Talk list. Definition: open questions. Functions: manyfold. The road ahead: GMP, open questions. From discovery. To basic science

Size: px
Start display at page:

Download "Talk list. Definition: open questions. Functions: manyfold. The road ahead: GMP, open questions. From discovery. To basic science"

Transcription

1 Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles* * and from the NIH exrna Communication Consortium Ciro Tetta MD

2 Talk list Definition: open questions From discovery Functions: manyfold To basic science The road ahead: GMP, open questions To clinical application Yanez-Mo, M et al, Biological activities of EVs and their physiological functions, JEV 2015; 4: Quesenberry PJ et al. Potential fucntional applications of EVs: a report by the NIHCommon Fund Extracellular RNA Communication Consortium. JEV 2015; 4:27575

3 Documents Documents Documents EV research has been of interest for over a decade, but with major growth beginning in Extracellular vesicle total 4,058 documents Microvesicle total 501 documents 600 Exosome total 3,249 documents Scopus search and data analysis (Search terms in Abstract, Title, Keywords, from , all document types, all subject areas ) 3

4 DEFINITION 4

5 Extracellular vesicles Exosomes Cell-derived Vesicles Microvesicles Apoptotic bodies Camussi G. et al. Kidney Int 2010; Tetta C. et al. Endocrine,

6 Size Distribution of Extracellular Vesicles György B, et al. Cell Mol Life Sci 2011;68:

7 Nothing goes lost! From Byproduct to Product Open questions: what is the golden standard technique/s? What do we mean by that?

8 FUNCTION 8

9 The biogenesis and uptake of exrna. Exocytosis is commonly mediated via clathrin-coated pits after which endocytic vesicles progress from early to late endosomes also referred to as multivesicular bodies. ExRNA can be detected in the exosomes or in the microvesicles associated to proteins or as part of lipoprotein particles, particularly HDL. Open questions: how exrnas are released for functional activity upon entry into recipient cells Patton JG, JEV

10 EVs from stem /progenitor cells as a paracrine / endocrine mechanism EVs may mediate a horizontal transfer of genetic information Murine Embryonic Stem Cells - Ratajczak J et al. Leukemia Yuan A et al. PLoS One 2009 Adult Stem Cells - EVs - human EPC-derived EVs: proangiogenesis on endothelial cells - Deregibus et al. Blood human EPC-derived EV microrna: - Cantaluppi et al. Cell Transplant 2012; Kidney Int human MSC-derived EV mrna - Bruno et al. JASN human MSC-derived EV microrna - Collino et al. PloS One human liver SC-derived EV mrna - Herrera et al. J Cell Mol Med. 2010

11 Pro-angiogenetic and repair effect

12 Characterization of MSC-derived MVs MVs shuttle selected patterns of mrna and microrna (GEO d.b. accession GSE12243) A total of 365 transcripts were found*, 132 were associated to Entrez Gene identifiers by IPA 6.0 analysis, including: -mrna characteristic of mesenchymal cell lineages, such as neural, osteogenic, epithelial and hematopoietic; -mrna involved in the control of transcription, cell proliferation and immune regulation. * 42 were co-expressed in MV and cells Collino et al. PLoS One 2010

13 Collino et al. PLoS One 2010 Expression of RNA-binding proteins in MVs RNPs in MSC (stress granules) RNPs in MVs Western blot analysis TEM of MVs Immunogold analysis MV as compartimentalization site for RNA

14 Characterization of mirnas Precursor mirnas Mature mirnas mrna degradation by mirnas Collino et al. PLoS One 2010

15 EVIDENCE FOR MV-MEDIATED TRANSFER OF GENETIC INFORMATION A proof of transfer in target cells of mrna delivered from MVs derives from: -experiments showing the transfer of reporter genes. MVs derived from human stem cells may deliver both in vitro and in vivo human mrna -Bruno et al. JASN 2009 Herrera et al. J Cell Mol. Med experiments showing translation into protein of reporter mrna such as the green fluorescence protein mrna (GFP). Cells targeted with MVs carrying GFP mrna transiently produced the GFP proteins Deregibus et al. Blood 2007; Aliotta et al. 2010

16 EVIDENCE FOR MV-MEDIATED TRANSFER OF GENETIC INFORMATION MV-mediated transfer of specific mirnas to target cells (mtec) Selective expression of mirnas (in a-amanitin-treated mtec) Internalization of PKH26-labeled MVs in mtec (by fluorescence-labeled reporter mirnas) Internalization of PKH26-labeled MVs in mtec Reduction of expression of target proteins of specific mirnas PTEN mir-21 cyclin D1 mir-100, mir-99,mir-223 Bcl-2 mir-34, mir-181b, mir-16 Collino et al PLoS One 2010

17 EVs Protect Against Acute Tubular Injury

18 EVs Protect from Lethal Cisplatin-induced Renal Injury Effect of EVs on in vitro expression of gene involved in apoptosis in tubular cells treated with cisplatin: CIS +MV vs CIS Up-regulated Down-regulated BCL2 BCL2L1(BCL-XL) BIRC8 LTA CASP8 CASP1 BCL2L11 TP73 HRK CARD6

19 Bidirectional exchange of information between injured cells and stem cells EV-mediated reprogramming of renal resident cells by transfer of specific mrnas, mirnas and proteins -Deregibus et al. Blood Bruno et al. J Am Soc Nephrol Gatti et al. NDT 2011 & Proteins -Dooner et al. Stem Cells Dev Aliotta et al. Exp Hematology Quesenberry et al. Stem Cells Dev Open questions: how exrnas are released for functional activity upon entry into recipient cells? Are the epigenetic effects really all due to exrna transfer or only partly?

20 PEOPLE MARIE CURIE ACTIONS Marie Curie Industry-Academia Partnerships and Pathways (IAPP) Call: FP7-PEOPLE-2013-IAPP Role of Extracellular Vesicles from Stem Cells in organ injury (EVStemInjury) IAPP2013 EVStemInjury Project Catenion meeting Mar 2014

21 The partners PUBLIC SECTOR PRIVATE SECTOR

22 Potential clinical utility of EVs Considerations for regulatory requirements GMP Protocol of isolation to be defined (optimal cell source culturing, isolation, characterization markers, cost-effective production / automation, optimal dosing), Size range for EVs Biochemical markers (for EVs broadly and for specific preparations) Purity of EV preparation and absence of undesired components Sterility of cell growth and EV isolation environments Optimal EV isolation methods (including for scalability, purity, and particular EV subsets) Development of EV stabilization methods Optimal storage conditions for EV stability Analytical methods and standards for all of the above Serum-free media use (likely to be compulsory)

23 Potential clinical utility of EVs Considerations for regulatory requirements Consistency The preparative method results in a heterogeneous population. Further separation to a homogenous population would not be technically possible because there are no definite size, density or protein markers that can reliably define a specific population. EV product should not have an absolute requirement for characterization of cell-surface markers, content, or for homogeneity but rather should be based on the definition and description of a size-defined, functionally effective population isolated using a robust, reproducible and validated process.

24 Potential clinical utility of EVs Considerations for regulatory requirements Safety - The safety profile needs to be demonstrated in preclinical studies. Efficacy - Functional potency test relevant to the proposed application. Dose and delivery - This would depend on efficacy and safety profiles, bioavailability and specific formulation of the therapeutic product e.g. the EV product may be cryopreserved, lyophilized, used in suspension, or combined into a matrix. 24

25 GMP Production: what do we need - Isolation Protocols for large recovery: - UC / SEC / FFF / Polymeric P - FAQ: HOW DO THEY AFFECT THE BIOLOGICAL ACTIVITIES OF EV? - Criteria for EV characterization: reproducible functional tests (forget about homogeneity!) - FAQ: WHICH ARE THE CRITERIA? - Validated potency assays designed for different app - The assays qualified for potenecy should be neither too sophisticated, time-consuming nor too expensive. A point that remains to be solved is quantitation of EVs 25

26 Translational Centre of Regenerative Medicine University of Torino M C. Tetta V. Fonsato F. Collino M.. Herrera V.M. Navarro F. Figliolini M. Tapparo C. Cavallari M.C. Deregibus F. Antico C. Contursi G. Camussi S. Bruno C. Grange V. Cantaluppi C. Pasquino M. Delena A. Iavello A.Carpanetto A.Pitino S.Previdi Cell-Factory: M.Gunetti Administrative Office G. Ferri

27 27

Therapeutic potential of mesenchymal stem cell-derived microvesicles

Therapeutic potential of mesenchymal stem cell-derived microvesicles Nephrol Dial Transplant (2012) 27: 3037 3042 doi: 10.1093/ndt/gfs168 Therapeutic potential of mesenchymal stem cell-derived microvesicles Luigi Biancone 1, Stefania Bruno 1, Maria Chiara Deregibus 1, Ciro

More information

Mesenchymal stem cells release exosomes that transfer mirnas to endothelial cells and promote angiogenesis

Mesenchymal stem cells release exosomes that transfer mirnas to endothelial cells and promote angiogenesis Mesenchymal stem cells release exosomes that transfer mirnas to endothelial cells and promote angiogenesis Tanja Wagner 1 Introduction 2 Mesenchymal stem cells (MSCs) non-haematopoietic, multipotent stem

More information

Stem cell extracellular vesicles and kidney injury

Stem cell extracellular vesicles and kidney injury Review Article Page 1 of 10 Stem cell extracellular vesicles and kidney injury Cristina Grange 1, Corinne Iampietro 2, Benedetta Bussolati 2 1 Department of Medical Sciences, 2 Department of Biotechnology

More information

Utility of Circulating micrornas in Cardiovascular Disease

Utility of Circulating micrornas in Cardiovascular Disease Utility of Circulating micrornas in Cardiovascular Disease Pil-Ki Min, MD, PhD Cardiology Division, Gangnam Severance Hospital, Yonsei University College of Medicine Introduction Biology of micrornas Circulating

More information

Journal Club Semmler Lorenz

Journal Club Semmler Lorenz Beer et al. 2015 - Analysis of the Secretome of Apoptotic Peripheral Blood Mononuclear Cells: Impact of Released Proteins and Exosomes for Tissue Regeneration Journal Club 13.11.2017 1 Introduction to

More information

Long term stability of isolated human serum derived exosomes

Long term stability of isolated human serum derived exosomes Long term stability of isolated human serum derived exosomes Candice de Boer (PhD student) Regenerative Medicine Laboratory Supervisor: Associate Professor Neil Davies Exosomes First discovered during

More information

MicroRNAs: a new source of biomarkers in radiation response. Simone Moertl, Helmholtz Centre Munich

MicroRNAs: a new source of biomarkers in radiation response. Simone Moertl, Helmholtz Centre Munich MicroRNAs: a new source of biomarkers in radiation response Simone Moertl, Helmholtz Centre Munich The RNA World mrna rrna coding snorna trna scarna RNA snrna noncoding lincrna rasirna RNAi anti-sense

More information

Service and Collaboration

Service and Collaboration Summary Section 7 Introduction 68 Exosome and Microvesicle Isolation 69 EV protein quantification, sceening and profiling 69 Nanoparticle Tracking Analysis (NTA) 70 EV Nucleic Acid isolation 70 Nucleic

More information

Intracellular Vesicular Traffic Chapter 13, Alberts et al.

Intracellular Vesicular Traffic Chapter 13, Alberts et al. Intracellular Vesicular Traffic Chapter 13, Alberts et al. The endocytic and biosynthetic-secretory pathways The intracellular compartments of the eucaryotic ell involved in the biosynthetic-secretory

More information

Role of extracellular RNA-carrying vesicles in cell differentiation and reprogramming

Role of extracellular RNA-carrying vesicles in cell differentiation and reprogramming Quesenberry et al. Stem Cell Research & Therapy (2015) 6:153 DOI 10.1186/s13287-015-0150-x REVIEW Role of extracellular RNA-carrying vesicles in cell differentiation and reprogramming Peter J. Quesenberry

More information

Production of Exosomes in a Hollow Fiber Bioreactor

Production of Exosomes in a Hollow Fiber Bioreactor Production of Exosomes in a Hollow Fiber Bioreactor John J S Cadwell, President and CEO, FiberCell Systems Inc INTRODUCTION Exosomes are small lipid membrane vesicles (80-120 nm) of endocytic origin generated

More information

micro-rnas as biomarkers in children who underwent surgery for CHD

micro-rnas as biomarkers in children who underwent surgery for CHD micro-rnas as biomarkers in children who underwent surgery for CHD mentors: Dr. Yael Nevo-Caspi & Prof. Gidi Paret Or Bercovich Liat Mor What will we talk about Congenital heart defects (CHD) Scientific

More information

USE OF EXOSOMES IN TRANSLATIONAL AND CLINICAL RESEARCH. Eva Colás Ortega Postdoc researcher IRBLleida

USE OF EXOSOMES IN TRANSLATIONAL AND CLINICAL RESEARCH. Eva Colás Ortega Postdoc researcher IRBLleida USE OF EXOSOMES IN TRANSLATIONAL AND CLINICAL RESEARCH Eva Colás Ortega Postdoc researcher IRBLleida ecolas@irblleida.cat THERE ARE DIFFERENT TYPE OF VESICLES RELEASED BY CELLS MICROVESICLES Size: 100-1000nm

More information

RENAL FUNCTION BIOMARKERS

RENAL FUNCTION BIOMARKERS HERNÁN TRIMARCHI HOSPITAL BRITÁNICO DE BUENOS AIRES ARGENTINA 2015 1 DISCLOSURES Served as a consultant and/or has received lecture honoraria from: ALEXION BRISTOL MYERS SQUIBB GENZYME NOVARTIS PFIZER

More information

Cancer Problems in Indonesia

Cancer Problems in Indonesia mirna and Cancer : mirna as a Key Regulator in Cancer Sofia Mubarika 2 nd Symposium Biomolecular Update in Cancer PERABOI Padang 18 Mei 2013 Cancer Problems in Indonesia 1. Chemoresistency / recurrency

More information

Profiles of gene expression & diagnosis/prognosis of cancer. MCs in Advanced Genetics Ainoa Planas Riverola

Profiles of gene expression & diagnosis/prognosis of cancer. MCs in Advanced Genetics Ainoa Planas Riverola Profiles of gene expression & diagnosis/prognosis of cancer MCs in Advanced Genetics Ainoa Planas Riverola Gene expression profiles Gene expression profiling Used in molecular biology, it measures the

More information

TISSUE-SPECIFIC STEM CELLS

TISSUE-SPECIFIC STEM CELLS TISSUE-SPECIFIC STEM CELLS Human Liver Stem Cell-Derived Microvesicles Inhibit Hepatoma Growth in SCID Mice by Delivering Antitumor MicroRNAs VALENTINA FONSATO, a FEDERICA COLLINO, a MARIA BEATRIZ HERRERA,

More information

Chapter 13: Vesicular Traffic

Chapter 13: Vesicular Traffic Chapter 13: Vesicular Traffic Know the terminology: ER, Golgi, vesicle, clathrin, COP-I, COP-II, BiP, glycosylation, KDEL, microtubule, SNAREs, dynamin, mannose-6-phosphate, M6P receptor, endocytosis,

More information

MagCapture Exosome Isolation Kit PS Q&A

MagCapture Exosome Isolation Kit PS Q&A MagCapture Exosome Isolation Kit PS Q&A Specifications and performance P.1 Comparison of the conventional method P.2 Operation methods and composition P.4 Amount of starting sample P.5 Analysis after exosomes

More information

Insight into cancer research from discovery to validation

Insight into cancer research from discovery to validation Insight into cancer research from discovery to validation 1 Exosomes in cancer research: from exosome isolation to biomarker discovery Zheng Songyue, PhD Technical Sales Specialists Life Technologies The

More information

THESIS. ROMANIAN ACADEMY Institute of Cellular Biology and Pathology Nicolae Simionescu, Bucharest

THESIS. ROMANIAN ACADEMY Institute of Cellular Biology and Pathology Nicolae Simionescu, Bucharest ROMANIAN ACADEMY Institute of Cellular Biology and Pathology Nicolae Simionescu, Bucharest THESIS Study of progenitor cells differentiation into cardiomyocytes in order to improve cardiac cellular transplant

More information

ORIGINAL ARTICLE OPEN

ORIGINAL ARTICLE OPEN International Journal of Obesity (2016) 40, 102 111 2016 Macmillan Publishers Limited All rights reserved 0307-0565/16 www.nature.com/ijo OPEN ORIGINAL ARTICLE Obesity reduces the pro-angiogenic potential

More information

Exosomes/microvesicles as a mechanism of cell-to-cell communication

Exosomes/microvesicles as a mechanism of cell-to-cell communication http://www.kidney-international.org & 2010 International Society of Nephrology Exosomes/microvesicles as a mechanism of cell-to-cell communication Giovanni Camussi 1, Maria C. Deregibus 1, Stefania Bruno

More information

The clinical relevance of circulating, cell-free and exosomal micrornas as biomarkers for gynecological tumors

The clinical relevance of circulating, cell-free and exosomal micrornas as biomarkers for gynecological tumors Department of Tumor Biology The clinical relevance of circulating, cell-free and exosomal micrornas as biomarkers for gynecological tumors cfdna Copenhagen April 6-7, 2017 Heidi Schwarzenbach, PhD Tumor

More information

A new approach to characterize exosomes and study their interactions with biomolecules. Silvia Picciolini PhD student 30 th May 2016

A new approach to characterize exosomes and study their interactions with biomolecules. Silvia Picciolini PhD student 30 th May 2016 A new approach to characterize exosomes and study their interactions with biomolecules Silvia Picciolini PhD student 30 th May 2016 Extracellular vesicles (EVs) Most body cells release physiologically

More information

Extracellular Vesicle Quantification Kits

Extracellular Vesicle Quantification Kits Summary Section 3 Introduction 34 ExoTEST TM Ready to Use ELISA quantification kit 35 Exo-FACS Ready to Use kit 38 Ordering information Exosome research is a relatively novel and exciting field of biomedical

More information

Olfactory ensheathing glia

Olfactory ensheathing glia Olfactory ensheathing glia From Wikipedia, the free encyclopedia Neuroglia of the brain shown by Golgi's method. Olfactory ensheathing glia (OEG), also known as olfactory ensheathing cells (OECs) or olfactory

More information

The Detection of Exosomes Released from Mesenchymal Stem Cells. and Their Therapeutic Potential in Multiple Sclerosis

The Detection of Exosomes Released from Mesenchymal Stem Cells. and Their Therapeutic Potential in Multiple Sclerosis The Detection of Exosomes Released from Mesenchymal Stem Cells and Their Therapeutic Potential in Multiple Sclerosis Shivani Dave Briarcliff High School Introduction: Cognitive impairments and general

More information

MicroRNA expression profiling and functional analysis in prostate cancer. Marco Folini s.c. Ricerca Traslazionale DOSL

MicroRNA expression profiling and functional analysis in prostate cancer. Marco Folini s.c. Ricerca Traslazionale DOSL MicroRNA expression profiling and functional analysis in prostate cancer Marco Folini s.c. Ricerca Traslazionale DOSL What are micrornas? For almost three decades, the alteration of protein-coding genes

More information

AKI Recovery Induced by Mesenchymal Stromal Cell- Derived Extracellular Vesicles Carrying MicroRNAs

AKI Recovery Induced by Mesenchymal Stromal Cell- Derived Extracellular Vesicles Carrying MicroRNAs AKI Recovery Induced by Mesenchymal Stromal Cell- Derived Extracellular Vesicles Carrying MicroRNAs Federica Collino,* Stefania Bruno, Danny Incarnato, Daniela Dettori, Francesco Neri, Paolo Provero, Margherita

More information

MicroRNA in Cancer Karen Dybkær 2013

MicroRNA in Cancer Karen Dybkær 2013 MicroRNA in Cancer Karen Dybkær RNA Ribonucleic acid Types -Coding: messenger RNA (mrna) coding for proteins -Non-coding regulating protein formation Ribosomal RNA (rrna) Transfer RNA (trna) Small nuclear

More information

Influence of erythropoietin on microvesicles derived from mesenchymal stem cells protecting renal function of chronic kidney disease

Influence of erythropoietin on microvesicles derived from mesenchymal stem cells protecting renal function of chronic kidney disease Wang et al. Stem Cell Research & Therapy (2015) 6:100 DOI 10.1186/s13287-015-0095-0 RESEARCH Open Access Influence of erythropoietin on microvesicles derived from mesenchymal stem cells protecting renal

More information

mirna Biomarkers Seena K. Ajit PhD Pharmacology & Physiology Drexel University College of Medicine October 12, 2017

mirna Biomarkers Seena K. Ajit PhD Pharmacology & Physiology Drexel University College of Medicine October 12, 2017 mirna Biomarkers Seena K. Ajit PhD Pharmacology & Physiology Drexel University College of Medicine October 12, 2017 Outline Introduction Circulating mirnas as potential biomarkers for pain Complex regional

More information

Cell therapy: enhancing the therapeutic potential of cardiac progenitors for delivery post myocardial infarction. Rita Alonaizan

Cell therapy: enhancing the therapeutic potential of cardiac progenitors for delivery post myocardial infarction. Rita Alonaizan Cell therapy: enhancing the therapeutic potential of cardiac progenitors for delivery post myocardial infarction Rita Alonaizan Department of Physiology, Anatomy & Genetics St Catherine s College Supervisor:

More information

The Angio-Ready Assay System

The Angio-Ready Assay System The Angio-Ready Assay System Kevin Grady, B.S. Product Line Business Manager, ATCC Cell Systems Charles Zou, Ph.D. Senior Scientist, ATCC Cell Systems About ATCC Founded in 1925, ATCC is a non-profit organization

More information

CD34 + VEGFR-3 + progenitor cells have a potential to differentiate towards lymphatic endothelial cells

CD34 + VEGFR-3 + progenitor cells have a potential to differentiate towards lymphatic endothelial cells CD34 + VEGFR-3 + progenitor cells have a potential to differentiate towards lymphatic endothelial cells Tan YZ et al. J Cell Mol Med. (2014 Mar;18(3):422-33) Denise Traxler-Weidenauer April 2014 Introduction

More information

Milk micro-rna information and lactation

Milk micro-rna information and lactation Christophe Lefèvre, BioDeakin,. Deakin University, Geelong VIC Australia Milk micro-rna information and lactation New Signals in milk? - Markers of milk and lactation status? - Signal infant development?

More information

Regenerative Orthopedics

Regenerative Orthopedics Regenerative Orthopedics WHERE ARE WE NOW? W. SCOTT WAUGH, MD, CAQSM, RMSK NONOPORTHO.COM W. Scott Waugh, MD, RMSK Edmond, OK Integrative Medical Solutions and Nonop Ortho Baylor University Waco, TX University

More information

A phase I pilot study of safety and feasibility of stem cell therapy for AIDS lymphoma using stem cells treated with a lentivirus vector encoding

A phase I pilot study of safety and feasibility of stem cell therapy for AIDS lymphoma using stem cells treated with a lentivirus vector encoding A phase I pilot study of safety and feasibility of stem cell therapy for AIDS lymphoma using stem cells treated with a lentivirus vector encoding multiple anti-hiv RNAs John A. Zaia, M.D. John J. Rossi,

More information

BL-8040: BEST-IN-CLASS CXCR4 ANTAGONIST FOR TREATMENT OF ONCOLOGICAL MALIGNANCIES. Overview and Mechanism of Action Dr.

BL-8040: BEST-IN-CLASS CXCR4 ANTAGONIST FOR TREATMENT OF ONCOLOGICAL MALIGNANCIES. Overview and Mechanism of Action Dr. BL-8040: BEST-IN-CLASS CXCR4 ANTAGONIST FOR TREATMENT OF ONCOLOGICAL MALIGNANCIES Overview and Mechanism of Action Dr. Leah Klapper, CSO 88 BL-8040: Novel CXCR4 Antagonist For Hematological Cancers Indications:

More information

Meeting Report. From December 8 to 11, 2012 at Atlanta, GA, U.S.A

Meeting Report. From December 8 to 11, 2012 at Atlanta, GA, U.S.A Meeting Report Affiliation Department of Transfusion Medicine and Cell Therapy Name Hisayuki Yao Name of the meeting Period and venue Type of your presentation Title of your presentation The 54 th Annual

More information

Extracellular Vesicle RNA isolation Kits

Extracellular Vesicle RNA isolation Kits Extracellular Vesicle RNA isolation Kits Summary Section 4 Introduction 42 Exo-TotalRNA and TumorExo-TotalRNA isolation kits 43 Extracellular Vesicle RNA extraction kits Ordering information Products can

More information

Introduction. Keywords Extracellular vesicles. Mesenchymal stem cells. Exosomes. Microvesicles. Acute kidney injury. Kidney regeneration

Introduction. Keywords Extracellular vesicles. Mesenchymal stem cells. Exosomes. Microvesicles. Acute kidney injury. Kidney regeneration Stem Cell Rev and Rep (2017) 13:226 243 DOI 10.1007/s12015-016-9713-1 Exosome and Microvesicle-Enriched Fractions Isolated from Mesenchymal Stem Cells by Gradient Separation Showed Different Molecular

More information

Cell implantation after myocardial infarction: a 10 years experience from the ICREC laboratory

Cell implantation after myocardial infarction: a 10 years experience from the ICREC laboratory Cell implantation after myocardial infarction: a 10 years experience from the ICREC laboratory BANFF-SCT Joint Scientific Meeting 2017 Barcelona, 29 th March Santi Roura, PhD Grup ICREC IGTP HuGTiP (Badalona)

More information

Regulation of the IGF axis by TGF-b during periosteal chondrogenesis: implications for articular cartilage repair

Regulation of the IGF axis by TGF-b during periosteal chondrogenesis: implications for articular cartilage repair Regulation of the IGF axis by TGF-b during periosteal chondrogenesis: implications for articular cartilage repair Chapter 04 Boek 1_Gie.indb 55 21-05-2007 12:27:33 Chapter 04 Abstract Goal: TGF-b and IGF-I

More information

Nasser Aghdami MD., PhD

Nasser Aghdami MD., PhD CONTRIBUTON OF HUMAN INDUCED PLURIPOTENT STEM CELL DERIVED ENDOTHELIAL CELLS IN VASCULAR REGENERATION OF BLEOMYCIN-INDUCED SCLERODERMA MOUSE MODEL Nasser Aghdami MD., PhD Department of Regenerative Medicine

More information

kidney disease VISHAL S. VAIDYA, PH.D. ASSISTANT PROFESSOR OF MEDICINE & ENVIRONMENTAL HEALTH Harvard School of Public Health

kidney disease VISHAL S. VAIDYA, PH.D. ASSISTANT PROFESSOR OF MEDICINE & ENVIRONMENTAL HEALTH Harvard School of Public Health µrnas: New frontiers in kidney disease VISHAL S. VAIDYA, PH.D. ASSISTANT PROFESSOR OF MEDICINE & ENVIRONMENTAL HEALTH Harvard Medical School Brigham and Women s Hospital Harvard School of Public Health

More information

Stem Cell Primer. Hyun Bae, M.D. Professor of Surgery Department of Surgery Director of Education Cedars Spine Center

Stem Cell Primer. Hyun Bae, M.D. Professor of Surgery Department of Surgery Director of Education Cedars Spine Center Stem Cell Primer Hyun Bae, M.D. Professor of Surgery Department of Surgery Director of Education Cedars Spine Center Perinatal Stem Cells Perinatal Stem Cells are defined as stem cells that are associated

More information

SCI Annual Report (2017) Editorial Office

SCI Annual Report (2017) Editorial Office SCI Annual Report (2017) Editorial Office editor@sci-online.org Outline of the Report 1. Basic Information 2. Editors of SCI 3. Distribution of Editorial Board Members 4. Number of Published Articles (results

More information

Application potential of stem/progenitor cell-derived extracellular vesicles in renal diseases

Application potential of stem/progenitor cell-derived extracellular vesicles in renal diseases Sun et al. Stem Cell Research & Therapy (2019) 10:8 https://doi.org/10.1186/s13287-018-1097-5 REVIEW Application potential of stem/progenitor cell-derived extracellular vesicles in renal diseases Xiao

More information

Cellular Physiology and Biochemistry

Cellular Physiology and Biochemistry Original Paper 2015 The Author(s). 2015 Published The Author(s) by S. Karger AG, Basel Published online: November 27, 2015 www.karger.com/cpb Published by S. Karger AG, Basel 2194 1421-9778/15/0376-2194$39.50/0

More information

Review and Public RAC Discussion of Protocol #

Review and Public RAC Discussion of Protocol # Review and Public RAC Discussion of Protocol #0508 725 A phase I pilot study of safety and feasibility of stem cell therapy for AIDS lymphoma using stem cells treated with a lentivirus vector encoding

More information

Reprogramming through micrornas Stefanie Dimmeler

Reprogramming through micrornas Stefanie Dimmeler Klinikum der Johann Wolfgang Goethe Universität Frankfurt am Main Reprogramming through micrornas Stefanie Dimmeler Conflict of interest: T2cure GmbH, Miragen Non-coding DNA & RNA and micrornas Human Genome

More information

mirna Dr. S Hosseini-Asl

mirna Dr. S Hosseini-Asl mirna Dr. S Hosseini-Asl 1 2 MicroRNAs (mirnas) are small noncoding RNAs which enhance the cleavage or translational repression of specific mrna with recognition site(s) in the 3 - untranslated region

More information

Exosomes Derived from Mesenchymal Stem Cells Suppress Angiogenesis by Down-Regulating VEGF Expression in Breast Cancer Cells

Exosomes Derived from Mesenchymal Stem Cells Suppress Angiogenesis by Down-Regulating VEGF Expression in Breast Cancer Cells Exosomes Derived from Mesenchymal Stem Cells Suppress Angiogenesis by Down-Regulating VEGF Expression in Breast Cancer Cells Jong-Kuen Lee 1, Sae-Ra Park 1, Bong-Kwang Jung 1, Yoon-Kyung Jeon 1,2, Yeong-Shin

More information

Emerging Concepts of Tumor Exosome Mediated Cell Cell Communication

Emerging Concepts of Tumor Exosome Mediated Cell Cell Communication Emerging Concepts of Tumor Exosome Mediated Cell Cell Communication Huang-Ge Zhang Editor Emerging Concepts of Tumor Exosome Mediated Cell Cell Communication 2123 Editor Huang-Ge Zhang University of Louisville

More information

Epigenetic Principles and Mechanisms Underlying Nervous System Function in Health and Disease Mark F. Mehler MD, FAAN

Epigenetic Principles and Mechanisms Underlying Nervous System Function in Health and Disease Mark F. Mehler MD, FAAN Epigenetic Principles and Mechanisms Underlying Nervous System Function in Health and Disease Mark F. Mehler MD, FAAN Institute for Brain Disorders and Neural Regeneration F.M. Kirby Program in Neural

More information

MicroRNA dysregulation in cancer. Systems Plant Microbiology Hyun-Hee Lee

MicroRNA dysregulation in cancer. Systems Plant Microbiology Hyun-Hee Lee MicroRNA dysregulation in cancer Systems Plant Microbiology Hyun-Hee Lee Contents 1 What is MicroRNA? 2 mirna dysregulation in cancer 3 Summary What is MicroRNA? What is MicroRNA? MicroRNAs (mirnas) -

More information

microrna Presented for: Presented by: Date:

microrna Presented for: Presented by: Date: microrna Presented for: Presented by: Date: 2 micrornas Non protein coding, endogenous RNAs of 21-22nt length Evolutionarily conserved Regulate gene expression by binding complementary regions at 3 regions

More information

Human induced pluripotent stem cell derived cardiomyocytes are a more relevant model for assessing drug-induced effects on mitochondrial function

Human induced pluripotent stem cell derived cardiomyocytes are a more relevant model for assessing drug-induced effects on mitochondrial function Human induced pluripotent stem cell derived cardiomyocytes are a more relevant model for assessing drug-induced effects on mitochondrial function than H9C2 cells 16 January 2013 SLAS2013 Presentation Outline

More information

Exosomes, Extracellular Vesicles A Technology Review

Exosomes, Extracellular Vesicles A Technology Review Exosomes, Extracellular Vesicles A Technology Review 1 Author, Data Collection Date, Notes The Author Gary M. Oosta, holds a Ph.D. in Biophysics from Massachusetts Institute of Technology and a B.A. in

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION DOI:.38/ncb3399 a b c d FSP DAPI 5mm mm 5mm 5mm e Correspond to melanoma in-situ Figure a DCT FSP- f MITF mm mm MlanaA melanoma in-situ DCT 5mm FSP- mm mm mm mm mm g melanoma in-situ MITF MlanaA mm mm

More information

Mesenchymal stem cell-derived extracellular vesicles: novel frontiers in regenerative medicine

Mesenchymal stem cell-derived extracellular vesicles: novel frontiers in regenerative medicine Keshtkar et al. Stem Cell Research & Therapy (2018) 9:63 https://doi.org/10.1186/s13287-018-0791-7 REVIEW Mesenchymal stem cell-derived extracellular vesicles: novel frontiers in regenerative medicine

More information

ALOPECIA AREATA RESEARCH SUMMIT Forging the Future

ALOPECIA AREATA RESEARCH SUMMIT Forging the Future ALOPECIA AREATA RESEARCH SUMMIT Forging the Future PRELIMINARY PROGRAM Tuesday, December 4 Wednesday, December 5, 2018 Lerner Hall, Columbia University, New York City, USA Summit Co-Chairs Jerry Shapiro,

More information

BIOACTIVE RENAL CELLS AUGMENT KIDNEY FUNCTION IN A RODENT MODEL OF CHRONIC KIDNEY DISEASE

BIOACTIVE RENAL CELLS AUGMENT KIDNEY FUNCTION IN A RODENT MODEL OF CHRONIC KIDNEY DISEASE BIOACTIVE RENAL CELLS AUGMENT KIDNEY FUNCTION IN A RODENT MODEL OF CHRONIC KIDNEY DISEASE Rusty Kelley, PhD Regenerative Medicine & Biology Tengion Labs May 24, 2010 ISCT Annual Meeting, Philadelphia PA

More information

Extracellular vesicles and ceramide: new mediators for macrophage chemotaxis? Natalie J. Torok

Extracellular vesicles and ceramide: new mediators for macrophage chemotaxis? Natalie J. Torok Extracellular vesicles and ceramide: new mediators for macrophage chemotaxis? Natalie J. Torok Department of Medicine, Gastroenterology and Hepatology, UC Davis, Sacramento, CA, and Northern California

More information

CD34+ Cells: A Comparison of Stem and Progenitor Cells in Cord Blood, Peripheral Blood, and the Bone Marrow

CD34+ Cells: A Comparison of Stem and Progenitor Cells in Cord Blood, Peripheral Blood, and the Bone Marrow White Paper September 2016 CD34+ Cells: A Comparison of Stem and Progenitor Cells in Cord Blood, Peripheral Blood, and the Bone Marrow Lily C. Trajman, PhD Introduction: Hematopoietic Stem Cells (HSCs)

More information

Paternal exposure and effects on microrna and mrna expression in developing embryo. Department of Chemical and Radiation Nur Duale

Paternal exposure and effects on microrna and mrna expression in developing embryo. Department of Chemical and Radiation Nur Duale Paternal exposure and effects on microrna and mrna expression in developing embryo Department of Chemical and Radiation Nur Duale Our research question Can paternal preconceptional exposure to environmental

More information

Bhatnagar et al, 2010 Cell Death and Disease Manuscript # CDDIS T

Bhatnagar et al, 2010 Cell Death and Disease Manuscript # CDDIS T Bhatnagar et al, Cell Death and Disease Manuscript # CDDIS--98-T Supplemental Materials. Supplemental Figure Legends Supplemental Figure (A) WPE-NA and WPE-NB6 cells were treated with 4 nm of Docetaxel

More information

Post-transcriptional regulation of an intronic microrna

Post-transcriptional regulation of an intronic microrna Post-transcriptional regulation of an intronic microrna Carl Novina Dana-Farber Cancer Institute Harvard Medical School Broad Institute of Harvard and MIT Qiagen Webinar 05-17-11 Outline 1. The biology

More information

Prokaryotes and eukaryotes alter gene expression in response to their changing environment

Prokaryotes and eukaryotes alter gene expression in response to their changing environment Chapter 18 Prokaryotes and eukaryotes alter gene expression in response to their changing environment In multicellular eukaryotes, gene expression regulates development and is responsible for differences

More information

Antioxidants And Stem Cells For Coronary Heart Disease By Philip B Oliva

Antioxidants And Stem Cells For Coronary Heart Disease By Philip B Oliva Antioxidants And Stem Cells For Coronary Heart Disease By Philip B Oliva If you are searched for the ebook by Philip B Oliva Antioxidants and Stem Cells for Coronary Heart Disease in pdf form, in that

More information

Type of file: PDF Size of file: 0 KB Title of file for HTML: Supplementary Information Description: Supplementary Figures

Type of file: PDF Size of file: 0 KB Title of file for HTML: Supplementary Information Description: Supplementary Figures Type of file: PDF Size of file: 0 KB Title of file for HTML: Supplementary Information Description: Supplementary Figures Supplementary Figure 1 mir-128-3p is highly expressed in chemoresistant, metastatic

More information

Human breast milk mirna, maternal probiotic supplementation and atopic dermatitis in offsrping

Human breast milk mirna, maternal probiotic supplementation and atopic dermatitis in offsrping Human breast milk mirna, maternal probiotic supplementation and atopic dermatitis in offsrping Melanie Rae Simpson PhD candidate Department of Public Health and General Practice Norwegian University of

More information

Capricor Therapeutics

Capricor Therapeutics Therapeutics Conference Call to Discuss the HOPE-2 Clinical Trial NASDAQ: CAPR November 29, 2017 Forward-Looking Statements Statements in this presentation regarding the efficacy, safety, and intended

More information

Cancer as a disease of development; Developmental therapies: Anti- Angiogenesis; Stem cells and tissue regeneration.

Cancer as a disease of development; Developmental therapies: Anti- Angiogenesis; Stem cells and tissue regeneration. Cancer as a disease of development; Developmental therapies: Anti- Angiogenesis; Stem cells and tissue regeneration Mitesh Shrestha What is Cancer? Unrestricted cell growth: tumor cell population 1x10^9

More information

Oncolytic Adenovirus Complexes Coated with Lipids and Calcium Phosphate for Cancer Gene Therapy

Oncolytic Adenovirus Complexes Coated with Lipids and Calcium Phosphate for Cancer Gene Therapy Oncolytic Adenovirus Complexes Coated with Lipids and Calcium Phosphate for Cancer Gene Therapy Jianhua Chen, Pei Gao, Sujing Yuan, Rongxin Li, Aimin Ni, Liang Chu, Li Ding, Ying Sun, Xin-Yuan Liu, Yourong

More information

X P. Supplementary Figure 1. Nature Medicine: doi: /nm Nilotinib LSK LT-HSC. Cytoplasm. Cytoplasm. Nucleus. Nucleus

X P. Supplementary Figure 1. Nature Medicine: doi: /nm Nilotinib LSK LT-HSC. Cytoplasm. Cytoplasm. Nucleus. Nucleus a b c Supplementary Figure 1 c-kit-apc-eflu780 Lin-FITC Flt3-Linc-Kit-APC-eflu780 LSK Sca-1-PE-Cy7 d e f CD48-APC LT-HSC CD150-PerCP-cy5.5 g h i j Cytoplasm RCC1 X Exp 5 mir 126 SPRED1 SPRED1 RAN P SPRED1

More information

stem cell products Basement Membrane Matrix Products Rat Mesenchymal Stem Cell Growth and Differentiation Products

stem cell products Basement Membrane Matrix Products Rat Mesenchymal Stem Cell Growth and Differentiation Products stem cell products Basement Membrane Matrix Products Rat Mesenchymal Stem Cell Growth and Differentiation Products Stem Cell Qualified Extracellular Matrix Proteins Stem cell research requires the finest

More information

The roadmap towards the industrial and clinical applications of extracellular vesicles

The roadmap towards the industrial and clinical applications of extracellular vesicles The roadmap towards the industrial and clinical applications of extracellular vesicles Exosomes and EVs have come a long way: major scientific milestones timeline Means to frame the size and evolution

More information

Comparison of open chromatin regions between dentate granule cells and other tissues and neural cell types.

Comparison of open chromatin regions between dentate granule cells and other tissues and neural cell types. Supplementary Figure 1 Comparison of open chromatin regions between dentate granule cells and other tissues and neural cell types. (a) Pearson correlation heatmap among open chromatin profiles of different

More information

endomembrane system internal membranes origins transport of proteins chapter 15 endomembrane system

endomembrane system internal membranes origins transport of proteins chapter 15 endomembrane system endo system chapter 15 internal s endo system functions as a coordinated unit divide cytoplasm into distinct compartments controls exocytosis and endocytosis movement of molecules which cannot pass through

More information

Asterias Biotherapeutics NYSE American: AST

Asterias Biotherapeutics NYSE American: AST Clinical-Stage Cell Therapy Programs Addressing Significant Unmet Medical Needs in Neurology and Oncology Asterias Biotherapeutics NYSE American: AST November 2017 Forward-Looking Statements Statements

More information

Exosomes secreted by Human Cardiac Progenitors contain mirna with cardioprotective and proangiogenic activities

Exosomes secreted by Human Cardiac Progenitors contain mirna with cardioprotective and proangiogenic activities Exosomes secreted by Human Cardiac Progenitors contain mirna with cardioprotective and proangiogenic activities Elisabetta Cervio, PhD Molecular Cardiology Laboratory Cardiocentro Ticino, Lugano, CH International

More information

Assaying micrornas in biofluids for detection of drug induced cardiac injury. HESI Annual Meeting State of the Science Session June 8, 2011

Assaying micrornas in biofluids for detection of drug induced cardiac injury. HESI Annual Meeting State of the Science Session June 8, 2011 Assaying micrornas in biofluids for detection of drug induced cardiac injury HESI Annual Meeting State of the Science Session June 8, 2011 Karol Thompson, PhD Center for Drug Evaluation & Research US Food

More information

Discovery and development of regenerative medicine products comprised of autologous cells and biomaterials. ISCT September 28, 2010 San Francisco, CA

Discovery and development of regenerative medicine products comprised of autologous cells and biomaterials. ISCT September 28, 2010 San Francisco, CA Discovery and development of regenerative medicine products comprised of autologous cells and biomaterials ISCT September 28, 2010 San Francisco, CA 1 Tengion s products catalyze regeneration INPUTS OUTPUTS

More information

Figure 1: Effects of cisplatin on survival of lung cancer cells.

Figure 1: Effects of cisplatin on survival of lung cancer cells. Figure 1 Figure 1: Effects of cisplatin on survival of lung cancer cells. To determine the IC 50 concentration of cisplatin, cells were treated with various concentrations of cisplatin and cell survival

More information

ADC Clinical Core Leaders meeting: Can we do more with biomarkers? Joseph Quinn, MD. April 16, 2016

ADC Clinical Core Leaders meeting: Can we do more with biomarkers? Joseph Quinn, MD. April 16, 2016 ADC Clinical Core Leaders meeting: Can we do more with biomarkers? Joseph Quinn, MD April 16, 2016 1 New initiatives with biomarkers of neurodegenerative disease: Extracellular micrornas as biomarkers

More information

Int J Clin Exp Pathol 2018;11(11): /ISSN: /IJCEP Dan Zhou 1,2, Wei Zhai 3, Miaomiao Zhang 1

Int J Clin Exp Pathol 2018;11(11): /ISSN: /IJCEP Dan Zhou 1,2, Wei Zhai 3, Miaomiao Zhang 1 Int J Clin Exp Pathol 2018;11(11):5157-5170 www.ijcep.com /ISSN:1936-2625/IJCEP0085323 Original Article Mesenchymal stem cell-derived extracellular vesicles promote apoptosis in RSC96 Schwann cells through

More information

Chapter 2. Investigation into mir-346 Regulation of the nachr α5 Subunit

Chapter 2. Investigation into mir-346 Regulation of the nachr α5 Subunit 15 Chapter 2 Investigation into mir-346 Regulation of the nachr α5 Subunit MicroRNA s (mirnas) are small (< 25 base pairs), single stranded, non-coding RNAs that regulate gene expression at the post transcriptional

More information

Exosomes/tricalcium phosphate combination scaffolds can enhance bone regeneration by activating the PI3K/Akt signalling pathway

Exosomes/tricalcium phosphate combination scaffolds can enhance bone regeneration by activating the PI3K/Akt signalling pathway Exosomes/tricalcium phosphate combination scaffolds can enhance bone regeneration by activating the PI3K/Akt signalling pathway Jieyuan Zhang, Xiaolin Liu, Haiyan Li, Chunyuan Chen, Bin Hu, Xin Niu, Qing

More information

2/26/2016. Types of Stem Cells: Agenda. Objectives

2/26/2016. Types of Stem Cells: Agenda. Objectives PAMET, March 5, 2016 Umbilical Cord Blood for Public Banking and Research Regenerative Medicine: Curing disease by repairing or replacing organs and systems through cellular and tissue engineering Bladder

More information

Cardiovascular. Diseases. a simple and standardized kit qpcr analysis of circulating platelet-derived micrornas

Cardiovascular. Diseases. a simple and standardized kit qpcr analysis of circulating platelet-derived micrornas microrna Biomarkers of Platelet Function Neurodegenerative Diseases Cardiovascular Diseases Musculoskeletal Diseases a simple and standardized kit qpcr analysis of circulating platelet-derived micrornas

More information

MicroRNA and Male Infertility: A Potential for Diagnosis

MicroRNA and Male Infertility: A Potential for Diagnosis Review Article MicroRNA and Male Infertility: A Potential for Diagnosis * Abstract MicroRNAs (mirnas) are small non-coding single stranded RNA molecules that are physiologically produced in eukaryotic

More information

Arthritis Research UK Submission to House of Lords Committee on Science and Technology Inquiry: Regenerative Medicine

Arthritis Research UK Submission to House of Lords Committee on Science and Technology Inquiry: Regenerative Medicine September 2012 Arthritis Research UK Submission to House of Lords Committee on Science and Technology Inquiry: Regenerative Medicine 1. Arthritis Research UK welcomes the opportunity to respond to the

More information

PERSPECTIVES. Stem Cell-Derived Extracellular Vesicles as a Novel Potential Therapeutic Tool for Tissue Repair

PERSPECTIVES. Stem Cell-Derived Extracellular Vesicles as a Novel Potential Therapeutic Tool for Tissue Repair PERSPECTIVES Stem Cell-Derived Extracellular Vesicles as a Novel Potential Therapeutic Tool for Tissue Repair BI CHEN, a,b QING LI, a BIZENG ZHAO, b YANG WANG a a Institute of Microsurgery on Extremities,

More information

Oncolytic Virotherapy: Targeting Cancer Stem Cells

Oncolytic Virotherapy: Targeting Cancer Stem Cells Oncolytic Virotherapy: Targeting Cancer Stem Cells Cancer Stem Cells (CSCs) or Cancer Initiating Cells (CICs) A consensus of five defining criteria has been established to affirm the existence of CICs:

More information

Supplementary fig. 1. Crystals induce necroptosis does not involve caspases, TNF receptor or NLRP3. A. Mouse tubular epithelial cells were pretreated

Supplementary fig. 1. Crystals induce necroptosis does not involve caspases, TNF receptor or NLRP3. A. Mouse tubular epithelial cells were pretreated Supplementary fig. 1. Crystals induce necroptosis does not involve caspases, TNF receptor or NLRP3. A. Mouse tubular epithelial cells were pretreated with zvad-fmk (10µM) and exposed to calcium oxalate

More information

PhD THESIS Epigenetic mechanisms involved in stem cell differentiation

PhD THESIS Epigenetic mechanisms involved in stem cell differentiation Romanian Academy Institute of Cellular Biology and Pathology "Nicolae Simionescu" PhD THESIS Epigenetic mechanisms involved in stem cell differentiation Coordinator: Acad. Maya Simionescu PhD Student:

More information

CRISPR-mediated Editing of Hematopoietic Stem Cells for the Treatment of β-hemoglobinopathies

CRISPR-mediated Editing of Hematopoietic Stem Cells for the Treatment of β-hemoglobinopathies CRISPR-mediated Editing of Hematopoietic Stem Cells for the Treatment of β-hemoglobinopathies Jennifer Gori American Society of Gene & Cell Therapy May 11, 2017 editasmedicine.com 1 Highlights Developed

More information