Ocular Toxoplasmosis - A Laboratory View. Chatterton JMW* Evans R. Ho-Yen DO
|
|
- Brittney Perkins
- 5 years ago
- Views:
Transcription
1 Ocular Toxoplasmosis - A Laboratory View Chatterton JMW* Evans R Ho-Yen DO Scottish Toxoplasma Reference Laboratory Microbiology Department Raigmore Hospital Inverness IV UJ * corresponding author
2 Introduction Ocular toxoplasmosis is usually a clinical diagnosis. Laboratory investigations are undertaken to support the clinical diagnosis or help when the clinical presentation is atypical. Ocular toxoplasmosis is usually considered to be due to infection acquired congenitally but may be the result of postnatal infection. Less commonly ocular symptoms may be associated with acute toxoplasma infection. In acute infection antibody levels are raised and specific IgM will be detected. In reactivated infection (usually congenital) antibody levels are often not raised and IgM is not detected. Serological tests in these patients are not diagnostic and are used to confirm previous exposure to Toxoplasma gondii or to exclude the disease. The sensitivity and specificity of the tests used is important. Antibody levels may be low even during episodes of acute ocular disease. At the Scottish Toxoplasma Reference Laboratory a cutoff of iu/ml in the dye test is used. The dye test is the gold standard test and measures all types of immunoglobulin. A positive result is useful in supporting a clinical diagnosis of ocular toxoplasmosis and a negative result is useful in excluding the disease. Toxoplasmosis is a notifiable disease and the Scottish Toxoplasma Reference Laboratory is responsible for reporting cases of toxoplasmosis. Ocular patients with a positive dye test cannot be reported on serological evidence alone. For those patients with ocular symptoms and a positive dye test laboratories are sent questionnaires to forward to the clinician caring for the patient. This report correlates the questionnaire responses and the serological results to assess the laboratory diagnosis of ocular toxoplasmosis. Materials and methods Questionnaires The Scottish Toxoplasma Reference Laboratory receives specimens from throughout Scotland and Northern Ireland. For those patients with ocular symptoms and a positive dye test ( iu/ml) referring laboratories are sent questionnaires to forward to the
3 clinician caring for the patient. Information is requested on the clinical diagnosis and the usefulness of serological testing in confirming the diagnosis. Laboratory tests All serum samples are screened using the in-house IgG enzyme linked immunosorbent assay (EIA), in-house biotin avidin IgM toxoplasma assay and a modification of the Sabin-Feldman dye test. The results of these tests influence the choice of further tests. The tests available include the Abbott AxSym IgM, in-house IgG avidity EIA, IgG Western blotting, and the immunosorbent agglutination assay (ISAGA) for toxoplasma specific IgM, IgA and IgE. Polymerase chain reaction (PCR) techniques are available for use on other specimen types, such as aqueous or vitreous fluid. The questionnaire responses and the laboratory results for patients investigated for ocular toxoplasmosis between April 4 and June 8 were analysed. Results Between April 4 and June 8, 9 patients were investigated for ocular toxoplasmosis. Toxoplasma dye test results < iu/ml were obtained in 9 (5.5%) patients excluding a diagnosis of ocular toxoplasmosis. Toxoplasma dye test results iu/ml were obtained for 5 patients which would support a clinical diagnosis of ocular toxoplasmosis. There was a female to male ratio of.: (9 females and males);.5 % (9/5) were < years old and 59.9% (5/5) were < 5 years old (Table ). Of the 5 patients, 8 (7.%) had a raised dye test result ( 5 iu/ml) and three (.%) had serological evidence of current toxoplasma infection (raised dye test 5 iu/ml, specific IgM and low IgG avidity detected). Toxoplasma gondii DNA was detected by PCR in vitreous fluid samples from two patients.
4 TABLE : Age and dye test result in 5 patients investigated for ocular toxoplasmosis Patients no. (%) Patients no. (%) Age years < (.9) (9.5) (4.6) (8.9) (4.6) (6.) (.5) (.8) (5.5) Dye test result iu/ml (.9) (8.7) (9.9) (5.4) (.8) (7.4) (6.) (.9) (.) Questionnaires were returned on 95/5 patients (7.7%); 57 females and 8 males (.5: ratio). Of these 4 (45.%) were considered clinically to have ocular toxoplasmosis and 5 (54.7%) were considered clinically not to have ocular toxoplasmosis. The ratio of females to males was more pronounced in patients with ocular toxoplasmosis (/;.:) than in those without (7/5;.8:) but this was not significant (P.9, Fisher s exact test, two tailed). Questionnaires were not returned for the three patients with serological evidence of current infection but were returned for the two positive PCR patients. The age and dye test results in the 4 patients with clinical ocular toxoplasmosis and 5 patients considered not to have ocular toxoplasmosis are given in Table. Significantly more patients in the ocular toxoplasmosis group were <5 years old 5/4(8.4%) compared to 5/5 (48%) (P., Fisher s exact test, two tailed). Significantly more patients in the ocular toxoplasmosis group were < years old, /4 (.%) compared to /5 (5.7%) (P.7, Fisher s exact test, two tailed). There was no significant difference in the range of dye test results between patients with and without clinical ocular toxoplasmosis (Table ). Three patients considered not to have ocular toxoplasmosis had raised dye test results 5 iu/ml (Table ). 4
5 TABLE : Age and dye test result in 4 patients with clinical ocular toxoplasmosis and 5 patients considered clinically not to have ocular toxoplasmosis Ocular toxoplasmosis Yes No. patients No No. patients Ocular toxoplasmosis Yes No. patients No No. patients Age years < Dye test result iu/ml Overall, for /4 (76.7%) patients with ocular toxoplasmosis, serological results were considered helpful in making the diagnosis; four of these patients having dye test results of between -4 iu/ml and only two patients having dye test results of 5 iu/ml (Table ). Surprisingly, for /5 (%) of patients without ocular toxoplasmosis the serological results were considered helpful in making the diagnosis (Table ). Of the two patients with ocular toxoplasmosis confirmed by PCR the serological results were considered helpful for one patient but not the other. Both had dye test results of 65 iu/ml with no detectable IgM. 5
6 TABLE : Comparison of the dye test result and whether or not the serological results helped confirm the diagnosis in 4 patients with a clinical diagnosis of ocular toxoplasmosis and 5 patients considered clinically not to have ocular toxoplasmosis Patients Dye test result iu/ml No. with ocular toxoplasmosis Total Serology helpful for Yes No 4 8 diagnosis No. without ocular toxoplasmosis Serology helpful for diagnosis Yes No * *Information on the usefulness of serology was not given for two patients Discussion Over the four-year period, 5 patients with ocular symptoms were found to have positive toxoplasma serology results which would support a clinical diagnosis of ocular toxoplasmosis. Only three patients had evidence of current infection which confirms that most cases are reactivated infection. Of the 95 questionnaires returned, 4 (45.%) confirmed that the patient had ocular toxoplasmosis. If this were reflected in the 5 patients, then 4 cases of ocular toxoplasmosis should have been reported. There were more females than males in the patients with ocular toxoplasmosis (ratio.:). Ocular toxoplasmosis was confirmed in patients aged from < years to > 8 years but there were significantly more in the younger age groups < years and < 5 years. These findings are consistent with our previous study in 999 which found a female to male ratio of.6: in patients with ocular toxoplasmosis and showed that ocular toxoplasmosis was most frequently diagnosed in the age band -. Ocular toxoplasmosis was diagnosed in patients with dye test results from 6
7 5 iu/ml. This confirms the need to use a very sensitive and specific test to avoid false negative results. With screening tests with a threshold of >8 iu/ml, six ocular toxoplasmosis patients would have been missed. Higher dye test results are not necessarily diagnostic and three of the seven patients with dye test results of 5 iu/ml were clinically considered not to have ocular toxoplasmosis. The direct detection of Toxoplasma gondii DNA in ocular fluid by PCR is confirmation of active ocular disease. Toxoplasma DNA was detected in vitreous samples from two patients but experience has shown that these invasive samples are rarely available for testing. It must also be remembered that a negative PCR result does not exclude ocular toxoplasmosis and sensitivities as low as 8% have been reported. 4 This study has shown that for /4 (76.7%) patients with clinical ocular toxoplasmosis the clinicians found the demonstration of specific antibody useful in confirming the diagnosis. This probably reflects how typical the clinical picture is with serology most useful when the presentation is atypical. It is difficult to understand how the demonstration of specific toxoplasma antibodies could help confirm another diagnosis in the /5 patients who did not clinically have ocular toxoplasmosis. It is well recognised that negative toxoplasma serology is useful in excluding ocular toxoplasmosis., Indeed it has been claimed that the only useful serology result is a negative result. The value of demonstrating previous exposure to toxoplasma in the investigation of ocular disease should not be dismissed too readily. This study shows that clinicians can also find positive serology results useful in confirming a diagnosis of ocular toxoplasmosis. Acknowledgements We are grateful to our users for their tremendous cooperation and support. We would like to thank the National Services Division of NHS National Service Scotland and Health Protection Scotland for their continued support. We would like to thank Ms Elena Duff for her assistance with the questionnaires and clinicians and laboratories for kindly completing and returning them. 7
8 References. Joynson DHM and Guy EC. () Laboratory diagnosis of toxoplasma infection. In: Toxoplasmosis: A comprehensive clinical guide. (Ed. DHM Joynson and TG Wregitt) pp96-8. Cambridge University Press.. Ho-Yen DO, Chatterton JMW, Evans R, Ashburn D, Joss AWL. Scottish Toxoplasma Reference Laboratory: users guide. SCIEH Weekly Report ; 4(): 7-7. Available from: Chapman DJ, Ashburn D, Ogston SA and Ho-Yen DO. The relationship between ocular toxoplasmosis and levels of specific toxoplasma antibodies. Epidemiology and Infection 999; : Villard O, Filisetti D, Roch-Deries, Garweg J, Flament,Candolfi E. Comparison of enzyme-linked immunosorbent assay, immunoblotting and PCR for diagnosis of toxoplasmic chorioretinitis. Journal of Clinical Microbiology ; 4: Available from: 8
Annual Report. 1 st April st March Laboratory Service: Toxoplasma gondii. Provider: NHS Highland
Annual Report 1 st April 29-31 st March 21 Laboratory Service: Toxoplasma gondii Provider: NHS Highland Author: Dr D. O. Ho-Yen Director Scottish Toxoplasma Reference Laboratory Date: May 21 1 1. Summary:
More informationEvidence-based diagnosis of toxoplasma infection Evans R, Ho-Yen D O
Evidence-based diagnosis of toxoplasma infection Evans R, Ho-Yen D O Record Status This is a critical abstract of an economic evaluation that meets the criteria for inclusion on NHS EED. Each abstract
More informationArea Laboratory Service
THIS IS A CONTROLLED DOCUMENT. DO NOT COPY Organisational Unit: Title: Ref No: Version No: Date Issued: Author: Authorised by: Review date: Area Laboratory Service Microbiology Scottish Toxoplasma Reference
More informationUKNEQAS Toxoplasma Serology. It is the fault of the kits or is it?
UKNEQAS Toxoplasma Serology It is the fault of the kits or is it? Purpose of the Schemes The Toxoplasma subschemes consist of IgG and IgM and are designed to: Provide information allowing participants
More informationReceived 8 April 1996/Returned for modification 19 June 1996/Accepted 15 July 1996
JOURNAL OF CLINICAL MICROBIOLOGY, Oct. 1996, p. 2526 2530 Vol. 34, No. 10 0095-1137/96/$04.00 0 Copyright 1996, American Society for Microbiology Study of Abbott Toxo IMx System for Detection of Immunoglobulin
More informationAnnual Report April March 2015
Annual Report April 2014 - March 2015 Laboratory Service: Scottish Toxoplasma Reference Laboratory Author: Drs E Watson/R Evans Date : 19 th June 2015 Report due: 19 th June 2015 Table of Contents 1 Summary
More informationUtility of Immunoblotting for Early Diagnosis of Toxoplasmosis Seroconversion in Pregnant Women
CLINICAL AND VACCINE IMMUNOLOGY, Nov. 2011, p. 1908 1912 Vol. 18, No. 11 1556-6811/11/$12.00 doi:10.1128/cvi.05303-11 Copyright 2011, American Society for Microbiology. All Rights Reserved. Utility of
More informationDevelopment of Specific Immunoglobulins G, M, and A Following Primary Toxoplasma gondii Infection in Pregnant Women
JOURNAL OF CLINICAL MICROBIOLOGY, Oct. 1998, p. 2907 2913 Vol. 36, No. 10 0095-1137/98/$04.00 0 Copyright 1998, American Society for Microbiology. All Rights Reserved. Development of Specific Immunoglobulins
More informationFalse-Positive Results in Immunoglobulin M (IgM) Toxoplasma Antibody Tests and Importance of Confirmatory Testing: the Platelia Toxo IgM Test
JOURNAL OF CLINICAL MICROBIOLOGY, Jan. 1997, p. 174 178 Vol. 35, No. 1 0095-1137/97/$04.00 0 Copyright 1997, American Society for Microbiology False-Positive Results in Immunoglobulin M (IgM) Toxoplasma
More informationEvaluation of the Immunoglobulin G Avidity Test for Diagnosis of Toxoplasmic Lymphadenopathy
JOURNAL OF CLINICAL MICROBIOLOGY, Oct. 2004, p. 4627 4631 Vol. 42, No. 10 0095-1137/04/$08.00 0 DOI: 10.1128/JCM.42.10.4627 4631.2004 Copyright 2004, American Society for Microbiology. All Rights Reserved.
More informationEffect of Testing for IgG Avidity in the Diagnosis of Toxoplasma gondii Infection in Pregnant Women: Experience in a US Reference Laboratory
1248 Effect of Testing for IgG Avidity in the Diagnosis of Toxoplasma gondii Infection in Pregnant Women: Experience in a US Reference Laboratory Oliver Liesenfeld, 1,2,3 Jose G. Montoya, 1,2 Sandra Kinney,
More informationImproved Diagnosis of Primary Toxoplasma gondii Infection in Early Pregnancy by Determination of Antitoxoplasma Immunoglobulin G Avidity
JOURNAL OF CLINICAL MICROBIOLOGY, Aug. 1997, p. 1972 1977 Vol. 35, No. 8 0095-1137/97/$04.00 0 Copyright 1997, American Society for Microbiology Improved Diagnosis of Primary Toxoplasma gondii Infection
More informationLecture-7- Hazem Al-Khafaji 2016
TOXOPLASMOSIS Lecture-7- Hazem Al-Khafaji 2016 TOXOPLASMOSIS It is a disease caused by Toxoplasma gondii which is a protozoan parasite that is infects a variety of mammals and birds throughout the world.
More informationagglutination assay (ISAGA) for detecting toxoplasma specific IgM
J Clin Pathol 1989;42:1291-1295 Assessment of immunoglobulin-m immunosorbent agglutination assay (ISAGA) for detecting toxoplasma specific IgM KIM T DUFFY, P J WHARTON, JULIE D JOHNSON, LINDA NEW, R E
More informationTELEFAX. Toxoplasma Serology Laboratory (TSL) DATE: TO: FAX: FROM:
TELEFAX DATE: TO: FAX: FROM: Toxoplasma Serology Laboratory RE: SPECIMEN REQUIREMENTS AND TESTING INFORMATION (effective 1/15/16) THIS TRANSMISSION CONSISTS OF 16 PAGES INCLUDING THIS COVER PAGE. IF THERE
More informationVIDAS Test for Avidity of Toxoplasma-Specific Immunoglobulin G for Confirmatory Testing of Pregnant Women
JOURNAL OF CLINICAL MICROBIOLOGY, July 2002, p. 2504 2508 Vol. 40, No. 7 0095-1137/02/$04.00 0 DOI: 10.1128/JCM.40.7.2504 2508.2002 Copyright 2002, American Society for Microbiology. All Rights Reserved.
More informationFinland. In a preliminary report (10), we have described
JOURNAL OF CLINICAL MICROBIOLOGY, June 1983, p. 988-992 0095-11 37/83/060988-05$02.00/0 Copyright C) 1983, American Society for Microbiology Vol. 17, No. 6 Demonstration of Intraocular Synthesis of Immunoglobulin
More informationArea Laboratory Service
THIS IS A CONTROLLED DOCUMENT. DO NOT COPY Area Laboratory Service Organisational Unit: Title: Ref No: Version No: Date Issued: Author: Authorised by: Review date: Location of this Copy : Microbiology
More informationToxoplasma infection and systemic lupus erythematosus: Analysis of the serological
628 6 Clin Pathol 1993;46:628-632 The Toxoplasma Reference Laboratory, Public Health Laboratory, Leeds I Noel A H Balfour Bacteriology Department, Royal Hallamshire Hospital, Sheffield M H Wilcox Correspondence
More informationFirst Department of Microbiology, School of Medicine, Aristotle University of Thessaloniki, Greece
ORIGINAL ARTICLE 10.1111/j.1469-0691.2005.01193.x Seroprevalence of Toxoplasma gondii in northern Greece during the last 20 years E. Diza, F. Frantzidou, E. Souliou, M. Arvanitidou, G. Gioula and A. Antoniadis
More informationToxoplasma gondii. Jarmila Kliescikova, MD 1. LF UK
Toxoplasma gondii Jarmila Kliescikova, MD 1. LF UK Toxoplasma gondii Apicomplexa, Koccidia Obligate intracellular parasite Distribution: cosmopolite Transmission: alimentary transplacentary (transfusions,
More informationSCREENIG FOR TOXOPLASMOSIS IN SLOVENIA
SCREENIG FOR TOXOPLASMOSIS IN SLOVENIA Mr. Sc. Tjaša Žohar Čretnik, MD Vienna, 7th of april 2015 NATIONAL LABORATORY OF HEALTH, ENVIRONMENT AND FOOD CENTRE FOR MEDICAL MICROBIOLOGY Prvomajska 1 2000 Maribor
More informationImmunoglobulin M Toxoplasma Antibodies
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, JUlY 1994, p. 401-405 Vol. 1, No. 4 1071-412X/94/$04.00+0 Copyright C) 1994, American Society for Microbiology The Vitek Immunodiagnostic Assay for Detection
More informationLaboratory diagnosis of congenital infections
Laboratory diagnosis of congenital infections Laboratory diagnosis of HSV Direct staining Tzanck test Immunostaining HSV isolation Serology PCR Tzanck test Cell scrape from base of the lesion smear on
More informationComparison of the efficiency of two commercial kits ELFA and Western blot in estimating the phase of Toxoplasma gondii infection in pregnant women
Annals of Agricultural and Environmental Medicine 2016, Vol 23, No 4, 570 575 www.aaem.pl ORIGINAL ARTICLE Comparison of the efficiency of two commercial kits ELFA and Western blot in estimating the phase
More informationComparison of immunoblotting, Goldmann-Witmer coefficient and real-time. PCR on aqueous humor for diagnosis of ocular toxoplasmosis.
JCM Accepts, published online ahead of print on 9 April 2008 J. Clin. Microbiol. doi:10.1128/jcm.01900-07 Copyright 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More informationPREDICTIVE VALUE OF LATEX AGGLUTINATION TEST IN SEROLOGICAL SCREENING FOR TOXOPLASMA GONDII
PREDICTIVE VALUE OF LATEX AGGLUTINATION TEST IN SEROLOGICAL SCREENING FOR TOXOPLASMA GONDII Yaowalark Sukthana, Thaiyooth Chintana, Waraporn Supatanapong, Chutatip Siripan, Amorn Lekkla and Rachatawan
More informationtoxoplasmosis Laboratory techniques in the investigation of immunocompromised. Cancer patients, (PCR). Clinical and radiological findings
Genitourin Med 1992;68:55-59 Public Health Laboratory Service, Toxoplasma Reference Laboratory, St George's Hospital, Blackshaw Road, London, SW17 OQT, UK K F Barker R E Holliman Address correspondence
More informationComparative evaluation of AxSYM, VIDAS and VIDIA toxoplasmosis reagent performance in a high seroprevalence Latin American country
Artigo Original / Original Article Comparative evaluation of AxSYM, VIDAS and VIDIA toxoplasmosis reagent performance in a high seroprevalence Latin American country Avaliação comparativa entre o desempenho
More informationModification and Evaluation of Avidity IgG Testing for Differentiating of Toxoplasma gondii Infection in Early Stage of Pregnancy
Original Article Modification and Evaluation of Avidity IgG Testing for Differentiating of Toxoplasma gondii Infection in Early Stage of Pregnancy Mohammad Reza Bonyadi, M.D. 1 *, Parvin Bastani, M.D.
More informationReliable screening for early diagnosis
Elecsys TORCH panel Reliable screening for early diagnosis Toxoplasmosis Rubella HSV CMV Toxoplasmosis The safe and sure approach to Toxo screening Ultrasensitive Toxo IgM optimized to detect all potential
More informationInnovation in Diagnostics. ToRCH. A complete line of kits for an accurate diagnosis INFECTIOUS ID DISEASES
Innovation in Diagnostics ToRCH A complete line of kits for an accurate diagnosis INFECTIOUS ID DISEASES EN TOXOPLASMOSIS Toxoplasmosis is a parasitic disease caused by with the obligate intracellular
More informationA 39 years old HIV-positive black African woman with previously treated cerebral
1 Abstract A 39 years old HIV-positive black African woman with previously treated cerebral toxoplasmosis experienced a foetal intra-uterine death due to congenital toxoplasmosis. This case demonstrates
More informationUNDER REVIEW. UK Standards for Microbiology Investigations. Epstein-Barr Virus Serology
UK Standards for Microbiology Investigations Issued by the Standards Unit, Microbiology Services, PHE Virology V 26 Issue no: 4.1 Issue date: 15.04.14 Page: 1 of 12 Crown copyright 2014 Acknowledgments
More informationSignificance of a positive Toxoplasma Immunoglobulin M test result. in the United States
JCM Accepted Manuscript Posted Online 9 September 2015 J. Clin. Microbiol. doi:10.1128/jcm.01663-15 Copyright 2015, American Society for Microbiology. All Rights Reserved. 1 2 Significance of a positive
More informationImmunoglobulin-A detection and the investigation of clinical toxoplasmosis
J. Med. Microbiol. - Vol. 38 (1993), 286-292 1993 The Pathological Society of Great Britain and Ireland Immunoglobulin-A detection and the investigation of clinical toxoplasmosis BINDYA PATEL, YVONNE YOUNG,
More informationCONTRIBUTIONS OF IMMUNOBLOTTING, REAL-TIME PCR AND THE GOLDMANN-WITMER COEFFICIENT TO THE DIAGNOSIS OF ATYPICAL
JCM Accepts, published online ahead of print on 13 May 2009 J. Clin. Microbiol. doi:10.1128/jcm.00128-09 Copyright 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More informationInt.J.Curr.Microbiol.App.Sci (2018) 7(1):
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 01 (2018) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2018.701.373
More informationDetection of immunoglobulin G antibodies to Toxoplasma gondii: Evaluation of two commercial immunoassay systems
Journal of Microbiology, Immunology and Infection (211) 44, 21e26 available at www.sciencedirect.com journal homepage: www.e-jmii.com ORIGINAL ARTICLE Detection of immunoglobulin G antibodies to Toxoplasma
More informationSimplified Assay for Measuring Toxoplasma gondii Immunoglobulin G Avidity
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, Sept. 2001, p. 904 908 Vol. 8, No. 5 1071-412X/01/$04.00 0 DOI: 10.1128/CDLI.8.5.904 908.2001 Copyright 2001, American Society for Microbiology. All Rights
More informationLaboratory Diagnosis. Dr. M Saraei
Laboratory Diagnosis of toxoplasmosis Dr. M Saraei Qazvin University of Medical Sciences Toxoplasmosis Toxoplasmosis is caused by Toxoplasma gondii, an obligate intracellular protozoan parasite that infects
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Microbiology Department Contact: Dr David Ashburn Zone 3 Raigmore Hospital Tel: +44 (0) 1463 704108 Old Perth Road Fax: +44 (0) 1463 705648
More informationVOL 13, NO 1, JANUREY 2015 SUPPL AL-AZHAR ASSIUT MEDICAL JOURNAL
AL-AZHAR ASSIUT MEDICAL JOURNAL DIAGNOSTIC TESTS TO DETECT RECENT INFECTIONS WITH TOXOPLASMA GONDII IN PREGNANT WOMEN Anwar Hosainy Abo Hashim 1 and Afaf Abdel-Ghany Attya 2 Department of Parasitology,
More informationDiagnostic Methods of HBV and HDV infections
Diagnostic Methods of HBV and HDV infections Zohreh Sharifi,ph.D Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine Hepatitis B-laboratory diagnosis Detection
More informationPerformance of the BioPlex 2200 flow immunoassay (Bio- Rad) in critical cases of serodiagnosis of toxoplasmosis
CVI Accepts, published online ahead of print on 29 January 2014 Clin. Vaccine Immunol. doi:10.1128/cvi.00624-13 Copyright 2014, American Society for Microbiology. All Rights Reserved. 1 2 3 Performance
More informationEvaluation of a new ICT test (LDBIO Diagnostics) to detect toxoplasma IgG. and IgM: comparison with the routine Architect technique.
JCM Accepted Manuscript Posted Online 27 September 2017 J. Clin. Microbiol. doi:10.1128/jcm.01106-17 Copyright 2017 American Society for Microbiology. All Rights Reserved. 1 2 Evaluation of a new ICT test
More informationSerology and International units
Serology and International units L. Grangeot-Keros, National Reference Laboratory for Rubella, Virology Department, A. Béclère Hospital, Clamart, France Detection of rubella-specific IgG antibody Assays
More informationpatient's serum is separated from other serum components by selective absorption of the IgM
JOURNAL OF CLINICAL MICROBIOLOGY, Nov. 1981, p. 486-491 0095-1137/81/1 10486-06$02.00/0 Vol. 14, No. 5 Immunoglobulin M-Immunosorbent Agglutination Assay for Diagnosis of Infectious Diseases: Diagnosis
More informationRecognition of tissue cyst-specific antigens in reactivating toxoplasmosis
J. Med. Microbiol. - Vol. 46 (1997), 587-595 0 1997 The Pathological Society of Great Britain and Ireland SE RO LOG I CAL AND M 0 LECU LAR D IAG N 0s IS Recognition of tissue cyst-specific antigens in
More informationWeekly Influenza Surveillance Report. Week 11
Weekly Influenza Surveillance Report Week 11 Report produced: 22/03/2001 Influenza activity in Ireland For the week ending the 18/03/01, week 11, influenza activity has increased. Sentinel general practices
More informationWales Neonatal Network Guideline
Congenital infection: Diagnosis and management Overview: Infections transmitted and acquired in utero. Most as a result of primary infection of mother during pregnancy, some organisms such as Cytomegalovirus
More informationA stable haemagglutinating antigen for detecting toxoplasma antibodies
J. cli,,. Path., 1972, 25, 762-767 A stable haemagglutinating antigen for detecting toxoplasma antibodies H. THORBURN AND H. WLLAMS From the Microbiology Laboratory, Raigmore Hospital, nverness SYNOPSS
More informationPE1662/E Lyme Disease Action submission of 27 October 2017
PE1662/E Lyme Disease Action submission of 27 October 2017 We support the petitioners calls for action to improve the position with regard to awareness, diagnosis and treatment of Lyme disease. We would
More informationHuman toxoplasmosis in Europe. Parasitology, Paris Descartes University, Cochin Hospital, Paris, France
Human toxoplasmosis in Europe Prof Jean Dupouy-Camet Parasitology, Paris Descartes University, Cochin Hospital, Paris, France Toxoplasma gondii, discovered in 1908, by Nicolle at Institut Pasteur de Tunis,
More informationAutomated microparticle enzyme immunoassays
238 Infectious Diseases and Immunology, Diagnostic Division, Abbott Laboratories, North Chicago, Illinois 60064, USA J W Safford G G Abbott M C Craine R G MacDonald Correspondence to: J W Safford Abbott
More informationالحترمونا من خري الدعاء
الحترمونا من خري الدعاء Instructions for candidates The examination consists of 30 multiple choice questions, each divided into 5 different parts. Each part contains a statement which could be true or
More informationFebruary Monthly Update, Quest Diagnostics Nichols Institute, Valencia
NEW TESTS Please Note: Not all test codes assigned to each assay are listed in the table of contents. Please refer to the complete listing on the page numbers indicated. Test Code Test Name Effective Date
More informationDetection of Toxoplasma Parasitemia by PCR: Does it Correlate with IgG and IgM Antibody Titers?
Detection of Toxoplasma Parasitemia by : Does it Correlate with IgG and IgM Antibody Titers? Behzad Haghpanah 1, Mansoor Salehi 2, Shahram Sadri 1 1 Department of Parasitology and Mycology, 2 Department
More informationResearch Article Decision on conducting HCV Immunoblot and HCV Viral Load Tests Dependent upon the Result of the Screening Tests
IBIMA Publishing Journal of Virology & Microbiology http://www.ibimapublishing.com/journals/jvm/jvm.html Vol. 2013 (2013), Article ID 332501, 7 pages DOI: 10.5171/2013.332501 Research Article Decision
More informationLaboratory Diagnosis of Toxoplasma gondii Infection and Toxoplasmosis
S73 Laboratory Diagnosis of Toxoplasma gondii Infection and Toxoplasmosis Jose G. Montoya Department of Immunology and Infectious Diseases, Research Institute, Palo Alto Medical Foundation, Palo Alto,
More informationParvovirus B19 Mixed Titer Performance Panel PVP201
A SERACARE PANEL PRODUCT INTENDED USE The is a panel of naturally occurring plasma specimens. This panel is intended for use by diagnostic manufacturers and clinical laboratories to evaluate their Parvovirus
More informationSingle donator specimens as advantage in external quality control assessments of infectious diseases
Single donator specimens as advantage in external quality control assessments of infectious diseases MD, PhD Maija Lappalainen and MD Jukka Suni HUSLAB, Department of Virology Labquality Days 10.2.2007
More informationThe solution of the problem of congenital infections as a method to reduce infantile mortality
The solution of the problem of congenital infections as a method to reduce infantile mortality Valeriy V. Vasilyev Professor of the Chair of Infection diseases at North-Western State University n. a. I.I.
More informationVirology Serology Diagnostics Infection Training Dr Alison Watt
Virology Serology Diagnostics Infection Training 2016 Dr Alison Watt Total testing Pathway Lundberg (1981): Brain to Brain loop concept for laboratory testing: Pre-Analytical Pre Post Post Post Analytical
More informationJ07 Titer dynamics, complement fixation test and neutralization tests
avllm0421c (spring 2017) J07 Titer dynamics, complement fixation test and neutralization tests Outline titer, antibody titer dynamics complement, complement fixation reaction neutralization tests 2/35
More informationToxoplasma gondii IgM (Toxo IgM)
DIAGNOSTIC AUTOMATION, INC. 21250 Califa Street, Suite 102 and116, Woodland Hills, CA 91367 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com
More informationRare Presentation of Ocular Toxoplasmosis
Case Report Rare Presentation of Ocular Toxoplasmosis Rakhshandeh Alipanahi MD From Department of Ophthalmology, Nikookari Eye Hospital, Tabriz University of Medical Sciences, Tabriz, Iran. Correspondence:
More informationJOURNAL OF INTERNATIONAL ACADEMIC RESEARCH FOR MULTIDISCIPLINARY Impact Factor 1.393, ISSN: , Volume 2, Issue 2, March 2014
PRELIMINARY EVALUATION OF CHORUS SYSTEM IN COMPARISON WITH COBAS 6000 SYSTEM FOR DETECTION OF ANTI-TOXOPLASMA-IGM ANTIBODIES BLERTA LAZE* ANILA MITRE** *Dept. of Biology, University Ismail Qemali, Vlora,
More informationKnowledge-Based Interpretation of Toxoplasmosis Serology Test Results Including Fuzzy Temporal Concepts
Knowledge-Based Interpretation of Toxoplasmosis Serology Test Results Including Fuzzy Temporal Concepts D. Kopecky, A. Rappelsberger M. Hayde, A.-R. Prusa K.-P. Adlassnig Department of Medical Department
More informationRole of Specific Immunoglobulin E in Diagnosis of Acute Toxoplasma Infection and Toxoplasmosis
JOURNL OF CLINICL MICROBIOLOGY, Nov. 199, p. 2952-2959 95-117/9/112952-8$2./ Copyright 199, merican Society for Microbiology Vol. 1, No. 11 Role of Specific Immunoglobulin in Diagnosis of cute Toxoplasma
More informationUniversity of Perugia, Experimental Medicine and Biochemical Sciences, Perugia, Italy
REVIEW 10.1111/j.1469-0691.2006.01444.x Toxoplasma gondii infection in pregnancy: opportunities and pitfalls of serological diagnosis A. Sensini University of Perugia, Experimental Medicine and Biochemical
More informationAppendix B: Provincial Case Definitions for Reportable Diseases
Infectious Diseases Protocol Appendix B: Provincial Case Definitions for Reportable Diseases Disease: Hemorrhagic fevers caused by: i) Ebola virus and ii) Marburg virus and iii) Other viral causes including
More informationMolecular Markers in Acute and Chronic Phases of Human Toxoplasmosis: Determination of Immunoglobulin G Avidity by Western Blotting
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, May 2000, p. 384 389 Vol. 7, No. 3 1071-412X/00/$04.00 0 Copyright 2000, American Society for Microbiology. All Rights Reserved. Molecular Markers in Acute
More informationClinical Value of Specific Immunoglobulin E Detection by Enzyme-Linked Immunosorbent Assay in Cases of Acquired and Congenital Toxoplasmosis
JOURNAL OF CLINICAL MICROBIOLOGY, Apr. 2003, p. 1681 1686 Vol. 41, No. 4 0095-1137/03/$08.00 0 DOI: 10.1128/JCM.41.4.1681 1686.2003 Copyright 2003, American Society for Microbiology. All Rights Reserved.
More informationToxoplasma IgG ELISA
Instruction Manual Toxoplasma IgG ELISA Enzyme immunoassay based on microtiter plate for the detection and quantitative determination of human IgG antibodies against Toxoplasma gondii in serum and plasma
More informationHIV Serology Quality Assessment Program Summary for Panel HIVS Oct22
National Laboratory for HIV Reference Services National HIV and Retrovirology Laboratories National Microbiology Laboratory Public Health Agency of Canada HIV Serology Quality Assessment Program Summary
More informationHerpesviruses. Tools of diagnosis : what to use and when. Corinne Liesnard Laboratory of Virology Erasme Hospital - ULB
Herpesviruses Tools of diagnosis : what to use and when Corinne Liesnard Laboratory of Virology Erasme Hospital - ULB Evolution of the techniques in the virology lab Techniques : "Classic" methods Ag detection
More informationComplicated viral infections
Complicated viral infections Clinical case discussion Diagnostic dilemmas NSW State Reference Laboratory for HIV St Vincent s Hospital Sydney Diagnostic dilemmas Indeterminate or discordant serology (western
More informationEvaluation of Confounders in Toxoplasmosis Indirect Fluorescent Antibody Assay
Iranian J Parasitol: Vol., No.4, 2, pp.-2 Iranian J Parasitol Tehran University of Medical Sciences Publication http:// tums.ac.ir Open access Journal at http:// ijpa.tums.ac.ir Iranian Society of Parasitology
More informationToxoplasma gondii IgM ELISA Kit
Toxoplasma gondii IgM ELISA Kit Catalog Number KA0226 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle
More informationIdentification of Microbes Lecture: 12
Diagnostic Microbiology Identification of Microbes Lecture: 12 Electron Microscopy 106 virus particles per ml required for visualization, 50,000-60,000 magnification normally used. Viruses may be detected
More informationReceived 24 November 1997/Returned for modification 11 February 1998/Accepted 6 April 1998
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, July 1998, p. 486 490 Vol. 5, No. 4 1071-412X/98/$04.00 0 Copyright 1998, American Society for Microbiology. All Rights Reserved. Seroprevalence of Antibodies
More informationIdentifying false-positive syphilis antibody results using a semi-quantitative
CVI Accepts, published online ahead of print on 20 April 2011 Clin. Vaccine Immunol. doi:10.1128/cvi.05066-11 Copyright 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All
More informationDetection of IgA and low-avidity IgG antibodies for the diagnosis of recent active toxoplasmosis
ORIGINAL ARTICLE Detection of IgA and low-avidity IgG antibodies for the diagnosis of recent active toxoplasmosis Josk Gutikrrex, Marla Rodrkuex, Gonxalo Pie drola and Marla del Carmen Maroto Microbiology
More informationHIV Serology Quality Assessment Program Summary for Panel HIVSER 2017Apr19
National Laboratory for HIV Reference Services National HIV and Retrovirology Laboratories National Microbiology Laboratory Public Health Agency of Canada HIV Serology Quality Assessment Program Summary
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK West of Scotland Specialist Virology Centre New Lister Building Level 5 Glasgow Royal Infirmary 10-16 Alexandra Parade Glasgow G31 2ER Contact:
More informationPappas G. (2009) Int J Parasitol. 39:
Genetic modifications within TLR4and TLR9genes contribute into congenital toxoplasmosis and cytomegalydevelopment Wioletta Wujcicka 1, Jan Wilczyński 1,2, Dorota Nowakowska 1,2 1 Department of Fetal-Maternal
More informationZIKA VIRUS TESTING GUIDANCE, UPDATED 7/20/2016
Section 1: Which patients should be offered testing? SUMMARY OF CATEGORIES OF PATIENTS WHO SHOULD BE TESTED FOR ZIKA 1) Pregnant women who traveled to a Zika-affected area (see link to current list in
More informationUK Standards for Microbiology Investigations
UK Standards for Microbiology Investigations Blood Borne Virus Testing in Dialysis Patients Issued by the Standards Unit, Microbiology Services, PHE Virology V 10 Issue no: 2 Issue date: 27.04.15 Page:
More informationMultiplex Detection of IgM and IgG Class Antibodies to Toxoplasma gondii, Rubella Virus, and Cytomegalovirus Using a Novel Multiplex Flow Immunoassay
CLINICAL AND VACCINE IMMUNOLOGY, Nov. 2010, p. 1734 1738 Vol. 17, No. 11 1556-6811/10/$12.00 doi:10.1128/cvi.00332-10 Copyright 2010, American Society for Microbiology. All Rights Reserved. Multiplex Detection
More informationToxo IgM. MODULAR ANALYTICS E170 cobas e 411 cobas e 602
04618858 190 100 English System information For cobas e 411 analyzer: test number 530 For MODULAR ANALYTICS E170, cobas e 601 and cobas e 602 analyzers: Application Code Number 95 Intended use Immunoassay
More informationPersistence of Immunoglobulin M or Immunoglobulin G Antibody Responses to Borrelia burgdorferi Years after Active Lyme Disease
MAJOR ARTICLE Persistence of Immunoglobulin M or Immunoglobulin G Antibody Responses to Borrelia burgdorferi 10 20 Years after Active Lyme Disease Robert A. Kalish, 1 Gail McHugh, 1 John Granquist, 1 Barry
More informationDetection of acute Toxoplasma gondii infection in pregnant women by IgG avidity and PCR analysis
Tropical Biomedicine 35(4): 908 914 (2018) Detection of acute Toxoplasma gondii infection in pregnant women by IgG avidity and PCR analysis Roozbehani, M. 1, Gharavi, M.J. 2*, Moradi, M. 3 and Razmjou,
More informationToxoplasma gondii IgM ELISA Kit
Toxoplasma gondii IgM ELISA Kit Catalog Number KA0226 96 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle
More informationCongenital CMV infection. Infectious and Tropical Pediatric Division Department of Child Health Medical Faculty, University of Sumatera Utara
Congenital CMV infection Infectious and Tropical Pediatric Division Department of Child Health Medical Faculty, University of Sumatera Utara Congenital CMV infection Approximately 0.15 2% of live births
More informationVarious presentations of herpes simplex retinochoroiditis A case series
Various presentations of herpes simplex retinochoroidits 47 Various presentations of herpes simplex retinochoroiditis A case series M. T. K. Perera 1, T. S. Keragala 1, M. Gamage 2 The Journal of the College
More informationDiagnosis of HIV-1 Infection in Children Younger Than 18 Months in the United States
TECHNICAL REPORT Diagnosis of HIV-1 Infection in Children Younger Than 18 Months in the United States Jennifer S. Read, MD, MS, MPH, DTM&H, and the Committee on Pediatric AIDS ABSTRACT The objectives of
More informationToxoplasmosis in immunocompetent and immunocompromised population of Constanta, Romania
3rd International Conference on Clinical Microbiology & Microbial Genomics September 24-26, 2014 Valencia, Spain Toxoplasmosis in immunocompetent and immunocompromised population of Constanta, Romania
More informationUK Standards for Microbiology Investigations
UK Standards for Microbiology Investigations Epstein-Barr Virus Serology Issued by the Standards Unit, Microbiology Services, PHE Virology V 26 Issue no: 5 Issue date: 03.07.15 Page: 1 of 13 Crown copyright
More information