Pro5 MHC Pentamers. Dr. Jeremy Fry. Copyright ProImmune Limited All Rights Reserved

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1 Pro5 MHC Pentamers Dr. Jeremy Fry

2 Pro5 MHC Pentamers The most consistent technology for detecting antigenspecific T cells The most-cited commercially available MHC Multimer Used by most-leading academic and corporate immunology research groups Fastest delivery of custom synthesis Labeling flexibility / Long-term storage Best technical product support and customer service

3 Multiple Labeling Options Pro5 Pentamers Offered as Pre-labeled Complexes R-PE (R-Phycoerythrin)-labeled APC (Allophycocyanin)-labeled Biotin-labeled Pro5 Pentamers Offered as Unlabeled Complexes Pro5 Fluorotags used in conjunction with unlabeled Pentamers Long-term storage of unlabeled Pro5 Pentamers (-80 C) for labeling later Flexibility: no need to decide in advance which label to use for the whole batch

4 Catalog Pentamers More than 200 catalog Pentamer specificities available including: Infectious Disease HIV / SIV CMV, EBV, Influenza Hep B and Hep C Adenovirus LCMV Vaccinia virus Malaria, Chlamydia Tuberculosis, WNV Cancer Antigens Autoimmunity Model Antigens Ovalbumin GFP

5 Delivery time Stock items Catalog Pentamers Shipped in 1-2 days Available items Shipped in 1-2 weeks

6 Custom Pro5 Pentamer Synthesis Human Mouse Simian A*01:01 A*02:01 B*07:02 B*08:01 H-2Db H-2Kb C57BL/6 (H-2b) Mamu-A*01 Mamu-A*02 A*03:01 A*11:01 A*11:03 A*24:02 B*14:02 B*15:01 B*27:05 B*35:01 H-2Kd H-2Dd H-2Ld Balb/c (H-2d) A*29:02 B*35:08 A*68:01 B*40:01 A2Kb B*54:01

7 Custom Pro5 Pentamer Synthesis Process Assess custom MHC/peptide requirements Synthesize peptide / synthesize Pentamer QC and ship

8 Custom Pro5 Pentamer Synthesis Process One-off custom synthesis charge (not applied to repeat orders of the same specificity) All Pentamers Quality Controlled and Guaranteed: biochemical characterization two rounds of protein purification to homogeneity conformational antibody testing flow cytometry analysis to ensure they are within acceptable limits of non-specific staining 6 month / 12 month guarantee of Ag-sp staining for pre-labeled / unlabeled Pentamers Delivery (including peptide synthesis): 4-6 weeks

9 Rapid Custom Pentamer Synthesis For time critical projects Fastest external provider of any custom MHC multimer (commercial or core-facility) Delivery in just 12 working days, including peptide synthesis 50 tests per Pentamer, maximum of 4 x Pentamers per order, subject to availability Rapid synthesis charge of $380 Guaranteed dispatch by 12 th working day following order acceptance or no rapid charge

10 Human or mouse 3 loading options Catalog CD1d tetramers Pre-loaded with alpha-galactosyl ceramide (a-gal-cer) Negative control (mock-loaded) Unloaded (for loading with unique glycolipid) 2 labeling options R-PE APC Delivery time 1-2 days

11 Pricing Pack sizes: 50, 150 or 500 tests 1 test: sufficient to stain 1-2 x 10 6 cells From $1130 for 50 tests; $5470 for 500 tests Cost per test is more than 500 test 10% first order discount is available for 150 tests of Pentamer Bulk discounts available for orders of multiple Pentamer or >500 tests per Pentamer Request a quote: enquiries@proimmune.com

12 Over the past six years I have been using ProImmune s Pro5 Pentamers for the identification and characterization of tumour epitope-specific T cells. Pentamers play an important role in my work to develop new vaccine strategies to induce T cell-mediated immune responses directed against human malignancies. I am always impressed by the knowledge and friendliness of the customer service team, and find the website very user-friendly and well-organized. Dr. John Webb British Columbia Cancer Agency, Canada "Pro5 MHC Pentamers have played an important part in our study on adoptive immunotherapy for cancer and viral infections, post-transplant. We use Pentamers to detect and quantify the populations of antigen-specific T cells in CTL lines made for clinical use. We also use them to detect antigen-specific CTL in the peripheral blood of transplant patients, pre and post-ctl-infusion. The Pentamers were chosen for their stability and for the increased intensity and specificity of the positive population compared to MHC tetramers. We have found Pentamers to be consistent and reliable, and ProImmune's customer service is excellent". Dr. Cath Bollard Baylor College of Medicine, Houston, USA "ProImmune Pro5 MHC Pentamers have been influential in our research to analyze CD8 T cell responses in vivo. Colleagues in the lab recommended the Pentamers to us. For the past two years we have used them for flow cytometry applications to achieve reliable staining across different batches of the same product. I can also recommend ProImmune for their easy ordering process and excellent customer service". Prof. Thomas Brocker Institute for Immunology, University of Munich, Germany