BASIC METABOLIC PANEL

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1 Update 2/12/2018 BASIC METABOLIC PANEL CPT Stability: 3 days at C; 7 days at 2-8 C; > 7 days at -70 C Colorimetric Assay, Rate reaction, ISE Components: BUN, Calcium, Chloride, CO2, Creatinine, Glucose, Potassium, Sodium COMPREHENSIVE METABOLIC PANEL CPT Stability: 3 days at C; 7 days at 2-8 C; > 7 days at -70 C Colorimetric Assay, Rate reaction, ISE Components: Albumin, Alkaline Phosphatase, ALT(SGPT), AST(SGOT), Bilirubin Total, BUN, Calcium, Chloride, CO2, Creatinine, Glucose, Potassium, Sodium ELECTROLYTE PANEL CPT (free from hemolysis) Stability: 7 days C; 7 days 2-8 C; stable (-15)-(-25) C ISE Indirect Components: Chloride, CO2, Potassium, Sodium HEPATIC FUNCTION PANEL CPT Stability: 1 day at C; 7 days at 2-8 C; 6 months at (-15)-(-25) C Components: Albumin, Alkaline Phos, ALT, AST, Bilirubin Direct, Bilirubin Total, Protein Total

2 LIPID PANEL CPT Stability: 7 days at 2-8 C; 30 days at 70 C Enzymatic colorimetric Components: Cholesterol, Triglycerides, HDL, LDL, LDL/HDL Ratio RENAL FUNCTION PANEL CPT Stability: 7 days at C; 7 days at 2-8 C; 3 months at (-15)-(-25) C Components: Albumin, BUN, Calcium, Chloride, CO2, Creatinine, Glucose, Phosphorous, Potassium, Sodium ALBUMIN CPT Stability: 2.5 months at C; 5 months at 2-8 C; 4 months at -20 C ALP (Alkaline Phos) CPT Stability: 7 days at C; 7 days at 2-8 C; 2 months at (-15)-(-25) C ALT (Alanine Aminotransferase) CPT Stability: 3 days at C; 7 days at 2-8 C; > 7 days at -70 C Rate reaction

3 AMMONIA CPT EDTA Plasma Stability: 20 to 30 minutes of venipuncture Enzymatic : Do not use plasma prepared with other anticoagulants. Do not use serum since ammonia can be generated during clotting. Collect blood from stasis-free vein of fasting patient. Smoking should be avoided prior to sampling. Tubes should be filled completely and kept tightly stoppered at all times. Place immediately on ice and centrifuge, preferably at 4 C. AMYLASE CPT Stability: 7 days at C; 1 month at 2-8 C Enzymatic colorimetric assay AST CPT (free from hemolysis) Stability: 1 day at C; 7 days at 2-8 C Rate reaction B12 (VITAMIN B12) CPT (fasting) Stability: Stable for 2 days at 2-8 C, 2 months at -20 C. Freeze once only. Protect from light. BILIRUBIN DIRECT CPT (free from hemolysis and lipemia) Stability: 2 days at C; 7 days at 2-8 C; 6 months at (-15)-(-25) C

4 BILIRUBIN TOTAL CPT Stability: 1 day at C; 7 days at 2-8 C; 6 months at (-15)-(-25) C BUN CPT Stability: 7 days at C; 7 days at 2-8 C; 1 year at (-15)-(-25) C Rate reaction C-REACTIVE PROTEIN hs CPT Stability: 11 days at C; 2 months at 2-8 C; 3 years at (-15)-(-25) C Turbidimetric assay CALCIUM CPT Fresh serum collected in the fasting state Stability: 7 days at C; 3 weeks at 2-8 C; 8 months at (-15)-(-25) C Colorimetric CBC W/AUTO DIFF CPT EDTA-Whole blood Stability: 24 hours at RT; 36 hours at 4 C Automated cell counter Tests include: WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT, RDW, Neutrophils, Lymphocytes, Monocytes, Eosinophils, Basophils, Neutrophils (%), Lymphocytes (%), Monocytes (%), Eosinophils (%), Basophils (%).

5 CBC W/RETIC CPT 85025; EDTA-Whole blood Stability: 24 hours at RT; 36 hours at 4 C Automated cell counter Tests include: WBC, RBC, HGB, HCT, MCV, MCH, MCHC, PLT, RDW, Neutrophils, Lymphocytes, Monocytes, Eosinophils, Basophils, Neutrophils (%), Lymphocytes (%), Monocytes (%), Eosinophils (%), Basophils (%), Reticulocytes (%). CEA CPT Stability: 7 days at 2-8 C, 6 months at -20 C. CHLORIDE CPT (free from hemolysis) Stability: 7 days C; 7 days 2-8 C; stable (-15)-(-25) C ISE Indirect Samples should be separated from the clot or cells promptly after collection. Grossly lipemic specimens should be cleared by ultracentrifugation or ultrafiltration. CHOLESTEROL CPT Seurm Stability: 7 days at C; 7 days at 2-8 C; 3 months at (-15)-(-25) C Enzymatic, colorimetric method CO2 (Bicarbonate) CPT Stability: Several days at 2-8 C when separated from erythrocytes and stored tightly stoppered.

6 CORTISOL CPT Stability: 5 days at 2-8 C, 3 months at -20 C Freeze once only. CPK CPT (free from hemolysis) Stability: 2 days at C; 7 days at 2-8 C; 4 weeks at (-15)-(-25) C Rate reaction CREATININE CPT (free from lipemia) Stability: 7 days at C; 7 days at 2-8 C; 3 months at (-15)-(-25) C DIGOXIN CPT Stability: 2 days at 2-8 C, 6 months at -20 C Freeze once only. ESTRADIOL CPT Stability: 2 days at 2-8 C, 6 months at -20 C Freeze once only.

7 FERRITIN CPT Stability: 7 days at 2-8 C, 12 months at -20 C. FOB (Fecal Occult Blood) Hemosure CPT 82274QW Fecal Collection Tube Stability: 14 hours at C, 6 months at 2-8 C, 1 year -20 C Sandwich dye immunoassay A specimen should not be collected from a patient with the following conditions that may interfere with test results: Menstrual bleeding, Bleeding hemorrhoids, Constipation bleeding, Urinary bleeding. FOLATE CPT (free from hemolysis) Stability: 2 hours at C, 2 days at 2-8 C, 1 month at -20 C Protect from light. Store the samples at 2-8 C if they cannot be measured immediately. FREE PSA CPT 84153; Stability: 5 days at 2-8 C, 3 months at -20 C FREE T3 CPT Stability: 7 days at 2-8 C, 30 days at -20 C; Stability of serum obtained with tubes containing separating gel: 48 hours at 2-8 C

8 FREE T4 CPT Stability: 7 days at 2-8 C, 30 days at -20 C; Stability of serum obtained with tubes containing separating gel: 48 hours at 2-8 C FSH CPT Stability: 14 days at 2-8 C, 6 months at -20 C. ; Stability of serum obtained with separating tubes: 48 hours at 2-8 C GGT CPT Stability: 7 days at C; 7 days at 2-8 C; 1 year at (-15)-(-25) C Enzymatic colorimetric assay GLUCOSE CPT Stability: 8 hours at C; 72 hours at 2-8 C; 3 days at C Enzymatic HCG-BETA CPT Stability: 3 days at 2-8 C, 12 months at -20 C Freeze only once

9 HCG-QUAL CPT Urine or Stability: 8 hours at C, 2-8 C for up to 3 days Instant-View Pregnancy Combo Cassette Test HDL CPT Stability: 7 days at 2-8 C; 30 days at 70 C Enzymatic colorimetric assay H. pylori CPT Stability: 3 days (72hrs) at 2-8 C, QuickVue H.pylori gii HGB & HCT CPT 85018; EDTA-Whole blood Stability: 24 hours at RT; 36 hours at 4 C Automated cell counter HgB A1C CPT Whole blood - EDTA Stability: 3 days at C; 7 days at 2-8 C; 6 months at (-15)-(-25) C Turbidimetric inhibition

10 IRON CPT (free from hemolysis) Stability: 7 days at C; 3 weeks at 2-8 C; several years at (-15)-(-25) C FerroZine LDH CPT (free from hemolysis) Stability: 7 days at C; 4 days at 2-8 C; 6 weeks at -20 C UV-assay LDL CPT Stability: 7 days at 2-8 C; 30 days at -70 C Enzymatic colorimetric assay LH CPT Stability: 14 days at 2-8 C, 6 months at -20 C Freeze only once LIPASE CPT (fresh serum in specimen of choice) Stability: 1 week at C; 1 week at 2-8 C; 1 year at (-15)-(-25) C Enzymatic colorimetric test

11 MAGNESIUM CPT Stability: 3 days at 2-8 C; Prolonged storage -20 C ASI Color Mono II Test MONO CPT Stability: 7 days at C; 7 days at 2-8 C; 1 year at (-15)-(-25) C Colorimetric PARATHYROID HORMONE CPT Stability: 8 hours at C, 2 days at 2-8 C, 6 months at -20 C PARTIAL THROMBOPLASTIN TIME (PTT) CPT Sodium citrated anticoagulated blood. If multiple specimens are collected, the coagulation sample should be the first or second tube collected. Stability: 4 hours at RT; 2 weeks at -20 C; long term storage at -70 C. Clot detection PHOSPHOROUS CPT Stability: 24 hours at C; 4 days at 2-8 C; 1 year at (-15)-(-25) C Colorimetric

12 POTASSIUM CPT (free from hemolysis) Stability: 14 days at C; 14 days at 2-8 C; stable (-15)-(-25) C ISE Indirect Samples should be separated from the clot or cells promptly after collection. Grossly lipemic specimens should be cleared by ultracentrifugation or ultrafiltration. PREALBUMIN CPT Stability: 3 days at 2-8 C; 6 months at (-15)-(-25) C; indefinitely at -70 C Immunotrubidimetric assay PRO BNP CPT Stability: 3 days at C, 6 days at 2-8 C, 24 months at -20 C PROTEIN (TOTAL) CPT Stability: 1 month at 2-8 C; 6 months at (-15)-(-25) C

13 PROTIME (PT) CPT Sodium citrated anticoagulated blood. If multiple specimens are collected, the coagulation sample should be the first or second tube collected. Stability: 24 hours at RT; 36 hours at 4 C; 2 weeks at -20 C; long term storage at -70 C. Clot detection PSA (FREE) CPT 84153; Stability: 5 days at 2-8 C, 3 months at -20 C PSA (TOTAL) CPT 84153; Stability: 5 days at 2-8 C, 6 months at -20 C RETICULOCYTES CPT EDTA-Whole blood Stability: 24 hours at RT; 36 hours at 4 C Automated cell counter SEDIMENTATION RATE, WESTERGREN (ESR) CPT EDTA anticoagulated whole blood Stability: 4 hours at RT; 24 hours at 4 C; do not freeze Sedimat 15

14 SHBG (FREE TESTOSTERONE) CPT Stability: 3 days at 2-8 C, 1 month at -20 C SODIUM CPT (free from hemolysis) Stability: 14 days at C; 14 days at 2-8 C; stable (-15)-(-25) C ISE Indirect Samples should be separated from the clot or cells promptly after collection. Grossly lipemic specimens should be cleared by ultracentrifugation or ultrafiltration. TESTOSTERONE CPT Stability: 1 week at 2-8 C, 6 months at -20 C THYROXINE T4 CPT Stability: 7 days at 2-8 C, 1 month at -20 C TOTAL PROTEIN CPT Stability: 1 month at 2-8 C; 6 months at (-15)-(-25) C

15 TOTAL PSA CPT 84153; Stability: 5 days at 2-8 C, 6 months at -20 C TRIIODOTHYRONINE - T3 CPT Stability: 7 days at 2-8 C, 1 month at -20 C TRIGLYCERIDES CPT Stability: 5-7 days at 2-8 C; 3 months at (-15)-(-25) C; several years at (-60)-(-80) C Enzymatic colorimetric test TROPONIN T CPT Stability: Stable for 24 hours at 2-8 C, 12 months at -20 C TSH CPT Stability: 7 days at 2-8 C, 1 month at -20 C UIBC CPT (free from hemolysis and lipemia) Stability: 4 days at C; 7 days at 2-8 C FerroZine

16 URIC ACID CPT Stability: 5 days at 2-8 C; 6 months at (-15)-(-25) C Enzymatic colorimetric test URINALYSIS CPT Fresh urine. A first morning specimen is preferred but random collections are acceptable. Stability: 2 hours at room temp; If testing is delayed (>2 hour after collection), specimen should be refrigerated (2 to 8 C) for preservation. Dip Stick and Microscopic Exam Tests include: Color, Clarity, Glucose, Bilirubin, Ketone, Specific Gravity, Occult Blood, ph, Protein, Urobilinogen, Nitrate, Leukocytes, Hyaline Casts, WBC, RBC, Crystals, Amorphous Phosphates, Epithelial Cells, Bacteria, and Mucus. URINE MICROSCOPIC CPT Fresh urine. A first morning specimen is preferred but random collections are acceptable. Stability: 2 hours at room temp; If testing is delayed (>2 hour after collection), specimen should be refrigerated (2 to 8 C) for preservation. Microscopic procedure VITAMIN D CPT Stability: 8 hours at C, 4 days at 2-8 C, 24 weeks at -20 C

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