Institute of Science & Technology in Medicine Control of Substance Hazardous to Health COSHH assessment

Transcription

1 Institute of Science & Technology in Medicine Control of Substance Hazardous to Health COSHH assessment Title of Experiment/Procedure: Reverse Transcription using the High Capacity cdna Reverse Transcription Kit & RNase Inhibitor (John Glossop) Date of Assessment: 12/06/09 Names & Status of Worker: Dr John Glossop Name of hazardous substances to be used 1. Multiscribe Rev Transcriptase, glycerol Type of Hazard or Biological Hazards identified NOTE: enter skin contact or absorption, eye contact, inhalation, injection, ingestion, where relevant. i, Irritant R36/37/38 irritant to eyes, respiratory S24 avoid contact with skin S26 in case of contact with eyes, rinse immediately with plenty of water and seek medical advice S37 wear suitable gloves S60 this material and its container must be disposed of as hazardous waste Approximate quantity and the likelihood of exposure, e.g. high, medium, low RT Buffer Component 1, i, Irritant R36/38 irritant to eyes and skin

2 Component 2, n, Harmful; i, Irritant R36/37/38 irritant to eye, respiratory R52 harmful to aquatic organisms R22 harmful is swallowed 3. DNTP Mix Skin contact wash off with plenty of water. Get medical attention if symptoms occur Eye contact rinse immediately with plenty of water, also under the eyelids, for at if Inhalation move to fresh air if Ingestion if swallowed, DO NOT induce vomiting unless directed to do so by medical personnel. Rinse mouth with water.. Get medical attention if 4. Random Primers Skin contact wash off with plenty of water. Get medical attention if symptoms

3 occur Eye contact rinse immediately with plenty of water, also under the eyelids, for at if Inhalation move to fresh air if Ingestion if swallowed, DO NOT induce vomiting unless directed to do so by medical personnel. Rinse mouth with water.. Get medical attention if 5. RNase Inhibitor, glycerol i, Irritant R36/37/38 irritant to eyes, respiratory S24 avoid contact with skin S26 in case of contact with eyes, rinse immediately with plenty of water and seek medical advice S37 wear suitable gloves S60 this material and its container must be disposed of as hazardous waste Overall risk assessment of the substances Very high High Medium Carcinogen category

4 1. Multiscribe Rev Transcriptase, Glycerol RT Buffer Component 1, Component 2, 3. DNTP Mix 4. Random Primers 5. RNase Inhibitor Information source: MSDS assessments from supplier (Applied Biosystems; product codes , , , , 5. N ) and also from other online sources Institute of Science & Technology in Medicine Control of Substance Hazardous to Health COSHH assessment Biological Safety Cabinet Control Measures adopted (personal protective equipment) Fume cupboard Good Ventilation Safety Goggles Gloves Face Mask/ If special, state details Dust mask Lab coat

5 Gloves Face protection Spillage Disinfectant Spillage kit Mop up Mix with sand Method of Disposal Waste bottle Drain Yellow bag Autoclave No hazard Emergency procedures In case of fire In case of water failure In case of electricity failure Emergency contact name Release of carbon oxides (1 5 ), nitrogen oxides (2), and gaseous hydrogen chloride (2) on decomposition; No specific instructions No specific instructions Dr. John Glossop Emergency phone number Signatures Name and signature Date Assessor Dr John Glossop 12 June 2009 Supervisor or COSHH Supervisor Dr Ying Yang 12 June 2009