Product Datasheet. HLA ABC Antibody (W6/32) NB Unit Size: 0.25 mg. Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 22

Size: px
Start display at page:

Download "Product Datasheet. HLA ABC Antibody (W6/32) NB Unit Size: 0.25 mg. Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 22"

Transcription

1 Product Datasheet HLA ABC Antibody (W6/32) NB Unit Size: 0.25 mg Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 22 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 5/30/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB HLA ABC Antibody (W6/32) Product Information Unit Size Concentration Storage Clonality 0.25 mg 1 mg/ml Store at -20C. Avoid freeze-thaw cycles. Monoclonal Clone W6/32 Preservative Isotype Purity Buffer No Preservative IgG2a Protein G purified Phosphate buffered saline Page 1 of 6 v.20.1 Updated 5/30/2018 Product Description Host Mouse Gene ID 3105 Gene Symbol Species Specificity/Sensitivity Immunogen Product Application Details Applications HLA-A Human, Baboon, Bovine, Primate NB recognizes an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (6). MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes. Purified human tonsil lymphocyte membranes. ELISA, Flow Cytometry, Functional, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation Recommended Dilutions Flow Cytometry 1:50-1:100, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, Functional Application Notes Images Antibody does not work in Immunohistochemistry-Paraffin. Flow Cytometry: HLA ABC Antibody (W6/32) [NB ] - Analysis using the FITC conjugate of NB Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control FITC (NBP ) (blue histogram) or Anti-Human HLA-ABC FITC (purple histogram). Cells in the lymphocyte gate were used f

3 ABC. Page 2 of 6 v.20.1 Updated 5/30/2018 ABC:Alexa Fluor 488. ABC:Alexa Fluor 647. ABC:FITC.

4 of human peripheral blood monocytes with Mouse anti Human HLA ABC:Biotin. Page 3 of 6 v.20.1 Updated 5/30/2018 Flow Cytometry: HLA ABC Antibody (W6/32) [NB ] - Human lymphoblast. Image provided via verified customer review. of peripheral blood lymphocytes with anti-hla-abc: Low Endotoxin. Flow Cytometry: HLA ABC Antibody (W6/32) [NB ] - Analysis using the PE/Cy5 conjugate of NB Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control PE-Cy5 (NBP ) (blue histogram) or Anti-Human HLA-ABC PE-Cy5 (purple histogram). Cells in the lymphocyte gate were

5 Publications Goldman-Wohl D, Ariel I et al. A study of human leukocyte antigen G expression in hydatidiform moles Am J Obstet Gynecol Jun :00AM (IHC-Fr) This publication used the FITC conjugated form of this antibody (Cat# NBP ). Koene G, Arts-Hilkes et al. High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss Hum Immunol Jun :00AM This publication used the FITC conjugated form of this antibody (Cat# NBP ). Koene G, Arts-Hilkes et al. High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss Hum Immunol 2004 [PMID: ] This citation used the FITC version of this antibody. Goldman-Wohl D, Ariel I et al. A study of human leukocyte antigen G expression in hydatidiform moles Am J Obstet Gynecol 2001 [PMID: ] (IHC-Fr) This citation used the FITC version of this antibody. Jones, DC et al HLA Class I Allelic Sequence and Conformation Regulate Leukocyte Ig-Like Receptor Binding. J Immunol 186: [PMID: ] Zuo, J et al. The Epstein-Barr virus-encoded BILF1 protein modulates immune recognition of endogenously processed antigen by targeting major histocompatibility complex class I molecules trafficking on both the exocytic and endocytic pathways. J Virol 85: [PMID: ] Fujita, Y et al. Bone marrow transplantation restores epidermal basement membrane protein expression and rescues epidermolysis bullosa model mice. Proc Natl Acad Sci U S A 107: [PMID: ] Narita, M et al. WT1 peptide vaccination in combination with imatinib therapy for a patient with CML in the chronic phase. Int J Med Sci 7: [PMID: ] Vitadello, M et al. Myofiber stress-response in myositis: parallel investigations on patients and experimental animal models of muscle regeneration and systemic inflammation. Arthritis Res Ther 12: R [PMID: ] Hinrichs, J et al. The nature of peptides presented by an HLA class I low expression allele. Haematologica 95: [PMID: ] Spentzou, A et al. Viral inhibition assay: a CD8 T cell neutralization assay for use in clinical trials of HIV-1 vaccine candidates. J Infect Dis 201: [PMID: ] Grotzke, JE et al. The Mycobacterium tuberculosis phagosome is a HLA-I processing competent organelle. PLoS Pathog 5: e [PMID: ] More publications at Page 4 of 6 v.20.1 Updated 5/30/2018

6 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF007 NB720-B NBP Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] (Preadsorbed) Mouse IgG2a Isotype Control (M2A) NB UV HLA ABC Antibody (W6/32) [DyLight 350] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product:

7

Product Datasheet. CD161/NK1.1 Antibody (PK136) NB Unit Size: 0.5 mg. Store at 4C. Do not freeze. Publications: 2

Product Datasheet. CD161/NK1.1 Antibody (PK136) NB Unit Size: 0.5 mg. Store at 4C. Do not freeze. Publications: 2 Product Datasheet CD161/NK1.1 Antibody (PK136) NB100-77528 Unit Size: 0.5 mg Store at 4C. Do not freeze. Publications: 2 Protocols, Publications, Related Products, Reviews, Research Tools and Images at:

More information

Product Datasheet. Endothelin-1 Antibody (TR.ET.48.5) NB Unit Size: 100 ul. Store at -20C. Avoid freeze-thaw cycles.

Product Datasheet. Endothelin-1 Antibody (TR.ET.48.5) NB Unit Size: 100 ul. Store at -20C. Avoid freeze-thaw cycles. Product Datasheet Endothelin-1 Antibody (TR.ET.48.5) NB300-526 Unit Size: 100 ul Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 2 Protocols, Publications, Related Products, Reviews,

More information

Product Datasheet. EMMPRIN/CD147 Antibody (MEM-M6/1) NB Unit Size: 0.1 mg. Store at 4C. Do not freeze. Publications: 2

Product Datasheet. EMMPRIN/CD147 Antibody (MEM-M6/1) NB Unit Size: 0.1 mg. Store at 4C. Do not freeze. Publications: 2 Product Datasheet EMMPRIN/CD147 Antibody (MEM-M6/1) NB500-430 Unit Size: 0.1 mg Store at 4C. Do not freeze. Publications: 2 Protocols, Publications, Related Products, Reviews, Research Tools and Images

More information

Product Datasheet. Ly-6G6C Antibody (NIMP-R14) NB Unit Size: 0.05 mg. Store at 4C. Do not freeze. Publications: 23

Product Datasheet. Ly-6G6C Antibody (NIMP-R14) NB Unit Size: 0.05 mg. Store at 4C. Do not freeze. Publications: 23 Product Datasheet Ly-6G6C Antibody (NIMP-R14) NB600-1387 Unit Size: 0.05 mg Store at 4C. Do not freeze. Publications: 23 Protocols, Publications, Related Products, Reviews, Research Tools and Images at:

More information

Product Datasheet. PMP70 Antibody (CL2524) NBP Unit Size: 0.1 ml

Product Datasheet. PMP70 Antibody (CL2524) NBP Unit Size: 0.1 ml Product Datasheet PMP70 Antibody (CL2524) NBP2-36770 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 1 Protocols, Publications, Related

More information

Product Datasheet. CD133 Antibody NB Unit Size: 0.1 mg

Product Datasheet. CD133 Antibody NB Unit Size: 0.1 mg Product Datasheet CD133 Antibody NB120-16518 Unit Size: 0.1 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 8 Protocols, Publications, Related Products,

More information

Product Datasheet. SERCA2 ATPase Antibody (IID8) NB Unit Size: 100uL. Store at -20C. Avoid freeze-thaw cycles.

Product Datasheet. SERCA2 ATPase Antibody (IID8) NB Unit Size: 100uL. Store at -20C. Avoid freeze-thaw cycles. Product Datasheet SERCA2 ATPase Antibody (IID8) NB300-529 Unit Size: 100uL Store at -20C. Avoid freeze-thaw cycles. Publications: 5 Protocols, Publications, Related Products, Reviews, Research Tools and

More information

Product Datasheet. IGF-I R Antibody (3G5C1) NB Unit Size: 0.1 ml

Product Datasheet. IGF-I R Antibody (3G5C1) NB Unit Size: 0.1 ml Product Datasheet IGF-I R Antibody (3G5C1) NB110-87052 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 2 Publications: 8 Protocols, Publications,

More information

Product Datasheet. DARC Antibody NB Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles. Publications: 5

Product Datasheet. DARC Antibody NB Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles. Publications: 5 Product Datasheet DARC Antibody NB100-2421 Unit Size: 0.1 mg Store at -20C. Avoid freeze-thaw cycles. Publications: 5 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/nb100-2421

More information

Product Datasheet. DC-LAMP Antibody (104G4) DDX0190P-100. Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles.

Product Datasheet. DC-LAMP Antibody (104G4) DDX0190P-100. Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles. Product Datasheet DC-LAMP Antibody (104G4) DDX0190P-100 Unit Size: 0.1 mg Store at -20C. Avoid freeze-thaw cycles. Publications: 18 Protocols, Publications, Related Products, Reviews, Research Tools and

More information

Product Datasheet. HLA-DR Antibody (L243) NB SS. Unit Size: ml

Product Datasheet. HLA-DR Antibody (L243) NB SS. Unit Size: ml Product Datasheet HLA-DR Antibody (L243) NB100-77855SS Unit Size: 0.025 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 26 Protocols, Publications,

More information

Product Datasheet. ERCC1 Antibody (8F1) NB Unit Size: 0.1 ml

Product Datasheet. ERCC1 Antibody (8F1) NB Unit Size: 0.1 ml Product Datasheet ERCC1 Antibody (8F1) NB500-704 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 1 Publications: 15 Protocols, Publications,

More information

Product Datasheet. Vanilloid R1/TRPV1 Antibody NB Unit Size: 0.05 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Product Datasheet. Vanilloid R1/TRPV1 Antibody NB Unit Size: 0.05 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Product Datasheet Vanilloid R1/TRPV1 Antibody NB100-1617 Unit Size: 0.05 ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Publications: 15 Protocols, Publications, Related Products, Reviews,

More information

Product Datasheet. DLL4 Antibody NB Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles. Publications: 14

Product Datasheet. DLL4 Antibody NB Unit Size: 0.1 mg. Store at -20C. Avoid freeze-thaw cycles. Publications: 14 Product Datasheet DLL4 Antibody NB600-892 Unit Size: 0.1 mg Store at -20C. Avoid freeze-thaw cycles. Publications: 14 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/nb600-892

More information

Product Datasheet. MMR/CD206/Mannose Receptor Antibody (5C11) H M02. Unit Size: 0.1 mg

Product Datasheet. MMR/CD206/Mannose Receptor Antibody (5C11) H M02. Unit Size: 0.1 mg Product Datasheet MMR/CD206/Mannose Receptor Antibody (5C11) H00004360-M02 Unit Size: 0.1 mg Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Publications: 14 Protocols, Publications, Related

More information

Product Datasheet. CD68/SR-D1 Antibody (KP1) NB Unit Size: 0.5 ml. Store at 4C. Do not freeze. Publications: 34

Product Datasheet. CD68/SR-D1 Antibody (KP1) NB Unit Size: 0.5 ml. Store at 4C. Do not freeze. Publications: 34 Product Datasheet CD68/SR-D1 Antibody (KP1) NB100-683 Unit Size: 0.5 ml Store at 4C. Do not freeze. Publications: 34 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/nb100-683

More information

Product Datasheet. inos Antibody NB Unit Size: 200uL. Store at -20C. Avoid freeze-thaw cycles. Reviews: 2 Publications: 28

Product Datasheet. inos Antibody NB Unit Size: 200uL. Store at -20C. Avoid freeze-thaw cycles. Reviews: 2 Publications: 28 Product Datasheet inos Antibody NB300-605 Unit Size: 200uL Store at -20C. Avoid freeze-thaw cycles. Reviews: 2 Publications: 28 Protocols, Publications, Related Products, Reviews, Research Tools and Images

More information

Product Datasheet. KAP1 Antibody NB Unit Size: 100 ul. Store at 4C. Do not freeze. Publications: 16

Product Datasheet. KAP1 Antibody NB Unit Size: 100 ul. Store at 4C. Do not freeze. Publications: 16 Product Datasheet KAP1 Antibody NB500-158 Unit Size: 100 ul Store at 4C. Do not freeze. Publications: 16 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/nb500-158

More information

Product Datasheet. p14arf/cdkn2a Antibody NB Unit Size: 0.1 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Product Datasheet. p14arf/cdkn2a Antibody NB Unit Size: 0.1 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Product Datasheet p14arf/cdkn2a Antibody NB200-111 Unit Size: 0.1 ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Reviews: 2 Publications: 16 Protocols, Publications, Related Products,

More information

Product Datasheet. IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) NB Unit Size: 0.1 mg

Product Datasheet. IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) NB Unit Size: 0.1 mg Product Datasheet IkB-alpha [p Ser32, p Ser36] Antibody (39A1413) NB100-56724 Unit Size: 0.1 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 11 Protocols,

More information

Product Datasheet. Caspase-8 Antibody - (active/cleaved) NB Unit Size: 0.05 ml. Store at -20C. Avoid freeze-thaw cycles.

Product Datasheet. Caspase-8 Antibody - (active/cleaved) NB Unit Size: 0.05 ml. Store at -20C. Avoid freeze-thaw cycles. Product Datasheet Caspase-8 Antibody - (active/cleaved) NB100-56116 Unit Size: 0.05 ml Store at -20C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 38 Protocols, Publications, Related Products, Reviews,

More information

Product Datasheet. Caspase-3 Antibody - (active/cleaved) NB Unit Size: 0.05 ml. Store at -20C. Avoid freeze-thaw cycles.

Product Datasheet. Caspase-3 Antibody - (active/cleaved) NB Unit Size: 0.05 ml. Store at -20C. Avoid freeze-thaw cycles. Product Datasheet Caspase-3 Antibody - (active/cleaved) NB100-56113 Unit Size: 0.05 ml Store at -20C. Avoid freeze-thaw cycles. Publications: 16 Protocols, Publications, Related Products, Reviews, Research

More information

Product Datasheet. ZEB1 Antibody NBP Unit Size: 0.1 ml. Store at 4C. Do not freeze. Reviews: 6 Publications: 32

Product Datasheet. ZEB1 Antibody NBP Unit Size: 0.1 ml. Store at 4C. Do not freeze. Reviews: 6 Publications: 32 Product Datasheet ZEB1 Antibody NBP1-05987 Unit Size: 0.1 ml Store at 4C. Do not freeze. Reviews: 6 Publications: 32 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/nbp1-05987

More information

Product Datasheet. Caspase-3 Antibody (31A1067) - (Pro and Active) NB Unit Size: 0.1 mg

Product Datasheet. Caspase-3 Antibody (31A1067) - (Pro and Active) NB Unit Size: 0.1 mg Product Datasheet Caspase-3 Antibody (31A1067) - (Pro and Active) NB100-56708 Unit Size: 0.1 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 1 Publications:

More information

Product Datasheet. beta Amyloid Antibody (MOAB-2) NBP Unit Size: 0.1 ml

Product Datasheet. beta Amyloid Antibody (MOAB-2) NBP Unit Size: 0.1 ml Product Datasheet beta Amyloid Antibody (MOAB-2) NBP2-13075 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 11 Protocols, Publications,

More information

Product Datasheet. STING/TMEM173 Antibody NBP Unit Size: 0.1 mg

Product Datasheet. STING/TMEM173 Antibody NBP Unit Size: 0.1 mg Product Datasheet STING/TMEM173 Antibody NBP2-24683 Unit Size: 0.1 mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Publications: 12 Protocols, Publications, Related

More information

Product Datasheet. CD4 Antibody NBP Unit Size: 0.1 ml. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Datasheet. CD4 Antibody NBP Unit Size: 0.1 ml. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Product Datasheet CD4 Antibody NBP1-19371 Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 7 Publications: 13 Protocols, Publications, Related

More information

Product Datasheet. Glut4 Antibody NBP Unit Size: 0.1 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Product Datasheet. Glut4 Antibody NBP Unit Size: 0.1 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Product Datasheet Glut4 Antibody NBP1-49533 Unit Size: 0.1 ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Reviews: 3 Publications: 8 Protocols, Publications, Related Products, Reviews,

More information

Product Datasheet. Annexin V Apoptosis Kit [FITC] NBP Tests. Unit Size: 100 Tests. Store at 4C. Do not freeze.

Product Datasheet. Annexin V Apoptosis Kit [FITC] NBP Tests. Unit Size: 100 Tests. Store at 4C. Do not freeze. Product Datasheet Annexin V Apoptosis Kit [FITC] NBP2-29373-100Tests Unit Size: 100 Tests Store at 4C. Do not freeze. Publications: 20 Protocols, Publications, Related Products, Reviews, Research Tools

More information

Product Datasheet. TLR4 Inhibitor Peptide Set NBP Unit Size: 2 Vials

Product Datasheet. TLR4 Inhibitor Peptide Set NBP Unit Size: 2 Vials Product Datasheet TLR4 Inhibitor Peptide Set NBP2-26244 Unit Size: 2 Vials Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 1 Publications: 32 Protocols,

More information

Anti-DC-SIGN/CD209 murine monoclonal antibodies

Anti-DC-SIGN/CD209 murine monoclonal antibodies Anti-DC-SIGN/CD209 murine monoclonal antibodies DC-SIGN (DC Specific, ICAM-3 Grabbing, Nonintegrin) / CD209 and L-SIGN (liver/lymph node-specific ICAM-3-grabbing nonintegrin CD299/ DC-SIGNR (DC-SIGN-related

More information

Anti-PD-L1 antibody [28-8] ab205921

Anti-PD-L1 antibody [28-8] ab205921 Anti-PD-L1 antibody [28-8] ab205921 2 Abreviews 16 References 15 Images Overview Product name Anti-PD-L1 antibody [28-8] Description Tested applications Species reactivity Immunogen Rabbit monoclonal [28-8]

More information

Complement Antibodies and Proteins

Complement Antibodies and Proteins COMPLEMENT ANTIBODIES AND PROTEINS FROM CEDARLANE INTERNATIONAL VERSION www.cedarlanelabs.com For over 50 years, Cedarlane has provided complement related products to the life science research and diagnostic

More information

Anti-Lamin B1/LMNB1 Picoband Antibody

Anti-Lamin B1/LMNB1 Picoband Antibody Anti-Lamin B1/LMNB1 Picoband Antibody Catalog Number:PB9611 About LMNB1 Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins

More information

Helminth worm, Schistosomiasis Trypanosomes, sleeping sickness Pneumocystis carinii. Ringworm fungus HIV Influenza

Helminth worm, Schistosomiasis Trypanosomes, sleeping sickness Pneumocystis carinii. Ringworm fungus HIV Influenza Helminth worm, Schistosomiasis Trypanosomes, sleeping sickness Pneumocystis carinii Ringworm fungus HIV Influenza Candida Staph aureus Mycobacterium tuberculosis Listeria Salmonella Streptococcus Levels

More information

Principles of Adaptive Immunity

Principles of Adaptive Immunity Principles of Adaptive Immunity Chapter 3 Parham Hans de Haard 17 th of May 2010 Agenda Recognition molecules of adaptive immune system Features adaptive immune system Immunoglobulins and T-cell receptors

More information

Supplementary Data 1. Alanine substitutions and position variants of APNCYGNIPL. Applied in

Supplementary Data 1. Alanine substitutions and position variants of APNCYGNIPL. Applied in Supplementary Data 1. Alanine substitutions and position variants of APNCYGNIPL. Applied in Supplementary Fig. 2 Substitution Sequence Position variant Sequence original APNCYGNIPL original APNCYGNIPL

More information

MHC Tetramers and Monomers for Immuno-Oncology and Autoimmunity Drug Discovery

MHC Tetramers and Monomers for Immuno-Oncology and Autoimmunity Drug Discovery MHC Tetramers and Monomers for Immuno-Oncology and Autoimmunity Drug Discovery Your Partner in Drug Discovery and Research MHC Tetramer Background T-Cell Receptors recognize and bind to complexes composed

More information

Rabbit Polyclonal antibody to NFkB p65 (v-rel reticuloendotheliosis viral oncogene homolog A (avian))

Rabbit Polyclonal antibody to NFkB p65 (v-rel reticuloendotheliosis viral oncogene homolog A (avian)) Datasheet GeneTex, Inc : Toll Free 1-877-GeneTex (1-877-436-3839) Fax:1-949-309-2888 info@genetex.com GeneTex International Corporation : Tel:886-3-6208988 Fax:886-3-6208989 infoasia@genetex.com Date :

More information

Chapter 35 Active Reading Guide The Immune System

Chapter 35 Active Reading Guide The Immune System Name: AP Biology Mr. Croft Chapter 35 Active Reading Guide The Immune System Section 1 Phagocytosis plays an important role in the immune systems of both invertebrates and vertebrates. Review the process

More information

The Major Histocompatibility Complex (MHC)

The Major Histocompatibility Complex (MHC) The Major Histocompatibility Complex (MHC) An introduction to adaptive immune system before we discuss MHC B cells The main cells of adaptive immune system are: -B cells -T cells B cells: Recognize antigens

More information

Basic Immunology. Lecture 5 th and 6 th Recognition by MHC. Antigen presentation and MHC restriction

Basic Immunology. Lecture 5 th and 6 th Recognition by MHC. Antigen presentation and MHC restriction Basic Immunology Lecture 5 th and 6 th Recognition by MHC. Antigen presentation and MHC restriction Molecular structure of MHC, subclasses, genetics, functions. Antigen presentation and MHC restriction.

More information

PRODUCT INFORMATION & MANUAL

PRODUCT INFORMATION & MANUAL PRODUCT INFORMATION & MANUAL 0.4 micron for Overall Exosome Isolation (Cell Media) NBP2-49826 For research use only. Not for diagnostic or therapeutic procedures. www.novusbio.com - P: 303.730.1950 - P:

More information

TSH Receptor Monoclonal Antibody (49) Catalog Number MA3-218 Product data sheet

TSH Receptor Monoclonal Antibody (49) Catalog Number MA3-218 Product data sheet Website: thermofisher.com Customer Service (US): 1 800 955 6288 ext. 1 Technical Support (US): 1 800 955 6288 ext. 441 TSH Receptor Monoclonal Antibody (49) Catalog Number MA3-218 Product data sheet Details

More information

CHAPTER 18: Immune System

CHAPTER 18: Immune System CHAPTER 18: Immune System 1. What are four characteristics of the specific immune system? a. b. c. d. 2. List the two main types of defense mechanisms and briefly describe features of each. 3. Give examples

More information

Third line of Defense

Third line of Defense Chapter 15 Specific Immunity and Immunization Topics -3 rd of Defense - B cells - T cells - Specific Immunities Third line of Defense Specific immunity is a complex interaction of immune cells (leukocytes)

More information

10/18/2012. A primer in HLA: The who, what, how and why. What?

10/18/2012. A primer in HLA: The who, what, how and why. What? A primer in HLA: The who, what, how and why What? 1 First recognized in mice during 1930 s and 1940 s. Mouse (murine) experiments with tumors Independent observations were made in humans with leukoagglutinating

More information

Superior Fluorescent Labeling Dyes Spanning the Full Visible Spectrum...1. Trademarks: HiLyte Fluor (AnaSpec, Inc.)

Superior Fluorescent Labeling Dyes Spanning the Full Visible Spectrum...1. Trademarks: HiLyte Fluor (AnaSpec, Inc.) Table of Contents Fluor TM Labeling Dyes Superior Fluorescent Labeling Dyes Spanning the Full Visible Spectrum....1 Fluor TM 405 Dye, an Excellent Alternative to Alexa Fluor 405 & DyLight 405....2 Fluor

More information

Phase of immune response

Phase of immune response Antigen and antigen recognition by lymphocytes Antigen presentation to T lymphocytes Sanipa Suradhat Department of Veterinary Microbiology Faculty of Veterinary Science Phase of immune response 1 Phase

More information

Downloaded by on April 28, 2018 https://pubs.acs.org Publication Date: April 24, 1984 doi: /bk

Downloaded by on April 28, 2018 https://pubs.acs.org Publication Date: April 24, 1984 doi: /bk 1 Virus-Receptor Interactions BERNARD N. FIELDS Department of Microbiology and Molecular Genetics, Harvard Medical School, and Department of Medicine (Infectious Disease), Brigham and Women's Hospital,

More information

Pearson r = P (one-tailed) = n = 9

Pearson r = P (one-tailed) = n = 9 8F4-Specific Lysis, % 1 UPN1 UPN3 8 UPN7 6 Pearson r =.69 UPN2 UPN5 P (one-tailed) =.192 4 UPN8 n = 9 2 UPN9 UPN4 UPN6 5 1 15 2 25 8 8F4, % Max MFI Supplementary Figure S1. AML samples UPN1-UPN9 show variable

More information

Ex vivo Human Antigen-specific T Cell Proliferation and Degranulation Willemijn Hobo 1, Wieger Norde 1 and Harry Dolstra 2*

Ex vivo Human Antigen-specific T Cell Proliferation and Degranulation Willemijn Hobo 1, Wieger Norde 1 and Harry Dolstra 2* Ex vivo Human Antigen-specific T Cell Proliferation and Degranulation Willemijn Hobo 1, Wieger Norde 1 and Harry Dolstra 2* 1 Department of Laboratory Medicine - Laboratory of Hematology, Radboud University

More information

immunity defenses invertebrates vertebrates chapter 48 Animal defenses --

immunity defenses invertebrates vertebrates chapter 48 Animal defenses -- defenses Animal defenses -- immunity chapter 48 invertebrates coelomocytes, amoebocytes, hemocytes sponges, cnidarians, etc. annelids basophilic amoebocytes, acidophilic granulocytes arthropod immune systems

More information

Transplantation. Immunology Unit College of Medicine King Saud University

Transplantation. Immunology Unit College of Medicine King Saud University Transplantation Immunology Unit College of Medicine King Saud University Objectives To understand the diversity among human leukocyte antigens (HLA) or major histocompatibility complex (MHC) To know the

More information

Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4

Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4 Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4 Ankita Garg, Rodney Trout and Stephen A. Spector,,* Department

More information

Product Datasheet. Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) NBP Kit. Unit Size: 1 Kit. Storage is content dependent.

Product Datasheet. Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) NBP Kit. Unit Size: 1 Kit. Storage is content dependent. Product Datasheet Human, Mouse, Rat RelA/NFkB p65 ELISA Kit (Colorimetric) NBP2-29661-1Kit Unit Size: 1 Kit Storage is content dependent. Publications: 76 Protocols, Publications, Related Products, Reviews,

More information

Profiling HLA motifs by large scale peptide sequencing Agilent Innovators Tour David K. Crockett ARUP Laboratories February 10, 2009

Profiling HLA motifs by large scale peptide sequencing Agilent Innovators Tour David K. Crockett ARUP Laboratories February 10, 2009 Profiling HLA motifs by large scale peptide sequencing 2009 Agilent Innovators Tour David K. Crockett ARUP Laboratories February 10, 2009 HLA Background The human leukocyte antigen system (HLA) is the

More information

Overview: The immune responses of animals can be divided into innate immunity and acquired immunity.

Overview: The immune responses of animals can be divided into innate immunity and acquired immunity. GUIDED READING - Ch. 43 - THE IMMUNE SYSTEM NAME: Please print out these pages and HANDWRITE the answers directly on the printouts. Typed work or answers on separate sheets of paper will not be accepted.

More information

Third line of Defense. Topic 8 Specific Immunity (adaptive) (18) 3 rd Line = Prophylaxis via Immunization!

Third line of Defense. Topic 8 Specific Immunity (adaptive) (18) 3 rd Line = Prophylaxis via Immunization! Topic 8 Specific Immunity (adaptive) (18) Topics - 3 rd Line of Defense - B cells - T cells - Specific Immunities 1 3 rd Line = Prophylaxis via Immunization! (a) A painting of Edward Jenner depicts a cow

More information

Supplemental Figure 1. Cell-bound Cetuximab reduces EGFR staining intensity. Blood

Supplemental Figure 1. Cell-bound Cetuximab reduces EGFR staining intensity. Blood Antibody-mediated depletion of CD19-CAR T cells Supplemental 1 Supplemental Materials Supplemental Figure 1. Supplemental Figure 1. Cell-bound Cetuximab reduces EGFR staining intensity. Blood cells were

More information

HLA and antigen presentation. Department of Immunology Charles University, 2nd Medical School University Hospital Motol

HLA and antigen presentation. Department of Immunology Charles University, 2nd Medical School University Hospital Motol HLA and antigen presentation Department of Immunology Charles University, 2nd Medical School University Hospital Motol MHC in adaptive immunity Characteristics Specificity Innate For structures shared

More information

HIV-1 p24 ELISA Pair Set Cat#: orb54951 (ELISA Manual)

HIV-1 p24 ELISA Pair Set Cat#: orb54951 (ELISA Manual) HIV-1 p24 ELISA Pair Set Cat#: orb54951 (ELISA Manual) BACKGROUND Human Immunodeficiency Virus ( HIV ) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to

More information

Significance of the MHC

Significance of the MHC CHAPTER 7 Major Histocompatibility Complex (MHC) What is is MHC? HLA H-2 Minor histocompatibility antigens Peter Gorer & George Sneell (1940) Significance of the MHC role in immune response role in organ

More information

CATALOG OF ANTIBODIES FOR IMMUNOLOGY

CATALOG OF ANTIBODIES FOR IMMUNOLOGY CATALOG OF ANTIBODIES FOR IMMUNOLOGY Table of Contents Innate Immunity... 2-8 Cytokines... 2-4 Chemokines... 4-6 Toll-Like Receptors... 7-8 Adaptive Immunity...9-13 B Cells...9-10 T Cells...11-12 MHCs...

More information

Immunology Lecture 4. Clinical Relevance of the Immune System

Immunology Lecture 4. Clinical Relevance of the Immune System Immunology Lecture 4 The Well Patient: How innate and adaptive immune responses maintain health - 13, pg 169-181, 191-195. Immune Deficiency - 15 Autoimmunity - 16 Transplantation - 17, pg 260-270 Tumor

More information

The Generation of Specific Immunity

The Generation of Specific Immunity The Generation of Specific Immunity Antibody structure! Antibodies classified by specificity (antigen, binding site) and class (general structure, function)! Differences in variable regions produce different

More information

Physiology Unit 3. ADAPTIVE IMMUNITY The Specific Immune Response

Physiology Unit 3. ADAPTIVE IMMUNITY The Specific Immune Response Physiology Unit 3 ADAPTIVE IMMUNITY The Specific Immune Response In Physiology Today The Adaptive Arm of the Immune System Specific Immune Response Internal defense against a specific pathogen Acquired

More information

Fast and Easy Isolation of T Cells

Fast and Easy Isolation of T Cells Fast and Easy Isolation of T Cells Isolate T Cells In As Little As 25 Minutes Isolate whole T cell populations as well as various T cell subsets with high purity and recovery using the fast and easy T

More information

EBV Infection and Immunity. Andrew Hislop Institute for Cancer Studies University of Birmingham

EBV Infection and Immunity. Andrew Hislop Institute for Cancer Studies University of Birmingham EBV Infection and Immunity Andrew Hislop Institute for Cancer Studies University of Birmingham EBV Introduction Large ds DNA virus Spread by saliva contact Lifelong infection Predominantly B-lymphotropic

More information

Immunology - Lecture 2 Adaptive Immune System 1

Immunology - Lecture 2 Adaptive Immune System 1 Immunology - Lecture 2 Adaptive Immune System 1 Book chapters: Molecules of the Adaptive Immunity 6 Adaptive Cells and Organs 7 Generation of Immune Diversity Lymphocyte Antigen Receptors - 8 CD markers

More information

Attribution: University of Michigan Medical School, Department of Microbiology and Immunology

Attribution: University of Michigan Medical School, Department of Microbiology and Immunology Attribution: University of Michigan Medical School, Department of Microbiology and Immunology License: Unless otherwise noted, this material is made available under the terms of the Creative Commons Attribution

More information

Pro5 MHC Pentamers. Dr. Jeremy Fry. Copyright ProImmune Limited All Rights Reserved

Pro5 MHC Pentamers. Dr. Jeremy Fry. Copyright ProImmune Limited All Rights Reserved Pro5 MHC Pentamers Dr. Jeremy Fry Pro5 MHC Pentamers The most consistent technology for detecting antigenspecific T cells The most-cited commercially available MHC Multimer Used by most-leading academic

More information

Novocastra Liquid Mouse Monoclonal Antibody Myeloperoxidase

Novocastra Liquid Mouse Monoclonal Antibody Myeloperoxidase Novocastra Liquid Mouse Monoclonal Antibody Myeloperoxidase Product Code: NCL-L-MYELO Leica Biosystems Newcastle Ltd Balliol Business Park West Benton Lane Newcastle Upon Tyne NE12 8EW United Kingdom (

More information

CD25-PE (BD Biosciences) and labeled with anti-pe-microbeads (Miltenyi Biotec) for depletion of CD25 +

CD25-PE (BD Biosciences) and labeled with anti-pe-microbeads (Miltenyi Biotec) for depletion of CD25 + Supplements Supplemental Materials and Methods Depletion of CD25 + T-cells from PBMC. Fresh or HD precultured PBMC were stained with the conjugate CD25-PE (BD Biosciences) and labeled with anti-pe-microbeads

More information

Test Bank for Basic Immunology Functions and Disorders of the Immune System 4th Edition by Abbas

Test Bank for Basic Immunology Functions and Disorders of the Immune System 4th Edition by Abbas Test Bank for Basic Immunology Functions and Disorders of the Immune System 4th Edition by Abbas Chapter 04: Antigen Recognition in the Adaptive Immune System Test Bank MULTIPLE CHOICE 1. Most T lymphocytes

More information

Phospho-AKT Sampler Kit

Phospho-AKT Sampler Kit Phospho-AKT Sampler Kit E 0 5 1 0 0 3 Kits Includes Cat. Quantity Application Reactivity Source Akt (Ab-473) Antibody E021054-1 50μg/50μl IHC, WB Human, Mouse, Rat Rabbit Akt (Phospho-Ser473) Antibody

More information

SIV p27 ANTIGEN CAPTURE ASSAY

SIV p27 ANTIGEN CAPTURE ASSAY SIV p27 ANTIGEN CAPTURE ASSAY Enzyme Immunoassay for the detection of Simian Immunodeficiency Virus (SIV) p27 in tissue culture media Catalog #5436 and #5450 Version 6; 12/2012 ABL PRODUCTS AND SERVICES

More information

Supporting Information

Supporting Information Supporting Information Pang et al. 10.1073/pnas.1322009111 SI Materials and Methods ELISAs. These assays were performed as previously described (1). ELISA plates (MaxiSorp Nunc; Thermo Fisher Scientific)

More information

White Blood Cells (WBCs)

White Blood Cells (WBCs) YOUR ACTIVE IMMUNE DEFENSES 1 ADAPTIVE IMMUNE RESPONSE 2! Innate Immunity - invariant (generalized) - early, limited specificity - the first line of defense 1. Barriers - skin, tears 2. Phagocytes - neutrophils,

More information

RAISON D ETRE OF THE IMMUNE SYSTEM:

RAISON D ETRE OF THE IMMUNE SYSTEM: RAISON D ETRE OF THE IMMUNE SYSTEM: To Distinguish Self from Non-Self Thereby Protecting Us From Our Hostile Environment. Innate Immunity Acquired Immunity Innate immunity: (Antigen nonspecific) defense

More information

A mechanism for glycoconjugate vaccine activation of the adaptive immune system and its implications for vaccine design

A mechanism for glycoconjugate vaccine activation of the adaptive immune system and its implications for vaccine design A mechanism for glycoconjugate vaccine activation of the adaptive immune system and its implications for vaccine design Fikri Y. Avci 1,2, Xiangming Li 3, Moriya Tsuji 3, Dennis L. Kasper 1,2* Supplementary

More information

Our T cell epitope test service offers you:

Our T cell epitope test service offers you: Our T cell epitope test service offers you: high throughput peptide discovery fast lead-finding maximum of epitopes in a minimum of blood saving effort and costs on developing your own platforms custom-made

More information

HLA and antigen presentation. Department of Immunology Charles University, 2nd Medical School University Hospital Motol

HLA and antigen presentation. Department of Immunology Charles University, 2nd Medical School University Hospital Motol HLA and antigen presentation Department of Immunology Charles University, 2nd Medical School University Hospital Motol MHC in adaptive immunity Characteristics Specificity Innate For structures shared

More information

Human Cathepsin D ELISA Kit

Human Cathepsin D ELISA Kit GenWay Biotech, Inc. 6777 Nancy Ridge Drive San Diego, CA 92121 Phone: 858.458.0866 Fax: 858.458.0833 Email: techline@genwaybio.com http://www.genwaybio.com Human Cathepsin D ELISA Kit Catalog No. GWB-J4JVV9

More information

Human HBcAb IgM ELISA kit

Human HBcAb IgM ELISA kit Human HBcAb IgM ELISA kit Catalog number: NR-R10163 (96 wells) The kit is designed to qualitatively detect HBcAb IgM in human serum or plasma. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PURPOSES

More information

I. Critical Vocabulary

I. Critical Vocabulary I. Critical Vocabulary A. Immune System: a set of glands, tissues, cells, and dissolved proteins that combine to defend against non-self entities B. Antigen: any non-self chemical that triggers an immune

More information

Single and Multiplex Immunohistochemistry

Single and Multiplex Immunohistochemistry Single and Multiplex Immunohistochemistry Steve Westra, BS Reagent Product Specialist Leica Biosystems IHC Theory Polyclonal vs Monoclonal Polyclonal reagents Detect a multitude of epitopes Batch to batch

More information

Foundations in Microbiology

Foundations in Microbiology Foundations in Microbiology Fifth Edition Talaro Chapter 15 The Acquisition of Specific Immunity and Its Applications Chapter 15 2 Chapter Overview 1. Development of the Dual Lymphocyte System 2. Entrance

More information

Novocastra Liquid Mouse Monoclonal Antibody MyoD1 (Rhabdomyosarcoma Marker)

Novocastra Liquid Mouse Monoclonal Antibody MyoD1 (Rhabdomyosarcoma Marker) Novocastra Liquid Mouse Monoclonal Antibody MyoD1 (Rhabdomyosarcoma Marker) Product Code: NCL-L-MyoD1 Leica Biosystems Newcastle Ltd Balliol Business Park West Benton Lane Newcastle Upon Tyne NE12 8EW

More information

human Total Cathepsin B Catalog Number: DY2176

human Total Cathepsin B Catalog Number: DY2176 human Total Cathepsin B Catalog Number: DY2176 This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Total

More information

Significance of the MHC

Significance of the MHC CHAPTER 8 Major Histocompatibility Complex (MHC) What is is MHC? HLA H-2 Minor histocompatibility antigens Peter Gorer & George Sneell (1940) Significance of the MHC role in immune response role in organ

More information

Attribution: University of Michigan Medical School, Department of Microbiology and Immunology

Attribution: University of Michigan Medical School, Department of Microbiology and Immunology Attribution: University of Michigan Medical School, Department of Microbiology and Immunology License: Unless otherwise noted, this material is made available under the terms of the Creative Commons Attribution

More information

Commercially available HLA Class II tetramers (Beckman Coulter) conjugated to

Commercially available HLA Class II tetramers (Beckman Coulter) conjugated to Class II tetramer staining Commercially available HLA Class II tetramers (Beckman Coulter) conjugated to PE were combined with dominant HIV epitopes (DRB1*0101-DRFYKTLRAEQASQEV, DRB1*0301- PEKEVLVWKFDSRLAFHH,

More information

MagCapture Exosome Isolation Kit PS Q&A

MagCapture Exosome Isolation Kit PS Q&A MagCapture Exosome Isolation Kit PS Q&A Specifications and performance P.1 Comparison of the conventional method P.2 Operation methods and composition P.4 Amount of starting sample P.5 Analysis after exosomes

More information

CYTOMEGALOVIRUS (CMV) IgM ELISA Kit Protocol

CYTOMEGALOVIRUS (CMV) IgM ELISA Kit Protocol CYTOMEGALOVIRUS (CMV) IgM ELISA Kit Protocol (Cat. No.:EK-310-91) 330 Beach Road, Burlingame CA Tel: 650-558-8898 Fax: 650-558-1686 E-Mail: info@phoenixpeptide.com www.phoenixpeptide.com INTENDED USE The

More information

Topics in Parasitology BLY Vertebrate Immune System

Topics in Parasitology BLY Vertebrate Immune System Topics in Parasitology BLY 533-2008 Vertebrate Immune System V. Vertebrate Immune System A. Non-specific defenses against pathogens 1. Skin - physical barrier a. Tough armor protein KERATIN b. Surface

More information

Immune system. Aims. Immune system. Lymphatic organs. Inflammation. Natural immune system. Adaptive immune system

Immune system. Aims. Immune system. Lymphatic organs. Inflammation. Natural immune system. Adaptive immune system Aims Immune system Lymphatic organs Inflammation Natural immune system Adaptive immune system Major histocompatibility complex (MHC) Disorders of the immune system 1 2 Immune system Lymphoid organs Immune

More information