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1 CURRICULUM VITAE NAME: AFFILIATION: CITIZENSHIP: HOME ADDRESS: OFFICE ADDRESS: Jordan T. Gladman Postdoctoral Research Associate The University of Virginia Charlottesville, Virginia Citizen of U.S.A Sugar Maple Court Charlottesville, VA Phone: (614) The University of Virginia 415 Lane Road Department of Pathology MR5 Building, Room 3332 Charlottesville, VA Phone: (434) EDUCATION: B.S. Molecular Biology Ohio Northern University, Ada, OH Ph.D. Integrated Biomedical Sciences The Ohio State University, Columbus, OH EXPERIENCE/SERVICE: Intern, Aerosol Science and Technology, Battelle Memorial Institute, Columbus, Ohio Graduate Research Associate, The Ohio State University, Columbus, Ohio. Ph.D. thesis in the laboratory of Dr. Dawn S. Chandler 2010-Present Postdoctoral Research Associate, The University of Virginia, Charlottesville, Virginia. Postdoctoral Studies in the laboratory of Dr. Mani Mahadevan HONORS AND AWARDS: 06/2007: Travel Award for the 11 th International Spinal Muscular Atrophy Research 09/2007: NRSA Fellowship from NIH: F31-GM A1, The Mouse SMN Gene: Potential Role in Modeling Spinal Muscular Atrophy 06/2008: Travel Award for the 12 th International Spinal Muscular Atrophy Research Jordan T. Gladman 1 Revised August, 2013

2 06/2009: Travel Award for the 13 th International Spinal Muscular Atrophy Research GRANTS AND CONTRACTS: NRSA Fellowship F31-GM A1 Gladman (PI) 09/30/ /29/2010 NIH/NIGMS The Mouse SMN Gene: Potential Role in Modeling Spinal Muscular Atrophy. Project: This project involves the comparison of splicing regulation of the mouse and human Survival Motor Neuron genes and testing of the efficacy of mouse SMA models involving the Smn gene itself. Total: 136, Role: Principal investigator (Mentored) PUBLICATIONS: a. Articles in Peer-Reviewed Journals 1. Gladman JT and Chandler DS. Intron 7 conserved sequence elements regulate the splicing of the SMN gene. Hum Genet. Volume 126, Issue 6 (2009), Page 833 DOI /s Bebee TW*, Gladman JT*, Chandler DS. Splicing regulation of the Survival Motor Neuron genes and implications for treatment of spinal muscular atrophy. Front Biosci, (2010 Jun 1);15: *Contributed equally to work 3. Gladman JT, Bebee TW, Edwards C, Wang X, Sahenk X, Rich MM, Chandler DS. A humanized Smn gene containing the SMN2 nucleotide alteration in exon 7 mimics SMN2 splicing and the SMA disease phenotype. Hum Mol Genet. Volume 19, Issue 21 (2010), Pages Bebee TW, Gladman JT, and Chandler DS. Generation of a Tamoxifen inducible SMN mouse for temporal SMN replacement. Genesis Pierson CR, Dulin-Smith AN, Durban AN, Marshall ML, Marshall JT, Snyder AD, Naiyer N, Gladman JT, Chandler DS, Lawlor MW, Buj-Bello A, Dowling JJ, Beggs AH. Modeling the human MTM1 p. R69C mutation in murine Mtm1 results in exon 4 skipping and a less severe myotubular myopathy phenotype. Hum Mol Genet. Volume 21, Issue 4 (2011), Pages Jacob AG, O'Brien D, Singh RK, Comiskey DF Jr, Littleton RM, Mohammad F, Gladman JT, Widmann MC, Jeyaraj SC, Bolinger C, Anderson JR, Barkauskas DA, Boris-Lawrie K, Chandler DS. Stress-induced isoforms of MDM2 and MDM4 correlate with high-grade disease and an altered splicing network in pediatric rhabdomyosarcoma. Neoplasia (Accepted 7/2013). 7. Gladman JT, Mandal M, Srinivasan V, Mahadevan MS. Age of Onset of RNA Toxicity Influences Phenotypic Severity: Evidence from an Inducible Mouse Model of Myotonic Dystrophy (DM1). PLOS One (Accepted 7/2013) b. Articles in Preparation: 1. Gladman JT*, Yadava R*, Yu Q, Mahadevan MS. NKX2-5, a Modifier and Marker of RNA Toxicity in Skeletal Muscle in Myotonic Dystrophy *Contributed equally to work Jordan T. Gladman 2 Revised August, 2013

3 2. Rehman S, Gladman JT, Periasamy A, Sun Y, Mahadevan MS. In vivo FRET based analysis to measure RNA-Protein foci interactions. c. Abstracts Regional, National, and International Meetings: 1. Gladman JT and Chandler DS. A New Model to Investigate the Differential Splicing of the Survival of Motor Neuron Gene in Spinal Muscular Atrophy. Rustbelt RNA Meeting, Mt. Sterling, Ohio, October Poster 2. Gladman JT and Chandler DS. SMN2 Silent Mutation Knock-In: A New Model for Spinal Muscular Atrophy. Pediatric Academic Societies Annual 2006 Meeting, San Francisco, CA, May Poster 3. Chandler DS, Gladman JT, and Bebee TW. Generation of a New Mouse Model to Help Determine the Therapeutic Time-Point for SMN Replacement Therapies. Families of Spinal Muscular Atrophy Meeting 2006, June Bebee TW, Gladman JT, and Chandler DS. Generation of a New Inducible Mouse Model to Help Determine the Therapeutic Time-Point for SMN Replacement Therapies. Rustbelt RNA Meeting, Mt. Sterling, Ohio, October Gladman JT and Chandler DS. SMN2 Exon 7 Knock-In: A New Spinal Muscular Atrophy Mouse Model. Rustbelt RNA Meeting, Mt. Sterling, Ohio, October 20 - October 21, Poster 6. Bebee TW, Gladman JT, and Chandler DS. Generation of a New Mouse Model to Help Determine the Therapeutic Time-Point for SMN Replacement Therapies: An Update. 11th Annual International Spinal Muscular Atrophy Research Meeting, Schaumburg, Illinois, June 21, Gladman JT, Bebee TW, and Chandler DS. SMN2 Mutation Knock-In: A New Model for Spinal Muscular Atrophy. 11th Annual International Spinal Muscular Atrophy Research Meeting, Schaumburg, Illinois, June 21, Poster 8. Bebee TW, Gladman JT, and Chandler DS. Generation of a new mouse model to help determine the therapeutic time-point for SMN replacement therapies: an update. Rustbelt RNA Meeting, Mt. Sterling, Ohio, October Gladman JT, Bebee TW, and Chandler DS. SMN 2 Exon 7 Knock-In: A New Spinal Muscular Atrophy Mouse Model. Rustbelt RNA Meeting, Mt. Sterling, Ohio, October Poster 10. Gladman JT, Bebee TW, and Chandler DS. Mouse Smn Exon 7 C>T Knock-in Mutation Mimics Humans SMN2 Splicing. 12th Annual International Spinal Muscular Atrophy Research Meeting, Boston, Massachusetts, June 19 - June 21, Podium Poster 11. Gladman JT, Bebee TW, and Chandler DS. Generation of an In Vitro System to Evaluate SMN2 Corrective Splicing Therapies. The Gordon Conference on The Biology of Post- Jordan T. Gladman 3 Revised August, 2013

4 Transcriptional Gene Regulation, June 30 - July 4, 2008, Colby College, Maine. Poster 12. Gladman JT and Chandler DS. Mouse Smn Exon 7 C>T: A Smn2 Like Knock-in Mouse Model of Spinal Muscular Atrophy. Rustbelt RNA Meeting, Mt. Sterling, Ohio, October 17 - October 18, Oral 13. Gladman JT, Bebee TW, and Chandler DS. Correct Splicing of the SMN Genes Requires Conserved Intronic Regions. Understanding Muscle: From Development to Disease, Columbus, Ohio, April 16, Poster 14. Bebee TW, Gladman JT, and Chandler DS. Determining the Therapeutic Time-Point for SMN Replacement in a Mouse Model of Spinal Muscular Atrophy. Understanding Muscle: From Development to Disease, Columbus, Ohio, April 16, Gladman JT, Bebee TW, Chandler DS. A Humanized Smn Gene Containing the SMN2 Nucleotide Alteration in Exon 7 Mimics the Spinal Muscular Atrophy (SMA) Disease Phenotype. The Fourteenth Annual Meeting of the RNA Society, Madison, WI, May 26 May 30, Gladman JT, Bebee TW, and Chandler DS. Direct Comparisons of the Mouse and Human SMN Gene Splicing. 13th Annual International Spinal Muscular Atrophy Research Meeting, Cincinnati, Ohio, June 18 - June 20, Gladman JT and Chandler DS. Splicing of the SMN Gene is Regulated by Conserved Sequence Elements Located in Intron 7. 13th Annual International Spinal Muscular Atrophy Research Meeting, Cincinnati, Ohio, June 18 - June 20, Poster 18. Gladman JT and Chandler DS. Splicing of the SMN Genes is Regulated by Conserved Sequence Elements Located in Intron Seven. Rustbelt RNA Meeting, Mt. Sterling, Ohio, October 16 - October 17, Oral 19. Gladman JT, Bebee TW, and Chandler DS. Elucidating the complex pre-mrna splicing regulation of the SMN genes using evolutionarily conserved sequence comparison. 15 th Annual Meeting of the RNA Society, Seattle, Washington, June 22 - June 26, Poster 20. Gladman JT, Yadava RS, Mandal M, Yu Q, Mahadevan MS Elucidating the Role of NKX2-5 Expression in Skeletal Muscle. International Myotonic Dystrophy Consortium 8, Clearwater Beach, Florida, November 30 December Selected Oral d. Institutional s 1. Smn2 Silent Mutation Knock-In: A New Model for Spinal Muscular Atrophy. Molecular Biology and Cancer Genetics Program Biennial Retreat, Dellroy, Ohio November Using the Mouse Survival Motor Neuron Gene to Better Understand Spinal Muscular Atrophy. The Ohio State University s Motor Neuron, Columbus Ohio January The SMN Gene in Spinal Muscular Atrophy. The Ohio State University s Muscle, Columbus, Ohio February Jordan T. Gladman 4 Revised August, 2013

5 4. Intron 7 Conserved Sequence Elements Regulate the Splicing of the SMN Gene. The Ohio State University s Motor Neuron, Columbus, Ohio May Understanding RNA Splicing to Fight Spinal Muscular Atrophy and other RNA Splicing Diseases. The Research Institute at Nationwide Children's Hospital Cancer, Columbus Ohio, December Elucidating the Role of NKX2-5 Expression in Skeletal Muscle The University of Virginia Pathology Retreat, Charlottesville, VA February Award Winning Oral 7. Elucidating the Role of NKX2-5 Expression in Skeletal Muscle The University of Virginia Pathology Retreat, Charlottesville, VA February Award Winning Poster REVELENT SKILLS: Capable of designing and implementing multiple experiments Experienced and proficient with standard molecular biology and cell culture techniques, RNA biology techniques and using mouse as a model system for disease Trained several rotation students and lab members with various techniques and critical thinking related to different projects in our lab Lab Radiation Safety Officer and Lab Ordering/Supply Manager Presented at numerous national, regional, and local conferences Experienced and proficient using multiple word processing, data analysis, statistical, and graphic computer programs on both Windows and Macintosh platforms Jordan T. Gladman 5 Revised August, 2013

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