Serological screening for syphilis in HIV-infected individuals: is a non-treponemal test adequate in the era of increasing of new syphilis infections?

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "Serological screening for syphilis in HIV-infected individuals: is a non-treponemal test adequate in the era of increasing of new syphilis infections?"

Transcription

1 Abstract no. WEPE 494 Serological screening for syphilis in HIV-infected individuals: is a non-treponemal test adequate in the era of increasing of new syphilis infections? G.Chrysos 1, D.Karageorgopoulos 1,3, A.Lazaridou 2, C.Poulopoulou 2, K.Chrysou 1, P.Chatziastros 1, E.Friligou 1, P. Mistylis 1, A.Sotiropoulos 1, K.Digalaki 2, D.Paraskeva 1,3 1. Infectious Diseases Unit; 2. Department of Microbiology, Tzaneio General Hospital, Piraeus, 3. Hellenic Center for Disease Control and Prevention, Athens, Greece

2 Background Traditionally, serologic screening for syphilis involves performing a nontreponemal test (e.g. VDRL, RPR) first, which is confirmed, if positive, with a treponemal test. The diagnostic accuracy of non-treponemal tests for syphilis might be lower in patients with HIV-infection. False positive tests due to immune activation can be common. False negative tests can also be observed, particularly in seriously immunocompromised patients. The reversal of the traditional order of serologic testing for syphilis (i.e., performing a treponemal test first) is gaining interest with regard to HIV-infected patients. The advent of automated enzyme immunoassay tests facilitates the change in the screening algorithm. We aimed to assess serologic screening for syphilis with the use of the Fluorescent Treponemal Antibody Absorption test (FTA-Abs) in HIV-positive patients.

3 Patients and methods Because of a steep increase in the incidence of syphilis in high-risk patient groups in Greece, FTA-Abs testing has been included, along with Rapid Plasma Reagin (RPR) testing, in the routine (every 6-12 months) serologic screening protocol for syphilis, followed at the HIV Clinic (Infections Diseases Unit) of Tzaneio General Hospital, Piraeus, Greece, in January We retrospectively examined the records of the Microbiology Laboratory of our hospital to identify the patients followed in the HIV Clinic who had undergone serologic testing for syphilis between 1/1/2010 and 1/10/2011. We reviewed all of the available paper and electronic medical records of these patients and extracted relevant demographic, clinical and laboratory data.

4 Results (1) During the study period, 171 unique HIV-infected, sexually-active patients were screened at least once with FTA-Abs for syphilis, out of the 350 patients followed in our Clinic. 135/171 (78,9%) had a negative FTA-Abs test result. None of the above 135 patients had a positive non-treponemal RPR test.

5 Results (2) 23/171 (13,5%) had a positive FTA-Abs test result. Out of the 23 patients with positive FTA-Abs, 9 (39,1%) also had a positive RPR test. Five out of the 9 patients with both tests positive, were found to have early syphilis. The remaining 14/23 (60,9%) FTA-Abs positive patients had negative RPR. 3/14 patients underwent additional serologic testing at a reference laboratory and did not have a positive result for another treponemal test (Treponema pallidum particle agglutination assay (TP-PA) or Treponema pallidum enzyme immunoassay (TP-EIA)). Thus, the positive FTA-Abs test result was considered as false-positive. 4/14 patients had a positive clinical history for syphilis or a history of previously positive serologic tests for syphilis.

6 Results (3) 13/171 (7,6%) additional patients had an indeterminate or weekly positive FTA-Abs test result Out of the 13 patients with indeterminate/weekly positive FTA-Abs, 1 (7,7%) patient had positive RPR and early syphilis, 6 (50,0%) had negative RPR, For the remaining 6 patients, data for a recent RPR test were not available.

7 Results (4) FTA + FTA - FTA ± TOTAL RPR (5.8%) RPR (94.2) TOTAL 23 (13.5%) 135 (78.9%) 13 (7.6%) 171 (100%)

8 Main findings and discussion The use of FTA-Abs in the serologic screening for syphilis in HIV-infected patients resulted in the identification of all of the 10 patients who had a positive RPR. 20/171 (11,7%) patients had a positive or indeterminate FTA-Abs test with a negative RPR. These patients could have had false positive FTA-Abs, treated syphilis, or late latent syphilis (false negative RPR). Although the sensitivity of RPR in diagnosing syphilis is considered very high, case reports have described RPR-seronegative syphilis in HIV-positive patients. A negative non-treponemal test may not rule out syphilis in patients with advanced immunosuppression. Reinfection may be difficult to rule out in some patients, and reactivation or relapse of a previously treated infection is also possible for an HIV-positive person. The diagnostic value of FTA-ABS can be greater in HIV-infected patients with latent syphilis. FTA-ABS turns positive earlier and remains positive longer than RPR.

9 Conclusion The use of FTA-Abs in the serologic screening for syphilis in HIV-infected patients can lead to the identification of a greater number of patients with probable syphilis than with RPR alone. Clinicians should consider the sensitivity of FTA-ABS to be high in HIV-infected persons with untreated syphilis beyond the primary stage of infection. The patients with a positive FTA-Abs test and a negative RPR test need confirmatory testing with another treponemal test. If asymptomatic patients have a positive non-treponemal test and negative confirmatory treponemal test results, it is very unlikely that they have active syphilis.

10 Literature cited 1. Centers for Disease Control and Prevention (CDC). Syphilis testing algorithms using treponemal tests for initial screening-- four laboratories, New York City, MMWR Morb. Mortal. Wkly. Rep. 2008; 57: Workowski KA, Berman S. Sexually transmitted diseases treatment guidelines, MMWR Recomm Rep 2010; 59:1-110.

Use of Treponemal Immunoassays for Screening and Diagnosis of Syphilis

Use of Treponemal Immunoassays for Screening and Diagnosis of Syphilis Use of Treponemal Immunoassays for Screening and Diagnosis of Syphilis Guidance for Medical Providers and Laboratories in California These guidelines were developed by the California Department of Public

More information

Direct Comparison of the Traditional and Reverse Syphilis Screening Algorithms

Direct Comparison of the Traditional and Reverse Syphilis Screening Algorithms JCM Accepts, published online ahead of print on 16 November 2011 J. Clin. Microbiol. doi:10.1128/jcm.05636-11 Copyright 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All

More information

SYPHILIS (Treponema pallidum) IMMEDIATE NOTIFICATION STD PROGRAM

SYPHILIS (Treponema pallidum) IMMEDIATE NOTIFICATION STD PROGRAM SYPHILIS (Treponema pallidum) IMMEDIATE NOTIFICATION STD PROGRAM Event Name: Event Time Period: Clinical Description (CDC 2014) Syphilis 180 days Syphilis is a complex sexually transmitted disease that

More information

2/13/ Graphic photographs or cartoons used during this presentation might be offensive to some; for this I apologize in advance.

2/13/ Graphic photographs or cartoons used during this presentation might be offensive to some; for this I apologize in advance. Leon Bullard, MD, MA Medical Consultant, DHEC, DADE The 23 rd Annual APRN Conference Charleston, SC February 24, 2017 1. Provide a brief (very) review of the syphilis story. 2. Define and discuss the stages

More information

Identifying false-positive syphilis antibody results using a semi-quantitative

Identifying false-positive syphilis antibody results using a semi-quantitative CVI Accepts, published online ahead of print on 20 April 2011 Clin. Vaccine Immunol. doi:10.1128/cvi.05066-11 Copyright 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All

More information

Syphilis Seroconversion and Reinfection among Individuals Entering Baltimore City Booking and Intake Center Rosemary Nabaweesi Amy Winkler

Syphilis Seroconversion and Reinfection among Individuals Entering Baltimore City Booking and Intake Center Rosemary Nabaweesi Amy Winkler Syphilis Seroconversion and Reinfection among Individuals Entering Baltimore City Booking and Intake Center 1998-2004 Rosemary Nabaweesi Amy Winkler 1 Treponema pallidum Organism that causes syphilis Gram

More information

Syphilis among MSM: Clinical Care and Public Health Reporting

Syphilis among MSM: Clinical Care and Public Health Reporting Massachusetts Department of Public Health Bureau of Infectious Disease and Laboratory Sciences Syphilis among MSM: Clinical Care and Public Health Reporting Kevin Ard, MD, MPH, Medical Director, National

More information

Evaluation of Reagin Screen, a New Serological Test for Syphilis

Evaluation of Reagin Screen, a New Serological Test for Syphilis JOURNAL OF CUNICAL MICROBIOLoGY, Aug. 1976, p. 145-150 Copyright 1976 American Society for Microbiology Vol. 4, No. 2 Printed in U.S.A. Evaluation of Reagin Screen, a New Serological Test for Syphilis

More information

February 3, Division of Dockets Management (HFA 305) Food and Drug Administration 5630 Fishers Lane, Rm Rockville, MD 20852

February 3, Division of Dockets Management (HFA 305) Food and Drug Administration 5630 Fishers Lane, Rm Rockville, MD 20852 February 3, 2014 Division of Dockets Management (HFA 305) Food and Drug Administration 5630 Fishers Lane, Rm. 1061 Rockville, MD 20852 ATTN: Leslie Kux, Assistant Commissioner for Policy Ref: [Docket No.

More information

PROPOSED USE This method is used for determining plasma reagins in human sera.

PROPOSED USE This method is used for determining plasma reagins in human sera. Agglutination Assay -glass slide For "in vitro" use only Store at 2-8 C INTRODUCRION Syphilis is a sexually transmitted disease caused by Treponema pallidum. The main tests used to diagnose syphilis are:

More information

Evaluation of a New Rapid Plasma Reagin Card Test as a Screening Test for Syphilis

Evaluation of a New Rapid Plasma Reagin Card Test as a Screening Test for Syphilis JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 1982, p. 286-290 0095-1 137/82/080286-05$02.00/0 Vol. 16, No. 2 Evaluation of a New Rapid Plasma Reagin Card Test as a Screening Test for Syphilis MARY W. PERRYMAN,'*

More information

VDRL v/s TPHA for diagnosis of syphilis among HIV sero-reactive patients in a tertiary care hospital

VDRL v/s TPHA for diagnosis of syphilis among HIV sero-reactive patients in a tertiary care hospital ISSN: 2319-7706 Volume 3 Number 5 (2014) pp. 726-730 http://www.ijcmas.com Original Research Article VDRL v/s TPHA for diagnosis of syphilis among HIV sero-reactive patients in a tertiary care hospital

More information

Clinical Application of New Treponemal Antibody Test in Blood Donors

Clinical Application of New Treponemal Antibody Test in Blood Donors Clinical Application of New Treponemal Antibody Test in Blood Donors Parichart Permpikul, MD Department of Transfusion Medicine Faculty of Medicine Siriraj Hospital Mahidol University Bangkok, Thailand

More information

Sexually Transmitted Diseases Treatment Guidelines, 2015

Sexually Transmitted Diseases Treatment Guidelines, 2015 Morbidity and Mortality Weekly Report Recommendations and Reports / Vol. 64 / No. 3 June 5, 2015 Sexually Transmitted Diseases Treatment Guidelines, 2015 U.S. Department of Health and Human Services Centers

More information

Learning Objectives. Syphilis. Lessons. Epidemiology: Disease in the U.S. Syphilis Definition. Transmission. Treponema pallidum

Learning Objectives. Syphilis. Lessons. Epidemiology: Disease in the U.S. Syphilis Definition. Transmission. Treponema pallidum Learning Objectives Syphilis Treponema pallidum 1 Upon completion of this content, the learner will be able to 1. Describe the epidemiology of syphilis in the U.S. 2. Describe the pathogenesis of T. pallidum.

More information

Didactic Series. STD Screening & Management: Syphilis. Christian B. Ramers, MD, MPH

Didactic Series. STD Screening & Management: Syphilis. Christian B. Ramers, MD, MPH Didactic Series STD Screening & Management: Syphilis Christian B. Ramers, MD, MPH Assistant Medical Director Family Health Centers of San Diego Ciaccio Memorial Clinic 3/26/15 ACCREDITATION STATEMENT:

More information

Evaluation of Treponemal Serum Tests Performed on Cerebrospinal Fluid for Diagnosis of Neurosyphilis

Evaluation of Treponemal Serum Tests Performed on Cerebrospinal Fluid for Diagnosis of Neurosyphilis Evaluation of Treponemal Serum Tests Performed on Cerebrospinal Fluid for Diagnosis of Neurosyphilis Jeannette Guarner, Emory University Heather Jost, Emory University Allan Pillay, Emory University Yongcheng

More information

Syphilis. sera from the Venereal Disease Serology Laboratory. Serum Bank, Centers for Disease Control, Atlanta,

Syphilis. sera from the Venereal Disease Serology Laboratory. Serum Bank, Centers for Disease Control, Atlanta, JOURNAL OF CLINICAL MICROBIOLOGY, Oct. 1981, p. 441-445 0095-1137/81/0441-05$02.00/0 Vol. 14, No. 4 Specificity, Sensitivity, and Reproducibility Among the Fluorescent Treponemal Antibody-Absorption Test,

More information

Screening for Syphilis With the Treponemal Immunoassay: Analysis of Discordant Serology Results and Implications for Clinical Management

Screening for Syphilis With the Treponemal Immunoassay: Analysis of Discordant Serology Results and Implications for Clinical Management MAJOR ARTICLE Screening for Syphilis With the Treponemal Immunoassay: Analysis of Discordant Serology Results and Implications for Clinical Management Ina U. Park, 1,2 Joan M. Chow, 1 Gail Bolan, 1 Mark

More information

Wei-Qiang He, Huan-Li Wang, Dao-Qing Zhong, Lu-Yang Lin, Xiao-Shan Qiu, Ri-Dong Yang

Wei-Qiang He, Huan-Li Wang, Dao-Qing Zhong, Lu-Yang Lin, Xiao-Shan Qiu, Ri-Dong Yang Int J Clin Exp Pathol 2015;8(5):5775-5780 www.ijcep.com /ISSN:1936-2625/IJCEP0006331 Original Article Treponemal antibody in CSF and cellular immunity in peripheral blood of syphilitic patients with persisting

More information

CHAPTER-X SYPHILIS R.KAVITHA, M.PHARM, LECTURER, DEPARTMENT OF PHARMACEUTICS, SRM COLLEGE OF PHARMACY, SRM UNIVERSITY, KATTANKULATHUR.

CHAPTER-X SYPHILIS R.KAVITHA, M.PHARM, LECTURER, DEPARTMENT OF PHARMACEUTICS, SRM COLLEGE OF PHARMACY, SRM UNIVERSITY, KATTANKULATHUR. CHAPTER-X SYPHILIS R.KAVITHA, M.PHARM, LECTURER, DEPARTMENT OF PHARMACEUTICS, SRM COLLEGE OF PHARMACY, SRM UNIVERSITY, KATTANKULATHUR. Trepanoma pallidum D. Clinical Infection: Syphilis Transmission Usually

More information

EVALUATION OF ENZYME IMMUNOASSAY AND IMMUNOBLOT TESTING FOR THE DIAGNOSIS OF SYPHILIS IN ALBERTA

EVALUATION OF ENZYME IMMUNOASSAY AND IMMUNOBLOT TESTING FOR THE DIAGNOSIS OF SYPHILIS IN ALBERTA EVALUATION OF ENZYME IMMUNOASSAY AND IMMUNOBLOT TESTING FOR THE DIAGNOSIS OF SYPHILIS IN ALBERTA Arto Ohinmaa, Anderson Chuck, Thanh Nguyen, Janice Varney, Philip Jacobs Supported by a financial contribution

More information

Evaluation of a Treponema pallidum Enzyme Immunoassay as a Screening Test for Syphilis

Evaluation of a Treponema pallidum Enzyme Immunoassay as a Screening Test for Syphilis CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, JUly 1994, p. 477-481 Vol. 1, No. 4 1071-412X/94/$04.00+0 Copyright C 1994, American Society for Microbiology Evaluation of a Treponema pallidum Enzyme Immunoassay

More information

Sexually Transmitted Infections: Your Doorway to Opportunities!

Sexually Transmitted Infections: Your Doorway to Opportunities! Sexually Transmitted Infections: Your Doorway to Opportunities! Laura Hinkle Bachmann, MD, MPH Professor of Medicine Wake Forest University Health Sciences Winston-Salem, NC Co-Director Alabama/North Carolina

More information

Performance of SD Bioline Syphilis 3.0 for the Diagnosis of Syphilis a UPFR in Immunology of CHU-JRA

Performance of SD Bioline Syphilis 3.0 for the Diagnosis of Syphilis a UPFR in Immunology of CHU-JRA International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 8 (2017) pp. 783-788 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.608.099

More information

What s New: STDs. Seema Nayak, MD Johns Hopkins University

What s New: STDs. Seema Nayak, MD Johns Hopkins University What s New: STDs Seema Nayak, MD Johns Hopkins University Financial Disclosures None Will discuss experimental gonorrhea treatment options that are in the published literature Objectives Discuss screening

More information

26. Screening for Syphilis

26. Screening for Syphilis 26. Screening for Syphilis RECOMMENDATION Routine serologic screening for syphilis is recommended for all pregnant women and for persons at increased risk of infection (see Clinical Inter - vention). See

More information

Sex, Sores, Science, and Surveillance: Syphilis in the 21 st Century (U046)

Sex, Sores, Science, and Surveillance: Syphilis in the 21 st Century (U046) Sex, Sores, Science, and Surveillance: Syphilis in the 21 st Century (U046) Kenneth A. Katz, MD, MSc, MSCE Dermatologist, Kaiser Permanente, San Francisco, CA AAD Annual Meeting, San Diego, CA February

More information

SYPHILIS HEALTH CHECK

SYPHILIS HEALTH CHECK SYPHILIS HEALTH CHECK Rev. P, 02/17 CLIA Complexity: WAIVED for Fingerstick Whole Blood Specimens ONLY For in vitro diagnostic use only Rx Only A Certificate of Waiver is required to perform this test

More information

SYPHILIS (REPORTABLE)

SYPHILIS (REPORTABLE) SYPHILIS (REPORTABLE) PREAMBLE In BC, the diagnosis of syphilis is determined by the BCCDC Provincial STI/HIV Clinic physician directly or in coordination with the*physician or nurse practitioner (NP)

More information

Evaluation Report MHRA 04007

Evaluation Report MHRA 04007 April 2004 NUMBER Evaluation Report MHRA 04007 Five Syphilis Agglutination Assays MHRA Evaluation Report MHRA Report number 04007 Crown Copyright 60 (Free to NHS) WHAT YOU CAN EXPECT FROM MHRA EVALUATION

More information

Division of Dermatology Dr A Motau

Division of Dermatology Dr A Motau Division of Dermatology Dr A Motau CASE 1 Histopathology H&E H&E H&E Wartin Starry Immunohistochemical stain for T. pallidum Investigations FBC, U&E, LFT Normal T. pallidum Abs Reactive RPR screen

More information

Sex, Sores, Science, and Surveillance: Syphilis in the 21 st Century (U046)

Sex, Sores, Science, and Surveillance: Syphilis in the 21 st Century (U046) Sex, Sores, Science, and Surveillance: Syphilis in the 21 st Century (U046) Kenneth A. Katz, MD, MSc, MSCE Dermatologist, Kaiser Permanente, San Francisco, CA AAD Annual Meeting, Orlando, FL March 4, 2017

More information

Syphilis, caused by the spirochetal bacterium Treponema pallidum

Syphilis, caused by the spirochetal bacterium Treponema pallidum MINIREVIEW Recent Trends in the Serologic Diagnosis of Syphilis Muhammad G. Morshed, a Ameeta E. Singh b Department of Pathology & Laboratory Medicine, Faculty of Medicine, University of British Columbia,

More information

Elimination of Congenital Syphilis in South Africa Where are we and what needs to be done?

Elimination of Congenital Syphilis in South Africa Where are we and what needs to be done? Elimination of Congenital Syphilis in South Africa Where are we and what needs to be done? Presented by: Dr Saiqa Mullick (Director: Implementation Science, Wits RHI) Co-authors: Diantha Pillay (Researcher:

More information

Clinical Practice Guideline with Delegation of Function

Clinical Practice Guideline with Delegation of Function 1 of 8 1.0 Purpose 1.1 To improve and focus efforts to treat those at risk for undiagnosed/untreated incubating, primary, secondary, or early latent infectious syphilis. 2.0 Scope and Goal 2.1 Antibiotic

More information

The etiological agent of syphilis is Treponema pallidum.

The etiological agent of syphilis is Treponema pallidum. CANADIAN STI BEST PRACTICE LABORATORY GUIDELINES The laboratory diagnosis of syphilis Sam Ratnam PhD MPH FCCM S Ratnam. The laboratory diagnosis of syphilis. Can J Infect Dis Med Microbiol 2005;16(1):45-51.

More information

Learning Objectives. Epidemiology 5/3/2013. Treponema pallidum Diagnosis, Treatment and Prevention. Anne Rompalo, MD, ScM Professor of Medicine

Learning Objectives. Epidemiology 5/3/2013. Treponema pallidum Diagnosis, Treatment and Prevention. Anne Rompalo, MD, ScM Professor of Medicine Treponema pallidum Diagnosis, Treatment and Prevention Anne Rompalo, MD, ScM Professor of Medicine Learning Objectives Describe the epidemiology of syphilis in the U.S.Describe the pathogenesis of Treponema

More information

Treponema pallidum Total ELISA Kit

Treponema pallidum Total ELISA Kit Treponema pallidum Total ELISA Kit Catalog Number KA0970 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

TEN YEARS OF SYPHILIS TRENDS IN THE NORTHERN CAPE PROVINCE, SOUTH AFRICA, UTILISING THE NHLS CORPORATE DATA WAREHOUSE

TEN YEARS OF SYPHILIS TRENDS IN THE NORTHERN CAPE PROVINCE, SOUTH AFRICA, UTILISING THE NHLS CORPORATE DATA WAREHOUSE TEN YEARS OF SYPHILIS TRENDS IN THE NORTHERN CAPE PROVINCE, SOUTH AFRICA, UTILISING THE NHLS CORPORATE DATA WAREHOUSE Introduction Ngormbu Ballah 1,2,3, Lazarus Kuonza 1,3, Gloria De Gita 2, Alfred Musekiwa

More information

Trends in Sexually Transmitted Infections (STIs) C. Junda Woo, MD, MPH, Medical Director San Antonio Metropolitan Health District June 3, 2017

Trends in Sexually Transmitted Infections (STIs) C. Junda Woo, MD, MPH, Medical Director San Antonio Metropolitan Health District June 3, 2017 Trends in Sexually Transmitted Infections (STIs) C. Junda Woo, MD, MPH, Medical Director San Antonio Metropolitan Health District June 3, 2017 1 Speaker Disclosure Dr. Woo has disclosed that she has no

More information

Primary Sample Manual Infectious Serology Issue No Effective Date: 20/09/17 Page 1 of 15 EUROFINS BIOMNIS

Primary Sample Manual Infectious Serology Issue No Effective Date: 20/09/17 Page 1 of 15 EUROFINS BIOMNIS Issue No. 2.02 Effective Date: 20/09/17 Page 1 of 15 Written / Revised By: Dr. Mike Louw, Medical Director Date: Reviewed By: Date: Dr. Sinead Kelly, Infectious Serology Consultant Authorised By: Jean-Sébastien

More information

NOTE: Please append modifier 33 when indicated. If modifier 33 is not appended, regular plan benefits will be applied.

NOTE: Please append modifier 33 when indicated. If modifier 33 is not appended, regular plan benefits will be applied. Policy name: Preventive Health Guidelines - Men The following chart contains procedure and diagnosis code combinations that identify services covered under HMSA's Preventive Health s policy. * For professional

More information

Use of enzyme immunoassay as treponemal screening test in syphilis diagnosis

Use of enzyme immunoassay as treponemal screening test in syphilis diagnosis Hong Kong J. Dermatol. Venereol. (2006) 14, 189-195 Review Article Use of enzyme immunoassay as treponemal screening test in syphilis diagnosis WL Leung and KM Kam Serological methods play a major role

More information

Answering your daily challenges in ASO detection. Anti-Streptolysin O. rapid test. rheumajet ASO I NFECTIOU S DI SEASES.

Answering your daily challenges in ASO detection. Anti-Streptolysin O. rapid test. rheumajet ASO I NFECTIOU S DI SEASES. Answering your daily challenges in ASO detection Anti-Streptolysin O rheumajet ASO I NFECTIOU S DI SEASES I N F E C T I O U S D I S E A S E S Anti-streptolysin O Anti-Streptolysin O (ASO) antibodies appear

More information

Serologic Response to Treatment in Syphilis

Serologic Response to Treatment in Syphilis Serologic Response to Treatment in Syphilis 7 Neuza Satomi Sato Center of Immunology, Institute Adolfo Lutz São Paulo, SP Brazil 1. Introduction Serologic monitoring is the way to determine adequate treatment

More information

Perspective Sexually Transmitted Infections and HIV: Diagnosis and Treatment

Perspective Sexually Transmitted Infections and HIV: Diagnosis and Treatment Sexually Transmitted Infections and HIV Volume 20 Issue 1 April/May 2012 Perspective Sexually Transmitted Infections and HIV: Diagnosis and Treatment Accurate assessment, diagnosis, and treatment of sexually

More information

Comparison of Semi-Quantitative RPR Test and TPHA for Serodiagnosis of Syphilis

Comparison of Semi-Quantitative RPR Test and TPHA for Serodiagnosis of Syphilis ORIGINAL ARTICLE pissn 0976 3325 eissn 2229 6816 Open Access Article www.njcmindia.org Comparison of Semi-Quantitative RPR Test and TPHA for Serodiagnosis of Syphilis Nasiruddin M Shaikh 1, Tanmay K Mehta

More information

Treponema Pallidum Total Antibody ELISA

Treponema Pallidum Total Antibody ELISA For Research Use Only. Not for use in Diagnostic Procedures. INTENDED USE The GenWay, Inc. Treponima pallidum Total ELISA Kit is intended for the detection of IgG, IgM and IgA antibody to Treponima pallidum

More information

January Dear Physician:

January Dear Physician: Richard F. Daines, M.D. Commissioner Wendy E. Saunders Executive Deputy Commissioner January 2009 Dear Physician: The purpose of this letter is to bring your attention to the significant increase in reported

More information

The Alphabet Soup of Viral Hepatitis Testing

The Alphabet Soup of Viral Hepatitis Testing The Alphabet Soup of Viral Hepatitis Testing August 18, 2011 Patricia Slev, PhD, DABCC Medical Director, Serologic Hepatitis and Retrovirus Laboratory, ARUP Laboratories Assistant Professor of Pathology,

More information

Congenital Syphilis. Chokechai Rongkavilit, M.D. Medical Director Pediatric Infectious Diseases

Congenital Syphilis. Chokechai Rongkavilit, M.D. Medical Director Pediatric Infectious Diseases Congenital Syphilis Chokechai Rongkavilit, M.D. Medical Director Pediatric Infectious Diseases Case 3-hour-old infant male 39 weeks gestation born vaginally without complications. Mother 9 weeks pregnancy:

More information

Appendix B: Provincial Case Definitions for Reportable Diseases

Appendix B: Provincial Case Definitions for Reportable Diseases Ministry of Health and Long-Term Care Infectious Diseases Protocol Appendix B: Provincial Case Definitions for Reportable Diseases Disease: Hepatitis A Revised March 2017 Hepatitis A 1.0 Provincial Reporting

More information

Treponema pallidum IgM ELISA Kit

Treponema pallidum IgM ELISA Kit Treponema pallidum IgM ELISA Kit Catalog Number KA0969 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle

More information

Passive Particle Agglutination Test for Detection of antibodies to Treponema Pallidum

Passive Particle Agglutination Test for Detection of antibodies to Treponema Pallidum REF : 201626, 201633, 226414, 201640 EDMA CODE : 15 01 03 03 00 (For In Vitro Diagnostic Use) Passive Particle Agglutination Test for Detection of antibodies to Treponema Pallidum TABLE OF CONTENTS 1.

More information

Syphilis Outbreak Investigation Report

Syphilis Outbreak Investigation Report ` Office of the Chief Medical Officer of Health Syphilis Outbreak Investigation Report Office of the Chief Medical Officer of Health Health il 2014 Table of Contents Summary... 1 Acronyms... 1 Introduction...

More information

Revisions to the Syphilis Surveillance Case Definitions, 2018

Revisions to the Syphilis Surveillance Case Definitions, 2018 National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Prevention Revisions to the Syphilis Surveillance Case Definitions, 2018 Sarah Kidd, MD, MPH Medical Epidemiologist Division of STD Prevention

More information

Khalil G. Ghanem, MD, PhD Associate Professor of Medicine Johns Hopkins University School of Medicine. April 2, 2014

Khalil G. Ghanem, MD, PhD Associate Professor of Medicine Johns Hopkins University School of Medicine. April 2, 2014 Khalil G. Ghanem, MD, PhD Associate Professor of Medicine Johns Hopkins University School of Medicine April 2, 2014 E-mail your questions for the presenter to: maphtc@jhsph.edu DISCLOSURES OFF- LABEL USES

More information

Management of infants at risk of congenital syphilis

Management of infants at risk of congenital syphilis Management of infants at risk of congenital syphilis Version: Issued: Review date: Author: Dr Sanjay Patel (Paediatric Infectious Diseases Consultant), Dr Emanuela Pelosi (Consultant Virologist), Dr Mildred

More information

Syphilis sero-positivity in recently admitted and long-term psychiatric inpatients: Screening, prevalence and diagnostic profile

Syphilis sero-positivity in recently admitted and long-term psychiatric inpatients: Screening, prevalence and diagnostic profile Syphilis sero-positivity in recently admitted and long-term psychiatric inpatients: Screening, prevalence and diagnostic profile M P Henning, C Krüger, L Fletcher Department of Psychiatry, University of

More information

Analysis of Western Blotting (Immunoblotting) Technique in

Analysis of Western Blotting (Immunoblotting) Technique in JOURNAL OF CLINICAL MICROBIOLOcY, Mar. 1994. p. 629-633 0095-1 137/94/$04.00+() Copyright 1994. American Society for Microbiology Vol. 32. No. 3 Analysis of Western Blotting (Immunoblotting) Technique

More information

Clinical Practice Objectives

Clinical Practice Objectives STD Essentials for the Busy Clinician Susan S. Philip, MD, MPH Assistant Professor, Division of Infectious Diseases, UCSF Director, STD Prevention and Control Services San Francisco Department of Public

More information

STORAGE: Store at 2 to 30 C (36 to 86 F)

STORAGE: Store at 2 to 30 C (36 to 86 F) 61-1004-0 20 Test Kit DPP HIV-SYPHILIS Assay CAUTION- Investigational Device. Limited by Federal (or United States) Law to Investigational Use. FOR IN VITRO DIAGNOSTIC USE FOR PROFESSIONAL USE ONLY A Qualitative

More information

Diagnosis of congenital syphilis and toxoplasmosis

Diagnosis of congenital syphilis and toxoplasmosis 14. White, A.B. et al. (2014) Antiretroviral interventions for preventing breast milk transmission of HIV. Cochrane Database Syst. Rev. CD011323. doi:10.1002/ 14651858.CD011323 15. Jamieson, D.J. et al.

More information

Treponema Pallidum (TP) Antibody Test

Treponema Pallidum (TP) Antibody Test Treponema Pallidum (TP) Antibody Test Instructions For Use Format: Cassette For: Catalog Number: VEL-001-TP Specimen: Serum/Plasma * Please read the instructions carefully before use INTENDED USE Velotest

More information

Managing Syphilis in the HIV-infected Patient

Managing Syphilis in the HIV-infected Patient Managing Syphilis in the HIV-infected Patient Christopher S. Hall, MD, MS*, Jeffrey D. Klausner, MD, MPH, and Gail A. Bolan, MD Address *University of California San Francisco, Sexually Transmitted Disease

More information

Design Verification. Form:

Design Verification. Form: Design Verification SYPHILIS TPHA liquid CONTENTS 1 Function... 2 2 Sensitivity... 2 Preparation of Serum Control Panel... 2 Kit Controls Positive and Negative... 3 3 Traceability... 3 4 Specificity and

More information

Evaluation of recomwell Treponema, a novel recombinant antigen-based enzyme-linked immunosorbent assay for the diagnosis of syphilis

Evaluation of recomwell Treponema, a novel recombinant antigen-based enzyme-linked immunosorbent assay for the diagnosis of syphilis ORIGINAL ARTICLE Evaluation of recomwell Treponema, a novel recombinant antigen-based enzyme-linked immunosorbent assay for the diagnosis of syphilis V. Sambri 1, A. Marangoni 1, M. A. Simone 1, A. D Antuono

More information

Evaluation and Determination of the Sensitivity and Specificity of a Treponema Pallidum Dried Blood Spot Method for Serologic Diagnosis of Syphilis

Evaluation and Determination of the Sensitivity and Specificity of a Treponema Pallidum Dried Blood Spot Method for Serologic Diagnosis of Syphilis Georgia State University ScholarWorks @ Georgia State University Public Health Theses School of Public Health Fall 12-20-2012 Evaluation and Determination of the Sensitivity and Specificity of a Treponema

More information

Biomarkers for Infectious Disease Diagnostics in the Developing World:

Biomarkers for Infectious Disease Diagnostics in the Developing World: Biomarkers for Infectious Disease Diagnostics in the Developing World: Diagnosis of Syphilis in Asymptomatic Pregnant Women John Todd, Paul Neuwald, Laura Penny and Mickey Urdea August 2006 Halteres Associates,

More information

SIGNIFICANCE AND BACKGROUND

SIGNIFICANCE AND BACKGROUND FTA-ABS Test System FA71 INTENDED USE The ZEUS IFA Fluorescent Treponemal Antibody-Absorption (FTA-ABS) Test System is designed for the qualitative determination of antibodies to Treponema pallidum, and

More information

Skills workshop: Screening tests for syphilis

Skills workshop: Screening tests for syphilis 1D Skills workshop: Screening tests for syphilis Objectives When you have completed this skills workshop you should be able to: Screen a patient for syphilis with the syphilis rapid test and the RPR card

More information

Medicine. Observational Study. 1. Introduction OPEN

Medicine. Observational Study. 1. Introduction OPEN Observational Study Medicine Could lengthening minocycline therapy better treat early syphilis? Li-Li Shao, MM, Rui Guo, MM, Wei-Jie Shi, MM, Yuan-Jun Liu, MD, Bin Feng, MB, Long Han, MB, Quan-Zhong Liu,

More information

For Qualitative Detection of IgG Antibodies to Treponema pallidum

For Qualitative Detection of IgG Antibodies to Treponema pallidum 01-580 96 Test Set INTENDED USE ReQuest SYPHILIS IgG is an immunoenzymatic method for the qualitative detection of IgG antibodies to Treponema pallidum in human serum by a manual technique. The test may

More information

Technical Bulletin No. 121

Technical Bulletin No. 121 CPAL Central Pennsylvania Alliance Laboratory Technical Bulletin No. 121 January 31, 2014 Lyme Blot, IgG and IgM - Now Performed at CPAL Contact: J. Matthew Groeller, 717.851.1416 Operations Manager, Clinical

More information

Institution: Procedure NO.: Page 1 of 7

Institution: Procedure NO.: Page 1 of 7 Institution: Procedure NO.: Page 1 of 7 Subject/Title: ASI RPR CARD TEST FOR SYPHILIS Doc#: 6004-900 CLSI Effective Date: 12/12 Supersedes Revision/Date: 06/12 Revision: 12/12 Supersedes Procedure # Prepared

More information

ORIGINAL ARTICLE. Trends in Seroprevalence of Hepatitis B, Hepatitis C, HIV, and Syphilis Infections in Iranian Blood Donors from 2003 to 2005

ORIGINAL ARTICLE. Trends in Seroprevalence of Hepatitis B, Hepatitis C, HIV, and Syphilis Infections in Iranian Blood Donors from 2003 to 2005 ORIGINAL ARTICLE Hepatitis Monthly 2009; 9(1): 24-28 Trends in Seroprevalence of Hepatitis B, Hepatitis C, HIV, and Syphilis Infections in Iranian Blood Donors from 2003 to 2005 Hossein Khedmat 1*, Seyed-Moayed

More information

Lymphogranuloma Venereum (LGV) Surveillance Project

Lymphogranuloma Venereum (LGV) Surveillance Project Lymphogranuloma Venereum (LGV) Surveillance Project Lymphogranuloma venereum (LGV) is a systemic, sexually transmitted disease (STD) caused by a type of Chlamydia trachomatis (serovars L1, L2, L3) that

More information

For in vitro diagnostic use Tests Tests Tests Tests Tests CPT Code: 86592

For in vitro diagnostic use Tests Tests Tests Tests Tests CPT Code: 86592 For in vitro diagnostic use Catalog Number Kit Size 900025 25 Tests 900100 100 Tests 900500 500 Tests 9005000 5000 Tests 90010000 10000 Tests CPT Code: 86592 1 INTENDED USE: The ASI RPR (rapid plasma reagin)

More information

Update on Sexually Transmitted Infections Jeanne Marrazzo, MD, MPH

Update on Sexually Transmitted Infections Jeanne Marrazzo, MD, MPH Update on Sexually Transmitted Infections Jeanne Marrazzo, MD, MPH Division of Infectious Diseases University of Alabama at Birmingham School of Medicine Birmingham, Alabama Outline Syphilis in all its

More information

Single-Dose Azithromycin versus Penicillin G Benzathine for the Treatment of Early Syphilis

Single-Dose Azithromycin versus Penicillin G Benzathine for the Treatment of Early Syphilis The new england journal of medicine original article Single-Dose Azithromycin versus Penicillin G Benzathine for the Treatment of Early Syphilis Gabriele Riedner, M.D., Ph.D., Mary Rusizoka, Dipl.Med.,

More information

WHO Information Note on the Use of Dual HIV/Syphilis Rapid Diagnostic Tests (RDT)

WHO Information Note on the Use of Dual HIV/Syphilis Rapid Diagnostic Tests (RDT) WHO Information Note on the Use of Dual HIV/Syphilis Rapid Diagnostic Tests (RDT) Information Note Advice for countries using or planning to introduce dual HIV/syphilis RDT in antenatal services and other

More information

Sensitivity and specificity of a rapid point-of-care test for active yaws: a comparative study

Sensitivity and specificity of a rapid point-of-care test for active yaws: a comparative study Sensitivity and specificity of a rapid point-of-care test for active yaws: a comparative study Telek Ayove, Wendy Houniei, Regina Wangnapi, Sibauk V Bieb, Walter Kazadi, Lisol-Nirau Luke, Clement Manineng,

More information

SP.718 Special Topics at Edgerton Center: D-Lab Health: Medical Technologies for the Developing World

SP.718 Special Topics at Edgerton Center: D-Lab Health: Medical Technologies for the Developing World MIT OpenCourseWare http://ocw.mit.edu SP.718 Special Topics at Edgerton Center: D-Lab Health: Medical Technologies for the Developing World Spring 2009 For information about citing these materials or our

More information

CATIE Webinar Series (Part 1) Gay Men s Health & HIV Prevention in Canada

CATIE Webinar Series (Part 1) Gay Men s Health & HIV Prevention in Canada CATIE Webinar Series (Part 1) Gay Men s Health & HIV Prevention in Canada The Rising Tide of Syphilis: Update on Syphilis among Gay, Bisexual, Two-Spirit and other MSM in Canada Wednesday, February 22nd,

More information

Syphilis (TPA) IgG ELISA kit

Syphilis (TPA) IgG ELISA kit Syphilis (TPA) IgG ELISA kit Cat. No.:DEIA2400 Pkg.Size:96T Intended use This product is an enzyme immunoassay for the qualitative detection of IgG antibodies to T. pallidum in serum pecimens. Introduction

More information

Suboptimal Adherence to Repeat Testing Recommendations for Men and Women With Positive Chlamydia Tests in the United States,

Suboptimal Adherence to Repeat Testing Recommendations for Men and Women With Positive Chlamydia Tests in the United States, MAJOR ARTICLE Suboptimal Adherence to Repeat Testing Recommendations for Men and Women With Positive Chlamydia Tests in the United States, 2008 2010 Karen W. Hoover, 1 Guoyu Tao, 1 Melinda B. Nye, 2 and

More information

Summary Guidelines for the Use of Herpes Simplex Virus (HSV) Type 2 Serologies

Summary Guidelines for the Use of Herpes Simplex Virus (HSV) Type 2 Serologies Summary Guidelines for the Use of Herpes Simplex Virus (HSV) Type 2 Serologies Genital herpes is one of the most prevalent sexually transmitted diseases, affecting more than one in five sexually active

More information

Procedure Description Modifier 33 Required? Screening test of visual acuity, quantitative, bilateral No Z Z00.129

Procedure Description Modifier 33 Required? Screening test of visual acuity, quantitative, bilateral No Z Z00.129 Policy Name: Preventive Health Guidelines - Newborns and Children This policy applies only to non-grandfathered plans as defined in the Affordable Care Act section 1251. The following chart contains procedure,

More information

Flagellum in Syphilis

Flagellum in Syphilis JOURNAL OF CLINICAL MICROBIOLOGY, Oct. 1982, p. 608-614 0095-1137/82/100608-07$02.00/0 Copyright 1982, American Society for Microbiology Vol. 16, No. 4 Enzyme-Linked Immunosorbent Assay for Detection of

More information

The performance of rapid plasma reagin (RPR) titer in HIV-negative general paresis after neurosyphilis therapy

The performance of rapid plasma reagin (RPR) titer in HIV-negative general paresis after neurosyphilis therapy Jiang et al. BMC Infectious Diseases (2018) 18:144 https://doi.org/10.1186/s12879-018-3062-4 RESEARCH ARTICLE Open Access The performance of rapid plasma reagin (RR) titer in HIV-negative general paresis

More information

Dr Edward Coughlan. Clinical Director Christchurch Sexual Health,Christchurch

Dr Edward Coughlan. Clinical Director Christchurch Sexual Health,Christchurch Dr Edward Coughlan Clinical Director Christchurch Sexual Health,Christchurch 8:30-9:25 WS #69: The Dreaded Syphilis Strikes Again 9:35-10:30 WS #79: The Dreaded Syphilis Strikes Again (Repeated) Christchurch

More information

Supplementary Online Content

Supplementary Online Content Supplementary Online Content Peters PJ, Westheimer E, Cohen S, et al. Screening yield of HIV antigen/antibody combination and pooled HIV RNA testing for acute HIV infection in a high-prevalence population.

More information

56th Annual Meeting of American Academy of Dermatology. Dermatology and the Information Superhighway

56th Annual Meeting of American Academy of Dermatology. Dermatology and the Information Superhighway REPORTS ON SCIENTIFIC MEETINGS 56th Annual Meeting of American Academy of Dermatology Dermatology and the Information Superhighway reported by Dr. L.Y. Chong Date: 27 February - 4 March 1998 Venue: Orlando,

More information

2/23/2018. Microbiology. Pathogenesis. Congenital Syphilis Epidemiology. Treponema pallidum

2/23/2018. Microbiology. Pathogenesis. Congenital Syphilis Epidemiology. Treponema pallidum Congenital Infections: Syphilis, CMV Anna-Kathryn Burch, M.D. Associate Professor of Pediatrics Medical Director, Pediatric Infectious Diseases Palmetto Health USC University of South Carolina School of

More information