Anti-Mycotoxin Additives
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1 Anti-Mycotoxin Additives
2 EFFICACY OF MYCOAD AGAINST AFLATOXIN IN LAYERS EXPERIMENTAL PROTOCOL Caged ISA Brown layers already in production were transferred from a commercial farm to an experimental unit at Samitec in Santa María, RS, Brazil. A seven-day adaptation period to the new facilities was allowed before starting the trial at 40 weeks of age. Birds were distributed using a complete randomized design into 3 treatments with 4 replicates of 10 layers each kept in communitarian cages (1.40 m²/cage) with slatted floor, equipped with semi-automatic feeders and automatic nipple drinkers. The building was maintained at an environmental temperature of 20 C ± 1 C and all laying hens were kept under uniform management conditions. All ingredients were tested free of mycotoxins contamination. The aflatoxin (AFL) used for the contaminated diets was produced in LAMIC (Lab. of Mycotoxicological Analysis). At the beginning of the trial feed consumption was kept at 120 g/bird and later increased based on egg production, following the genetic management guide. Water was supplied ad libitum and the experimental diets fed for 41 days. Performance parameters and egg characteristics were evaluated weekly. The relative weight of the liver was evaluated at the end of the trial AFL 3. AFL+MYCOAD Aflatoxin MYCOAD * 0.25 * 0.25 = 2.5 kg MYCOAD/MT RESULTS Performance, egg quality and relative liver weight after 41 days of treatment Production Mass kg Weight g Feed Conversion kg/kg Meat and blood spots Liver Weight Mortality a 1.88 a 61.3 a 2.61 a 0.60 c 2.31 c 0 AFL b 1.28 b 59.8 c 3.76 b 0.75 a 2.97 a 5.0 AFL 0.25 MYCOAD a 1.80 a 60.5 b 2.70 a 0.69 b 2.67 b 2.5 a, b, c Values within each column with different letters are significantly different (P< 0.05)
3 Aflatoxin Aflatoxin + MYCOAD Production kg 1.8 Mass g Weight kg : kg 4 Feed Conversion Meat and blood spots a 0.69 b 0.60 c 0.5 AFL AFL+MYCOAD a, b, c Values with different letters are significantly different (P< 0.05)
4 arget rgan P rotection Livers of 46 week-old hens after 41 days of treatment Aflatoxin Aflatoxin + MYCOAD 2.31 c 2.97 a 2.67 b Beneficial effects of MYCOAD production Mass Feed Conversion Relative Liver Weight Meat and Blood Spots Aflatoxin Aflatoxin MYCOAD CONCLUSIONS The addition of 0.25 of MYCOAD to a layer diet contaminated with aflatoxin significantly improved egg production, egg mass and feed conversion with a significant decrease in the liver size and the presence of meat and blood spots in the eggs. MYCOAD demonstrated its efficacy in preventing the toxic effects caused by aflatoxin in laying hens.
5 EFFICACY OF MYCOAD + MYCOAD AZ AGAINST AFLATOXIN + FUMONISIN + T-2 TOXIN IN LAYERS EXPERIMENTAL PROTOCOL Exactly the same protocol described in the previous trial was used in this experiment. MYCOAD and MYCOAD AZ were both added to the feed as anti-mycotoxin additives. A combination of mycotoxins, including aflatoxin, fumonisin (FUM) and T-2 toxin (T-2) were simultaneously added to the contaminated diets. All mycotoxins were produced by LAMIC. Aflatoxin Fumonisin T-2 Toxin Mycoad + Mycoad AZ AFL+FUM+T-2 10 ppm 1 ppm 3. AFL+FUM+T-2 MYCOAD+MYCOAD AZ 10 ppm 1 ppm * * 0.25 = 2.5 kg MYCOAD/MT 0.10 = 1.0 kg MYCOAD AZ/MT RESULTS Performance, egg quality and relative liver weight after 41 days of treatment Production Mass kg Weight g Meat and Blood spots Feed Conversion kg/kg Liver Weight Mortality AFL 10 ppm FUM 1 ppm T-2 AFL+FUM+T-2 MYCOAD 0.25 MYCOAD AZ a 1.88 a 61.3 a 0.60 c 2.61 a 2.31 c c 1.17 c 60.2 b 0.79 a 3.87 c 3.25 a b 1.54 b 59.8 b 0.68 b 3.01 b 2.90 b 2.5 a, b, c Values within each column with different letters are significantly different (P< 0.05)
6 AFL + FUM + T-2 AFL + FUM + T-2 + MYCOAD + MYCOAD AZ 85 Production kg 1.8 Mass g Weight kg : kg 4 Feed Conversion Meat and blood spots a b 0.60 c 0.5 AFL+FUM+T2 AFL+FUM+T2 +MYCOAD+AZ a, b, c Values with different letters are significantly different (P< 0.05)
7 arget rgan P rotection Livers of 46 week-old hens after 41 days of treatment AFL+ FUM+T-2 AFL+FUM+T-2 +MYCOAD+MYCOAD AZ 2.31 c 3.25 a 2.90 b Beneficial effects of MYCOAD + MYCOAD AZ production Mass Feed Conversion Relative Liver Weight Meat and Blood Spots AFL+FUM+T AFL+FUM+T-2 +MYCOAD+MYCOAD AZ CONCLUSIONS The combination of 0.25 of MYCOAD MYCOAD AZ added to the diet contaminated with aflatoxin, fumonisin, and T-2 toxin, significantly improved egg production, egg mass and feed conversion with a significant decrease in the liver size and the presence of meat and blood spots in the eggs. MYCOAD AZ combined with MYCOAD, demonstrated its efficacy in preventing the toxic effects caused by the combination of aflatoxin + fumonisin + T-2 toxin in laying hens. Reference. Mallmann, C. A., D. Sturza, L. Giacomini, M. Contreras, and D. Zaviezo. Evaluation of two anti-mycotoxin additives in commercial layers. Instituto Samitec Lamic UFSM, Brazil. Special Nutrients, USA (for publication)
8 Does your Anti-Mycotoxin Additive meet the basic TOP & FACTS? MYCOTOXIN Aflatoxin Ochratoxin T-2 Toxin Fumonisin Zearalenone DON Target rgan Protection FACTS ORGAN Liver Kidney Oral lesion Heart / Lung / Liver Reproductive Liver In vivo dosage with TOP results 2.5 kg / MT 1 kg / MT Recommended commercial dosage MYCOAD 2.5 kg / MT 1 kg / MT The clay is always obtained from the same mine N/A N/A MYCOAD MYCOAD AZ NO NO * MYCOAD AZ Copyright January Special Nutrients. All Rights Reserved Approved in Texas, USA, against Aflatoxin N/A Approved in the European Union against Aflatoxin. Regulation #1831 / 2003 (1m 588) N/A ENDOTOXIN adsorption N/A Efficacy approved by LAMIC and other institutions against the following number of mycotoxins Efficacy approved by LAMIC and other institutions in different types of animals Nutrient adsorption 4 4 In vitro efficacy test every: 100 MT 18 MT 6 NO 5 NO * Test performed with 4 kg with 30,000 ppb of Fumonisin N/A= not applicable SPECIAL NUTRIENTS THE MYCOTOXINS SPECIALIST MYCOAD = Cobind, Toxfree Standard MYCOAD AZ= Cobind AZ, Toxfree
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