Product Datasheet. CD161/NK1.1 Antibody (PK136) NB Unit Size: 0.5 mg. Store at 4C. Do not freeze. Publications: 2

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1 Product Datasheet CD161/NK1.1 Antibody (PK136) NB Unit Size: 0.5 mg Store at 4C. Do not freeze. Publications: 2 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 12/10/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB CD161/NK1.1 Antibody (PK136) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity 0.5 mg 1.0 mg/ml Store at 4C. Do not freeze. Monoclonal PK % Sodium Azide IgG2a Kappa Protein A or G purified Buffer PBS (ph 7.2) Page 1 of 4 v.20.1 Updated 12/10/2018 Product Description Host Mouse Gene ID Gene Symbol Species Reactivity Notes Specificity/Sensitivity Immunogen Product Application Details Applications KLRB1 Mouse Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Mouse Monoclonal to NK-1.1 (PK136) NK-1+ cells from mouse spleen and bone marrow Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry- Paraffin, Immunoprecipitation Recommended Dilutions Flow Cytometry < 1.0 ug/10^6 cells, Immunohistochemistry 1:20-1:1000, Immunocytochemistry/Immunofluorescence 1:20-1:1000, Immunoprecipitation 1:20-1:1000, Immunohistochemistry-Paraffin 1:20-1:1000, Immunohistochemistry-Frozen 1:20-1:1000 Application Notes Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application. Immunohistochemistry- Paraffin was reported in scientific literature.

3 Images Analysis using the PE/Cy7 conjugate of NB Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC and staining buffer (autofluorescence) (left) or Anti-Mouse NK1.1 PE- Cy7 (right). Total viable cells were used for analysis. Page 2 of 4 v.20.1 Updated 12/10/2018 Mouse splenocytes were stained with CD49b (DX5) APC and purified NK1.1 (clone PK136) (top) or mouse IgG2a isotype control (bottom), followed by anti-mouse IgG2a FITC. C57BL/6 mouse splenocytes stained with purified PK136, followed by anti-mouse IgG2a FITC Mouse.

4 Staining of C57BL/6 mouse splenocytes with Anti-Mouse NK-1.1 PE and Anti-Mouse CD49b (Integrin alpha 2) FITC. Total viable cells were used for analysis. Page 3 of 4 v.20.1 Updated 12/10/2018 Analysis using the PerCP/Cy5.5 conjugate of NB Staining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC and 0.25 ug of Mouse IgG2a K Isotype Control PerCP-Cy5.5 (left) or 0.25 ug of Anti-Mouse NK1.1 PerCP-Cy5.5 (right). Total viable cells were used for analysis. Publications Hu B, Elinav E, Huber S et al. Inflammation-induced tumorigenesis in the colon is regulated by caspase-1 and NLRC4. Proc Natl Acad Sci U S A 2010 Dec 14 [PMID: ] (IHC-P, Mouse) Kanwar JR, Shen WP, Kanwar RK et al. Effects of survivin antagonists on growth of established tumors and B7-1 immunogene therapy. J Natl Cancer Inst. 93(20): Oct 17. [PMID: ]

5 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF007 NB720-B NBP NB PCP Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] Mouse IgG2a Isotype Control (M2AK) CD161/NK1.1 Antibody (PK136) [PerCP] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: