Vol. 41 No Journal of Jiangxi Normal University Natural Science Sep. 2017
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1 41 5 Vol 41 No Journal of Jiangxi Normal University Natural Science Sep II * II ELISA IgE IgG IL-6 TNF-α IFN-γ 5 10 mg kg - 1 II 10 mg kg - 1 IgE IgG IL-6 TNF-a IFN-r 10 mg kg mg kg - 1 II II R A DOI /j cnki issn RA Ⅱ 9 rheumatoid arthritis RA 1-2 II RA RA 3 RA % ~ 0 36% wistar 32 SPF 4 ~ ± 15 g 5 RA sigma LPS sigma II RA IgG IgE ELISA JCYJ lzg195910@ 126 com
2 5 II 517 ebioscience IL-6 TNF-α IFN-γ ELISA CCl II IgG sigma IgE 1 2 ELISA II 5 0 g II IgG IgE 10 4 mol L IL-6 TNF-α IFN-γ 24 h r min min ELISA mol IL-4 TNF-α IFN-γ L g L h r min min mol L - 1 ph mol L - 1 tris-hcl 0 20 mol L - 1 NaCl ph μm HE SDS II ± s Wistar 4 8 / CCl 4 + LPS + II M1 5 mg kg II + CCl 4 + LPS M2 10 mg kg II 2 II + CCl 4 + LPS M M2 M mg kg mm SPSS 16 0 t 2 SDS- II kda II sigma II II 1 M M1 M2 M3 10 mg II M1 M2 M3 0 1 ml CCl M1 M2 M mg M 1 II 2 sigma II 0 ~ II
3 p < M2 8 M2 M3 0 8 M1 M1 M ± ± ± ± M ± ± ± ± M ± ± ± 0 01 * 0 55 ± 0 04 * 0 8 * M ± ± 0 03 * 0 50 ± 0 04 * 0 59 ± 0 06 ** 2 6 ** M1 CCl 4 + LPS + II M2 5 mg kg - 1 II + CCl 4 + LPS M3 10 mg kg - 1 II + CCl 4 + LPS 2 3 II IgE IgG ELISA II IgE IgG 2 M2 M3 II IgE IgG p < 0 05 M3 IgE IgG M1 p > 0 05 * p < 0 05 ** p < IL-6 IFN-γ TNF-a M1 * p < 0 05 ** p < II IgE IgG 2 4 ELISA IL-6 IFN-γ TNF-α 3 M1 M2 IFN-γ 10 mg kg - 1 M3 IFN-γ p < 0 05 M1 IL-6 TNF-α p > 0 05 M2 M3 4 A 4 B M1 CCl 4 + LPS + II 4 C M2 5 mg kg - 1 II + CCl 4 + LPS IL-6 TNF-α p < D M3 10 mg kg - 1 II 10 mg kg - 1 M3 + CCl 4 + LPS p < 0 01 II 4 HE
4 5 II 519 M2 2 J M J 3 5 Zhangpeng Hu Yiping Li Jinchao et al Study in treatment of collagen-induced arthritis in DBA /1 mice model RA by genistein J Curr Pharm Des RA IgG IgE J TH2 IL-6 IL-4 J TNF-α RA IgA Ara h2 J RA RA 15 J RA Wu Dandan Liu Xiaoyu Su Hong et al Oral antigens induce rheumatoid arthritis-like inflammation in a rat model J Inflamm Res II RA 11 Vaghef-Mehrabany E Vaghef-Mehrabany L Asghari-Jafarabadi M et al Effects of probiotic supplementation on II lipid profile of women with rheumatoid arthritis A randomized placebo-controlled clinical trial J Health Promot 5 mg kg - 1 Perspect RA 12 Marietta E V Murray J A Luckey D H et al Suppression IgE IgG of inflammatory arthritis by human gut-derived prevotella IL-6 TNF-α IFN-γ NF-κB J II 14 IL-23 /IL-17 RA J J J histicola in humanized mice J Arthritis Rheumatol 15 Ⅱ J
5 The Establishment and Accessing of Rheumatoid Arthritis Model in Rat by Oral Bovine Collagen Type II GUAN Li 1 WU Dandan 2 HU Dongsheng 2 LIU Dongzhou 3 LIU Zhigang 1 2* 1 Department of Rheumatology Luohu People's Hospital Shenzhen Guangdong China 2 Institute of Allergy and Immunology School of Medicine Shenzhen University Shenzhen Guangdong China 3 Department of Rheumatology Shenzhen People's Hospital Shenzhen Guangdong China Abstract An RA animal model was developed by gavage feeding with bovine collagen type II and aspirin and tail vein injection with lipopolysaccharide The diameter of ankle were measured and the score of the arthritis The concentration of IgE IgG IL-6 TNF-α and IFN-γ in serum by ELISA test The inflammation in the ankle joints was evaluated by light microscopy and the ankle histopathological changes The results show that Rats treated with high doses of aspirin 10 mg kg - 1 can be successfully induced by oral bovine collagen type II The ankle of rat presents obvious swelling and arthritis symptoms High levels of proinflammatory cytokines and specific antibodies were detected in the sera The histology showed an inflammatory feature in the ankle joints Rats treated with high doses of aspirin 10 mg kg - 1 can be successfully induced by oral bovine collagen type II Key words rheumatoid arthritis food antigen oral bovine collagen type II animal model 515 The Analysis on Ecological Habits of Common Birds in Electric Transmission Lines from Jiangxi Province LI Yanglin 1 XU Ning 1 ZHANG Yu 1 ZHI Yijin 2 SHAO Mingqin 2* 1 Jiangxi Electric Power Research Institute of China Power Grid Nanchang Jiangxi China 2 College of Life Science Jiangxi Normal University Nanchang Jiangxi China Abstract The species quantity and ecological habits of birds on the earth wire and iron towers of electric transmission lines in Jiangxi province are investigated using line transects and point counts methods combined with a random survey from November 2011 to May 2017 Forty-nine species of birds are recorded on the earth wire or iron towers including two Chinese first key protected animals and three Chinese second key protected animals There are 20 species of mainly medium and small-sized birds with higher frequency in the earth wire and iron towers Birds have diverse ecological habits and similar habitats such as hills forests and agricultural land The results showe that Sturnus nigricollis Sturnus sericeus and Ciconia boyciana have more probability of causing transmission lines breakdown Resident is the main element of birds during this study as for the fauna the birds of Oriental realm has the highest proportion Birds with low population size and high endangered rank always have higher diffusion capacity Several suggestions on how to reduce electric transmission line breakdown are discussed Key words Jiangxi Province electric transmission line birds habit
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