Medical School Histology Basics. VIBS 289 lab. Eye
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1 Medical School Histology Basics VIBS 289 lab Eye Larry Johnson Texas A&M University
2 Aqueous humor OUTLINE OVERVIEW CELLULAR STRUCTURES THROUGH WHICH LIGHT PASSES A. CORNEA B. LENS C. RETINA STRUCTURES WHICH INFLUENCE THE IMAGE A. IRIS B. CILIARY BODIES C. TRABECULAR MESHWORK Vitreous humor
3 Aqueous humor VITREOUS BODY (VITREOUS HUMOR)
4 34412
5 192 Eye conjuctiva on white of eye sclera Conjunctiva Stratified squamous epithelium with some goblet cells
6 Eye 192 human and monkey Connective tissue Aqueous humor Nerve 192 Posterior chamber Retina Cornea Optic nerve Sclera Pupil Anterior chamber Lens Iris Vitreous body Chorioid layer Ciliary body
7
8 192 Eye corneal layers Stratified squamous Epithelium Bowman's membrane Corneal stroma Corneal endothelium Corneal fibroblasts Descemet's Membrane
9 292 corneal layers Iris of Eye (toluidine blue) Ciliary muscle: smooth muscle Sclera Lens Sclera-cornea junction (limbus) Bowman's membrane Aqueous humor Corneal stroma Corneal endothelium Descemet's Membrane Anterior chamber Iris Posterior chamber Iris Aqueous humor
10 292 Ciliary muscle: smooth muscle Ciliary processes Blood vessel lens Nerves Ciliary muscle 192
11 192 Eye ciliary muscle Pupil Iris Artifactual space Conjunctival sac Sclera-cornea junction (limbus) Ciliary muscle Posterior chamber Ciliary processes Anterior chamber Ciliary muscle (smooth muscle)
12 Ciliary processes Eye secretory cells Pigmented cells of the ciliary processes become the dilator muscle of the iris Pigmented cells Secretory cells Iris Secretory cells become the pigmented cells of the iris
13 292 Conjunctiva Eye (toluidine blue) Iris Ciliary muscle Air Trabecular meshwork Canal of Schlemm Limbus Anterior chamber Endothelium of trabecular meshwork is continuous with the transport endothelium of the cornea Iris Posterior chamber Ciliary processes
14 192 Eye trabecular meshwork and canal of Schlemm Limbus Canal of Schlemm Ciliary muscle Trabecular meshwork Anterior chamber with aqueous humor
15 Canal of Schlemm Trabecular meshwork Iris Blood vessels Posterior chamber with aqueous humor Monkey eye 34412
16 292 Eye (toluidine blue) Lens Dilator muscle Iris pupil Iris Lens Constrictor muscle Pupil Cuboidal epithelium on anterior surface of lens Iris
17 292 Eye (toluidine blue) Lens Lens cuboidal epithelium Lens capsule Lens Iris constrictor smooth muscle
18 The capsule is a shared basement membrane of the lens epithelium
19 The capsule is a shared basement membrane of the lens epithelium Lens
20 192 and Lens and iris Pigmented epithelium of iris Lens anterior surface 192 Iris Lens capsule Iris Lens cuboidal epithelium Iris constrictor muscle is smooth muscle
21 292 Eye (toluidine blue) elongation of lens cuboidal cells at bow region Capsule Lens cuboidal epithelium Lens cuboidal epithelium on its anterior surface gives rise to elongated lens fibers in the bow region, and the shared basement membrane of these cells is the capsule Capsule flattened lens fibers Artifactual space No cuboidal epithelium on the posterior surface of the lens and the capsule is thinner there
22 34412 Eye monkey Sites of attachment of zonules to the lens capsule Anterior surface Iris lens Ligaments (zonules) lens 192 One site of attachment of a zonule to the capsule on the lens on its posterior surface
23 Sclara Zonules Ciliary muscle 192 Lens fibers Capsule and cuboidal epithelium on the anterior surface of lens Thinner capsule on the posterior surface of the lens
24 Eye monkey Capillary network in retina Pigmented layer of epithelial cells Elaborate network of vessels, the choroid plexus. Central artery of the retina
25
26 34412 Vitreous body Ganglion cell layer Cones Rods Bipolar cells Retinal pigmented epithelium Photosensitive portion of rod and cone cells Retinal pigmented epithelium Sclera Venule in the choroid plexus
27 Rods Cones Photosensitive portion of rod and cone cells Retinal pigmented epithelium Nuclei of rod and cone cells = outer nuclear layer
28 Eye - Retina and choroid: Blood vessels in the retina Artifact space 292 Choroid Choroid plexus Melanin granules Retina Nucleus Retinal pigment epithelium
29 292 Retina (toluidine blue) Bipolar cells Photoreceptor cells Artifactual space Rods Optic nerve Capillary Cones
30 Typical retina Ganglion cell layer Bipolar cells Macula with top two layers pulled back to aid vision Rods and cones Rods and cones Retinal pigmented epithelium Retinal pigmented epithelium
31 Conjunctiva Cornea 192 lens Cornea Iris Choroid plexus Vitreous body Retina Sclera Lens 292 Anterior chamber Posterior chamber Optic nerve Aqueous humor
32 34412 Pigmented epithelium Choroid Blind spot where optic nerve enters the retina Photosensitive region of rods and cones Bipolar cell layer Ganglion cell layer
33 292 Eye (toluidine blue) Blind spot where optic nerve enters the retina
34 34412 Choroid plexus Retina OPTIC CUP AND NERVE HEAD Retina
35 In summary
36 3. Which function(s) least match(s) that of the cornea? a. protection b. structural support c. filter out undesirable light rays d. focus image on photosensitive portion of the photoreceptor cells e. none of the above Questions on the eye 1. The integrity of the relationship among structures of the eye is produced/maintained by: a. supporting layer of the eye holding pressure b. precise regulation of pressure via aqueous humor production and outflow c. the bony structure of the sclera in mammals d. a and b e. a, b, and c 2. Which structure is not correctly paired with its function? a. the iris regulates the amount of light that reaches the retina b. ciliary bodies alter the shape of the lens by the attached zonules c. the trabecular meshwork enhances the outflow of vitreous humor d. the cornea focuses the image on the retina e. the retinal and choroid blood vessels provide nutrients to the retina
37 Many illustrations in these VIBS Histology YouTube videos were modified from the following books and sources: Many thanks to original sources! Bruce Alberts, et al Molecular Biology of the Cell. Garland Publishing, Inc., New York, NY. Bruce Alberts, et al Molecular Biology of the Cell. Garland Publishing, Inc., New York, NY. William J. Banks, Applied Veterinary Histology. Williams and Wilkins, Los Angeles, CA. Hans Elias, et al Histology and Human Microanatomy. John Wiley and Sons, New York, NY. Don W. Fawcett Bloom and Fawcett. A textbook of histology. W. B. Saunders Company, Philadelphia, PA. Don W. Fawcett Bloom and Fawcett. A textbook of histology. Chapman and Hall, New York, NY. Arthur W. Ham and David H. Cormack Histology. J. S. Lippincott Company, Philadelphia, PA. Luis C. Junqueira, et al Basic Histology. Lange Medical Publications, Los Altos, CA. L. Carlos Junqueira, et al Basic Histology. Appleton and Lange, Norwalk, CT. L.L. Langley, et al Dynamic Anatomy and Physiology. McGraw-Hill Book Company, New York, NY. W.W. Tuttle and Byron A. Schottelius Textbook of Physiology. The C. V. Mosby Company, St. Louis, MO. Leon Weiss Histology Cell and Tissue Biology. Elsevier Biomedical, New York, NY. Leon Weiss and Roy O. Greep Histology. McGraw-Hill Book Company, New York, NY. Nature ( Vol. 414:88,2001. Arthur C. Guyton,1971.Textbook of Medical Physiology W.B. Saunders company, Philadelphia, PA WW Tuttle and BA Schottelius 1969 Textbook of Physiology C.V. Mosby Co. A.L. Mescher 2013 Junqueira s Basis Histology text and atlas, 13 th ed. McGraw
38
39 The end of
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