IMMUNOLOGY. Source, Isolate, Culture, And Analyze Immune Cells. Scientists Helping Scientists

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1 IMMUNOLOGY Source, Isolate, Culture, And Analyze Immune Cells Scientists Helping Scientists

2 TABLE OF CONTENTS Tools For Your Immunology Research 4 Primary Cells: It All Starts with The Right Cells 5 Cell Isolation: Highly Purified Cells for Any Downstream Application 6 EasySep : Fast and Easy Immunomagnetic Cell Isolation 7 EasySep for Mouse Cells 8 EasySep for Human Cells 1 RoboSep : Fully Automated Immunomagnetic Cell Isolation 12 SepMate : Hassle-Free Isolation 13 RosetteSep : Unique Immunodensity Cell Isolation 14 Specialized Media and Supplements 15 High-Quality, Reliable Antibodies Product Listing 16 Human Primary Cells 18 Human Cell Isolation Products 18 T Cells & Subsets 22 B Cells & Subsets 23 NK Cells 24 Other Lymphocytes & Subsets 25 Dendritic Cells 25 Monocytes 25 Granulocytes & Subsets 26 Myeloid Cells 27 Hematopoietic Progenitors 28 Other Cell Types 29 Human Cell Depletion Products 3 Mouse Cell Isolation Products 3 T Cells & Subsets 31 B Cells & Subsets 31 NK Cells 31 Other Lymphocytes & Subsets 32 Dendritic Cells 32 Monocytes 32 Neutrophils 33 Myeloid Cells 33 Hematopoietic Progenitors 34 Other Cell Types 35 Other Species Cell Isolation Products 36 Cell Isolation Magnets and Accessories 37 Instruments 38 Specialized Cell Culture Media, Supplements and Cryopresevation Media 39 Cytokines and ELISA Kits

3 Tools For Your Immunology Research From primary human cells to cell isolation kits, culture media, and antibodies, STEMCELL Technologies provides the tools you need for every step of your immunology research. STEMCELL Products For Every Step of Your Immune Cell-Based Research Sourcing Isolation Activation, Expansion, & Differentiation Analysis Fresh human cells EasySep ImmunoCult Antibodies Cryopreserved human cells RoboSep Cytokines ELISA kits RosetteSep CryoStor SepMate Lymphoprep 3

4 IMMUNOLOGY RESEARCH PRODUCTS Primary Cells It All Starts with the Right Cells Sourcing Isolation Activation, Expansion, & Differentiation Analysis Starting with the right primary cells as the foundation for your experiments is the first step towards success in your research. Choose from a wide range of fresh or cryopreserved human primary cells isolated from peripheral blood, cord blood, bone marrow, and mobilized peripheral blood. 1,2 Cryopreserved immune and progenitor cells are isolated from full-size Leuko Paks (leukapheresis preparations) or entire umbilical cords and are ready to use upon receipt. For users requiring fresh unprocessed tissue samples, whole peripheral blood, whole bone marrow, and Leuko Paks are also available. Cells are shipped with a Certificate of Analysis indicating guaranteed Quality Control testing results including cell count, viability, and purity. All human primary cell products are ethically sourced using informed consent forms (ICFs) and protocols approved by either the Food and Drug Administration (FDA) or an Institutional Review Board (IRB), ensuring the protection of personal information and donor anonymity. 3 Why Use STEMCELL's Primary Cells? PHYSIOLOGICAL RELEVANCE. Choose cells that are physiologically representative of cells in vivo. ACCESS. Obtain large lots of cryopreserved cells, including cells that are rare or difficult to isolate, reducing inter-assay variability. CONVENIENCE. Start your experiments according to your schedule, without being restricted by the availability of tissue samples. FLEXIBLE HOLD POLICIES. Reserve large numbers of cryopreserved cells while you test in your specific applications. See pages 16 and 17 for a complete listing of mononuclear cells, isolated subsets, plasma, and unprocessed tissues from normal and diseased donors, or visit Figure 1. Fresh Human Peripheral Blood Leuko Pak (Catalog #75) Leuko Pak containing peripheral blood mononuclear cells (s) enriched using the Spectra Optia Apheresis System from a normal donor. Figure 2. Human T Cells Isolated from Leukapheresis (Leuko Pak) Preparations T cells isolated using the EasySep Human T Cell (Catalog #17951), stimulated with ImmunoCult Human CD3/CD28 T Cell Activator (Catalog #1971) and cultured in ImmunoCult -XF T Cell Expansion Medium (Catalog #1981). 1. Certain cryopreserved products are only available in select territories. Please contact Product and Scientific Support (techsupport@stemcell.com) for further information. 2. Fresh products currently available in the United States and Canada (excluding Quebec). Please contact Product and Scientific Support (techsupport@stemcell.com) for further information. 3. Leuko Pak,, and Bone Marrow (LP, WB, and BM) Donor Screening: Donors are screened for HIV (1 & 2), Hepatitis B, and Hepatitis C. Cryopreserved LP, WB, and BM: Products are shipped with negative test results from donor screening that is done within 9 days of collection. Fresh LP, WB, and BM: If the donor has been screened within 9 days of donation the product will be shipped with negative test results from donor screening. If the donor has not been screened within 9 days of collection, a test sample will be taken at the time of donation and the product will be shipped before the screening results are available. In the unlikely event that a test result is positive, the customer will be contacted as soon as possible (usually within 2-4 business days from the time of shipment). Cord Blood (CB) Donor Screening: Testing for HIV-1 and 2, Hepatitis B, and Hepatitis C is performed on a maternal blood sample and/or on a sample of the donated cord blood. Cryopreserved CB: Products are shipped with negative test results from donor screening. 4 STEMCELL Technologies Inc.

5 Cell Isolation Highly Purified Cells for Any Downstream Application Sourcing Isolation Activation, Expansion, & Differentiation Analysis Ensure your isolated cells are viable and suitable for downstream functional and biological studies using STEMCELL Technologies fast, easy, and column-free cell separation technologies. Isolate highly purified cells from virtually any sample source, including peripheral blood mononuculear cells, spleen, whole blood, bone marrow, and leukapheresis (i.e., Leuko Pak) samples. EasySep TM Fast and Easy Immunomagnetic Cell Isolation EasySep isolates cells quickly and easily without the use of columns in as little as 8 minutes. With a simple pour, isolated cells are immediately ready for downstream use. RoboSep TM Fully Automated Immunomagnetic Cell Isolation RoboSep -S and RoboSep -16 fully automate all cell labeling and separation steps of the EasySep procedure, minimizing sample handling and freeing up technician time. SepMate TM Hassle-Free Isolation SepMate simplifies mononuclear cell isolation during density gradient centrifugation by eliminating the need to carefully layer samples or pipette off isolated cells. SepMate is suitable for In Vitro Diagnostic (IVD) applications. 1 RosetteSep TM Unique Immunodensity Cell Isolation RosetteSep isolates highly purified cells directly from human whole blood during density gradient centrifugation, reducing your cell isolation workflow to a single step. 1. SepMate IVD is registered as an In Vitro Diagnostic (IVD) device intended for the isolation of mononuclear cells from human whole blood or cord blood by density gradient centrifugation in Canada, the United States, Europe, and Australia. In all other regions, SepMate RUO is available for Research Use Only. 5

6 IMMUNOLOGY RESEARCH PRODUCTS EasySep TM Fast and Easy Immunomagnetic Cell Isolation EasySep is a powerful immunomagnetic cell isolation platform that combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system for fast and easy isolation of highly purified cell populations that are immediately ready for downstream applications. Cells are targeted for either depletion (negative selection) or selection (positive selection) using antibody complexes directed to specific cell surface antigens. The antibody complexes link targeted cells to magnetic particles. Labeled cells are pulled to the sides of the tube when the sample is placed in an EasySep magnet. Magnetically labeled cells will remain in the tube while the untouched cells can be simply poured or pipetted off into a new tube. Why Use EasySep? FAST AND EASY. Isolate cells from virtually any sample source in as little as 8 minutes without columns or washes. GENTLE. Obtain viable and functional cells that are immediately ready for downstream applications. HIGH PURITY. Achieve up to 99% cell purity with high recovery. VIDEO Fast and Easy Cell Isolation with EasySep EasySep Magnets THE ICONIC COLUMN- FREE CELL ISOLATION PLATFORM FOR LARGER COLUMN- FREE CELL ISOLATIONS FOR VERY LARGE VOLUME CELL ISOLATIONS FOR SIMULTANEOUS OF MULTIPLE SAMPLES FOR SIMULTANEOUS SMALL VOLUME CELL ISOLATIONS EasySep Magnet 1 "The Big Easy" EasySep Magnet 2 Easy 5 EasySep Magnet EasyEights EasySep Magnet EasyPlate EasySep Magnet Catalog # Number of Samples on each side = 16 total 96 Start Sample Cell Number Range x 1 8 cells per 5 ml tube.2-1 x 1 8 cells per 14 ml tube.5-2 x 1 8 cells per 5 ml tube x 1 8 cells per 5 ml tube x 1 8 cells per 14 ml tube x 1 8 cells per well Collection Method Pour off Pour off Pipette off Pipette off Pipette off 1. Multiple EasySep magnets can be used together with the EasySep Multistand (Catalog #181) for processing up to 4 samples simultaneously or with up to 6 EasySep EasyStands (Catalog #1813) for processing up to 6 samples (see page 36). 2. Multiple The Big Easy EasySep magnets can be used together with the EasySep Multistand (Catalog #181) for processing up to 4 samples simultaneously (see page 36). 6 STEMCELL Technologies Inc.

7 EasySep for Mouse Cells TM Immunomagnetic Cell Isolation in as Little as 15 Minutes EasySep kits are available for the isolation of mouse cells in as little as 15 minutes from a variety of sample sources, including spleen, bone marrow, lymph nodes, and whole blood. Our EasySep negative selection kits use biotinylated antibodies to target unwanted cells, while positive selection kits use an antibody complex to select cells of interest. Typical EasySep Mouse Cell Isolation Protocol (Negative Selection) 1 2 Add EasySep Isolation Cocktail to single-cell suspension. 4 3 Add EasySep RapidSpheres to cell suspension. Place tube in EasySep Magnet and incubate for 2.5 minutes*. Pour off desired fraction into a new tube. 3 Incubate 1 minutes* Incubate 2.5 minutes* 4 Pipette off desired fractions into new tubes. Place tubes in EasyEights EasySep Magnet and incubate for 5 minutes*. *Times are typical for next-generation negative selection kits. Time for each kit will vary depending on the exact isolation protocol and magnet used. CD4 FITC CD4 FITC Start Isolated CD11c PE Isolated CD11c PE Start MHC II FITC MHC II FITC Figure 3. EasySep Mouse CD4+ T Cell (Catalog #19852) Figure 4. EasySep Mouse CD11c Positive Selection Kit II (Catalog #1878) Starting with mouse splenocytes, the CD4+ T cell content of the isolated fraction typically ranges from 89-96% (gated on viable singlet cells). In the example above, the purities of the start and isolated fractions are 24.6% and 95.7%, respectively. Starting with mouse splenocytes, the CD11c+ cell content of the isolated fraction typically ranges from 86.8 ± 9.7% (gated on viable singlet cells). In the example above, the purities of the start and isolated fractions are 5.7% and 92.3%, respectively. 7

8 IMMUNOLOGY RESEARCH PRODUCTS EasySep TM for Human Cells Immunomagnetic Cell Isolation in as Little as 8 Minutes EasySep kits are available for the isolation of human cells from a variety of sample sources, including peripheral blood mononuclear cells (s), whole blood, leukapheresis (i.e., Leuko Pak) samples, bone marrow, and cord blood. Cells of interest are targeted with antibody complexes and magnetic particles for negative or positive selection. Isolate highly purified, untouched, functional human immune cells in as little as 8 minutes with our next-generation EasySep cell isolation kits. Did You Know? There are more cell isolation options than ever with EasySep. EASYSEP DIRECT. Isolate cells straight from whole blood without the need for lysis or centrifugation. EASYSEP RELEASE. Isolate unique and rare cell types by quickly and easily removing magnetic particles from positively selected cells. Typical EasySep Human Cell Isolation Protocol (Negative Selection) Add EasySep Isolation Cocktail to single-cell suspension. Add EasySep RapidSpheres to cell suspension. Place tube in EasySep Magnet and incubate for 3 minutes*. Pour off desired fraction into a new tube. Incubate 5 minutes* Incubate minutes* *Times are typical for next-generation negative selection kits. Time for each kit will vary depending on the exact isolation protocol and magnet used. No particle incubation step is required for next-generation negative selection protocols. Start Isolated Start Isolated START START ISOLATED ISOLATED CD4 APC CD4 APC Count (x1) Count (x1) Count (x1) Count (x1) CD3 PerCP-Cy5.5 CD3 PerCP-Cy5.5 CD138 BV CD138 CD138 PE PE CD138 BV CD138 CD138 PE PE Figure 5. EasySep Human CD4 + T Cell (Catalog #17952) Starting with human peripheral blood mononuclear cells, the CD4 + T cell (CD3 + CD4 + ) content of the isolated fraction is typically 94.8 ± 2.3% (mean ± SD; gated on viable singlet cells). In the example above, the purities of the start and isolated fractions are 39.4% and 98.2%, respectively. Figure 6. EasySep Human and Bone Marrow CD138 Positive Selection Kit II (Catalog #17887) Starting with fresh whole blood spiked with a multiple myeloma cell line, U266, the CD138+ cell content of the selected fraction typically ranges from %. In the above example, the purities of the start and final isolated fractions are 9.1% and 9.4%, respectively. 8 STEMCELL Technologies Inc.

9 Viable and Functional Cells for Your Immunology Research A 8 CD25 Expression (Day ) B 8 CD25 Expression (Day 3) No Stimulation Anti-CD3/CD28 1 Start % CD25+ cells % CD25+ cells % 7AAD - CD Pan-T Cells CD4+ T Cells NK Cells Monocytes Neutrophils Negative Selection Positive Selection C % CD69+ cells CD3 (Pos Sel) Pan-T Cell (Neg Sel) CD69 Expression (Day ) CD3 (Pos Sel) Pan-T Cell (Neg Sel) D % CD69+ cells CD3 (Pos Sel) CD69 Expression (Day 3) 8 No Stimulation Anti-CD3/CD CD3 (Pos Sel) Pan-T Cell (Neg Sel) Pan-T Cell (Neg Sel) Figure 7. Immune Cells Isolated Using EasySep Technology Are Highly Viable Immune cells were isolated from processed leukapheresis or peripheral blood samples using EasySep positive selection or negative selection kits. Pre- and post-isolation samples were stained with the cell viability dye 7-AAD and appropriate cell surface markers. Cells isolated using EasySep show no significant decrease in viability compared to the starting samples. Data shown as mean ± SEM. Start Figure 8. Human T cells Isolated Using EasySep Show Appropriate Activation Status T cells isolated from s using EasySep positive selection ("Pos Sel") or negative selection ("Neg Sel") kits were assessed for CD25 and CD69 expression immediately after isolation (Day ) and after 3 days in culture with or without CD3/CD28 stimulation. (A,C) At Day, isolated T cells express similar levels of CD25 and CD69 compared to unmanipulated CD3 + s. (B,D) At Day 3, cells remain unactivated in the absence of stimulation, and upregulate activation markers CD25 and CD69 when stimulated. Data shown as mean ± SEM. Figure 9. Human Monocytes Isolated Using EasySep Differentiate and Mature Appropriately upon Stimulation Day 7 immature DC Day 7 mature DC Human monocytes were isolated using EasySep positive selection or negative selection kits and then cultured and differentiated into mature dendritic cells (DCs). At Day 5, cells were cultured with maturation supplement for 2 days (mature DCs) or without maturation supplement (immature DCs). As a control, on Day 5 a whole leukapheresis sample was plated and adherence selected for 2 hours. Non-adherent cells were washed away and adherent cells were cultured with maturation supplement for 2 days. The expression of CD8, CD83, and CD86 in immature and mature DCs was determined by flow cytometry. At Day 7, cells express the mature DC markers CD8, CD83, and CD CD8 CD83 CD86 Leukapheresis CD14 Positive Selection Monocyte Negative Selection 9

10 IMMUNOLOGY RESEARCH PRODUCTS RoboSep TM Fully Automated Immunomagnetic Cell Isolation RoboSep instruments offer true walk-away automation of immunomagnetic cell separation. Using EasySep reagents, RoboSep -S and RoboSep -16 perform all cell labeling and magnetic isolation steps for up to four and sixteen samples, respectively. Sample handling is minimized and the use of disposable tips in these column-free systems ensures that isolated cells of interest are immediately available for any downstream application. RoboSep TM -S RoboSep TM -16 The compact design of RoboSep -S brings the convenience of automated cell isolation to any busy laboratory. The enhanced liquid-handling capabilities of RoboSep -16 allow high-volume users to efficiently isolate desired cells with speed and confidence. Why Use RoboSep? FULLY AUTOMATED. Just load reagents and samples and walk away. SIMULTANEOUS OR SEQUENTIAL CELL ISOLATION. Perform simultaneous cell isolations for up to 4 samples using RoboSep -S and 16 samples using RoboSep -16, or sequentially isolate different cell types from the same sample. NO CROSS-CONTAMINATION. Cells are handled with single-use disposable tips, never passing through reusable columns or tubing. VERSATILE. Use EasySep technology to isolate virtually any cell type from any species or sample, including whole blood. Programs are cell type-specific and customizable. 1 STEMCELL Technologies Inc.

11 How RoboSep Works RoboSep -S and RoboSep -16 fit easily into the workflow of any lab that needs the multi-sample processing capacity, speed, reliability, and convenience of automated cell isolation. Start your cell isolation protocol with as little as 5 minutes of hands-on time with RoboSep -S and RoboSep -16. Both pre-programmed routine protocols and customizable protocols are available to meet your unique cell isolation needs. Typical RoboSep -S Protocol 1 Select protocol. Load sample, 2 Press Run. 3 EasySep reagents, buffer, and tips in carousel. Return in 25 to 6 minutes to collect your separated cells. Typical RoboSep -16 Protocol 1 Select protocol. Load sample, 2 Press Run. 3 EasySep reagents, buffer, and tips. Return in 25 to 6 minutes to collect your separated cells. We like the reliability of the RoboSep, the minimization/elimination of specimen handling by the tech during subset separation, and the low maintenance of the instrument. These factors are important to us with such a high throughput of samples processed. PRODUCT TOUR See RoboSep Instruments in Action Wendy Leong, Senior Clinical Laboratory Scientist PATHOLOGY/STANFORD BLOOD CENTER LABORATORY 11

12 4 5 IMMUNOLOGY RESEARCH PRODUCTS SepMate TM Hassle-Free Isolation SepMate is a specialized tube for fast and easy isolation in just 15 minutes. The SepMate tube contains a unique insert that prevents the density gradient medium 1 (e.g., Ficoll-Paque or Lymphoprep ) and blood sample from mixing. The density gradient medium is pipetted through a central hole in the insert, and the sample is poured or rapidly pipetted on top of the insert. This eliminates the need to carefully layer the sample directly onto the density gradient medium, an otherwise time-consuming and highly laborious step. Only 1 minutes of centrifugation are required, and this step can be carried out with the brake on, further reducing the total time necessary for separation. After centrifugation, plasma and s are simply poured into a new tube. SepMate is registered as an In Vitro Diagnostic (IVD) device in select regions. 1,2 Why Use SepMate? EASY. Avoid the need for slow and laborious sample layering over the density gradient medium. FAST. Isolate s directly from whole blood in just 15 minutes. CONSISTENT. Eliminate errors and minimize variability between separations. VERSATILE. Combine with RosetteSep to isolate purified cell subsets from whole blood in 25 minutes. 3 REGISTERED. Use with whole blood or bone marrow samples for In Vitro Diagnostic (IVD) applications. 1 Typical SepMate Protocol 1 Add density gradient medium through SepMate insert. SepMate insert Density gradient medium (e.g. Ficoll-Paque or Lymphoprep ) Products 2 Pipette sample onto insert. SepMate insert PRODUCT NAME SepMate -15 (IVD) SepMate -15 (RUO) SepMate -5 (IVD) SepMate -5 (RUO) BLOOD VOLUME PROCESSED (ML) UNIT SIZE 1 tubes 3 Centrifuge for 1 minutes (brake on). PRODUCT NAME DENSITY UNIT SIZE 4 Lymphoprep g/ml 5 ml Plasma s Density gradient medium RBCs and granulocytes Pour s 1. SepMate (IVD) is available only in Canada, the United States, Europe, and Australia, where it is registered as an In Vitro Diagnostic (IVD) device for the isolation of mononuclear cells from human whole blood or bone marrow by density gradient centrifugation. 2. SepMate for Research Use Only (RUO) is available in regions where SepMate is not registered as an IVD device. 3. Select RosetteSep products carry the CE mark and are suitable for IVD applications in countries where the CE marking is recognized. Did You Know? Lymphoprep has the same density as Ficoll-Paque, is more cost-effective, and can be substituted for Ficoll-Paque without any need to change your existing protocols. 12 STEMCELL Technologies Inc.

13 RosetteSep TM Unique Immunodensity Cell Isolation RosetteSep is a fast and easy immunodensity procedure for the isolation of untouched cells directly from whole blood. By crosslinking unwanted cells to red blood cells (RBCs) present in the sample, RosetteSep eliminates the need for a separate magnetic separation step because cells are purified during standard density gradient centrifugation. This approach significantly reduces sample handling time and maximizes convenience. Typical RosetteSep Protocol Add RosetteSep Enrichment Cocktail. Layer over density gradient medium. Centrifuge for 2 minutes*. Collect cells. Incubate 2 minutes Plasma Enriched cells Whole blood Add Lymphoprep Density gradient medium Lymphoprep density gradient medium Red blood cells and unwanted cells (rosetted) Highly purified cells are left untouched *Use SepMate to reduce centrifugation time to 1 minutes with brake on. Start: CD45 + Gate Isolated: CD45 + Gate Cell to enrich Red Blood Cells Rosetted (unwanted) cell Magnification 4X CD3 PerCP-Cy5.5 CD56 PE CD3 PerCP-Cy5.5 CD56 PE Figure 1. Micrograph of a Blood Sample After Addition of the RosetteSep Cocktail, and Prior to Density Gradient Centrifugation Figure 11. RosetteSep Human NK Cell Enrichment Cocktail (Catalog #1525) Starting with whole peripheral blood, the NK cell content (gated on CD45 + cells) of the isolated fraction typically ranges from 8-98%. In the example above, the purities of the start and isolated fractions are 7.2% and 92.4%, respectively. RosetteSep TM and SepMate TM Simplified and Standardized Cell Isolation RosetteSep is easily combined with SepMate to rapidly and reproducibly isolate subsets from whole blood. By using the unique SepMate tube, sample throughput is increased and errors associated with improper sample layering are eliminated. This allows even users with minimal training to consistently perform cell isolation by density gradient centrifugation in a busy laboratory environment. 13

14 IMMUNOLOGY RESEARCH PRODUCTS Specialized Media and Supplements For Cell Culture, Activation, Expansion and Differentiation Sourcing Isolation Activation, Expansion, & Differentiation Analysis With high-quality and viable cells as the foundation for your research, integrating STEMCELL Technologies cell culture reagents into your workflow will ensure reliable results. The new ImmunoCult products have been designed to ensure optimal activation, expansion, differentiation, and maintenance of immune cells. These specialized media, activators, and supplements allow you to culture immune cells under defined stimulatory conditions to activate, expand, or differentiate the cell population of interest. For a detailed list of ImmunoCult products, see page 38 or visit ImmunoCult for T Cell Research Advance your basic T cell immunology and clinical T cell engineering research with ImmunoCult T cell activators, expansion medium, and differentiation supplements designed for: Robust human T cell activation and expansion without the use of magnetic beads, feeder cells, or antigens. Consistent human T cell expansion in serum- and xeno-free expansion medium. Differentiation of human and mouse T cells into Th1, Th2, and regulatory T cell subsets. ImmunoCult for Dendritic Cell Research Dendritic cells (DCs) are potent antigen-presenting cells and key regulators of the immune response. These cells are of great interest in vaccine development research for cancer and infectious diseases. Further your immunology research with ImmunoCult -ACF Dendritic Cell Medium and Supplements designed for: Generation of mature DCs from isolated monocytes. Obtaining a high yield of mature DCs with the desired phenotype and function. ImmunoCult for Macrophage Research Macrophages are key players in the immune response due to their role in immune regulation, tissue repair, and tumor biology. Bring consistency to your macrophage research with ImmunoCult -SF Macrophage Medium designed for: Generation of mature macrophages from isolated monocytes in a serum-free culture. Reliable differentiation and activation of M1 or M2a macrophages using optimized protocols. Activate, expand, or differentiate with cytokines, chemokines, and growth factors from STEMCELL Technologies. These highquality reagents ensure reproducibility across a variety of applications for immunology and stem cell research. To learn more about STEMCELL s cytokines, see page 39 or visit 14 STEMCELL Technologies Inc.

15 High-Quality, Reliable Antibodies Take the Guesswork out of Finding the Right Antibody Sourcing Isolation Activation, Expansion, & Differentiation Analysis Using the right antibody is an essential component for your research. Antibodies from different vendors provide variable and potentially sub-optimal staining profiles. STEMCELL Technologies offers a line of high-quality primary and secondary antibodies that have been verified to work with our cell isolation and cell culture reagents in specific applications, ensuring that your downstream cell analysis, including phenotyping and purity assessments, works consistently. New antibodies are added to our growing inventory on a continuing basis, and Pacific Blue conjugates for some of our most popular antibodies are now available. To find staining antibodies compatible with our cell isolation kits, please refer to pages For a complete list of available antibodies visit Importance of Using a STEMCELL-Verified Antibody Vendor 1 Start Vendor 2 Vendor 3 Isotype Control USING AN ANTIBODY NOT VERIFIED BY STEMCELL CAN LEAD TO INACCURATE RESULTS. Isolated Isotype Control EXAMPLE OF AN ANTIBODY VERIFIED TO WORK WITH OUR CELL ISOLATION KITS. CD24 PE CD4 Alexa Fluor 488 Figure 12. Anti-Mouse CD24 Antibodies (Clone M1/69) from Three Different Vendors Exhibit Varying Staining Profiles Surface expression of CD24 on C57BL/6 mouse bone marrow cells was examined by flow cytometry using anti-mouse CD24, clone M1/69, PE antibody from three different vendors according to their recommended protocols (grey shaded histograms) or Rat IgG2b, kappa PE isotype control antibody (black filled histogram). Vendor 1 corresponds to antibody from STEMCELL Technologies (Catalog #699PE). Figure 13. STEMCELL s Verified Anti-Human CD4, Clone OKT4, Alexa Fluor 488 (Catalog #616AD) Has Been Verified for Purity Assessments of Cells Isolated with EasySep Kits Flow cytometry analysis of human s isolated with the EasySep Human CD4 Positive Selection Kit and labeled with Anti-Human CD4, Clone OKT4, Alexa Fluor 488. Histograms show labeling of the total s (Start) and isolated cells (Isolated). Labeling with a mouse IgG2b, kappa Alexa Fluor 488 isotype control antibody is shown in the bottom panel (open histogram). Why Use STEMCELL's Antibodies? LARGE PORTFOLIO. Large selection of primary and secondary antibodies and isotype controls. COMPATIBLE. Verified to work with our cell isolation and cell culture reagents for select applications. VERSATILE. Proven performance for multiple applications. RELIABLE. Trusted by our dedicated team of research scientists. 15

16 IMMUNOLOGY RESEARCH PRODUCTS Product Listing Human Primary Cells Cryopreserved Human Peripheral Blood Products 1 Fresh Human Peripheral Blood Products 3 DESCRIPTION QUANTITY Peripheral Blood Mononuclear Cells 2 CD34 + Cells Pan-T Cells 15 million cells million cells million cells million cells million cells 74.5 million cells million cells million cells million cells CD4 + T Cells 15 million cells 726 CD4 + CD25 + T Cells 2 million cells 728 CD4 + CD45RA + T Cells 5 million cells 729 CD4 + CD45RO + T Cells 5 million cells 731 CD8 + T Cells 1 million cells 727 CD8 + CD45RA + T Cells 5 million cells 73 B Cells 1 million cells million cells CD19 + CD27 - Naïve B Cells 1 million cells 732 CD19 + B Cells 1 million cells 733 Monocytes 1 million cells 734 CD14 + Monocytes 1 million cells million cells million cells 735 NK Cells 5 million cells 736 DESCRIPTION ANTICOAGULANT 3 QUANTITY Peripheral Blood Leuko Pak 4 ACDA 5 Whole Peripheral Blood ACDA 5 Quarter Size 75.2 Half Size 75.1 Full Size 75 1 x 1 ml x 1 ml x 1 ml x 1 ml x 1 ml x 1 ml ml 754 CP2D 5 45 ml 751 EDTA 5 Na Heparin 5 1 x 1 ml x 1 ml x 1 ml x 1 ml x 1 ml x 1 ml x 1 ml x 1 ml x 1 ml x 1 ml x 1 ml x 1 ml CD56 + NK Cells 5 million cells 737 Macrophages 1.5 million cells 742 BDCA4 + Cells.5 million cells 738 Immature Dendritic Cells 1.5 million cells 741 Plasmacytoid Dendritic Cells.5 million cells ml ml ml Plasma 4 ml ml ml ml For a complete listing of primary cells products, including mobilized peripheral blood products and cultured cells, please visit 16 STEMCELL Technologies Inc.

17 Cryopreserved Diseased State Human Peripheral Blood Mononuclear Cell Products 1,6 DESCRIPTION QUANTITY Rheumatoid Arthritis 1 million cells 75 Cryopreserved Human Umbilical Cord Blood Products 1 DESCRIPTION QUANTITY 15 million cells 77.1 Ulcerative Colitis 1 million cells 751 Crohn s Disease 1 million cells 752 COPD 1 million cells 753 Lupus (SLE) 1 million cells 754 Osteoarthritis 1 million cells 755 Psoriasis 1 million cells 756 Asthma 1 million cells 757 Celiac Disease 1 million cells 758 Diabetes, Type I 1 million cells 761 Mononuclear Cells (MNCs) CD34 + Cells (Mixed Donor) CD34 + Cells (Single Donor) 5 million cells million cells 77.2 million cells million cells million cells 78 5 million cells million cells million cells million cells 78.5 Diabetes, Type II 1 million cells 762 Chronic Lymphocytic Leukemia (CLL) 1 million cells 763 CD36 + Cells 1 million cells 79 1 million cells 713 CD19 + B Cells 2.5 million cells Cryopreserved Human Bone Marrow Products 1 DESCRIPTION QUANTITY 5 million cells million cells 71.2 Mononuclear Cells (MNCs) 25 million cells 71 5 million cells million cells million cells million cells million cells 72.3 CD34 + Cells 1 million cells 72 2 million cells million cells 72.5 CD36 + Cells 7 1 million cells 73 CD15 + Cells.3 million cells 75 CD33 + Cells 5 million cells 76 Stromal Cells 7.75 million cells 722 Stromal Cells in ACF Medium 7.75 million cells million cells Pan-T Cells 15 million cells 714 CD4 + T Cells 15 million cells 715 CD4 + CD45RA + T Cells 15 million cells 717 CD8 + T Cells 5 million cells 716 CD14 + Monocytes 5 million cells 718 CD56 + NK Cells 1 million cells 719 Plasma 1 ml ml ml ml ml Certain cryopreserved products are only available in select territories. Please contact Product and Scientific Support (techsupport@stemcell.com) for further information. 2. High-resolution HLA typing and CMV status are available upon request. 3. Fresh products currently available in the United States and Canada (excluding Quebec). Please contact Product and Scientific Support (techsupport@stemcell.com) for further information. 4. A full size Leuko Pak typically contains 1.1 ±.3 x 1 1 cells and has a volume of approximately 12 ml 5. ACDA - Acid Citrate Dextrose Solution A; CP2D - Citrate-Phosphate-Double Dextrose; EDTA - Ethylenediaminetetraacetic Acid; Na Heparin - Sodium Heparin 6. Diseased states indicate s obtained from donors diagnosed with given condition. 7. Cultured Cell Product 17

18 IMMUNOLOGY RESEARCH PRODUCTS Human Cell Isolation Products Human T Cell Isolation by Negative Selection Naïve Pan T Cells EasySep Human Naïve Pan T Cell 96.1 ± 2.3% 1 x 1 9 cells CD4 (Catalog #616) CD8a (Catalog #622) CD45RO (Catalog #697) RF EasySep Human T Cell 96.7 ± 1.5% 1 x 1 9 cells EasySep HLA T Cell Enrichment Kit % 1 x 1 9 cells RF 1951HLA 1951HLARF Spleen, Lymph Node, EasySep Direct HLA Crossmatch T Cell 94.9 ± 1.5% 1 ml RF RF 3 T Cells EasySep HLA T Cell Enrichment Kit EasySep Direct Human T Cell % 2 ml CD2 (Catalog #67) CD4 (Catalog #616) CD8a (Catalog #622) 19951HLA 19951HLARF 95.3 ± 1.4% 1 ml RosetteSep Human T Cell Enrichment Cocktail % 4 ml ml 1561 RosetteSep HLA T Cell Enrichment Cocktail % 25 ml 1561HLA 1 ml 1581HLA RosetteSep HLA Lymphoid Cell Enrichment Kit > 85% 2 ml 15271HLA 4 Human T Cell Isolation by Positive Selection CD2 + Cells EasySep Human CD2 Positive Selection Kit EasySep HLA CD2 Positive Selection Kit % % 1 x 1 9 cells CD56 (Catalog #621) RF 18657HLA 18657HLARF CD3 + Cells EasySep Release Human CD3 Positive Selection Kit EasySep Human CD3 Positive Selection Kit II 98.7 ±.9% 99.2 ±.2% 1 x 1 9 cells CD4 (Catalog #616) CD8a (Catalog #622) RF, Buffy Coat EasySep HLA Chimerism Whole Blood CD3 Positive Selection Kit % 6 ml CD2 (Catalog #67) CD5 (Catalog #682) CD2 (Catalog #68) RF RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks Peripheral Blood Mononuclear Cells 2. Purities shown as either a range or mean ± SD. 3. This kit carries the CE mark and is available as a Class I in vitro diagnostic device (IVD) in the European Union, Canada, and Australia. To learn more about the regulatory status of this product in your specific region, contact info@stemcell.com. 4. This kit is designed to enrich only CD3 + lymphoid cells. 18 STEMCELL Technologies Inc.

19 Human CD4 + T Cell Isolation by Negative Selection CD4 + T Cells EasySep Human CD4 + T Cell EasySep Direct Human CD4 + T Cell RosetteSep Human CD4 + T Cell Enrichment Cocktail 94.8 ± 2.3% 1 x 1 9 cells RF 93.6 ± 2.5% 1 ml 4 ml CD4 (Catalog #616) CD8a (Catalog #622) ± 5.% 2 ml 1562 Human CD4 + T Cell Isolation by Positive Selection CD4 + T Cells EasySep Release Human CD4 Positive Selection Kit EasySep Human CD4 Positive Selection Kit II EasySep Human CD4 Positive Selection Kit 96.1 ± 4.1% 1 x 1 9 cells 9. ± 6.% 1 x 1 9 cells % 6 ml CD4 (Catalog #616) CD8a (Catalog #622) RF RF Human CD4 + T Cell Subset Isolation by Negative Selection Resting CD4 + T Cells EasySep Human Resting CD4 + T Cell 89. ± 5.3% 1 x 1 9 cells CD8 (Catalog #622) CD25 (Catalog #6153) RF Naïve CD4 + T Cells Memory CD4 + T Cells EasySep Human Naïve CD4 + T Cell II EasySep Human Memory CD4 + T Cell Enrichment Kit 96.6 ± 1.5% % 1 x 1 9 cells 1 x 1 9 cells CD4 (Catalog #616) CD45RO (Catalog #697) RF RF Regulatory T Cells Please see page 21. Human CD4 + T Cell Subset Isolation by Positive Selection Th1 Cells EasySep Human Th1 Cell % 1 x 1 9 cells CD4 (Catalog #616) RF Th17 Cells EasySep Human Th17 Cell Enrichment Kit II % 2 x 1 9 cells CD4 (Catalog #616) CD196 (Catalog #69) Central and Effector Memory CD4 + T Cells EasySep Human Central and Effector Memory CD4 + T Cell 92.3 ± 3.9% (CM) ± 4.1% (EM) 3 1 x 19 cells CD3 (Catalog #6127) CD45RO (Catalog #697) Regulatory T Cells Please see page 21. RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks Peripheral Blood Mononuclear Cells 2. Purities shown as either a range or mean ± SD. 3. CM - Central Memory; EM - Effector Memory 19

20 IMMUNOLOGY RESEARCH PRODUCTS Human CD8 + T Cell Isolation by Negative Selection EasySep Human CD8 + T Cell 85.6 ± 4.9% 1 x 1 9 cells RF CD8 + T Cells EasySep Direct Human CD8 + T Cell RosetteSep Human CD8 + T Cell Enrichment Cocktail 82.4 ± 4.9% 1 ml 4 ml CD4 (Catalog #616) CD8a (Catalog #622) ± 9.% 2 ml 1563 Human CD8 + T Cell Isolation by Positive Selection CD8 + Cells EasySep Human CD8 Positive Selection Kit II EasySep Human CD8 Positive Selection Kit 96.5 ± 2.4% 1 x 1 9 cells % 6 ml CD4 (Catalog #616) CD8a (Catalog #622) RF RF Human CD8 + T Cell Subset Isolation by Negative Selection Naïve CD8 + T Cells EasySep Human Naïve CD8 + T Cell II 93.7 ± 2.4% 1 x 1 9 cells CD8a (Catalog #622) CD45RO (Catalog #697) CD56 (Catalog #621) RF Memory CD8 + T Cells EasySep Human Memory CD8 + T Cell Enrichment Kit % 1 x 1 9 cells CD8a (Catalog #622) CD45RO (Catalog #697) RF Human CD8 + T Cell Subset Isolation by Positive Selection Central and Effector Memory CD8 + T Cells EasySep Human Central and Effector Memory CD8 + T Cell 86.8 ± 8.4% (CM) ± 6.5% (EM) 3 1 x 19 cells CD3 (Catalog #6127) CD45RO (Catalog #697) RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks Peripheral Blood Mononuclear Cells 2. Purities shown as either a range or mean ± SD. 3. CM - Central Memory; EM - Effector Memory 2 STEMCELL Technologies Inc.

21 Human Regulatory T Cell Isolation by Negative Selection CD4 + CD127 low T Cells CD4 + CD127 low CD49d - T Cells Whole Blood RosetteSep Human CD4 + CD127 low T Cell Enrichment Cocktail EasySep Human CD4 + CD127 low CD49d - Regulatory T Cell Enrichment Kit -- 2 ml % 3 2 x 1 9 cells CD4 (Catalog #616) CD25 (Catalog #6153) RF Human Regulatory T Cell Isolation by Positive Selection CD25 + Cells CD4 + CD127 low CD25 high T Cells EasySep Human CD25 Positive Selection and Depletion Kit Human CD4 + CD127 low CD25 + Regulatory T Cell 81-98% 1 x 1 9 cells CD4 (Catalog #616) CD25 (Catalog #6153) ± 2.1% 1 x 1 9 cells 1863 Human Gamma/Delta T Cell Isolation by Negative Selection Gamma/Delta T Cells EasySep Human Gamma/Delta T Cell % 1 x 1 9 cells CD2 (Catalog #67) CD4 (Catalog #616) CD8a (Catalog #622) RF RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks Peripheral Blood Mononuclear Cells 2. Purities shown as either a range or mean ± SD. 3. Purity data represents the CD3 + CD4 + CD127 low CD25 + FOXP3 + cell content of the enriched fraction. WALLCHART Regulatory T Cells Wallchart SCIENTIFIC POSTER Column-Free Isolation of Human Gamma/Delta T Cells

22 IMMUNOLOGY RESEARCH PRODUCTS Human B Cell and Subset Isolation by Negative Selection EasySep Human B Cell 95.1 ± 1.4% 1 x 1 9 cells CD19 (Catalog #65) RF EasySep HLA B Cell Enrichment Kit % 1 x 1 9 cells CD19 (Catalog #65) CD2 (Catalog #68) 1954HLA 1954HLARF B Cells 3 Spleen, Lymph Node, EasySep Direct HLA Crossmatch B Cell EasySep HLA B Cell Enrichment: Complete Processing Kit for Whole Blood 97 ± 3% 1 ml % 2 ml CD19 (Catalog #65) CD2 (Catalog #68) RF RF HLA 19954HLARF EasySep Direct Human B Cell RosetteSep Human B Cell Enrichment Cocktail RosetteSep HLA B Cell Enrichment Cocktail 95.3 ± 2.7% 1 ml ml % 2 ml CD19 (Catalog #65) ml CD2 (Catalog #68) 1564HLA % 1 ml 1584HLA Pan-B Cells 4 EasySep Human Pan-B Cell Enrichment Kit 9-99% 1 x 1 9 cells CD4 (Catalog #616) CD8a (Catalog #622) CD14 (Catalog #64) CD16 (Catalog #641) CD19 (Catalog #65) CD43 (Catalog #685) CD56 (Catalog #621) RF B Cells (without CD43 depletion) 5 EasySep Human B Cell Enrichment Kit II Without CD43 Depletion EasySep Direct Human B-CLL Cell % 1 x 1 9 cells RF CD19 (Catalog #65) CD2 (Catalog #68) 87. ± 7.6% 1 ml Naïve B Cells EasySep Human Naïve B Cell EasySep Direct Human Naïve B Cell 94.9 ± 2.2% 1 x 1 9 cells RF CD19 (Catalog #65) CD2 (Catalog #68) 91.8 ± 3.6% 1 ml Plasma Cells Bone Marrow RosetteSep Human Multiple Myeloma Cell Enrichment Cocktail > 95.% 4 ml CD138 (Syndecan-1) 2 ml (Catalog #63) RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks Peripheral Blood Mononuclear Cells 2. Purities shown as either a range or mean ± SD. 3. CD43 B cells from normal samples. 4. B cells including plasma cells from non-leukemia or lymphoma samples. 5. B cells from B cell leukemia or lymphoma samples, or other disease states in which B cells may express CD43, CD123, or CD36. Note that samples from normal healthy donors were used to obtain purity data. The purity of isolated cells is typically higher when processing samples that have an elevated frequency of B cells (e.g., CLL samples). 6. This kit carries the CE mark and is available as a Class I in vitro diagnostic device (IVD) in the European Union, Canada, and Australia. To learn more about the regulatory status of this product in your specific region, contact info@stemcell.com. 22 STEMCELL Technologies Inc.

23 Human B Cell and Subset Isolation by Positive Selection CD19 + Cells EasySep Release Human CD19 Positive Selection Kit EasySep Human CD19 Positive Selection Kit II 97.7 ± 2.3% 1 x 1 9 cells % 1 x 1 9 cells CD19 (Catalog #65) CD2 (Catalog #68) RF, Buffy Coat EasySep HLA Chimerism Whole Blood CD19 Positive Selection Kit % 6 ml RF CD2 + Cells EasySep Human CD2 Positive Selection Kit % 6 ml CD19 (Catalog #65) CD22 (Catalog #683) RF CD19 + CD27 + (Memory B) Cells EasySep Human Memory B Cell 97 ± 2% 1 x 1 9 cells CD19 (Catalog #65) CD27 (Catalog #616) CD138 + (Plasma) Cells, Bone Marrow, Bone Marrow EasySep Human CD138 Positive Selection Kit II EasySep Human and Bone Marrow CD138 Positive Selection Kit II % 2 x 1 9 cells % 6 ml CD138 (Syndecan-1) (Catalog #63) RF RF Human NK Cell Isolation by Negative Selection EasySep Human NK Cell 85. ± 8.% 1 x 1 9 cells RF NK Cells EasySep Direct Human NK Cell RosetteSep Human NK Cell Enrichment Cocktail % 1 ml CD56 (Catalog #621) ml ± 12.2% 2 ml 1565 Human NK Cell Isolation by Positive Selection CD56 + Cells Buffy Coat EasySep Human CD56 Positive Selection Kit II EasySep HLA Chimerism Buffy Coat CD56 Positive Selection Kit 94. ± 3.% 1 x 1 9 cells % 3 ml CD56 (Catalog #621) RF RF RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks Peripheral Blood Mononuclear Cells 2. Purities shown as either a range or mean ± SD. 23

24 IMMUNOLOGY RESEARCH PRODUCTS Human Total Lymphocyte Isolation by Negative Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR EasySep Direct Human Total Lymphocyte 96.7 ± 1.5% 1 ml RF Total Lymphocytes EasySep HLA Total Lymphocyte Enrichment: Complete Processing Kit for RosetteSep Human Total Lymphocyte Enrichment Cocktail % 2 ml 94. ± 2.% CD2 (Catalog #67) CD19 (Catalog #65) CD2 (Catalog #68) CD54 (NCAM) (Catalog #621) 19961HLA 19961HLARF 4 ml ml RosetteSep HLA Total Lymphocyte Enrichment Cocktail ml 15263HLA 2 1 ml 15283HLA 2 Human Lymphoid Cell Isolation by Positive Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR Lymphoid Cells EasySep Human Lymphoid Positive Selection Kit % 6 ml CD2 (Catalog #67) CD19 (Catalog #65) CD2 (Catalog #68) RF Human Innate Lymphoid Cell (ILC) Isolation by Negative Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR Group 2 Innate Lymphoid Cells Pan-Innate Lymphoid Cells Leukapheresis Sample Leukapheresis Sample RosetteSep Human ILC2 Enrichment Kit EasySep Human ILC2 Enrichment Kit EasySep Human Pan-ILC Enrichment Kit.44-23% (77-18 fold enrichment) 13-78% ( fold enrichment) 17-86% ( fold enrichment) 2 ml CD14 (Catalog #64) CD16 (Catalog #641) CD19 (Catalog #65) CD34 (Catalog #613) CD123 (Catalog #611) x 1 9 cells x 1 9 cells RF 3 Human Innate Lymphoid Cell (ILC) Isolation by Positive Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR Group 2 Innate Lymphoid Cells Leukapheresis Sample EasySep Human ILC % 2 x 1 9 cells CD14 (Catalog #64) CD16 (Catalog #641) CD19 (Catalog #65) CD34 (Catalog #613) CD123 (Catalog #611) RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks. 1. Purities shown as either a range or mean ± SD. 2. This kit carries the CE mark. 3. These kits enrich for group1, group 2, and group 3 innate lymphoid cells (ILC1, ILC2, and ILC3). 24 STEMCELL Technologies Inc.

25 Human Dendritic Cell Isolation by Negative Selection Pan-Dendritic Cells Myeloid Dendritic Cells Plasmacytoid Dendritic Cells EasySep Human Pan-DC Pre-Enrichment Kit EasySep Human Myeloid DC Enrichment Kit EasySep Human Plasmacytoid DC Enrichment Kit % 1 x 1 9 cells % 2 x 1 9 cells % 2 x 1 9 cells CD14 (Catalog #64) CD19 (Catalog #65) CD2 (Catalog #68) CD34 (Catalog #613) CD56 (Catalog #621) RF RF RF Human Monocyte Isolation by Negative Selection EasySep Human Monocyte Enrichment Kit without CD16 Depletion % 1 x 1 9 cells RF 3 Monocytes EasySep Human Monocyte EasySep Direct Human Monocyte 87. ± 5.% 1 x 1 9 cells CD14 (Catalog #64) CD16 (Catalog #641) CD36 (Catalog #684) RF 82.2 ± 8.4% 1 ml RosetteSep Human Monocyte Enrichment Cocktail % 4 ml ml 1568 Human Granulocyte and Subset Isolation by Negative Selection Pan-Granulocytes PMNC EasySep Human Pan-Granulocyte EasySep Direct Human Pan-Granulocyte % 1 x 1 9 cells CD16 (Catalog #641) 19259RF CD66b (Catalog #686) CD123 (Catalog #611) 98.4 ± 1.5% 1 ml Basophils PMNC EasySep Human Basophil 94. ± 2.5% 1 x 1 9 cells CD123 (Catalog #611) RF Neutrophils Eosinophils PMNC PMNC EasySep Human Neutrophil Enrichment Kit EasySep Direct Human Neutrophil EasySep Human Eosinophil % 1 x 1 9 cells RF CD11b (Catalog #64) 95.3 ± 1.4% 1 ml CD16 (Catalog #641) CD66b (Catalog #686) 96.5 ± 2.5% 1 x 1 9 cells RF RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks Peripheral Blood Mononuclear Cells; PMNC - Polymorphonuclear Cells 2. Purities shown as either a range or mean ± SD. 3. These kits isolate CD14 + CD16 + monocytes. 25

26 IMMUNOLOGY RESEARCH PRODUCTS Human Myeloid Cell Isolation by Negative Selection Myeloid Cells RosetteSep HLA Myeloid Cell Enrichment Kit % 2 ml CD11b (Catalog #64) CD33 (Catalog # 696) CD66b (Catalog #686) 15272HLA Human Myeloid Cell Isolation by Positive Selection CD14 + Cells Buffy Coat EasySep Human CD14 Positive Selection Kit II EasySep HLA Chimerism Buffy Coat CD14 Positive Selection Kit % 1 x 1 9 cells 93. ± 6.4% 3 ml CD14 (Catalog #64) CD36 (Catalog #684) RF RF CD15 + Cells PMNC, Buffy Coat EasySep Human CD15 Positive Selection Kit EasySep HLA Chimerism Whole Blood CD15 Positive Selection Kit 98.8 ±.8% 1 x 1 9 cells 99.2 ± 1.1% 6 ml RF RF CD33 + Cells Lysed EasySep Human CD33 Positive Selection Kit EasySep HLA Chimerism Whole Blood CD33 Positive Selection Kit % 1 x 1 9 cells % 6 ml CD66b (Catalog #686) CD14 (Catalog #64) RF RF CD33 + CD66b + (Myeloid) Cells Lysed, Buffy Coat EasySep Human Myeloid Positive Selection Kit EasySep HLA Chimerism Whole Blood Myeloid Positive Selection Kit % 1 x 1 9 cells CD66b (Catalog #686) CD14 (Catalog #64) 94.5 ± 4.1% 6 ml CD33 (Catalog #696) RF RF CD66b + Cells (Granulocytes) EasySep Human CD66b Positive Selection Kit % 6 ml CD66b (Catalog #686) RF CD271 + Cells Bone Marrow EasySep Human CD271 Selection Kit -- 2 x 1 9 cells Dextran (Catalog #626) RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks Peripheral Blood Mononuclear Cells; PMNC - Polymorphonuclear Cells 2. Purities shown as either a range or mean ± SD. 26 STEMCELL Technologies Inc.

27 Human Hematopoietic Progenitor Cell Isolation by Negative Selection Mobilized, BMMC EasySep Human Progenitor Cell Enrichment Kit 42. ± 5. fold CD34 + cell enrichment (bone marrow) 1 x 1 9 cells RF, CBMC EasySep Human Progenitor Enrichment Kit with Platelet Depletion % 1 x 1 9 cells RF 3 Hematopoietic Progenitors Bone Marrow Cord Blood RosetteSep Human Bone Marrow Progenitor Cell Pre-Enrichment Cocktail RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail Complete RosetteSep Human Cord Blood Progenitor Enrichment Kit 25. ± 1. fold CD34 + cell enrichment 29. ± 9.% 4 ml ml ml CD34 (Catalog #613) ml ± 9.% 5 ml Lineage Negative Cells Cord Blood RosetteSep Human Cord Blood Debulking Cocktail 5. ± 1.% (CD34 + cells) 4 ml ml Human CD34 + Hematopoietic Progenitor Cell Isolation by Positive Selection Mobilized, CBMC, BMMC EasySep Human CD34 Positive Selection Kit % 5 x 1 9 cells RF 5, Buffy Coat EasySep Human CD34 Positive Selection Kit % 75 ml whole blood 37 ml buffy coat RF CD34 + Cells Complete Kit for Human CD34 + Cells % 12 ml CD34 (Catalog #613) RF Cord Blood EasySep Human Cord Blood CD34 Positive Selection Kit II EasySep Human Cord Blood CD34 Positive Selection Kit III 91. ± 9.% 1 ml 87. ± 12% 1 ml , RF 6, , RF 6,8 hesc and hipsc Cultures EasySep hesc-derived CD34 Positive Selection Kit % 5 x 1 9 cells RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks Peripheral Blood Mononuclear Cells; CBMC - Cord Blood Mononuclear Cells; BMMC - Bone Marrow Mononuclear Cells; hesc - Human Embryonic Stem Cell; hipsc - Human Induced Pluripotent Stem Cell 2. Purities shown as either a range or mean ± SD. Purity data for 1856, 1886, and 1586 are reported relative to viable CD45 + cells. 3. This product is designed for use with samples that contain large numbers of platelets. 4. This product is recommended for debulking cord blood of lineage positive cells prior to freezing. 5. These kits are for use with previously frozen cord blood mononuclear cells. For isolation of CD34 + cells from fresh cord blood, please use and 17896RF. 6. These kits are for use with fresh cord blood. For isolation of CD34 + cells from previously frozen cord blood mononuclear cells, please use 1856 and 1856RF. 7. This kit contains antibodies for platelet depletion and is recommended for preenrichment of CD34 + cells from cord blood samples that contain large amounts of platelets. Platelets may affect the quality and purity of CD34 + cells if not depleted. 8. This kit does not contain antibodies for platelet depletion and is recommended for preenrichment of CD34 + cells from cord blood samples that contain few platelets or when platelet depletion is not desired. 27

28 IMMUNOLOGY RESEARCH PRODUCTS Isolation of Other Human Cell Types by Negative Selection CELL TYPE SOURCE PRODUCT NAME PURITY FOR EasySep Direct Human CTC Enrichment Kit log depletion 1 ml CD326 (EpCAM) (Catalog #119) Circulating Epithelial Tumor Cells RosetteSep CTC Enrichment Cocktail Containing Anti-CD36 RosetteSep CTC Enrichment Cocktail Containing Anti-CD log depletion log depletion 4 ml ml 4 ml CD326 (EpCAM) (Catalog #119) ml Other Cell Types (Custom) Any Source EasySep Human Custom Enrichment Kit RosetteSep Human Custom Cocktail -- As requested RF 1 -- As requested Isolation of Other Human Cell Types by Positive Selection CELL TYPE SOURCE PRODUCT NAME PURITY FOR EasySep Human Custom Positive Selection Kit -- As requested RF 2 EasySep Release Human Biotin Positive Selection Kit -- 1 x 1 9 cells Goat Anti-Mouse IgG (H+L) (Catalog #6138) EasySep Release Human PE Positive Selection Kit -- 1 x 1 9 cells Other Cell Types (Custom) Any Source EasySep Human PE Positive Selection Kit II EasySep Human FITC Positive Selection Kit II -- 1 x 1 9 cells -- 1 x 1 9 cells RF 4 5 x 1 9 cells , RF 6 EasySep Human Biotin Positive Selection Kit II -- 1 x 1 9 cells Dextran (Catalog #626) RF 3 EasySep Human APC Positive Selection Kit II EasySep Human "Do-It-Yourself" Selection Kit II -- 1 x 1 9 cells -- 1 x 1 9 cells RF RF 8 RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks. 1. Isolate any human cell type by negative selection. 2. Isolate any human cell type by positive selection. 3. Use with biotinylated antibodies. 4. Use with PE-conjugated antibodies. 5. This product includes 5 x Use with FITC-conjugated antibodies. 7. Use with APC-conjugated antibodies. 8. Use your own mouse IgG 1 antibodies. 28 STEMCELL Technologies Inc.

29 Human Cell Depletion Products Human T Cell and Subset Depletion CELL TYPE SOURCE 1 PRODUCT NAME PURITY FOR CD3 + Cells RosetteSep Human CD3 Depletion Cocktail Typically 3. log depletion 4 ml ml CD4 + Cells RosetteSep Human CD4 Depletion Cocktail Typically 2. log depletion 4 ml CD4 (Catalog #616) 2 ml CD8 + Cells RosetteSep Human CD8 Depletion Cocktail Typically 2. log depletion 4 ml CD8a (Catalog #622) ml CD25 + Cells EasySep Human CD25 Positive Selection and Depletion Kit Typically 1.3 log depletion 1 x 1 9 cells CD25 (Catalog #6153) Human Monocyte Depletion CELL TYPE SOURCE PRODUCT NAME PURITY FOR Monocytes RosetteSep Human Monocyte Depletion Cocktail Typically 2.8 log depletion 4 ml CD14 (Catalog #64) CD16 (Catalog #641) ml CD36 (Catalog #684) Human Granulocyte and Subset Depletion CELL TYPE SOURCE PRODUCT NAME PURITY FOR Granulocytes RosetteSep Human Granulocyte Depletion Cocktail RosetteSep HLA Granulocyte Depletion Cocktail < 1% Granulocytes < 1% Granulocytes 4 ml ml 25 ml CD11b (Catalog #64) CD16 (Catalog #641) CD66b (Catalog #686) HLA 1 ml 15684HLA IgE + Cells RosetteSep Anti-Human IgE Tetramer -- 4 ml 1523 Depletion of Other Human Cell Types CELL TYPE SOURCE 1 PRODUCT NAME PURITY FOR EasySep Human CD45 Depletion Kit Typically 4. log depletion 2 x 1 9 cells RF 2 CD45 + Cells EasySep Human CD45 Depletion Kit RosetteSep Human CD45 Depletion Cocktail log depletion Typically 3.6 log depletion 1 ml RF 2 4 ml ml GlyA + Cells EasySep Human Positive Glycophorin A Depletion Cocktail x 1 9 cells GlyA (Catalog #1423) RF Red Blood Cells EasySep Red Blood Cell Depletion Kit Typically <1% red blood cells 1 ml GlyA (Catalog #1423) RF Dead Cells (Annexin V + ) Tissue Preparations EasySep Dead Cell (Annexin V + ) Removal Kit -- 1 x 1 9 cells RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks Peripheral Blood Mononuclear Cells 2. CD45 depletion is useful to enrich circulating non-hematopoietic tumor cells from peripheral blood. 29

30 IMMUNOLOGY RESEARCH PRODUCTS Mouse Cell Isolation Products Mouse T Cell and Subset Isolation by Negative Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR T Cells Spleen EasySep Mouse T Cell % 1 x 1 9 cells Pan-Naïve T Cells Spleen EasySep Mouse Pan-Naïve T Cell % 1 x 1 9 cells CD3e (Catalog #615) CD9 (Catalog #624) RF RF CD4 + T Cells Spleen EasySep Mouse CD4 + T Cell % 1 x 1 9 cells CD3e (Catalog #615) CD4 (Catalog #617) RF Naïve CD4 + T Cells Memory CD4 + T Cells Spleen Spleen EasySep Mouse Naïve CD4 + T Cell EasySep Mouse Memory CD4 + T Cell % 1 x 1 9 cells % 1 x 1 9 cells CD4 (Catalog #617) CD44 (Catalog #668) CD62L (Catalog #619) RF RF CD8 + T Cells Spleen EasySep Mouse CD8 + T Cell % 1 x 1 9 cells CD3e (Catalog #615) CD8a (Catalog #623) RF Naïve CD8 + T Cells Spleen EasySep Mouse Naïve CD8 + T Cell % 1 x 1 9 cells CD8a (Catalog #623) CD44 (Catalog #668) CD62L (Catalog #619) RF Mouse T Cell and Subset Isolation by Positive Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR CD9.2 + (Thy 1.2) Cells Spleen EasySep Mouse CD9.2 Positive Selection Kit II % 2 x 1 9 cells CD9.2 (Catalog #6115) RF CD9.1 + (Thy 1.1) Cells Spleen, Lymph Node, EasySep Mouse CD9.1 Positive Selection Kit 93.5 ± 3.9% 2 x 1 9 cells CD9 (Catalog #624) RF CD4 + Cells Spleen EasySep Mouse CD4 Positive Selection Kit II 98.6 ±.4% 2 x 1 9 cells CD3e (Catalog #615) CD4 (Catalog #629) RF CD4 + CD62L + Cells Spleen EasySep Mouse CD4 + CD62L + T Cell % 1 x 1 9 cells CD4 (Catalog #617) CD44 (Catalog #668) RF CD4 + CD25 + Cells Spleen EasySep Mouse CD4 + CD25 + Regulatory T Cell II % 1 x 1 9 cells CD4 (Catalog #629) CD4 (Catalog #617) CD25 + Cells Spleen EasySep Mouse CD25 Regulatory T Cell Positive Selection Kit % 1 x 1 9 cells CD4 (Catalog #617) RF CD8a + Cells Spleen EasySep Mouse CD8a Positive Selection Kit II 96.3 ± 1.4% 2 x 1 9 cells CD3e (Catalog #615) CD8a (Catalog #623) RF RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks. 1. Purities shown as either a range or mean ± SD. 3 STEMCELL Technologies Inc.

31 Mouse B Cell Isolation by Negative Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR B Cells Spleen EasySep Mouse B Cell % 1 x 1 9 cells CD19 (Catalog #66) RF 2 Pan-B Cells Spleen EasySep Mouse Pan-B Cell % 1 x 1 9 cells CD19 (Catalog #66) CD138 (Catalog #635) RF 3 Mouse B Cell and Subset Isolation by Positive Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR CD19 + Cells Spleen EasySep Mouse CD19 Positive Selection Kit II % 2 x 1 9 cells CD19 (Catalog #66) RF CD138 + Cells Spleen, Lymph Node, Bone Marrow EasySep Mouse CD138 Positive Selection Kit 81.5 ± 4.9% 2 x 1 9 cells CD138 (Catalog #635) CD45R (Catalog #619) CD267 (Catalog #6116) RF Mouse NK Cell Isolation by Negative Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR NK Cells Spleen EasySep Mouse NK Cell % 1 x 1 9 cells CD3e (Catalog #615) CD49b (Catalog #62) RF Mouse NK Cell Isolation by Positive Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR COMPATIBLE STAINIG CD49b + Cells Spleen EasySep Mouse CD49b Positive Selection Kit 74-9% 2 x 1 9 cells Dextran (Catalog # 626) RF Mouse Innate Lymphoid Cell (ILC) Isolation by Negative Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR Group 2 Innate Lymphoid Cells Lung EasySep Mouse ILC2 Enrichment Kit % (3-11 fold enrichment) 1 x 1 9 cells CD45 (Catalog #63) CD9.2 (Catalog #6115) CD3e (Catalog #615) CD11b (Catalog #61) CD11c (Catalog #62) CD19 (Catalog #6112) Gr-1 (Catalog #628) CD161 (Catalog #613) TER119 (Catalog #633) TCR Gamma/Delta (Catalog #614) RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks. 1. Purities shown as either a range or mean ± SD. 2. This kit is designed for the isolation of conventional (B-2) B cells only. 3. This kit is designed to isolate all B cells, including conventional (B-2) B cells, B-1 B cells, and plasma cells. This kit is compatible for use with cells from disease models where malignant cells express CD43 or CD11b. 31

32 IMMUNOLOGY RESEARCH PRODUCTS Mouse Dendritic Cell Isolation by Negative Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR Pan-Dendritic Cells Spleen EasySep Mouse Pan-DC Enrichment Kit % 2 x 1 9 cells CD3e (Catalog #615) CD11c (Catalog #62) CD19 (Catalog #66) F4/8 (Catalog #627) Ly6G (Catalog #631) TER119 (Catalog #633) NK1.1 (Catalog #613) RF Plasmacytoid Dendritic Cells Spleen EasySep Mouse Plasmacytoid DC % 2 x 1 9 cells CD11c (Catalog #62) RF Mouse Monocyte Isolation by Negative Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR Monocytes, Bone Marrow EasySep Mouse Monocyte 94.2 ± 1.5% 1 x 1 9 cells CD11b (Catalog #61) F4/8 (Catalog #627) RF Mouse Neutrophil Isolation by Negative Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR Neutrophils, Bone Marrow EasySep Mouse Neutrophil Enrichment Kit % () % (Bone Marrow) 1 x 1 9 cells CD11b (Catalog #61) Gr-1 (Catalog #628) Ly6G (Catalog #631) RF RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks. 1. Purities shown as either a range or mean ± SD. 32 STEMCELL Technologies Inc.

33 Mouse Myeloid Cell Isolation by Positive Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR CD11b + Cells Spleen, Bone Marrow, Lung, Brain EasySep Mouse CD11b Positive Selection Kit II 92.6 ± 3.% (Spleen) 98.4 ± 2.3% (Bone Marrow) 86.9 ± 7.6% (Lung) 94.2 ± 4.% (Brain) 2 x 1 9 cells CD11b (Catalog #61) RF CD11c + Cells Spleen, Cultured Bone Marrow EasySep Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium EasySep Mouse CD11c Positive Selection Kit II 86.8 ± 9.7% 2 x 1 9 cells % 2 x 1 9 cells CD11c (Catalog #62) RF RF Mouse Hematopoietic Progenitor Isolation by Negative Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR Hematopoietic Progenitor Cells Bone Marrow EasySep Mouse Hematopoietic Progenitor Cell % 1 x 1 9 cells CD3 (Catalog #615) CD11b (Catalog #61) CD19 (Catalog #66) CD45R (Catalog #619) Gr-1 (Catalog #628) TER119 (Catalog #633) RF Mouse Hematopoietic Progenitor Isolation by Positive Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR SCA1 + Cells Bone Marrow EasySep Mouse Sca1 Positive Selection Kit % 2 x 1 9 cells Sca1 (Catalog #632) RF CD117 + (c-kit) Cells Bone Marrow EasySep Mouse CD117 (ckit) Positive Selection Kit % 2 x 1 9 cells Dextran (Catalog #626) RF RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks. 1. Purities shown as either a range or mean ± SD. 33

34 IMMUNOLOGY RESEARCH PRODUCTS Isolation of Other Mouse Cell Types by Negative Selection CELL TYPE SOURCE PRODUCT NAME PURITY 1 FOR Human Cells from Xenograft-Recipient Mice Bone Marrow, Spleen and Blood EasySep Mouse/Human Chimera % 1 x 1 9 cells CD45R (Catalog #619) TER119 (Catalog #633) Other Cell Types (Custom) Any Source EasySep Mouse Custom Enrichment Kit -- As requested RF 2 Isolation of Other Mouse Cell Types by Positive Selection CELL TYPE SOURCE PRODUCT NAME PURITY FOR EasySep Mouse Custom Positive Selection Kit -- As requested RF 3 EasySep Release Mouse Biotin Positive Selection Kit -- 1 x 1 9 cells Goat Anti-Mouse IgG (H+L) (Catalog #6138) EasySep Release Mouse PE Positive Selection Kit -- 1 x 1 9 cells Other Cell Types (Custom) Any Source EasySep Mouse PE Positive Selection Kit -- 1 x 1 9 cells RF x 1 9 cells ,6 EasySep Mouse FITC Positive Selection Kit -- 1 x 1 9 cells Dextran (Catalog #626) RF 7 EasySep Mouse Biotin Positive Selection Kit -- 1 x 1 9 cells RF 4 EasySep Mouse APC Positive Selection Kit -- 1 x 1 9 cells RF 8 RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks. 1. Purities shown as either a range or mean ± SD. 2. Isolate any mouse cell type by negative selection. 3. Isolate any mouse cell type by positive selection. 4. Use with biotinylated antibodies. 5. Use with PE-conjugated antibodies. 6. This product includes 5 x Use with FITC-conjugated antibodies. 8. Use with APC-conjugated antibodies. WALLCHART Frequencies & Percentages of Mouse Immune Cell Types 34 STEMCELL Technologies Inc.

35 Other Species Cell Isolation Products Rat Cell Isolation by Negative or Positive Selection, or Depletion CELL TYPE SELECTION METHOD SOURCE PRODUCT NAME PURITY 1 FOR T Cells Negative Spleen,, Lymph Node EasySep Rat T Cell 98.1 ± 1.2% 1 x 1 9 cells RF CD4 + T Cells Negative Spleen,, Lymph Node EasySep Rat CD4 + T Cell 97.2 ± 1.2% 1 x 1 9 cells RF CD8 + T Cells Negative Spleen,, Lymph Node EasySep Rat CD8 + T Cell 92.3 ± 3.1% 1 x 1 9 cells RF B Cells Negative Spleen,, Lymph Node EasySep Rat B Cell 94.1 ± 3.8% 1 x 1 9 cells RF Other Cell Types (Custom) Negative Any Source EasySep Rat Custom Enrichment Kit -- As requested RF 2 Any Cell Type (Custom) Positive Any Source EasySep Rat Custom Positive Selection Kit -- As requested RF 3 IgM + Cells Depletion Spleen,, Lymph Node EasySep Rat IgM Depletion Kit Typically 1.6 log depletion 1 x 1 9 cells RF Non-Human Primate Cell Isolation by Negative or Positive Selection CELL TYPE SELECTION METHOD SOURCE PRODUCT NAME Any Cell Type (Custom) Negative Any Source EasySep Non-Human Primate Custom Enrichment Kit Positive Any Source EasySep Non-Human Primate Custom Positive Selection Kit RF RF 5 Isolation of Cells from Other Species by Positive Selection CELL TYPE SOURCE PRODUCT NAME FOR EasySep PE Positive Selection Kit 1 x 1 9 cells , 17684RF 6 EasySep FITC Positive Selection Kit 1 x 1 9 cells , 17682RF 7 EasySep Biotin Positive Selection Kit 1 x 1 9 cells , 17683RF 8 Any Cell Type Any Source EasySep APC Positive Selection Kit 1 x 1 9 cells , 17681RF 9 EasySep Do-It-Yourself Selection Kit 1 x 1 9 cells , 17698RF 1 EasySep Anti-Rat IgG2a Positive Selection Kit 2 x 1 9 cells , 1899RF 11 EasySep Anti-Rat IgG2b Positive Selection Kit 2 x 1 9 cells , 18992RF 12 EasySep Anti-Rat IgM Positive Selection Kit 2 x 1 9 cells , 18994RF 13 RoboSep Reagent Kits (RF) contain an EasySep Selection Kit with RoboSep Buffer and 1-2 boxes of RoboSep Tip Racks. 1. Purities shown as either a range or mean ± SD 2. Isolate any rat cell type by negative selection. 3. Isolate any rat cell type by positive selection. 4. Isolate any non-human primate cell type by negative selection. 5. Isolate any non-human primate cell type by positive selection. 6. Use with PE-conjugated antibodies. 7. Use with FITC-conjugated antibodies. 8. Use with biotinylated antibodies. 9. Use with APC-conjugated antibodies. 1. Use with your own mouse IgG 1 antibody. 11. Use with your own rat IgG2a antibody. 12. Use with your own rat IgG2b antibody. 13. Use with your own rat IgM antibody. 35

36 IMMUNOLOGY RESEARCH PRODUCTS Cell Isolation Magnets and Accessories EasySep Magnets PRODUCT NAME EasySep Magnet 18 The Big Easy EasySep Magnet 181 Easy 5 EasySep Magnet 182 EasyEights EasySep Magnet 1813 EasyPlate EasySep Magnet 1812 EasySep Magnet Stands PRODUCT NAME UNIT SIZE EasySep Magnet Stands EasySep Multistand Separate up to 4 samples at once using the EasySep Multistand. The multistand is designed to hold up to 4 EasySep Magnets or 4 The Big Easy EasySep Magnets. (Catalog #181) EasySep EasyStand Separate up to 6 samples at once using the EasySep EasyStand. Each EasyStand can hold a single EasySep Magnet, and up to 6 EasyStands can be linked together. (Catalog #1813) EasySep EasyStand stand EasySep EasyStand with EasySep Magnet stand & 1 x 18 Density Media 4 x EasySep EasyStand stands PRODUCT NAME UNIT SIZE 4 x EasySep EasyStand with EasySep Magnets stands & 4 x 18 6 x EasySep EasyStand stands Lymphoprep ml 5 ml 4 x 25 ml 6 x 5 ml 6 x EasySep EasyStand with EasySep Magnets stands & 6 x 18 EasySep Multistand stand OptiPrep ml HetaSep ml 1 ml EasySep Multistand with EasySep Magnets EasySep Multistand with "The Big Easy" Magnets stand & 4 x 18 1 stand & 4 x 181 RosetteSep DM-L ml RosetteSep DM-M ml SpinSep Density Medium ml SepMate Products Enzymes for Tissue Dissociation PRODUCT NAME SepMate -15 (IVD 3 ) BLOOD VOLUME PROCESSED (ML).5-5 UNIT SIZE 1 tubes 5 tubes SepMate -15 (RUO 4 ) tubes SepMate -5 (IVD 3 ) tubes 5 tubes SepMate -5 (RUO 4 ) tubes 1. The EasySep EasyStand facilitates the separation of one or more samples at one time. Up to 6 samples can be isolated simultaneously using EasySep magnets (sold separately) and linkable EasySep EasyStand units. 2. The EasySep Multistand facilitates the separation of up to 4 samples at one time. This system is compatible with EasySep magnets and "The Big Easy" EasySep magnets (magnets sold separately). 3. SepMate (IVD) is only available in select regions where it is registered as an In Vitro Diagnostic (IVD) device for the isolation of mononuclear cells from human whole blood or cord blood by density gradient centrifugation. 4. SepMate for Research Use Only (RUO) is available in regions where SepMate is not registered as an IVD device. PRODUCT NAME UNIT SIZE ACCUTASE ml Collagenase Type I ml Collagenase Type IV ml Collagenase/Hyaluronidase (1X) ml Dispase (1 mg/ml) ml DNase I (1 mg/ml) 79 1 ml Spleen Dissociation Medium x 4 ml Trypsin-EDTA (.5%) ml Trypsin in Citrate Saline 74 1 ml 36 STEMCELL Technologies Inc.