Molecular Remission Induction with Retinoic Acid and Anti-CD33 Monoclonal Antibody HuM195 in Acute Promyelocytic Leukemia 1

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1 372 Vol. 6, , February 2000 Clinical Cancer Research Molecular Remission Induction with Retinoic Acid and Anti-CD33 Monoclonal Antibody HuM195 in Acute Promyelocytic Leukemia 1 Joseph G. Jurcic, 2 Tony DeBlasio, Larry Dumont, Tzy-Jyun Yao, and David A. Scheinberg Leukemia Service, Departments of Medicine and Biostatistics, Memorial Sloan-Kettering Cancer Center and Cornell University Medical College, New York, New York [J. G. J., T. D., T-J. Y., D. A. S.], and Protein Design Labs, Inc., Fremont, California [L.D.] ABSTRACT Despite achieving complete remission with retinoic acid (RA), most patients with acute promyelocytic leukemia (APL) have minimal residual disease detectable by reverse transcription-pcr (RT-PCR) amplification. HuM195, a humanized monoclonal antibody reactive with the cell surface antigen CD33, specifically targets and kills myeloid leukemia cells. We studied whether HuM195 could eliminate minimal residual disease in patients with APL by using RT-PCR. After attaining clinical complete remission with RA and/or chemotherapy, patients received HuM195 twice weekly for 3 weeks. Patients in first remission were given consolidation chemotherapy, generally with three cycles of idarubicin and cytarabine. Patients in second or greater remission did not receive chemotherapy. All patients received six monthly courses of maintenance with two doses of HuM195. Twenty-five of 27 patients treated in first remission had positive RT-PCR determinations before HuM195 treatment. Of the 22 patients evaluable for conversion of positive RT-PCR assays, 11 (50%) became RT-PCR negative after HuM195 treatment without additional therapy. Within the subset of patients who received RA alone as induction, 8 of 18 evaluable patients (44%) had negative RT-PCR determinations after HuM195 treatment but before chemotherapy. Among similar patients treated on earlier studies, 7 of 34 patients (21%) induced into remission with RA and then maintained on the drug for 1 month were Received 5/14/99; revised 10/12/99; accepted 10/27/99. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. 1 Supported in part by Grants FD-R and FD-R from the Orphan Products Division, Food and Drug Administration; NIH Grants RO1 CA55349 and PO ; and Grant EDT-47 from the American Cancer Society. J. G. J. is the recipient of a Clinical Oncology Career Development Award from the American Cancer Society. D. A. S. is a Translational Investigator of the Leukemia Society of America. 2 To whom requests for reprints should be addressed, at Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY Phone: (212) ; Fax: (212) RT-PCR negative before chemotherapy (P 0.07). Twentyfive of 27 patients with newly diagnosed APL (93%) remain in clinical complete remission for 7 to 58 months, with median follow-up of 29 months. Seven patients in second or third remission and one patient in molecular relapse were also treated. Only one of these patients became RT-PCR negative after treatment with HuM195. These data suggest that HuM195 has activity against minimal residual disease in APL, particularly in newly diagnosed patients. INTRODUCTION APL 3 is characterized by a specific translocation that fuses the PML gene on chromosome 15 with a RAR gene (RAR- )on chromosome 17 (1 3). Detection of PML/RAR- fusion mrna by RT-PCR amplification is useful in establishing the diagnosis of APL, predicting response to therapy, and predicting relapse (4 12). All-trans-RA or 9-cis-RA induces remission in up to 95% of patients, most of whom have minimal residual disease detectable by RT-PCR before receiving additional therapy (13 16). Therefore, serial monitoring of bone marrow using RT- PCR enables the effect of postremission therapy to be assessed in patients who are in clinical complete remission but remain at risk for relapse. M195 is a mouse monoclonal antibody that binds CD33, a cell surface glycoprotein found on most myeloid leukemias and clonogenic leukemia progenitors (17, 18). Although CD33 is expressed on committed myelomonocytic and erythroid progenitor cells, it is not found on mature granulocytes or nonhematopoietic tissues (19, 20). Early trials showed that M195 rapidly targets leukemia cells in patients and that 131 I-labeled M195 can eliminate large leukemic burdens (21 23). Lower doses of 131 I- labeled M195 had activity against minimal residual disease in patients with APL in second remission (24). However, this therapy was limited by myelosuppression due to the nonspecific cytotoxicity of 131 I and by formation of human antimouse antibodies that prevented repeated dosing. Humanized M195 (HuM195), constructed by grafting complementarity-determining regions of murine M195 into a human IgG1 framework and backbone, mediates leukemia cell killing in vitro by human peripheral blood mononuclear cells (25, 26). It displayed rapid targeting of leukemia cells and a pharmacology similar to that of murine M195 in a Phase I trial, but without significant immunogenicity, thereby allowing the administration of multiple doses (27). Treatment with supersaturating doses of native HuM195 produced a complete remission 3 The abbreviations used are: APL, acute promyelocytic leukemia; RA, retinoic acid; RT-PCR, reverse transcription-pcr; RAR, retinoic acid receptor; HAHA, human antihuman antibody.

2 Clinical Cancer Research 373 in 1 of 10 patients with advanced myeloid leukemias and reduced the percentage of bone marrow blasts in another 3 patients (28). We studied the ability of HuM195 to eliminate minimal residual disease detectable by RT-PCR in patients with APL. MATERIALS AND METHODS Eligibility. Patients with APL were eligible for treatment with HuM195 after achieving a clinically documented complete remission by conventional criteria (29) after induction with RA and/or chemotherapy. Initially, newly diagnosed patients were enrolled on study within 2 weeks after attaining a clinical complete remission, before the administration of consolidation chemotherapy. After the first 16 patients, the protocol was modified to permit up to two courses of consolidation chemotherapy before entering the study. Patients in clinical complete remission but with evidence of disease detectable by RT-PCR were also eligible. Treatment. On entering the study, patients received 3 mg/m 2 HuM195 by i.v. infusion over a min period twice weekly for six doses. Acetaminophen and diphenhydramine were given before each treatment to prevent infusion-related toxicity. Patients enrolled after induction with RA alone received three cycles of consolidation chemotherapy. The first cycle consisted of 200 mg/m 2 /day cytarabine by continuous i.v. infusion for 5 days and 12 mg/m 2 idarubicin i.v. for 3 days. Subsequent courses were repeated every 6 8 weeks and consisted of cytarabine for 4 days and idarubicin for 2 days at the same doses. Patients entering the study after induction with concomitant RA and chemotherapy received only the second and third chemotherapy courses as described above. Patients enrolled after the administration of consolidation therapy received additional cycles of chemotherapy so that a total of three courses were given. After the completion of chemotherapy, six monthly courses of maintenance with two doses of HuM195 given 3 4 days apart were administered. Patients in second or greater remission or in molecular relapse were treated with six doses of HuM195 over 3 weeks as described above. After this therapy, appropriate candidates proceeded to bone marrow or peripheral blood progenitor cell transplantation. The remaining patients received maintenance with HuM195 as described above. These patients did not receive additional consolidation chemotherapy because they relapsed after standard treatment. One patient in second remission was treated on an earlier protocol in which maintenance with HuM195 was continued for 1 year. Patients were treated on protocols approved by the Institutional Review Board and the Food and Drug Administration after informed consent was obtained. RT-PCR Analysis. Serial bone marrow aspirates were analyzed using previously described RT-PCR techniques to detect PML/RAR- rearrangements with a sensitivity of approximately 1 in 10 4 cells (5, 9). We examined samples before patients began treatment with HuM195, after initial antibody therapy, after each consolidation chemotherapy course, and periodically thereafter. Plasma HuM195 Levels and HAHA Response. Plasma levels of HuM195 were measured before and 5 min after each HuM195 infusion by an ELISA. This assay uses a version of the double-antibody sandwich technique with a high-affinity mouse anti-idiotypic antibody to M195 (30). We used a previously described double antigen ELISA to detect HAHA (27). Specificity of HAHA was assessed by competition of the patient s serum with a panel of monoclonal antibodies sharing various regions of homology with HuM195 and unrelated controls. Historical Controls and Statistical Analysis. We compared newly diagnosed patients entered on this study with a group of historical control patients treated at Memorial Hospital immediately before the initiation of the current trial. This group consisted of 50 consecutively diagnosed patients with APL who attained clinical complete remission with all-trans-ra (n 49) or 9-cis-RA (n 1) and then received consolidation chemotherapy, generally with three courses of cytarabine and idarubicin, as described above (16, 31, 32). The median follow-up duration is 62 months (range, months). The 2 test was used to compare molecular remission rates between groups. To ensure that these groups were comparable, we included patients who received induction solely with RA for this analysis. The Kaplan-Meier method was used to determine the probability of disease-free survival. Comparison of disease-free survival duration between groups was performed using the log-rank test. For uniformity among groups, this analysis was limited to patients induced with RA followed by either two or three cycles of consolidation chemotherapy. RESULTS Patient Characteristics. Thirty-five patients (median age, 45 years) were enrolled between October 1994 and March 1999 (Table 1). Among the 27 patients treated in first remission, 21 patients received induction with either all-trans-ra (n 18) or 9-cis-RA (n 3) alone. Five patients received all-trans-ra concomitantly with chemotherapy as induction. One patient (patient 24) received chemotherapy alone as an induction. Only two patients (patients 24 and 26) were given consolidation chemotherapy before entering the study. The characteristics of the historical control group are compared with the newly diagnosed patients treated on the current study in Table 2. These groups are comparable in all respects except that patients treated on the current study received fewer cycles of consolidation therapy than patients in the historical group. This is a result of a greater number of patients receiving induction with concomitant RA and chemotherapy in the current study. Among the seven patients treated in second or third remission, five received induction with all-trans-ra (n 2) or 9-cis-RA (n 3), and two received concomitant all-trans-ra and chemotherapy. Patient 15 was treated in molecular relapse 35 months after completing therapy with all-trans-ra followed by three courses of chemotherapy. Molecular Remission Induction. Among the 27 patients treated in first remission, 25 had minimal residual disease detectable by RT-PCR before receiving HuM195. One of these patients (patient 24) received consolidation chemotherapy before enrolling on study. Patients were considered evaluable for response only if adequate RNA samples were obtained for RT-PCR analysis. After six doses of HuM195, 11 of the 22

3 Table 1 Patient characteristics Patient no. Age/sex (yr) Remission status Previous therapy Current induction Time to remission (wks) Consolidation chemotherapy Before HuM195 After HuM195 Cytogenetics 1 55/F CRI a NA ATRA 6 None IDR/Ara-C 3 t(15;17), 8 Short 2 36/F CR2 ATRA/IDR/Ara-C 9-cis-RA 4 None None Normal Long 3 20/F CR1 NA ATRA 8 None IDR/Ara-C 3 t(15;17) Short 4 56/M CR1 NA ATRA 10 None IDR/Ara-C 3 t(15;17) Long 5 68/M CR1 NA ATRA 6 None IDR/Ara-C 3 t(15;17) Long 6 31/F CR1 NA ATRA 4 None IDR/Ara-C 3 t(15;17) Long 7 60/M CR2 ATRA/IDR/Ara-C 9-cis-RA 8 None b t(15;17;5) Short 8 46/F CR1 NA 9-cis-RA 8 None IDR/Ara-C 3 t(15;17) Long 9 46/F CR1 NA ATRA 5 None IDR/Ara-C 3 t(15;17), 8, del(9) Long 10 36/F CR1 NA 9-cis-RA 5 None IDR/Ara-C 3 t(3;15;17) Long 11 25/F CR2 DNR/Ara-C; HiDAC 9-cis-RA 4 None None t(15;17) Long 12 45/F CR2 DNR/Ara-C ATRA 7 None c t(15;17) Short 13 45/M CR3 DNR/Ara-C; ATRA/IDR/ Ara-C; 9-cis-RA ATRA/MTX/vinorelbine/ fludarabine 7 None 14 30/M CR2 IDR/Ara-C ATRA 7 None c c PML/RAR- isoform Not done Short t(15;17) Short Long f 15 48/M Molecular relapse ATRA/IDR/Ara-C NA NA None None t(15;17), del(3), 8, 16, mar 16 46/F CR1 NA 9-cis-RA 8 None IDR/Ara-C 3 Normal Long 17 41/M CR1 NA ATRA 8 None IDR/Ara-C 3 t(15;17) Long 18 17/F CR1 NA HiDAC/DNR/6TG/ATRA 6 None IDR/Ara-C 3 t(15;17) Short 19 36/F CR1 NA ATRA 6 None IDR/Ara-C 3 t(15;17) Long 20 65/F CR1 NA ATRA 6 None IDR/Ara-C 2 d t(15;17) Long 21 37/M CR1 NA ATRA 8 None IDR/Ara-C 3 t(15;17) Long 22 18/M CR1 NA ATRA 9 None IDR/Ara-C 3 t(15;17) Short 23 36/F CR1 NA ATRA/IDR/Ara-C 6 None IDR/Ara-C 2 t(15;17) Short 24 45/M CR1 NA IDR/Ara-C 4 IDR/Ara-C 1 e t(15;17) Short 25 55/F CR1 NA ATRA 6 None IDR/Ara-C 3 t(15;17) Short 26 39/M CR1 NA ATRA/IDR/Ara-C 4 IDR/Ara-C 2 None t(15;17) Long 27 35/F CR1 NA ATRA/IDR 5 None IDR/Ara-C 2 t(15;17) Long 28 60/F CR1 NA ATRA 7 None IDR/Ara-C 3 Failure Short 29 50/M CR1 NA ATRA 5 None IDR/Ara-C 3 t(15;17) Long 30 43/F CR2 ATRA/IDR/Ara-C ATRA/DNR/Ara-C 4 None b t(15;17) Long 31 66/M CR1 NA ATRA 8 None IDR/Ara-C 3 t(15;17), 8 Long 32 71/M CR1 NA ATRA/Ara-C 8 None f t(15;17) Long 33 66/M CR1 NA ATRA 8 None IDR/Ara-C 3 t(15;17) Long 34 36/M CR1 NA ATRA 4 None IDR/Ara-C 3 Normal Short 35 48/F CR1 NA ATRA 8 None IDR/Ara-C 2 t(15;17) Long a CR1, first complete remission; CR2, second complete remission; CR3, third complete remission; NA, not applicable; ATRA, all-trans-ra; IDR, idarubicin; DNR, daunorubicin; Ara-C, cytarabine; HiDAC, high-dose cytarabine; MTX, methotrexate; 6TG, 6-thioguanine. b Patient removed from study; received chemotherapy for persistent positive RT-PCR assay. c Patient removed from study; underwent autologous bone marrow transplant. d Patient received only two cycles of consolidation due to severe chemotherapy-related toxicity. e Patient removed from study; received consolidation with ATRA/IDR. Patient removed from study; unable to receive IDR due to cardiomyopathy. 374 Molecular Remission with HuM195 in APL

4 Clinical Cancer Research 375 Table 2 Characteristics of the study and historical control groups Current study (n 27) Historical control group (n 50) P Age (yr) Median Range Sex Male Female PML/RAR- isoform type Long Short 9 15 Induction therapy RA RA chemotherapy 5 d 0 Chemotherapy only 1 d 0 No. of consolidation courses 0 1 d d d d Time to remission (wks) Median Range a Unpaired t test. b Fisher s exact test. c 2 test. d Excluded from analysis (see text) a 0.63 b 1.0 b 1.0 c 0.01 a 0.54 a evaluable patients (50%) converted to PCR negative before receiving any further therapy (Fig. 1A). Subsequently, all remaining evaluable patients became RT-PCR negative with either one (n 9) or two (n 1) courses of consolidation chemotherapy. Among the eight patients with relapsed APL, only one patient treated in second remission (patient 2) became RT-PCR negative after HuM195 treatment without any additional therapy (Fig. 1B). HuM195 maintenance was continued for 1 year. After 18 months, this patient tested positive for the PML/RAR- rearrangement but remained in clinical remission. She failed to respond to additional HuM195 and relapsed clinically 1 month later. Comparison of Molecular Remission Induction Rates with and without HuM195. To compare the molecular remission rates of patients treated with or without HuM195, we limited the analysis to patients induced with RA alone. In the current study, 21 patients received HuM195 after RA induction followed by consolidation chemotherapy. Among the historical control group, 43 patients were induced into remission with RA and then maintained on the drug for 1 month before receiving consolidation therapy. Patients were evaluable for response only if adequate RNA samples were obtained for analysis. At the time of clinical complete remission, 1 of the 21 patients (5%) in the current study and 3 of the 40 evaluable patients (8%) in the historical group were RT-PCR negative. One month after achieving clinical remission with RA, 8 of 18 evaluable patients (44%) in the current trial were RT-PCR negative after receiving HuM195 but before the administration of chemotherapy (Fig. 2A). In previous trials, 7 of 34 patients (21%) who continued RA therapy alone for 1 month after attaining clinical remission tested negative (P 0.07; Fig. 2B). All 19 evaluable patients (100%) treated on this study had negative RT-PCR determinations after RA induction followed by HuM195 and one course of consolidation chemotherapy (Fig. 2A). These results are comparable to the 36 of 40 patients (90%) who were RT-PCR negative after RA induction and three consolidation courses in earlier studies (Fig. 2B). Remission and Survival Duration. Twenty-five of 27 patients (93%) with newly diagnosed APL remain in remission with a median follow-up duration of 29 months (range, 7 58 months) from the time of clinically documented complete remission. Two patients (patients 21 and 22) relapsed after 16 and 20 months, respectively. The estimated probability of remaining disease free at 29 months for all newly diagnosed patients treated with RA, HuM195, and chemotherapy is 89%. We compared the probability of disease-free survival for the 21 patients in the current study, who received induction with RA followed by postremission HuM195 and then two or three cycles of consolidation therapy, with that of 47 patients in the historical control group, who were induced with RA and then received two or three cycles of consolidation chemotherapy. The estimated disease-free survival after 29 months for this subset of study patients is 86%, and the estimated disease-free survival is 76% for the patients in the historical control group (P 0.22; Fig. 3). Twenty-six of the 27 patients (96%) treated on the current study are alive, with a median follow-up duration of 30 months (range, 8 60 months) from the time of diagnosis. The estimated probability of survival at 30 months is 93%. One patient died with relapsed APL 26 months after diagnosis. Among the eight patients treated in second or third remission or in molecular relapse, five patients relapsed clinically after 4 27 months. Four of these patients achieved remission with additional therapy and remain disease free. Patient 11 died with relapsed APL after 15 months. The remaining three patients underwent autologous bone marrow transplantation after they failed to achieve molecular remission with six infusions of HuM195. The disease-free and overall survival times of this group range from 4 32 months and 6 56 months, respectively, with a median follow-up duration of 31 months. A previous series of relapsed patients treated without HuM195 had a median disease-free survival duration of 3 11 months (24). Adverse Effects. HuM195 infusions were generally associated with few or no side effects (Table 3). The most common adverse reactions included low-grade fever, nausea, and chills and were usually seen within 2 h after completion of the infusion and only after the first dose. These reactions were effectively treated with acetaminophen, diphenhydramine, and meperidine. Therapy was administered entirely in the outpatient setting. Mild neutropenia (usually grade 1) and thrombocytopenia (grade 1) lasting for 3 7 days occurred in six patients, likely because of CD33 expression by normal hematopoietic progenitor cells. No episodes of neutropenic fever were observed. Five patients also developed transient postural hypotension requiring the administration of i.v. fluids. HAHA Response. Patient 2 developed an immune response to HuM195 9 weeks after beginning therapy. A compe-

5 376 Molecular Remission with HuM195 in APL Fig. 1 Longitudinal RT-PCR results from bone marrow aspirates of 27 patients with newly diagnosed APL treated with postremission HuM195 and cytotoxic therapy (A) and eight patients in second or third remission or molecular relapse treated with HuM195 (B). Each circle represents a RT-PCR assay performed at the indicated time after achieving clinical complete remission. Only samples with adequate RNA quality for amplification of control RNA are included. F, a positive result; E, a negative result. p, treatment with HuM195; f, treatment with chemotherapy. Arrows, the time of clinical relapse. tition assay to assess the specificity of HAHA showed suppression of the signal with only murine M195 and HuM195, indicating a pure anti-idiotypic response. HAHA levels increased over 10 weeks to an anti-idiotypic equivalent concentration of ng/ml. Because of the low level of immune response, serum levels of HuM195 were unaffected, and this patient was able to complete 1 year of maintenance therapy. Peak serum levels of HuM195 were g/ml before the development of HAHA and g/ml after a HAHA response was detected. DISCUSSION Elimination of minimal residual disease detectable by RT- PCR for the PML/RAR- fusion transcript appears necessary for patients with APL to achieve long-term remissions (8 12,

6 Clinical Cancer Research 377 Fig. 2 Proportion of patients with newly diagnosed APL achieving molecular remission after treatment with RA alone, RA followed by HuM195, and RA followed by HuM195 and a single course of consolidation chemotherapy in this study (A) and after treatment with RA alone and RA followed by up to three courses of chemotherapy in previous trials (B). 33). After induction of clinical remission with RA alone, however, the majority of patients will test positive for the PML/ RAR- rearrangement, and without additional therapy, all patients will relapse. In our historical group, only 8% of patients treated solely with RA were RT-PCR negative at the time clinical complete remission was first documented. When therapy with RA was continued for 1 month after attaining clinical remission, 21% of these patients achieved molecular remission. In the current study, therapy with RA followed by HuM195 given during the month after clinical complete remission was documented produced molecular remissions in 44% of patients. This result compares favorably with the molecular remission rate achieved using RA alone in the historical group with a statistical significance of P A prospective, randomized trial with greater patient numbers will ultimately be needed to prove a significant improvement in molecular remission rates with the addition of HuM195 to RA. Using a similar strategy, Mandelli et al. (34) reported that induction with combined all-trans-ra and idarubicin produced molecular remissions in 61% of patients before the administration of consolidation therapy. At least 90% of newly diagnosed patients with APL will become RT-PCR negative after receiving RA induction followed by multiple courses of consolidation chemotherapy (8, 9, 11). In this study, all 19 evaluable patients (100%) tested RT- PCR negative after RA induction, HuM195 treatment, and one course of consolidation chemotherapy. This is comparable to the 90% of patients treated on earlier studies who were RT-PCR negative after RA induction and three intensive consolidation courses with combination chemotherapy. Similarly, Diverio et al. (12) reported that 96% of patients achieved a molecular remission after induction with all-trans-ra and idarubicin followed by three cycles of consolidation chemotherapy. These data suggest that postremission HuM195 could potentially reduce the number of consolidation chemotherapy courses required to achieve long-term remissions and thereby decrease therapy-related morbidity. After treatment with RA and chemotherapy, approximately 75% of patients with newly diagnosed APL will achieve longterm remissions (31, 32, 34 36). Among patients treated with all-trans-ra induction followed by two cycles of consolidation chemotherapy, Tallman et al. (37) report the estimated probability of disease-free survival after 1, 2, and 3 years to be 87%, 70%, and 67%, respectively. Mandelli et al. (34) report 1- and 2-year disease-free survival rates of 83% and 79% for patients induced with combined all-trans-ra and idarubicin followed by three cycles of chemotherapy. Fenaux et al. (38) noted an event-free survival rate of 77% at 2 years for patients treated with all-trans-ra induction followed by two courses of consolidation chemotherapy and 84% for patients treated with concomitant all-trans-ra and chemotherapy as induction followed by two cycles of consolidation. Among patients treated in earlier studies with RA induction and consolidation chemotherapy at our institution, the estimated probabilities of disease-free survival at 1, 2, and 3 years were 86%, 80%, and 76%, respectively. In the current study for all newly-diagnosed patients treated with RA, HuM195, and chemotherapy, the 1-, 2-, and 3-year diseasefree survival rates were 100%, 89%, and 89%, respectively. Although not statistically significant, these results suggest that the addition of HuM195 to postremission chemotherapy may extend disease-free survival times. Larger patient numbers, a longer follow-up duration, and prospective randomized trials will be necessary to prove a statistically significant benefit to this approach. In general, biological therapies designed to act on minimal disease require large randomized trials to evaluate their effects. Because APL is characterized by a detectable molecular marker whose presence directly correlates with relapse, this disease serves as a model in which one can assess the activity of immunotherapy in a relatively brief period of time using RT- PCR techniques. In this study, 50% of the patients with newly diagnosed APL who had positive RT-PCR determinations before receiving HuM195 became negative after antibody therapy alone. These data suggest that HuM195 has activity against minimal residual disease in APL and that its use overcomes the nonspecific myelosuppression and immunogenicity seen in earlier studies with 131 I-labeled M195 (22 24). Moreover, these results support the investigation of postremission therapy with

7 378 Molecular Remission with HuM195 in APL Fig. 3 Probability of disease-free survival for patients with newly diagnosed APL treated with RA induction followed by postremission HuM195 and chemotherapy in this study or treated with RA followed by chemotherapy alone in previous trials. Tick marks, the time of last follow-up. Table 3 Toxicities of HuM195 infusions Toxicity No. of patients (n 35) Fever 16 Grade 1 9 Grade 2 7 Nausea/vomiting (grade 1) 13 Chills/rigors 13 Hematological toxicity Neutropenia 6 Grade 1 4 Grade 2 1 Grade 3 1 Thrombocytopenia (grade 1) 1 Hypotension (grade 2) 5 Rash/flushing 5 Headache 3 Abdominal pain/back pain 3 Bone pain/myalgias 1 Chest tightness 1 HuM195 in other subtypes of acute myelogenous leukemia and myeloid leukemias in general, where molecular markers of residual disease may not be available. The effect of maintenance therapy with HuM195 after the completion of chemotherapy is difficult to assess without a randomized trial because the molecular marker for residual disease is almost always undetectable after completion of consolidation chemotherapy. Nevertheless, Fenaux et al. (38) and Tallman et al. (37) have reported that maintenance with RA and/or chemotherapy prolongs remission duration in patients with newly diagnosed APL. These studies provide the rationale for the study of HuM195 in the postchemotherapy setting. Only one molecular remission was observed among eight patients (13%) with APL in second or greater remission, suggesting that the optimal time for treatment with HuM195 may be in newly diagnosed disease. No differences in antigen expression were noted among the relapsed patients. The lower molecular remission rate in relapsed patients may be explained in part by a quantitative difference in the level of residual APL in patients with newly diagnosed and relapsed disease after remission induction. Additionally, resistance to immunologically mediated mechanisms of cytotoxicity may account for the difference in molecular remission rates. In vitro studies showed that multidrug resistance cell lines created by continuous selection with vincristine or by retroviral infection were resistant to complement-mediated cytotoxicity by HuM195. This resistance was found to be related to an elevated intracellular ph observed in the multidrug resistance cell lines (39). This study suggests that HuM195 has activity against minimal residual disease in APL. Multiple doses of HuM195 can be administered safely to patients after remission induction and appear to eliminate minimal residual disease detectable by RT- PCR in some patients. The use of postremission HuM195 after induction with RA could potentially reduce the number of consolidation chemotherapy courses required for long-term remissions. Combinations of HuM195 with other active agents, such as arsenic trioxide (40), may further decrease or eliminate the need for consolidation chemotherapy in APL. This study provides the rationale for monoclonal antibody-based therapy for residual or reduced disease in various malignancies. ACKNOWLEDGMENTS We thank Drs. Man Sung Co and Cary Queen (Protein Design Labs, Inc., Fremont, CA) for providing HuM195; Lucy Dantis and her staff for expert research nursing; Tatyana Korontsvit for laboratory assistance; Deci Tyson, Doran Ursan, and Jenny Jimenez for assistance in data management; Dr. Stephen D. Nimer for support of the Minimal Residual Disease Laboratory; Dr. Colin Begg for biostatistical help; and Dr. Raymond P. Warrell, Jr. for direction of the RA studies in APL (Memorial Sloan-Kettering Cancer Center, New York, NY). REFERENCES 1. de Thé, H., Chomienne, C., Lanotte, M., Degos, L., and Déjean, A. The t(15;17) translocation of acute promyelocytic leukaemia fuses the retinoic acid receptor gene to a novel transcribed locus. Nature (Lond.), 347: , 1990.

8 Clinical Cancer Research Kakizuka, A., Miller, W. H., Jr., Umesono, K., Warrell, R. P., Jr., Frankel, S. R., Murty, V. V. V. S., Dmitrovsky, E., and Evans, R. M. Chromosomal translocation t(15;17) in human acute promyelocytic leukemia fuses RAR- with a novel putative transcription factor, PML. Cell, 66: , de Thé, H., Lavau, C., Marcio, A., Chomienne, C., Degos, L., and Déjean A. The PML-RAR- fusion mrna generated by the t(15;17) translocation in acute promyelocytic leukemia encodes a functionally altered RAR. Cell, 66: , Biondi, A., Rambaldi, A., Alcalay, M., Pandolfi, P. P., LoCoco, F., Diverio, D., Rossi, V., Mencarelli, A., Longo, L., Zangrilli, D., Masera, G., Tiziano, B., Mandelli, F., Grignani, F., and Pelicci, P. G. RAR- rearrangements as a genetic marker for diagnosis and monitoring in acute promyelocytic leukemia. Blood, 77: , Miller, W. H., Jr., Kakizuka, A., Frankel, S. R., Warrell, R. P., Jr., DeBlasio, A., Levine, K., Evans, R. M., and Dmitrovsky, E. Reverse transcription polymerase chain reaction for the rearranged retinoic acid receptor- clarifies diagnosis and detects minimal residual disease in acute promyelocytic leukemia. Proc. Natl. Acad. Sci. USA, 89: , Castaigne, S., Balitrand, N., de Thé, H., Déjean, A., Degos, L., and Chomienne, C. A PML/retinoic acid receptor- fusion transcript is constantly detected by RNA-based polymerase chain reaction in acute promyelocytic leukemia. Blood, 79: , Borrow, J., Goddard, A. D., Gibbons, B., Kapz, F., Swirsky, D., Fioretos, T., Dube, I., Winfield, D. A., Kingston, J., Hagemeijer, A., Rees, J. K., Lister, T. A., and Solomon, E. Diagnosis of acute promyelocytic leukaemia by RT-PCR: detection of PML-RARA and RAR- PML fusion transcripts. Br. J. Haematol., 82: , LoCoco, F., Diverio, D., Pandolfi, P. P., Biondi, A., Rossi, V., Avvisati, G., Rambaldi, A., Arcese, W., Petti, M. C., and Meloni, G. Molecular evaluation of residual disease as a predictor of relapse in acute promyelocytic leukemia. Lancet, 340: , Miller, W. H., Jr., Levine, K., DeBlasio, A., Frankel, S. R., Dmitrovsky, E., and Warrell, R. P., Jr. Detection of minimal residual disease in acute promyelocytic leukemia by a reverse transcription polymerase chain reaction assay for the PML/RAR- fusion mrna. Blood, 82: , Huang, W., Sun, G-L., Li, Z-S., Cao, Q., Lu, Y., Jang, G-S., Zhang, F-Q., Chai, J-R., Wang, Z-Y., Waxman, S., Chen, Z., and Chen, S-J. Acute promyelocytic leukemia: clinical relevance of two major PML- RAR isoforms and detection of minimal residual disease by retrotranscriptase/polymerase chain reaction to predict relapse. Blood, 82: , Jurcic, J. G., Miller, W. H., Jr., DeBlasio, A., Scheinberg, D. A., and Warrell, R. P., Jr. Prognostic significance of minimal residual disease detection and PML/RAR- isoform type: long-term follow-up in acute promyelocytic leukemia. Blood, 88: 635a, Diverio, D., Rossi, V., Avvisati, G., DeSantis, S., Pistilli, A., Pane, F., Saglio, G., Martinelli, G., Petti, M. C., Santoro, A., Pelicci, P. G., Mandelli, F., Biondi, A., and LoCoco, F., for the GIMEMA and AIEOP Cooperative Groups. Early detection of relapse by prospective reverse transcription-polymerase chain reaction analysis of the PML/RAR fusion gene in patients with acute promyelocytic leukemia enrolled in the GIMEMA-AIEOP multicenter AIDA trial. Blood, 92: , Huang, M. E., Ye, Y. C., Chen, S. R., Chai, J. R., Lu, J. X., Lin, Z., Zhoa, L., Gu, L. J., and Wang, Z-Y. Use of all-trans retinoic acid in the treatment of acute promyelocytic leukemia. Blood, 72: , Castaigne, S., Chomienne, C., Daniel, M. T., Berger, R., Fenaux, P., and Degos, L. All-trans retinoic acid as a differentiation therapy for acute promyelocytic leukemia. I. Clinical results. Blood, 76: , Warrell, R. P., Jr., Frankel, S. R., Miller, W. H., Jr., Scheinberg, D. A., Itri, L. M., Hittelman, W. N., Vyas, R., Andreeff, M., Tafuri, A., Jakubowski, A., Gabrilove, J., Gordon, M. S., and Dmitrovsky, E. Differentiation therapy of acute promyelocytic leukemia with tretinoin (all-trans-retinoic acid). N. Engl. J. Med., 324: , Benedetti, F., Tong, W., Jurcic, J., Gill, G., Truglia, J., and Warrell, R. P., Jr. Clinical activity and pharmacology of 9-cis retinoic acid in patients with acute promyelocytic leukemia. Proc. Am. Soc. Clin. Oncol., 15: 359, Scheinberg, D. A., Tanimoto, M., McKenzie, S., Strife, A., Old, L. J., and Clarkson, B. D. Monoclonal antibody M195: a diagnostic marker for acute myelogenous leukemia. Leukemia (Baltimore), 3: , Sabbath, K. D., Ball, E. D., Larcom, P., Davis, R. B., and Griffin, J. D. Heterogeneity of clonogenic cells in acute myeloblastic leukemia. J. Clin. Investig., 75: , Griffin, J. D., Linch, D., Sabbath, K. D., Larcom, P., and Schlossman, S. F. A monoclonal antibody reactive with normal and leukemic human myeloid progenitor cells. Leuk. Res., 8: , Andrews, R. G., Takahashi, M., Segal, G. M., Powell, J. S., Bernstein, I. D., and Singer, J. W. The L4F3 antigen is expressed by unipotent colony-forming cells but not by their precursors. Blood, 68: , Scheinberg, D. A., Lovett, D., Divgi, C. R., Graham, M. C., Berman, E., Pentlow, K., Feirt, N., Finn, R. D., Clarkson, B. D., Gee, T. S., Larson, S. M., Oettgen, H. F., and Old, L. J. A Phase I trial of monoclonal antibody M195 in acute myelogenous leukemia: specific bone marrow targeting and internalization of radionuclide. J. Clin. Oncol., 9: , Schwartz, M. A., Lovett, D. R., Redner, A., Finn, R. D., Graham, M. C., Divgi, C. R., Dantis, L., Gee, T. S., Andreeff, M., Old, L. J., Larson, S. M., and Scheinberg, D. A. Dose-escalation trial of M195 labeled with iodine 131 for cytoreduction and marrow ablation in relapsed or refractory myeloid leukemias. J. Clin. Oncol., 11: , Jurcic, J. G., Caron, P. C., Nikula, T. K., Papadopoulos, E. B., Finn, R. D., Gansow, O. A., Miller, W. H., Jr., Geerlings, M. W., Warrell, R. P., Jr., Larson, S. M., and Scheinberg, D. A. Radiolabeled anti-cd33 monoclonal antibody M195 for myeloid leukemias. Cancer Res., 55 (Suppl.): 5908s 5910s, Jurcic, J. G., Caron, P. C., Miller, W. H., Jr., Yao, T. J., Maslak, P., Finn, R. D., Larson, S. M., Warrell, R. P., Jr., and Scheinberg, D. A. Sequential targeted therapy for relapsed acute promyelocytic leukemia with all-trans retinoic acid and anti-cd33 monoclonal antibody M195. Leukemia (Baltimore), 9: , Co, M. S., Avdalovic, N. M., Caron, P. C., Avdalovic, M. V., Scheinberg, D. A., and Queen, C. Chimeric and humanized antibodies with specificity for the CD33 antigen. J. Immunol., 148: , Caron, P. C., Co, M. S., Bull, M. K., Avdalovic, N. M., Queen, C., and Scheinberg, D. A. Biological and immunological features of humanized M195 (anti-cd33) monoclonal antibodies. Cancer Res., 52: , Caron, P. C., Jurcic, J. G., Scott, A. M., Finn, R. D., Divgi, C. R., Graham, M. C., Jureidini, I. M., Sgouros, G., Tyson, D., Old, L. J., Larson, S. M., and Scheinberg, D. A. A Phase IB trial of humanized antibody M195 (anti-cd33) in myeloid leukemia: specific targeting without immunogenicity. Blood, 83: , Caron, P. C., Dumont, L., and Scheinberg, D. A. Supersaturating infusional humanized anti-cd33 monoclonal antibody HuM195 in myelogenous leukemia. Clin. Cancer Res., 4: , Cheson, B. D., Cassileth, P. A., Head, D. R., Schiffer, C. A., Bennett, J. M., Bloomfield, C. D., Brunning, R., Gale, R. P., Grever, M. R., and Keating, M. J. Report of the National Cancer Institutesponsored workshop on definitions of diagnosis and response in acute myeloid leukemia. J. Clin. Oncol., 8: , LoBuglio, A. F., Wheeler, R. H., Trang, J., Haynes, A., Rogers, K., Harvey, E. B., Sun, L., Ghrayeb, J., and Khazaeli, M. B. Mouse/human chimeric monoclonal antibody in man: kinetics and immune response. Proc. Natl. Acad. Sci. USA, 86: , Frankel, S. R., Eardley, A., Heller, G., Berman, E., Miller, W. H., Jr., Dmitrovsky, E., and Warrell, R. P., Jr. All-trans retinoic acid for acute promyelocytic leukemia. Ann. Intern. Med., 120: , 1994.

9 380 Molecular Remission with HuM195 in APL 32. Soignet, S., Fleischauer, A., Polyak, T., Heller, G., and Warrell, R. P., Jr. All-trans retinoic acid significantly increases five year survival in patients with acute promyelocytic leukemia: long-term follow-up of the New York study. Cancer Chemother. Pharmacol., 40 (Suppl): S25 S29, Diverio, D., Pandolfi, P. P., Biondi, A., Avvisati, G., Petti, M. C., Mandelli, F., Pelicci, P. G., and LoCoco, F. Absence of reverse transcription-polymerase chain reaction detectable residual disease in patients with acute promyelocytic leukemia in long-term remission. Blood, 82: , Mandelli, F., Diverio, D., Avvisati, G., Luciano, A., Barbui, T., Bernasconi, C., Broccia, G., Cerri, R., Falda, M., Fioritoni, G., Leoni, F., Liso, V., Petti, M. C., Rodeghiero, F., Saglio, G., Vegna, M. L., Visani, G., Jehn, U., Willemze, R., Muus, P., Pelicci, P. G., Biondi, A., and LoCoco, F., for the GIMEMA and AIEOP Cooperative Groups. Molecular remission in PML/RAR -positive acute promyelocytic leukemia by combined all-trans retinoic acid and idarubicin (AIDA) therapy. Blood, 90: , Fenaux, P., Castaigne, S., Dombret, H., Archimbaud, E., Cuarte, M., Morel, P., Lamy, T., Tilly, H., Guerci, A., Maloisel, F., Bordessoiule, D., Sadoun, A., Tiberghien, P., Fegueux, N., Daniel, M. T., Chomienne, C., and Degos, L. All-trans retinoic acid followed by intensive chemotherapy gives a high complete remission rate and may prolong remissions in newly diagnosed acute promyelocytic leukemia: a pilot study on 26 cases. Blood, 80: , Fenaux, P., LeDeley, M. C., Castaigne, S., Archibaud, E., Chomienne, C., Link, H., Guerci, A., Duarte, M., Daniel, M. T., Bowen, D., Huebner, G., Bauters, F., Fegueux, N., Fey, M., Sanz, B., Lowenberg, B., Maloisel, F., Auzanneau, G., Sadoun, G., Gardin, C., Bastion, Y., Ganser, A., Jacky, E., Dombret, H., Chastang, C., and Degos, L., for the European APL 91 Group. Effect of all-trans-retinoic acid in newly diagnosed acute promyelocytic leukemia. Results of a multicenter randomized trial. Blood, 82: , Tallman, M. S., Andersen, J. W., Schiffer, C. A., Appelbaum, F. R., Feusner, J. H., Ogden, A., Shepherd, L., Willman, C., Bloomfield, C. D., Rowe, J. M., and Wiernik, P. H. All-trans retinoic acid in acute promyelocytic leukemia. N. Engl. J. Med., 337: , Fenaux, P., Chastang, C., Chevret, S., Sanz, M., Dombert, H., Archimbaud, E., Fey, M., Rayon, C., Huguet, F., Sotto, J-J., Gardin, C., Makhoul, P. C., Travade, P., Solary, E., Fegueux, N., Bordessoule, D., San Miguel, J., Link, H., Desablens, B., Stamatoullas, A., Deconinck, E., Maloisel, F., Castaigne, S., Preudhomme, C., and Degos, L., for the European APL Group. A randomized comparison of all-trans-retinoic acid (ATRA) followed by chemotherapy and ATRA plus chemotherapy and the role of maintenance therapy in newly diagnosed acute promyelocytic leukemia. Blood, 94: , Weisburg, J. H., Roepe, P. D., Dzekunov, S., and Scheinberg, D. A. Intracellular ph and multidrug resistance regulate complement-mediated cytotoxicity of nucleated human cells. J. Biol. Chem., 274: , Soignet, S. L., Maslak, P., Wang, Z-G., Jhanwar, S., Calleja, E., Dardashti, L. J., Corso, D., DeBlasio, A., Gabrilove, J., Scheinberg, D. A., Pandolfi, P. P., and Warrell, R. P., Jr. Complete remission after treatment of acute promyelocytic leukemia with arsenic trioxide. N. Engl. J. Med., 339: , 1998.

10 Molecular Remission Induction with Retinoic Acid and Anti-CD33 Monoclonal Antibody HuM195 in Acute Promyelocytic Leukemia Joseph G. Jurcic, Tony DeBlasio, Larry Dumont, et al. Clin Cancer Res 2000;6: Updated version Access the most recent version of this article at: Cited articles Citing articles This article cites 36 articles, 24 of which you can access for free at: This article has been cited by 20 HighWire-hosted articles. Access the articles at: alerts Sign up to receive free -alerts related to this article or journal. Reprints and Subscriptions Permissions To order reprints of this article or to subscribe to the journal, contact the AACR Publications Department at To request permission to re-use all or part of this article, use this link Click on "Request Permissions" which will take you to the Copyright Clearance Center's (CCC) Rightslink site.