Muse Assays for Cell Analysis
|
|
- Gwendoline Edwards
- 5 years ago
- Views:
Transcription
1 Muse Assays for Cell Analysis
2 Multiple Assay Outputs for Cell Analysis Cell Health Cell Signalling Immunology Muse Count & Viability Kit Muse Cell Cycle Kit Muse Annexin V & Dead Cell Kit Muse Caspase 3,7 Kit Muse MultiCaspase Kit Muse MitoPotential Kit Muse Nitric Oxide Kit Muse Oxidative Stress Kit Muse Ki67 Proliferation Kit Muse LC3 Autophagy (Ab Based) Muse LC3 Autophagy (Reporter Cell Line) Muse H2A.X Activation Dual Detection Kit Muse MAPK Activation Dual Detection Kit Muse EGFR-RTK Activation Dual Detection Kit Muse PI3 Activation Dual Detection Kit Muse Bcl-2 Activation Dual Detection Kit Muse Multi-Color DNA Damage Kit Muse PI3K/MAPK Activation Dual Detection Kit Muse Human CD4 T Cell Kit Muse Human CD8 T Cell Kit Muse Human B Cell Kit Muse Human CD25 Lymphocyte Kit Muse Human CD69 Lymphocyte Kit Nucleic Acid Muse SmartFlare Live Cell RNA Detection Kit 2
3 Cell Health Assays
4 Muse Count and Viability Assay MCH Tests MCH Tests MCH for difficult samples Uses 2 dyes to assess cellular viability Viable cells have intact membranes and are nonpermeable to charged dyes Dead cells have permeability to charged dyes Inclusion of a nucleated cell dye allows identification of all cells over debris or non-nucleated cells Muse Assay Features/Benefits: Total prep time <15 min (simple Mix-and-Read assay) OK for wide variety of cell types (adherent and suspension cell lines) Wide concentration range of sample OK Minimal sample volume required Non-Viable Cell Not a Cell Viable Cell Kit detects: Concentrations of viable cells, dead cells and total cells. % viability of cells Distinction between late apoptotic and live cells Muse Count and Viability Reagent 4
5 Muse Annexin V & Dead Cell Assay (MCH100105) Determines number and percentage of cells undergoing apoptosis (early apoptotic cells) by the binding of Annexin V-PE to phosphatidylserine, a membrane component translocated to the cell surface during apoptosis. Determines number of late apoptotic and dead cells based on loss of membrane integrity Inclusion of 7AAD in the assay allows for the detection of dead/late apoptotic cells with permeabilized membranes. Muse Annexin V & Dead Cell Reagent 5
6 Muse Cell Cycle Assay (MCH100106) Kit detects percentage of cells in various stages of cell cycle Propidium iodide intercalates into the base pairs of dsdna Fluorescent intensity of a stained cell is directly proportional to the DNA content of the cell, and consequently, reflects phase of cell cycle Muse Cell Cycle Reagent 6
7 Muse Caspase-3/7 Assay (MCH100108) Kit detects percentage and concentration of cells in various stages of apoptosis, based on Caspase-3/7 activity, in combination with a dead cell dye. Kit contains Caspase 3/7 Reagent which contains a DNA binding dye linked to a Caspase 3/7 substrate. When bound to DEVD substrate the dye s ability to electrostatically bind to DNA is inactivated. Cleavage by active caspases results in release of the dye and translocation to the nucleus Upon binding to DNA, dye becomes highly fluorescent Inclusion of 7AAD in the assay allows for the detection of dead/late apoptotic cells with permeabilized membranes. NucView Caspase Substrate 7 7AAD Assay Buffer
8 Muse Multi-Caspase Assay (MCH100109) Kit detects percentage and concentration of cells in various stages of apoptosis, based on Multiple Caspase activity, in combination with a dead cell dye. Muse Multi-Caspase Assays is a mix and read assay for Pan caspase, that can detect the presence of multiple caspases (Caspase 1,3,4,5,6, 7, 8 & 9) in combination with a dead cell dye. Assay uses a Fluorescent-Labeled Inhibitor of Caspases (FLICA) Binds to caspases which are activated during apoptosis; resulting fluorescent signal is proportional to the number of active caspases in cell. Also detects cells in late apoptosis based on staining with a membrane impermeant DNA intercalator (inclusion of dead cell dye 7AAD) MultiCaspase Reagent 7AAD 10 X Assay Buffer + 1X PBS 8 Anhydrous DMSO
9 Muse MitoPotential Assay (MCH100110) Muse MitoPotential Assay is a simple no-wash assay that provides percentage and concentration of cells demonstrating mitochondrial membrane depolarization and cell death. Assay is based on detection of mitochondrial potential changes using Muse MitoPotential Reagent which is a cationic lipophilic dye. High membrane potential drives accumulation of dye within inner membrane of intact mitochondria resulting in high fluorescence. Depolarized cells are detected by decreased fluorescence. 7AAD enters cells that are compromised in dead or late apoptotic cells. 9 Muse MitoPotential Dye 7AAD 1 X Assay Buffer
10 Muse Nitric Oxide Kit (MCH100112) Assay Prinicple: Simplified assay on Muse to detect Nitric Oxide (NO) and Nitric Oxide Synthase (NOS) based activity in combination with cell death. Based on DAX-J2 Orange a novel, non-fluorescent dye that is cell permeable and becomes fluorescent product upon NO oxidation. Photostable, & ph independent advantage over other probes. Inclusion of 7AAD in the assay allows for the detection of dead/late apoptotic cells with permeabilized membranes. NO(-) Dead cells Negative (Live) cells RAW Cells treated with LPS + IFNɣ and analyzed with Muse Nitric Oxide Assay NO(+) Dead cells NO(+) Live cells Nitric Oxide Detection Reagent 7AAD Reagent 1X Assay Buffer 1X Assay Buffer BA 10
11 Muse Oxidative Stress Kit (MCH100111) Simplified assay on Muse for the detection of ROS (reactive oxygen species) in cells, esp superoxide radicals. Based on Dihydroethidium, a well characterized and cell permeable reagent which exhibits blue fluorescence in cytosol, but red fluorescence when oxidized and bound to DNA. Simultaneously determines the count and percentage of cells undergoing oxidative stress Negative cells ROS(+) cells Optional overlay feature for better comparison purpose Muse Oxidative Stress Reagent 1X Assay Buffer 1X Assay Buffer BA 11
12 Muse Ki67 Proliferation Kit (MCH100114). Ki67 is a prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2, and M phases), but is absent in the resting G0 phase. This characteristic makes Ki67 a good marker for the identification of proliferating cells. Uses assay reagent that is specific to Ki67 Simplified single color assay on Muse to detect proliferation index (% of cells proliferating in a population) Muse Ki67 Antibody Muse Isotype Control 5X Assay Buffer 5X Fixation Buffer Permeabilization buffer Optional histogram overlay of the isotype control (grey) 12
13 Autophagy 2 Assays 1) Muse Autophagy LC3-antibody based Kit (MCH200109) LC3 is a key protein indicated in autophagy which regulates traffic between autophagosome to lysosome. Provides the reagent for the disruption of the cell plasma membrane using a proprietary selective permeabilization solution. The selective permeabilization solution will extract cytosolic LC3 by flushing away during washing steps. LC3 translocated into the autophagosome is protected from the extraction and remains intact inside autophagosome, thereby allowing its fluorescence to be measured by flow cytometry. Anti-LC3 mouse monoclonal antibody conjugated to Alexa Fluor 555 is used to measure and track the levels of LC3 within the cell 2) Muse Autophagy Reporter Cell Line 13 (MCH200110) mrfp-lc3 reporter cell line for comparative studies constitutively produces LC3 Autophagy LC3-antibody based Kit 1) Muse LC3 Antibody Muse Reagent Detection Pack 5X Assay Buffer 2) mrfp-lc3 Reporter Cell Line plus Autophagy Reagent Pack
14 Cell Signalling Assays P P P
15 Muse H2A.X Activation Dual Detection Kit (MCH200101) This two color kit is designed to detect the extent of Histone H2A.X pathway activation by measuring H2A.X phosphorylation relative to the total H2A.X expression in any given cell population. Includes two directly conjugated antibodies, a phosphospecific anti-phospho-histone H2A.X (Ser139)-Alexa Fluor 555 and an anti-histone H2A.XPECy5 conjugated antibody to measure total levels of Histone H2A.X. The levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of H2A.X activation after stimulation. A dot plot displaying cells which are inactivated, non-expressing, and activated. A bar graph illustration of the same data, calculating the expression of each cell population as a percentage (%). Anti-phospho-Histone H2A.X (Ser139), Alexa Fluor 555; Anti-H2A.X, PECy5 5 X Assay Buffer 1 X Fixation Buffer 1 X Permeabilization Buffer 15
16 Muse MAPK Activation Dual Detection Kit (MCH200104) This two color kit is designed to measure the extent of MAPK phosphorylation relative to the total MAPK expression in any given cell population. Two directly conjugated antibodies, a phosphospecific anti-phospho-erk1/2 Thr202/Tyr204, Thr185/Tyr187)-Phycoerythrin and an anti-erk1/2- PECy5 conjugated antibody to measure total levels of ERK. The levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of MAPK activation after stimulation. Moreover, simultaneous measurement of both total and phospho-erk1/2 confirms target specificity of the phosphorylation event. A dot plot displaying cells which are inactivated, non-expressing, and activated. A bar graph illustration of the same data, calculating the expression of each cell population as a percentage (%). Anti-phospho-ERK1/2 (Thr202/Tyr204, Thr185/Tyr187), PE; Anti-ERK1/2, PECy5 5 X Assay Buffer 1 X Fixation Buffer 1 X Permeabilization Buffer 16
17 Muse EGFR-RTK Activation Dual Detection Kit (MCH200102) This two color kit is designed to detect the extent of EGFR pathway activation by measuring EGFR phosphorylation relative to the total EGFR expression in any given cell population. Two directly conjugated antibodies, a phosphospecific anti-phospho-egfr (Tyr1173)-Alexa Fluor 555 and an anti-egfr-pecy5 conjugated antibody to measure total levels of EGFR expression. The levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of EGFR activation after stimulation. Moreover, simultaneous measurement of both total and phospho-egfr confirms target specificity of the phosphorylation event. A dot plot displaying cells which are inactivated, non-expressing, and activated. A bar graph illustration of the same data, calculating the expression of each cell population as a percentage (%). 17 Anti-phospho-EGFR (Tyr1173), Alexa Fluor 555; Anti-EGFR, PECy5 5 X Assay Buffer 1 X Fixation Buffer 1 X Permeabilization Buffer
18 Muse PI3K Activation Dual Detection Kit (MCH200103) This two color kit is designed to detect the extent of Akt phosphorylation relative to the total Akt expression in any given cell population. Two directly conjugated antibodies, a phosphospecific anti-phospho-akt (Ser473), Alexa Fluor 555 and an anti-akt, PECy5 conjugated antibody to measure total levels of Akt. The levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of PI3K activation after stimulation. Moreover, simultaneous measurement of both total and phospho-akt confirms target specificity of the phosphorylation event. A dot plot displaying cells which are inactivated, non-expressing, and activated. A bar graph illustration of the same data, calculating the expression of each cell population as a percentage (%). Anti-phospho-Akt (Ser473), Alexa Fluor 555 Anti-Akt/PKB, PECy5 5 X Assay Buffer 1 X Fixation Buffer 1 X Permeabilization Buffer 18
19 Muse Bcl-2 Activation Dual Detection Kit (MCH200105) This two color kit is designed to detect the extent of Bcl-2 pathway activation by measuring Bcl-2 phosphorylation relative to the total Bcl-2 expression in any given cell population Two directly conjugated antibodies, a phosphospecific anti-phospho-bcl-2 (Ser70)-Alexa Fluor 555 and an anti-bcl-2-pecy5 conjugated antibody to measure total levels of Bcl-2 expression. The levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of Bcl-2 activation after treatment. Moreover, simultaneous measurement of both total and phospho-bcl-2 confirms target specificity of the phosphorylation event. A dot plot displaying cells which are inactivated, non-expressing, and activated. A bar graph illustration of the same data, calculating the expression of each cell population as a percentage (%). Anti-phospho-Bcl-2 (Ser70)-Alexa Fluor 555 Anti-Bcl-2-PECy5 5 X Assay Buffer 1 X Fixation Buffer 1 X Permeabilization Buffer 19
20 Muse Multi-Color DNA Damage Kit (MCH200107) The kit measures the levels of two key signaling nodes along the DNA damage signaling pathway, H2A.X and Ataxiatelangiectasia mutated kinase (ATM). ATM is the main kinase activated in response to doublestranded DNA breaks. Once activated, ATM phosphorylates a number of downstream factors, including Histone H2A.X. Phosphorylation of H2A.X, at serine 139 by ATM is an important indicator of DNA damage. Two directly conjugated antibodies, a phosphospecific ATM (Ser1981)-PE and a phospho-specific Histone H2A.X-PECy5 conjugated antibody measure the extent of DNA damage in testing samples. This two color kit is designed to detect the phosphorylation state of ATM and Histone H2A.X simultaneously by flow analysis. 20 Anti-phospho-ATM (Ser1981), PE Anti-phospho-Histone H2A.X (Ser139), PECy5 5 X Assay Buffer 1 X Fixation Buffer 1 X Permeabilization Buffer
21 Muse PI3K/MAPK Dual Pathway Activation Kit (MCH200108) Evaluation of both the PI3K and MAPK signaling pathways simultaneously. There is evidence that these two key signaling pathways cross-talk downstream along the signaling cascade, which can potentially influence the activation of one another. Two directly conjugated antibodies, a phospho-specific Akt (Ser473)-Alexa Fluor 555 and a phospho-specific ERK1/2 (Thr202/Tyr204, Thr185/Tyr187)-PECy5 conjugated antibody assess the activation of both the PI3K and MAPK signaling pathways simultaneously in testing samples. 21 Anti-phospho-Akt (Ser473), Alexa Fluor 555 Anti-phospho-ERK1/2 (Thr202/Tyr204,Thr185/Tyr187), PECy5 5 X Assay Buffer 1 X Fixation Buffer 1 X Permeabilization Buffer
22 Immunology Assays CD8 T cells Mono CD4 T cells B cells Tregs NK Cells
23 Muse Human CD4 T Cell Kit (MIM100101) Detection and identification of lymphocytes and CD4 T lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. The CD4 antibody identifies human helper/inducer CD4+ T cell (HLA Class II reactive) and recognizes a 60,000 Da surface antigen. Monocytes also express CD4 but at a lower density, and have no co-expression of the other antibodies present in the anti-lymphocyte cocktail; hence can be distinguished from CD4 T cells in this kit. The assay uses an anti-lymphocyte cocktail that identifies the lymphocyte population, and a CD4 antibody that binds to the CD4 cells. Non-CD4 Lymphocytes Negative CD4 T Lymphocytes CD4 Non- Lymphocytes Muse Human CD4 T Cell Cocktail 1 X Assay Buffer BA 1 X Lysing Solution 23
24 Muse Human CD8 T Cell Kit (MIM100102) Detection and identification of lymphocytes and CD8 T lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. The CD8 antibody allows the identification of CD8, a 68- kda transmembrane glycoprotein expressed by class I major histocompatibility complex restricted, mature suppressor/cytotoxic T cells, the great majority of cortical thymocytes and approximately 30% of medullary thymocytes. In addition a proportion of γδ T cells and NK cells express CD8. Inclusion of several proprietary antibodies in the anti-lymphocyte cocktail allows for the unique identification of the CD8 cytotoxic T cells. The assay uses an anti-lymphocyte cocktail that identifies the lymphocyte population, and a CD8 antibody that binds to the CD8 cells. CD8 T Lymphocytes PROFILE CD8 (-) T & B Lymphocytes Negative CD8 CD8(+) T Lymphocytes CD8 (+) Cells Muse Human CD8 T Cell Cocktail 1 X Assay Buffer BA 1 X Lysing Solution 24
25 Muse Human B Cell Kit (MIM100103) Detection and identification of lymphocytes and CD19 B lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. CD19 is considered to be a characteristic B cell marker and therefore commonly used in the routine immunophenotyping of B cells. Monoclonal antibodies clustered as CD19 detect all peripheral blood B cells. The assay uses an anti-lymphocyte cocktail that identifies the lymphocyte population, and a CD19 antibody that binds to the B cell population. Non-B Lymphocytes (CD19-) Negative B Lymphocytes (CD19+) Non-Lymphocytes (CD19+) Muse Human B Cell Cocktail 1 X Assay Buffer BA 1 X Lysing Solution 25
26 Muse Human Lymphocyte CD69 Kit (MIM100104) Detection and identification of lymphocytes and CD69 lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. CD69 is a disulfide-linked dimeric structure of two phosphorylated polypeptide chains (27 kda and 33 kda) that are differentially glycosylated forms of the same core protein. CD69 (also known as activation inducer molecule [AIM]), is the earliest inducible surface antigen expressed on lymphocytes after T- or B-cell activation and is absent from resting lymphocytes. The assay uses an anti-lymphocyte cocktail that identifies the total lymphocyte population, and a CD69 antibody that identifies activation marker CD69. The kit also includes an isotype control antibody. negative CD69+ Lymphocytes 26 Muse Human Lymphocyte Cocktail Human CD69-PE Antibody Isotype Control Mouse IgG1-PE 1 X Assay Buffer BA 1 X Lysing Solution
27 Muse Human Lymphocyte CD25 Kit (MIM100105) Detection and identification of lymphocytes and CD25 lymphocytes in either whole blood or PBMCs using a simplified no-wash assay. CD25 (IL-2 receptor α-chain) is an mid to late activation marker expressed on the surface of activated lymphocytes including T and B lymphocytes and NK cells. It is also expressed constitutively on the surface of small subpopulations of T cells. The assay uses an anti-lymphocyte cocktail that identifies the total lymphocyte population, and a CD25 antibody that identifies activation marker CD25. The kit also includes an isotype control antibody. negative CD69+ Lymphocytes 27 Muse Human Lymphocyte Cocktail Human CD25-PE Antibody Isotype Control Mouse IgG1-PE 1 X Assay Buffer BA 1 X Lysing Solution
28 Nucleic Acid Assays
29 SmartFlare RNA Detection Probes Detection on Muse Cell Analyzer SmartFlare RNA detection reagent is a novel probe capable of detecting specific mrnas and mirnas in live cells. This technology allows for carrier-free cellular endocytosis of the reagent, followed by detection and relative quantitative analysis of RNA levels in individual cells Each SmartFlare probe consists of a gold nanoparticle conjugated to multiple copies of a doublestranded oligonucleotide, in which one strand (the reporter strand ) bears a fluorophore that is quenched by its proximity to the gold core. When the nanoparticle comes into contact with its target RNA, the target RNA binds to its complementary capture strand and displaces the reporter strand. The reporter strand, whose Cy3 fluorophore is now unquenched, fluoresces and can be detected using the Muse Cell Analyzer. SmartFlare RNA detection probes conjugated with Cyanine (Cy3) fluorescent dye are optimized on the Muse Cell Analyzer. Data generated using the Muse provides the RNA expression levels of target genes and the percentage of expressing cells. Unflared - cells are incubated in media alone Scrambled sequence unspecific SmartFlare Target - SmartFlare Cy3 probes 29
Supplementary Table; Supplementary Figures and legends S1-S21; Supplementary Materials and Methods
Silva et al. PTEN posttranslational inactivation and hyperactivation of the PI3K/Akt pathway sustain primary T cell leukemia viability Supplementary Table; Supplementary Figures and legends S1-S21; Supplementary
More informationPE Annexin V Apoptosis Detection Kit User Manual KT40001
PE Annexin V Apoptosis Detection Kit User Manual KT40001 For research use only. Not intended for diagnostic testing. a WuXi AppTec company www.abgent.com.cn PE Annexin-V Apoptosis Detection Kit Product
More informationFluorescence Microscopy
Fluorescence Microscopy Imaging Organelles Mitochondria Lysosomes Nuclei Endoplasmic Reticulum Plasma Membrane F-Actin AAT Bioquest Introduction: Organelle-Selective Stains Organelles are tiny, specialized
More informationThe Annexin V Apoptosis Assay
The Annexin V Apoptosis Assay Development of the Annexin V Apoptosis Assay: 1990 Andree at al. found that a protein, Vascular Anticoagulant α, bound to phospholipid bilayers in a calcium dependent manner.
More informationImmunology - Lecture 2 Adaptive Immune System 1
Immunology - Lecture 2 Adaptive Immune System 1 Book chapters: Molecules of the Adaptive Immunity 6 Adaptive Cells and Organs 7 Generation of Immune Diversity Lymphocyte Antigen Receptors - 8 CD markers
More informationAnnexin V-APC/7-AAD Apoptosis Kit
Annexin V-APC/7-AAD Apoptosis Kit Catalog Number KA3808 100 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4
More informationThe Biochemistry of apoptosis
The Biochemistry of apoptosis 1 1 The apoptosis is composed of multiple biochemical events 2 2 Biochemical, cellular, and molecular events in Apoptosis 1. Membrane blebbing; phosphatidyl serine exposure
More informationRayBio Annexin V-FITC Apoptosis Detection Kit
RayBio Annexin V-FITC Apoptosis Detection Kit User Manual Version 1.0 May 25, 2014 (Cat#: 68FT-AnnV-S) RayBiotech, Inc. We Provide You With Excellent Support And Service Tel:(Toll Free)1-888-494-8555 or
More informationOptimizing Intracellular Flow Cytometry:
Optimizing Intracellular Flow Cytometry: Simultaneous Detection of Cytokines and Transcription Factors An encore presentation by Jurg Rohrer, PhD, BD Biosciences 10.26.10 Outline Introduction Cytokines
More informationOptimizing Intracellular Flow Cytometry
Optimizing Intracellular Flow Cytometry Detection of Cytokines, Transcription Factors, and Phosphoprotein by Flow Cytometry Presented by Erika O Donnell, PhD, BD Biosciences 23-14876-00 Outline Basic principles
More informationSupplementary Materials
Supplementary Materials Figure S1. MTT Cell viability assay. To measure the cytotoxic potential of the oxidative treatment, the MTT [3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide] assay
More informationOptimizing Intracellular Flow Cytometry:
Optimizing Intracellular Flow Cytometry: Simultaneous Detection of Cytokines and Transcription Factors Presented by Jurg Rohrer, PhD, BD Biosciences 23-10780-00 Outline Introduction Cytokines Transcription
More informationAnnexin V-PE Apoptosis Detection Kit
Annexin V-PE Apoptosis Detection Kit Catalog Number KA0716 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...
More informationRayBio Annexin V-FITC Apoptosis Detection Kit Plus
RayBio Annexin V-FITC Apoptosis Detection Kit Plus User Manual Version 1.0 May 25, 2014 RayBio Annexin V-FITC Apoptosis (Cat#: 68FT-AnnVP-) RayBiotech, Inc. We Provide You With Excellent upport And ervice
More informationInstructions for Use. APO-AB Annexin V-Biotin Apoptosis Detection Kit 100 tests
3URGXFW,QIRUPDWLRQ Sigma TACS Annexin V Apoptosis Detection Kits Instructions for Use APO-AB Annexin V-Biotin Apoptosis Detection Kit 100 tests For Research Use Only. Not for use in diagnostic procedures.
More informationMulti-Parameter Apoptosis Assay Kit
Multi-Parameter Apoptosis Assay Kit Catalog Number KA1335 5 x 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay...
More informationCommercially available HLA Class II tetramers (Beckman Coulter) conjugated to
Class II tetramer staining Commercially available HLA Class II tetramers (Beckman Coulter) conjugated to PE were combined with dominant HIV epitopes (DRB1*0101-DRFYKTLRAEQASQEV, DRB1*0301- PEKEVLVWKFDSRLAFHH,
More informationSupporting Information. Non-thermal plasma with 2-deoxy-D-glucose synergistically induces cell death by targeting glycolysis in blood cancer cells
Supporting Information Non-thermal plasma with 2-deoxy-D-glucose synergistically induces cell death by targeting glycolysis in blood cancer cells Neha Kaushik, 1 Su Jae Lee, 2 Tae Gyu Choi 3, Ku Youn Baik,
More informationAnnexin V-FITC Apoptosis Detection Kit
ab14085 Annexin V-FITC Apoptosis Detection Kit Instructions for Use For the rapid, sensitive and accurate measurement of Apoptosis in living cells (adherent and suspension). View kit datasheet: www.abcam.com/ab14085
More informationIntroduction to pathology lecture 5/ Cell injury apoptosis. Dr H Awad 2017/18
Introduction to pathology lecture 5/ Cell injury apoptosis Dr H Awad 2017/18 Apoptosis = programmed cell death = cell suicide= individual cell death Apoptosis cell death induced by a tightly regulated
More informationAnnexin V-FITC Apoptosis Detection Kit with SYTOX
ab14086 Annexin V-FITC Apoptosis Detection Kit with SYTOX Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in living cells. This product is for research use only and
More informationPlate-Based Assay Methods for the Assessment of Cellular Health
Plate-Based Assay Methods for the Assessment of Cellular Health Andrew L. Niles, Senior Research Scientist 2012, Promega Corporation. Biological Outcomes in Cell Culture Treatment -Small molecule -Bio-molecule
More informationFor the rapid, sensitive and accurate measurement of apoptosis in various samples.
ab14082 500X Annexin V-FITC Apoptosis Detection Reagent Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in various samples. This product is for research use only and
More informationThird line of Defense
Chapter 15 Specific Immunity and Immunization Topics -3 rd of Defense - B cells - T cells - Specific Immunities Third line of Defense Specific immunity is a complex interaction of immune cells (leukocytes)
More informationIMMUNE CELL SURFACE RECEPTORS AND THEIR FUNCTIONS
LECTURE: 07 Title: IMMUNE CELL SURFACE RECEPTORS AND THEIR FUNCTIONS LEARNING OBJECTIVES: The student should be able to: The chemical nature of the cellular surface receptors. Define the location of the
More informationWhat would you observe if you fused a G1 cell with a S cell? A. Mitotic and pulverized chromosomes. B. Mitotic and compact G1 chromosomes.
What would you observe if you fused a G1 cell with a S cell? A. Mitotic and pulverized chromosomes. B. Mitotic and compact G1 chromosomes. C. Mostly non-compact G1 chromosomes. D. Compact G1 and G2 chromosomes.
More informationSuperior Fluorescent Labeling Dyes Spanning the Full Visible Spectrum...1. Trademarks: HiLyte Fluor (AnaSpec, Inc.)
Table of Contents Fluor TM Labeling Dyes Superior Fluorescent Labeling Dyes Spanning the Full Visible Spectrum....1 Fluor TM 405 Dye, an Excellent Alternative to Alexa Fluor 405 & DyLight 405....2 Fluor
More informationAcute lung injury in children : from viral infection and mechanical ventilation to inflammation and apoptosis Bern, R.A.
UvA-DARE (Digital Academic Repository) Acute lung injury in children : from viral infection and mechanical ventilation to inflammation and apoptosis Bern, R.A. Link to publication Citation for published
More informationRayBio Annexin V-Cy5 Apoptosis Detection Kit
RayBio Annexin V-Cy5 Apoptosis Detection Kit User Manual Version 1.0 Mar 20, 2014 (Cat#: 68C5-AnnV-S) RayBiotech, Inc. We Provide You With Excellent Support And Service Tel:(Toll Free)1-888-494-8555 or
More informationa Beckman Coulter Life Sciences: White Paper
a Beckman Coulter Life Sciences: White Paper An 8-color DuraClone IM panel for detection of Human blood dendritic cells by flow cytometry Nathalie Dupas 1, Snehita Sattiraju 2, Neha Girish 2, Murthy Pendyala
More informationEx vivo Human Antigen-specific T Cell Proliferation and Degranulation Willemijn Hobo 1, Wieger Norde 1 and Harry Dolstra 2*
Ex vivo Human Antigen-specific T Cell Proliferation and Degranulation Willemijn Hobo 1, Wieger Norde 1 and Harry Dolstra 2* 1 Department of Laboratory Medicine - Laboratory of Hematology, Radboud University
More informationIn vitro human regulatory T cell expansion
- 1 - Human CD4 + CD25 + CD127 dim/- regulatory T cell Workflow isolation, in vitro expansion and analysis In vitro human regulatory T cell expansion Introduction Regulatory T (Treg) cells are a subpopulation
More informationFigure S1. Western blot analysis of clathrin RNA interference in human DCs Human immature DCs were transfected with 100 nm Clathrin SMARTpool or
Figure S1. Western blot analysis of clathrin RNA interference in human DCs Human immature DCs were transfected with 100 nm Clathrin SMARTpool or control nontargeting sirnas. At 90 hr after transfection,
More informationIntrinsic cellular defenses against virus infection
Intrinsic cellular defenses against virus infection Detection of virus infection Host cell response to virus infection Interferons: structure and synthesis Induction of antiviral activity Viral defenses
More informationFigure S1. PMVs from THP-1 cells expose phosphatidylserine and carry actin. A) Flow
SUPPLEMENTARY DATA Supplementary Figure Legends Figure S1. PMVs from THP-1 cells expose phosphatidylserine and carry actin. A) Flow cytometry analysis of PMVs labelled with annexin-v-pe (Guava technologies)
More informationNature Protocols: doi: /nprot Supplementary Figure 1
Supplementary Figure 1 Traditional electronic gating strategy for analysing cell death based on A5-FITC and 7-AAD. a, Flow cytometry analysis showing the traditional two-stage electronic gating strategy
More informationProlonged mitotic arrest induces a caspase-dependent DNA damage
SUPPLEMENTARY INFORMATION Prolonged mitotic arrest induces a caspase-dependent DNA damage response at telomeres that determines cell survival Karolina O. Hain, Didier J. Colin, Shubhra Rastogi, Lindsey
More informationUnder the Radar Screen: How Bugs Trick Our Immune Defenses
Under the Radar Screen: How Bugs Trick Our Immune Defenses Session 8: Apoptosis Marie-Eve Paquet and Gijsbert Grotenbreg Whitehead Institute for Biomedical Research Myxoma virus Poxvirus Infects rabbits
More informationAssessing Autophagy with the guava easycyte Benchtop Flow Cytometer
Application Note Assessing Autophagy with the guava easycyte Benchtop Flow Cytometer Mark Santos, Kevin Su, Luke Armstrong, Angelica Olcott, Jason Whalley, and Matthew Hsu EMD Millipore Introduction Autophagy
More information- 1 - Cell types Monocytes THP-1 cells Macrophages. LPS Treatment time (Hour) IL-6 level (pg/ml)
Supplementary Table ST1: The dynamic effect of LPS on IL-6 production in monocytes and THP-1 cells after GdA treatment. Monocytes, THP-1 cells and macrophages (5x10 5 ) were incubated with 10 μg/ml of
More informationSUPPLEMENT Supplementary Figure 1: (A) (B)
SUPPLEMENT Supplementary Figure 1: CD4 + naïve effector T cells (CD4 effector) were labeled with CFSE, stimulated with α-cd2/cd3/cd28 coated beads (at 2 beads/cell) and cultured alone or cocultured with
More informationSupplementary Figure 1. IL-12 serum levels and frequency of subsets in FL patients. (A) IL-12
1 Supplementary Data Figure legends Supplementary Figure 1. IL-12 serum levels and frequency of subsets in FL patients. (A) IL-12 serum levels measured by multiplex ELISA (Luminex) in FL patients before
More informationA. Generation and characterization of Ras-expressing autophagycompetent
Supplemental Material Supplemental Figure Legends Fig. S1 A. Generation and characterization of Ras-expressing autophagycompetent and -deficient cell lines. HA-tagged H-ras V12 was stably expressed in
More informationSupplementary Information
Supplementary Information Figure S1. Int6 gene silencing efficiency. (A) Western Blot analysis of Int6 expression at different times after sirna transfection. Int6 expression is strongly silenced in Int6
More information6. TNF-α regulates oxidative stress, mitochondrial function and autophagy in neuronal cells
6. TNF-α regulates oxidative stress, mitochondrial function and autophagy in neuronal cells 6.1 TNF-α induces mitochondrial oxidative stress in SH-SY5Y cells. The dysregulation of mitochondria and oxidative
More informationSupplemental Table I.
Supplemental Table I Male / Mean ± SEM n Mean ± SEM n Body weight, g 29.2±0.4 17 29.7±0.5 17 Total cholesterol, mg/dl 534.0±30.8 17 561.6±26.1 17 HDL-cholesterol, mg/dl 9.6±0.8 17 10.1±0.7 17 Triglycerides,
More informationIn vitro human regulatory T cell suppression assay
Human CD4 + CD25 + regulatory T cell isolation, in vitro suppression assay and analysis In vitro human regulatory T cell suppression assay Introduction Regulatory T (Treg) cells are a subpopulation of
More informationBioactivity Assays: Putting the Puzzle Together
Bioactivity Assays: Putting the Puzzle Together Dr. Ulrike Herbrand Department for Biosafety & Bioassay Services Charles River Biologics Testing Solutions Outline General Remarks MoA reflecting bioassays
More informationCellometer Image Cytometry for Cell Cycle Analysis
Cellometer Cytometry for Cell Cycle Analysis Importance of Cell Cycle Research Oncology: Since cancer cells often undergo abnormal cell division and proliferation, it is important to understand the cell
More informationAssays for Immuno-oncology Research Real-time automated measurements of immune and tumor cell dynamics within your incubator
INCUCYTE LIVE-CELL ANALYSIS SYSTEM Assays for Immuno-oncology Research Real-time automated measurements of immune and tumor cell dynamics within your incubator See what your cells are doing and when they
More informationThird line of Defense. Topic 8 Specific Immunity (adaptive) (18) 3 rd Line = Prophylaxis via Immunization!
Topic 8 Specific Immunity (adaptive) (18) Topics - 3 rd Line of Defense - B cells - T cells - Specific Immunities 1 3 rd Line = Prophylaxis via Immunization! (a) A painting of Edward Jenner depicts a cow
More informationImpact factor: Reporter:4A1H0019 Chen Zi Hao 4A1H0023 Huang Wan ting 4A1H0039 Sue Yi Zhu 4A1H0070 Lin Guan cheng 4A1H0077 Chen Bo xuan
Curcumin Protects Neonatal Rat Cardiomyocytes against High Glucose-Induced Apoptosis via PI3K/Akt Signalling Pathway Wei Yu,1,2 Wenliang Zha,1 Zhiqiang Ke,1 Qing Min,2 Cairong Li,1 Huirong Sun,3 and Chao
More informationIn vitro human regulatory T cell expansion
- 1 - Human CD4 + CD25 + regulatory T cell isolation, Workflow in vitro expansion and analysis In vitro human regulatory T cell expansion Introduction Regulatory T (Treg) cells are a subpopulation of T
More informationCytoPainter Lysosomal Staining Kit - Blue Fluorescence
ab112135 CytoPainter Lysosomal Staining Kit - Blue Fluorescence Instructions for Use For staining Lysosomes in suspension and adherent cells by using our proprietary blue fluorescence probe This product
More informationSupplementary Materials and Methods
Supplementary Materials and Methods Immunoblotting Immunoblot analysis was performed as described previously (1). Due to high-molecular weight of MUC4 (~ 950 kda) and MUC1 (~ 250 kda) proteins, electrophoresis
More informationA dual PI3 kinase/mtor inhibitor reveals emergent efficacy in glioma
Supplemental data A dual PI3 kinase/mtor inhibitor reveals emergent efficacy in glioma Qi-Wen Fan, Zachary A. Knight, David D. Goldenberg, Wei Yu, Keith E. Mostov, David Stokoe, Kevan M. Shokat, and William
More informationCaractérisation et méthodes d études de la mort cellulaire par cytométrie en flux
Caractérisation et méthodes d études de la mort cellulaire par cytométrie en flux Université de Bourgogne Gérard LIZARD - Inserm EA7270 - Equipe Biochimie du Peroxysome, inflammation et Métabolisme Lipidique
More informationBead Based Assays for Cytokine Detection
Bead Based Assays for Cytokine Detection September 27, 2014 6 th EFIS-EJI South East European Immunology School SEEIS 2014 Timisoara, Romania The Cells of the Immune System The Immune Reaction (Th2) (Th1)
More informationSestrin2 and BNIP3 (Bcl-2/adenovirus E1B 19kDa-interacting. protein3) regulate autophagy and mitophagy in renal tubular cells in. acute kidney injury
Sestrin2 and BNIP3 (Bcl-2/adenovirus E1B 19kDa-interacting protein3) regulate autophagy and mitophagy in renal tubular cells in acute kidney injury by Masayuki Ishihara 1, Madoka Urushido 2, Kazu Hamada
More informationGrowth and Differentiation Phosphorylation Sampler Kit
Growth and Differentiation Phosphorylation Sampler Kit E 0 5 1 0 1 4 Kits Includes Cat. Quantity Application Reactivity Source Akt (Phospho-Ser473) E011054-1 50μg/50μl IHC, WB Human, Mouse, Rat Rabbit
More informationA particular set of insults induces apoptosis (part 1), which, if inhibited, can switch to autophagy. At least in some cellular settings, autophagy se
A particular set of insults induces apoptosis (part 1), which, if inhibited, can switch to autophagy. At least in some cellular settings, autophagy serves as a defence mechanism that prevents or retards
More informationProstaglandin E 2 reduces radiation-induced epithelial apoptosis through a mechanism involving AKT activation and bax translocation
Research article Prostaglandin E 2 reduces radiation-induced epithelial apoptosis through a mechanism involving AKT activation and bax translocation Teresa G. Tessner, Filipe Muhale, Terrence E. Riehl,
More informationPhospho-AKT Sampler Kit
Phospho-AKT Sampler Kit E 0 5 1 0 0 3 Kits Includes Cat. Quantity Application Reactivity Source Akt (Ab-473) Antibody E021054-1 50μg/50μl IHC, WB Human, Mouse, Rat Rabbit Akt (Phospho-Ser473) Antibody
More informationab Annexin V- mfluor Blue 570 Detection
Version 1 Last updated 26 March 2018 ab219914 Annexin V- mfluor Blue 570 Detection Reagent For the rapid, sensitive and accurate measurement of PS exposure in live cells This product is for research use
More informationSupplementary Figure 1
Supplementary Figure 1 Expression of apoptosis-related genes in tumor T reg cells. (a) Identification of FOXP3 T reg cells by FACS. CD45 + cells were gated as enriched lymphoid cell populations with low-granularity.
More informationSupplementary Figure 1
Supplementary Figure 1 6 HE-50 HE-116 E-1 HE-108 Supplementary Figure 1. Targeted drug response curves of endometrial cancer cells. Endometrial cancer cell lines were incubated with serial dilutions of
More informationAssays for Immunology Research IncuCyte S3 Live-Cell Analysis System
ssays for Immunology Research IncuCyte S3 Live-Cell nalysis System Immune system characterization of health and disease states requires quantitative data from dynamic experimental models of the cells that
More informationab65311 Cytochrome c Releasing Apoptosis Assay Kit
ab65311 Cytochrome c Releasing Apoptosis Assay Kit Instructions for Use For the rapid, sensitive and accurate detection of Cytochrome c translocation from Mitochondria into Cytosol during Apoptosis in
More informationSUPPLEMENTARY INFORMATION
Supplementary Figures Supplementary Figure S1. Binding of full-length OGT and deletion mutants to PIP strips (Echelon Biosciences). Supplementary Figure S2. Binding of the OGT (919-1036) fragments with
More informationSupplementary Figure 1 Role of Raf-1 in TLR2-Dectin-1-mediated cytokine expression
Supplementary Figure 1 Supplementary Figure 1 Role of Raf-1 in TLR2-Dectin-1-mediated cytokine expression. Quantitative real-time PCR of indicated mrnas in DCs stimulated with TLR2-Dectin-1 agonist zymosan
More informationp47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO
Supplementary Information p47 negatively regulates IKK activation by inducing the lysosomal degradation of polyubiquitinated NEMO Yuri Shibata, Masaaki Oyama, Hiroko Kozuka-Hata, Xiao Han, Yuetsu Tanaka,
More informationSupplementary Figures
Supplementary Figures Supplementary Fig. 1. Surface thiol groups and reduction of activated T cells. (a) Activated CD8 + T-cells have high expression levels of free thiol groups on cell surface proteins.
More informationQuestion 1. Kupffer cells, microglial cells and osteoclasts are all examples of what type of immune system cell?
Abbas Chapter 2: Sarah Spriet February 8, 2015 Question 1. Kupffer cells, microglial cells and osteoclasts are all examples of what type of immune system cell? a. Dendritic cells b. Macrophages c. Monocytes
More informationAnnexin V-Cy3 Apoptosis Detection Reagent
ab14143 Annexin V-Cy3 Apoptosis Detection Reagent Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in various samples This product is for research use only and is not
More informationDURACLONE IF BE CERTAIN ABOUT THE RESPONSE. l res. a il n c n. For Research Use Only - Not for use in Diagnostic procedures
DURACLONE IF earch tria l res lc a om ic il n c n nio pa Yo ur BE CERTAIN ABOUT THE RESPONSE For Research Use Only - Not for use in Diagnostic procedures BE CERTAIN ABOUT THE RESPONSE The sensitive and
More informationhexahistidine tagged GRP78 devoid of the KDEL motif (GRP78-His) on SDS-PAGE. This
SUPPLEMENTAL FIGURE LEGEND Fig. S1. Generation and characterization of. (A) Coomassie staining of soluble hexahistidine tagged GRP78 devoid of the KDEL motif (GRP78-His) on SDS-PAGE. This protein was expressed
More informationRayBio KinaseSTAR TM Akt Activity Assay Kit
Activity Assay Kit User Manual Version 1.0 March 13, 2015 RayBio KinaseSTAR TM Akt Activity Kit Protocol (Cat#: 68AT-Akt-S40) RayBiotech, Inc. We Provide You With Excellent Support And Service Tel:(Toll
More informationNFκB What is it and What s the deal with radicals?
The Virtual Free Radical School NFκB What is it and What s the deal with radicals? Emily Ho, Ph.D Linus Pauling Institute Scientist Department of Nutrition and Food Management Oregon State University 117
More informationSupplementary material. Supplementary Figure legends
Supplementary material Supplementary Figure legends Supplementary Figure 1: Senescence-associated proliferation stop in response to oncogenic N-RAS expression Proliferation of NHEM cells without (ctrl.)
More informationLecture 36: Review of membrane function
Chem*3560 Lecture 36: Review of membrane function Membrane: Lipid bilayer with embedded or associated proteins. Bilayers: 40-70% neutral phospholipid 10-20% negative phospholipid 10-30% cholesterol 10-30%
More informationSerafino et al. Thymosin α1 activates complement receptor-mediated phagocytosis in human monocyte-derived macrophages. SUPPLEMENTARY FIGURES
Supplementary Fig. S1. Evaluation of the purity and maturation of macrophage cultures tested by flow cytometry. The lymphocytic/monocytic cellular fraction was isolated from buffy coats of healthy donors
More informationDuraClone IM. Standardized phenotyping panels for studies of the human immune system
DuraClone IM Standardized phenotyping panels for studies of the human immune system Standardize with the experts, adopt DuraClone IM Tubes. Clinical research studies require accurate, reproducible results
More information1. endoplasmic reticulum This is the location where N-linked oligosaccharide is initially synthesized and attached to glycoproteins.
Biology 4410 Name Spring 2006 Exam 2 A. Multiple Choice, 2 pt each Pick the best choice from the list of choices, and write it in the space provided. Some choices may be used more than once, and other
More informationSUPPLEMENT. Materials and methods
SUPPLEMENT Materials and methods Cell culture and reagents Cell media and reagents were from Invitrogen unless otherwise indicated. Antibiotics and Tet-certified serum were from Clontech. In experiments
More informationAndrea s Final Exam Review PCB 3233 Spring Practice Final Exam
NOTE: Practice Final Exam Although I am posting the answer key for this practice exam, I want you to use this practice to gauge your knowledge, and try to figure out the right answer by yourself before
More informationPhagocytosis of FITC labelled opsonized and non-opsonized E. coli bacteria by monocytes and granulocytes in a whole blood assay
Phagocytosis of FITC labelled opsonized and non-opsonized E. coli bacteria by monocytes and granulocytes in a whole blood assay APPLICATION NOTE Author: Andreas Spittler, MD, Associate Professor for Pathophysiology
More informationAperTO - Archivio Istituzionale Open Access dell'università di Torino
AperTO - Archivio Istituzionale Open Access dell'università di Torino From the nucleus to the mitochondria and backthe odyssey of a multitask STAT3 This is the author's manuscript Original Citation: From
More informationSupplementary Table e-1. Flow cytometry reagents and staining combinations
Supplementary data Supplementary Table e-1. Flow cytometry reagents and staining combinations Reagents Antibody Fluorochrome Clone Source conjugation CD3 FITC UCHT1 BD Biosciences CD3 PerCP-Cy5.5 SK7 Biolegend
More informationElectron Transport Chain and Oxidative phosphorylation
Electron Transport Chain and Oxidative phosphorylation So far we have discussed the catabolism involving oxidation of 6 carbons of glucose to CO 2 via glycolysis and CAC without any oxygen molecule directly
More informationC-Phycocyanin (C-PC) is a n«sjfc&c- waefc-jduble phycobiliprotein. pigment isolated from Spirulina platensis. This water- soluble protein pigment is
' ^Summary C-Phycocyanin (C-PC) is a n«sjfc&c- waefc-jduble phycobiliprotein pigment isolated from Spirulina platensis. This water- soluble protein pigment is of greater importance because of its various
More informationSupplementary Figure Legends
Supplementary Figure Legends Supplementary Figure 1. Comparison of RNP IC-mediated NET formation. Quantification of DNA release induced by ICs consisting of SmRNP combined with SLE IgG 961 (n = 10), 1032
More informationAnnexin V-Cy3 Apoptosis Detection Kit
ab14142 Annexin V-Cy3 Apoptosis Detection Kit Instructions for Use For the rapid, sensitive and accurate measurement of apoptosis in various samples. This product is for research use only and is not intended
More informationAnalysis of Sphingosine Kinase Activity in Single Natural Killer Cells from Peripheral Blood
Electronic Supplementary Material (ESI) for Integrative Biology. This journal is The Royal Society of Chemistry 2015 Electronic Supplementary Information: Analysis of Sphingosine Kinase Activity in Single
More informationAnnexin V APC Assay Kit
Annexin V APC Assay Kit Item No. 601410 www.caymanchem.com Customer Service 800.364.9897 Technical Support 888.526.5351 1180 E. Ellsworth Rd Ann Arbor, MI USA TABLE OF CONTENTS GENERAL INFORMATION 3 Materials
More informationCytoPainter LysoGreen Indicator Reagent
ab176826 CytoPainter LysoGreen Indicator Reagent Instructions for Use For staining lysosomes in live cells with our proprietary Green probe. This product is for research use only and is not intended for
More informationApplication Information Bulletin: Cell Signaling
Application Information Bulletin: Cell Signaling Detection of Both Surface Proteins and Intracellular Phospho-Determinants in Whole Blood Using the PerFix-p Reagent System William L. Godfrey 1, Li Yang
More informationTSH Receptor Monoclonal Antibody (49) Catalog Number MA3-218 Product data sheet
Website: thermofisher.com Customer Service (US): 1 800 955 6288 ext. 1 Technical Support (US): 1 800 955 6288 ext. 441 TSH Receptor Monoclonal Antibody (49) Catalog Number MA3-218 Product data sheet Details
More informationCell isolation. Spleen and lymph nodes (axillary, inguinal) were removed from mice
Supplementary Methods: Cell isolation. Spleen and lymph nodes (axillary, inguinal) were removed from mice and gently meshed in DMEM containing 10% FBS to prepare for single cell suspensions. CD4 + CD25
More informationSignaling. Dr. Sujata Persad Katz Group Centre for Pharmacy & Health research
Signaling Dr. Sujata Persad 3-020 Katz Group Centre for Pharmacy & Health research E-mail:sujata.persad@ualberta.ca 1 Growth Factor Receptors and Other Signaling Pathways What we will cover today: How
More information