A712(19)- Test slide, Breast cancer tissues with corresponding normal tissues
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1 A712(19)- Test slide, Breast cancer tissues with corresponding normal tissues (formalin fixed) For research use only Specifications: No. of cases: 12 Tissue type: Breast cancer tissues with corresponding normal tissues No. of spots: 1 spot from each cancer case (12 spots) 12 non-neoplastic spots (12 spots) Total spots: 24 Corresponding normal tissues with cancers: Yes Diameter: 1. 0 mm Documents: Product specification: layout, summary of tissue spots H&E stained images Detailed pathological information Layout: P T
2 A712(19)- Test slide, Breast cancer tissues with corresponding normal tissues Summary of tissue spots (formalin fixed) For research use only P T
3 A712(19)- Test slide, Breast cancer tissues with corresponding normal tissues (formalin fixed) For research use only QC sheet_lot# Hematoxylin and Eosin staining A1 A2 A3 A4 A5 B1 B2 B3 B4 B5 C1 C2 C3 C4 C5 D1 D2 D3 D4 D5 E1 E2 F1 F2 P T
4 1 A 1 f 62 Breast, right, partial mastectomy: 1. Size: 1.8x1.2cm 2. Black's nuclear grade: II (moderately differentiated) 3. Modified Bloom-Richardson's histological grade: III (tubule formation: III, nuclear pleomorphism: II, mitosis: III) 4. Intraductal component: less than 5% 5. Resection margins: Negtive 6. Vascular invasion: Absent 7. Perineural invasion: Absent 8. Microcalcification: Absent 9. Immunohistochemistry: ER(-), PR(-), C erb B2(+:3+/3+) Lymph node, sentinel(0/1): Free of tumor. 2 B 1 f 62 non-neoplastic non-neoplastic from A1 3 A 2 f 79 Breast, right, modified radical mastectomy: 1. Size: 2x1.8x1.5cm 2. Site: RIL quadrant, 4cm away from the nipple 3. Black's nuclear grade: II (moderately differentiated) 4. Modified Bloom-Richardson's histological grade: II (tubule formation: III, nuclear pleomorphism: III, mitosis: I) 5. Intraductal component: 5% 6. Resection margins: Negative 7. Vascular invasion: Absent 8. Immunohistochemistry: ER(+), PR(+), C erb B2(-) Nipple and areola: Free from tumor extension. Overlying skin: Extension of the tumor. Underlying fascia: Free from tumor extension. Lymph node, axillary(0/17): Free from tumor extension. 4 B 2 f 79 non-neoplastic non-neoplastic from A2
5 5 A 3 f 54 Breast, left, modified radical mastectomy: 1. Size: 1.3x1.0cm 2. Black's nuclear grade: II (moderately differentiated) 3. Modified Bloom-Richardson's histological grade: I (tubule formation: I, nuclear pleomorphism: II, mitosis: I) 4. Intraductal component: < 5% 5. Resection margins: Negative 6. Vascular invasion: Absent 7. Perineural invasion: Absent 8. Microcalcification: Present in invasive cancer, in ductal in situ, in associated with benign epithelium, in stroma immunohistochemistry: ER(+), PR(+), C erb B2(-:0/3+) 10. Ductal in situ in the background 1) Nuclear grade: Low 2) Histological grade: Low 3) Necrosis: Absent 4) Architectural types: Micropapillary 5) Extent of DCIS: Approximately 4x3.5cm 6) Microinvasion: Absent 11. Associated benign change: Adenosis and papillomatosis. Underlying fascia: Free of tumor. Lymph node, sentinel(0/3): Free of tumor. Breast, right, mastectomy: Main mass: Infiltrating ductal, nuclear grade II,Histologic grade II with minimal intraductal component (< 5%). Surrounding parenchyma: 1. Ductal in situ, low grade. 2. Papillomatosis. 3. Adenosis. Underlying fascia; free of tumor Result of immunohistochemistry.:er, PR; positive / C erb B2; negative 6 B 3 f 54 non-neoplastic non-neoplastic from A3
6 7 A 4 f 54 Breast, left, partial mastectomy: 1. Size: 2.2x1.5x1.0cm 2. Black's nuclear grade: II (moderately differentiated) 3. Modified Bloom-Richardson's histological grade: III (tubule formation: III, nuclear pleomorphism: II, mitosis: III) 4. Intraductal component: 15% 5. Resection margins: negative 6. Vascular invasion: Present 7. Perineural invasion: Absent 8. Microcalcification: Absent 9. Immunohistochemistry: ER(+), PR(+), C erb B2(-:1/3 +) Lymph node, sentinel(0/1): Free of tumor. 8 B 4 f 54 non-neoplastic non-neoplastic from A4
7 9 A 5 f 61 Breast, left, modified radical mastectomy: 1. Size: 4.3x2.2cm 2. Site: LUO quadrant, 2 o'clock area, 5cm away from the nipple 3. Black's nuclear grade: I (poorly differentiated) 4. Modified Bloom-Richardson's histological grade: III (tubule formation: III, nuclear pleomorphism: III, mitosis: III) 5. EIC: present 6. Vascular invasion: Absent 7. Perineural invasion: Absent 8 Microcalcification: Absent 9.Immunohistochemistry: ER(-), PR(+), C erb B2(-:1+/3+) 10. Associated benign changes: Ductal in situ in the background 1) Nuclear grade: High 2) Histological grade: High 3) Necrosis: Present (punctate) 4) Architectural types: Solid, Micropapillary 5) Extent of DCIS: 7.3x6cm 6) Calcification: Absent 7) Microinvasion: Present, multifocal Nipple and areola: Free of tumor. Overlying skin: Free of tumor. Underlying fascia: Free of tumor. Lymph node, axillary(3/12): Tumor metastasis in 3 out of 12 lymph nodes without pericapsular tumor spread (the largest tumor deposit: 1.1x0.8cm) 10 B 5 f 61 non-neoplastic non-neoplastic from A5
8 11 C 1 f D 1 f 69 non-neoplastic non-neoplastic from C1 13 C 2 f 45 Phyllodes tumor 14 D 2 f 45 non-neoplastic non-neoplastic from C2 15 C 3 f D 3 f 58 non-neoplastic non-neoplastic from C3 Breast, right, partial mastectomy: 1. Size: 2.2x1.3cm 2. Black's nuclear grade: I (poorly differentiated) 3. Modified Bloom-Richardson's histological grade:iii (tubule formation: III, nuclear pleomorphism: III, mitosis: III) 4. Intraductal component: 10% 5. Resection margins: Negative 6. Vascular invasion: Absent 7. Perineural invasion: Absent 8. Microcalcification: Present in invasive cancer, in ductal in situ 9. ER(+), PR(+), C erb B2(weak+:2+/3+) Underlying fascia: Free of tumor. Lymph node, sentinel(0/2): Free of tumor. Breast, site unstated, excision: 1. Phyllodes tumor, borderline(low grade malignant), with florid ductal epithelial hyperplasia. 2. The sections show moderately cellular stroma with characteristic leaf-like pattern. 3. Focally noted is increased mitoticactivity (2 to 3 per 10 hpf) and moderate cellular atypia. Breast, left, excision: Infiltrating ductal with predominant cribriform pattern 1. size: 2.5x2.5x1.5cm. 2. Black's nuclear grade II (moderately differentiated). 3. modified Bloom-Richardson's histological grade II (tubule formation:i, nuclear pleomorphism:ii, mitosis:iii). 4. no lymphovascular permeation. 5. non-neoplastic breast: Fibrocystic change with apocrine metaplasia. 6.Result of immunohistochemical stainingnote: ER(+), PR(+), C erb B2(-).
9 17 C 4 f D 4 f 66 non-neoplastic non-neoplastic from C4 19 C 5 f 54 infiltrating ductal 20 D 5 f 54 non-neoplastic non-neoplastic from C5 Breast, right, partial mastectomy: 1.Infiltrating ductal, with 1) Bloom and Richardson's histologic grade: II (tubule formation:iii, nuclear grade:ii, mitotic index:ii). 2) Black's nuclear grade: II. 3) no definitive lymphatic invasion. 4) intraductal componenht: 10%. 2.Mammary duct ectasia with calcification. 3.Fibroadenomatoid hyperplasia with calcification. 4.Result of immunohistochemical staining: ER(-), PR(-), C erb B2(+). 5.Result of flow cytometry: Diploid DNA pattern. Breast, left, modified radical mastectomy: Infiltrating ductal 1) modified Bloom 2) Richardson's histological grade: III (tubule formation: II, nuclear pleomorphism: III, mitotic activity: III) 3) Black's nuclear grade: I 4) occasional vascular invasion 5) no intraductal component 6) size of the tumor: 6x4.5x2.3cm 7) surrounding parenchyme: papillomatosis 2.Nipple and areola: Free of tumor. 3.Overlying skin: Free of tumor. 4.Underlying fascia: Free of tumor. 5. Lymph nodes, axillary(2/17): Metastasis in 2 out of 17 nodes. 6. Result of immunohistochemical stain: ER(-), PR(+), C erb B2(2+/3)
10 21 E 1 f 70 infiltrating ductal Breast, left, modified radical mastectomy: infiltrating ductal with 1) modified Bloom and Richardson's histologic grade: II (tubule formation: II, nuclear pleomorphism: II, mitotic index: III) 2) Black's nuclear grade: II 3) intraductal component: 1% 4) no lymphovascular invasion 5) size: 2.5x2x1.4cm and 0.7x0.7x0.5cm 6) nipple and areola: free of tumor 7) overlying skin and underlying fascia: free of tumor 8) lymph nodes, axilla(0/8): free of tumor metastasis in all 8 nodes 9) result of immunohistochemical staining: ER(+), PR(+), C erb B2(+,-:2+). 22 F 1 f 70 non-neoplastic non-neoplastic from E1 23 E 2 f 38 infiltrating ductal 24 F 2 f 38 non-neoplastic non-neoplastic from F1 Breast, left, modified radical mastectomy: 1.Infiltrating ductal 1) size: 6x4.5cm 2) histologic grade I (tubular formation II, pleomorphism II, mitosis I) 3) nuclear grade II 4) intravasclar permeation: uncertain 5) intraductal component, about 30% 2.Nipple and areola: Free of tumor. 3.Overlying skin and underlying fascia: Free of tumor. 4.Regional lymph nodes: Free of tumor in all nodes (0/18).
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