RLB CIH, LLC. Final Report Microbial Contamination Sampling Taos Recreation Center Taos, New Mexico. March 9, 2007

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1 Final Report Microbial Contamination Sampling Taos Recreation Center Taos, New Mexico RLB CIH, LLC Ronald L. Beethe, CIH Alvarado NE FAX Albuquerque, NM March 9, 2007 The City of Taos, New Mexico has a recreation center which includes an enclosed pool area with a lap pool, therapy pool and play area with wading pool and water slide. There was a concern that the use of standard sheetrock in damp areas of the center, as well as poor floor drainage might be promoting mold growth within the facility. Ronald L. Beethe, CIH was contacted by Jim Thompson and requested to inspect the pool area, conduct an IR thermography survey, inspect wall cavities with a fiber optics borescope, and sample for fungal contamination. The inspection, sampling and survey were done on February 22, and included: Four microvac dust samples for fungal spores and culturable fungi Twelve WallChek sample for fungal spores Methods Infrared (IR) thermal imagery was conducted using a Fluke model Ti-31 thermal imager. Sheetrock wall and ceiling surfaces were surveyed for surface temperature. Reduced surface temperature in areas of the walls or ceilings could indicate elevated moisture. The fiber optics borescope inspection of wall cavities was conducted using a 48-inch, self illuminated borescope. The examinations were made through the same holes used for collecting the WallChek samples. Fungal spores and culturable fungi in dust were sampled using 25 millimeter diameter extended-cowl cassettes with membrane filters and a sample pump drawing approximately 30 lpm (analytical results are based on dust mass rather than volume of air sampled). Samples were collected from the: Ceiling vent in women s family change room Ceiling vent in men s shower Ceiling vent in men s restroom Ceiling vent over sink in men s family change room. The samples were sent to AeroTech P&K Laboratory for analysis. Fungal spores in a wall cavity were sampled using Air-O-Cell cassettes, the AeroTech WallChek sampling system, and an air sampling pump drawing 15 lpm. Sampling locations were specified by Mr. Thompson and were listed on building diagrams or maps. Samples were taken of the: Wall in women s family change room (map location # 9) Microbial Sampling Page 1 of 4

2 Microbial Contamination Sampling Taos Recreation Center, Taos, New Mexico RLB,CIH Door wall in women s family change room (map location #8) Therapy pool wall (map location #7) Wall outside humidity control room (map location #10) Pool to outdoors wall (East) (map location #11) Pool to outdoors wall (South) (map location #12) Men s room entry wall (map location #5) Men s family changing room wall (map location #6) Men s room hall wall (map location #4) Men s room restroom wall (map location #3) Men s room locker wall (map location #2) Men s room shower wall (map location #1) The samples were sent to AeroTech Laboratory for analysis.. Results and Discussion The infrared thermography survey detected thermal anomalies in: The women s family room ceiling above the door to the pool; The women s family room wall in the corner by a locker; The therapy pool area ceiling (marked with electrical tape); The humidity control room wall, above outlet (marked with electrical tape); The men s locker room ceiling near map location #4; and the men s locker room wall that backs on the showers. These areas may warrant further investigation for moisture intrusion. The fiber optics borescope inspection of wall cavities generally found no indications of moisture damage or mold, with shiny metal studs being visible in most walls inspected. However, there was a discolored (rusty or stained?) pipe in the wall between the men s shower room and locker room. This would seem to indicate that there is moisture intrusion into this wall cavity. The microscopic analyses of the microvac dust samples detected low spore concentrations in all the samples. A small amount of Stachybotrys, a fungi thought by some to be more hazardous, was detected in the sample from the women s family room vent. Microbiological analytical analyses of the dust samples detected low concentrations of culturable fungi in the dust (18 to 552 colony forming units [CFU] per sample). The most frequently detected fungal taxa were Acremonium and Stachybotrys. It is very unusual for Stachybotrys to be detected in culturable samples. The laboratory results and graphic plots of the results are attached. The WallChek samples for fungal spores from wall (or ceiling) cavities detected a wide range of fungal spore concentrations in the samples (0 to 221,367 spores/cubic meter). High spore concentrations were detected in the women s family changing room wall and the men s room entry wall. Aspergillus/Penicillium-like was the predominant fungal genera detected, but Stachybotrys was also found in both of these samples. The laboratory results and a graphic plot of the results are attached. Microbial Sampling Page 2 of 4

3 Microbial Contamination Sampling Taos Recreation Center, Taos, New Mexico RLB,CIH Overall, the inspection and sampling indicates that there is some significant fungal contamination within the portions of the building that were sampled, and that remedial actions are required. This is based on: The detection of Stachybotrys spores in all the dust samples The detection of Stachybotrys spores in some of the WallChek samples The detection of high concentrations of fungal spores (mostly Aspergillus/Penicillium - like) in the WallChek samples from the women s family changing room wall and the men s room entry wall The detection of culturable Stachybotrys fungi in all of the dust samples for culturable fungi The thermal anomalies observed may indicate potential problems, such as missing or shifted insulation, wet insulation separated from the drywall by a moisture barrier, or moisture in the sheetrock. Recommendations Open the lower 2 to 4 feet of the women s family changing room wall and the men s room entry wall to determine the source of the elevated fungal spores and to look for signs of moisture damage. Check the wall cavities above the open portions of the walls. Remove and replace any sheetrock (or other building materials) with visible fungal contamination or water damage. Because of the detection of Stachybotrys spores in the men s family change room wall, it might also be desirable to open this wall as well. HEPA vacuum any accessible wall cavities where there are no visual signs of fungal contamination or moisture damage. If the opened walls are made of standard sheetrock, replace this with moisture resistant sheetrock. Consider opening the walls or ceilings with thermal anomalies detected by the IR thermography survey to inspect for sources of moisture and visible moisture damage. The men s locker room/shower room wall should be opened to determined what is causing the pipe in the wall to be stained or rusted. Address any identified moisture sources. The remediation should be done by a qualified remediation or abatement company using techniques similar to those used for asbestos abatement (e.g.; critical barriers, negative air, personal protective equipment for workers). If such work is done improperly, the building could be significantly contaminated. Guidance from the EPA publication on mold remediation in schools and commercial building and AIHA Guideline Microbial Sampling Page 3 of 4

4 Microbial Contamination Sampling Taos Recreation Center, Taos, New Mexico RLB,CIH should be followed. After remediation consider sampling to verify that the remediation has successfully removed all significant fungal contamination. Thank you for the opportunity to provide professional industrial hygiene consulting services. Please contact us if you have any questions, or we can be of any further service. Sincerely yours, Ronald L. Beethe, CIH Microbial Sampling Page 4 of 4

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7 Taos Recreation Center WallChek Microscopic Analyses 02/22/07 250,000 Concentration (spores/cubic meter) 200, , ,000 Women's Family Changing Room Wall Door Wall in Women's Family Changing Room Therapy Pool Wall Wall Outside Humidity Control Room Pool to Outdoors Wall (E) Pool to Outdoors Wall (S) Men's Room Entry Wall Men's Family Changing Room Wall Men's Room Hall Wall Men's Room Restroom Wall Men's Room Locker Rm Wall Men's Room Shower Rm Wall 50,000 0 Total Fungal Spores Alternaria Aspergillus/ Penicillium- Like Basidiospores Chaetomium Cladosporium Stachybotrys Fungal Genera

8 Taos Recreation Center MicroVac Dust -- Microscopic Analyses 02/22/07 10,000 Women's Family Room Vent Men's Shower Vent Men's Restroom Vent Men's Family Room Over Sink 7,500 Concentration (spores/sample) 5,000 2,500 0 Total Fungal Spores Aspergillus/ Penicillium- Like Basidiospores Cladosporium Smuts/ Myxomycetes/ Periconia Fungal Genera Stachybotrys

9 Taos Recreation Center MicroVac Dust -- Microbiological Analyses 02/22/07 50,000 Women's Family Room Vent Men's Shower Vent Men's Restroom Vent Men's Family Room Over Sink 40,000 Concentration (CFU/sample) 30,000 20,000 10,000 0 Total Fungi Acremonium Aspergillus species Var. 1 Oidiodendron Fungal Genera or Species Penicillium species Var. 1 Stachybotrys Yeast

10 Beethe, Ronald L. CIH 400 Alvarado NE Albuquerque, NM Attn: Ronald Beethe Lab Number: AIHA EMLAP No Total Fungal Spore, Mycelia and Pollen Counts-Air Aerotech Method: WC001 Air-O-Cell Cassette Project Name: TP-0222 Date Received: 02/23/2007 Date Reported: 02/26/2007 Sample Number Sample Identification TPW-01 Ladies Family Chg Sn# TPW-02 Door Wall # TPW-03 Therapy Pool Wall # TPW-04 Ctrl Wall # Date Analyzed 2/23/2007 2/23/2007 2/23/2007 2/23/2007 Volume(M³) Percent Of Trace Analyzed 100% of Trace at 600x Magnification 100% of Trace at 600x Magnification 100% of Trace at 600x Magnification 100% of Trace at 600x Magnification Debris Rating Analyte Count/M³ Count/M³ Count/M³ Count/M³ Total Count % Total Count % Total Count % Total Count % Mycelial Fragments n/a <1 <33 33 n/a <1 <33 33 n/a n/a Pollen <1 <33 33 n/a <1 <33 33 n/a <1 <33 33 n/a <1 <33 33 n/a Total Fungal Spores ,267 D , , Alternaria Arthrinium Ascospores Fungal Spore Identification Fungal Spore Identification Fungal Spore Identification Fungal Spore Identification Aspergillus/Penicillium- Like ,000 D , , Basidiospores Bipolaris/Drechslera Botrytis Chaetomium Cladosporium < Curvularia Epicoccum Fusarium Memnoniella Nigrospora Oidium/Peronospora Pithomyces Rusts Smuts/Myxomycetes/Periconia Stachybotrys <1 Stemphylium Torula Ulocladium Unclassified Conidia Data Qualifier D211 Laboratory Manager: Project Manager: WC001 AH CLIENT REPORT FORM, P 1 of 2, Revision 04, 92204, LD WC001 Page 1 of 4

11 Beethe, Ronald L. CIH 400 Alvarado NE Albuquerque, NM Attn: Ronald Beethe Lab Number: AIHA EMLAP No Total Fungal Spore, Mycelia and Pollen Counts-Air Aerotech Method: WC001 Air-O-Cell Cassette Project Name: TP-0222 Date Received: 02/23/2007 Date Reported: 02/26/2007 Sample Number Sample Identification TPW-05 Pool To O/D Wall (E) TPW-06 Pool To O/D Wall # TPW-07 Mens Rm Entry Wall # TPW-08 Mens Lunch Wall # Date Analyzed 2/23/2007 2/23/2007 2/23/2007 2/23/2007 Volume(M³) Percent Of Trace Analyzed 100% of Trace at 600x Magnification 100% of Trace at 600x Magnification 100% of Trace at 600x Magnification 100% of Trace at 600x Magnification Debris Rating Analyte Count/M³ Count/M³ Count/M³ Count/M³ Total Count % Total Count % Total Count % Total Count % Mycelial Fragments <1 <33 33 n/a <1 <33 33 n/a 37 1, n/a <1 <33 33 n/a Pollen <1 <33 33 n/a <1 <33 33 n/a <1 <33 33 n/a <1 <33 33 n/a Total Fungal Spores <1 <33 33 n/a 6, ,367 D Fungal Spore Identification Fungal Spore Identification Fungal Spore Identification Fungal Spore Identification Alternaria <1 Arthrinium Ascospores Aspergillus/Penicillium- Like , ,333 D Basidiospores Bipolaris/Drechslera Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Memnoniella Nigrospora Oidium/Peronospora Pithomyces Rusts Smuts/Myxomycetes/Periconia Stachybotrys Stemphylium Torula Ulocladium Unclassified Conidia Data Qualifier D211 Laboratory Manager: Project Manager: WC001 AH CLIENT REPORT FORM, P 1 of 2, Revision 04, 92204, LD WC001 Page 2 of 4

12 Beethe, Ronald L. CIH 400 Alvarado NE Albuquerque, NM Attn: Ronald Beethe Lab Number: AIHA EMLAP No Total Fungal Spore, Mycelia and Pollen Counts-Air Aerotech Method: WC001 Air-O-Cell Cassette Project Name: TP-0222 Date Received: 02/23/2007 Date Reported: 02/26/2007 Sample Number Sample Identification TPW-09 Mens Rm Hall Wall # TPW-10 Mens Rm RR Wall # TPW-11 Mens Rm RR Wall # TWP-12 Mens Rm Showr Wall Date Analyzed 2/23/2007 2/23/2007 2/23/2007 2/23/2007 Volume(M³) Percent Of Trace Analyzed 100% of Trace at 600x Magnification 100% of Trace at 600x Magnification 100% of Trace at 600x Magnification 100% of Trace at 600x Magnification Debris Rating Analyte Count/M³ Count/M³ Count/M³ Count/M³ Total Count % Total Count % Total Count % Total Count % Mycelial Fragments <1 <33 33 n/a <1 <33 33 n/a n/a <1 <33 33 n/a Pollen <1 <33 33 n/a <1 <33 33 n/a <1 <33 33 n/a <1 <33 33 n/a Total Fungal Spores <1 <33 33 n/a Alternaria Arthrinium Ascospores Fungal Spore Identification Fungal Spore Identification Fungal Spore Identification Fungal Spore Identification Aspergillus/Penicillium- Like Basidiospores Bipolaris/Drechslera Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Memnoniella Nigrospora Oidium/Peronospora Pithomyces Rusts Smuts/Myxomycetes/Periconia Stachybotrys Stemphylium Torula Ulocladium Unclassified Conidia Data Qualifier Laboratory Manager: Project Manager: WC001 AH CLIENT REPORT FORM, P 1 of 2, Revision 04, 92204, LD WC001 Page 3 of 4

13 Beethe, Ronald L. CIH 400 Alvarado NE Albuquerque, NM Attn: Ronald Beethe Lab Number: AIHA EMLAP No Total Fungal Spore, Mycelia and Pollen Counts-Air Aerotech Method: WC001 Air-O-Cell Cassette Project Name: TP-0222 Date Received: 02/23/2007 Date Reported: 02/26/2007 Data Qualifiers Listed below are the data qualifiers included in the analytical report to explain any analytical or quality control issues. Data Qualifier Number D211 Description An estimation procedure was applied to determine counts for heavily loaded fungal structures. Laboratory Manager: Project Manager: WC001 AH CLIENT REPORT FORM, P 2 of 2, Revision 04, 92204, LD WC001 Page 4 of 4

14 Beethe, Ronald L. CIH 400 Alvarado NE Albuquerque, NM Attn: Ronald Beethe Lab Number: AIHA EMLAP No Total Fungal Spore, Mycelia and Pollen Counts-Dust Aerotech Method: CC001 Project Name: TP-0222 Date Received: 02/23/2007 Date Reported: 02/26/2007 Sample Number Sample Identification TPD-01 Women Family Rm Vent TPD-02 Mens Shower Vent TPD-03 Mens Rm Bedroom Vent TPD-04 Men's Family Rm Over Sink Date Analyzed 2/23/2007 2/23/2007 2/23/2007 2/23/2007 Dilution Factor Total Recoverable Dust(g) <0.06 Total Sample Analyzed <0.06 Total Sample Analyzed <0.06 Total Sample Analyzed <0.06 Total Sample Analyzed Count/Sample Count/Sample Count/Sample Count/Sample Analyte Total Count % Total Count % Total Count % Total Count % Mycelial Fragments n/a <2 <74 74 n/a <2 <74 74 n/a n/a Pollen <2 <74 74 n/a <2 <74 74 n/a <2 <74 74 n/a <2 <74 74 n/a Total Fungal Spores Alternaria Arthrinium Ascospores Fungal Spore Identification Fungal Spore Identification Fungal Spore Identification Fungal Spore Identification Aspergillus/Penicillium- Like Basidiospores Bipolaris/Drechslera Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Memnoniella Nigrospora Oidium/Peronospora Pithomyces Rusts Smuts/Myxomycetes/Periconia Stachybotrys Stemphylium Torula Ulocladium Unclassified Conidia Data Qualifier F67 F67 F67 F67 Laboratory Manager: Project Manager: CC001 AH CLIENT REPORT FORM, P 1 of 2, Revision 03, 92204, LD CC001 Page 1 of 2

15 Beethe, Ronald L. CIH 400 Alvarado NE Albuquerque, NM Attn: Ronald Beethe Lab Number: AIHA EMLAP No Total Fungal Spore, Mycelia and Pollen Counts-Dust Aerotech Method: CC001 Project Name: TP-0222 Date Received: 02/23/2007 Date Reported: 02/26/2007 Data Qualifiers Listed below are the data qualifiers included in the analytical report to explain any analytical or quality control issues. Data Qualifier Number F67 Description Total Sample weight was less than 0.06 grams. Results are reported per sample. Laboratory Manager: Project Manager: CC001 AH CLIENT REPORT FORM, P 2 of 2, Revision 03, 92204, LD CC001 Page 2 of 2

16 Beethe, Ronald L. CIH 400 Alvarado NE Albuquerque, NM Attn: Ronald Beethe Lab Number: AIHA EMLAP No Culturable Fungi at 25 C-Dust Aerotech Method: CC002 Project Name: TP-0222 Date Received: 02/23/2007 Date Reported: 03/02/2007 Sample Number Sample Identification TPD-01 Women Family Rm Vent TPD-02 Mens Shower Vent TPD-03 Mens Rm Bedroom Vent TPD-04 Men's Family Rm Over Sink Date Prepped 2/23/2007 2/23/2007 2/23/2007 2/23/2007 Date Analyzed 3/2/2007 3/2/2007 3/2/2007 3/2/2007 Culture Media Potato Dextrose (PDA) Potato Dextrose (PDA) Potato Dextrose (PDA) Potato Dextrose (PDA) Dilution Factor Total Recoverable Dust(g) <0.06 Total Sample Analyzed <0.06 Total Sample Analyzed <0.06 Total Sample Analyzed <0.06 Total Sample Analyzed CFU/Sample CFU/Sample CFU/Sample CFU/Sample Analyte % % % % Total Acremonium Alternaria Aspergillus niger Aspergillus species Var Aspergillus species Var. 2 Aureobasidium Bipolaris Chaetomium Cladosporium Curvularia Epicoccum Fusarium Geotrichum Mucor Nigrospora Oidiodendron Paecilomyces Penicillium species Var Penicillium species Var. 2 Pithomyces Rhizopus Sporothrix Sporotrichum Stachybotrys Sterile Hyphae Syncephalastrum Trichoderma Yeast Data Qualifier F67 F67 F67 F67 Laboratory Manager: Project Manager: CC002 AH CLIENT REPORT FORM, P 1 of 2, Revision 03, 92204, LD CC002 Page 1 of 2

17 Beethe, Ronald L. CIH 400 Alvarado NE Albuquerque, NM Attn: Ronald Beethe Lab Number: AIHA EMLAP No Culturable Fungi at 25 C-Dust Aerotech Method: CC002 Project Name: TP-0222 Date Received: 02/23/2007 Date Reported: 03/02/2007 Data Qualifiers Listed below are the data qualifiers included in the analytical report to explain any analytical or quality control issues. Data Qualifier Number F67 Description Total Sample weight was less than 0.06 grams. Results are reported per sample. Laboratory Manager: Project Manager: CC002 AH CLIENT REPORT FORM, P 2 of 2, Revision 03, 92204, LD CC002 Page 2 of 2

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