CLINICAL USE OF CELLULAR SUBPOPULATION ANALYSIS IN BM

Size: px
Start display at page:

Download "CLINICAL USE OF CELLULAR SUBPOPULATION ANALYSIS IN BM"

Transcription

1 CLINICAL USE OF CELLULAR SUBPOPULATION ANALYSIS IN BM CANCER RESEARCH CENTRE, UNIVERSITY AND UNIVERSITY HOSPITAL OF SALAMANCA (SPAIN)( Sao Paulo, 18th of April, 2009

2 IDENTIFICATION OF HPC (I) 1.- In vivo NOD/SCID murine models 350 cgy HSC SSC Anti-human CD In vitro assays HSC Growth Factors BFU-E CFU-GM LTC-IC

3 GENERAL FEATURES OF HPC Hematopoiesis YOLK SAC FETAL LIVER BIRTH BONE MARROW SPLEEN MONTHS OF INTRAUTERINE LIFE YEARS OF LIFE HSC Cytokines HSC ASIMETRIC DIVISION HSC DIFFERENTIATION

4 HEMATOPOIESIS Stem cell Lymphoid precursor T-lymphocytes B-lymphocytes NK-cells Plasmacytoid DC Myeloid precursor Erythroid cells Megakaryocytic cells Neutrophils Monocytes Monocytic DC Eosinophils Basophils Mast cells Myeloid DC

5 MORPHOLOGICAL ANALYSIS OF NORMAL PB

6 IDENTIFICATION OF HPC IMMUNOPHENOTYPING of CD34+ HPC (Backgated on CD45 dim ) Side Scatter Anti-CD34

7 DISTRIBUTION OF CELL POPULATIONS IN NORMAL BM BM cell subset Normal BM (n=12) % CD ( ) % CD34+ or CD (0.6-6) % Erythroid cells (2-9) % Neutrophils (57-80) % Monocytic cells (2-6.5) % Eosinophils (0.8-5) % Basophils (< ) % Mast cells (<0.03) % Plasma cells ( ) % Mature B-cells ( ) % B-cell precursors ( ) % T-lymphocytes (2.6-18) % NK-cells (0.7-5)

8 NORMAL BONE MARROW Eosinophils Neutrophil lineage Monocytic lineage T-SSC CD34+ HPC Nucleated red cells CD45 PERCPCY5,5 -> CD45-PerCP Lymphocytes

9 IMMUNOPHENOTYPIC IDENTIFICATION OF LINEAGE COMMITMENT OF CD34 + BM CELLS Neutrophil precursors TRANSFORMED SSC a CD45 PERCP b b CyMPO PE c CD34 APC TRANSFORMED SSC ntdt FITC B-cell precursors Matarraz S et al. Leukemia 2008

10 GRANULO-MONOCYTIC DIFFERENTIATION IN BM GATE OF CD34 + HPC cmpo + 14% cmpo + CD15 + 5%

11 Neutrophils and Monocytes Follow Different Maturation Pathways CyMPO PE ntdt FITC

12 Neutrophils and Monocytes Follow Different Maturation Pathways Neutrophil Precursor CyMPO PE CyMPO-PE Monocytic precursor ntdt FITC CD35-FITC

13 EARLY ERYTHROID DIFFERENTIATION SSC A HPC CD34 + B-cell precursors NRBC UNGATED BM EVENTS Maturing Neutrophils Eosinophils Basophils pdc Monocytic cells Mature Lymphocytes SSC B UNGATED BM EVENTS CD34 + /CD117 + HPC CD45-PerCP CD34 - B-cell precursors CD34-APC

14 ERYTHROID MATURATION IN NORMAL BM Normal BM Gated CD34+ BM cells Monocytic T-SSC CD64 PE Erythroid PERCPCY5,5 -> CD45-PerCP CD36 FITC

15 IMMUNOPHENOTYPIC IDENTIFICATION OF LINEAGE COMMITMENT OF CD34 + BM CELLS Monocytic f 10 4 pdc e Mast cells h CD64 PE Erythroid CD123 PE Basophil CD117 PE CD36 FITC HLADR FITC HLA-DR FITC Matarraz S et al. Leukemia 2008

16 NORMAL BONE MARROW UNGATED EVENTS A HPC Maturing Neutrophils Eosinophils Basophils pdc CD34 + B-cell precursors Monocytic cells NRBC SSC Mature Lymphocytes CD45-PerCP CD34 - B-cell precursors

17 IMMUNOPHENOTYPE OF CD34+ MYELOID-COMMITTED HPC CELL LINEAGE SSC IMMUNOPHENOTYPE Erythroid Megakaryocytic high Neutrophil Eosinophil Basophil Monocytic Mast cell pdc stable CD36+, CD64-, CD45lo, CD105+ high high low CD61+, CD45lo CyMPO+, CD13hi CyMPO-,, CD15/65+, CyEPO+ CD123hi, HLADRlo, CD117lo CD45hi, CD203c+ stable CyMPO-,, CD64+, DR+, CD117lo low CD117hi, HLADRlo, CD45hi stable CD123hi, HLADRhi, CD36+

18 IMMUNOPHENOTYPE OF LINEAGE COMMITTED CD34+HPC CELL LINEAGE FREQUENCY Erythroid 15% (5%-35%) Megakaryocytic <1% Neutrophil 31% (12-39%) Eosinophil <1% Basophil <1% (<1%-3%) Monocytic 5% (3%-15%) Mast cell <1% (<1%) pdc 6% (1%-15%) 15%) B-Lymphoid 23% (<1%-45%) Total: 81%

19 Normal Maturation of CD34+ Erythroid Precursor Cells in the BM STAGE I STAGE II STAGE III CD34+ CD34+ CD34 lo CD117+ CD117+ CD117+ HLADR+ HLADR lo HLADR lo CD36 lo/+ CD36+ CD36+ CD105- CD105+ CD105+ CD71 lo CD71+ CD71++ CD45 lo CD45 lo CD45 lo

20 EARLY PHENOTYPE OF CD34+ MYELOID-COMMITTED HPC CELL LINEAGE SSC Erythroid stable Megakaryocytic high Neutrophil Eosinophil Basophil Monocytic Mast cell pdc high high low stable low stable EARLIEST ADDITIONAL MARKERS MARKERS CD36+ CD61+ CD105+ CyMPO+, CD15/65, CD64 CyEPO+ CD203c+/ +/CD123hi CD64+, CD15/65 CD35 CD117hi, CyTryp,CD203c CD123hi/CD36+

21 CARACHTERIZATION OF CD34+ HPC: 4-color panel - FITC PE PerCP/Cy5.5 APC Lineage - ccont. - ntdt - ccd3 - CD19 - scont - HLADR - HLADR - CD36 - CD61 - CD71 - EPO ccont. CD45 CD34 - cmpo CD45 CD34 B/neutrophil CD7 CD45 CD34 T ccd79a CD45 CD34 B scont CD45 CD34 - CD117 CD45 CD34 E-N/M-DC/ DC/MC CD123 CD45 CD34 Bas/pDC CD64 CD45 CD34 M/DC DC-Er CD25 CD45 CD34 Meg/T CD105 CD45 CD34 Er EoBP CD45 CD34 Eosinophil Minimum 4-markers requested/needed

22 IMMUNOPHENOTYPIC CHARACTERIZATION OF NORMAL MYELOID MATURATION: CD34- cells

23 NORMAL MATURATION OF NEUTROPHILS

24 PHENOTYPIC CHANGES DURING NORMAL NEUTROPHIL DIFFERENTIATION MYELOBLAST PROMYELOCYTE MYELOCYTE METAMYELOCYT BAND/ NEUTROPHIL CD15 CD13 CD64 MPO CD CD33 CD65 CD CD11b 10 1 CD16 CD10 CD34 HLA-DR CD117 CD54

25 GRANULO-MONOCYTIC DIFFERENTIATION IN BM GATE OF CD34 + HPC cmpo + 14% cmpo + CD15 + 5%

26 Normal Maturation of CD34+ Neutrophil precursor cells in the BM STAGE I STAGE II STAGE III CD34+ CD34+ CD34 lo CD117+ CD117+ CD117+ HLADR+ HLADR + HLADR lo MPO +/++ MPO++ MPO++ CD15/65- CD15/65+ CD15/65++ CD64- CD64- CD64+

27 EARLY NEUTROPHIL VS ERYTHROID DIFFERENTIATION C UNGATED EVENTS D Gated CD117 + and/or CD34 + events SSC CD117 + / CD34 - precursors CD34 + /CD117 + HPC SSC CD117 + / CD34 - Erythroid precursors CD117 + /CD34 - neutrophil precursors CD34 + HPC CD117-PE CD45-PerCP

28 Neutrophil maturation CD16-PE C D 1 6 MON2-L.N.002 CD11b-FITC CD1 1 b FITC 0 APC 4 / CD1 CD3 P E CD34/CD10-APC CD11b FITC -> MON2-L.N.002 CD11b-FITC

29 Neutrophil maturation in normal bone marrow CD13-PE CD11b FITC CD11b-FITC -> MSSA02.005

30 NEUTROPHIL MATURATION Stage I Stage II Stage III Stage IV Stage V MPO Myeloblast Neutrophil 10 1 CD13-APC cmpo MPO-PE PE -> 11b/MPO/DR/ Stage I Stage II Stage III Stage IV Stage V CD CD11b FITC -> 11b/35/45/ CD11b-PE-Cy CD15-FITC -> 15/16/45/10.114

31 PHENOTYPIC CHANGES DURING NORMAL NEUTROPHIL DIFFERENTIATION MYELOBLAST PROMYELOCYTE MYELOCYTE METAMYELOCYT BAND/ NEUTROPHIL CD15 CD13 CD64 MPO CD CD33 CD65 CD CD11b 10 1 CD16 CD10 CD34 HLA-DR CD117 CD54

32 NORMAL MATURATION OF MONOCYTES

33 Normal neutrophil vs monocytic lineage maturation in bone marrow Mono B Lym Blast Mono A Myel Blast HLA-DR FITC RBC C SSC Myel Pro Blast From Loosdrecht et al, Leuk Res, HLA-DR FITC

34 Neutrophils and Monocytes Follow Different Maturation Pathways Neutrophil Precursor Monocytic pathway CyMPO-PE Monocytic precursor CD11c-APC Neutrophil pathway CD35-FITC CD11b-PB CD35/cyMPO/CD34/CD13/CD11c/CD45/CD11b

35 Monocytic differentiation in normal BM CD64-PE CD14-PE MAR FITC -> MON IREM-2 FITC CD36 FITC -> CD36-FITC HLADR CD64 CD36 CD14 IREM2

36 PHENOTYPIC CHANGES DURING MONOCYTIC MATURATION IN NORMAL BM 10 4 MONOBLAST PROMONOCYTE MONOCYTE CD64 Lisozyme CD HLA-DR CD11b CD CD34 CD36 CD14 CD4 IREM-2 MPO 10 1

37 NORMAL MATURATION OF ERYTHROID CELLS

38 ERYTHROID MATURATION IN NORMAL BM Normal BM Gated CD34+ BM cells Monocytic T-SSC CD64 PE Erythroid PERCPCY5,5 -> CD45-PerCP CD36 FITC

39 Normal Maturation of Erythroid Cells in the BM STAGE I STAGE II STAGE III CD34+ CD34+ CD34 lo CD117+ CD117+ CD117+ HLADR+ HLADR lo HLADR lo CD36 lo/+ CD36+ CD36+ CD45 lo CD45 lo CD45 lo

40 EARLY ERYTHROID DIFFERENTIATION SSC A HPC CD34 + B-cell precursors NRBC UNGATED BM EVENTS Maturing Neutrophils Eosinophils Basophils pdc Monocytic cells Mature Lymphocytes SSC B UNGATED BM EVENTS CD34 + /CD117 + HPC CD45-PerCP CD34 - B-cell precursors CD34-APC

41 IMMUNOPHENOTYPE OF LINEAGE COMMITTED CD34+HPC CELL LINEAGE FREQUENCY Erythroid 15% (5%-35%) Megakaryocytic <1% Neutrophil 31% (12-39%) Eosinophil <1% Basophil <1% (<1%-3%) Monocytic 5% (3%-15%) Mast cell <1% (<1%) pdc 6% (1%-15%) 15%) B-Lymphoid 23% (<1%-45%) Total: 81%

42 ERYTHROID DIFFERENTIATION C UNGATED EVENTS D Gated CD117 + and/or CD34 + events SSC CD117 + / CD34 - precursors CD34 + /CD117 + HPC SSC CD117 + / CD34 - Erythroid precursors CD117 + /CD34 - neutrophil precursors CD34 + HPC CD117-PE CD45-PerCP

43 ERYTHROID MATURATION IN NORMAL BM Monocytic f Normal BM CD64 PE Erythroid D Gated CD117 + and/or CD34 + events T-SSC CD36 FITC SSC CD45 PERCPCY5,5 -> CD45-PerCP CD64-PE CD117 + / CD34 - Erythroid precursors CD34 + HPC CD45-PerCP CD36 FITC -> CD36-FITC

44 Normal Maturation of Erythroid Cells in the BM CD71 / CD105 / CD34 / CD117 / CD36 / CD45 CD117-PE CD105-PE CD105-PE CD36-APC CD71-FITC CD36-APC

45 Normal Maturation of Erythroid Cells in the BM STAGE I STAGE II STAGE III STAGE IV CD34+ CD34+ CD34 lo CD34- CD117+ CD117+ CD117+ CD117 lo HLADR+ HLADR lo HLADR lo HLADR- CD36 lo/+ CD36+ CD36+ CD36+ CD105- CD105+ CD105+ CD105 lo/- CD71 lo CD71 lo CD71 hi CD71 hi CD45 lo CD45 lo CD45 lo CD45-

46 PHENOTYPIC CHANGES DURING NORMAL ERYTHROID DIFFERENTIATION STAGE I STAGE II STAGE III STAGE IV Erythrocyte CD CD117 CD CD235a (GLICO A) 10 1 CD34 CD105

47 NORMAL MATURATION OF PLASMACYTOID DENDRITIC CELLS

48 PHENOTYPE OF NORMAL PB DENDRITIC CELLS Ig Receptors C Receptors HLA Molecules CD16 CD32 CD64 CD11c CD35 CD88 HLA DR Myeloid/DC1 Dendritic Cells / Lymphoplasmacytoid /DC2 Dendritic Cells /+++ CD16 + Cells / Adhesion and co-stimulatory molecules Cytokine Receptors Myeloid Ags CD62L CD2 CD4 CD5 CD22 CD86 CD123 CD126 CD13 CD / / /

49 T, NK AND DC DIFFERENTIATION CD34 + HPC CD34 + CD117 + CD135 + CD38 + HLA DR + CD10 - CD45 +/- STROMAL CELLS c-kitl flt-3l Lymph/pDC pdc precursor CD34 + CD13 _/+ CD33 _/+ CD38 + HLA DR + CD10 + CD45RA + T-cell precursor NK/pDC precursor CD44 HI CD5 - CD34 + CD44 LO CD5 + CD34 + B-cell precursor T-cell pdc NK-cell precursor CD34 + CD161 + CD122 + CD56 - precursor CD34+ HLADR HI CD4 + CD40+ CD36+ CD123+++

50 NORMAL BONE MARROW PLASMACYTOID DENDRITIC CELL PRECURSORS CD123-PE CD34 APC HLADR FITC -> HLA DR-FITC CD45 PerCP/Cy5.5 -> CD45-PerCP Cy5.5

51 NORMAL BONE MARROW PLASMACYTOID DENDRITIC CELL PRECURSORS CD123-PE CD34 APC HLADR FITC -> HLA DR-FITC CD45 PerCP/Cy5.5 -> CD45-PerCP Cy Stage I Stage II Stage III

52 IMMUNOPHENOTYPE OF LINEAGE COMMITTED CD34+HPC CELL LINEAGE FREQUENCY Erythroid 15% (5%-35%) Megakaryocytic <1% Neutrophil 31% (12-39%) Eosinophil <1% Basophil <1% (<1%-3%) Monocytic 5% (3%-15%) Mast cell <1% (<1%) pdc 6% (1%-15%) 15%) B-Lymphoid 23% (<1%-45%) Total: 81%

53 PHENOTYPIC CHANGES DURING NORMAL MATURATION OF PLASMACYTOID DENDRITIC CELLS IN BM Antigen-presenting molecules and dendritic cell-related markers 10 5 Cytokine- and Chemokine- receptors 10 5 HLA-ABC CD HLA-DR 10 4 HLA-DP CD117 CD CD197 CD304 CD85j CD303 CD116 CD126 Stage I Stage II Stage III Stage I Stage II Stage III

54 PHENOTYPIC CHANGES DURING NORMAL MATURATION OF PLASMACYTOID DENDRITIC CELLS IN BM Adhesion and co-stimulatory molecules Other molecules CD62L 14% 30% CD34 CD CD45 CD11c+ CD11a CD38 CD CD86 CD11c- CLA CD33 CD45RA CD4 CD2 CD CD99 CD64 CD13 CD22 Stage I Stage II Stage III Stage I Stage II Stage III

55 MATURATION OF NORMAL BM pdc PRECURSORS: PHENOTYPIC CHARACTERISTICS 10 5 Cytokine and chemokine receptors CD CD117 CXCR CD116 CD126 CD126 CCR Stage I Stage II Stage III

56 IMMUNOPHENOTYPE OF NORMAL BM pdc PRECURSORS Specificity Antigen Stage I Stage II Stage III Antigenpresenting molecules DC-associated antigens HLA-ABC /+++ HLA-DR / HLA-DP ++ +d + BDCA BDCA-4 -/ CD85j - +d + Markers absent in all stages: B7-H2 Adhesion molecules Costimulatory molecules Cytokine & Chemokine receptors Myeloid-associated antigens Others CD11a ++ +/++ +/++ CD54 -/+ +d + CD62L ++ - a ++ het - a + het CD2 -/+ -/+ -/+ CD4 -/+d -/+ + CD a + het CD CD116 +d -/+d - CD117 ++/+ -/+d - CD / CD d/- +d/- CXCR4 -/+d +d +d/- CCR7 -/+d - +d CD13 + -/+d - CD33 +/++ +d +d CD22 -/+d - - CD /+ - CD CD45 + +/++ ++ CD45RA + +/++ ++ CLA - a ++ het - a + het - a +d het CD1a, CD1b, CD1c CD45RO CD64 ccd79a, CD79b CD80 CD90 CD99 CD127 CD209 CCR5 CXCR1 CXCR2 TLR4 ctlr9

57 PHENOTYPIC CHANGES DURING NORMAL MATURATION OF PLASMACYTOID DENDRITIC CELLS IN BM Immature precursors STAGE I STAGE II STAGE III CD 123 PE 10 3 CD 36 FITC HLA DR PERCP CD 117 APC BDCA4 PE 10 2 CD 33 APC BDCA 2 PE CD 34 APC 10 1

58 IMMUNOPHENOTYPE OF LINEAGE COMMITTED CD34+HPC CELL LINEAGE FREQUENCY Erythroid 15% (5%-35%) Megakaryocytic <1% Neutrophil 31% (12-39%) Eosinophil <1% Basophil <1% (<1%-3%) Monocytic 5% (3%-15%) Mast cell <1% (<1%) pdc 6% (1%-15%) 15%) B-Lymphoid 23% (<1%-45%) Total: 81%

59 NORMAL MATURATION OF OTHER MINOR MYELOID CELL LINEAGES

60 IMMUNOPHENOTYPIC IDENTIFICATION OF EARLY MAST CELL LINEAGE COMMITMENT OF CD34 + BM CELLS Mast cell precursorss CD117 PE HLA-DR FITC Matarraz S et al. Leukemia 2008

61 PHENOTYPIC CHANGES DURING NORMAL MAST CELL DIFFERENTIATION CD117 cytg4 (Tryptase) CD203c cytb12 (Tryptase) HLA-DR cytg3 (tryptase) CD34 FceRI

62 Conclusiones Schernthaner el al. Allergy, 2005; Galli et al. Nat Immunology, 2005; Marshall, Nat Rev Immunology; Escribano et al. Immunol Allergy Clin N Am, 2006; Orfao et al. Am J Pathol, 1996; Escribano et al. Leuk Res, 2001; Woolhiser et al. Eur J Immunol, 2001; Henz et al. Exp Dermatol, 2001; Stelekati et al. Immunobiology, 2007; Matarraz et al. Leukemia, 2008; Rottem et al. Blood, 1994.

63 PHENOTYPIC CHANGES DURING DIFFERENTION INTO BASOPHILS CD123 CD203 CD34 2D7 FcεRI CD117 HLA-DR

64 OBRIGADO

DIAGNÓSTICO Y CLASIFICACIÓN DE LEUCEMIAS AGUDAS CON LOS PANELES EUROFLOW DIAGNOSTICS IN HEMATO-ONCOLOGY. Clinical symptoms Laboratory

DIAGNÓSTICO Y CLASIFICACIÓN DE LEUCEMIAS AGUDAS CON LOS PANELES EUROFLOW DIAGNOSTICS IN HEMATO-ONCOLOGY. Clinical symptoms Laboratory DIAGNÓSTICO Y CLASIFICACIÓN DE LEUCEMIAS AGUDAS CON LOS PANELES EUROFLOW DIAGNOSIS OF CLONAL HAEMATOLOGICAL DISORDERS Clinical symptoms Laboratory and signs findings Morphology + cytochemistry CANCER RESEARCH

More information

Normal & Leukaemic haematopoiesis. Dr. Liu Te Chih Dept of Haematology / Oncology National University Health Services Singapore

Normal & Leukaemic haematopoiesis. Dr. Liu Te Chih Dept of Haematology / Oncology National University Health Services Singapore Normal & Leukaemic haematopoiesis 2010 Dr. Liu Te Chih Dept of Haematology / Oncology National University Health Services Singapore Use of Immunophenotyping today Lineage assignment Differentiation of

More information

Flow cytometry leukocyte differential : a critical appraisal

Flow cytometry leukocyte differential : a critical appraisal Flow cytometry leukocyte differential : a critical appraisal Francis Lacombe Flow cytometry department University Hospital of Bordeaux, Pessac, France francis.lacombe@chu-bordeaux.fr 2008 HORIBA ABX, All

More information

ACUTE LEUKEMIAS CANCER RESEARCH CENTRE, UNIVERSITY AND UNIVERSITY HOSPITAL OF SALAMANCA (SPAIN)(

ACUTE LEUKEMIAS CANCER RESEARCH CENTRE, UNIVERSITY AND UNIVERSITY HOSPITAL OF SALAMANCA (SPAIN)( ACUTE LEUKEMIAS CANCER RESEARCH CENTRE, UNIVERSITY AND UNIVERSITY HOSPITAL OF SALAMANCA (SPAIN)( Sao Paulo, 18th of April 2009 WHO CLASSIFICATION OF HAEMATOLOGIC MALIGNANCIES Diagnosis and classification

More information

The spectrum of flow cytometry of the bone marrow

The spectrum of flow cytometry of the bone marrow The spectrum of flow cytometry of the bone marrow Anna Porwit Lund University Faculty of Medicine Dept. of Clinical Sciences Div. Oncology and Pathology anna.porwit@med.lu.se Disclosure of speaker s interests

More information

FLOW CYTOMETRIC ANALYSIS OF NORMAL BONE MARROW

FLOW CYTOMETRIC ANALYSIS OF NORMAL BONE MARROW XI International Conference Hematopoiesis Immunology Budapest, June 6-7, 2014 FLO CYTOMETRIC ANALYSIS OF NORMAL BONE MARRO Bruno Brando and Arianna Gatti Hematology Laboratory and Transfusion Center Legnano

More information

The Immune System. A macrophage. ! Functions of the Immune System. ! Types of Immune Responses. ! Organization of the Immune System

The Immune System. A macrophage. ! Functions of the Immune System. ! Types of Immune Responses. ! Organization of the Immune System The Immune System! Functions of the Immune System! Types of Immune Responses! Organization of the Immune System! Innate Defense Mechanisms! Acquired Defense Mechanisms! Applied Immunology A macrophage

More information

Normal Blood and Bone Marrow Populations

Normal Blood and Bone Marrow Populations 4 CHAPTER 4 Normal Blood and Bone Marrow Populations It is essential to have a sound understanding of the nature and immunophenotypic characteristics of the normal cell populations encountered in bone

More information

DISCOVERING ATCC IMMUNOLOGICAL CELLS - MODEL SYSTEMS TO STUDY THE IMMUNE AND CARDIOVASCULAR SYSTEMS

DISCOVERING ATCC IMMUNOLOGICAL CELLS - MODEL SYSTEMS TO STUDY THE IMMUNE AND CARDIOVASCULAR SYSTEMS DISCOVERING ATCC IMMUNOLOGICAL CELLS - MODEL SYSTEMS TO STUDY THE IMMUNE AND CARDIOVASCULAR SYSTEMS James Clinton, Ph.D. Scientist, ATCC February 19, 2015 About ATCC Founded in 1925, ATCC is a non-profit

More information

Hematopoiesis. BHS Liège 27/1/2012. Dr Sonet Anne UCL Mont-Godinne

Hematopoiesis. BHS Liège 27/1/2012. Dr Sonet Anne UCL Mont-Godinne Hematopoiesis BHS Liège 27/1/2012 Dr Sonet Anne UCL Mont-Godinne Hematopoiesis: definition = all the phenomenons to produce blood cells Leukocytes = White Blood Cells Polynuclear = Granulocytes Platelet

More information

VUmc Basispresentatie

VUmc Basispresentatie Clinical diagnostic cytometry Gerrit J Schuurhuis Dept of Hematology VU University Medical Center Amsterdam, Netherlands Use of immunophenotyping at diagnosis to trace residual disease after therapy 1.

More information

Question 1. Kupffer cells, microglial cells and osteoclasts are all examples of what type of immune system cell?

Question 1. Kupffer cells, microglial cells and osteoclasts are all examples of what type of immune system cell? Abbas Chapter 2: Sarah Spriet February 8, 2015 Question 1. Kupffer cells, microglial cells and osteoclasts are all examples of what type of immune system cell? a. Dendritic cells b. Macrophages c. Monocytes

More information

Measuring Dendritic Cells

Measuring Dendritic Cells Measuring Dendritic Cells A.D. Donnenberg, V.S. Donnenberg UNIVERSITY of PITTSBURGH CANCER INSTITUTE CCS Longbeach 10_04 Measuring DC Rare event detection The basics of DC measurement Applications Cancer

More information

Cell sorting: A bridge between morphology and flow cytometry. Pr François Mullier, Pr Bernard Chatelain July 19th, 2013

Cell sorting: A bridge between morphology and flow cytometry. Pr François Mullier, Pr Bernard Chatelain July 19th, 2013 Cell sorting: A bridge between morphology and flow cytometry Pr François Mullier, Pr Bernard Chatelain July 19th, 2013 1 CELL SORTING: PRINCIPLES D.Davies CELL SORTING: PRINCIPLES Aims Efficiency Purity

More information

Standard immunophenotyping of leukemia cells in acute myeloid leukemia (AML)

Standard immunophenotyping of leukemia cells in acute myeloid leukemia (AML) Clinical immunology Standard immunophenotyping of leukemia cells in acute myeloid leukemia (AML) ` JOLANTA WOZNIAK, JOANNA KOPEÆ-SZLÊZAK Department of Haematological Cytobiology, Institut of Haematology

More information

Supplemental Figure 1. IL-3 blockade with Fab CSL362 depletes plasmacytoid dendritic cells (pdcs), but not basophils, at higher doses.

Supplemental Figure 1. IL-3 blockade with Fab CSL362 depletes plasmacytoid dendritic cells (pdcs), but not basophils, at higher doses. Supplemental Figure 1. IL-3 blockade with Fab CSL362 depletes plasmacytoid dendritic cells (pdcs), but not basophils, at higher doses. Percentage of viable (A) pdcs (Sytox Blue-, Lin1-, HLADR+, BDCA2++)

More information

EXAMPLE REPORT ONLY Contact AMS Biotechnology for current donor specific information

EXAMPLE REPORT ONLY Contact AMS Biotechnology for current donor specific information EXAMPLE REPORT ONLY Contact AMS Biotechnology for current donor specific information NAME DIAGNOSIS PROTOCOL OF EVALUATION for Chronic Lymphatic Leukemia (CLL) GENERAL INFORMATION (ALL information required!!)

More information

Morfologia normale e patologica

Morfologia normale e patologica Morfologia normale e patologica Gina Zini Centro di Ricerca ReCAMH Dpt. Ematologia Università Cattolica S. Cuore - Roma EMATOLOGIA DI LABORATORIO: percorsi diagnostici e obiettivi clinici. Milano 11-12

More information

CD34+ Cells: A Comparison of Stem and Progenitor Cells in Cord Blood, Peripheral Blood, and the Bone Marrow

CD34+ Cells: A Comparison of Stem and Progenitor Cells in Cord Blood, Peripheral Blood, and the Bone Marrow White Paper September 2016 CD34+ Cells: A Comparison of Stem and Progenitor Cells in Cord Blood, Peripheral Blood, and the Bone Marrow Lily C. Trajman, PhD Introduction: Hematopoietic Stem Cells (HSCs)

More information

2007 Workshop of Society for Hematopathology & European Association for Hematopathology Indianapolis, IN, USA Case # 228

2007 Workshop of Society for Hematopathology & European Association for Hematopathology Indianapolis, IN, USA Case # 228 2007 Workshop of Society for Hematopathology & European Association for Hematopathology Indianapolis, IN, USA Case # 228 Vishnu V. B Reddy, MD University of Alabama at Birmingham Birmingham, AL USA 11/03/07

More information

Nature Immunology: doi: /ni Supplementary Figure 1. Examples of staining for each antibody used for the mass cytometry analysis.

Nature Immunology: doi: /ni Supplementary Figure 1. Examples of staining for each antibody used for the mass cytometry analysis. Supplementary Figure 1 Examples of staining for each antibody used for the mass cytometry analysis. To illustrate the functionality of each antibody probe, representative plots illustrating the expected

More information

Comprehensive evaluation of human immune system reconstitution in NSG. and NSG -SGM3 mouse models toward the development of a novel ONCO-HU

Comprehensive evaluation of human immune system reconstitution in NSG. and NSG -SGM3 mouse models toward the development of a novel ONCO-HU Comprehensive evaluation of human immune system reconstitution in NSG and NSG -SGM3 mouse models toward the development of a novel ONCO-HU xenograft model Aaron Middlebrook, 1 Eileen Snowden, 2 Warren

More information

BD Flow Cytometry Reagents Multicolor Panels Designed for Optimal Resolution with the BD LSRFortessa X-20 Cell Analyzer

BD Flow Cytometry Reagents Multicolor Panels Designed for Optimal Resolution with the BD LSRFortessa X-20 Cell Analyzer Multicolor Panels Designed for Optimal Resolution with the BD LSRFortessa X-2 Cell Analyzer Proper multicolor panel design takes into account fluorochrome brightness, antigen density, co-expression, and

More information

23/10/2017. Analysis of bone marrow for MDSrelated. Approach used at Hematopathology, Lund

23/10/2017. Analysis of bone marrow for MDSrelated. Approach used at Hematopathology, Lund Approach used at Hematopathology, Lund Analysis of bone marrow for MDSrelated aberrancies According to International/ELN Flow Cytometry Working Group (IMDSFlow) Anna Porwit Lund, Sweden 1. new patients

More information

Welcome. Welcome. Emerging Technologies in Flow Cytometry

Welcome. Welcome. Emerging Technologies in Flow Cytometry Emerging Technologies in Flow Cytometry Dr. William Dittman December 11, 2012 You may download a copy of the handout by clicking on the handout icon, located in the upper right hand corner of your screen

More information

Ron Lee, MD Hematopathologist, Esoterix Pathology Practice Group, PC Brentwood, TN Office Cellular

Ron Lee, MD Hematopathologist, Esoterix Pathology Practice Group, PC Brentwood, TN Office Cellular Ron Lee, MD Hematopathologist, Esoterix Pathology Practice Group, PC Brentwood, TN Office 615-377-7145 Cellular 615-715-9599 Email ron.lee@labcorp.com Disclosure In the past 12 months, I have not had a

More information

Clinical question. Screening tube. Diagnostic panel MRD. Clinical question

Clinical question. Screening tube. Diagnostic panel MRD. Clinical question OW CYTOMETRY UPDATES IN LYMPHOPROLIFERATIVE DISORDERS CANCER RESEARCH CENTER IBSAL UNIVERSITY & UNIVERSITY HOSPITAL, SALAMANCA (SPAIN) DISCLOSURES The EuroFlow Scientific Consortium Iamco-chairof receives

More information

Getting to the root of Cancer

Getting to the root of Cancer Cancer Stem Cells: Getting to the root of Cancer Dominique Bonnet, Ph.D Senior Group Leader, Haematopoietic Stem Cell Laboratory Cancer Research UK, London Research Institute Venice, Sept 2009 Overview

More information

Myelodysplastic scoring system with flow cytometry. G Detry B Husson

Myelodysplastic scoring system with flow cytometry. G Detry B Husson Myelodysplastic scoring system with flow cytometry G Detry B Husson Myelodysplastic syndroms Clonal haematopoietic stem cell disease characterized by dysplasia in one or more of the myeloid cell lines

More information

HISTOLOGY VIRTUAL LABORATORY BLOOD AND LYMPHATICS SYSTEM

HISTOLOGY VIRTUAL LABORATORY BLOOD AND LYMPHATICS SYSTEM HISTOLOGY VIRTUAL LABORATORY BLOOD AND LYMPHATICS SYSTEM Login: http://histopath.westernu.edu Histology Atlas AND Virtual Histology links. I. HEMATOLOGY - PERIPHERAL BLOOD Purpose: To be able to identify

More information

Ordering Physician CLIENT,CLIENT. Collected REVISED REPORT

Ordering Physician CLIENT,CLIENT. Collected REVISED REPORT HPWET Hematopathology Consultation, MML Embed Client Hematopathology Consult REVISED INAL DIAGNOSIS Interpretation Peripheral blood, bone marrow aspirate and biopsies, bilateral iliac crests: 1. Normocellular

More information

Introduction to Haematology. Prof Roger Pool Department of Haematology University of Pretoria

Introduction to Haematology. Prof Roger Pool Department of Haematology University of Pretoria Introduction to Haematology Prof Roger Pool Department of Haematology University of Pretoria Suggested reading Haematology at a Glance Atul Mehta & Victor Hoffbrand Second Edition Published by Blackwell

More information

FLOW CYTOMETRY PRINCIPLES AND PRACTICE. Toby Eyre Consultant Haematologist Oxford University Hospitals NHS Foundation Trust June 2018

FLOW CYTOMETRY PRINCIPLES AND PRACTICE. Toby Eyre Consultant Haematologist Oxford University Hospitals NHS Foundation Trust June 2018 FLOW CYTOMETRY PRINCIPLES AND PRACTICE Toby Eyre Consultant Haematologist Oxford University Hospitals NHS Foundation Trust June 2018 Aims and Objectives Principles of flow cytometry Preparation Steps involved

More information

VETERINARY HEMATOLOGY ATLAS OF COMMON DOMESTIC AND NON-DOMESTIC SPECIES COPYRIGHTED MATERIAL SECOND EDITION

VETERINARY HEMATOLOGY ATLAS OF COMMON DOMESTIC AND NON-DOMESTIC SPECIES COPYRIGHTED MATERIAL SECOND EDITION VETERINARY HEMATOLOGY ATLAS OF COMMON DOMESTIC AND NON-DOMESTIC SPECIES SECOND EDITION COPYRIGHTED MATERIAL CHAPTER ONE HEMATOPOIESIS GENERAL FEATURES All blood cells have a finite life span, but in normal

More information

Myeloproliferative Disorders - D Savage - 9 Jan 2002

Myeloproliferative Disorders - D Savage - 9 Jan 2002 Disease Usual phenotype acute leukemia precursor chronic leukemia low grade lymphoma myeloma differentiated Total WBC > 60 leukemoid reaction acute leukemia Blast Pro Myel Meta Band Seg Lymph 0 0 0 2

More information

ADx Bone Marrow Report. Patient Information Referring Physician Specimen Information

ADx Bone Marrow Report. Patient Information Referring Physician Specimen Information ADx Bone Marrow Report Patient Information Referring Physician Specimen Information Patient Name: Specimen: Bone Marrow Site: Left iliac Physician: Accession #: ID#: Reported: 08/19/2014 - CHRONIC MYELOGENOUS

More information

Flow Cytometry. What is flow cytometry?

Flow Cytometry. What is flow cytometry? Flow Cytometry Flow Cytometry What is flow cytometry? Flow cytometry is a popular laser-based technology to analyze the characteristics of cells or particles. It is predominantly used to measure fluorescence

More information

Hematopoiesis. Hematopoiesis. Hematopoiesis

Hematopoiesis. Hematopoiesis. Hematopoiesis Chapter. Cells and Organs of the Immune System Hematopoiesis Hematopoiesis- formation and development of WBC and RBC bone marrow. Hematopoietic stem cell- give rise to any blood cells (constant number,

More information

a Beckman Coulter Life Sciences: White Paper

a Beckman Coulter Life Sciences: White Paper a Beckman Coulter Life Sciences: White Paper An 8-color DuraClone IM panel for detection of Human blood dendritic cells by flow cytometry Nathalie Dupas 1, Snehita Sattiraju 2, Neha Girish 2, Murthy Pendyala

More information

Case #1. 65 yo man with no prior history presented with leukocytosis and circulating blasts: Bone marrow biopsy was performed

Case #1. 65 yo man with no prior history presented with leukocytosis and circulating blasts: Bone marrow biopsy was performed Case #1 65 yo man with no prior history presented with leukocytosis and circulating blasts: WBC 187.4K/uL ; Hgb 10.0gm/dL; Platelet 68K/uL Neutrophil % 25.0% Lymphocyte % 38.0% Monocyte % 12.0% Metamyelocyte

More information

4. TEXTBOOK: ABUL K. ABBAS. ANDREW H. LICHTMAN. CELLULAR AND MOLECULAR IMMUNOLOGY. 5 TH EDITION. Chapter 2. pg

4. TEXTBOOK: ABUL K. ABBAS. ANDREW H. LICHTMAN. CELLULAR AND MOLECULAR IMMUNOLOGY. 5 TH EDITION. Chapter 2. pg LECTURE: 03 Title: CELLS INVOLVED IN THE IMMUNE RESPONSE LEARNING OBJECTIVES: The student should be able to: Identify the organs where the process of the blood formation occurs. Identify the main cell

More information

Extramedullary precursor T-lymphoblastic transformation of CML at presentation

Extramedullary precursor T-lymphoblastic transformation of CML at presentation Extramedullary precursor T-lymphoblastic transformation of CML at presentation Neerja Vajpayee, Constance Stein, Bernard Poeisz & Robert E. Hutchison Clinical History 30 year old man presented to the emergency

More information

MACROPHAGE "MONOCYTES" SURFACE RECEPTORS

MACROPHAGE MONOCYTES SURFACE RECEPTORS LECTURE: 13 Title: MACROPHAGE "MONOCYTES" SURFACE RECEPTORS LEARNING OBJECTIVES: The student should be able to: Describe the blood monocytes (size, and shape of nucleus). Enumerate some of the monocytes

More information

Participants Identification No. % Evaluation. Mitotic figure Educational Erythrocyte precursor, abnormal/

Participants Identification No. % Evaluation. Mitotic figure Educational Erythrocyte precursor, abnormal/ Cell Identification BMD-09 Participants Identification No. % Evaluation Mitotic figure 233 96.7 Educational Erythrocyte precursor, abnormal/ 4 1.7 Educational dysplastic nuclear features Erythrocyte precursor

More information

An adequate diagnosis is the first goal towards

An adequate diagnosis is the first goal towards Acute Leukemias New methodologic approaches for immunophenotyping acute leukemias trends in hematology haematologica 2001; 86:675-692 http://www.haematologica.it/2001_07/0675.htm GIUSEPPE BASSO, BARBARA

More information

Formation of Blood Cells

Formation of Blood Cells Hematopoiesis Lecture Objectives Name organs responsible for hematopoiesis in the fetus. List the developmental stages of hematopoiesis both prenatally and postnatally. Outline the major steps of post

More information

Towards the study of bone marrow DTC in breast and ovarian cancer

Towards the study of bone marrow DTC in breast and ovarian cancer Towards the study of bone marrow DTC in breast and ovarian cancer Nikolay Tupitsyn FSBI N.N.Blokhin Russian Cancer Research Center, the Russian Academy of Medical Sciences MOSCOW, RUSSIA HAEMATOPOIESIS

More information

Flow Cytomety Immunophenotyping For Myelodysplastic Syndromes. Sa A.Wang, MD Dept. of Hematopathology UT MD Anderson Cancer Center Houston, TX

Flow Cytomety Immunophenotyping For Myelodysplastic Syndromes. Sa A.Wang, MD Dept. of Hematopathology UT MD Anderson Cancer Center Houston, TX Flow Cytomety Immunophenotyping For Myelodysplastic Syndromes Sa A.Wang, MD Dept. of Hematopathology UT MD Anderson Cancer Center Houston, TX Myelodysplastic Syndromes Definition: A group of heterogeneous

More information

BLOOD PHYSIOLOGY. White Blood Cells (WBC) Dr Nervana Mostafa

BLOOD PHYSIOLOGY. White Blood Cells (WBC) Dr Nervana Mostafa BLOOD PHYSIOLOGY White Blood Cells (WBC) Dr Nervana Mostafa 1 Lecture content. 1 Eosinophils and Basophilophils formation, maturation and function. 2. 3. 4. 5 Monocytes and macrophage formation, maturation

More information

Collected: , PM Sent: , PM Received: , PM Preliminary: , PM. Notification Status: COMPREHENSIVE DIAGNOSIS

Collected: , PM Sent: , PM Received: , PM Preliminary: , PM. Notification Status: COMPREHENSIVE DIAGNOSIS PATIENT Name:HIPAA, Compliant DOB: 03-25-1945 (60 yr) ID#: xxx-xx-0000 Sex: M Tel: xxx-xxx-xxxx SPECIMEN Your No:WS05-xxxx Case No:C05-00xxx Req. No:Txxxxx Collected: 06-08-05, PM Sent: 06-09-05, PM Received:

More information

sequences of a styx mutant reveals a T to A transversion in the donor splice site of intron 5

sequences of a styx mutant reveals a T to A transversion in the donor splice site of intron 5 sfigure 1 Styx mutant mice recapitulate the phenotype of SHIP -/- mice. (A) Analysis of the genomic sequences of a styx mutant reveals a T to A transversion in the donor splice site of intron 5 (GTAAC

More information

Notes for the 2 nd histology lab

Notes for the 2 nd histology lab Notes for the 2 nd histology lab Note : Please refer to the slides and see the morphological characteristics of each cell, as the practical exam will be in the form of figures. SLIDE #2 Erythropoiesis

More information

Hua Tang, Weiping Cao, Sudhir Pai Kasturi, Rajesh Ravindran, Helder I Nakaya, Kousik

Hua Tang, Weiping Cao, Sudhir Pai Kasturi, Rajesh Ravindran, Helder I Nakaya, Kousik SUPPLEMENTARY FIGURES 1-19 T H 2 response to cysteine-proteases requires dendritic cell-basophil cooperation via ROS mediated signaling Hua Tang, Weiping Cao, Sudhir Pai Kasturi, Rajesh Ravindran, Helder

More information

Childhood hematopoiesis and hematological features. Yongmin Tang Dept. Hematology-oncology Chidlren s Hospital Zhejiang University School of medicine

Childhood hematopoiesis and hematological features. Yongmin Tang Dept. Hematology-oncology Chidlren s Hospital Zhejiang University School of medicine Childhood hematopoiesis and hematological features Yongmin Tang Dept. Hematology-oncology Chidlren s Hospital Zhejiang University School of medicine Questions: How much have you known about our hematopoietic

More information

Fluorochrome Panel 1 Panel 2 Panel 3 Panel 4 Panel 5 CTLA-4 CTLA-4 CD15 CD3 FITC. Bio) PD-1 (MIH4, BD) ICOS (C398.4A, Biolegend) PD-L1 (MIH1, BD)

Fluorochrome Panel 1 Panel 2 Panel 3 Panel 4 Panel 5 CTLA-4 CTLA-4 CD15 CD3 FITC. Bio) PD-1 (MIH4, BD) ICOS (C398.4A, Biolegend) PD-L1 (MIH1, BD) Additional file : Table S. Antibodies used for panel stain to identify peripheral immune cell subsets. Panel : PD- signaling; Panel : CD + T cells, CD + T cells, B cells; Panel : Tregs; Panel :, -T, cdc,

More information

ACTIVATION AND EFFECTOR FUNCTIONS OF CELL-MEDIATED IMMUNITY AND NK CELLS. Choompone Sakonwasun, MD (Hons), FRCPT

ACTIVATION AND EFFECTOR FUNCTIONS OF CELL-MEDIATED IMMUNITY AND NK CELLS. Choompone Sakonwasun, MD (Hons), FRCPT ACTIVATION AND EFFECTOR FUNCTIONS OF CELL-MEDIATED IMMUNITY AND NK CELLS Choompone Sakonwasun, MD (Hons), FRCPT Types of Adaptive Immunity Types of T Cell-mediated Immune Reactions CTLs = cytotoxic T lymphocytes

More information

IMMUNOLOGY. Source, Isolate, Culture, And Analyze Immune Cells. Scientists Helping Scientists

IMMUNOLOGY. Source, Isolate, Culture, And Analyze Immune Cells. Scientists Helping Scientists IMMUNOLOGY Source, Isolate, Culture, And Analyze Immune Cells Scientists Helping Scientists WWW.STEMCELL.COM TABLE OF CONTENTS Tools For Your Immunology Research 4 Primary Cells: It All Starts with The

More information

ICCS e-newsletter CSI Case Laura Sánchez-Muñoz, Jose Mario T. Morgado, Cristina Teodosio

ICCS e-newsletter CSI Case Laura Sánchez-Muñoz, Jose Mario T. Morgado, Cristina Teodosio ICCS e-newsletter CSI Case Laura Sánchez-Muñoz, Jose Mario T. Morgado, Cristina Teodosio Instituto de Estudios de Mastocitosis and Centro de Investigación del Cáncer Spanish Network on Mastocytosis Spain

More information

Expression of CD117 and CD11b in Bone Marrow Can Differentiate Acute Promyelocytic Leukemia From Recovering Benign Myeloid Proliferation

Expression of CD117 and CD11b in Bone Marrow Can Differentiate Acute Promyelocytic Leukemia From Recovering Benign Myeloid Proliferation Hematopathology / CD117 AND CD11B IN PROMYELOCYTIC BONE MARROW Expression of CD117 and CD11b in Bone Marrow Can Differentiate Acute Promyelocytic Leukemia From Recovering Benign Myeloid Proliferation Edgar

More information

IMMUNOLOGICAL MEMORY. CD4 T Follicular Helper Cells. Memory CD8 T Cell Differentiation

IMMUNOLOGICAL MEMORY. CD4 T Follicular Helper Cells. Memory CD8 T Cell Differentiation IMMUNOLOGICAL MEMORY CD4 T Follicular Helper Cells Memory CD8 T Cell Differentiation CD4 T Cell Differentiation Bcl-6 T-bet GATA-3 ROR t Foxp3 CD4 T follicular helper (Tfh) cells FUNCTION Provide essential

More information

Case Presentation. Pei Lin, M. D.

Case Presentation. Pei Lin, M. D. Case Presentation Pei Lin, M. D. History A 26 yr man reports a history of numerous skin and upper respiratory infections as a child, including lymphadenitis and meningitis. In March 2013 during a preoperative

More information

By Dr. Mohamed Saad Daoud

By Dr. Mohamed Saad Daoud By Dr. Mohamed Saad Daoud Part I Introduction Types of White Blood Cells Genesis of the White Blood Cells Life Span of the White Blood Cells Dr. Mohamed Saad Daoud 2 Leucocytes Introduction: Infectious

More information

Juvenile Myelomonocytic Leukemia (JMML)

Juvenile Myelomonocytic Leukemia (JMML) Juvenile Myelomonocytic Leukemia (JMML) JMML: Definition Monoclonal hematopoietic disorder of childhood characterized by proliferation of the granulocytic and monocytic lineages Erythroid and megakaryocytic

More information

DURACLONE IF BE CERTAIN ABOUT THE RESPONSE. l res. a il n c n. For Research Use Only - Not for use in Diagnostic procedures

DURACLONE IF BE CERTAIN ABOUT THE RESPONSE. l res. a il n c n. For Research Use Only - Not for use in Diagnostic procedures DURACLONE IF earch tria l res lc a om ic il n c n nio pa Yo ur BE CERTAIN ABOUT THE RESPONSE For Research Use Only - Not for use in Diagnostic procedures BE CERTAIN ABOUT THE RESPONSE The sensitive and

More information

Naive, memory and regulatory T lymphocytes populations analysis

Naive, memory and regulatory T lymphocytes populations analysis Naive, memory and regulatory T lymphocytes populations analysis Jaen Olivier, PhD ojaen@beckmancoulter.com Cellular Analysis application specialist Beckman Coulter France Introduction Flow cytometric analysis

More information

General Overview of Immunology. Kimberly S. Schluns, Ph.D. Associate Professor Department of Immunology UT MD Anderson Cancer Center

General Overview of Immunology. Kimberly S. Schluns, Ph.D. Associate Professor Department of Immunology UT MD Anderson Cancer Center General Overview of Immunology Kimberly S. Schluns, Ph.D. Associate Professor Department of Immunology UT MD Anderson Cancer Center Objectives Describe differences between innate and adaptive immune responses

More information

Prepared by Cyrus H. Nozad, MD, University of Tennessee and John Seyerle, MD, Ohio State University

Prepared by Cyrus H. Nozad, MD, University of Tennessee and John Seyerle, MD, Ohio State University Allergy and Immunology Review Corner: Chapter 21 of Middleton s Allergy Principles and Practice, Seventh Edition, edited by N. Franklin Adkinson, et al. Chapter 21: Antigen-Presenting Dendritic Cells (Pages

More information

Hematopathology Case Study

Hematopathology Case Study Hematopathology Case Study AMP Outreach Course 2009 AMP Annual Meeting John Greg Howe Ph.D. Department of Laboratory Medicine Yale University School of Medicine November 19, 2009 HISTORY Case History An

More information

Hematopoie)c System. Kris)ne Kra2s, M.D.

Hematopoie)c System. Kris)ne Kra2s, M.D. Hematopoie)c System Kris)ne Kra2s, M.D. Hematopoie)c System Lecture Objec)ves Describe the developmental stages of erythropoiesis. Describe the developmental stages of granulopoiesis. Describe the differences

More information

A Look Into the Determination of Cell Morphology in Hematology in the 21 st Century. Ramon Simon-Lopez, MD Global Scientific Director Beckman Coulter

A Look Into the Determination of Cell Morphology in Hematology in the 21 st Century. Ramon Simon-Lopez, MD Global Scientific Director Beckman Coulter A Look Into the Determination of Cell Morphology in Hematology in the 21 st Century Ramon Simon-Lopez, MD Global Scientific Director Beckman Coulter Is cell morphology important? AML M7 CLL CD5 CD19 NHL

More information

G-CSF-primed autologous and allogeneic bone marrow for transplantation in clinical oncology. Cell content and immunological characteristics

G-CSF-primed autologous and allogeneic bone marrow for transplantation in clinical oncology. Cell content and immunological characteristics Journal of Physics: Conference Series PAPER OPEN ACCESS G-CSF-primed autologous and allogeneic bone marrow for transplantation in clinical oncology. Cell content and immunological characteristics To cite

More information

Lymphoid architecture & Leukocyte recirculation. Thursday Jan 26th, 2017

Lymphoid architecture & Leukocyte recirculation. Thursday Jan 26th, 2017 Lymphoid architecture & Leukocyte recirculation Thursday Jan 26th, 2017 Topics The life of immune cells Where are they born? Where are they educated? Where do they function? How do they get there? The

More information

Hematology 101. Blanche P Alter, MD, MPH, FAAP Clinical Genetics Branch Division of Cancer Epidemiology and Genetics Bethesda, MD

Hematology 101. Blanche P Alter, MD, MPH, FAAP Clinical Genetics Branch Division of Cancer Epidemiology and Genetics Bethesda, MD Hematology 101 Blanche P Alter, MD, MPH, FAAP Clinical Genetics Branch Division of Cancer Epidemiology and Genetics Bethesda, MD Hematocrits Plasma White cells Red cells Normal, Hemorrhage, IDA, Leukemia,

More information

Recommended Protocols for Phospho Protein Detection in Human Cells

Recommended Protocols for Phospho Protein Detection in Human Cells Recommended Protocols for Phospho Protein Detection in Human Cells Depending on subcellular localization of the phospho protein of interest, as well as epitope susceptibility to cell fixing and permeabilizing

More information

Hematology 101. Rachid Baz, M.D. 5/16/2014

Hematology 101. Rachid Baz, M.D. 5/16/2014 Hematology 101 Rachid Baz, M.D. 5/16/2014 Florida 101 Epidemiology Estimated prevalence 8,000 individuals in U.S (compare with 80,000 MM patients) Annual age adjusted incidence 3-8/million-year 1 More

More information

بسم هللا الرحمن الرحيم

بسم هللا الرحمن الرحيم بسم هللا الرحمن الرحيم WBCs disorders *Slide 2: - we will focus on the disorders that are related to the # of WBCs - in children the # of lymphocyte is more than it in adults,sometimes more than neutrophils

More information

Allergy and Immunology Review Corner: Chapter 1 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti.

Allergy and Immunology Review Corner: Chapter 1 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti. Allergy and Immunology Review Corner: Chapter 1 of Immunology IV: Clinical Applications in Health and Disease, by Joseph A. Bellanti. Chapter 1: Overview of Immunology Prepared by David Scott, MD, Scripps

More information

Blood Cell Identification Graded

Blood Cell Identification Graded BCP-21 Blood Cell Identification Graded Case History The patient is a 37-year-old female with a history of multiple sickle cell crises. She now presents with avascular necrosis of the left hip. Laboratory

More information

Meeting VAKB 8 februari 2011 Nancy Boeckx, MD, PhD

Meeting VAKB 8 februari 2011 Nancy Boeckx, MD, PhD Meeting VAKB 8 februari 2011 Nancy Boeckx, MD, PhD What is it? clonal expansion of myeloid precursor cells with reduced capacity to differentiate as opposed to ALL/CLL, it is limited to the myeloid cell

More information

JPEMS Nantes, Basic Immunology Introduction to the immune system Definitions Structure and General Organization

JPEMS Nantes, Basic Immunology Introduction to the immune system Definitions Structure and General Organization JPEMS Nantes, 2014- Basic Immunology Introduction to the immune system Definitions Structure and General Organization Teacher: Pr. Régis Josien, Laboratoire Immunologie and INSERM U1064, CHU Nantes Regis.Josien@univ-nantes.fr

More information

Hematopathology Case Study

Hematopathology Case Study www.medfusionservices.com Hematopathology Case Study CV3515-14 JUNE Clinical Presentation: Clinical Information: A 42 year old male with history of chronic myelogenous leukemia (CML) presents with an elevated

More information

Innate Immunity: (I) Molecules & (II) Cells. Part II: Cells (aka the Sentinels)

Innate Immunity: (I) Molecules & (II) Cells. Part II: Cells (aka the Sentinels) Innate Immunity: (I) Molecules & (II) Cells Stephanie Eisenbarth, M.D., Ph.D. FOCIS Advanced Course 2/19/18 Department of Laboratory Medicine Yale School of Medicine Department of Immunobiology Yale School

More information

Bone Marrow Stroma in Myelodysplastic Syndromes

Bone Marrow Stroma in Myelodysplastic Syndromes Bone Marrow Stroma in Myelodysplastic Syndromes Universidad de Salamanca Prof Mª M Consuelo del Cañizo Hematology Dept. University Hospital, Salamanca SPAIN Bone marrow stroma in MDS Introduction Mesenchymal

More information

Mixed Phenotype Acute Leukemias

Mixed Phenotype Acute Leukemias Mixed Phenotype Acute Leukemias CHEN GAO; AMY M. SANDS; JIANLAN SUN NORTH AMERICAN JOURNAL OF MEDICINE AND SCIENCE APR 2012 VOL 5 NO.2 INTRODUCTION Most cases of acute leukemia can be classified based

More information

Immunopathology. 2-Patterned hemodynamic responses, cell surface associated and soluble mediator systems (e.g., complement and coagulation systems).

Immunopathology. 2-Patterned hemodynamic responses, cell surface associated and soluble mediator systems (e.g., complement and coagulation systems). Immunopathology The chief role of the immune system is to protect the host from invasion by foreign agents. Immune responses can be elicited by a wide range of agents including toxins, drugs, chemicals,

More information

How plasma cells develop. Deutsches Rheuma Forschungs Zentrum, Berlin Institut der Leibniz Gemeinschaft

How plasma cells develop. Deutsches Rheuma Forschungs Zentrum, Berlin Institut der Leibniz Gemeinschaft How plasma cells develop Deutsches Rheuma Forschungs Zentrum, Berlin Institut der Leibniz Gemeinschaft 1 Plasma cells develop from activated B cells Toll Like Receptor B Cell Receptor B cell B cell microbia

More information

Effector T Cells and

Effector T Cells and 1 Effector T Cells and Cytokines Andrew Lichtman, MD PhD Brigham and Women's Hospital Harvard Medical School 2 Lecture outline Cytokines Subsets of CD4+ T cells: definitions, functions, development New

More information

SOPHOMORE DENTAL/OPTOMETRY MICROBIOLOGY SECTION: IMMUNOLOGY CELLS AND ORGANS OF THE IMMUNE SYSTEM

SOPHOMORE DENTAL/OPTOMETRY MICROBIOLOGY SECTION: IMMUNOLOGY CELLS AND ORGANS OF THE IMMUNE SYSTEM SOPHOMORE DENTAL/OPTOMETRY MICROBIOLOGY SECTION: IMMUNOLOGY CELLS AND ORGANS OF THE IMMUNE SYSTEM Lecturer: Dr. John F Kearney 934-6557 jfk@uab.edu Objectives: To learn: 1) the basic cell types that make

More information

Hematology MUHAMMAD M. KHURRAM* SAGHIR A. JAFRI* ABDUL MANNAN** AFTAB NADEEM*** ASIF JAMAL*

Hematology MUHAMMAD M. KHURRAM* SAGHIR A. JAFRI* ABDUL MANNAN** AFTAB NADEEM*** ASIF JAMAL* Hematology FREQUENCY OF ABERRANT EXPRESSION OF CD MARKERS IN CASES OF ACUTE LEUKEMIA MUHAMMAD M. KHURRAM* SAGHIR A. JAFRI* ABDUL MANNAN** AFTAB NADEEM*** ASIF JAMAL* SUMMARY: In the present study 100 patients

More information

Done By : WESSEN ADNAN BUTHAINAH AL-MASAEED

Done By : WESSEN ADNAN BUTHAINAH AL-MASAEED Done By : WESSEN ADNAN BUTHAINAH AL-MASAEED Acute Myeloid Leukemia Firstly we ll start with this introduction then enter the title of the lecture, so be ready and let s begin by the name of Allah : We

More information

Tim R. Randolph. PhD, MT(ASCP) Chair and Associate Professor Department of Biomedical Laboratory Science Saint Louis University

Tim R. Randolph. PhD, MT(ASCP) Chair and Associate Professor Department of Biomedical Laboratory Science Saint Louis University Tim R. Randolph. PhD, MT(ASCP) Chair and Associate Professor Department of Biomedical Laboratory Science Saint Louis University Anemias Over 30 types Myeloproliferative Neoplasm Polycythemia Leukemia AML:M6

More information

Acute Myeloid Leukemia with Recurrent Cytogenetic Abnormalities

Acute Myeloid Leukemia with Recurrent Cytogenetic Abnormalities Acute Myeloid Leukemia with Recurrent Cytogenetic Abnormalities Acute Myeloid Leukemia with recurrent cytogenetic Abnormalities -t(8;21)(q22;q22)(aml/eto) -inv(16) or t(16;16) -t(15;17) -11q23 Acute Myeloid

More information

A Multifaceted Immunomonitoring to Identify Predictive Biomarkers for the Clinical Outcome of Immunotherapy-Treated Melanoma Patients

A Multifaceted Immunomonitoring to Identify Predictive Biomarkers for the Clinical Outcome of Immunotherapy-Treated Melanoma Patients A Multifaceted Immunomonitoring to Identify Predictive Biomarkers for the Clinical Outcome of Immunotherapy-Treated Melanoma Patients Immunotherapy Biomarkers: Overcoming the Barriers NIH, Bethesda, April

More information

Blood Cells Med Terms Quiz

Blood Cells Med Terms Quiz Blood Cells Med Terms Quiz Question Prompt: 1 Mononuclear white blood cells (agranulocyte) formed in lymph tissue, also a phagocyte and a precursor of macrophages are leukocytes. True False Question Prompt:

More information

Alessandra Franco, M.D., Ph.D. UCSD School of Medicine Department of Pediatrics Division of Allergy, Immunology and Rheumatology

Alessandra Franco, M.D., Ph.D. UCSD School of Medicine Department of Pediatrics Division of Allergy, Immunology and Rheumatology Natural regulatory T cells recognize the heavy constant region (Fc) of immunoglobulins: a novel mechanism for IVIG immunotherapy in Pediatric Immune-mediated diseases Alessandra Franco, M.D., Ph.D. UCSD

More information

Development of B and T lymphocytes

Development of B and T lymphocytes Development of B and T lymphocytes What will we discuss today? B-cell development T-cell development B- cell development overview Stem cell In periphery Pro-B cell Pre-B cell Immature B cell Mature B cell

More information

Production of the Formed Elements *

Production of the Formed Elements * OpenStax-CNX module: m46691 1 Production of the Formed Elements * OpenStax This work is produced by OpenStax-CNX and licensed under the Creative Commons Attribution License 3.0 By the end of this section,

More information

x Lymphocyte count /µl CD8+ count/µl 800 Calculated

x Lymphocyte count /µl CD8+ count/µl 800 Calculated % Lymphocyte in CBC A. 50 40 30 20 10 Lymphocyte count /µl B. x10 3 2.5 1.5 C. 50 D. 1000 % CD3+CD8+ Cells 40 30 20 Calculated CD8+ count/µl 800 600 400 200 10 0 #61 #63 #64 #65 #68 #71 #72 #75 Figure

More information

Abnormalities of the Immune System and Inflammation in MDS Pathogenesis. Shahram Kordasti MSc(Med Imm), MD, PhD Department of Haematological Medicine

Abnormalities of the Immune System and Inflammation in MDS Pathogenesis. Shahram Kordasti MSc(Med Imm), MD, PhD Department of Haematological Medicine Abnormalities of the Immune System and Inflammation in MDS Pathogenesis Shahram Kordasti MSc(Med Imm), MD, PhD Department of Haematological Medicine What is the role of immune system in MDS? Oligoclonal

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION doi:10.1038/nature10134 Supplementary Figure 1. Anti-inflammatory activity of sfc. a, Autoantibody immune complexes crosslink activating Fc receptors, promoting activation of macrophages, and WWW.NATURE.COM/NATURE

More information