ISHAM Symposium: S33: Ocular aspects of Fungal Infections Friday, 8 May 2015, ; MR101/102 Level 1

Size: px
Start display at page:

Download "ISHAM Symposium: S33: Ocular aspects of Fungal Infections Friday, 8 May 2015, ; MR101/102 Level 1"

Transcription

1 ISHAM Symposium: S33: Ocular aspects of Fungal Infections Friday, 8 May 2015, ; MR101/102 Level 1 Chairs: Ariya Chindamporn, TH and Phillip A Thomas, IN 14:15-14:35 AGENDA Clinical overview of ocular fungal infections #935 Peter McCluskey, AUS 14:35-14:50 Neglected fungal infections of eye #722 Ariya Chindamporn, TH 14:50-15:10 15:10-15:25 Fungal virulence factors in ocular infections #936 The challenges in Mycotic keratitis in horses and dogs #938 Philip A Thomas, IN Andrew Turner, AUS 15:25-15:45 Host factors in mycotic keratitis Lalitha Prajna, IN

2 19 th Melbourne Ocular aspects of Fungal Infections Neglected fungal infections of eye Ariya Chindamporn Department of Microbiology Faculty of Medicine, Chulalongkorn University May 8, 2015

3 Outline Laboratory investigation Risk factors Epidemiology Cases

4 Flow chart : laboratory investigation Cornea scrapings, biopsy Direct from specimens Fungal Isolation KOH preparation: 75-90% Macroscopic Exam. Calcofluor: 80-90% Microscopic Exam. GMS: 89-98% Zoospore production PCR & Sequencing PCR & Sequencing %sensitivity (Thomas PA., 2003 Eye:17 (852-62)

5 Corneal scraping, staining, inoculation

6 Specimen collection for PCR Cornea, Vitreous, Aqueous For PCR FFPE

7 Risk factors HISTORY is important. o Trauma : Dust, Leaf, Stone, Water, Contact len o Swimming o Occupation : working outdoor/indoor o Host factors: diabetes mellitus o Fungal virulence factors o > 5-10 days

8 Frequence isolates of Fungal MOLD Hyaline hyphae : Fusarium spp.: F. solani, F. oxysporum Aspergillus spp. : A. fumigatus, A. flavus Scedosporium spp. : S. apiospermum Penicillium spp. : P. spinulosum, P. citrinum Acremoniium spp. ( A. potronii, A. kiliense) Dematiaceous fungi Curvularia spp. (C. lunata, C. geniculate, C. senegalensis ) Bipolaris spp. ( B. spicifera, B. hawaiiensis) Exserohilum spp. (E. rostratum, E. longirostrata) Coelomycetes (Lasiodiplodia, Colletotrichum) Alternaria Yeast Candida spp. (C. albicans) Rhodotorula Others Pythium insidiosum Lagenidium albertoi Modified from Thomas PA., 2003 Eye :17:852-62

9 Epidemiology 1352 proven cases, a tertiary care hosp. South India -10 yr. ( ) (Gopinathan et al., 2002 Cornea:21:555-9) > 99% caused by mold; Male > Female: 2.5:1; Age: Risk: systemic factors : DM 654 eyes in Shandong Eye Institute, North China, Jan Dec 2004 (Zhong XL., et al 2006 Opthalmol 113(11):1943-8) 62% of total cases : fungal keratitis Fusarium spp. : F. solani 73%, 437 eyes) Aspergillus spp. : A. flavus 12%, 72 eyes Prevalence : the season, more : Oct to Dec than Jul to Sep. KOH preparation 88% positive, Culture 91% Histopath. staining: most effective, resulting 98% but need to reveal genus and species. 997 cases in eastern India, Jul 2006 and Dec % as fungal keratitis. The geographical place and climate affected the prevalence (2012) : 36.7% in Southern, 36.3%, 25.6%, 7.3% in Western, North-eastern, and Northern India, in order. Katara SR., et al., (6); Acta Medica Iranica

10 Mimiking fungal infection

11 Conventional approach KOH-preparation: rare septate hyphae Histopathology: GMS Pythium insidiosum Kingdom Straminipila/ Stramenopila

12 Natural resource Pythiosis in Thailand : Clinical case HIGHEST INCIDENCE in Human Supanpandu et al., Med Mycol 2008 C : Central NE: Northeastern S : Southern N : Northern E : Eastern 12 Krajejun et al., CID 2006

13 Specimens KOH Culture PCR Cornea Scraping/ biopsy 1 st d 2 nd d 2wk 1 st d 2 nd d 2 wk 1 st d 2 nd d 2 wk Aqueous Vitreous Vitreous and Iris Lens Lens IFN ITS Cornea Rt.Lens KOH: Positive

14 Rt.Cornea KOH: Positive Culture: Positive PCR: Positive

15 IMPORTED CASE

16

17

18

19

20

21 Lagenidium sp. Ocular Infection Mimicking Ocular Pythiosis U Riengprayoon et al., JCM 2013:51(8): yr. housewife admitted in a tertiary care hospital: lid swelling, pain, redness, & itching for 3 weeks, visit physician,.. visual acuity: finger counting at 1 foot slit lamp ex.: ciliary injection & a yellowish, midstromal, reticular pattern corneal infiltration measuring 5x5.6 mm. with feathery edge corneal epitherial defect 4x3 mm in dia. over the infiltrated area posterior eye segment by ultrasound: no sign of endophthalmitis unsuccessfully treated with systemic and topical drugs

22 Lagenidium sp.: Mimicking Ocular Infection Fungal elements in corneal stroma by confocal microscopy Broad, rare septate hyphae by KOH preparation Broad hyphae by GMS U Riengprayoon et al., JCM 2013:51(8):2778

23 Treatment - First : systemic & topical antibiotics: worse - Presumed to be fungal infection: - topical : cefazolin, amikacin, natamycin, voriconazole, Referred to tertiary hospital - Result from ocular confocal micro. & frequency of pythiosis - oral terbinafine, itraconazole & topical natamycin with Pythium Insidiosum Antigen (PIA) vaccine - worse - Twice of Penetrating keratoplasty (PK) - 10 mo. later : no recurrent of infection

24 Susceptibility result *The value of Minimal Inhibitory Concentration (MIC) Agents Lagenidium spp. Clinical P. insidiosum Enviromental P. insidiosum Amphotericin B Terbinafine Voriconazole Itraconazole Fluconazole Anidulafungin Caspofungin TB/VO Indifference Indifference Indifference TV/IT Indifference Indifference Indifference

25 Parsimony Analysis ITS 1000 Bootstrap Parsimony analysis, ITS 1000 Bootstrap Lagenidium albertoi Vol. 21, No. 2, February 2015

26 Sharma et al., 2014 Calcofluor white staining 35C, 3 d. PDA LCB wet mount Sporangia with zoospore

27 Take home message Rapid and accurate diagnosis, leading the right treatment is required: Communication with physicians Direct exam. & Culture still gold standard Molecular identification Very rapid growth, no sporulation No response to antifungal agents: Think about parafungal infection

28

Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok,

Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, JCM Accepts, published online ahead of print on 5 June 2013 J. Clin. Microbiol. doi:10.1128/jcm.00783-13 Copyright 2013, American Society for Microbiology. All Rights Reserved. 1 Lagenidium sp. ocular

More information

Prevalence of Oculomycosis in a Tertiary Care Centre

Prevalence of Oculomycosis in a Tertiary Care Centre AJMS Al Ameen J Med Sci (2 011 )4 (4 ):3 3 4-3 3 8 (A US National Library of Medicine enlisted journal) I S S N 0 9 7 4-1 1 4 3 C O D E N : A A J M B G ORIGI NAL ARTICLE Prevalence of Oculomycosis in a

More information

Mycotic Keratitis in Patients Attending a Tertiary Care Hospital

Mycotic Keratitis in Patients Attending a Tertiary Care Hospital International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 10 (20) pp. 1665-1670 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.20.610.201

More information

Introduction. Study of fungi called mycology.

Introduction. Study of fungi called mycology. Fungi Introduction Study of fungi called mycology. Some fungi are beneficial: ex a) Important in production of some foods, ex: cheeses, bread. b) Important in production of some antibiotics, ex: penicillin

More information

The Epidemiological Features and Laboratory Results of Fungal Keratitis

The Epidemiological Features and Laboratory Results of Fungal Keratitis Cornea 21(6): 555 559, 2002. 2002 Lippincott Williams & Wilkins, Inc., Philadelphia The Epidemiological Features and Laboratory Results of Fungal Keratitis A 10-Year Review at a Referral Eye Care Center

More information

Lamellar Keratoplasty for the Treatment of Fungal Keratitis

Lamellar Keratoplasty for the Treatment of Fungal Keratitis Cornea 21(1): 33 37, 2002. 2002 Lippincott Williams & Wilkins, Inc., Philadelphia Lamellar Keratoplasty for the Treatment of Fungal Keratitis Lixin Xie, M.D., Weiyun Shi, M.D., Zhaosheng Liu, M.D., and

More information

Archives of Infect Diseases & Therapy, 2017

Archives of Infect Diseases & Therapy, 2017 Research Article Archives of Infectious Diseases & Therapy Antifungal sensitivity profile of Fusarium spp. resulting keratitits LSM Sigera 1, PI Jayasekera 1, ULF Shabry 1 and MAI Malkanthi 1 1 Dept. of

More information

ISPUB.COM. Management of Mycotic Keratitis. V Sharma, M Purohit, S Vaidya INTRODUCTION

ISPUB.COM. Management of Mycotic Keratitis. V Sharma, M Purohit, S Vaidya INTRODUCTION ISPUB.COM The Internet Journal of Ophthalmology and Visual Science Volume 6 Number 2 V Sharma, M Purohit, S Vaidya Citation V Sharma, M Purohit, S Vaidya.. The Internet Journal of Ophthalmology and Visual

More information

Use of Antifungals in the Year 2008

Use of Antifungals in the Year 2008 Use of Antifungals in the Year 2008 Jose G. Montoya, MD Associate Professor of Medicine Associate Chief for Clinical Affairs Division of Infectious Diseases Stanford University School of Medicine Diagnosis

More information

Fungal Keratitis CURRENT CONCEPTS. Aetiology. Epidemiology. Fungi causing human keratitis. Predisposing factors. Local

Fungal Keratitis CURRENT CONCEPTS. Aetiology. Epidemiology. Fungi causing human keratitis. Predisposing factors. Local June 2008 N. Bindu - Fungal Keratitis 169 CURRENT CONCEPTS Fungal Keratitis Dr. N. Bindu MS Fungal infections of the cornea constitute an important eye problem in outdoor workers in tropical & subtropical

More information

The Role of Ultrasonographic Biomicroscopy in the management of a patient with presumed Dematiaceous Mycotic Keratitis

The Role of Ultrasonographic Biomicroscopy in the management of a patient with presumed Dematiaceous Mycotic Keratitis ISPUB.COM The Internet Journal of Ophthalmology and Visual Science Volume 6 Number 2 The Role of Ultrasonographic Biomicroscopy in the management of a patient with presumed Dematiaceous Mycotic Keratitis

More information

Fungal Infection Pre-Infusion Data

Fungal Infection Pre-Infusion Data Fungal Infection Pre-Infusion Data Registry Use Only Sequence Number: Date Received: CIBMTR Center Number: Event date: / / CIBMTR Form 2046 revision 5 (page 1 of 5). Last Updated May, 2018. Infection Episode

More information

Spectrum of Fungal Keratitis in North China

Spectrum of Fungal Keratitis in North China Spectrum of Fungal Keratitis in North China Lixin Xie, MD, 1 Wenxian Zhong, MD, 1,2 Weiyun Shi, MD, 1 Shiying Sun, MD 1 Purpose: To report the epidemiological features, laboratory findings, and treatment

More information

GAFFI Fact Sheet. Keratitis refers to inflammation (usually an infection) of the normally transparent DARKER AREAS AND

GAFFI Fact Sheet. Keratitis refers to inflammation (usually an infection) of the normally transparent DARKER AREAS AND Fungal Keratitis GAFFI Fact Sheet GLOBAL ACTION FUND FOR FUNGAL INFECTIONS Keratitis refers to inflammation (usually an infection) of the normally transparent DARKER AREAS AND cornea of the eye, which

More information

Identification of Fungal Species in Proved Cases of Fungal Corneal Ulcer

Identification of Fungal Species in Proved Cases of Fungal Corneal Ulcer www.jmscr.igmpublication.org Impact Factor 3.79 ISSN (e)-2347-176x Identification of Fungal Species in Proved Cases of Fungal Corneal Ulcer Authors Madhusudhan C.N 1, Tanushree V 2, H.T.Venkategowda 3,

More information

Treatment of fungal keratitis by penetrating keratoplasty

Treatment of fungal keratitis by penetrating keratoplasty 1070 Shandong Eye Institute and Hospital, Qingdao 266071, PR China L Xie X Dong W Shi Correspondence to: Lixin Xie, MD, Shandong Eye Institute and Hospital, 5 Yanerdao Road, Qingdao 266071, PR China lixinxie@public.qd.sd.cn

More information

FUNGAL CORNEAL ULCER. Arundhati Dvivedi final year p.g Dept.of Ophthalmology 2018/7/31

FUNGAL CORNEAL ULCER. Arundhati Dvivedi final year p.g Dept.of Ophthalmology 2018/7/31 FUNGAL CORNEAL ULCER Arundhati Dvivedi final year p.g Dept.of Ophthalmology Introduction: Fungal Keratitis is one of the most difficult forms of microbial keratitis to diagnose & to treat successfully

More information

Mycotic keratitis is an important ophthalmologic problem

Mycotic keratitis is an important ophthalmologic problem CLINICAL SCIENCES Spectrum of Fungal Keratitis in North India Anuradha Chowdhary, MD,* and Kirti Singh, MD, DNB, FRCS Purpose: To report the epidemiologic features and laboratory results of 191 consecutive

More information

Treatment of rare and emerging fungal infections. EFISG Educational Workshop 15 th ECCMID April 2, 2005, Copenhagen

Treatment of rare and emerging fungal infections. EFISG Educational Workshop 15 th ECCMID April 2, 2005, Copenhagen Treatment of rare and emerging fungal infections EFISG Educational Workshop 15 th ECCMID April 2, 2005, Copenhagen Helen Sambatakou Lecturer in Medicine and Infectious Diseases, University of Athens, Greece

More information

Antifungal Activity of Voriconazole on Local Isolates: an In-vitro Study

Antifungal Activity of Voriconazole on Local Isolates: an In-vitro Study Original Article Philippine Journal of OPHTHALMOLOGY Antifungal Activity of Voriconazole on Local Isolates: an In-vitro Study Karina Q. De Sagun-Bella, MD, 1 Archimedes Lee D. Agahan, MD, 1 Leo DP. Cubillan,

More information

INCIDENCE OF CURVULARIA ORGANISM IN MYCOTIC CORNEAL ULCER K. Anjaneyulu 1, Balla Vidya Sagar 2

INCIDENCE OF CURVULARIA ORGANISM IN MYCOTIC CORNEAL ULCER K. Anjaneyulu 1, Balla Vidya Sagar 2 INCIDENCE OF CURVULARIA ORGANISM IN MYCOTIC CORNEAL ULCER K. Anjaneyulu 1, Balla Vidya Sagar 2 HOW TO CITE THIS ARTICLE: K. Anjaneyulu, Balla Vidya Sagar. Incidence of Curvularia Organism in Mycotic Corneal

More information

Pathogenesis of ocular fungal Infections. Dr Lalitha Prajna. MD. Department of Ocular Microbiology, Aravind Eye Hospital, Madurai. Tamil Nadu.

Pathogenesis of ocular fungal Infections. Dr Lalitha Prajna. MD. Department of Ocular Microbiology, Aravind Eye Hospital, Madurai. Tamil Nadu. Pathogenesis of ocular fungal Infections Dr Lalitha Prajna. MD. Department of Ocular Microbiology, Aravind Eye Hospital, Madurai. Tamil Nadu. Introduction Fungal infections of the eye are rare ( exception:

More information

Ariya Chindamporn, Ph.D. Dept. of Microbiology, Fac. of Medicine, Chulalongkorn University, Bangkok, Thailand. Taipei, Taiwan Nov. 16, 2018; 11:45 am.

Ariya Chindamporn, Ph.D. Dept. of Microbiology, Fac. of Medicine, Chulalongkorn University, Bangkok, Thailand. Taipei, Taiwan Nov. 16, 2018; 11:45 am. Mimic fungal infections in Asia Ariya Chindamporn, Ph.D. Dept. of Microbiology, Fac. of Medicine, Chulalongkorn University, Bangkok, Thailand Outline: Introduction Taipei, Taiwan Nov. 16, 2018; 11:45 am.

More information

An Update in the Management of Candidiasis

An Update in the Management of Candidiasis An Update in the Management of Candidiasis Daniel B. Chastain, Pharm.D., AAHIVP Infectious Diseases Pharmacy Specialist Phoebe Putney Memorial Hospital Adjunct Clinical Assistant Professor UGA College

More information

Clinical Decision making in Infectious Keratitis

Clinical Decision making in Infectious Keratitis Clinical Decision making in Infectious Stephen D. McLeod, MD Theresa M. and Wayne M. Caygill, MD Distinguished Professor and Chair Department of Ophthalmology Francis I. Proctor Foundation University of

More information

Fungal Infection Post-Infusion Data

Fungal Infection Post-Infusion Data Fungal Infection Post-Infusion Data Registry Use Only Sequence Number: Date Received: CIBMTR Center Number: Event date: / / Visit: 100 day 6 months 1 year 2 years >2 years. Specify: CIBMTR Form 2146 revision

More information

Spectrum of Mycotic corneal ulcers in Mid Western peripheral region of Terrain belt of Nepal and Indo-Nepal Border

Spectrum of Mycotic corneal ulcers in Mid Western peripheral region of Terrain belt of Nepal and Indo-Nepal Border Nepal Journal of Medical Sciences Original Article Spectrum of Mycotic corneal ulcers in Mid Western peripheral region of Terrain belt of Nepal and Indo-Nepal Border Bastola P, 1* Mishra A, 2 Chaudhary

More information

Spectrum of Fungal Keratitis at Wills Eye Hospital, Philadelphia, Pennsylvania

Spectrum of Fungal Keratitis at Wills Eye Hospital, Philadelphia, Pennsylvania Cornea 19(3): 307 312, 2000. 2000 Lippincott Williams & Wilkins, Inc., Philadelphia Spectrum of Fungal Keratitis at Wills Eye Hospital, Philadelphia, Pennsylvania Marco Antonio G. Tanure, M.D., Elisabeth

More information

International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR) 1 M.D. (Microbiology), Professor & HOD of Microbiology, Govt. Thoothukudi Medical College 2 M.S., D.O., FICO., Associate Prof., Department of Ophthalmology, Tirunelveli Medical College 3 M.S., Assistant

More information

Original Article. Fungal keratitis in Melbourne INTRODUCTION ABSTRACT METHODS

Original Article. Fungal keratitis in Melbourne INTRODUCTION ABSTRACT METHODS Original Article Clinical and Experimental Ophthalmology 2007; 35: 124 130 doi:10.1111/j.1442-9071.2006.01405.x Fungal keratitis in Melbourne Prashant Bhartiya FRCS, 1,2 Mark Daniell FRANZCO, 1,2 Marios

More information

A Clinical Microbiological Study of Corneal Ulcer Patients at Western Gujarat, India

A Clinical Microbiological Study of Corneal Ulcer Patients at Western Gujarat, India A Clinical Microbiological Study of Corneal Ulcer Patients at Western Gujarat, India Rajesh Somabhai Katara 1, Nilesh Dhanjibhai Patel 2, and Mala Sinha 3 1 Department of Microbiology, B. J. Medical College,

More information

KERATITIS. Edited by Muthiah Srinivasan

KERATITIS. Edited by Muthiah Srinivasan KERATITIS Edited by Muthiah Srinivasan KERATITIS Edited by Muthiah Srinivasan Keratitis Edited by Muthiah Srinivasan Published by InTech Janeza Trdine 9, 51000 Rijeka, Croatia Copyright 2012 InTech All

More information

About the Editor Gerri S. Hall, Ph.D.

About the Editor Gerri S. Hall, Ph.D. About the Editor Gerri S. Hall, Ph.D. Dr. Hall s professional career has been focused on clinical microbiology: direct clinical activities of various areas such as bacteriology, mycobacteria, STD testing,

More information

Antifungal Resistance in Asia: Mechanisms, Epidemiology, and Consequences

Antifungal Resistance in Asia: Mechanisms, Epidemiology, and Consequences 5th MMTN Conference 5-6 November 2016 Bangkok, Thailand 10:20-10:45, 6 Nov, 2016 Antifungal Resistance in Asia: Mechanisms, Epidemiology, and Consequences Yee-Chun Chen, M.D., PhD. Department of Medicine,

More information

Subcutaneous Fungi 10/13/2009. General Characteristics. Pathogenesis. Epidemiology. Laboratory Diagnosis. Specimens. Growth rate: 1-4 weeks

Subcutaneous Fungi 10/13/2009. General Characteristics. Pathogenesis. Epidemiology. Laboratory Diagnosis. Specimens. Growth rate: 1-4 weeks General Characteristics Growth rate: 1-4 weeks Subcutaneous Fungi Clinical Laboratory Science Program Carol Larson MSEd, MT(ASCP) Dematiaceous septate hyphae Hyaline septate hyphae Branching GPR Epidemiology

More information

CASE PRESENTATION BY Dr. Prashanti OPHTHALMOLOGY Ist YR

CASE PRESENTATION BY Dr. Prashanti OPHTHALMOLOGY Ist YR CASE PRESENTATION BY Dr. Prashanti OPHTHALMOLOGY Ist YR PERSONAL DETAILS NAME : xxx AGE :57 SEX : Male IP/OP NO- 20170828623 OCCUPTION : Farmer CHIEF COMPLAINTS Redness Pain Watering Blurring of vision

More information

CLINICAL SCIENCES z. Aqueous and Vitreous Concentrations Following Topical Administration of 1% Voriconazole in Humans

CLINICAL SCIENCES z. Aqueous and Vitreous Concentrations Following Topical Administration of 1% Voriconazole in Humans CLINICAL SCIENCES z Aqueous and Vitreous Concentrations Following Topical Administration of 1% Voriconazole in Humans G. Atma Vemulakonda, MD; Seenu M. Hariprasad, MD; William F. Mieler, MD; Randall A.

More information

Otomycosis in Bikaner: A Clinico-Mycological Study

Otomycosis in Bikaner: A Clinico-Mycological Study International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 9 (2017) pp. 2943-2947 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.609.361

More information

Diagnosis and Treatment of Pythium Insidiosum Corneal Ulcer in a Chinese Child: A Case Report and Literature Review

Diagnosis and Treatment of Pythium Insidiosum Corneal Ulcer in a Chinese Child: A Case Report and Literature Review ISSN 1941-5923 DOI: 10.12659/AJCR.901158 Received: 2016.08.21 Accepted: 2016.09.19 Published: 2016.12.27 Diagnosis and Treatment of Pythium Insidiosum Corneal Ulcer in a Chinese Child: A Case Report and

More information

Update zu EUCAST 2012 Cornelia Lass-Flörl

Update zu EUCAST 2012 Cornelia Lass-Flörl Update zu EUCAST 2012 Cornelia Lass-Flörl Frühjahrstagung 2012 Paul-Ehrlich-Gesellschaft Sektion Antimykotische Chemotherapie Bonn, 4./5. Mai 2012 Agenda 1. Breakpoints 2. Rationale documents and technical

More information

Topical 5% Natamycin With Oral Ketoconazole in Filamentous Fungal Keratitis: A Randomized Controlled Trial

Topical 5% Natamycin With Oral Ketoconazole in Filamentous Fungal Keratitis: A Randomized Controlled Trial ORIGINAL CLINICAL STUDY With Oral in Filamentous Fungal Keratitis: A Randomized Controlled Trial Revathi Rajaraman, MS,* Parameshwar Bhat, MS,* Vikram Vaidee, DNB,* Sowmyalatha Maskibail, MS,* Anita Raghavan,

More information

Correlation of culture with histopathology in fungal burn wound colonization and infection

Correlation of culture with histopathology in fungal burn wound colonization and infection burns 33 (2007) 341 346 available at www.sciencedirect.com journal homepage: www.elsevier.com/locate/burns Correlation of culture with histopathology in fungal burn wound colonization and infection Christina

More information

LABORATORY SCIENCES. Antimicrobial Susceptibility of Fusarium, Aspergillus, and Other Filamentous Fungi Isolated From Keratitis

LABORATORY SCIENCES. Antimicrobial Susceptibility of Fusarium, Aspergillus, and Other Filamentous Fungi Isolated From Keratitis LABORATORY SCIENCES Antimicrobial Susceptibility of Fusarium, Aspergillus, and Other Filamentous Fungi Isolated From Keratitis Prajna Lalitha, MD; Brett L. Shapiro, BA; Muthiah Srinivasan, MD; Namperumalsamy

More information

C.V. Parvin Dehghan. Director & head of Mycology and Parasitology Dep. ( ) Faculty of Medicine, Isfahan University of Medical Sciences

C.V. Parvin Dehghan. Director & head of Mycology and Parasitology Dep. ( ) Faculty of Medicine, Isfahan University of Medical Sciences C.V Parvin Dehghan Faculty of Medicine, Isfahan University of medical Sciences, Iran. dehghan@med.mui.ac.ir Professional Experience Director & head of Mycology and Parasitology Dep. (2014-2016) Faculty

More information

Clinical and Microbiological Profile of Various Microorganisms Causing Keratitis in a Tertiary Care Hospital, Jaipur, India

Clinical and Microbiological Profile of Various Microorganisms Causing Keratitis in a Tertiary Care Hospital, Jaipur, India International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 2 (2017) pp. 1333-1342 Journal homepage: http://www.ijcmas.com Original Research Article http://dx.doi.org/10.20546/ijcmas.2017.602.151

More information

Candida auris: an Emerging Hospital Infection

Candida auris: an Emerging Hospital Infection National Center for Emerging and Zoonotic Infectious Diseases Candida auris: an Emerging Hospital Infection Paige Armstrong MD MHS Epidemic Intelligence Service Officer Mycotic Diseases Branch Association

More information

Histopathology Description:

Histopathology Description: 2013-2-1 CANINE HEART Ahmed M. Abubakar BOVINE PATHOLOGY CONTRIBUTING INSTITUTION : The Royal Veterinary college, Dept. of Pathology and Biology Signalment: 11-month-old male Border Collie dog (Canis familiaris)

More information

DALK IN DANGEROUS INFECTIONS

DALK IN DANGEROUS INFECTIONS 32 INTERNATIONAL CONGRESS of the HELLENIC SOCIETY OF INTRAOCULAR IMPLANT AND REFRACTIVE SURGERY CORNEA ROUND TABLE: STROMAL REPAIR DALK IN DANGEROUS INFECTIONS, MD Clinica Degli Occhi Sarnicola, Grosseto

More information

Use of Antifungal Drugs in the Year 2006"

Use of Antifungal Drugs in the Year 2006 Use of Antifungal Drugs in the Year 2006" Jose G. Montoya, MD Associate Professor of Medicine Associate Chief for Clinical Affairs Division of Infectious Diseases Stanford University School of Medicine

More information

EPIDEMIOLOGICAL AND MICROBIOLOGICAL PROFILE OF PATIENT S HAVING MICROBIAL KERATITIS

EPIDEMIOLOGICAL AND MICROBIOLOGICAL PROFILE OF PATIENT S HAVING MICROBIAL KERATITIS Original Article EPIDEMIOLOGICAL AND MICROBIOLOGICAL PROFILE OF PATIENT S HAVING MICROBIAL KERATITIS Saurabh Patel 1, Akshay M Chaudhari 2, Trupti M Solu 3, Vaibhav Gharat 4 Financial Support: None declared

More information

Fungal Infection of Sutureless Self-sealing Incision for Cataract Surgery

Fungal Infection of Sutureless Self-sealing Incision for Cataract Surgery Fungal Infection of Sutureless Self-sealing Incision for Cataract Surgery Prashant Garg, MS, 1 S. Mahesh, MD, 1 Aashish K. Bansal, MS, 1 Usha Gopinathan, PhD, 2 Gullapalli N. Rao, MD 1 Purpose: To report

More information

number Done by Corrected by Doctor د.حامد الزعبي

number Done by Corrected by Doctor د.حامد الزعبي number Fungi#1 Done by نرجس الس ماك Corrected by مهدي الشعراوي Doctor د.حامد الزعبي Introduction to Mycology -Terms: -Medical Mycology: The study of mycosis and their etiological agents -Mycosis: Disease

More information

AUSTRALIAN ANTIFUNGAL SUSCEPTIBILITY DATA : PART 2 THE MOULDS ASPERGILLUS, SCEDOSPORIUM AND FUSARIUM.

AUSTRALIAN ANTIFUNGAL SUSCEPTIBILITY DATA : PART 2 THE MOULDS ASPERGILLUS, SCEDOSPORIUM AND FUSARIUM. AUSTRALIAN ANTIFUNGAL SUSCEPTIBILITY DATA 00-0: PART THE MOULDS ASPERGILLUS, SCEDOSPORIUM AND FUSARIUM. AUSTRALIAN Sarah Kidd, Rose Handke and ANTIFUNGAL David Ellis SUSCEPTIBILITY DATA 00-00 David SA

More information

Trichophyton Microsporum Epidermophyton. dermatomycosis. Dematiaceous(pigmented fungi ) Dimorphic fungi Yeast and yeast like saprophyte

Trichophyton Microsporum Epidermophyton. dermatomycosis. Dematiaceous(pigmented fungi ) Dimorphic fungi Yeast and yeast like saprophyte Cutaneous candidiasis dermatophytosis Trichophyton Microsporum Epidermophyton dermatomycosis Dematiaceous(pigmented fungi ) Dimorphic fungi Yeast and yeast like saprophyte dermatomycosis Yeast & yeast

More information

Fungal Infections in Neutropenic Hematological Disorders

Fungal Infections in Neutropenic Hematological Disorders Fungal Infections in Neutropenic Hematological Disorders 23 Dr Farah Jijina 24 Fungal Infections in Neutropenic Hematological Disorders 25 Dr Farah Jijina 26 Fungal Infections in Neutropenic Hematological

More information

Antifungal Susceptibility Testing

Antifungal Susceptibility Testing Infect Dis Clin N Am 20 (2006) 699 709 Antifungal Susceptibility Testing Annette W. Fothergill, MA, MBA, MT(ASCP), CLS(NCA) a, Michael G. Rinaldi, PhD a,b, Deanna A. Sutton, PhD, MT, SM(ASCP), SM, RM(NRM)

More information

2046: Fungal Infection Pre-Infusion Data

2046: Fungal Infection Pre-Infusion Data 2046: Fungal Infection Pre-Infusion Data Fungal infections are significant opportunistic infections affecting transplant patients. Because these infections are quite serious, it is important to collect

More information

NON TRADITIONAL MANAGEMENT OF FUNGAL KERATITIS

NON TRADITIONAL MANAGEMENT OF FUNGAL KERATITIS NON TRADITIONAL MANAGEMENT OF FUNGAL KERATITIS MOHAMED SAAD,MD PROF. OF OPHTHALMOLOGY ASSIUT UNIVERSITY HOSPITAL FINANCIAL DISCLOSURE: No financial interest. 1 Fungal keratitis is a sight-threatening condition

More information

MANAGEMENT OF HOSPITAL-ACQUIRED FUNGAL INFECTIONS

MANAGEMENT OF HOSPITAL-ACQUIRED FUNGAL INFECTIONS MANAGEMENT OF HOSPITAL-ACQUIRED FUNGAL INFECTIONS Paul D. Holtom, MD Associate Professor of Medicine and Orthopaedics USC Keck School of Medicine Numbers of Cases of Sepsis in the United States, According

More information

Fungal Infection in Clear Corneal Incision Wound Leads to Acute Endophthalmitis Post Phacoemulsification: A Case Series

Fungal Infection in Clear Corneal Incision Wound Leads to Acute Endophthalmitis Post Phacoemulsification: A Case Series JOURNAL OF CASE REPORTS 2014;4(2):434-442 Fungal Infection in Clear Corneal Incision Wound Leads to Acute Endophthalmitis Post Phacoemulsification: A Case Series Faresa Hilda 1, Rina La Distia Nora 2,

More information

New antifungal agents

New antifungal agents New antifungal agents Dr Atul Patel, MD, FIDSA Chief Consultant and Director Infectious Diseases Clinic Vedanta Institute of Medical Sciences Ahmedabad, India Presented at MMTN Malaysia Conference 5 6

More information

TEPZZ Z9Z74_A_T EP A1 (19) (11) EP A1 (12) EUROPEAN PATENT APPLICATION

TEPZZ Z9Z74_A_T EP A1 (19) (11) EP A1 (12) EUROPEAN PATENT APPLICATION (19) TEPZZ Z9Z74_A_T (11) EP 3 090 741 A1 (12) EUROPEAN PATENT APPLICATION (43) Date of publication: 09.11.2016 Bulletin 2016/4 (21) Application number: 1642039.1 (1) Int Cl.: A61K 31/203 (2006.01) A61K

More information

Take home messages from ARC meet

Take home messages from ARC meet Take home messages from ARC meet Prophylaxis for endophthalmitis 1) Biometry should not be done on the same day of surgery. 2) Povidone Iodine use before surgery is mandatory (preferably three minutes

More information

Voriconazole. Voriconazole VRCZ ITCZ

Voriconazole. Voriconazole VRCZ ITCZ 7 7 8 7 8 fluconazole itraconazole in vitro in vivo Candida spp. C. glabrata C. krusei Cryptococcus neoformans in vitro Aspergillus spp. in vitro in vivo Aspergillus fumigatus Candida albicans C. krusei

More information

Definition. Phaeohyphomycosis

Definition. Phaeohyphomycosis Phaeohyphomycosis Synonyms: Cerebral chromomycosis, chromoblastomycosis, chromomycosis, cladosporiosis, phaeomycotic cyst, phaeosporotrichosis, subcutaneous mycotic cyst. Definition Phaeohyphomycosis consists

More information

Post-LASIK infections

Post-LASIK infections Post-LASIK infections By Mohamed El-moddather Assiss. Prof. and head of department of ophthalmology AL-Azhar unizersity Assuit LASIK has become a common refractive procedure and is generally considered

More information

Ali Alabbadi. Sarah Jaar ... Nader

Ali Alabbadi. Sarah Jaar ... Nader 24 Ali Alabbadi Sarah Jaar... Nader Intro to Mycology *underlined text was explained in the lecture but is not found in the slides -mycology: the study of the mycoses of man (fungal infections) -less than

More information

CASE REPORT POST-OPERATIVE ENDOPHTHALMITIS DUE TO FUSARIUM DIMERUM

CASE REPORT POST-OPERATIVE ENDOPHTHALMITIS DUE TO FUSARIUM DIMERUM CASE REPORT POST-OPERATIVE ENDOPHTHALMITIS DUE TO FUSARIUM DIMERUM Sadia Khan 1, Gopal S Pillai 2, V Vivek 1, Kavitha Dinesh 1 and PM Shamsul Karim 1 1 Department of Microbiology, 2 Department of Ophthalmology,

More information

Sclerokeratoplasty David S. Chu, M.D. Cases

Sclerokeratoplasty David S. Chu, M.D. Cases Sclerokeratoplasty David S. Chu, M.D. Cases Case 1 40 year-old female from Peru presented to our Service with inflamed OS for 2 months duration. Her symptoms began as red painful OS, which progressively

More information

Management of fungal infection

Management of fungal infection Management of fungal infection HKDU symposium 17 th May 2015 Speaker: Dr. Thomas Chan MBBS (Hons), MRCP, FHKCP, FHKAM Synopsis Infection caused by fungus mycoses Skin infection by fungus is common in general

More information

Nasreen A. Syed, MD F.C. Blodi Eye Pathology Laboratory University of Iowa

Nasreen A. Syed, MD F.C. Blodi Eye Pathology Laboratory University of Iowa Nasreen A. Syed, MD F.C. Blodi Eye Pathology Laboratory University of Iowa No financial interest in any of the material discussed in this presentation There will be discussion of off label use of medications,

More information

HAEMATOLOGY ANTIFUNGAL POLICY

HAEMATOLOGY ANTIFUNGAL POLICY HAEMATOLOGY ANTIFUNGAL POLICY PROPHYLAXIS Primary Prophylaxis Patient Group Patients receiving intensive remissioninduction chemotherapy for Acute Leukaemia (excluding patients receiving vinca alkaloids)

More information

Evaluation of Agent and Host Factors in Progression of Mycotic Keratitis

Evaluation of Agent and Host Factors in Progression of Mycotic Keratitis Evaluation of Agent and Host Factors in Progression of Mycotic Keratitis A Histologic and Microbiologic Study of 167 Corneal Buttons Geeta Kashyap Vemuganti, MD, 1 Prashant Garg, MS, 2 Usha Gopinathan,

More information

Common Etiological Agents Causing Keratitis: A Study from a Tertiary Care Hospital in South India

Common Etiological Agents Causing Keratitis: A Study from a Tertiary Care Hospital in South India International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 7 (2017) pp. 1625-1633 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.607.196

More information

Fungal infections in ICU. Tang Swee Fong Department of Paediatrics Universiti Kebangsaan Malaysia

Fungal infections in ICU. Tang Swee Fong Department of Paediatrics Universiti Kebangsaan Malaysia Fungal infections in ICU Tang Swee Fong Department of Paediatrics Universiti Kebangsaan Malaysia Epidemiology of invasive fungal infections - US +300% Martin GS, et al. N Engl J Med 2003;348:1546-1554

More information

Common Fungi. Catherine Diamond MD MPH

Common Fungi. Catherine Diamond MD MPH Common Fungi Catherine Diamond MD MPH Birth Month and Day & Last Four Digits of Your Cell Phone # BEFORE: http://tinyurl.com/kvfy3ts AFTER: http://tinyurl.com/lc4dzwr Clinically Common Fungi Yeast Mold

More information

Mucormycosis & pythiosis new insights

Mucormycosis & pythiosis new insights Mucormycosis & pythiosis new insights Dr Ariya Chindamporn Associate Professor Department of Microbiology Faculty of Medicine, Chulalongkorn University Bangkok, Thailand Presented at MMTN Vietnam Conference

More information

Clinico-Epidemological Profile of Corneal Ulcer Cases from Rural Hospital of Haryana, India

Clinico-Epidemological Profile of Corneal Ulcer Cases from Rural Hospital of Haryana, India International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 9 (2017) pp. 2410-2416 Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2017.609.296

More information

Clinical Study Clinical Characteristics of Alternaria Keratitis

Clinical Study Clinical Characteristics of Alternaria Keratitis Ophthalmology, Article ID 536985, 7 pages http://dx.doi.org/10.1155/2014/536985 Clinical Study Clinical Characteristics of Alternaria Keratitis Ching-Hsi Hsiao, 1,2 Lung-Kun Yeh, 1,2 Hung-Chi Chen, 1,2

More information

Dimitrios P. Kontoyiannis, MD, ScD. Frances King Black Endowed Professor

Dimitrios P. Kontoyiannis, MD, ScD. Frances King Black Endowed Professor Dimitrios P. Kontoyiannis, MD, ScD Frances King Black Endowed Professor Disclosures Research support from Merck, Pfizer and Astellas and has served as speaker for Gilead/Merck, Mylan, Inc and as consultant

More information

Pharmacy and Pharmaceutical Sciences, Monash University, 381 Royal Parade,

Pharmacy and Pharmaceutical Sciences, Monash University, 381 Royal Parade, AAC Accepts, published online ahead of print on 24 January 2011 Antimicrob. Agents Chemother. doi:10.1128/aac.01175-10 Copyright 2011, American Society for Microbiology and/or the Listed Authors/Institutions.

More information

ANTIMYCOTIC DRUGS Modes of Action

ANTIMYCOTIC DRUGS Modes of Action ANTIMYCOTIC DRUGS Modes of Action Prapasarakul Nuvee, D.V.M., Ph.D. Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University 1 What drugs act as antifungal agents?

More information

APPLICATION TO ADD NATAMYCIN TO THE ESSENTIAL LIST OF MEDICINES FOR TREATMENT OF FUNGAL KERATITIS

APPLICATION TO ADD NATAMYCIN TO THE ESSENTIAL LIST OF MEDICINES FOR TREATMENT OF FUNGAL KERATITIS APPLICATION TO ADD NATAMYCIN TO THE ESSENTIAL LIST OF MEDICINES FOR TREATMENT OF FUNGAL KERATITIS 1. Table of Contents 1. Table of Contents... 2 2. Summary statement of the proposal for inclusion, change

More information

Acanthameba Keratitis

Acanthameba Keratitis Acanthameba Keratitis CHARALAMBOS S. SIGANOS, MD, PHD ASSOC. PROFESSOR OF OPHTHALMOLOGY UNIVERSITY OF CRETE DEPARTMENT OF OPHTHALMOLOGY HERAKLION UNIVERSITY HOSPITAL CRETE GREECE I declare no conflict

More information

M Rezanur Rahman, Gordon J Johnson, Rabiul Husain, Shahe A Howlader, Darwin C Minassian

M Rezanur Rahman, Gordon J Johnson, Rabiul Husain, Shahe A Howlader, Darwin C Minassian Br J Ophthalmol 1998;82:919 925 919 Institute of Ophthalmology, Department of Preventive Ophthalmology, University College London, Bath Street, London, EC1V 9EL M R Rahman G J Johnson D C Minassian Eye

More information

How to make a fast diagnosis

How to make a fast diagnosis How to make a fast diagnosis 8 th Advances Against Aspergillosis 1 3 February 2018 Lisbon, Portugal Cornelia Lass-Flörl Division of Hygiene and Medical Microbiology Innsbruck Medical University Division

More information

Voriconazole October 2015 Risk Management Plan. Voriconazole

Voriconazole October 2015 Risk Management Plan. Voriconazole Voriconazole October 2015 VI.2 VI.2.1 Elements for a Public Summary Overview of disease epidemiology Invasive aspergillosis (IA) is the most devastating of Aspergillus related diseases, targeting severely

More information

Challenges and controversies of Invasive fungal Infections

Challenges and controversies of Invasive fungal Infections Challenges and controversies of Invasive fungal Infections Mona Al-Dabbagh, MD, MHSc Assistant Professor of Pediatrics, COM-KSAU-HS Consultant Pediatric Infectious Diseases and Transplant Infectious Diseases

More information

CD101: A Novel Echinocandin

CD101: A Novel Echinocandin CD101: A Novel Echinocandin Taylor Sandison, MD MPH Chief Medical Officer TIMM Belgrade, Serbia October 8, 2017 1 Disclosures Dr. Sandison is an employee of and stockholder in Cidara Therapeutics 2 Cidara

More information

Acanthamoeba Keratitis in a Non-contact Lens Wearer: A Challenge in Diagnosis and Management

Acanthamoeba Keratitis in a Non-contact Lens Wearer: A Challenge in Diagnosis and Management JOURNAL OF CASE REPORTS 2014;4(2):419-423 Acanthamoeba Keratitis in a Non-contact Lens Wearer: A Challenge in Diagnosis and Management Dian Eka Putri, Lukman Edwar, Made Susiyanti Department of Ophthalmology,

More information

In Vitro Comparison of Efficacy of Natamycin and Silver Nitrate against Ocular Fungi ACCEPTED

In Vitro Comparison of Efficacy of Natamycin and Silver Nitrate against Ocular Fungi ACCEPTED AAC Accepts, published online ahead of print on 12 January 2009 Antimicrob. Agents Chemother. doi:10.1128/aac.00697-08 Copyright 2009, American Society for Microbiology and/or the Listed Authors/Institutions.

More information

Ana Espinel-Ingroff 1, Elizabeth Johnson 2, Hans Hockey 3 and Peter Troke 4 *

Ana Espinel-Ingroff 1, Elizabeth Johnson 2, Hans Hockey 3 and Peter Troke 4 * Journal of Antimicrobial Chemotherapy (2008) 61, 616 620 doi:10.1093/jac/dkm518 Advance Access publication 25 January 2008 Activities of voriconazole, itraconazole and amphotericin B in vitro against 590

More information

Newer Combination Therapies

Newer Combination Therapies Newer Combination Therapies William J. Steinbach, MD Associate Professor of Pediatrics, Molecular Genetics & Microbiology Pediatric Infectious Diseases Duke University Medical Center Combination Therapy

More information

on November 3, 2018 by guest

on November 3, 2018 by guest JOURNAL OF CLINICAL MICROBIOLOGY, June 2007, p. 1811 1820 Vol. 45, No. 6 0095-1137/07/$08.00 0 doi:10.1128/jcm.00134-07 Copyright 2007, American Society for Microbiology. All Rights Reserved. Multicenter

More information

In vivo response in a mixed infection model of azole susceptible and resistant Aspergillus spp. strains Laura Alcazar-Fuoli, CNM, ISCIII

In vivo response in a mixed infection model of azole susceptible and resistant Aspergillus spp. strains Laura Alcazar-Fuoli, CNM, ISCIII In vivo response in a mixed infection model of azole susceptible and resistant Aspergillus spp. strains Laura Alcazar-Fuoli, CNM, ISCIII Pre-Advances Against Aspergillosis Workshop: Aspergillus Speciation

More information

We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists. International authors and editors

We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists. International authors and editors We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists 4,000 116,000 120M Open access books available International authors and editors Downloads Our

More information

ETIOLOGY OF SUPPURATIVE CORNEAL ULCERS IN RURAL POPULATION OF NORTHERN INDIA

ETIOLOGY OF SUPPURATIVE CORNEAL ULCERS IN RURAL POPULATION OF NORTHERN INDIA RESEARCH ARTICLE ETIOLOGY OF SUPPURATIVE CORNEAL ULCERS IN RURAL POPULATION OF NORTHERN INDIA Sushil Ojha 1, *, Anupama Tandon 2, Dipendra Shukla 3, Neeraj Saraswat 4, Shweta Joshi 5 1 Senior Resident,

More information