Title. CitationCancer science, 109(4): Issue Date Doc URL. Rights(URL)
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1 Title Toll-like receptor 3 signal augments radiation-induc Yoshida, Sumito; Shime, Hiroaki; Takeda, Yohei; Nam, Author(s) Hiroki; Kasahara, Masanori; Seya, Tsukasa CitationCancer science, 19(): Issue Date 18- Doc URL Rights(URL) Type article Additional There Information are other files related to this item in HUSCAP File Information cas1353-sup-1-supinfo.pdf (Supporting Informatio Instructions for use Hokkaido University Collection of Scholarly and Aca
2 Table S1. Antiboides used in this study. Antibody (and related reagent) Clone Supplier FITC-anti-mouse CD3ε 15-C11 Biolegend PE/Cy7-anti-mouse CD3 17A AlexaFluor7-anti-mouse CD8α APC-anti-mouse CD8α PE/Cy7-anti-mouse/human CD11b M1/7 APC-anti-mouse CD11c N18 PE/Cy7-anti-mouse CD11c APC-anti-mouse/human CD IM7 AlexaFluor7-anti-mouse CD5. 1 APC/Cy7-anti-mouse CD5. 3-F11 FITC-anti-mouse CD6L MEL-1 APC-anti-mouse F/8 BM8 FITC-anti-mouse F/8 APC-anti-mouse Ly6G/Ly6C (Gr-1) RB6-8C5 PE-anti-mouse TNF-α MP6-XT Purified anti-mouse CD16/3 *used in Fc blocking 93 PE-Rat IgG1, κ *Isotype control of PE-anti-mouse TNF- α BMa ebioscience T-select H-Kb OVA Tetramer-SIINFEKL-PE MBL ViaProbe *used for excluding dead cells BD Bioscience Table S. Primers for RT-qPCR used in this study. Forward Reverse Ccl3 5'-TTGAAACCAGCAGCCTTTGC-3' 5'-CTTTGGAGTCAGCGCAGATCT-3' Ccl 5'-GCCCTCTCTCTCCTCTTGCT-3' 5'-GGAGGGTCAGAGCCCATT-3' Ccl5 5'-TGCCCACGTCAAGGAGTATTT-3' 5'-TCGAGTGACAAACACGACTGC-3' Cxcl9 5 -GATAAGGAATGCACGATGCTC-3 5 -TCTCCGTTCTTCAGTGTAGCAA-3 Cxcl1 5 -GTGTTGAGATCATTGCCACGA-3 5 -GCGTGGCTTCACTCCAGTTAA-3 Cxcl11 5 -GGCTGCGACAAAGTTGAAGTGA-3 5 -TCCTGGCACAGAGTTCTTATTGGAG-3 Fasl 5'-TTTCAGAGGGTGTACTGGGG-3' 5'-TGGTTGGAATGGGATTAGGA-3' Gapdh 5 -GCCTGGAGAAACCTGCCA-3 5 -CCCTCAGATGCCTGCTTCA-3 Gzmb 5 -GCCTGGAGAAACCTGCCA-3 5 -CCCTCAGATGCCTGCTTCA-3 Ifnb 5'-CCAGCTCCAAGAAAGGACGA-3' 5'-CGCCCTGTAGGTGAGGTTGAT-3' Ifng 5 -GATATCTGGAGGAACTGGCAAAAG-3 5 -AGAGATAATCTGGCTCTGCAGGAT-3 Prf1 5 -CAAGGTAGCCAATTTTGCAGC-3 5 -GGCGAAAACTGTACATGCGAC-3 Tbx1 5 -CAACCAGCACCAGACAGAGA-3 5 -CCACATCCACAAACATCCTG-3
3 PolyI:C PolyI:C LLC-OVA-implanted WT B6 mice (s.c.) (Around. cm 3 ) PolyI:C PolyI:C h Day Day 1 (i.p., 1 µg/head) (X-ray, 15 Gy, tumor-local) Measurement of tumor volume Tumor volume (cm 3 ) WT B6 Non- PolyI:C 1.5 PolyI:C Days after start of treatment Figure S1. Pre-treatment of polyi:c with was more efficient than post-treatment in tumor regression. LLC-OVA-implanted WT B6 mice were intraperitoneally injected with polyi:c (1 μg/head) or performed 15 Gy of ionizing radiation () when tumor volume reached around. cm 3. After h, polyi:c administration or was performed following the time table in this figure. n = 3- mice per group. Data are shown as average (SD) tumor size.
4 Tumor volume (cm3) A Experiment #1 Non- Non- + anti-cd8β Ab + anti-cd8β Ab Non- Non- + anti-cd8β Ab + anti-cd8β Ab 3 B Experiment # Non- 15 Gy TUNEL n.s Days after start of treatment Figure S. damaged tumor cells and suppressed tumor growth independent of CTLs. (A) Growth of LLC-OVA tumor on WT B6 mice with or without anti-cd8β antibody (Ab) treatment. Anti-CD8β Ab was injected into tumor-bearing mice -8 h before 15 Gy of ionizing radiation (). Additional Ab injection was performed on 5-6 days after irradiation. Similar two experiments were performed. Data are shown as average (SD) tumor size. n.s., not significant. In experiment #1, n = 3 mice per group. In experiment #, n = 3-5 mice per group. (B) LLC-OVA-implanted mice were locally irradiated with 15 Gy of X-ray on tumor site. After 8 h, tumors were harvested, fixed with 1% neutralized buffered formalin at C for 3 h. Then, samples were equilibrated with 15% and 3% sucrose/pbs, mounted in O.C.T. compound (Sakura Finetek Japan), and flozen. Blocks were sliced and stained with the In Situ Cell Death Detection Kit, Fluorescein (Roche). Specimens were analyzed using BZ-9 (KEYENCE). Scale bars, 5 μm. The pictures are representative of two independent experiments.
5 PolyI:C PolyI:C LLC-OVA-implanted WT B6 mice (s.c.) (Around. cm 3 ) PolyI:C h Day Day 1 PolyI:C (i.p., 1 µg/head) (X-ray, 15 Gy, tumor-local) FACS (Day8) Gated on CD5 + cells in tumor Non- PolyI:C CD11b PolyI:C PolyI:C Gr-1 Figure S3. Frequency of intratumor CD11b + Gr-1 + cells was different in pre- or post- polyi:c injection with radiation. LLC-OVA-implanted WT B6 mice were treated with polyi:c and following the time table in this figure. Tumors were harvested on day 8 after the start of treatment and FACS analysis was performed. Plots are representative results of two with similar outcomes.
6 LLC-OVA (in vitro) TNF-α h h WST-1 Check cell viavility 1 Cell viability (%) PBS TNF-α.1 ng/ml TNF-α 1 ng/ml TNF-α 1 ng/ml Radiation dose (Gy) Figure S. TNF-α post-treatment decreased LLC-OVA cell viability combinatry with. LLC-OVA cells were subjected to 15 Gy of and h later, TNF-α was treated. After h culture, cell viability was assessed by WST-1 assay. n = 3. Data represent the means (SD).
SUPPLEMENTARY METHODS
SUPPLEMENTARY METHODS Histological analysis. Colonic tissues were collected from 5 parts of the middle colon on day 7 after the start of DSS treatment, and then were cut into segments, fixed with 4% paraformaldehyde,
Supplementary Figure S1. Flow cytometric analysis of the expression of Thy1 in NH cells. Flow cytometric analysis of the expression of T1/ST2 and
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IL-6Rα IL-6RαT-KO KO. IL-6Rα f/f bp. f/f 628 bp deleted 368 bp. 500 bp
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Supplementary Figure 1 Cytokine pattern in skin in response to urushiol. Wild-type (WT) and CD1a-tg mice (n = 3 per group) were sensitized and challenged with urushiol (uru) or vehicle (veh). Quantitative
Supplementary Materials for
immunology.sciencemag.org/cgi/content/full/2/16/eaan6049/dc1 Supplementary Materials for Enzymatic synthesis of core 2 O-glycans governs the tissue-trafficking potential of memory CD8 + T cells Jossef
Blocking antibodies and peptides. Rat anti-mouse PD-1 (29F.1A12, rat IgG2a, k), PD-
Supplementary Methods Blocking antibodies and peptides. Rat anti-mouse PD-1 (29F.1A12, rat IgG2a, k), PD- L1 (10F.9G2, rat IgG2b, k), and PD-L2 (3.2, mouse IgG1) have been described (24). Anti-CTLA-4 (clone
Supplementary Figure 1
Supplementary Figure 1 a Percent of body weight! (%) 4! 3! 1! Epididymal fat Subcutaneous fat Liver SD Percent of body weight! (%) ** 3! 1! SD Percent of body weight! (%) 6! 4! SD ** b Blood glucose (mg/dl)!
Title of file for HTML: Supplementary Information Description: Supplementary Figures and Supplementary Table
Title of file for HTML: Supplementary Information Description: Supplementary Figures and Supplementary Table Title of file for HTML: Peer Review File Description: Innate Scavenger Receptor-A regulates
L-selectin Is Essential for Delivery of Activated CD8 + T Cells to Virus-Infected Organs for Protective Immunity
Cell Reports Supplemental Information L-selectin Is Essential for Delivery of Activated CD8 + T Cells to Virus-Infected Organs for Protective Immunity Rebar N. Mohammed, H. Angharad Watson, Miriam Vigar,
Fisher et al. Supplemental Figure 1
Supplemental Figure 1 A TNF IL-1 IL-6 CCL2 CCL5 CXCL10 pg/mg total protein 50 30 10 4,000 3,000 2,000 1,000 n.d. 1 1 14,000 12,000 10,000 8,000 6,000 4,000 2,000 6,000,000 CT26 5,000 16,000 B16 4,000 12,000
Supplementary Figure 1. Characterization of basophils after reconstitution of SCID mice
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SUPPLEMENTARY FIGURES
SUPPLEMENTARY FIGURES Supplementary Figure 1: Chemokine receptor expression profiles of CCR6 + and CCR6 - CD4 + IL-17A +/ex and Treg cells. Quantitative PCR analysis of chemokine receptor transcript abundance
Supplementary Figure 1: TSLP receptor skin expression in dcssc. A: Healthy control (HC) skin with TSLP receptor expression in brown (10x
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sequences of a styx mutant reveals a T to A transversion in the donor splice site of intron 5
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CD44
MR1-5-OP-RU CD24 CD24 CD44 MAIT cells 2.78 11.2 WT RORγt- GFP reporter 1 5 1 4 1 3 2.28 1 5 1 4 1 3 4.8 1.6 8.1 1 5 1 4 1 3 1 5 1 4 1 3 3.7 3.21 8.5 61.7 1 2 1 3 1 4 1 5 TCRβ 2 1 1 3 1 4 1 5 CD44 1 2 GFP
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1 2 3 4 5 6 7 8 9 10 11 Supplementary Figure 1. H-PGDS deficiency does not affect GI tract functions and anaphylactic reaction. (a) Representative pictures of H&E-stained jejunum sections ( 200 magnification;
well for 2 h at rt. Each dot represents an individual mouse and bar is the mean ±
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Supporting Information
Supporting Information Desnues et al. 10.1073/pnas.1314121111 SI Materials and Methods Mice. Toll-like receptor (TLR)8 / and TLR9 / mice were generated as described previously (1, 2). TLR9 / mice were
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Supplemental figure 1. PDGFRα is expressed dominantly by stromal cells surrounding mammary ducts and alveoli. A) IHC staining of PDGFRα in
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doi: 1.138/nature775 4 O.D. (595-655) 3 1 -ζ no antibody isotype ctrl Plated Soluble 1F6 397 7H11 Supplementary Figure 1 Soluble and plated anti- Abs induce -! signalling. B3Z cells stably expressing!
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doi:10.1038/nature10134 Supplementary Figure 1. Anti-inflammatory activity of sfc. a, Autoantibody immune complexes crosslink activating Fc receptors, promoting activation of macrophages, and WWW.NATURE.COM/NATURE
MATERIALS AND METHODS. Neutralizing antibodies specific to mouse Dll1, Dll4, J1 and J2 were prepared as described. 1,2 All
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SUPPORTING INFORMATIONS
SUPPORTING INFORMATIONS Mice MT/ret RetCD3ε KO α-cd25 treated MT/ret Age 1 month 3 mnths 6 months 1 month 3 months 6 months 1 month 3 months 6 months 2/87 Survival 87/87 incidence of 17/87 1 ary tumor
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Supporting Information
Supporting Information Idoyaga et al. 10.1073/pnas.0812247106 SSC a) Single cell suspension 99 Aqua b) Live cells 96 -W c) Singlets 92 -A CD19+ER119 d) CD19 ER119 cells 97 CD3 e) CD3 cells 27 f) DX5 cells
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Supplemental Figure 1
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a surface permeabilized
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for six pairs of mice. (b) Representative FACS analysis of absolute number of T cells (CD4 + and
SUPPLEMENTARY DATA Supplementary Figure 1: Peripheral lymphoid organs of SMAR1 -/- mice have an effector memory phenotype. (a) Lymphocytes collected from MLNs and Peyer s patches (PPs) of WT and SMAR1
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Gr-1 Gr-1 Gr-1 Bezzi et al., Supplementary Figure 1 a Gr1-CD11b 3 months Spleen T cells 3 months Spleen B cells 3 months Spleen Macrophages 3 months Spleen 15 4 8 6 c CD11b+/Gr1+ cells [%] 1 5 b T cells
activation with anti-cd3/cd28 beads and 3d following transduction. Supplemental Figure 2 shows
Supplemental Data Supplemental Figure 1 compares CXCR4 expression in untreated CD8 + T cells, following activation with anti-cd3/cd28 beads and 3d following transduction. Supplemental Figure 2 shows the
D CD8 T cell number (x10 6 )
IFNγ Supplemental Figure 1. CD T cell number (x1 6 ) 18 15 1 9 6 3 CD CD T cells CD6L C CD5 CD T cells CD6L D CD8 T cell number (x1 6 ) 1 8 6 E CD CD8 T cells CD6L F Log(1)CFU/g Feces 1 8 6 p
pplementary Figur Supplementary Figure 1. a.
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% of live splenocytes. STAT5 deletion. (open shapes) % ROSA + % floxed
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Supplementary Data Table of Contents:
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Supporting Information
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Inhibition of Pulmonary Anti Bacterial Defense by IFN γ During Recovery from Influenza Infection By Keer Sun and Dennis W. Metzger Supplementary Figures d a Ly6G Percentage survival f 1 75 5 1 25 1 5 1
Canberra, Australia). CD11c-DTR-OVA-GFP (B6.CD11c-OVA), B6.luc + and. Cancer Research Center, Germany). B6 or BALB/c.FoxP3-DTR-GFP mice were
Supplemental Materials and Methods Mice Female C57BL/6 (B6, I-E null, H-2 b ), BALB/c (H-2 d ) + ), FVB/N (H-2 q, I-E null, CD45.1 + ), and B6D2F1 (H-2 b/d ) mice were purchased from the Animal Resources
Supplemental Figure 1. IL-3 blockade with Fab CSL362 depletes plasmacytoid dendritic cells (pdcs), but not basophils, at higher doses.
Supplemental Figure 1. IL-3 blockade with Fab CSL362 depletes plasmacytoid dendritic cells (pdcs), but not basophils, at higher doses. Percentage of viable (A) pdcs (Sytox Blue-, Lin1-, HLADR+, BDCA2++)
B6/COLODR/SPL/11C/83/LAP/#2.006 B6/COLODR/SPL/11C/86/LAP/#2.016 CD11C B6/COLODR/SPL/11C/80/LAP/#2.011 CD11C
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Supplementary Table 1
Supplementary Table 1 Flow Cytometry Antibodies Antibody Fluorochrome Clone Vendor CD45 PE-cyanine 7 30-F11 D ioscience CD3 Pacific lue 17A2 iolegend (San Diego, CA) CD11b APC M1/70 iolegend (San Diego,
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Supplementary Figure 1 IL-27 IL
Tim-3 Supplementary Figure 1 Tc0 49.5 0.6 Tc1 63.5 0.84 Un 49.8 0.16 35.5 0.16 10 4 61.2 5.53 10 3 64.5 5.66 10 2 10 1 10 0 31 2.22 10 0 10 1 10 2 10 3 10 4 IL-10 28.2 1.69 IL-27 Supplementary Figure 1.
Nature Immunology: doi: /ni.3836
Supplementary Figure 1 Recombinant LIGHT-VTP induces pericyte contractility and endothelial cell activation. (a) Western blot showing purification steps for full length murine LIGHT-VTP (CGKRK) protein:
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Title. CitationCancer science, 109(7): Issue Date Doc URL. Rights(URL)
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