmunology. Anas Abu-Humaidan M.D. Ph.D. Transplant immunology+ Secondary immune deficiency
Immunology Anas Abu-Humaidan M.D. Ph.D. Transplant immunology+ Secondary immune deficiency Transplant Immunology Transplantation is the process of moving cells, tissues or organs from one site to another
More informationImmune response to infection
Immune response to infection Dr. Sandra Nitsche (Sandra.Nitsche@rub.de ) 20.06.2018 1 Course of acute infection Typical acute infection that is cleared by an adaptive immune reaction 1. invasion of pathogen
More informationThe clinical trial information provided in this public disclosure synopsis is supplied for informational purposes only.
The clinical trial information provided in this public disclosure synopsis is supplied for informational purposes only. Please note that the results reported in any single trial may not reflect the overall
More informationAdjuvanting a DNA vaccine with a TLR9 ligand plus Flt3 ligand results in enhanced cellular immunity against the simian immunodeficiency virus
ARTICLE Adjuvanting a DNA vaccine with a TLR9 ligand plus Flt3 ligand results in enhanced cellular immunity against the simian immunodeficiency virus Marcin Kwissa, 1 Rama R. Amara, 1,2 Harriet L. Robinson,
More informationSUPPLEMENTARY INFORMATION
` SUPPLEMENTAL FIGURES doi:10.1038/nature10003 Supplemental Figure 1: RhCMV/SIV vectors establish and indefinitely maintain high frequency SIV-specific T cell responses in diverse tissues: The figure shows
More information