Siriraj Dust Mite Center for Services and Research (SDMC) (Progress on the Activities) Asst.Prof. Dr. Nat Malainual
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1 Siriraj Dust Mite Center for Services and Research (SDMC) (Progress on the Activities) Asst.Prof. Dr. Nat Malainual Faculty of Medicine Siriraj Hospital Mahidol University, Thailand
2 Siriraj Dust Mite Center Organization profile Establishment of large-scale dust mite cultures Services; mite selling, testing services Research Collaboration Social activities Future activities
3 Mahidol University Faculty of Medicine Siriraj Hospital Department of Parasitology House Dust Mite and Allergic Disease Research Project in June, 2001 Siriraj Dust Mite Center for Services and Research since January, 2007
4 SDMC Concept Dust Mite Cultures Publications Innovations Services & Commercialization
5 Vision To be the premier BioResource Center for dust mites in Asia.
6 Motto When you think of dust mites, Think of SDMC, Thailand
7 Missions 1. Maintain the cultivation of dust mite cultures 2. Provide the dust mites to researchers / industries 3. Develop the testing services for anti-mite products regarding consumer protection 4. Conduct the research study focusing on dust mites 5. Make the collaboration with academic and private sectors to share knowledge on dust mites aspects
8 Establishment of Dust mite cultures
9 The way from laboratory cultures to BRC
10 SDMC Present
11 4 weeks Using mite isolator Purity 99% Harvestation
12 Allergon, Sweden SDMC, Thailand Microscopic Physical Appearance CSL, UK Greer Lab, USA
13 Uses of Dust Mites Dust Mite extracts: for diagnostic / therapeutic purposes Biological study: climate control, growth rate, Taxonomic study: genetic identification,. Testing anti-mite products: mite proof, herbal acaricides etc.
14 Quality Assurance of Dust Mite Product Pre-culture Culturing Post-culture Mite strains Food/Media Containers Planning Mite strains Contaminations Growth rates Environment Correct species Purity Waste management Stock management
15 Good Dust mite products Single species 99% purity High yield Dermatophagoides pteronyssinus Dermatophagoides farinae Blomia tropicalis
16 Testing Services
17 Anti-mite products Acaricides Mite-proof covers Anti-mite covers Air purifier/air sterilizer Anti-mite mattress
18 Anti-mite product testing Mite-proof fabrics testing Anti-mite agents testing (Chemical & Natural products) Siriraj chamber Blocking allergen Killing mites Regular bed sheet Physical Efficacy on mite eradication Mite penetration Heat escape method Siriraj chamber method Blocking allergen ELISA Dust leakage Air permeability Thread count % killed mites
19 Dust Mite Allergen Assessment Mite allergen Mite count Mite identification
20 Support the dust mite product and testing services for Thailand and other countries; Singapore, China, Taiwan, Philippines, Australia, India, etc.
21 Quality Improvement On-going process to improve the quality system to be accredited against ISO 9001: 2008 requirements. Tentative Date of Accreditaion => February 2014
22 Research
23 Asian Pac J Allergy Immunol 2012;30: To detect multiple target genes by using multiple primers Simultaneously identitied 3 mite species in one reaction High specificity and high sensitivity
24 Specificities Lane 1 : 0.1 ng DP + 10 ng DF (1:100) Lane 2 : 0.1 ng DF + 10 ng DP (1:100) Lane 3 : 0.1 ng BT + 10 ng DP (1:100) Lane 9 : 0.1 ng each of DP, DF, BT Lane 10 : 10 ng each of DP, DF, BT Lane 5 : 10 ng DP ng DF (100:1) Lane 6 : 10 ng DF ng DP (100:1) Lane 7 : 10 ng BT ng DP (100:1)
25 Sensitivities 1 mite => 10 ng gdna
26 House Dust Sample DNA extraction Multiplex-PCR Results On-going : Quantitative PCR
27 On-going Research Quantitative PCR for mite identification Dust mite allergenomes Enriched medium for dust mite production Climate controller for dust mite control
28 Collaborations Siriraj - The Thailand Textile Institute (THTI) for developing the anti-mite textiles Siriraj - Greater Pharma Manufacturing Co.,LTD for industrial scale of mite allergen vaccine production
29 Regional Educational Hub International HDM Workshop Workshop : All you need to know about House Dust Mites, 9-12 February 2010 at SDMC Mahidol University, Bangkok, Thailand Workshop : Significant Impact of Allergic Diseases & House Dust Mite Allergy 4-6 April 2010 at Dhulikhel Hospital, Kathmandu University, Nepal
30 Local & International Visitors
31
32 Up-coming Events International Workshop on House Dust Mites 9 November 2013 in Manila, Philippines June 2014 in Bangkok, Thailand (AEC participants) Local Workshop on House Dust Mites February 2014 in Bangkok, Thailand
33 Siriraj Dust Mite Center for Services and Research (SDMC) Service Research Academic Production Testing Training Social activities Publications Innovation
34 Future Activities Contributing dust mite specimen as research / teaching materials Driving the criteria for anti-mite products Establishing the certification service for anti-mite products (for consumer protection)
35 Thank you very much for your attention House Dust Mite Network Think of House Dust Mites Think of SDMC-Thailand
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