Development and evaluation of user-developed protocols for bacterial respiratory pathogens leveraging the snap-load-go workflow of BD MAX TM
|
|
- Britton Bryan
- 6 years ago
- Views:
Transcription
1 PAMM FOUNDATION LABORATORY FOR PATHOLOGY AND MEDICAL MICROBIOLOGY Development and evaluation of user-developed protocols for bacterial respiratory pathogens leveraging the snap-load-go workflow of BD MAX TM Ingrid Op den Buijs, Senior technologist PAMM, Laboratory for Medical Microbiology, The Netherlands
2 PAMM Laboratories, Veldhoven, The Netherlands Google Maps, 2013
3 Laboratory for Clinical Microbiology, Veldhoven, The Netherlands
4 Public Health Service regions The PAMM public laboratory of medical microbiology has an adherence area of 800,000 people in South-East Brabant. It serves four hospitals, nursing homes and 400 general practitioners. 137,000 molecular diagnostic (real-time PCR) tests in 2014 at the laboratory of medical microbiology of those were on the BD MAX
5 Molecular PAMM Respiratory infections Chlamydophila pneumoniae BD MAX Mycoplasma pneumoniae BD MAX Legionella pneumophilla BD MAX Chlamydophila psittaci BD MAX Mycobacterium tuberculosis complex Bordetella pertussis Bordetella parapertussis Pneumocystis carnii pneumonia Influenza A/B Respiratory syncytial virus (RSV) Rhinovirus Other Coxiella burnetti Streptococcus pneumoniae Dermatophytes Mumps Infection prevention ESBL Carbapenemases VRE MRSA STD Neisseria gonorrhoeae Chlamydia trachomatis Trichomonas vaginalis Mycoplasma genitalium Chlamydia trachomatis LGV CSF Herpes simplex virus 1/2 VZV Enterovirus Parechovirus Gastro-enteritis Salmonella spp Shigella spp Yersinia spp Campylobacter jejuni/lari/coli STEC Giardia lamblia Cryptosporidium parvum Dientamoeba fragilis Entamoeba histolytica Norovirus GI/GII BD MAX Rotavirus - BD MAX C.difficile toxins BD MAX Sequencing 16S sequencing LSU sequencing Next generation sequencing
6 Pre-BD MAX era at PAMM In-house developed assays easymag extraction followed by qpcr on ABI7500fast system Twice a week about 20 requests for qpcr bacterial respiratory pathogens: Mycobacterium tuberculosis complex Legionella pneumophila Mycoplasma pneumoniae Chlamydophila pneumoniae Chlamydophila psittaci
7 Migration to BD MAX Time-line (1-4 samples) BD MAX EasyMAG-ABI Sample prep Hands-on Extraction Hands-on2 PCR 00:00 00:14 00:28 00:43 00:57 01:12 01:26 01:40 01:55 02:09 02:24
8 BD MAX Tree different protocols: Closed BD IVD assays (CDIFF, Staph SR) Open BD mastermix, in-house primers and probes Research in-house primers, probes and mastermix
9 Leveraging the snap load go workflow Utilisation of dried primer-probe snap-ins to go with DNA kits
10 Migration b-cap to BD MAX Bacteria Target gene Sensitivity 1 QCMD12 L. pneumophila Outer membrane protein A 75 cp/ml 100% M. pneumoniae Macrophage infectivity potentiator protein 230 cp/ml 100% core 2 C. pneumoniae Outer membrane protein A 110 cp/ml 100% C. psittaci P1 adhesion protein gene 40 cp/ml 100% 3 1 Based on 500µl input. 2 One educational sample was not detected by the bcap assay (detected positive by 22.5% of participants). 3 For C.psittaci the 2013 panel was tested.
11 Experience b-cap Introduced to the routine-lab since october 1st 280 samples were tested 12 M.pneumoniae positives 4 L.pneumophila positives No C.psittaci or C.pneumoniae positives Due to easy of use: 7 days/week oct-2014 nov-14 dec-14 jan-15 feb-15 Lpn Cps Cpn Mpn negatives
12 N M.tuberculosis complex diagnostics Total of 1891 TB culture requests in samples were also tested in PCR. Mean of 28 requests for PCR a week. Prevalence of 1.5% TB complex positive samples (23 positive patients) PCR positive PCR negative Week number
13 Multicenter study Collaboration of PAMM (Veldhoven), USC (Santiago de Compostella) & NHS Lothian (Edinburgh) External quality assessment: QCMD panel 2012 Analytical sensitivity with positive plasmid control Different pre-treatments Extended clinical evaluation
14 QCMD 2012 All sites ordered the QCMD 2012 panel at Qnostics Samples were proteinase K treated PAMM NHS Lothian USC QCMD Core? Sample Sample type MTB IC MTB IC MTB IC cells/vial MTB12-01 PRB Y MTB12-02 PRB N MTB12-03 PRB Y MTB12-04 PRB Y MTB12-05 PRB Y MTB12-06 Sputum Y MTB12-07 Sputum Y MTB12-08 Sputum Y MTB12-09 Sputum Y MTB12-10 Sputum N All sites score 100%
15 Limit of detection Triplicates of IS6110 target-containing plasmids. Ten-fold dilutions tested in triplicates at all sites. The limit of detection was determined at cp/ml at all laboratory sites. Further experiments showed a LoD of 270 cp/ml (13-54 genome copies). Copies IS6110 per genome Concept Dutch NvMM guidelines MTB
16 Pre-treatment of clinical specimens Tested both sputum as BAL samples. Pre-treatment methods that were used: Sputasol followed by 2% NaOH-NALC solution and phosphate buffer; BD Mycoprep decontamination treatment; Proteinase K incubation. After pre-treatment samples boiled to inactivate the MTB. Experiences with pre-treatments varies per laboratory. Most important is to liquefy the sample for pipetting purposes. One pre-treatment that worked for all participating laboratories: BD Mycoprep
17 Clinical evaluation Retrospective evaluation Pre-treatment methods customary for the specific laboratory sites: Sputasol followed by 2% NaOH-NALC solution and phosphate buffer; BD Mycoprep decontamination treatment; Proteinase K incubation. Prospective evaluation All the same treatmentmethod: BD Mycoprep Gold standard BBL MGIT source: BD.com Results were compared to smear results and culture A sample from a patient with proven active MTB was defined as true positive
18 Retrospective vs prospective study Culture Retrospective pos neg pos BDmax neg Sensitivity 96.3% Specificity 98.5% PPV 96.3% NPV 98.5% Culture Prospective pos neg pos BDmax neg Sensitivity 81.7% Specificity 95.0% PPV 80.3% NPV 95.4%
19 Retrospective vs prospective study True Retrospective pos neg pos BDmax neg Sensitivity 96.4% Specificity 100.0% PPV 100.0% NPV 98.5% True Prospective pos neg pos BDmax neg Sensitivity 84.7% Specificity 100.0% PPV 100.0% NPV 95.4%
20 Results clinical evaluation Retrospectively 25% of true positives were smear-microscopy negative All smear-microscopy positives were detected positive in qpcr Three (1%) of true positive samples not detected in qpcr From one patient 3 other samples were detected positive in qpcr All were smear-microscopy negative Prospectively 42% of true positives were smear-microscopy negative All smear-microscopy positives were detected positive in qpcr Eleven (3.7%) of true positive samples not detected in qpcr Eight different patients From four patients 1 other sample was detected positive in qpcr From the other four patients no other samples were tested All were smear-microscopy negative
21 Conclusion MTB BD MAX MTB assay is a sensitive and specific screening method of the detection of M.tuberculosis complex. The assay could provide a rapid alternative to smear-microscopy
22 Soon commercially available at Biolegio Detection of M.tuberculosis complex Quality control testing is ongoing Expected release-date: mid April 2015 Information & protocol ReadyMax TM snap-ins
23 Thanks to.. Jeroen van de Bovenkamp René Roymans Ingrid Op den Buijs Dominique Clarysse Ruud van der Steen Frank Spijkers Suzanne Paule
24
PATHOGEN DETECTION WITH THE FILMARRAY
PATHOGEN DETECTION WITH THE FILMARRAY The System Sample-to-Answer in an Hour Single sample Multiple samples The FilmArray integrates sample preparation, amplification, detection, and analysis all into
More informationM O L E C U L A R G E N E T I C S
MOLECULAR GENETICS ADVANTAGES OF MOLECULAR GENETICS Molecular genetics is a dynamic and transformative area of diagnostics, leading to insights in research and treatment in many disease states that are
More informationNeonatal Pneumonia-2 assay
Neonatal Pneumonia-2 assay For the detection of Ureaplasma species (Ureaplasma parvum and Ureaplasma urealyticum), Chlamydia trachomatis and Mycoplasma hominis using the BD MAX TM system. Instructions
More informationAMPLIRUN TOTAL A RELIABLE QUALITY CONTROL SOURCE FOR NUCLEIC ACID TESTS
AMPLIRUN TOTAL A RELIABLE QUALITY CONTROL SOURCE FOR NUCLEIC ACID TESTS Take Control, Molecular Control. 02 04 05 06 06 07 08 08 AMPLIRUN TOTAL RESPIRATORY INFECTIONS TUBERCULOSIS INFECTIONS GASTROINTESTINAL
More informationPotential Reimbursement CPT Codes
BioFire FilmArray Blood Culture Identification (BCID) Panel Medicare All targets (n) 87150 n x * *BioFire BCID Panel is comprised of 27 total targets. The number of targets allowed for reimbursement may
More informationCollection (Specimen Source Required on all tests) Sputum: >5 ml required. First morning specimen preferred.
Type Acid Fast (Mycobacteria) Sputum: >5 ml required. First morning specimen preferred. For blood, sodium heparin tube preferred. Lithium heparin acceptable. Do not centrifuge.. delay. Swabs are not appropriate
More informationINFECTIOUS DISEASE. Page 2
Infectious disease Advantages OF TESTING INFECTIOUS DISEASE We are in the middle of a paradigm shift in infectious disease diagnostic testing. As we move from targeted infectious disease testing to a syndromic
More informationMicrobiology EQA Product Portfolio
Labquality EQAS Microbiology EQA Product Portfolio Clinically relevant external quality assessment program for microbiology Bacterial serology Bacteriology Mycology Parasitology Preanalytics Virology Labquality
More informationClinical Microbiology CLS 2019 Clinical Microbiology Laboratory Scheme Application Form
Laboratory Scheme Application Form complete all sections below and return to LGC Standards Proficiency Testing by email, fax or post. Returning customer Lab ID: Purchase order no.: (compulsory) Round Despatch
More informationDEPARTMENT OF MICROBIOLOGY IMPORTANT NOTICE TO USERS Turnaround Times (TATs) for Microbiology Investigations
Dear User, ISSUE: M008 DEPARTMENT OF MICROBIOLOGY IMPORTANT NOTICE TO USERS Turnaround Times (TATs) for Microbiology Investigations In order to comply with national quality guidance and as part of our
More informationProgramme Groupings. For 2013, the preliminary molecular external quality assessment (EQA) programme schedule has been displayed by Disease Group.
Equalis EQA s 2013 Preliminary Schedule For 2013, the preliminary molecular external quality assessment (EQA) programme schedule has been displayed by Disease Group. Note: EQA programmes may appear in
More information3base The Science Behind EasyScreen Molecular Diagnostic Assays. Nucleic Acid Conversion Technology
3base The Science Behind EasyScreen Molecular Diagnostic Assays Nucleic Acid Conversion Technology 1 Topics 3base Technology Overview Technical Advantages of 3base Validation of 3base Specificity Validation
More informationScheme Description. Clinical Microbiology Laboratory Scheme
CLS Clinical Microbiology Laboratory Scheme Scheme Description LGC Standards Proficiency Testing 1 Chamberhall Business Park Chamberhall Green Bury Lancashire BL9 0AP United Kingdom Telephone: +44 (0)
More informationMENU PRODUCT MOLECULAR. International Product Listing. Simplexa Molecular Kits Integrated Cycler Molecular Reagents and Primer Pairs
International Product Listing MOLECULAR PRODUCT MENU Simplexa Molecular Kits Integrated Cycler Molecular Reagents and Primer Pairs Products are not intended for donor screening. Simplexa Molecular Kits
More informationPAMET Continuing Education 2016
PAMET Continuing Education 2016 Agent of gastroenteritis Medium/method] used for routine screening/detection in stool samples Salmonella, Shigella, MacConkey, Hektoen, Bismuth sulfite,etc. Plesiomonas
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Clinical Microbiology QMC Campus Derby Road Nottingham NG7 2UH Contact: Jil Bowskill Tel: +44 (0) 1905 760192 Ext 30659 E-Mail: jilean.bowskill@nuh.nhs.uk
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Microbiology Department Contact: Dr David Ashburn Zone 3 Raigmore Hospital Tel: +44 (0) 1463 704108 Old Perth Road Fax: +44 (0) 1463 705648
More informationValidation FTD Respiratory pathogens 33
Validation FTD Respiratory pathogens 33 32 reactions (catalog no. FTD-2P.3-32) 64 reactions (catalog no. FTD-2P.3-64) Qualitative assay for in vitro diagnostics Validated with: Applied Biosystems 7500
More informationQUALITY CONTROL for MOLECULAR DIAGNOSTICS Quality issues highlighted through international external quality assessment
QUALITY CONTROL for MOLECULAR DIAGNOSTICS Quality issues highlighted through international external quality assessment William G MacKay PhD Neutral Office Coordinator QCMD The Altum Building, Todd Campus
More informationThe Cost-effectiveness of a GI PCR panel in Detecting Necessary to Treat Infections
The Cost-effectiveness of a GI PCR panel in Detecting Necessary to Treat Infections Annie L. Andrews MD, MSCR Annie N. Simpson PhD Kit N. Simpson DrPH Daniel C. Williams MD, MSCR The authors have nothing
More informationA New Multiplex Real-time PCR Assay For Detection Of Intestinal Parasites
A New Multiplex Real-time PCR Assay For Detection Of Intestinal Parasites Dr. Andreas Simons Worldwide provider of diagnostic assay solutions Offers a variety of test kit methodologies R-Biopharm Headquarters
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Royal Liverpool & Broadgreen University Hospitals NHS Trust Department of Virology Liverpool Clinical Laboratories Royal Liverpool and Broadgreen
More informationBactoReal Kits for detection of bacteria
BactoReal Kits for detection of bacteria BactoReal Kit Bartonella spp. Detection of the glta gene of B. clarridgeiae, B. elizabethae, B. grahamii, B. henselae, B. koehlerae, B. quintana, B. volans and
More informationCorporate Medical Policy
Corporate Medical Policy Identification of Microorganisms Using Nucleic Acid Probes File Name: Origination: Last CAP Review: Next CAP Review: Last Review: identification_of_microorganisms_using_nucleic_acid_probes
More informationChlamydia MIF IgG. Performance Characteristics. Product Code IF1250G Rev. J. Not for Distribution in the United States
Product Code IF1250G Rev. J Performance Characteristics Not for Distribution in the United States EXPECTED VALUES Community Acquired Pneumonia Population Two outside investigators assessed the Focus Chlamydia
More informationMICROBIOLOGY SPECIMEN COLLECTION MANUAL
Lee Memorial Health System Lee County, FL CLINICAL LABORATORY MICROBIOLOGY SPECIMEN COLLECTION MANUAL ACID FAST CULTURE Specimen Type see Specimen Chart ACID FAST STAIN see Specimen Chart Acid Fast stain
More informationTest Requested Specimen Ordering Recommendations
Microbiology Essentials Culture and Sensitivity (C&S) Urine C&S Catheter Surgical (excluding kidney aspirates) Voided Requisition requirements o Specific method of collection MUST be indicated o Indicate
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Microbiology Department Crawley Hospital West Green Drive Crawley RH11 7DH United Kingdom Contact: Clare Reynolds Tel: +44 (0) 1293 600379
More informationAttendees will understand the early experience and clinical impact of GI multiplex PCR diagnostics in children
Participants will understand the role of a comprehensive business case in considering the introduction of novel technology affecting multiple areas of the laboratory Attendees will learn about the impact
More informationPneumonia Aetiology Why is it so difficult to distinguish pathogens from innocent bystanders?
Pneumonia Aetiology Why is it so difficult to distinguish pathogens from innocent bystanders? David Murdoch Department of Pathology University of Otago, Christchurch Outline Background Diagnostic challenges
More informationMICROBIOLOGICAL TESTING IN PICU
MICROBIOLOGICAL TESTING IN PICU This is a guideline for the taking of microbiological samples in PICU to diagnose or exclude infection. The diagnosis of infection requires: Ruling out non-infectious causes
More informationHPSC - Weekly Infectious Disease Report
HPSC - Weekly Infectious Disease Report Statutory Notifications of Infectious Diseases reported in Ireland via the Computerised Infectious Disease Reporting (CIDR) system for: Week 39, 2018 (Notification
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
Issue No: 001 Issue date: 28 September 2018 Department of Microbiology Contact: Diane Matley Level B Tel: +44 (0)23 8120 6408 Pathology Block E-Mail: Diane.Matley@uhs.nhs.uk Southampton General Hospital
More informationSection 1 has been repealed by Decree of 30 December 2003/1383.
Ministry of Social Affairs and Health, Finland Unofficial translation No. 786/1986 Communicable Diseases Decree Issued on 31 October 1986 Section 1 has been repealed by Decree of 30 December 2003/1383.
More informationRespiratory Pathogen Panel TEM-PCR Test Code:
Respiratory Pathogen Panel TEM-PCR Test Code: 220000 Tests in this Panel Enterovirus group Human bocavirus Human coronavirus (4 types) Human metapneumovirus Influenza A - Human influenza Influenza A -
More informationBorrelia burgdorferi sensu lato complex Assay
Borrelia burgdorferi sensu lato complex Assay For the detection of Borrelia burgdorferi sensu lato complex (ospa- and p41(flagellin) genes) using the BD MAX TM system. Instructions for use (Version 1.0
More informationInfectious Disease Antibodies and Antigens. ...Viro Stat. Supplying Researchers and Manufacturers Since
Infectious Disease Antibodies and Antigens...Viro Stat Supplying Researchers and Manufacturers Since 1985 Infectious Disease Antibodies and Antigens...Viro Stat Supplying Researchers and Manufacturers
More informationHPSC - Weekly Infectious Disease Report
HPSC - Weekly Infectious Disease Report Statutory Notifications of Infectious Diseases reported in Ireland via the Computerised Infectious Disease Reporting (CIDR) system for: Week 49, 2018 (Notification
More informationHPSC - Weekly Infectious Disease Report
HPSC - Weekly Infectious Disease Report Statutory Notifications of Infectious Diseases reported in Ireland via the Computerised Infectious Disease Reporting (CIDR) system for: Week 33, 2018 (Notification
More informationHPSC - Weekly Infectious Disease Report
HPSC - Weekly Infectious Disease Report Statutory Notifications of Infectious Diseases reported in Ireland via the Computerised Infectious Disease Reporting (CIDR) system for: Week 38, 2018 (Notification
More informationHPSC - Weekly Infectious Disease Report
HPSC - Weekly Infectious Disease Report Statutory Notifications of Infectious Diseases reported in Ireland via the Computerised Infectious Disease Reporting (CIDR) system for: Week 50, 2018 (Notification
More informationHPSC - Weekly Infectious Disease Report
HPSC - Weekly Infectious Disease Report Statutory Notifications of Infectious Diseases reported in Ireland via the Computerised Infectious Disease Reporting (CIDR) system for: Week 3, 2019 (Notification
More informationHPSC - Weekly Infectious Disease Report
HPSC - Weekly Infectious Disease Report Statutory Notifications of Infectious Diseases reported in Ireland via the Computerised Infectious Disease Reporting (CIDR) system for: Week 22, 2018 (Notification
More informationStorage conditions. roomtemperature or incubator at 36 C. Other: 2-8 C Blood: roomtemperature or incubator at. A.s.a.p. max 24h. A.s.a.p.
Extract Vademecum Department of Medical Microbiology Status: Code: Version: Approval date: Geldig MMB-PR-04/1 1 Juni 2017 Name request Specimen Material needed Transport Bacteriology Bacterial culture
More informationCollection Container Category Source Tests Order Codes
Nasal Nares culture for MRSA screen ONLY (Outpatient) NSL Room BD BBL Dual Culture Swab (Red Cap) Ear Ear culture EARC Room Eye Eye Culture EYEC Room Cultures Vaginal Genital Culture GEN or GC Room Superficial
More informationPEACEHEALTH LABORATORIES
360-414-2306 www.peacehealthlabs.org Critical Values Call List - Longview Critical values are reported per the criteria published below. Laboratory results meeting these criteria indicate potential life-threatening
More informationThe Netherlands Donor Feces Bank a. it takes stool to get better
The Netherlands Donor Feces Bank a it takes stool to get better H.W. Verspaget Dept. of Biobanking and Dept. of Gastroenterology Leiden University Medical Center The Netherlands Fecal MicrobiotaTransplantation
More informationChlamydia MIF IgM. Performance Characteristics. Product Code IF1250M Rev. I. Not for Distribution in the United States
Product Code IF1250M Rev. I Performance Characteristics Not for Distribution in the United States EXPECTED VALUES Community Acquired Pneumonia Population Two outside investigators assessed the Focus Chlamydia
More informationVersion number CAT2018/02
www.qcmd.org Version number CAT2018/02 Contents An Introduction to the QCMD EQA Schemes 5 EQA Groups Blood Borne Virus 6 Central Nervous System 6 Congenital Infections 7 Drug Resistance 7 Exotic/Emerging
More informationNew Molecular Diagnostic Assays for Solana Performance Assessment, Workflow Analysis, and Clinical Utility
New Molecular Diagnostic Assays for Solana Performance Assessment, Workflow Analysis, and Clinical Utility Gerald A. Capraro, Ph.D., D(ABMM) Director, Clinical Microbiology Carolinas Pathology Group Atrium
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
Issue No: 001 24 September 2018 Department of Microbiology Longfleet Road Poole Dorset BH15 2JB Contact: Andrew Barber Tel: +44 (0)202 448585 E-Mail: andy.barber@poole.nhs.uk Website: www.poole.nhs.uk
More informationMicrobiology & Virology Resource Comprehensive
Abscess, aspirate, drainage culture Actinomyces culture Aerobic Bacterial culture, stain AFB culture & smear (Acid Fast, TB) AFB Blood culture Anaerobic culture Blood culture Body Fluid culture, stain
More informationSelected New Jersey Communicable Disease Regulations
COMMUNICABLE DISEASE OUTBREAK MANUAL New Jersey s Public Health Response Selected New Jersey Communicable Disease Regulations June 2013 Public Health Emergency Response Capability Requirements as stated
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Issue date: 17 August 2016 Worcestershire Royal Hospital Contact: Peter Jackson Microbiology Laboratory Tel: +44 (0) 1905 760192 Ext 30659
More informationPerform Gram stain only. Select Survey D5, Gram Stain
www.cap.org Bacteriology This flowchart is provided as a guide for ordering the appropriate Bacteriology Surveys for your laboratory s testing menu. In order to meet CLIA requirements for the subspecialty
More informationMinistry of Social Affairs and Health, Finland N.B. Unofficial translation. Legally valid only in Finnish and Swedish
Ministry of Social Affairs and Health, Finland N.B. Unofficial translation. Legally valid only in Finnish and Swedish No. 786/1986 Communicable Diseases Decree Issued on 31 October 1986 Section 1 was repealed
More informationSyndromic Testing: The right test, the first time.
Syndromic Testing: The right test, the first time. Don t guess. Know. Infectious disease diagnostics has evolved. BioFire s syndromic approach is making the world a healthier place. Many infectious diseases
More informationUSER GUIDE FOR SPECIMEN COLLECTION
DIAGNOSTIC LAB SPECIMEN COLLECTION GUIDE USER GUIDE FOR SPECIMEN COLLECTION ACM GLOBAL LABORATORIES DIAGNOSTIC SERVICES ACM Global Laboratories offers a comprehensive menu of services that support many
More information35.0. ug/ml Trough. 8.0 Carbamazepine 15.0 ug/ml Digoxin 2.5 ng/ml Gentamicin Peak Random Trough
The following are a list by department of Critical (Panic) Values: CHEMISTRY TEST LESS THAN GREATER THAN UNITS Glucose 0-7days 40 200 mg/dl Glucose > 7 days 50 400 mg/dl Glucose, CSF 25 mg/dl Sodium 121
More informationRev. 6/18/2018. Specimen Type. Usual Tests Ordered
Specimen Type ABSCESS Open Closed BITE WOUND BLOOD CULTURE BLOOD CULTURE, FUNGUS BLOOD CULTURE, AFB Usual Tests Ordered CXBLD Blood Culture CXFNB Culture, Fungal, Blood CAFBB Mycobacterial Culture, Blood
More informationIP Lab Webinar 8/23/2012
2 What Infection Preventionists need to know about the Laboratory Anne Maher, MS, M(ASCP), CIC Richard VanEnk PhD, CIC 1 Objectives Describe what the laboratory can do for you; common laboratory tests
More informationMOLECULAR DIAGNOSTIC PANELS and REAGENT KITS for NUCLEIC ACID EXTRACTION and RT-PCR of INFECTIOUS DISEASES and GENOMICS. Sep 2018
MOLECULAR DIAGNOSTIC PANELS and REAGENT KITS for NUCLEIC ACID EXTRACTION and RT-PCR of INFECTIOUS DISEASES and GENOMICS Sep 2018 STANDARDISED & EASY APPROACH TO PCR DIAGNOSTICS The diagnostic kits of MOLgen
More informationEQA PROGRAMME CATALOGUE. Version number CAT2019/01
2019 EQA PROGRAMME CATALOGUE www.qcmd.org Version number CAT2019/01 Contents An Introduction to the QCMD EQA Schemes 5 EQA Groups Blood Borne Virus 6 Central Nervous System 6 Congenital Infections 7 Drug
More informationSpecimen Type. Usual Tests Ordered
Specimen Type ABSCESS Open Closed BITE WOUND BLOOD CULTURE BLOOD CULTURE, FUNGUS BLOOD CULTURE, AFB Usual Tests Ordered CXBLD Blood Culture CXFNB Culture, Fungal, Blood CAFBB Mycobacterial Culture, Blood
More informationAbout the LIAISON MDX
The quality of treatment starts with diagnosis International Molecular Product MENU Simplexa Molecular Kits LIAISON MDX Molecular Reagents Primer Pairs About the LIAISON MDX The LIAISON MDX is an innovative
More informationPRODUCT# PRODUCT NAME VOLUME
The quality of treatment starts with diagnosis International Molecular Product MENU Simplexa Molecular Kits LIAISON MDX Molecular Reagents Primer Pairs About the LIAISON MDX The LIAISON MDX is an innovative
More informationMicrobiology- One Lab s Transformation from Ordinary to Extraordinary
Microbiology- One Lab s Transformation from Ordinary to Extraordinary Christy Saum, MT (ASCP) Laboratory Specialist-Microbiology Bryan Medical Center Bryan Medical Center-East In the Beginning In the Beginning
More informationCRITICAL VALUES, SIGNIFICANT FINDINGS LIST and STAT RESULTS
Page 1 of 6 Affected Sites: X Enterprise Chandler Good Samaritan CRITICAL VALUES: s are defined as an analytic result that suggests a clinical condition that may be lifethreatening and may require immediate
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Issue No: 001 Issue date: 08 June 2017 Dept of Microbiology Great Western Hospitals NHS Foundation Trust The Great Western Hospital Marlborough
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Department of Microbiology Princess Alexandra Hospital Hamstel Road Harlow CM20 1QX Contact: D Orriss Tel: +44 01279 827138 E-Mail: Debbie.Orriss@pah.nhs.uk
More informationWVU Medicine Specimens Submitted for Microbiology Testing
WVU Medicine Specimens Submitted for Microbiology Testing 1. Upper Respiratory a. anterior nares.. page 3 b. nasopharynx.. page 4 c. throat..... page 5 2. Lower Respiratory a. Sputum, tracheal aspirates,
More informationM. Khanna and S. Visuri
The ProPneumo1 Assay: Detection of Chlamydophila pneumoniae, Mycoplasma pneumoniae, and an Internal Control Using Multiplex PCR on Multiple Real Time PCR Systems S. Dollhopf,, W. Majewski,, P. Douglass,
More informationChain of Infection Agent Mode of transmission Contact (direct, indirect, droplet spread) Airborne Common-vehicle spread Host
Goals Microbiology of Healthcare-associated Infections William A. Rutala, Ph.D., M.P.H. Director, Statewide Program for Infection Control and Epidemiology and Research Professor of Medicine, University
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Luton and Dunstable University Hospital NHS Foundation Trust Lewsey Road Luton Bedfordshire LU4 0DZ Contact: Pauline Philip Tel: +44 (0)1582
More informationTest Menu. Infectious Diseases Laboratory. Division of Infectious Diseases Department of Medicine
Test Menu Infectious Diseases Laboratory Division of Infectious Diseases Department of Medicine July 2017 1 Atypical Pneumonia PCR Panel (APP)* The APP is an in-house developed, real-time (RT) PCR assay,
More informationAt the forefront of multiplex PCR
Prodesse At the forefront of multiplex PCR 2005 PRODUCT CATALOG Distributed by Standard Format Assays Real Time Format Assays Hexaplex Influenza A Influenza B Parainfluenza 1 Parainfluenza 2 Parainfluenza
More informationPathogen Matrix PATHOGEN CLAIM PPM (MG/L) / ORP TIME (MIN) DETAILS SOURCE
Pathogen Matrix PATHOGEN CLAIM PPM (MG/L) / ORP TIME (MIN) DETAILS SOURCE A. niger 90% At (0.166-0.246). 5 N/A http://aem.asm.org/cgi/ Acinetobacter baumannii Reached 25 20 ozone gas http://www.tersano.com/pdf/ajic_
More information12/12/2011. Atypical Pneumonia. Objectives. Causative Agents of Acute Pneumonia Bacteria. Causative Agents of Acute Pneumonia Other Agents
Objectives Atypical Pneumonia K. Sue Kehl, Ph.D., D(ABMM) Associate Professor, Pathology Medical College of Wisconsin Associate Director of Clinical Pathology & Technical Director of Microbiology, Children's
More informationShift from culture screening to molecular testing
Shift from culture screening to molecular testing Dr Panagiotis Pantelidis Lead Clinical Scientist / POCT and Clinical Trials Scientific Lead (Immunology / Virology) Pathology - Infection and Immunity
More informationVersion number CAT 2016/01
Version number CAT 2016/01 Contents An Introduction to the QCMD EQA Schemes 4 Disease Groups Blood Borne Virus 5 Central Nervous System 5 Congenital Infections 6 Drug Resistance 6 Exotic/Emerging Diseases
More informationAbout HPV. Human papillomavirus (HPV) is a group of viruses that are extremely common worldwide. Theree are more than 100 types of HPV, of which at
Cervical Cancer & HPV What is HPV Human papillomavirus (HPV) is a group of viruses that are extremely common worldwide. About HPV Theree are more than 100 types of HPV, of which at least 13 are cancer-causing
More informationLaboratory Evidence of Human Viral and Selected Non-viral Infections in Canada
Canada Communicable Disease Report ISSN 1188-4169 Date of publication: October 1998 Volume 24S7 Supplement Laboratory Evidence of Human Viral and Selected Non-viral Infections in Canada 1989 to 1996 Our
More informationInfection Control Manual Residential Care Part 3 Infection Control Standards IC6: Additional Precautions
IC6: 0110 Appendix I Selection Table Infection Control Manual esidential Care IC6: Additional Legend: outine Practice * reportable to Public Health C - Contact ** reportable by Lab D - Droplet A - Airborne
More informationAHS Laboratory Services Central Zone Guide to Lab Services Manual Cultures/Procedures
AEROBIC CULTURE Routine culture and routine aerobic culture are synonymous terms. Aerobic culture attempts to identify bacteria which grow in the presence of oxygen and are associated with potential infection
More informationMICROBIOLOGY CONTROL PRODUCTS
MICROBIOLOGY CONTROL PRODUCTS MOLECULAR QC MICROORGANISMS Table of Contents microbiologics.com 1 Customer Service 1 Technical Support 1 Worldwide Distribution 1 International Customers 2 USA Customers
More informationBACTERIOLOGY PROGRAMME AND PLAN OF TEACHING 3 rd Semester (academic year )
BACTERIOLOGY PROGRAMME AND PLAN OF TEACHING 3 rd Semester (academic year 2012-2013) 19. 10. 2012. Introduction in microbiology, bacterial taxonomy, general bacterial prop Bacterial structures, biosynthesis
More informationFifteen years of molecular EQA: progress and challenges
Fifteen years of molecular EQA: progress and challenges Anton M van Loon 1, Stuart West 2 and Paul Wallace 2 1 Department of Virology, UMC Utrecht, The Netherlands 2 QCMD, Glasgow, Scotland Key Issues
More informationIsolation Precautions in Clinics
Purpose Audience General principles Possible Exposures To define isolation precautions in a clinic setting. Clinics Isolation status should be determined primarily by the suspected disease and/or pathogen.
More informationPRODUCT RANGE
PRODUCT RANGE 2010-2011 MICROBIOLOGY - VIROLOGY Q - CMV Real Time Complete kit 96 RTK015 Detection of CMV DNA (MIEA) 4 Plasmidic controls at known titre for quantitative assays and CPE-DNA included in
More informationProtocols for Laboratory Verification of Performance of the FilmArray Respiratory Panel 2 plus (RP2plus)
Protocols for Laboratory Verification of Performance of the FilmArray Respiratory Panel 2 plus (RP2plus) Purpose The Clinical Laboratory Improvement Amendments (CLIA), passed in 1988, establishes quality
More informationM E D I C A L L A B O R A T O R Y
M E D I C A L L A B O R A T O R Y E V A L U A T I O N PARTICIPANT SUMMARY 2 0 1 7 Microbiology 2017 MLE-M1 Total Commitment to Education and Service Provided by ACP, Inc. Evaluation Criteria... 2 Microbiology
More informationrespirarna 2.0 real time RT-PCR Kit
Instruction for Use respirarna 2.0 real time RT-PCR Kit For the in-vitro detection of the RNA of Influenzavirus A, Influenzavirus B and Respiratory Syncytial Virus in clinical specimens. G01084-32 G01084-96
More informationSpecimen Type. Usual Tests Ordered
Specimen Type ABSCESS Open Closed BITE WOUND BLOOD CULTURE BLOOD CULTURE, FUNGUS BLOOD CULTURE, AFB Usual Tests Ordered CXBLD Blood Culture CXFNB Culture, Fungal, Blood CAFBB Mycobacterial Culture, Blood
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
Department of Microbiology St Helier Hospital Wrythe Lane Carshalton SM5 1AA Contact: Girish Patel Tel: +44 (0)20 82962468 E-Mail: girishkumar.patel@nhs.net Testing performed at the above address only
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Derriford Hospital Derriford Road Plymouth PL6 8DH Contact: John Hooper Tel: +44 (0) 1752 792369 E-Mail: johnhooper@nhs.net Website: www.plymouthhospitals.nhs.uk/pathologywelcome
More informationSchedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
Schedule of ccreditation United Kingdom ccreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK ccredited to Microbiology Ground Floor Link Building berdeen Royal Infirmary
More informationCal OSHA Title 8, Section 5199 Appendix D: Aerosol Transmissible Pathogens Laboratory
Cal OSHA Title 8, Section 5199 Appendix D: Aerosol Transmissible Pathogens Laboratory This appendix contains a list of agents that, when reasonably anticipated to be present, require a laboratory to comply
More informationS. Wesley Long, MD, PhD
Basic Molecular Microbiology: A Practical Case-Based Approach S. Wesley Long, MD, PhD Center for Molecular and Translational Human Infectious Diseases Research Houston Methodist Research Institute September
More informationMicrobiology Specimen Collection Instructions. Table I Culture and Sensitivities/TB
Specimen Page 1 of 10 Source / Type of Specimen Culture and Sensitivities () Abscess aspirate, wound aspirate in sterile screw cap or swab in charcoal. Obtain anaerobic swab from lab. Pilonidal and perirectal
More information