MARIA PickYourPlex Multiplex Array for Indoor Allergens Kit 96 Well Plate Assay
|
|
- Arabella Cole
- 5 years ago
- Views:
Transcription
1 MARIA PickYourPlex Multiplex Array for Indoor Allergens Kit 96 Well Plate Assay Indoor Biotechnologies, Inc. 700 Harris Street, Charlottesville, VA United States Tel: (434) Fax:(434) Indoor Biotechnologies Ltd. Vision Court Caxton Place Cardiff, Wales CF23 8HA United Kingdom Tel: +44 (0) Indoor Biotechnologies, India Private Limited Bangalore Bioinnovation Centre, BioTech Park, Electronic City Phase 1 Bangalore , India Tel: info@inbioindia.com INDOOR and are Registered Trademarks of INDOOR Biotechnologies, Ltd., Manchester U.K. An InBio product 16 Storage: The MARIA kit should be stored at 4 C (QC samples and UAS should be frozen following receipt) For Research Use Only: Not for Diagnostic or Therapeutic Use 2012, Indoor Biotechnologies, Inc.
2 12. References 1. King EM, Filep S, Smith B. Platts-Mills TAE, Hamilton RG, Schmechel D, Sordillo JE, Milton D, van Ree RR, Krop EJM, Heederik DJJ, Metwali N, Thorne PS, Sever ML, Zeldin DC, Calatroni A, Arbes SJ, Mitchell HE, Chapman MD. A multi-center ring trial of allergen exposure assessment using fluorescent multiplex array technology. J Immunol Meths 2012;Oct 22. [Epub ahead of print]. 2. Filep S, Tsay A, Vailes LD, Gadermaier G, Ferreira F, Matsui E, King E, Chapman MD. A multi-allergen standard for the calibration of immunoassays: CREATE principles applied to eight purified allergens. Allergy 2012;67: Permaul P, Hoffman E, Fu C, Sheehan W, Baxi S, Gaffin J, Lane J, Bailey A, King E, Chapman M, Gold D, Phipatanakul W. Allergens in urban schools and homes of children with asthma. Pediatr Allergy Immunol. 2012;23: Samadi S, Heederik DJ, Krop EJ, Jamshidifard AR, Willemse T, Wouters IM. Allergens and endotoxin exposure in a companion animal hospital. Occup Environ Med. 2010;67: Wright GR, Howieson S, McSharry C, McMahon AD, Chaudhuri R, Thompson J, Donnelly I, Brooks RG, Lawson A, Jolly L, McAlpine L, King EM, Chapman MD, Wood S, Thomson N. Effect of improved home ventilation on asthma control and house dust mite allergen levels. Allergy 2009;64: Earle CD, King EM, Tsay A, Pittman K, Saric B, Vailes L, Godbout R, Oliver KG, Chapman MD. High-throughput fluorescent multiplex array for indoor allergen exposure assessment. J Allergy Clin Immunol. 2007;119:
3 11. Technical Notes MARIA Multiplex Array for Indoor Allergens 1. Intended use 2. Reagent Lots Supplied 3. Storage Conditions 4. Materials Required but Not Provided 5. Technical Notes 6. Certificate of Analysis 7. MARIA Protocol 8. MARIA 96 Well Plate Layout 9. Sample Curves 10. Assay Performance 11. Assay Workflow 12. References By opening the packaging containing this Kit (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Kit in any manner, you are consenting and agreeing to be bound by the following terms and conditions. You are also agreeing that the following terms and conditions constitute a legally valid and binding contract that is enforceable against you. If you do not agree to all of the terms and conditions set forth below, you must promptly return this Kit for a full refund prior to using it in any manner. You, the customer, acquire the right under Luminex Corporation's patent rights, if any, to use this Kit or any portion of this Kit, including without limitation the microsphere beads contained herein, only with Luminex Corporation s laser based fluorescent analytical test instrumentation marketed under the name Luminex Instrument. The Luminex Instrument refers to Luminex 100, Luminex 200 and other Luminex Instruments available from Luminex Corporation and from authorized distributors including Bio-Rad Laboratories (Hercules, CA), Qiagen Corporation (Valencia, CA) and MiraiBio (South San Francisco, CA). Estimated Assay Time Required: Sample Preparation: 1 hour; Incubation: 2.5 hours; Plate Reading: 1 hour 14 3
4 9. Sample Curves (cont.) 1. Intended Use This is a multiplex assay kit manufactured by INDOOR Biotechnologies Inc. to be used for the simultaneous quantitative determination of up to eleven common indoor allergens: house dust mite allergens Der p 1 (Dermatophagoides pteronyssinus), Der f 1 (Dermatophagoides farinae) and Mite Group 2, animal allergens Fel d 1 (cat, Felis domesticus), Can f 1 (dog, Canis familiaris), Mus m 1 (mouse, Mus musculus), Rat n 1 (rat, Rattus norwegicus), German cockroach, Bla g 2 (Blattella germanica), mold allergen Alt a 1 (Alternaria alternata) and pollens, Bet v 1 (Birch, Betula verrucosa) and Phl p 5 (Timothy grass, Phleum pratense), Ara h 6 and Ara h 3 (Arachis hypogaea). This kit may be used for analysis of the above indoor allergens in environmental samples, such as house dust extracts or air filter samples and other biologic or environmental samples. 10. Assay Performance 2. Reagent Lots Supplied MARIA Multiplex Array for Indoor Allergens Cat# MRA-C8 Lot# xxxxx Expiry is 12 months from ship date: Kit contents: Magnetic Microspheres: Standards: Der p 1 Lot# xxxxx ST-UAS Lot# xxxxx Der f 1 xxxxx ST-AA1 Mite Group 2 xxxxx ST-AF1 Fel d 1 xxxxx ST-BV1 Can f 1 xxxxx ST-PP5 Mus m 1 xxxxx ST-AH6 Rat n 1 xxxxx ST-AH3 Bla g 2 xxxxx Alt a 1 Biotinylated Detection Ab Lot# xxxxx Asp f 1 Streptavidin-Phycoerythrin xxxxx Bet v 1 Quality Control Low xxxxx Phl p 5 Quality Control High xxxxx Ara h 6 Multiscreen 96-well filter plate Ara h 3 MARIA Antibody Pairs Intra-Assay %CV Inter-Assay %CV Limit of Detection (ng/ml) Der p 1 10B9/5H Der f 1 6A8/4C Der p 2 1D8/7A Fel d 1 6F9/3E Can f 1 10D4/6E Mus m 1 pab α MM Rat n 1 RUP6/RUP Bla g 2 1F3/4C Alt a 1 2C10/3B Asp f 1 pab α AF1/4A Bet v 1 3B4/2E Phl p 5 1D11/Bo Ara h 6 3B8/3E Ara h 3 1E8/4G Intra and Inter-Assay %CV values based on exemplary data collected from internal Quality Control Samples (QC-MRA) analyzed by our ISO compliant laboratory. 4 13
5 9. Sample Curves 3. Storage Conditions Upon Receipt If MARIA kits are used within 7 days of receipt the entire kit contents should be stored at 2-8 C. If MARIA kits are to be stored for more than 7 days, Standards and Quality Controls Low/High should be stored at 20 C (±5 C) and the remaining kit contents should be left at 2-8 C (±5 C). DO NOT FREEZE antibody-coupled fluorescent microspheres, biotinylated detector antibodies or Streptavidin- Phycoerythrin. 4. Materials Required but Not Provided 4.1 Reagents 1. Multiplex assay buffer ( sterile filtered 1% BSA-PBS-0.02% Tween 20, ph 7.4). Buffer recipe can be found on our web site: 2. Luminex Sheath Fluid (Luminex Catalog # , BioRad Catalog# ) 4.2 Instrumentation/Materials 1. Adjustable Pipettes with Tips (10 µl µl ) 2. Multichannel Pipettes (5 µl - 50 µl and 25 µl µl) 3. Reagent Reservoirs 4. Polypropylene Microcentrifuge Tubes 5. Aluminum Foil or Drawer (incubation in dark) 6. Absorbent Pads or Paper Towels 7. Laboratory Vortex 8. Vacuum Filtration Unit (Millipore Vacuum Manifold, Catalog # MAVM0960R) 9. Luminex xmap Instrument 12 5
6 5. Technical Notes The MARIA kit operator should carefully read the entire product insert before performing the assay and be sure to follow the recommended protocol in order to collect reliable and reproducible results. The MARIA Assay Buffer requires sterile filtration. Unfiltered assay buffer has a high particle load that will interfere with measurement in the xmap system. It will cause high bead aggregation ratios and may increase the time it takes to read the plate by 3 to 4 fold. It is important to centrifuge dust sample extracts before preparing sample dilutions in order to minimize the number of foreign particles that can cause needle blockages during instrument reading. DO NOT INVERT PLATE at any time throughout the assay. The vacuum manifold setting should be adjusted to allow well contents to drain slowly and still enable the plate to be easily removed from the manifold while running. The MARIA plate should be protected from light during all incubation steps to prevent photo-bleaching of the antibody-coupled florescent microspheres. Always ensure that the instrument needle is routinely cleaned to prevent clogs during plate reading. It is recommended that the MARIA plate be read on the instrument on the same day the assay is performed. Note: Microspheres should be re-suspended immediately before read. Instrument settings: calibrate on low PMT, set sample size to 50µl and read 50 beads per analyte and set gate to 4,000 to 10,000. Calculate results based on a 5 parameter logistic curve fit. For data analysis instructions see the provided protocol on our web site: Instructions.pdf For additional Frequently Asked Questions (FAQ) visit our support page: 7. MARIA Protocol (cont.) 7.15 Dilute Streptavidin-Phycoerythrin (pink cap) in a pipette basin by adding 50µL to 12mL of assay buffer. Remove plate contents by vacuum filtration and wash wells 2x with 100µL assay buffer while vacuum filtering between washes Add 100 µl diluted Streptavidin-Phycoerythrin to each well and mix vigorously by pipetting while changing tips between plate columns Incubate for 30 minutes at room temperature in the dark.* *During this incubation period, prepare the instrument for plate reading according to the manufacturer s instructions. See also 5. Technical Notes Remove plate contents by vacuum filtration and wash wells 2x with 100μL of assay buffer while vacuum filtering between washes. Tap the bottom of the plate on paper towels to remove any excess buffer Add 100μL of assay buffer to all wells and resuspend the microspheres by pipetting repeatedly while changing tips between plate columns, taking care not to create bubbles Read the plate on the Luminex 100/200 instrument. 8. MARIA 96 Well Plate Layout 6 11
7 7. MARIA Protocol (cont.) 6. Certificate of Analysis Preparation of Samples 7.6 Vortex samples vigorously for 30 seconds and then centrifuge at 14,000 rpm (16,000 x g) for two minutes. We recommend preparing the following sample dilutions using assay buffer in a separate 96 well plate or microcentrifuge tubes: House dust extracts: 1/10, 1/100 and 1/10,000 Air filter extracts: undiluted, 1/5 and 1/20. Quality Control Samples (Product Code: QC-MRA) (optional): undiluted Immunoassay Protocol 7.7 Remove buffer from the 96 well filter plate by vacuum filtration. Tap the bottom of the plate on paper towels to remove excess buffer to prevent filter wicking. Repeat vacuum filtration. Tap plate again on paper towels. *Do Not Invert Plate* 7.8 Vortex the prepared microsphere solution for 30 seconds and pour entire contents into a pipette basin. Use a multichannel pipette to add 50 μl of microsphere solution to each well. 7.9 Add 50 μl of either diluted standards in duplicate wells, sample dilutions or assay buffer (blanks) to the appropriate wells. See MARIA 96 Well Plate Layout for a recommendation Set a multichannel pipette to 50μL and mix all wells vigorously (5-10 repetitions) while changing tips between plate columns. *Note: foam or bubbles may occur when mixing 7.11 Incubate for one hour at room temperature in the dark Dilute the Biotinylated Detector Antibody Mix (amber cap) in a pipette basin by adding 96µL to 12mL of assay buffer and mix thoroughly. Remove entire plate contents by vacuum filtration and wash wells 2x with 100 µl assay buffer while vacuum filtering between washes Add 100µl diluted Biotinylated Detector Antibody Mix to each well and mix vigorously by pipetting while changing tips between plate columns Incubate for one hour at room temperature in the dark. Microsphere Details: Antibody-coupled fluorescent microspheres are supplied individually: Analyte Product Code Bead Region Antibody Der p 1 MS-DP B9 Der f 1 MS-DF1 51 6A8 Mite Group 2 MS-MG2 53 1D8 Fel d 1 MS-FD1 58 6F9 Can f 1 MS-CF D4 Mus m 1 MS-MM1 62 pab α Mus m 1* Rat n 1 MS-RN1 69 RUP-6 Bla g 2 MS-BG2 47 1F3 Alt a 1 MS-AA1 28 2C10 Asp f 1 MS-AF1 22 pab α Asp f 1* Bet v 1 MS-BV1 39 3B4 Phl p 5 MS-PP5 43 1D11 Ara h 6 MS-AH6 18 3B8 Ara h 3 MS-AH3 1 1E8 * Polyclonal antibody Biotinylated Detector Antibody Details: Biotinylated detector antibodies are supplied premixed: Analyte *Polyclonal antibody Antibody Der p 1 5H8 Der f 1 4C1 Mite Group 2 7A1 Fel d 1 3E4 Can f 1 6E9 Mus m 1 pab α Mus m 1* Rat n 1 RUP-1 Bla g 2 4C3 Alt a 1 3B6 Asp f 1 4A6 Bet v 1 2E10 Phl p 5 Bo1 Ara h 6 3E12 Ara h 3 4G9 Biotinylation: Biotinylated using EZ-Link Sulfo-NHS-LC Biotinylating Agent and titrated for use in the array. Prepared in 1% BSA/50% glycerol/pbs, 0.22µm filtered, preservative free. 10 7
8 Allergen Standards Details: The Universal Allergen Standard (Cat# ST-UAS) is a formulation of eight purified natural allergens prepared in 1% BSA/50% glycerol/pbs, ph 7.4. Individual Allergen Standards (Cat# ST-AR1, ST-AH6, ST-AH3) are purified natural allergens prepared in 1% BSA/50% glycerol/pbs, ph 7.4. Individual Allergen Standards (Cat# ST-AA1, ST-BV1, ST-PP5) are purified recombinant allergens prepared in 1% BSA/50% glycerol/pbs, ph 7.4. Concentration/Calibration: 6. Certificate of Analysis (cont.) 7. MARIA Protocol 7.1 Remove dust/air filter extracts for analysis and QC samples (if applicable) from freezer and allow to reach room temperature. 7.2 Pre-wet each well of the 96 well filter plate with 100 μl of MARIA assay buffer. *Tip: When pipetting into the 96 well filter plate, insert the pipette tip at an angle into the bottom corner of the well. This will help ensure that the tip does not puncture the filter. Preparation of Microsphere Solution 7.3 Add 5.5 ml of assay buffer to a tube and label the tube Bead Mix. From the Bead Mix tube pipette 100µl of assay buffer into each vial of microspheres provided (blue caps). Vortex each vial of microspheres for one minute and then quick spin each vial for one second. Pipette the entire contents of each vial back into the labeled Bead Mix tube. Mix well by vortexing. Store in the dark while preparing standards and samples. Allergen Standard Product Code Protein Measurement Concentration (ng/ml) The bead set assignments are as listed on page 7 under the microsphere details table. Der p 1 Der f 1 Der p 2 Fel d 1 Can f 1 Mus m 1 Rat n 1 Bla g 2 Alt a 1 ST-UAS Amino-acid analysis 2500 Amino-acid analysis 2500 Amino-acid analysis 1000 Amino-acid analysis 1000 Amino-acid analysis 2500 Amino-acid analysis 250 Amino-acid analysis 1000 Amino-acid analysis 2500 ST-AA1 Amino-acid analysis 1000 Asp f 1 ST-AR1 Amino-acid analysis 400 Bet v 1 Phl p 5 ST-BV1 Advanced Protein Assay 1000 ST-PP5 Advanced Protein Assay 5000 Ara h 6 ST-AH6 Amino-acid analysis 1000 Ara h 3 ST-AH3 Amino-acid analysis 1250 Steptavidin-Phycoerythrin: Streptavidin, R-Phcoerythrin Conjugate (SAPE) is a biotin-binding protein used to measure fluorescence intensity in MARIA. Preparation of Allergen Standard 7.4 Prepare the allergen standard (yellow caps) starting dilution according to the allergens to be analyzed: 15µl ST-UAS, 15µl ST-AA1, 30µl ST-AR1, 30µl ST-BV1, 6µl ST-PP5, 15µl ST-AH6, 30µl ST-AH3. Bring the final volume to 300µl with assay buffer. Mix well and label tube 1. Example 1: When analyzing an 11-plex, add 15µl ST-UAS, 15µl ST-AA1, 30µl ST-BV1, 6µl ST-PP5 to 234µl assay buffer. Example 2: When analyzing a 2-plex (BV1, PP5), add 30µl ST-BV1, 6µl ST-PP5 to 264µl assay buffer. 7.5 Label eleven microcentrifuge tubes 2-12 and add 150µl of assay buffer to each of the tubes. Prepare the remainder of the standard curve using doubling dilutions of the allergen standard preparation from tube 1: Pipette 150μl allergen standard from tube 1 into 150 μl assay buffer into tube 2, mix well. Continue to make a total of 12 standard curve points. * Tip: To ensure accuracy, it is important to mix reagents containing glycerol thoroughly before and during dilutions* The 12-point standard curve ranges: ng/ml for Der p 1, Der f 1, Can f 1, Bla g 2 and Ara h ng/ml for Bet v 1 and Phl p ng/ml for Mite Group 2, Fel d 1, Rat n 1, Alt a 1, and Ara h ng/ml for Asp f ng/ml for Mus m 1 8 9
MARIA TM Premixed Multiplex Array for Indoor Allergens (8-Plex)
MARIA TM Premixed Multiplex Array for Indoor Allergens (8-Plex) Product Code: MRA-P8 Lot Number: xxxxx www.inbio.com Indoor Biotechnologies, Inc. 1216 Harris Street, Charlottesville Virginia, 2293 United
More informationMARIA TM Premixed Multiplex Array for Indoor Allergens (5-plex)
MARIA TM Premixed Multiplex Array for Indoor Allergens (5-plex) Product Code: MRA-P5B Lot Number: xxxxx www.inbio.com Indoor Biotechnologies, Inc. 1216 Harris Street, Charlottesville Virginia, 2293 United
More informationMARIA PickYourPlex Multiplex Array for Indoor Allergens Kit 96 Well Plate Assay
MARIA PickYourPlex Multiplex Array for Indoor Allergens Kit 96 Well Plate Assay www.inbio.com Indoor Biotechnologies, Inc. 1216 Harris Street, Charlottesville Virginia, 22903 United States Tel: (434) 984-2304
More informationMARIA PickYourPlex with Magnetic Beads Multiplex Array for Indoor Allergens Kit 96 Well Plate Assay
MARIA PickYourPlex with Magnetic Beads Multiplex Array for Indoor Allergens Kit 96 Well Plate Assay www.inbio.com Indoor Biotechnologies, Inc. 700 Harris Street, Charlottesville, VA 22903 United States
More informationNew Formulation of Amb a 1 ELISA 2.0 and MARIA
Stephanie Filep Director of Laboratory Services 700 Harris Street Charlottesville VA 22903 U.S.A. TEL: (1) (434) 984 2304 FAX: (1) (434) 984 2709 www.inbio.com March 23, 2018 New Formulation of Amb a 1
More informationGSI Canine IL-5 ELISA Kit-2 Plates DataSheet
Interleukin5 (IL5) is a secreted glycoprotein that belongs to the α-helical group of cytokines (1, 2, 3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). IL-5
More informationCONTENTS. STUDY DESIGN METHODS ELISA protocol for quantitation of mite (Dermatophagoides spp.) Der p 1 or Der f 1
CONTENTS STUDY DESIGN METHODS ELISA protocol for quantitation of mite (Dermatophagoides spp.) Der p 1 or Der f 1 ELISA protocol for mite (Dermatophagoides spp.) Group 2 ALLERGENS RESULTS (SUMMARY) TABLE
More informationRayBio Human Granzyme B ELISA Kit
RayBio Human Granzyme B ELISA Kit Catalog #: ELH-GZMB User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,
More informationNori Rabbit IL-2 ELISA Kit DataSheet
Nori Rabbit IL-2 ELISA Kit DataSheet IL-2 is an interleukin, a type of cytokine immune system signaling molecule. IL-2 is a T cell stimulatory cytokine best known for inducing T cell proliferation, the
More informationMouse C-Peptide ELISA Kit
Mouse C-Peptide ELISA Kit Cat.No: DEIA4507 Lot. No. (See product label) Size 96T Intended Use The Mouse C-Peptide ELISA kit is for the quantitative determination of c-peptide in mouse serum, plasma, and
More information02006B 1 vial 02006B 1 vial Store at -20 C. Lyophilized recombinant IL-2
For detection and measurement of human interleukin 2 Catalog #02006 Catalog #02007 2 Plates 10 Plates Product Description The Human Interleukin 2 (IL-2) ELISA Kit is designed for the quantitative detection
More informationWideScreen Human Metabolism Panel 2
User Protocol TB531 Rev. C 0710JN Page 1 of 10 WideScreen Human Metabolism Panel 2 Table of Contents About the Kit... 2 Overview 2 Components and Storage... 4 Additional Materials Required But Not Supplied
More informationhuman Total Cathepsin B Catalog Number: DY2176
human Total Cathepsin B Catalog Number: DY2176 This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Total
More informationHuman HIV (1+2) antigen&antibody ELISA Kit
Human HIV (1+2) antigen&antibody ELISA Kit Catalog Number. CSB-E18042h For the qualitative determination of human HIV (1+2) antibody and P24 antigen concentrations in serum, plasma. This package insert
More informationRayBio Human, Mouse and Rat Phospho-NF-kB P65 (Ser536) and Total NF-kB P65 ELISA Kit
RayBio Human, Mouse and Rat Phospho-NF-kB P65 (Ser536) and Total NF-kB P65 ELISA Kit Catalog #: PEL-NFKBP65-S536-T User Manual Last revised October 10, 2017 Caution: Extraordinarily useful information
More informationRat Insulin ELISA. For the quantitative determination of insulin in rat serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures.
Rat Insulin ELISA For the quantitative determination of insulin in rat serum and plasma For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: Size: 80-INSRT-E01, E10 96 wells, 10
More informationBlood Urea Nitrogen Enzymatic Kit Manual Catalog #:
BIOO LIFE SCIENCE PRODUCTS Blood Urea Nitrogen Enzymatic Kit Manual Catalog #: 5602-01 BIOO Scientific Corp. 2010 TABLE OF CONTENTS GENERAL INFORMATION... 1 Product Description... 1 Procedure Overview...
More informationMagniSort Mouse CD4 Naive T cell Enrichment Kit Catalog Number: RUO: For Research Use Only. Not for use in diagnostic procedures.
Page 1 of 2 MagniSort Mouse CD4 Naive T cell Enrichment Kit RUO: For Research Use Only. Not for use in diagnostic procedures. Mouse splenocytes were unsorted (left) or sorted with the MagniSort Mouse CD4
More informationBlood Urea Nitrogen Enzymatic Kit Manual Catalog #:
Blood Urea Nitrogen Enzymatic Kit Manual Catalog #: 5602-01 TABLE OF CONTENTS GENERAL INFORMATION... 2 Product Description... 2 Procedure Overview... 2 Kit Contents, Storage and Shelf Life... 3 Required
More informationab Exosome Isolation and Analysis Kit - Flow Cytometry, Cell Culture (CD63 / CD81)
Version 1 Last updated 26 September 2018 ab239682 Exosome Isolation and Analysis Kit - Flow Cytometry, Cell Culture (CD63 / For the isolation and analysis of exosome from cell culture. This product is
More informationSTAT3 (py705)/ Pan STAT3 (Human/Mouse/Rat) ELISA Kit
STAT3 (py705)/ Pan STAT3 (Human/Mouse/Rat) ELISA Kit Catalog Number KA2176 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Principle of the Assay...
More informationRayBio Human ENA-78 ELISA Kit
RayBio Human ENA-78 ELISA Kit Catalog #: ELH-ENA78 User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross,
More informationInstructions for use. TSH rat ELISA. Please use only the valid version of the Instructions for Use provided with the kit AR E-8600
Instructions for use TSH rat ELISA AR E-8600 TSH rat ELISA 1. INTRODUCTION 1.1 Intended use The TSH rat ELISA is an enzyme immunoassay for the quantitative measurement of TSH in rat serum. For research
More informationPorcine/Canine Insulin ELISA
Porcine/Canine Insulin ELISA For the quantitative determination of insulin in porcine or canine serum and plasma. Please read carefully due to Critical Changes, e.g., Calculation of Results. For Research
More informationConcentration Estimation from Flow Cytometry Exosome Data Protocol
Concentration Estimation from Flow Cytometry Exosome Data Protocol 1. STANDARD CURVE Create a standard curve for the target exosome by plotting the mean fluorescence (y axis) against the protein concentration
More informationHuman Obestatin ELISA
K-ASSAY Human Obestatin ELISA For the quantitative determination of obestatin in human serum and plasma Cat. No. KT-495 For Research Use Only. 1 Rev. 081309 K-ASSAY PRODUCT INFORMATION Human Obestatin
More informationRayBio Human Phosphotyrosine BTK ELISA Kit
RayBio Human Phosphotyrosine BTK ELISA Kit Catalog #: PEL-BTK-Y User Manual Last revised August 10, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite
More informationHuman Apolipoprotein A1 EIA Kit
A helping hand for your research Product Manual Human Apolipoprotein A1 EIA Kit Catalog Number: 83901 96 assays 1 Table of Content Product Description 3 Assay Principle 3 Kit Components 3 Storage 4 Reagent
More informationBovine Insulin ELISA
Bovine Insulin ELISA For quantitative determination of insulin in bovine serum and plasma. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 80-INSBO-E01 Size: 96 wells Version:
More informationMagniSort Human CD4 T cell Enrichment Kit Catalog Number: RUO: For Research Use Only. Not for use in diagnostic procedures.
Page 1 of 2 MagniSort Human CD4 T cell Enrichment Kit RUO: For Research Use Only. Not for use in diagnostic procedures. Normal human peripheral blood mononuclear cells were unsorted (left) or sorted with
More informationab Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture
Version 1 Last updated 14 March 2018 ab228564 Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture For the measurement of human exosomes in cell culture. This product is for research use only
More informationSTAT1 (ps727) (Human/Mouse) ELISA Kit
STAT1 (ps727) (Human/Mouse) ELISA Kit Catalog Number KA2171 96 assays Version: 01 Intended for research use only www.abnova.com I. INTRODUCTION STAT1 (ps727) (Human/Mouse) ELISA (Enzyme-Linked Immunosorbent
More informationEliKine Free Thyroxine (ft4) ELISA Kit
EliKine Free Thyroxine (ft4) ELISA Kit Booklet Item NO. KET0005 Product Name EliKine Free Thyroxine (ft4) ELISA Kit ATTENTION For laboratory research use only. Not for clinical or diagnostic use TABLE
More informationMagniSort Mouse CD4 T cell Enrichment Kit Catalog Number: RUO: For Research Use Only. Not for use in diagnostic procedures.
Page 1 of 2 MagniSort Mouse CD4 T cell Enrichment Kit RUO: For Research Use Only. Not for use in diagnostic procedures. Mouse splenocytes were unsorted (left) or sorted with the MagniSort Mouse CD4 T cell
More informationGladstone Institutes, University of California (UCSF), San Francisco, USA
Fluorescence-linked Antigen Quantification (FLAQ) Assay for Fast Quantification of HIV-1 p24 Gag Marianne Gesner, Mekhala Maiti, Robert Grant and Marielle Cavrois * Gladstone Institutes, University of
More informationHIV-1 p24 ELISA Kit. Catalog Number KA assays Version: 06. Intended for research use only.
HIV-1 p24 ELISA Kit Catalog Number KA0452 96 assays Version: 06 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Principle of the Assay... 3 General Information... 4 Materials
More informationMagniSort Human CD4 Memory T cell Enrichment Kit Catalog Number: RUO: For Research Use Only. Not for use in diagnostic procedures.
Page 1 of 2 MagniSort Human CD4 Memory T cell Enrichment Kit RUO: For Research Use Only. Not for use in diagnostic procedures. Normal human peripheral blood mononuclear cells were unsorted (left) or sorted
More informationMouse Ultrasensitive Insulin ELISA
Mouse Ultrasensitive Insulin ELISA For the quantitative determination of insulin in mouse serum and plasma. Please read carefully due to Critical Changes, e.g., Calculation of Results. For Research Use
More informationData Sheet. CD28:B7-2[Biotinylated] Inhibitor Screening Assay Kit Catalog # Size: 96 reactions
Data Sheet CD28:B7-2[Biotinylated] Inhibitor Screening Assay Kit Catalog # 72062 Size: 96 reactions BACKGROUND: The activation of naïve T cells requires two signals; the specific T cell receptor recognition
More informationHuman Oxidized LDL ELISA Kit (MDA-LDL Quantitation), General
Human Oxidized LDL ELISA Kit (MDA-LDL Quantitation), General For the detection and quantitation of human OxLDL in plasma, serum or other biological fluid samples Cat. No. KT-959 For Research Use Only.
More informationMouse C-peptide ELISA
Mouse C-peptide ELISA For the quantitative determination of C-peptide in mouse serum. For Research Use Only. Not for use in Diagnostic Procedures. Please read carefully due to Critical Changes, e.g., Calculation
More informationRayBio Human Thyroglobulin ELISA Kit
RayBio Human Thyroglobulin ELISA Kit Catalog #: ELH-Thyroglobulin User Manual Last revised July 6, 2017 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite
More informationRat cholesterol ELISA Kit
Rat cholesterol ELISA Kit Catalog No. CSB-E11706r (96T) This immunoassay kit allows for the in vitro quantitative determination of rat Cholesterol concentrations in serum, plasma and other biological fluids.
More informationElectrolytes. Summary: (This area will include a brief description of what the protocol is used for and why someone would need to use it.
Electrolytes Version: 1 Edited by: Jason Kim (note that the following list should be linked to the appropriate location.) Summary Reagents and Materials Protocol Reagent Preparation Reagent 1 Reagent 2
More informationEXOTESTTM. ELISA assay for exosome capture, quantification and characterization from cell culture supernatants and biological fluids
DATA SHEET EXOTESTTM ELISA assay for exosome capture, quantification and characterization from cell culture supernatants and biological fluids INTRODUCTION Exosomes are small endosome-derived lipid nanoparticles
More informationRayBio Human Phospho-DDR2 (Tyr740) and Total DDR2 ELISA Kit
RayBio Human Phospho-DDR2 (Tyr740) and Total DDR2 ELISA Kit Catalog #: PEL-DDR2-Y740-T User Manual Last revised March 22, 2018 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607
More informationHbA1c (Human) ELISA Kit
HbA1c (Human) ELISA Kit Cat. No.:DEIA3509 Pkg.Size:96T Intended use GHbA1c (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human GHbA1c. General Description vhemoglobin,
More informationPRODUCT INFORMATION & MANUAL
PRODUCT INFORMATION & MANUAL Overall Exosome Capture and Quantification (Plasma, Colorimetric) Kit NBP2-49782 For research use only. Not for diagnostic or therapeutic procedures. www.novusbio.com - P:
More informationMouse C-peptide EIA. Cat. No. YII-YK013-EX FOR LABORATORY USE ONLY
Mouse C-peptide EIA Cat. No. YII-YK013-EX FOR LABORATORY USE ONLY TOYO 2CHOME, KOTO-KU, TOKYO, 135-0016, JAPAN http://www.cosmobio.co.jp e-mail : export@cosmobio.co.jp Phone : +81-3-5632-9617 FAX : +81-3-5632-9618
More informationab Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma
Version 1 Last updated 25 May 2018 ab228565 Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma For the isolation/detection of exosomes from human plasma, urine or cell culture media. This product
More informationRat C-peptide ELISA. For the quantitative determination of C-peptide in rat serum
Rat C-peptide ELISA For the quantitative determination of C-peptide in rat serum Please read carefully due to Critical Changes, e.g., see Calculation of Results. For Research Use Only. Not For Use In Diagnostic
More informationMouse C-peptide ELISA
Mouse C-peptide ELISA For the quantitative determination of C-peptide in mouse serum. For Research Use Only. Not for use in Diagnostic Procedures. Please read carefully due to Critical Changes, e.g., Preparation
More informationHuman TSH ELISA Kit. User Manual
Human TSH ELISA Kit User Manual Catalog number: GTX15585 GeneTex Table of Contents A. Product Description... 2 B. Kit Components... 3 C. Additional Required Materials (not included)... 3 D. Reagent Preparation...
More informationMouse Cathepsin B ELISA Kit
GenWay Biotech, Inc. 6777 Nancy Ridge Drive San Diego, CA 92121 Phone: 858.458.0866 Fax: 858.458.0833 Email: techline@genwaybio.com http://www.genwaybio.com Mouse Cathepsin B ELISA Kit Catalog No. GWB-ZZD154
More informationRayBio Human Phospho-DDR1 (Tyr792) and Total DDR1 ELISA Kit
RayBio Human Phospho-DDR1 (Tyr792) and Total DDR1 ELISA Kit Catalog #: PEL-DDR1-Y792-T User Manual Last revised March 22, 2018 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607
More informationSerum Amyloid A ELISA
Serum Amyloid A ELISA For the quantitative determination of serum amyloid A (SAA) in serum plasma For Research use Only. Not for Use in Diagnostic Procedures Please see Appendix A for Reference Serum information
More informationSTAT3 (py705) (Human/Mouse/Rat) ELISA Kit
STAT3 (py705) (Human/Mouse/Rat) ELISA Kit Catalog Number KA2175 96 assays Version: 01 Intended for research use only www.abnova.com I. INTRODUCTION STAT3 (py705) (Human/Mouse/Rat) ELISA (Enzyme-Linked
More informationRat Proinsulin ELISA
Rat Proinsulin ELISA For the quantitative determination of proinsulin in rat serum For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 80-PINRT-E01 Size: 96 wells Version: June
More informationMouse Leptin ELISA Kit Instructions
V.6/Mar/2008 CRYSTAL CHEM INC. Mouse Leptin ELISA Kit Instructions For the quantitative determination of leptin in mouse serum or plasma and fluid Catalog #: 90030 96 Assays For Research Use Only. Not
More informationHuman Cathepsin D ELISA Kit
GenWay Biotech, Inc. 6777 Nancy Ridge Drive San Diego, CA 92121 Phone: 858.458.0866 Fax: 858.458.0833 Email: techline@genwaybio.com http://www.genwaybio.com Human Cathepsin D ELISA Kit Catalog No. GWB-J4JVV9
More informationInsulin (Porcine/Canine) ELISA
Insulin (Porcine/Canine) ELISA For the quantitative measurement of insulin in Porcine/Canine serum and plasma (EDTA) For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 80-INSPO-E01
More information155 S. Milpitas Blvd., Milpitas, CA USA T: (408) F: (408)
ExoQuant TM Tumor-derived Exosome enrichment and Quantification Assay Kit (biological fluids & cell media, luminometric) (Catalog # K129-1; 1 assays (96 well transparent immunoplate for luminometric readings;
More informationGLP-2 (Rat) ELISA. For the quantitative determination of glucagon-like peptide 2 (GLP-2) in rat serum and plasma
GLP-2 (Rat) ELISA For the quantitative determination of glucagon-like peptide 2 (GLP-2) in rat serum and plasma For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: 48-GP2RT-E01
More informationCOMPONENT NAME COMPONENT # QUANTITY STORAGE SHELF LIFE FORMAT. Store at 2-8 C. Do not freeze. Store at 2-8 C. Do not freeze.
This document is available at www.stemcell.com/pis Catalog #18765 EasySep Mouse CD4+CD62L+ T Cell Isolation Kit For processing 1x 10^9 cells Description Isolate highly purified naïve CD4+ T cells (CD4+CD62L+)
More informationAPOB (Human) ELISA Kit
APOB (Human) ELISA Kit Catalog Number KA4330 96 assays Version: 01 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 Principle of the Assay...
More informationData Sheet. PCSK9[Biotinylated]-LDLR Binding Assay Kit Catalog # 72002
Data Sheet PCSK9[Biotinylated]-LDLR Binding Assay Kit Catalog # 72002 DESCRIPTION: The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. PCSK9 is known to function
More informationHuman LDL ELISA Kit. Innovative Research, Inc.
Human LDL ELISA Kit Catalog No: IRKTAH2582 Lot No: SAMPLE INTRODUCTION Human low-density lipoprotein (LDL) transports cholesterol from the liver to tissues where it is incorporated into cell membranes.
More informationSIV p27 Antigen ELISA Catalog Number:
INTENDED USE The RETRO-TEK SIV p27 Antigen ELISA is for research use only and is not intended for in vitro diagnostic use. The RETRO-TEK SIV p27 Antigen ELISA is an enzyme linked immunoassay used to detect
More informationHuman IL-2 ELISA Antibody Pair
Human IL-2 ELISA Antibody Pair For the qualitative and quantitative determination of IL-2 in serum, plasma and cell culture. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number:
More informationRayBio Human Phospho-DDR1 (Tyr792) ELISA Kit
RayBio Human Phospho-DDR1 (Tyr792) ELISA Kit Catalog #: PEL-DDR1-Y792 User Manual Last revised March 22, 2018 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane,
More informationTECHNICAL BULLETIN. C-Peptide EIA Kit for serum, plasma, culture supernatant, and cell lysates. Catalog Number RAB0326 Storage Temperature 20 C
C-Peptide EIA Kit for serum, plasma, culture supernatant, and cell lysates Catalog Number RAB0326 Storage Temperature 20 C TECHNICAL BULLETIN Product Description The C-Peptide Enzyme Immunoassay (EIA)
More informationFOR LABORATORY USE ONLY
YK060 Insulin ELISA FOR LABORATORY USE ONLY Kasumigaseki Place, 3-6-7, Kasumigaseki, Chiyoda-ku Tokyo 100-0013 Japan URL: http://www.sceti.co.jp/export/ e-mail: medical@sceti.co.jp Contents
More informationHuman LDL Receptor / LDLR ELISA Pair Set
Human LDL Receptor / LDLR ELISA Pair Set Catalog Number : SEK10231 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized in
More informationMouse C3 (Complement Factor 3) ELISA Kit
Mouse C3 (Complement Factor 3) ELISA Kit Cat. No.:DEIA8289 Pkg.Size:96T Intended use The Mouse C3 (Complement Factor 3) ELISA Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring
More informationab65344 Uric Acid Assay Kit (Colorimetric/Fluorometric)
ab65344 Uric Acid Assay Kit (Colorimetric/Fluorometric) Instructions for Use For the rapid, sensitive and accurate measurement of uric acid in various samples. This product is for research use only and
More informationRat C-peptide ELISA. For the quantitative determination of C-peptide in rat serum. For Research Use Only. Not For Use In Diagnostic Procedures.
Rat C-peptide ELISA For the quantitative determination of C-peptide in rat serum. For Research Use Only. Not For Use In Diagnostic Procedures. Catalog Number: Size: 80-CPTRT-E01 96 wells Version: May 26,
More informationProthrombin (Human) ELISA Kit
Prothrombin (Human) ELISA Kit Catalog Number KA0496 96 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General
More informationPYY (Human/Mouse/Rat) ELISA Kit
PYY (Human/Mouse/Rat) ELISA Kit Catalog Number KA1686 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3
More informationInstructions for use. TSH rat ELISA. Please use only the valid version of the Instructions for Use provided with the kit AR E-8600
Instructions for use TSH rat ELISA AR E8600 TSH rat ELISA INTRODUCTION INTENDED USE The TSH rat ELISA is an enzyme immunoassay for the quantitative measurement of TSH in rat serum. For research use only.
More informationInsulin ELISA. For the quantitative determination of insulin in serum and plasma.
Insulin ELISA For the quantitative determination of insulin in serum and plasma. For In Vitro Diagnostic use within the United States of America. This product is for Research Use Only outside of the United
More informationEGFR (py1045)/ Pan EGFR (Human) ELISA Kit
EGFR (py1045)/ Pan EGFR (Human) ELISA Kit Catalog Number KA2156 96 assays Version: 01 Intended for research use only www.abnova.com I. INTRODUCTION EGFR (py1045)/pan EGFR (Human) ELISA (Enzyme-Linked Immunosorbent
More informationHuman Leptin ELISA Kit
Product Manual Human Leptin ELISA Kit Catalog Numbers MET-5057 MET-5057-5 96 assays 5 x 96 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Leptin is a polypeptide hormone
More informationExosome ELISA Complete Kits
Exosome ELISA Complete Kits EXOEL-CD9A-1, EXOEL-CD63A-1, EXOEL-CD81A-1 User Manual See PAC for Storage Conditions for Individual Components Version 12 4/17/2017 A limited-use label license covers this
More informationVantage Diabetes Panel 1
Catalog No. AM100050 50 Reactions To be used in conjunction with the Vantage microrna Detection Kit, catalog number AM100091 (50 reactions) or AM100092 (200 reactions). Components included in this kit:
More informationInfluenza A H1N1 HA ELISA Pair Set
Influenza A H1N1 HA ELISA Pair Set for H1N1 ( A/Puerto Rico/8/1934 ) HA Catalog Number : SEK11684 To achieve the best assay results, this manual must be read carefully before using this product and the
More informationYK052 Mouse Leptin ELISA
YK052 Mouse Leptin ELISA FOR LABORATORY USE ONLY YANAIHARA INSTITUTE INC. 2480-1 AWAKURA, FUJINOMIYA-SHI SHIZUOKA, JAPAN 418-0011 Contents Ⅰ. Introduction 2 Ⅱ. Characteristics 3 Ⅲ. Composition 4 Ⅳ. Method
More informationRayBio Human Allergen Protein Array G2
RayBio Human Allergen Protein Array G2 Glass-chip Based Allergen Protein Array User Manual (Revised Jul 26, 2017) For simultaneous detection of human IgE and IgG associated with 22 common allergens in
More informationMouse TrkB ELISA Kit
Mouse TrkB ELISA Kit CATALOG NO: IRKTAH5472 LOT NO: SAMPLE INTENDED USE For quantitative detection of mouse TrkB in cell culture supernates, cell lysates and tissue homogenates. BACKGROUND TrkB receptor
More informationab Serum Amyloid P (APCS) Human ELISA Kit
ab137970 Serum Amyloid P (APCS) Human ELISA Kit Instructions for Use For the quantitative measurement of Human Serum Amyloid P (APCS) in plasma, serum, urine, and cell culture supernatants. This product
More informationRayBio Human/Mouse/Rat GIP Enzyme Immunoassay Kit
RayBio Human/Mouse/Rat GIP Enzyme Immunoassay Kit Please Read the Manual Carefully Before Starting your Experiment User Manual 2.2 (Revised March 15, 2012) RayBio GIP Enzyme Immunoassay Kit Protocol (Cat#:
More informationCOMPONENT NAME COMPONENT # QUANTITY STORAGE SHELF LIFE FORMAT. Store at 2-8 C. Do not freeze. Store at 2-8 C. Do not freeze.
This document is available at www.stemcell.com/pis Catalog #18765 EasySep Mouse CD4+CD62L+ T Cell Isolation Kit For processing 1x 10^9 cells Description Isolate highly purified naïve CD4+ T cells (CD4+CD62L+)
More informationInfluenza B Hemagglutinin / HA ELISA Pair Set
Influenza B Hemagglutinin / HA ELISA Pair Set Catalog Number : SEK11053 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized
More informationTECHNICAL BULLETIN. Catalog Number RAB0447 Storage Temperature 20 C
Phospho-Stat3 (ptyr 705 ) and pan-stat3 ELISA Kit for detection of human, mouse, or rat phospho-stat3 (ptyr 705 ) and pan-stat3 in cell and tissue lysates Catalog Number RAB0447 Storage Temperature 20
More informationab Thyroglobulin Human ELISA Kit
ab155441 Thyroglobulin Human ELISA Kit Instructions for Use For the quantitative measurement of Human Thyrogobulin in serum, plasma and cell culture supernatants. This product is for research use only
More informationExosome ELISA Complete Kits
Exosome ELISA Complete Kits EXOEL-CD9A-1, EXOEL-CD63A-1, EXOEL-CD81A-1 User Manual See PAC for Storage Conditions for Individual Components Version 12 4/17/2017 A limited-use label license covers this
More informationMorinaga Mouse C-peptide ELISA Kit
Morinaga Mouse C-peptide ELISA Kit For the quantitative determination of C-peptide in mouse serum, plasma, and fluid 96wells For Laboratory Use Only, not for use in diagnostic procedure Please read full
More informationHuman Immunodeficiency Virus type-1 p24 protein (HIV p24) Kit
TECHNICAL DATA SHEET AlphaLISA Research Reagents Caution: For Laboratory Use. A research chemical for research purposes only. Human Immunodeficiency Virus type-1 p24 protein (HIV p24) Kit Product No.:
More informationHuman IL-2. Pre-Coated ELISA Kit
Human IL-2 (Interleukin 2) Pre-Coated ELISA Kit Catalog No: 90-2083 1 96 well Format (96 tests) Detection Range: 31.2 2000 pg/ml Sensitivity: < 18.75 pg/ml This immunoassay kit allows for the in vitro
More informationEXOCET Exosome Quantitation Assay
EXOCET Exosome Quantitation Assay EXOCET96A-1 User Manual Check Package Contents for Storage Temperatures Version 3 5/30/2017 A limited-use label license covers this product. By use of this product, you
More information