AP2 Lab 6 - Respiratory System, Lung Dissection, and Blood Groups/Types (Note: Lymphatic System is also fair game on next quiz)

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1 AP2 Lab 6 - Respiratory System, Lung Dissection, and Blood Groups/Types (Note: Lymphatic System is also fair game on next quiz) Use figs to identify the following on the human torso models. NASAL CAVITY CONCHAE (pronounced con-kay ) These are depicted best on the removable ½ head on the darker torso models. Find 3 thin, mucous membrane covered bony plates on the lateral walls of the nasal cavity. They increase the surface area exposed to incoming air, thus improving the warming, moistening, and cleansing of incoming air. PHARYNX (pronounced fair-inks ) This also is best depicted on the ½ head of the darker torsos models. It is the area at the back of the nasal cavity and oral cavity and before entering the trachea to lungs or esophagus to stomach. Anything in the pharynx has the possibility of going down either the esophagus to the stomach or the trachea to the lungs. The epiglottis determines which path is taken. (See epiglottis below.) In the lateral wall of the pharynx note the slit-like opening of the AUDITORY TUBE, (a.k.a. EUSTACHIAN TUBE - pronounced U station ) (a.k.a. PHARYNGOTYMPANIC TUBE) into the wall of the pharynx OYO: What is the function of the EUSTACHIAN TUBE? What is the purpose and what is the disadvantage? LARYNX (pronounced Lair-inks ) The larynx is a group of cartilages, ligaments, and muscles at the bottom of the pharynx and the top of the trachea. Lots of people simply call it the voice box or Adam s apple. While the larynx is depicted on each torso model it is best depicted on the separate larynx models. On the larynx models identify: THYROID CARTILAGE - the largest, shield shaped cartilage protruding anteriorly. It supports and protects the vocal cords. Palpate this on yourself and on another student. Feel it move as you swallow. CRICOID CARTILAGE a smaller wrap around cartilage just below the thyroid cartilage. With your finger find the narrow soft space between the thyroid and cricoid cartilages. OYO: What is a TRACHEOTOMY (A.k.a. cricothyrotomy)? EPIGLOTTIS If you re looking down into the larynx this will appear as a tongue-like flap. Separate the larynx model and view it from the medial view also. When the larynx is raised upward when swallowing this cartilage acts like a moveable flap that pushes down to cover the entrance to the larynx / trachea. It is supposed to divert food and water into the esophagus. Occasionally, solids or liquids will begin to enter the trachea by mistake causing an immediate and violent cough reflex to expel the object. OYO: Describe / explain the Heimlich Maneuver. VOCAL CORDS - visible as white folds of mucous membrane on the inner walls of the thyroid cartilages. Updated 8/22/2017 1

2 CONDUCTING ZONE PASSAGES - (usually pale blue on the models) THESE are the passageways for air to flow between the atmosphere and the alveoli during ventilation. TRACHEA the tubular passageway for air from just below the larynx to the lungs. C-shaped rings of cartilage found in the walls keep this airway open. The Cs open toward the posterior side facing the esophagus. OYO: Why have the open portion of the C face the esophagus? L & R PRIMARY BRONCHI (each is a BRONCHUS, a.k.a. MAIN STEM BRONCHUS ) These are the first two branches of the trachea. One leads to the L lung; the other to the R lung. The single, larger cartilage where they fork is called the CARINA. This is depicted best on the lighter colored torsos. The trachea and larger bronchi are lined with ciliated epithelial cells that constantly produce and move mucus upwards toward the pharynx where it is swallowed. OYOs: Why the mucus? Why the cilia? (fig. 22.7) How much mucus do you swallow per day? Eewwwwwww! One of the effects of cigarette smoke is to paralyze the movement of cilia. Relate this to the classic smoker s cough. Inhaled foreign objects usually end up lodged in the R main stem bronchus or the R lung and not the left. Why? This is depicted best on the lighter torsos. CONFIRM ACCURACY OF THESE FIRST 2 PAGES WITH YOUR INSTRUCTOR. Updated 8/22/2017 2

3 BRONCHIOLES (fig & 22.10) the very smallest subdivisions of the bronchi. They supply air to the microscopic alveolar air sacs. Their walls contain smooth muscle under involuntary control. Airway constriction/dilation is most significant here and can dramatically increase/decrease resistance to airflow. e.g. asthma, anaphylaxis ASTHMA Asthma is actually a form of allergic reaction involving the bronchioles. Many different allergens (pollens, cigarette smoke, stress, and exercise) may trigger an attack. What 3 physiologic changes cause breathing to become so difficult during an asthma attack? Emergency treatment for asthma often involves the medication ALBUTEROL and in severe cases EPINEPHRINE. Both are adrenergic (sympathomimetic) drugs. OYO: What will they cause the bronchioles to do? LUNGS (fig. 22.8) There are 3 lobes in the right lung and 2 in the left lung and various numbers of lobules in each lobe. The PRIMARY BRONCHI subdivide and the branches are called SECONDARY BRONCHI. The secondary bronchi subdivide to become TERTIARY BRONCHI, etc. to supply air to the many millions of ALVEOLI. The very smallest tubes are called BRONCHIOLES. They have incomplete rings of cartilage or none at all and are therefore capable of constriction/dilation called bronchoconstriction / bronchodilation. Lung tissue is very elastic. This elasticity combined with the surface tension inside alveoli contributes to lung recoil. ALVEOLI (fig & 22.10) are the only true RESPIRATORY ZONE STRUCTURES. These are the only structures where gas exchange occurs by diffusion across the walls. Are microscopic air sacs resembling clusters of grapes at the ends of the bronchioles. Are surrounded by blood capillaries Is where exchange of gasses between the air and blood takes place. If all of your alveoli were laid out flat they would cover both side walls the full length of the lab. Surface tension on the inside of each alveolus contributes to lung recoil. Explain why (how) oxygen moves into the blood and carbon dioxide moves out of the blood at this location. Which respiratory law (from lecture) explains the movement of gas molecules from a liquid to a gas or from a gas to a liquid? Updated 8/22/2017 3

4 PULMONARY ARTERIES (blue) and PULMONARY VEINS (red) (fig ) -- supply blood to and from the capillaries surrounding the alveoli. Explain why pulmonary arteries are blue and not red. PLEURAL MEMBRANES (figs & 22.13) Serous membranes surrounding the lungs. They secrete SEROUS FLUID (or PLEURAL FLUID) 1. for lubrication between the lung surfaces and thoracic wall. 2. to provide surface tension to hold the lungs against the thoracic wall Distinguish between VISCERAL PLEURA and PARIETAL PLEURA. PLEURAL CAVITY (figs & & 22.14) The extremely thin space between the visceral and parietal pleural membranes. The two membranes are actually in contact with each other but slide freely due to pleural fluid. The pressure here is slightly negative (-4 mmhg) because lung recoil causes the visceral pleural membrane to always try to pull away from the parietal pleural membrane of the thoracic wall. Distinguish between PNEUMOTHORAX and HEMOTHORAX. OYO: How is a pneumothorax treated/reversed? DIAPHRAGM (fig ) The dome shaped sheet of muscle that separates the thoracic cavity from the abdominal. It is the muscle primarily responsible for restful, quiet breathing. When it contracts it moves downward and somewhat flattens out thus increasing thoracic cavity size and causing inspiration. When it relaxes it raises upward into a dome shape thus decreasing thoracic cavity size and causing exhalation. Updated 8/22/2017 4

5 INTERNAL AND EXTERNAL INTERCOSTAL MUSCLES (fig ) Two layers of muscle between the ribs. The external intercostals can lift the ribs up & out for inhalation but are usually only involved in vigorous, extra deep inhalations. The internal intercostals can pull the ribs downward and inward for exhalation but are normally only involved when you need a forceful exhalation. During restful, quiet breathing exhalation is usually a passive process accomplished by. Define Pulmonary Ventilation: Explain why (how) air moves in and out of the lungs in response to contraction / relaxation of these respiratory muscles. Which respiratory law (from lecture) explains this process? CONFIRM ACCURACY WITH YOUR INSTRUCTOR Updated 8/22/2017 5

6 INSTRUCTIONS FOR LUNG DISSECTIONS **Note: Not all specimens will have all structures present. Identify the LARYNX, TRACHEA, ESOPHAGUS, LUNGS, and if present, the HEART and any remnants of the PERICARDIAL SAC. Use a metal probe to separate the esophagus from the trachea. The thoracic aorta may also be present. The walls of the aorta will be whiter and firmer and hold its shape better. Examine the trachea more closely and identify: LARYNX (not present on many specimens because they cut too low) Scrape away superficial tissues and muscle to expose and ID the THYROID CARTILAGE and CRICOID CARTILAGE. Note that the posterior wall of the trachea is soft and flexible allowing for easier passage of food. The wall flexes inward as a bolus of food passes down the esophagus. Find the soft space between the thyroid and cricoid cartilages. This is where a tube can be inserted during a TRACHEOTOMY (A.K.A. CRICOTHYROTOMY). Locate the EPIGLOTTIS and make it work the way it would when swallowing. What is its function? Look into the opening, called the GLOTTIS, and see the VOCAL CORDS. The cords are more like a pair of shelves than cords. Stick your finger or a probe into the esophagus where food would go. If you have one on your specimen, find the distal end of the esophagus where it connects to the stomach. What sphincter would be here? For what purpose? What disorder develops when this sphincter doesn t work well? TRACHEA Run your fingers up and down the anterior side of the trachea and feel the C-SHAPED RINGS OF CARTILAGE. What is their purpose? What kind of membrane lines the lumen of the trachea? For what purpose? BRONCHI and BRONCHIOLES Follow the trachea down until it splits and locate two or more PRIMARY BRONCHI. (These animal specimens may have more than two primary bronchi and the trachea may not split at a well-defined carina. Also their lungs don t necessarily have 3 lobes on the right and 2 on the left.) Feel the smooth outer surface of the lungs. What membrane is this? What do you call the fluid that lubricates it? Find any remnants of the PERICARDIAL SAC and heart and major blood vessels. Often you can see portions of the aorta, pulmonary trunk, and atria. Identify all that you can. **Invite your instructor over and confirm your identifications of all of the above. Updated 8/22/2017 6

7 1) INTUBATION: You ll need an AMBU BAG, ET TUBE, and SYRINGE. If the lungs are small, insert the endotracheal tube through the larynx into the trachea. [If the lungs are large then sever the trachea near the carina and insert the ET tube into a 1 o or 2 o bronchus.] Inflate the cuff with cc air from a syringe to seal the airway. There should be a snug fit and you should feel some resistance when you try to pull the tube out. Now connect the ambu bag to the ET tube and inflate the lungs. What are the microscopic air sacs being inflated.? Why do they tend to deflate immediately? (2 reasons) and 2) Using scissors or knife, insert one point into the trachea or a primary bronchus and split open the top side as you trace a portion of the bronchial tree as far out as you can go. Use paper towel to soak up any foam present so you can see the numerous branches of the bronchial tree. 3) Make a cross section midway through an intact lung to create a superior or inferior view. Identify bronchial tubes, arteries, and veins. 4) **Invite your instructor over and confirm your identifications of all of the above. Updated 8/22/2017 7

8 ABO BLOOD GROUPS (OYO practice quiz) [After taking notes and after typing your blood try answering these questions.] What determines whether a person has blood group A, B, AB or O? What is the magic question to ask when deciding which blood group can give to which blood type? Can group A safely give blood to group B? Why or why not? Can group A safely give blood to group AB? Why or why not? Can group AB safely give to group O? Why or why not? Can group O safely give to group A? Why or why not? Which blood group is the universal recipient? They can receive from anybody. But why? Which blood group is the universal donor? They can give to anybody. But why? RH BLOOD TYPES If the mother is type RH + and the fetus is type RH - is there a likelihood of erythroblastosis fetalis (HDN)? If the mother is type RH - and the fetus is type RH + is there a likelihood of erythroblastosis fetalis (HDN)? If the mother is type RH + and the fetus is type RH + is there a likelihood of erythroblastosis fetalis (HDN)? Updated 8/22/2017 8

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