Stage I Stage II Stage III Stage IV
|
|
- Phillip Barrett
- 6 years ago
- Views:
Transcription
1 Harvard-MIT Division of Health Sciences and Technology HST.176: Cellular and Molecular Immunology Course Director: Dr. Shiv Pillai Stage I Stage II Stage III Stage IV Receptor gene Rearrangement Elimination of self-reactive cells Responders Effectors
2 3. BCR MEDIATED MAINTENANCE Y Y FOLLICLE Y SPLEEN 1. pre-bcr MEDIATED POSITIVE SELECTION 2. BCR MEDIATED EMIGRATION BONE MARROW Early pro-b Intermediate pro-b Late pro-b Ψ Large pre-b pre-bcr Small pre-b Y Immature B YY Mature B A B C C' D E F IgM IgD D H to J H rearrangement V H to DJ H V L to J L rearrangement rearrangement
3 Positive selection I Pre-B receptor dependent XLA Syk KO µko Negative selection and Receptor editing Positive selection II Pre-pro B Pro-B I Pro-B2/preB A B C C' Ψ D Y Y E PERIPHERY Y Y F Large pre-b Small Pre-B Immature B Mature B V to DJ out of frame
4 HSC Pre-B receptor expressing cells proliferate and allelically exclude VDJ+ In-frame C' Large pre-b VDJ+ In-frame IL-7 SDF-1 IL-7 SDF-1 IL-7 C' Large pre-b Common lymphoid progenitor A pre-pro-b D-J H B pro-b V-DJ H C pro/pre-b VDJ+ In-frame C' Large pre-b VDJ+ In-frame VDJ- VDJ- C' Large pre-b
5 Commitment Positive selection I Allelic exclusion Positive selection II Negative selection T B
6 Transcriptional regulation of early lymphoid development Tcf-1 -/- Gata-3 -/- DN DP SP Ikaros -/- CLP E2a -/- PU.1 -/- Sox-4 -/- Ebf -/- ProB PreB B Pax-5 -/-
7 Entry versus commitment Commitment implies irreversibility and in wild type B cells has occurred when Ig H-chain gene rearrangement is initiated Certain transcription factors such as EBF and E2A are required to turn on genes required early in B cell development In the absence of Pax-5 cells enter the B lineage but remain highly plastic
8 Pre-B receptor B cell receptor ι/vpreb ω/λ5 Ig β Ig α Ig β Ig α Syk ITAM Syk ITAM Blk/Lyn/Fyn (Src family kinases) Blk/Lyn/Fyn/Fgr (Src family kinases) Btk Other signaling pathways Btk (Bruton's tyrosine kinase) Defective in X-linked agammaglobulinemia) Other signaling pathways
9 ι/vpreb ω/λ5 Ig β Ig α Syk ITAM Blk/Lyn/Fyn Btk 1)Survival 2) Proliferation 3) Allelic exclusion 4) Induction of κ rearrangement 5) Shut off of surrogate light chain expression
10 B cell tolerance See Immunobiology, by Janeway,C., Travers, P., Walport, M. and Capra, J., Garland Publishing, 5th edition, 2001 & Cellular and Molecular Immunology by Abbas, A., Pober, J., and Lichtman, A., W B Saunders; 4th edition.
11 RECEPTOR EDITING Rag gene reexpression Deletion of old V κ-jκ rearrangement New κ or λ lightchain CLONAL DELETION BCR signals induce caspase activation Apoptotic death Multivalent Paucivalent OR ANERGY Chronic crosslinking model Ca++ influx seen but not sustained NFAT and ERK activated normally NFκB and JNK NOT activated
12 Red Pulp White pulp B T B marginal zone and marginal sinus
13 L VDJ µ Poly A sites δ Poly A sites AAAA Cap site ATG Unspliced IgD message AAAA mrna for IgD heavy chain AAAA mrna for IgM heavy chain
14 monosaccharide polysaccharide antigen receptor NO SIGNAL SIGNAL TRANSDUCTION
15 Microbial antigens Bacterial LPS B-1 Y? Microbial antigens HSC pro-b/ pre-b IgM YY B IgD Y B-1 Fetal Liver HSC No TdT Limited diversity B cells with bias towards multivalent TI-1 antigens Y B-1 B-1 cells are self-renewing and express CD5 The B-1/CD5 B "lineage"
16 THREE DISTINCT TYPES OF PERIPHERAL B LYMPHOCYTES CD21 HIGH Y IgM CD1 SPLEEN PERITONEUM / MUCOSAL SITES MARGINAL ZONE B CELLS B-1 B CELLs IgM Y CD5 FOLLICULAR B CELLS IgM IgD YY CD21
17 MZ and Follicular B cells See Immunobiology, by Janeway,C., Travers, P., Walport, M. and Capra, J., Garland Publishing, 5th edition, 2001 & Cellular and Molecular Immunology by Abbas, A., Pober, J., and Lichtman, A., W B Saunders; 4th edition.
18 Are only chosen B cells selected by endogenous antigens? OR Do all B cells get tickled via the antigen receptor?
19 Mutation Signal Strength MZ FO B-1 BCR BCR "No BCR signals" Other pathways Btk Btk "Weak BCR signals" Other pathways PLCγ2 PLCγ2 "Weak BCR signals" +* - - Other pathways PKCβ PKCβ " Weak and Intermediate BCR signals" None "Weak, Intermediate, and Strong BCR signals" + + +
20 LONG-LIVED PERIPHERAL B CELL POPULATIONS Strong BCR Signals.B-1 cells Intermediate strength BCR signals.follicular B cells Relatively weak BCR signals..mz B cells
21 CD21 CD1 Y MZ B CELLS IgM Y Y IgD Y IgD FI Newly formed B cells SPLEEN Y FIII NF FII FOLLICLE pre-bcr MEDIATED POSITIVE SELECTION BCR MEDIATED EMIGRATION BONE MARROW Early pro-b Intermediate pro-b Late pro-b Ψ Large pre-b pre-bcr Small pre-b Y Immature B Y Mature B A B C C' D E F IgM? IgD D H to J H rearrangement V H to DJ H rearrangement V L to J L rearrangement
22 activated T TCR CD4 gp 39/CD40L Y BCR MHC class II (loaded) CD40 Y B
23 T cell areas follicle (B cell zone) Cortex Medulla afferent lymphatics artery vein efferent lymphatic 1.Dendritic cells (interdigitating) in T cell zones 2. Follicular dendritic cells in B cell areas 3. Macrophages everywhere
24 Red pulp 2 Focal aggregate X Y I Ag T Y Y 3 Germinal center B 4 White pulp
25 T B somatic mutation isotype switching T high affinity T cell Memory B cells low affinity APC X?Receptor diversification and rescue Follicular Dendritic cell Plasma cells
26 Activation of APCs via CD40 to release IL-12 and drive a TH1 type response CD40 T activated gp 39/CD40L?Signals to T cell TCR CD4 gp 39/CD40L Y BCR Y MHC class II (loaded) B CD40 Required for T-dependent immune responses -proliferation -class switching -germinal center formation -somatic mutation CD40L mutations lead to X-linked hyper-igm syndrome
27 PALS (T cell area) Follicle ( B cell area) Dark zone Light zone } Germinal center memory B cells plasma cells Centroblasts Centrocytes apoptosis V gene hypermutation
28 JH Cµ Cδ Cγ3 Cγ1 Cα1 Cγ2 Cγ4 Cε Cα2
29 VDJ Sµ Cµ Cδ Sγ3 Cγ3 Sγ1 Cγ1 Sα Cα γ2b, γ2a and ε Sγ3 Cγ3 Cδ Sγ1 VDJ Cµ Cγ1 Looping out and deletion Sµ Sα Cα VDJ Sα Cα Switched to IgA
30 Switch regions and I-region promoters VDJ C ienh Iµ Sµ µ δ γ3 α Iα Sα Iγ3 Sγ3 Cα3'E 3'αE HS3B HS4 γ2b, γ2a and ε C regions LCR Iµ Sµ µ unspliced pre-mrna Iµ µ spliced Iµ transcript
31 Class Switching (Murine) 1. IL-4 promotes switching to IgG1 and IgE 2. TGF- β promotes switching to IgA 3. γ-ifn promotes switching to IgG2a
32 Somatic mutation-i 1. Point substitutions. Non-templated single base changes in rearranged H- and L-chain V region genes 2. Requires T cell help, occurs in centrocytes to 10-3 base pairs/generation 4. Bell shaped curve of mutations starts in leader intron and ends about 1.5 kb downstream 5. Hotspot motifs 6. Transitions more common than transversions
33 SOMATIC MUTATION -II 7. Requires enhancer 8. Mechanism:AID DEPENDENT DNA DEAMINATION a. Cytosines converted to uracils b. Replication or error prone repair generates mutations 9. Accessibility?? Need for transcription??
34 AID required for both class switching and somatic mutation AID is a novel Activation induced cytidine deaminase Related to a protein involved in RNA editing Required for class switching and also for somatic mutation Aid-/- mice have large germinal centers Humans lacking AID present with hyper IgM syndrome
35 3. BCR MEDIATED MAINTENANCE Y Y FOLLICLE Y SPLEEN 1. pre-bcr MEDIATED POSITIVE SELECTION 2. BCR MEDIATED EMIGRATION BONE MARROW Early pro-b Intermediate pro-b Late pro-b Ψ Large pre-b pre-bcr Small pre-b Y Immature B YY Mature B A B C C' D E F IgM IgD D H to J H rearrangement V H to DJ H rearrangement V L to J L rearrangement
36 For more information and examples, see Immunobiology, by Janeway,C., Travers, P., Walport, M. and Capra, J., Garland Publishing, 5th edition, 2001 & Cellular and Molecular Immunology by Abbas, A., Pober, J., and Lichtman, A., W B Saunders; 4th edition.
B Lymphocyte Development and Activation
Harvard-MIT Division of Health Sciences and Technology HST.176: Cellular and Molecular Immunology Course Director: Dr. Shiv Pillai 09/26/05; 9 AM Shiv Pillai B Lymphocyte Development and Activation Recommended
More informationmembrane form secreted form 13 aa 26 aa K K V V K K 3aa
Harvard-MIT Division of Health Sciences and Technology HST.176: Cellular and Molecular Immunology Course Director: Dr. Shiv Pillai secreted form membrane form 13 aa 26 aa K K V V K K 3aa Hapten monosaccharide
More informationFollicular Lymphoma. ced3 APOPTOSIS. *In the nematode Caenorhabditis elegans 131 of the organism's 1031 cells die during development.
Harvard-MIT Division of Health Sciences and Technology HST.176: Cellular and Molecular Immunology Course Director: Dr. Shiv Pillai Follicular Lymphoma 1. Characterized by t(14:18) translocation 2. Ig heavy
More informationChapter 11. B cell generation, Activation, and Differentiation. Pro-B cells. - B cells mature in the bone marrow.
Chapter B cell generation, Activation, and Differentiation - B cells mature in the bone marrow. - B cells proceed through a number of distinct maturational stages: ) Pro-B cell ) Pre-B cell ) Immature
More informationChapter 11. B cell generation, Activation, and Differentiation. Pro-B cells. - B cells mature in the bone marrow.
Chapter B cell generation, Activation, and Differentiation - B cells mature in the bone marrow. - B cells proceed through a number of distinct maturational stages: ) Pro-B cell ) Pre-B cell ) Immature
More informationIntroduction. Introduction. Lymphocyte development (maturation)
Introduction Abbas Chapter 8: Lymphocyte Development and the Rearrangement and Expression of Antigen Receptor Genes Christina Ciaccio, MD Children s Mercy Hospital January 5, 2009 Lymphocyte development
More informationDevelopment of B and T lymphocytes
Development of B and T lymphocytes What will we discuss today? B-cell development T-cell development B- cell development overview Stem cell In periphery Pro-B cell Pre-B cell Immature B cell Mature B cell
More informationAntigen-Independent B-Cell Development Bone Marrow
Antigen-Independent B-Cell Development Bone Marrow 1. DNA rearrangements establish the primary repertoire, creating diversity 2. Allelic exclusion ensures that each clone expresses a single antibody on
More informationκ λ Antigen-Independent B-Cell Development Bone Marrow Ordered Rearrangement of Ig Genes During B-Cell Development in the Bone Marrow
Antigen-Independent B-Cell Development Bone Marrow 1. DNA rearrangements establish the primary repertoire, creating diversity 2. Allelic exclusion ensures that each clone expresses a single antibody on
More informationThe Development of Lymphocytes: B Cell Development in the Bone Marrow & Peripheral Lymphoid Tissue Deborah A. Lebman, Ph.D.
The Development of Lymphocytes: B Cell Development in the Bone Marrow & Peripheral Lymphoid Tissue Deborah A. Lebman, Ph.D. OBJECTIVES 1. To understand how ordered Ig gene rearrangements lead to the development
More informationIg light chain rearrangement: Rescue pathway
B Cell Development Ig light chain rearrangement: Rescue pathway There is only a 1:3 chance of the join between the V and J region being in frame Vk Jk Ck Non-productive Rearrangement Light chain has a
More informationB cell activation and antibody production. Abul K. Abbas UCSF
1 B cell activation and antibody production Abul K. Abbas UCSF 2 Lecture outline B cell activation; the role of helper T cells in antibody production Therapeutic targeting of B cells 3 Principles of humoral
More informationSPECIFIC IMMUNITY = ACQUIRED IMMUNITY = ADAPTIVE IMMUNITY SPECIFIC IMMUNITY - BASIC CHARACTERISTIC
SPECIFIC IMMUNITY - BASIC CHARACTERISTIC SPECIFIC IMMUNITY = ACQUIRED IMMUNITY = ADAPTIVE IMMUNITY BASIC TERMINOLOGY SPECIFIC IMMUNITY humoral mediated with antibodies cellular mediated with T lymphocytes
More informationIntroduction. Abbas Chapter 10: B Cell Activation and Antibody Production. General Features. General Features. General Features
Introduction Abbas Chapter 10: B Cell Activation and Antibody Production January 25, 2010 Children s Mercy Hospitals and Clinics Humoral immunity is mediated by secreted antibodies (Ab) Ab function to
More informationAndrea s SI Session PCB Practice Test Test 3
Practice Test Test 3 READ BEFORE STARTING PRACTICE TEST: Remember to please use this practice test as a tool to measure your knowledge, and DO NOT use it as your only tool to study for the test, since
More informationT cell Receptor. Chapter 9. Comparison of TCR αβ T cells
Chapter 9 The αβ TCR is similar in size and structure to an antibody Fab fragment T cell Receptor Kuby Figure 9-3 The αβ T cell receptor - Two chains - α and β - Two domains per chain - constant (C) domain
More informationImmune Regulation and Tolerance
Immune Regulation and Tolerance Immunoregulation: A balance between activation and suppression of effector cells to achieve an efficient immune response without damaging the host. Activation (immunity)
More informationGeneration of antibody diversity October 18, Ram Savan
Generation of antibody diversity October 18, 2016 Ram Savan savanram@uw.edu 441 Lecture #10 Slide 1 of 30 Three lectures on antigen receptors Part 1 : Structural features of the BCR and TCR Janeway Chapter
More informationAllergy and Immunology Review Corner: Chapter 1 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti.
Allergy and Immunology Review Corner: Chapter 1 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti. Chapter 1: Overview of Immunology Prepared by David Scott, MD, Scripps
More informationT cell maturation. T-cell Maturation. What allows T cell maturation?
T-cell Maturation What allows T cell maturation? Direct contact with thymic epithelial cells Influence of thymic hormones Growth factors (cytokines, CSF) T cell maturation T cell progenitor DN DP SP 2ry
More informationAntibodies and T Cell Receptor Genetics Generation of Antigen Receptor Diversity
Antibodies and T Cell Receptor Genetics 2008 Peter Burrows 4-6529 peterb@uab.edu Generation of Antigen Receptor Diversity Survival requires B and T cell receptor diversity to respond to the diversity of
More informationB cell development in the bone marrow.
development in the bone. s develop from multipotent haematopoietic stem s in the bone that produce lymphoid progenitors which in turn generate s. s with a unique antibody specificity develop by initial
More informationAdaptive Immunity: Humoral Immune Responses
MICR2209 Adaptive Immunity: Humoral Immune Responses Dr Allison Imrie 1 Synopsis: In this lecture we will review the different mechanisms which constitute the humoral immune response, and examine the antibody
More informationThe Adaptive Immune Response. B-cells
The Adaptive Immune Response B-cells The innate immune system provides immediate protection. The adaptive response takes time to develop and is antigen specific. Activation of B and T lymphocytes Naive
More informationMolecular Pathology of Lymphoma (Part 1) Rex K.H. Au-Yeung Department of Pathology, HKU
Molecular Pathology of Lymphoma (Part 1) Rex K.H. Au-Yeung Department of Pathology, HKU Lecture outline Time 10:00 11:00 11:15 12:10 12:20 13:15 Content Introduction to lymphoma Review of lymphocyte biology
More informationImmunobiology 7. The Humoral Immune Response
Janeway Murphy Travers Walport Immunobiology 7 Chapter 9 The Humoral Immune Response Copyright Garland Science 2008 Tim Worbs Institute of Immunology Hannover Medical School 1 The course of a typical antibody
More informationT Cell Activation. Patricia Fitzgerald-Bocarsly March 18, 2009
T Cell Activation Patricia Fitzgerald-Bocarsly March 18, 2009 Phases of Adaptive Immune Responses Phases of T cell responses IL-2 acts as an autocrine growth factor Fig. 11-11 Clonal Expansion of T cells
More informationImmunodermatology. Danielle Tartar, MD, PhD Assistant Clinical Professor Co-Director of Inpatient Dermatology University of California - Davis
Immunodermatology Danielle Tartar, MD, PhD Assistant Clinical Professor Co-Director of Inpatient Dermatology University of California - Davis Outline Adaptive Immunity: T and B cell development, activation,
More informationInnate immunity (rapid response) Dendritic cell. Macrophage. Natural killer cell. Complement protein. Neutrophil
1 The immune system The immune response The immune system comprises two arms functioning cooperatively to provide a comprehensive protective response: the innate and the adaptive immune system. The innate
More informationT cell development October 28, Dan Stetson
T cell development October 28, 2016 Dan Stetson stetson@uw.edu 441 Lecture #13 Slide 1 of 29 Three lectures on T cells (Chapters 8, 9) Part 1 (Today): T cell development in the thymus Chapter 8, pages
More informationOverview B cell development T cell development
Topics Overview B cell development T cell development Lymphocyte development overview (Cont) Receptor diversity is produced by gene rearrangement and is random Includes specificities that will bind to
More informationChapter 5. Generation of lymphocyte antigen receptors
Chapter 5 Generation of lymphocyte antigen receptors Structural variation in Ig constant regions Isotype: different class of Ig Heavy-chain C regions are encoded in separate genes Initially, only two of
More informationThe Humoral Immune Response
The Humoral Immune Response BBS755 March 19, 2015 Robert Woodland AS8-1057 Robert.Woodland@umassmed.edu Reading: Janeway Chapters 10 and 12 Starting point A naive B cell is recirculating resting lymphocyte
More informationAndrea s Final Exam Review PCB 3233 Spring Practice Final Exam
NOTE: Practice Final Exam Although I am posting the answer key for this practice exam, I want you to use this practice to gauge your knowledge, and try to figure out the right answer by yourself before
More informationDefensive mechanisms include :
Acquired Immunity Defensive mechanisms include : 1) Innate immunity (Natural or Non specific) 2) Acquired immunity (Adaptive or Specific) Cell-mediated immunity Humoral immunity Two mechanisms 1) Humoral
More informationExamples of questions for Cellular Immunology/Cellular Biology and Immunology
Examples of questions for Cellular Immunology/Cellular Biology and Immunology Each student gets a set of 6 questions, so that each set contains different types of questions and that the set of questions
More informationChapter 19: IgE-Dependent Immune Responses and Allergic Disease
Allergy and Immunology Review Corner: Chapter 19 of Cellular and Molecular Immunology (Seventh Edition), by Abul K. Abbas, Andrew H. Lichtman and Shiv Pillai. Chapter 19: IgE-Dependent Immune Responses
More informationMicrobiology 204. Background Slides on T Cell Development For Preparation for Flipped Classroom setting. Art Weiss.
Microbiology 204 Background Slides on T Cell Development For Preparation for Flipped Classroom setting Art Weiss October 23, 2015 Thymic Lobule Structure From: Immunobiology, Janeway, et al., 5th edition
More informationT Cell Receptor & T Cell Development
T Cell Receptor & T Cell Development Questions for the next 2 lectures: How do you generate a diverse T cell population with functional TCR rearrangements? How do you generate a T cell population that
More informationPLAN. Réponses B thymodépendantes et thymoindépendantes. B cell development and classification. B cell activation. Thymodependent B(2) cell response
Réponses B thymodépendantes et thymoindépendantes PLAN B cell development and classification B cell activation Thymodependent B(2) cell response BMC 423 (IF) - 2007 Antonino Nicoletti Thymo-independent
More informationRecommended reading: Abbas et al. 5th edition, chapters 7 and 10; Janeway and Travers, 5th edition, chapter 7.
Harvard-MIT Division of Health Sciences and Technology HST.176: Cellular and Molecular Immunology Course Director: Dr. Shiv Pillai 10/05/05; 11 AM Shiv Pillai T Lymphocyte Development Recommended reading:
More informationAntigen Presentation and T Lymphocyte Activation. Shiv Pillai MD, PhD Massachusetts General Hospital Harvard Medical School. FOCiS
1 Antigen Presentation and T Lymphocyte Activation Shiv Pillai MD, PhD Massachusetts General Hospital Harvard Medical School FOCiS 2 Lecture outline Overview of T cell activation and the rules of adaptive
More informationThe Adaptive Immune Response. T-cells
The Adaptive Immune Response T-cells T Lymphocytes T lymphocytes develop from precursors in the thymus. Mature T cells are found in the blood, where they constitute 60% to 70% of lymphocytes, and in T-cell
More informationPrinciples of Adaptive Immunity
Principles of Adaptive Immunity Chapter 3 Parham Hans de Haard 17 th of May 2010 Agenda Recognition molecules of adaptive immune system Features adaptive immune system Immunoglobulins and T-cell receptors
More informationT Cell Effector Mechanisms I: B cell Help & DTH
T Cell Effector Mechanisms I: B cell Help & DTH Ned Braunstein, MD The Major T Cell Subsets p56 lck + T cells γ δ ε ζ ζ p56 lck CD8+ T cells γ δ ε ζ ζ Cα Cβ Vα Vβ CD3 CD8 Cα Cβ Vα Vβ CD3 MHC II peptide
More informationall of the above the ability to impart long term memory adaptive immunity all of the above bone marrow none of the above
1. (3 points) Immediately after a pathogen enters the body, it faces the cells and soluble proteins of the innate immune system. Which of the following are characteristics of innate immunity? a. inflammation
More informationSINGLE CHOICE. 5. The gamma invariant chain binds to this molecule during its intracytoplasmic transport. A TCR B BCR C MHC II D MHC I E FcγR
A Name: Group: SINGLE CHOICE 1. Which is the most important ligand of TLR5? A endospore B flagellin C polysaccharide capsule D DNA E pilus 2. The antibody-binding site is formed primarily by... A the constant
More informationThe development of T cells in the thymus
T cells rearrange their receptors in the thymus whereas B cells do so in the bone marrow. The development of T cells in the thymus The lobular/cellular organization of the thymus Immature cells are called
More informationB Cell Activation and the Humoral Immune Response. Micro 204. Molecular and Cellular Immunology 2012 Lecturer: Jason Cyster
B Cell Activation and the Humoral Immune Response Micro 204. Molecular and Cellular Immunology 2012 Lecturer: Jason Cyster Constitutive BCR signaling is necessary for B cell survival V-region IgM constant-region
More information5/1/13. The proportion of thymus that produces T cells decreases with age. The cellular organization of the thymus
T cell precursors migrate from the bone marrow via the blood to the thymus to mature 1 2 The cellular organization of the thymus The proportion of thymus that produces T cells decreases with age 3 4 1
More informationCHAPTER 9 BIOLOGY OF THE T LYMPHOCYTE
CHAPTER 9 BIOLOGY OF THE T LYMPHOCYTE Coico, R., Sunshine, G., (2009) Immunology : a short course, 6 th Ed., Wiley-Blackwell 1 CHAPTER 9 : Biology of The T Lymphocytes 1. 2. 3. 4. 5. 6. 7. Introduction
More informationB Cell Activation and the Humoral Immune Response. Micro 204. Molecular and Cellular Immunology 2013 Lecturer: Jason Cyster
B Cell Activation and the Humoral Immune Response Micro 204. Molecular and Cellular Immunology 2013 Lecturer: Jason Cyster Vaccine Quest Vaccines are needed that induce broadly neutralizing antibodies
More informationPutting it Together. Stephen Canfield Secondary Lymphoid System. Tonsil Anterior Cervical LN s
Putting it Together Stephen Canfield smc12@columbia.edu Secondary Lymphoid System Tonsil Anterior Cervical LN s Axillary LN s Mediastinal/Retroperitoneal LN s Thoracic Duct Appendix Spleen Inguinal LN
More informationPATHOGEN INNOCUOUS ANTIGEN. No Danger- very low expression of costimulatory ligands Signal One Only
Harvard-MIT Division of Health Sciences and Technology HST.176: Cellular and Molecular Immunology Course Director: Dr. Shiv illai AICD Naive Activated Effector Memory Activated Effector Naive AICD Activated
More informationChapter 10 (pages ): Differentiation and Functions of CD4+ Effector T Cells Prepared by Kristen Dazy, MD, Scripps Clinic Medical Group
FIT Board Review Corner September 2015 Welcome to the FIT Board Review Corner, prepared by Andrew Nickels, MD, and Sarah Spriet, DO, senior and junior representatives of ACAAI's Fellows-In-Training (FITs)
More informationImmunology Lesson plan for international medical students
Immunology Lesson plan for international medical students Kermanshah University of medical sciences (KUMS) international affairs School of medicine Department of Immunology Lecturers: Dr. Ali Gorgin Karaji
More informationHOST DEFENSE SMALL GROUP PROBLEM SOLVING SESSION CLINICAL IMMUNOLOGIC ASSAYS-II
Host Defense 2011 Small Group Problem Solving Session Clinical Immunologic Assays II HOST DEFENSE SMALL GROUP PROBLEM SOLVING SESSION CLINICAL IMMUNOLOGIC ASSAYS-II Small Group Classrooms LEARNING GOAL
More informationAntigen Presentation and T Lymphocyte Activation. Abul K. Abbas UCSF. FOCiS
1 Antigen Presentation and T Lymphocyte Activation Abul K. Abbas UCSF FOCiS 2 Lecture outline Dendritic cells and antigen presentation The role of the MHC T cell activation Costimulation, the B7:CD28 family
More informationEffector mechanisms of cell-mediated immunity: Properties of effector, memory and regulatory T cells
ICI Basic Immunology course Effector mechanisms of cell-mediated immunity: Properties of effector, memory and regulatory T cells Abul K. Abbas, MD UCSF Stages in the development of T cell responses: induction
More informationTwo categories of immune response. immune response. infection. (adaptive) Later immune response. immune response
Ivana FELLNEROVÁ E-mail: fellneri@hotmail.com, mob. 732154801 Basic immunogenetic terminology innate and adaptive immunity specificity and polymorphism immunoglobuline gene superfamily immunogenetics MHC
More informationT cell and Cell-mediated immunity
T cell and Cell-mediated immunity Lu Linrong ( 鲁林荣 ) PhD Laboratory of Immune Regulation Institute of Immunology Zhejiang University, it School of Medicine i Medical Research Building B815-819 Email: Lu.Linrong@gmail.com
More informationAdaptive immunity. Adaptive Immunity. Principles of immune defense. Adaptive immunity. against extracellular or intracellular pathogens
Principles of immune defense Toxicology Course Vienna MODULE 12 Immunotoxicology, Allergy July 2, 2008 Prof. Erika Jensen-Jarolim, MD Dept. of Pathophysiology Medical University Vienna Gastrointestinaltrakt:
More informationImmunology. T-Lymphocytes. 16. Oktober 2014, Ruhr-Universität Bochum Karin Peters,
Immunology T-Lymphocytes 16. Oktober 2014, Ruhr-Universität Bochum Karin Peters, karin.peters@rub.de The role of T-effector cells in the immune response against microbes cellular immunity humoral immunity
More informationremember that T-cell signal determine what antibody to be produce class switching somatical hypermutation all takes place after interaction with
بسم هللا الرحمن الرحيم The last lecture we discussed the antigen processing and presentation and antigen recognition then the activation by T lymphocyte and today we will continue with B cell recognition
More informationThe T cell receptor for MHC-associated peptide antigens
1 The T cell receptor for MHC-associated peptide antigens T lymphocytes have a dual specificity: they recognize polymporphic residues of self MHC molecules, and they also recognize residues of peptide
More informationBasic Immunology. Immunological tolerance. Cellular and molecular mechanisms of the immunological tolerance. Lecture 23 rd
Basic Immunology Lecture 23 rd Immunological tolerance Cellular and molecular mechanisms of the immunological tolerance Tolerated skin grafts on MHC (H2) identical mice TOLERANCE & AUTOIMMUNITY Upon encountering
More informationImmunology 2017: Lecture 12 handout. Secondary lymphoid organs. Dr H Awad
Immunology 2017: Lecture 12 handout Secondary lymphoid organs Dr H Awad INTRODUCTION So far we discussed the cells of the immune system and how they recognize their antigens and get stimulated. The number
More informationAttribution: University of Michigan Medical School, Department of Microbiology and Immunology
Attribution: University of Michigan Medical School, Department of Microbiology and Immunology License: Unless otherwise noted, this material is made available under the terms of the Creative Commons Attribution
More informationChapter 1. Chapter 1 Concepts. MCMP422 Immunology and Biologics Immunology is important personally and professionally!
MCMP422 Immunology and Biologics Immunology is important personally and professionally! Learn the language - use the glossary and index RNR - Reading, Note taking, Reviewing All materials in Chapters 1-3
More informationChapter 2 (pages 22 33): Cells and Tissues of the Immune System. Prepared by Kristen Dazy, MD, Scripps Clinic Medical Group
Allergy and Immunology Review Corner: Cellular and Molecular Immunology, 8th Edition By Abul K. Abbas, MBBS; Andrew H. H. Lichtman, MD, PhD; and Shiv Pillai, MBBS, PhD. Chapter 2 (pages 22 33): Cells and
More informationPRECURSOR LYMHPOID NEOPLASMS. B lymphoblastic leukaemia/lymphoma T lymphoblastic leukaemia/lymphoma
PRECURSOR LYMHPOID NEOPLASMS B lymphoblastic leukaemia/lymphoma T lymphoblastic leukaemia/lymphoma B lymphoblastic leukaemia/lymphoma Definition: B lymphoblastic leukaemia/lymphoma is a neoplasm of precursor
More informationHelminth worm, Schistosomiasis Trypanosomes, sleeping sickness Pneumocystis carinii. Ringworm fungus HIV Influenza
Helminth worm, Schistosomiasis Trypanosomes, sleeping sickness Pneumocystis carinii Ringworm fungus HIV Influenza Candida Staph aureus Mycobacterium tuberculosis Listeria Salmonella Streptococcus Levels
More informationEssentials in Immunology Prof. Anjali A. Karande Department of Biochemistry Indian Institute of Science, Bangalore
Essentials in Immunology Prof. Anjali A. Karande Department of Biochemistry Indian Institute of Science, Bangalore Lecture No. # 07 Development and differentiation of B cells Today s lecture is on the
More informationEffector T Cells and
1 Effector T Cells and Cytokines Andrew Lichtman, MD PhD Brigham and Women's Hospital Harvard Medical School 2 Lecture outline Cytokines Subsets of CD4+ T cells: definitions, functions, development New
More informationIntroduction to Immunology Part 2 September 30, Dan Stetson
Introduction to Immunology Part 2 September 30, 2016 Dan Stetson stetson@uw.edu 441 Lecture #2 Slide 1 of 26 CLASS ANNOUNCEMENT PLEASE NO TREE NUTS IN CLASS!!! (Peanuts, walnuts, almonds, cashews, etc)
More informationLESSON 2: THE ADAPTIVE IMMUNITY
Introduction to immunology. LESSON 2: THE ADAPTIVE IMMUNITY Today we will get to know: The adaptive immunity T- and B-cells Antigens and their recognition How T-cells work 1 The adaptive immunity Unlike
More informationAdaptive Immune System
Short Course on Immunology Adaptive Immune System Bhargavi Duvvuri Ph.D IIIrd Year (Immunology) bhargavi@yorku.ca Supervisor Dr.Gillian E Wu Professor, School of Kinesiology and Health Sciences York University,
More informationAdaptive immune responses: T cell-mediated immunity
MICR2209 Adaptive immune responses: T cell-mediated immunity Dr Allison Imrie allison.imrie@uwa.edu.au 1 Synopsis: In this lecture we will discuss the T-cell mediated immune response, how it is activated,
More informationAcquired Immunity 2. - Vaccines & Immunological Memory - Wataru Ise. WPI Immunology Frontier Research Center (IFReC) Osaka University.
Acquired Immunity 2 - Vaccines & Immunological Memory - Wataru Ise WPI Immunology Frontier Research Center (IFReC) Osaka University Outline 1. What is vaccine (vaccination)? 2. What is immunological memory?
More informationT cell and Cell-mediated immunity
T cell and Cell-mediated immunity ( 第十章 第十二章第十二章 ) Lu Linrong ( 鲁林荣 ) PhD Laboratory of Immune Regulation Institute of Immunology Zhejiang University, School of Medicine Medical Research Building B815-819
More informationStructure and Function of Antigen Recognition Molecules
MICR2209 Structure and Function of Antigen Recognition Molecules Dr Allison Imrie allison.imrie@uwa.edu.au 1 Synopsis: In this lecture we will examine the major receptors used by cells of the innate and
More informationSupporting Information Table of Contents
Supporting Information Table of Contents Supporting Information Figure 1 Page 2 Supporting Information Figure 2 Page 4 Supporting Information Figure 3 Page 5 Supporting Information Figure 4 Page 6 Supporting
More informationCD21 and CD24 Co-expression: A Translational Model between Mouse and Human
Loma Linda University TheScholarsRepository@LLU: Digital Archive of Research, Scholarship & Creative Works Loma Linda University Electronic Theses, Dissertations & Projects 6-2014 CD21 and CD24 Co-expression:
More informationLectins: selected topics 3/2/17
Lectins: selected topics 3/2/17 Selected topics Regulation of T-cell receptor signaling Thymic selection of self vs. non-self T-cells Essentials of Glycobiology Second Edition Signaling pathways associated
More informationTolerance, autoimmunity and the pathogenesis of immunemediated inflammatory diseases. Abul K. Abbas UCSF
Tolerance, autoimmunity and the pathogenesis of immunemediated inflammatory diseases Abul K. Abbas UCSF Balancing lymphocyte activation and control Activation Effector T cells Tolerance Regulatory T cells
More informationHematopoiesis. Hematopoiesis. Hematopoiesis
Chapter. Cells and Organs of the Immune System Hematopoiesis Hematopoiesis- formation and development of WBC and RBC bone marrow. Hematopoietic stem cell- give rise to any blood cells (constant number,
More informationMCAT Biology - Problem Drill 16: The Lymphatic and Immune Systems
MCAT Biology - Problem Drill 16: The Lymphatic and Immune Systems Question No. 1 of 10 1. Which of the following statements about pathogens is true? Question #01 (A) Both viruses and bacteria need to infect
More informationMechanisms of anti-sm B cell activation in autoimmune Fas-deficient mice
Mechanisms of anti-sm B cell activation in autoimmune Fas-deficient mice Kara Lynne Conway A dissertation submitted to the faculty of the University of North Carolina at Chapel Hill in partial fulfillment
More informationImmunodeficiency. (1 of 2)
Immunodeficiency (1 of 2) Primary immunodeficiency diseases Innate or adaptive Most are detected in infancy (6 months 2 years) Rare but some mild genetic forms exist in many individuals Defects in Innate
More informationThe Adaptive Immune Responses
The Adaptive Immune Responses The two arms of the immune responses are; 1) the cell mediated, and 2) the humoral responses. In this chapter we will discuss the two responses in detail and we will start
More informationT-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:
Interactions between innate immunity & adaptive immunity What happens to T cells after they leave the thymus? Naïve T cells exit the thymus and enter the bloodstream. If they remain in the bloodstream,
More informationT-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:
Interactions between innate immunity & adaptive immunity What happens to T cells after they leave the thymus? Naïve T cells exit the thymus and enter the bloodstream. If they remain in the bloodstream,
More informationSOPHOMORE DENTAL/OPTOMETRY MICROBIOLOGY SECTION: IMMUNOLOGY CELLS AND ORGANS OF THE IMMUNE SYSTEM
SOPHOMORE DENTAL/OPTOMETRY MICROBIOLOGY SECTION: IMMUNOLOGY CELLS AND ORGANS OF THE IMMUNE SYSTEM Lecturer: Dr. John F Kearney 934-6557 jfk@uab.edu Objectives: To learn: 1) the basic cell types that make
More informationPractice Test. Test 5
Practice Test Test 5 1) A plasma cell can directly differentiate from: a. An activated B cell. b. Isotype switched, somatically hypermutated centrocyte c. Memory B cell. d. A & B e. All of the above. 2)
More informationMajor Histocompatibility Complex (MHC) and T Cell Receptors
Major Histocompatibility Complex (MHC) and T Cell Receptors Historical Background Genes in the MHC were first identified as being important genes in rejection of transplanted tissues Genes within the MHC
More informationPrimary Immunodeficiency
Primary Immunodeficiency DiGeorge Syndrome Severe Combined Immunodeficiency SCID X-Linked Agammaglobulinemia Common variable immunodeficiency (CVID) IgA deficiency Hyper- IgM Syndrome Wiskott-Aldrich syndrome
More informationACTIVATION AND EFFECTOR FUNCTIONS OF CELL-MEDIATED IMMUNITY AND NK CELLS. Choompone Sakonwasun, MD (Hons), FRCPT
ACTIVATION AND EFFECTOR FUNCTIONS OF CELL-MEDIATED IMMUNITY AND NK CELLS Choompone Sakonwasun, MD (Hons), FRCPT Types of Adaptive Immunity Types of T Cell-mediated Immune Reactions CTLs = cytotoxic T lymphocytes
More informationOverview of immunology
Introduction Overview of immunology Masayuki Miyasaka, MD, PhD Interdisciplinary Program for Biomedical Sciences WPI Immunology Frontier Research Center (IFReC) Osaka University Inside our body, we have
More informationAdaptive Immunity: Specific Defenses of the Host
17 Adaptive Immunity: Specific Defenses of the Host SLOs Differentiate between innate and adaptive immunity, and humoral and cellular immunity. Define antigen, epitope, and hapten. Explain the function
More information