4b. Innate (nonspecific) Immunity
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1 4b. Innate (nonspecific) Immunity
2 Chapter 16: Innate (nonspecific) Immunity! Some terms:! Susceptibility: Lack of immunity to a disease.! Immunity: Ability to ward off disease.! Innate immunity: Defenses against any pathogen.! Does not involve specific recognition of a microbe! No memory response! Adap7ve immunity! Specific response to a specific microbe once a microbe has breached the innate immunity defenses!! Slower to respond but develops memory!
3 An overview of the body s Figure 16.1! defenses! 4b 4c
4 The Concept of Innate Immunity! Host Toll-like receptors (TLRs) attach to! Pathogenassociated molecular patterns (PAMPs)! TLRs induce cytokines that regulate the intensity and duration of immune responses! On Your Cells! On Pathogen!
5 A. Physical barriers (first line of defense)! skin, mucous membranes = prevent entry to most pathogens! Epidermis consists of tightly packed cells with! Keratin, a protective protein!
6 B. Lysozyme! enzyme in tears, sweat, saliva, etc that dissolves bacterial cell walls (digests peptidoglycan)!
7 C. Respiratory cilia! carry pathogens out of lungs!
8 Respiratory cilia!
9
10 D. Stomach acid! HCl at ph 1-2! kills many, but not all pathogens!
11 Physical factors! Mucous membranes! Ciliary escalator: Microbes trapped in mucus are transported away from the lungs.! Lacrimal apparatus: Washes eye.! Saliva: Washes microbes off.! Urine: Flows out.! Vaginal secretions: Flow out.!
12 Chemical Factors! Fungistatic fatty acid in sebum! Low ph (3 5) of skin! Lysozyme in perspiration, tears, saliva, and urine! Low ph ( ) of gastric juice! Low ph (3 5) of vaginal secretions!
13 Normal Microbiota and Innate Immunity! Microbial antagonism/competitive exclusion: Normal microbiota compete with pathogens or alter the environment! Commensal microbiota: One organism (microbe) benefits and the other (host) is unharmed! May be opportunistic pathogens!
14
15 Mammalian Blood Composition Plasma (55%) Water Ions Plasma Proteins Nutrients Waste Gases Hormones Cellular Elements (45%) Erythrocytes Leukocytes Thrombocytes
16 Formed Elements in Blood!
17
18 Differential White Cell Count! Percentage of each type of white cell in a sample of 100 white blood cells! Neutrophils 60 70% Basophils 0.5 1% Eosinophils 2 4% Monocytes 3 8% Lymphocytes 20 25%
19 White Blood Cells! Neutrophils: Phagocytic (most abundant)! Basophils: Produce histamine! Eosinophils: Toxic to parasites and some phagocytosis (worm infections)! Dendritic cells: Initiate adaptive immune response! Monocytes: Phagocytic as mature macrophages! Fixed macrophages in lungs, liver, and bronchi! Wandering macrophages roam tissues.! Lymphocytes: Involved in specific immunity.!
20 Figure 16.7!
21 Microbial Evasion of Phagocytosis! Inhibit adherence: M protein, capsules Kill phagocytes: Leukocidins Lyse phagocytes: Membrane attack complex Escape phagosome Prevent phagosome-lysosome fusion Survive in phagolysosome Streptococcus pyogenes, S. pneumoniae Staphylococcus aureus Listeria monocytogenes Shigella, Rickettsia HIV, Mycobacterium tuberculosis Coxiella burnettii
22 Inflammation!
23 Inflammation!
24 Inflammation! Redness! Pain!! Heat! Swelling (edema)! Acute-phase proteins activated (complement, cytokine, and kinins)! Vasodilation (histamine, kinins, prostaglandins, and leukotrienes)! Margination and emigration of WBCs! Tissue repair!
25 chemotaxis: phagocytes attracted to pathogen!
26 Tissue Repair!
27
28 Fever! Abnormally high body temperature! Hypothalamus normally set at 37 C! Gram-negative endotoxin cause phagocytes to release interleukin 1 (IL 1)! Hypothalamus releases prostaglandins that reset the hypothalamus to a high temperature! Body increases rate of metabolism and shivering which raise temperature! Vasodilation and sweating: Body temperature falls (crisis)!
29 Fever! A moderate fever is beneficial to host defenses! speeds up body defenses; slows down growth of pathogens! Fever producing pyrogens are produced by activated macrophages as well as bacteria, viruses, and other microbes! Stimulate hypothalamus to raise body temp.! Thus cell metabolism increases and blood vessels constrict keeping heat within (cold skin/chills with fever)! But, above F, convulsions and death may result! Infants >100 0 F and older children >102 0 F need medical attention!!
30 Antimicrobial substances: The Complement System! Complement (complement system): a series of over 30 blood proteins (produced by the liver) that circulate in the bloodstream and sequentially bond together to causes lysis of pathogens, inflammation and help phagocytosis! complements the cells of the immune system in destroying microbes! can be a specific or nonspecific defense!
31 Compliment Sysmtem!
32 Interferons (IFNs)! messenger proteins produced by virusinfected cells! tell surrounding cells to produce anti-viral protein! also produced by genetic engineered microorganisms for injection as antiviral drugs!
33 Summary of Second Line of Defense!
34 Stress theory of disease! Hans Selye! A. Stressor: any force that elicits the stress response! invasion by pathogen! trauma! surgery! emotional conflict! performance demands! difficult relationships! life changes (positive or negative)!
35 B. Stress! the body s response to any stressor! includes many measurable physiological changes intended to increase resistance to stressors! also called the General Adaptation Syndrome! 1. : Eustress beneficial stress; appropriate in degree and duration; produces optimum physical and mental function and resistance to pathogens! 2. : Distress harmful stress; excessive in degree and/or duration; impairs physical and mental function; reduces resistance to pathogens! Stress management!
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