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1 Supplemental Figure Legends Supplemental Figure 1. SemaB / mice have normal immune cell populations. Cells were prepared from the spleens of WT and SemaB / mice, stained with various antibodies and then analyzed by flow cytometry. Supplemental Figure 2. SemaB / T cells, B cells and DCs have normal proliferation, cytokine production and cellular interactions. A, B, Normal proliferative responses in SemaB / B cells and T cells. Splenic B cells from WT (open circles) and SemaB / (closed circles) mice were cultured with various concentrations of anti-μ or anti-cd for 36 h (A). Splenic CD + T cells from WT (open circles) and SemaB / (closed circles) mice were cultured with immobilized anti-cd3 with or without anti-cd28 for 8 h (B). [ 3 H] thymidine was added for the last 1 h. C, Th1/Th2 skewing was not impaired in SemaB / T cells. WT (open circles) or SemaB / (closed circles) naïve CD62L + CD + T cells were cultured under Th1- (left panel) or Th2- (right panel) skewing conditions for days. Then, CD + T cells were restimulated with immobilized anti-cd3 for 2 h. The cytokine concentrations in the culture supernatants were measured by ELISA. D, Normal cytokine production of SemaB / DCs. Splenic DCs (left panel) or BMDCs (right panel) from WT (open circles) or
2 SemaB / (closed circles) mice were stimulated with anti-cd for 8 h. The concentration of IL-12p in the culture supernatants was measured by ELISA. E, SemaB was not involved in normal MLRs between T cells and DCs. CD + T cells from BALB/c mice were cultured with irradiated allogeneic C7BL/6 WT (open circles) or SemaB / (closed circles) DCs for 6 h (upper panel). CD + T cells from C7BL/6 WT (open circles) or SemaB / (closed circles) mice were cultured with irradiated allogeneic BALB/c DCs for 6 h (lower panel). [ 3 H] thymidine was added for the last 1 h. Supplemental Figure 3. does not affect B cells, T cells and DCs in terms of proliferation, cytokine production and antibody production. B cell proliferation (A), B cell antibody production (B), T cell proliferation (C), splenic DC (left panel) and BMDC (right panel) cytokine production (D) and MLRs between CD + T cells and allogeneic DCs (E) were analyzed in the presence of (closed bars or circles) or (open bars or circles). F, did not affect DC-mediated Th1/Th2 differentiation. Naïve CD62L + CD + T cells derived from OVA-TCR Tg mice and BMDCs were cultured with OVA peptide for days in the presence of (closed circles) or (open circles). Then, CD + T cells were isolated and stimulated with
3 immobilized anti-cd3. The concentrations of IFN-γ (left panel) and IL- (right panel) in the culture supernatants were measured by ELISA. Supplemental Figure. affects basophils in a dose-dependent manner. BM-derived basophils were stimulated with IL-3 in the presence of various concentrations of (closed and semi-closed bars) or (open and semi-open bars) and then examined for IL- production. Supplemental Figure. SemaB / basophils have normal IL- production. WT (open bars) or SemaB / (closed bars) BM-derived basophils were cultured with IgE anti-dnp plus various concentrations of DNP-HSA for 16 h. The cytokine concentrations in the culture supernatants were measured by ELISA.
4 / / CD CD CD Foxp B CD CD11c DX 1 1 CD CD Supplemental Figure 1
5 A D Splenic DC BMDC onses p.m) B cell respo ( 1 3 c.p B T ce ell responses ( 1 3 c.p.m) C IFN-γ (ng/m ml) Anti-μ (μg/ml) Th1 condition onses p.m) B cell resp ( 1 3 c.p T ce ell responses ( 1 3 c.p.m) IL- (ng/m ml) Anti-CD (μg/ml) + Anti-CD Th2 condition E IL-12p (ng/ml) T ce ell responses ( 1 3 c.p.m) nses.m) T cell respo ( 1 3 c.p Anti-CD (μg/ml) IL-12p (ng/ml) DCs ( 1 3 cells/well) 1 Anti-CD (μg/ml) DCs (C7BL/6) T cells (BALB/c) DCs T cells (BALB/c) (C7BL/6) DCs ( 1 3 cells/well) Supplemental Figure 2
6 A C B cell resp ponses ( 1 3 c.p.m) responses 3 c.p.m) T cell ( 1 E T cell response es ( 1 3 c.p.m ) Anti-μ B IgM (μg g/ml) D /ml) IgG1 (ng IL-12p p (ng/ml) ml) IgE (ng/ IL- IL- IL- 6 2 Splenic DC IL-12p p (ng/ml) 9 6 BMDC Anti-CD3 AtiCD Anti-CD (μg/ml) AtiCD Anti-CD (μg/ml) DCs ( 1 3 cells/well) F IFN-g (ng/m ml) IL- (pg/m ml) Supplemental Figure 3
7 9 *** /ml) IL- (pg/ 6 3 N.D. + IL-3 (2 μg/ml) (1 μg/ml) (2 μg/ml) (1 μg/ml) Supplemental Figure
8 6 9 IL- (ng/ml) 2 IL-6 (ng/ml) 6 3 N.D. 1 1 N.D. 1 1 DNP-HSA (ng/ml) DNP-HSA (ng/ml) Supplemental Figure
Supplementary Figure 1. Characterization of basophils after reconstitution of SCID mice
Supplementary figure legends Supplementary Figure 1. Characterization of after reconstitution of SCID mice with CD4 + CD62L + T cells. (A-C) SCID mice (n = 6 / group) were reconstituted with 2 x 1 6 CD4
Supplementary Figure 1 Protease allergens induce IgE and IgG1 production. (a-c)
1 Supplementary Figure 1 Protease allergens induce IgE and IgG1 production. (a-c) Serum IgG1 (a), IgM (b) and IgG2 (c) concentrations in response to papain immediately before primary immunization (day
SUPPLEMENTARY INFORMATION
doi:1.138/nature1554 a TNF-α + in CD4 + cells [%] 1 GF SPF 6 b IL-1 + in CD4 + cells [%] 5 4 3 2 1 Supplementary Figure 1. Effect of microbiota on cytokine profiles of T cells in GALT. Frequencies of TNF-α
Supplementary Figure 1. IL-12 serum levels and frequency of subsets in FL patients. (A) IL-12
1 Supplementary Data Figure legends Supplementary Figure 1. IL-12 serum levels and frequency of subsets in FL patients. (A) IL-12 serum levels measured by multiplex ELISA (Luminex) in FL patients before
Supplemental Materials for. Effects of sphingosine-1-phosphate receptor 1 phosphorylation in response to. FTY720 during neuroinflammation
Supplemental Materials for Effects of sphingosine-1-phosphate receptor 1 phosphorylation in response to FTY7 during neuroinflammation This file includes: Supplemental Table 1. EAE clinical parameters of
Supplementary Fig. 1 p38 MAPK negatively regulates DC differentiation. (a) Western blot analysis of p38 isoform expression in BM cells, immature DCs
Supplementary Fig. 1 p38 MAPK negatively regulates DC differentiation. (a) Western blot analysis of p38 isoform expression in BM cells, immature DCs (idcs) and mature DCs (mdcs). A myeloma cell line expressing
Supplemental Figure 1
Supplemental Figure 1 1a 1c PD-1 MFI fold change 6 5 4 3 2 1 IL-1α IL-2 IL-4 IL-6 IL-1 IL-12 IL-13 IL-15 IL-17 IL-18 IL-21 IL-23 IFN-α Mut Human PD-1 promoter SBE-D 5 -GTCTG- -1.2kb SBE-P -CAGAC- -1.kb
Hua Tang, Weiping Cao, Sudhir Pai Kasturi, Rajesh Ravindran, Helder I Nakaya, Kousik
SUPPLEMENTARY FIGURES 1-19 T H 2 response to cysteine-proteases requires dendritic cell-basophil cooperation via ROS mediated signaling Hua Tang, Weiping Cao, Sudhir Pai Kasturi, Rajesh Ravindran, Helder
SUPPLEMENTARY INFORMATION
Supplemental Figure 1. Furin is efficiently deleted in CD4 + and CD8 + T cells. a, Western blot for furin and actin proteins in CD4cre-fur f/f and fur f/f Th1 cells. Wild-type and furin-deficient CD4 +
Nature Medicine: doi: /nm.3922
Title: Glucocorticoid-induced tumor necrosis factor receptor-related protein co-stimulation facilitates tumor regression by inducing IL-9-producing helper T cells Authors: Il-Kyu Kim, Byung-Seok Kim, Choong-Hyun
The encephalitogenicity of TH17 cells is dependent on IL-1- and IL-23- induced production of the cytokine GM-CSF
CORRECTION NOTICE Nat.Immunol. 12, 568 575 (2011) The encephalitogenicity of TH17 cells is dependent on IL-1- and IL-23- induced production of the cytokine GM-CSF Mohamed El-Behi, Bogoljub Ciric, Hong
Supplementary Figure 1. Normal T lymphocyte populations in Dapk -/- mice. (a) Normal thymic development in Dapk -/- mice. Thymocytes from WT and Dapk
Supplementary Figure 1. Normal T lymphocyte populations in Dapk -/- mice. (a) Normal thymic development in Dapk -/- mice. Thymocytes from WT and Dapk -/- mice were stained for expression of CD4 and CD8.
Supplementary Figure Legends. group) and analyzed for Siglec-G expression utilizing a monoclonal antibody to Siglec-G (clone SH2.1).
Supplementary Figure Legends Supplemental Figure : Naïve T cells express Siglec-G. Splenocytes were isolated from WT B or Siglec-G -/- animals that have not been transplanted (n= per group) and analyzed
B6/COLODR/SPL/11C/83/LAP/#2.006 B6/COLODR/SPL/11C/86/LAP/#2.016 CD11C B6/COLODR/SPL/11C/80/LAP/#2.011 CD11C
CD3-specific antibody-induced immune tolerance and suppression of autoimmune encephalomyelitis involves TGF-β production through phagocytes digesting apoptotic T cells Sylvain Perruche 1,3, Pin Zhang 1,
Supplementary Figure S1. PTPN2 levels are not altered in proliferating CD8+ T cells. Lymph node (LN) CD8+ T cells from C57BL/6 mice were stained with
Supplementary Figure S1. PTPN2 levels are not altered in proliferating CD8+ T cells. Lymph node (LN) CD8+ T cells from C57BL/6 mice were stained with CFSE and stimulated with plate-bound α-cd3ε (10µg/ml)
NK cell flow cytometric assay In vivo DC viability and migration assay
NK cell flow cytometric assay 6 NK cells were purified, by negative selection with the NK Cell Isolation Kit (Miltenyi iotec), from spleen and lymph nodes of 6 RAG1KO mice, injected the day before with
Supplementary Figures
Supplementary Figures Supplementary Figure 1. NKT ligand-loaded tumour antigen-presenting B cell- and monocyte-based vaccine induces NKT, NK and CD8 T cell responses. (A) The cytokine profiles of liver
Supplemental Figure 1. Signature gene expression in in vitro differentiated Th0, Th1, Th2, Th17 and Treg cells. (A) Naïve CD4 + T cells were cultured
Supplemental Figure 1. Signature gene expression in in vitro differentiated Th0, Th1, Th2, Th17 and Treg cells. (A) Naïve CD4 + T cells were cultured under Th0, Th1, Th2, Th17, and Treg conditions. mrna
SUPPLEMENTARY INFORMATION
doi: 1.138/nature775 4 O.D. (595-655) 3 1 -ζ no antibody isotype ctrl Plated Soluble 1F6 397 7H11 Supplementary Figure 1 Soluble and plated anti- Abs induce -! signalling. B3Z cells stably expressing!
Blocking antibodies and peptides. Rat anti-mouse PD-1 (29F.1A12, rat IgG2a, k), PD-
Supplementary Methods Blocking antibodies and peptides. Rat anti-mouse PD-1 (29F.1A12, rat IgG2a, k), PD- L1 (10F.9G2, rat IgG2b, k), and PD-L2 (3.2, mouse IgG1) have been described (24). Anti-CTLA-4 (clone
Supplementary Figure 1. Efficient DC depletion in CD11c.DOG transgenic mice
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Supplementary Information:
Supplementary Information: Follicular regulatory T cells with Bcl6 expression suppress germinal center reactions by Yeonseok Chung, Shinya Tanaka, Fuliang Chu, Roza Nurieva, Gustavo J. Martinez, Seema
Supplementary Figure 1. mrna expression of chitinase and chitinase-like protein in splenic immune cells. Each splenic immune cell population was
Supplementary Figure 1. mrna expression of chitinase and chitinase-like protein in splenic immune cells. Each splenic immune cell population was sorted by FACS. Surface markers for sorting were CD11c +
Supplemental Figure 1. Protein L
Supplemental Figure 1 Protein L m19delta T m1928z T Suppl. Fig 1. Expression of CAR: B6-derived T cells were transduced with m19delta (left) and m1928z (right) to generate CAR T cells and transduction
ECM1 controls T H 2 cell egress from lymph nodes through re-expression of S1P 1
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Supplementary Figure 1. ETBF activate Stat3 in B6 and Min mice colons
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Supplemental Table I.
Supplemental Table I Male / Mean ± SEM n Mean ± SEM n Body weight, g 29.2±0.4 17 29.7±0.5 17 Total cholesterol, mg/dl 534.0±30.8 17 561.6±26.1 17 HDL-cholesterol, mg/dl 9.6±0.8 17 10.1±0.7 17 Triglycerides,
Canberra, Australia). CD11c-DTR-OVA-GFP (B6.CD11c-OVA), B6.luc + and. Cancer Research Center, Germany). B6 or BALB/c.FoxP3-DTR-GFP mice were
Supplemental Materials and Methods Mice Female C57BL/6 (B6, I-E null, H-2 b ), BALB/c (H-2 d ) + ), FVB/N (H-2 q, I-E null, CD45.1 + ), and B6D2F1 (H-2 b/d ) mice were purchased from the Animal Resources
MATERIALS AND METHODS. Neutralizing antibodies specific to mouse Dll1, Dll4, J1 and J2 were prepared as described. 1,2 All
MATERIALS AND METHODS Antibodies (Abs), flow cytometry analysis and cell lines Neutralizing antibodies specific to mouse Dll1, Dll4, J1 and J2 were prepared as described. 1,2 All other antibodies used
% of live splenocytes. STAT5 deletion. (open shapes) % ROSA + % floxed
Supp. Figure 1. a 14 1 1 8 6 spleen cells (x1 6 ) 16 % of live splenocytes 5 4 3 1 % of live splenocytes 8 6 4 b 1 1 c % of CD11c + splenocytes (closed shapes) 8 6 4 8 6 4 % ROSA + (open shapes) % floxed
SUPPLEMENTARY FIGURE 1
SUPPLEMENTARY FIGURE 1 A LN Cell count (1 ) 1 3 1 CD+ 1 1 CDL lo CD hi 1 CD+FoxP3+ 1 1 1 7 3 3 3 % of cells 9 7 7 % of cells CD+ 3 1 % of cells CDL lo CD hi 1 1 % of CD+ cells CD+FoxP3+ 3 1 % of CD+ T
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Cell isolation. Spleen and lymph nodes (axillary, inguinal) were removed from mice
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Supplemental Materials
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and follicular helper T cells is Egr2-dependent. (a) Diagrammatic representation of the
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Supporting Information Table of Contents
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Tbk1-TKO! DN cells (%)! 15! 10!
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doi: 1.138/nature89 IFN- (ng ml ) 5 4 3 1 Splenocytes NS IFN- (ng ml ) 6 4 Lymph node cells NS Nfkbiz / Nfkbiz / Nfkbiz / Nfkbiz / IL- (ng ml ) 3 1 Splenocytes IL- (ng ml ) 1 8 6 4 *** ** Lymph node cells