SUPPLEMENTAL TESTING. Tan Thean Yen
|
|
- Colleen Terry
- 5 years ago
- Views:
Transcription
1 SUPPLEMETAL TESTG Tan Thean Yen
2 To Supplement Definition: add as a supplement to what seems insufficient "supplement your diet"
3 Why supplement? urrent methods don t work well Additional information provided by supplemental testing Alternative approaches to current methods More rapid testing results
4 Why OT supplement? urrent breakpoints are adequate More work onfusing Slower testing results
5 Three areas to cover 1. inducible resistance v.s. de-repressed resistance 2. Beta-lactamases & beta-lactamase inhibitors 3. Heterogenous populations
6 Part One DUBLE V.S. DE-REPRESSED
7 D U T O nducible resistance
8 nducible resistance De-repressed or resistant D U T O May be OR Always
9 STAPHYLOO nducible resistance
10 lindamycin & Erythromycin Macrolide Erythromycin larithromycin Azithromycin Lincosamide lindamycin Lincomycin Streptogramin Quinupristin- Dalfopristin Pristinamycin
11 E R Y T H R O M Y Erythromycin lindamycin nterpretation S S Organism susceptible to both & L D A M Y R R R S Organism resistant to both May have inducible resistance
12 E R Y T H R O M Y & L D A M Y
13 E R Y T H R O M Y & L D A M Y nducible resistance
14 E R Y T H R O M Y Recommendations for MLS b lindamycin & erythromycin discs Staphylococci: 15-26mm apart Streptococci: 12 mm apart & L D A M Y look for flattening of zone of inhibition
15 E R Y T H R O M Y & L D A M Y Recommendations for MLS b broth dilution: use combination of erythromycin 4 µg/ml and clindamycin 0.5 µg/ml in a single well growth = inducible resistance OR use disc testing methods, on purity plate.
16 E R Y T H R O M Y & L D A M Y Other methods Agar dilution Sensitivity = 91%, specificity = 97% Vitek card Sensitivity = 91%, specificity = 100% Lavallee,., Rouleau, D., Gaudreau,., Roger, M., Tsimiklis,., Locas, M.., Gagnon, S., Delorme, J., Labbe, A.. (2010). Performance of an Agar Dilution Method and a Vitek 2 ard for Detection of nducible lindamycin Resistance in Staphylococcus spp.. J. lin. Microbiol. 48:
17 Part Two BETA-LATAMASES
18 Beta-lactamases LASS A: extended-spectrum beta-lactamases LASS : amp beta-lactamases LASS B: metallo-beta-lactamases
19 Plasmid (acquired) G E E A Q U S T O hromosomal (born with it)
20 amp A M P E Z Y M E S hromosomal Enterobacter spp. & most other Enterobacteriaceae Pseudomonas aeruginosa Plasmid-mediated Klebsiella spp. Salmonella spp. Proteus mirabilis (E. coli)
21 amp A M P E Z Y M E S chromosomal plasmid genes inducible de-repressed (always on) inducible de-repressed (always on)
22 AZ A M P MP E Z Y M E S
23 A M P E Z Y M E S nducible amp resistance
24 Extended spectrum betalactamases actually a family of related beta-lactamases SHV TEM E S B L three main groups: SHV TEM TX TX usually plasmid-borne
25 sulbactam Beta lactam Beta lactam inhibitor E S clavulanic acid B L tazobactam
26 ESBL & beta-lactamase inhibitors E S B L ESBL enzyme breaks down cephalosporin antibiotic
27 ESBL & beta-lactamase inhibitors E S B L nhibitor binds to ESBL enzyme. Prevents ESBL from breaking down antibiotic. antibiotic
28 ESBL & beta-lactamase inhibitors E Beta-lactamase inhibitors S B L compete with the beta-lactamase enzyme
29 ESBL & beta-lactamase inhibitors E S nhibitor > ESBL = susceptible ESBL > nhibitor = resistant B L
30 Double disk approximation E S B L lavulanic acid cephalosporin
31 efotaxime E S B L efotaxime & clavulanate
32 eftazidime + lavulanate M 0.25 E S B L eftazidime M > 32
33 Other beta-lactamases Beta-lactamase amp MBL KP nhibitor cloxacillin boronic acid EDTA mercaptopropionic acid (MPA) other chelating agents boronic acid
34 amp Substrate Enzyme nhibitor imipenem Strong inducer.. but generally not broken down by amp
35 amp Substrate Enzyme nhibitor imipenem strong inducer of amp AD broken down by amp cefoxitin amp cloxacillin
36 amp cefoxitin = small zone (disc) (by itself) high M (dilution) cefoxitin = larger zone (disc) & cloxacillin lower M (dilution)
37 Antibiotic 1 eftazidime eftazidime Meropenem 21 mm 21 mm 17 mm Antibiotic 2 eftazidime & clavulanate eftazidime & clavulanate Meropenem & EDTA 27 mm 16 mm 28 mm nterpretation
38 Part Three HETEROGEEOUS POPULATO
39 het er o ge ne ous Adjective Diverse in character or content: "a large and heterogeneous collection"
40 Heterogeneous mostly susceptible small number of resistant strains
41 STAPHYLOO difficult to detect resistance
42 Methicillin resistance M E T H L L mediated by the meca gene
43 Methicillin testing (disc) M E T H L L S. aureus& S. lugdunensis Oxacillin (for S. aureus only) efoxitin (for both) coagulase negative staph efoxitin only
44 Thymidine-dependent Small olonial Variant S. aureus Kahl B et al. J. lin. Microbiol. 2003;41:
45 MRSA and SV s M E T H L L slow-growing, atypical phenotype often seen in: cystic fibrosis, foreign-body infections &osteomyelitis susceptibility may be difficult to test best to use pbp2a or meca detection Frank Kipp, Karsten Becker, Georg Peters, and hristof von Eiff. Evaluation of Different Methods To Detect Methicillin Resistance in Small-olony Variants of Staphylococcus aureus. J lin Microbiol M arch; 42(3):
46 other methods M E T H L L detection of pbp2a latex agglutination kits immunochromatographic kits (Binax) detection of meca gene by hybridisation Evigene detection of meca gene by PR BD Diagnostics, epheid, Roche Molecular Diagnostics
47 Moderately resistant S. aureus M E T H L L don t have the meca gene altered pbp (penicillin binding proteins) rare phenotype cefoxitin (S), oxacillin (R) should respond to drugs like augmentin, cephalexin
48 Vancomycin V A O M Y large molecule, diffuses slowly in agar no disc diffusion criteria for S. aureus resistance to vancomycin: low-level (intermediate-resistance) high-level (vana mediated) heterogenous (spectrum)
49 V A O M Y hvsa Heterogenous resistance to vancomycin
50 hvsa V A O M Y GRD strip Etest macro method Population analysis
51 SUPPLEMETAL TESTG OR M?
52 Traditional way Tested result Reported result eftriaxone eftriaxone R efotaxime S efotaxime R eftazidime R eftazidime R efepime S efepime R key-hole effect present ESBL present
53 Proposed new way Tested result Reported result eftriaxone eftriaxone efotaxime S efotaxime S eftazidime R eftazidime R efepime S efepime S key-hole effect present ESBL present
54 Why?
55 For the gene The presence of a resistance gene makes a difference to whether or not a particular antibiotic will work. t s not just the M.
56 For the gene Look for particular resistance enzymes: amp ESBL MBL Modify the susceptibility according to the presence of resistance enzymes.
57 For the M... The breakpoint for each antibiotic determines whether patient will respond to that antibiotic. t doesn t matter what the resistance gene is.
58 FOR AGAST Life is much simpler! s it **really** true? Simple = more consistent lab results s it true for **every** enzyme?
59 Opinion! hange susceptibility if present inducible clindamycin resistance (blood & bone) Enterobacter spp. and cephalosporin susceptible (blood) Don t know plasmid amp ESBL KP MBL (probably don t change tested result)
60 onclusions Some resistant organisms may need supplemental methods to detect Susceptibility testing can be complicated Resistance mechanism or tested M? ot yet quite sure.
Laboratory CLSI M100-S18 update. Paul D. Fey, Ph.D. Associate Professor/Associate Director Josh Rowland, M.T. (ASCP) State Training Coordinator
Nebraska Public Health Laboratory 2008 CLSI M100-S18 update Paul D. Fey, Ph.D. Associate Professor/Associate Director Josh Rowland, M.T. (ASCP) State Training Coordinator Agenda Discuss 2008 M100- S18
More informationDiscussion points CLSI M100 S19 Update. #1 format of tables has changed. #2 non susceptible category
Discussion points 2009 CLSI M100 S19 Update Nebraska Public Health Laboratory Changes most important to routine antimicrobial susceptibility testing. Documents available Janet Hindler discussion slide
More informationScreening and detection of carbapenemases
Screening and detection of carbapenemases For many isolates with carbapenemases the MICs of carbapenems are around the susceptible breakpoint making resistance difficult to detect - particularly with automated
More informationAntibiotic Resistance Pattern of Blood and CSF Culture Isolates At NHLS Academic Laboratories (2005)
Antibiotic Resistance Pattern of Blood and CSF Culture Isolates At NHLS Academic Laboratories (2005) Streptococcus pneumoniae (SP) Blood Culture Isolates Penicillin intermediate Penicillin Cefotaxime 336
More informationPrevalence of Extended Spectrum -Lactamases In E.coli and Klebsiella spp. in a Tertiary Care Hospital
ISSN: 2319-7706 Volume 3 Number 10 (2014) pp. 474-478 http://www.ijcmas.com Original Research Article Prevalence of Extended Spectrum -Lactamases In E.coli and Klebsiella spp. in a Tertiary Care Hospital
More informationUpdate on CLSI and EUCAST
Update on CLSI and EUCAST 1 Completed work» Cephalosporin breakpoints for Enterobacteriaceae ESBL screens MIC versus resistance mechanism» Carbapenem breakpoints for Enterobacteriaceae Modified Hodge Test»
More informationPhenotypic detection of ESBLs and carbapenemases
Phenotypic detection of ESBLs and carbapenemases Standardized susceptibility testing residential workshop 2016 Katie Hopkins PhD Clinical Scientist Antimicrobial Resistance and Healthcare Associated Infections
More informationUniversity of Alberta Hospital Antibiogram for 2007 and 2008 Division of Medical Microbiology Department of Laboratory Medicine and Pathology
University of Alberta Hospital Antibiogram for 2007 and 2008 Division of Medical Microbiology Department of Laboratory Medicine and Pathology This material is supported in part by unrestricted educational
More informationReceived 31 January 2011/Returned for modification 2 March 2011/Accepted 15 March 2011
JOURNAL OF CLINICAL MICROBIOLOGY, May 2011, p. 1965 1969 Vol. 49, No. 5 0095-1137/11/$12.00 doi:10.1128/jcm.00203-11 Copyright 2011, American Society for Microbiology. All Rights Reserved. Comparative
More informationFrequency of Occurrence and Antimicrobial Susceptibility of Bacteria from ICU Patients with Pneumonia
Frequency of Occurrence and Antimicrobial Susceptibility of Bacteria from ICU Patients with Pneumonia Helio S. Sader, M.D.* Mariana Castanheira, Ph.D. Rodrigo E. Mendes, Ph.D. Robert K. Flamm, Ph.D. JMI
More informationDetecting carbapenemases in Enterobacteriaceae
Detecting carbapenemases in Enterobacteriaceae David Livermore Health Protection Agency, Colindale, London 12 August 2003 Mechanisms of carbapenem R in Enterobacteria Impermeability + AmpC or ESBL Metallo
More informationSep Oct Nov Dec Total
LB PAGE 2 LB PAGE 3 Sep Oct Nov Dec 2007 2007 2007 2007 Total Repeat Information Total Repeats 35 15 17 9 76 Repeat Rate 6.01% 0.17% 1.12% 0.39% 2.07% Repeat Chemistry 25 0 2 0 27 Repeat Extraction 1 0
More informationNONFERMENTING GRAM NEGATIVE RODS. April Abbott Deaconess Health System Evansville, IN
NONFERMENTING GRAM NEGATIVE RODS April Abbott Deaconess Health System Evansville, IN OBJECTIVES Discuss basic limitations to assessing carbapenem resistance in nonfermenting GNRs Discuss antimicrobial
More informationAffinity of Doripenem and Comparators to Penicillin-Binding Proteins in Escherichia coli and ACCEPTED
AAC Accepts, published online ahead of print on February 00 Antimicrob. Agents Chemother. doi:./aac.01-0 Copyright 00, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights
More informationHelen Heffernan and Rosemary Woodhouse Antibiotic Reference Laboratory, Institute of Environmental Science and Research Limited (ESR); July 2014.
Annual survey of extended-spectrum -lactamase (ESBL)-producing Enterobacteriaceae, 2013 Helen Heffernan and Rosemary Woodhouse Antibiotic Reference Laboratory, Institute of Environmental Science and Research
More informationAntimicrobial Activity and Spectrum of PPI-0903M (T-91825), a Novel Cephalosporin, Tested against a Worldwide Collection of Clinical Strains
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Aug. 2005, p. 3501 3512 Vol. 49, No. 8 0066-4804/05/$08.00 0 doi:10.1128/aac.49.8.3501 3512.2005 Copyright 2005, American Society for Microbiology. All Rights Reserved.
More informationβ-lactamase inhibitors
β-lactamase inhibitors Properties, microbiology & enzymology DAVID M LIVERMORE Professor of Medical Microbiology, UEA Lead on Antibiotic Resistance, Public Health England β-lactamase classes A B C D Serine
More informationJournal of Infectious Diseases and
Journal of Infectious Diseases & Therapy ISSN: 2332-0877 Journal of Infectious Diseases and Therapy Santanirand et al., J Infect Dis Ther 2018, 6:5 DOI: 10.4172/2332-0877.1000378 Research Article Open
More informationEUCAST Frequently Asked Questions. by author. Rafael Cantón Hospital Universitario Ramón y Cajal, Madrid, Spain EUCAST Clinical Data Coordinator
EUCAST Frequently Asked Questions Rafael Cantón Hospital Universitario Ramón y Cajal, Madrid, Spain EUCAST Clinical Data Coordinator Erika Matuschek EUCAST Development Laboratory, Växjö Sweden Monday 24
More informationSTRUCTURE OF COMMONLY USED PENICILLINS
PENICILLINS Alice Prince I. CHEMISTRY A basic structure of penicillins consists of a nucleus with three components: a thiazolidine ring, a β-lactam ring and a side chain. The side chain determines, in
More informationResults and experience of BARN ESBL project. Marina Ivanova and project team Tallinn
Results and experience of BARN ESBL project Marina Ivanova and project team Tallinn 13.05.2013 Aim of the project Improvement of detection and surveillance of resistance caused by extended spectrum betalactamase
More informationExpert rules. for Gram-negatives
Academic Perspective in Expert rules Emerging Issues of Resistance in Gram-ve Bacteria for Gram-negatives Trevor Winstanley Sheffield Teaching Hospitals Presented on behalf of David Livermore University
More informationPublic Health Surveillance for Multi Drug Resistant Organisms in Orange County
Public Health Surveillance for Multi Drug Resistant Organisms in Orange County Matt Zahn, MD Medical Director Epidemiology and Assessment Orange County Public Health Antimicrobial Mechanisms of Action
More informationDevelopment of C sporins. Beta-lactam antibiotics - Cephalosporins. Second generation C sporins. Targets - PBP s
Beta-lactam antibiotics - Cephalosporins Development of C sporins Targets - PBP s Activity - Cidal - growing organisms (like the penicillins) Principles of action - Affinity for PBP s Permeability properties
More informationDetecting Carbapenemase-Producing Enterobacteriaceae: why isn t there a single best method?
Detecting Carbapenemase-Producing Enterobacteriaceae: why isn t there a single best method? Professor Neil Woodford Antimicrobial Resistance & Healthcare Associated Infections (AMRHAI) Reference Unit Crown
More informationMHSAL Guidelines for the Prevention and Control of Antimicrobial Resistant Organisms (AROs) - Response to Questions
MHSAL Guidelines for the Prevention and Control of Antimicrobial Resistant Organisms (AROs) - Response to Questions Dr. Andrew Walkty Medical Microbiologist, Diagnostic Services Manitoba (DSM) June. 17,
More informationEVALUATION OF METHODS FOR AMPC β-lactamase IN GRAM NEGATIVE CLINICAL ISOLATES FROM TERTIARY CARE HOSPITALS
Indian Journal of Medical Microbiology, (2005) 23 (2):120-124 Brief Communication EVALUATION OF METHODS FOR AMPC β-lactamase IN GRAM NEGATIVE CLINICAL ISOLATES FROM TERTIARY CARE HOSPITALS S Singhal, T
More informationThis material is supported in part by unrestricted educational grants from: Abbott, Bayer HealthCare, Merck Frosst, Roche Diagnostics, and Wyeth Inc.
Division of Medical Microbiology Department of Laboratory Medicine and Pathology University of Alberta Hospital and Stollery Children's Hospital Antibiogram 2006 This material is supported in part by unrestricted
More informationMALDI-TOF MS: a new tool to rapidly assess antibiotic susceptibility
MALDI-TOF MS: a new tool to rapidly assess antibiotic susceptibility Sören Schubert, MD Max von Pettenkofer-Institut Ludwig-Maximilians-University (LMU) Munich, Germany Learning Objectives After this presentation,
More informationGuidance on screening and confirmation of carbapenem resistant Enterobacteriacae (CRE) December 12, 2011
Guidance on screening and confirmation of carbapenem resistant Enterobacteriacae (CRE) December 12, 2011 Objectives: To discuss the guidelines for detection of CRE in the laboratory setting. To review
More informationCefotaxime Rationale for the EUCAST clinical breakpoints, version th September 2010
Cefotaxime Rationale for the EUCAST clinical breakpoints, version 1.0 26 th September 2010 Foreword EUCAST The European Committee on Antimicrobial Susceptibility Testing (EUCAST) is organised by the European
More informationPhenotypic Detection Methods of Carbapenemase Production in Enterobacteriaceae
ISSN: 2319-7706 Volume 4 Number 6 (2015) pp. 547-552 http://www.ijcmas.com Original Research Article Phenotypic Detection Methods of Carbapenemase Production in Enterobacteriaceae Sathya Pandurangan 1,
More informationActivity of Ceftolozane/Tazobactam Against a Broad Spectrum of Recent Clinical Anaerobic Isolates
AAC Accepts, published online ahead of print on 25 November 2013 Antimicrob. Agents Chemother. doi:10.1128/aac.02253-13 Copyright 2013, American Society for Microbiology. All Rights Reserved. Activity
More informationOvercoming the PosESBLities of Enterobacteriaceae Resistance
Overcoming the PosESBLities of Enterobacteriaceae Resistance Review of current treatment options Jamie Reed, PharmD Pharmacy Grand Rounds August 28, 2018 Rochester, MN 2018 MFMER slide-1 Disclosure No
More informationAcademic Perspective in. David Livermore Prof of Medical Microbiology, UEA Lead on Antibiotic resistance PHE
Academic Perspective in Emerging No, we can t Issues treat of carbapenemase Resistance and ESBL in Gram-ve producers Bacteria based on MIC David Livermore Prof of Medical Microbiology, UEA Lead on Antibiotic
More informationReceived 30 March 2005; returned 16 June 2005; revised 8 September 2005; accepted 12 September 2005
Journal of Antimicrobial Chemotherapy (2005) 56, 1047 1052 doi:10.1093/jac/dki362 Advance Access publication 20 October 2005 Evaluation of PPI-0903M (T91825), a novel cephalosporin: bactericidal activity,
More informationDifferentiation of Carbapenemase producing Enterobacteriaceae by Triple disc Test
Original article: Differentiation of Carbapenemase producing Enterobacteriaceae by Triple disc Test Manish Bansal 1, Nitya Vyas 2, Babita Sharma 3, R.K.Maheshwari 4 1PG Resident, 2 Professor, 3 Assistant
More informationInsert for Kit 98006/98010/ KPC/Metallo-B-Lactamase Confirm Kit KPC+MBL detection Kit KPC/MBL and OXA-48 Confirm Kit REVISION: DBV0034J
Insert for Kit 98006/98010/98015 KPC/Metallo-B-Lactamase Confirm Kit KPC+MBL detection Kit KPC/MBL and OXA-48 Confirm Kit REVISION: DBV0034J DATE OF ISSUE: 09.02.2017 LANGUAGE: English FOR IN VITRO DIAGNOSTIC
More informationNature and Science 2017;15(10)
Evaluation of Substrate Profile Test for Detection of Metallobetalactamses among Imipenem Resistant Clinical Isolates of Gram Negative Bacteria Tarek El-said El-Banna, Fatma Ibrahim Sonboland Eslam Shaaban
More informationTigecycline activity tested against 26, 474 bloodstream infection isolates: a collection from 6 continents
Diagnostic Microbiology and Infectious Disease 52 (2005) 181 186 www.elsevier.com/locate/diagmicrobio Tigecycline activity tested against 26, 474 bloodstream infection isolates: a collection from 6 continents
More informationSurveillance of antimicrobial susceptibility of Enterobacteriaceae pathogens isolated from intensive care units and surgical units in Russia
Feb. 2016 THE JAPANESE JOURNAL OF ANTIBIOTICS 69 1 41 41 Surveillance of antimicrobial susceptibility of Enterobacteriaceae pathogens isolated from intensive care units and surgical units in Russia IRINA
More informationClinical Microbiology Newsletter
Clinical Microbiology Newsletter $88 Vol. 30, No. 10 www.cmnewsletter.com May 15, 2008 Newer β-lactamases: Clinical and Laboratory Implications, Part I * Ellen Smith Moland, B.S.M.T., Soo-Young Kim, M.D.,
More informationDetection of Carbapenem Resistant Enterobacteriacae from Clinical Isolates
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 5 Number 5 (2016) pp. 864-869 Journal homepage: http://www.ijcmas.com Original Research Article http://dx.doi.org/10.20546/ijcmas.2016.505.089
More informationWhy some tests are no longer recommended
8 th July 2014 Why some tests are no longer recommended Robin A Howe, Cardiff, UK Antimicrobial use in Primary Care Tests that are no longer recommended Burkholderia cepacia complex testing Stenotrophomonas
More informationCARBAPENEMASE PRODUCING ENTEROBACTERIACEAE
CARBAPENEMASE PRODUCING ENTEROBACTERIACEAE Veroniek Saegeman Veroniek Saegeman UZLeuven Carbapenemase producing Enterobacteriaceae (CPE) Introduction on antibiotic resistance Classification of ß-lactamases
More informationSpectrum of vancomycin and susceptibility testing
Spectrum of vancomycin and susceptibility testing Olivier Denis Service de Microbiologie Laboratoire de bactériologie Service de Microbiologie Hôpital Erasme Glycopeptides Vancomycin 1958 < Amycolatopsis
More information1430 West McCoy Lane Santa Maria, CA p:
091217TR HardyCHROM BluEcoli 1 HardyCHROM Candida 2 HardyCHROM ECC 3 HardyCHROM ESBL 4 HardyCHROM Listeria 5 HardyCHROM MRSA 6 HardyCHROM O157 7 HardyCHROM Salmonella 8 HardyCHROM SS NoPRO 9 HardyCHROM
More informationβ- Lactamase Gene carrying Klebsiella pneumoniae and its Clinical Implication
Prevalence of Carbapenem-Hydrolyzing β- Lactamase Gene carrying Klebsiella pneumoniae and its Clinical Implication David Alcid M.D Balaji Yegneswaran M.D. Wanpen Numsuwan Introduction Klebsiella pneumoniae
More informationNew Mechanisms of Antimicrobial Resistance and Methods for Carbapenemase Detection
New Mechanisms of Antimicrobial Resistance and Methods for Carbapenemase Detection Stephen G. Jenkins, PhD, F(AAM), D(ABMM) Professor of Pathology and Laboratory Medicine Professor of Pathology in Medicine
More informationALERT. Clinical microbiology considerations related to the emergence of. New Delhi metallo beta lactamases (NDM 1) and Klebsiella
ALERT Clinical microbiology considerations related to the emergence of New Delhi metallo beta lactamases (NDM 1) and Klebsiella pneumoniae carbapenemases (KPC) amongst hospitalized patients in South Africa
More informationCarbapenems and Enterobacteriaceae
Title Carbapenems and Enterobacteriaceae Presenter s details NHLS Dr Khine Swe Swe/Han FC Path ( Micro), SA MMed( micro), SA DTMH(Wits univ),sa PDIC(Stellen univ)sa MB,BS(Yangon),Myanmar Pathologist,Consultant/Lecturer,
More informationMethodological and interpretative problems in antimicrobial susceptiblity tests of P. aeruginosa
Methodological and interpretative problems in antimicrobial susceptiblity tests of P. aeruginosa Y. Glupczynski Laboratoire de bactériologie Cliniques Universitaires UCL de Mont-Godinne Université Catholique
More informationEducational Workshops 2016
Educational Workshops 2016 Keynote CPE Screening We are grateful to Dr Andrew Dodgson, Consultant Microbiologist, Public Health England and Central Manchester Hospitals NHS Foundation Trust Terminology
More informationRecommendations for Dilution Susceptibility Testing Concentrations of the Cefoperazone-Sulbactam Combination
JOURNAL OF CLINICAL MICROBIOLOGY, Sept. 1987. p. 1725-1729 0095-1137/87/091725-05$02.00/O Copyright (O 1987, American Society for Microbiology Vol. 25, No. 9 In Vitro Antimicrobial Spectrum, Occurrence
More informationHUSRES Annual Report 2009 Martti Vaara
HUSRES Annual Report 2009 Martti Vaara www.huslab.fi www.intra.hus.fi Martti Vaara, 2/2010 1 The basis of this HUSRES 2009 report is the HUSLAB/Whonet database 2009, which contains susceptibility data
More informationDetermining the Optimal Carbapenem MIC that Distinguishes Carbapenemase-Producing
AAC Accepted Manuscript Posted Online 8 August 2016 Antimicrob. Agents Chemother. doi:10.1128/aac.00838-16 Copyright 2016, American Society for Microbiology. All Rights Reserved. 1 1 2 Determining the
More informationProtein Synthesis Inhibitors
Frank Lowy Protein Synthesis Inhibitors This lecture discusses a diverse group of antibiotics that are grouped together because they all have a common mechanism of action they are protein synthesis inhibitors.
More information(multidrug-resistant Pseudomonas aeruginosa; MDRP)
220 2009 (multidrug-resistant Pseudomonas aeruginosa; MDRP) 21 4 1 21 10 4 amikacin (AMK), imipenem/cilastatin (IPM), ciprofloxacin (CPFX) multidrug-resistant Pseudomonas aeruginosa (MDRP) CHROMagar TM
More informationDetecting CRE. what does one need to do?
5 th ICAN Conference, Harare 4 th November 2014 Room 2: 10:30-12:00 Detecting CRE (Carbapenem-resistant Enterobacteriaceae) what does one need to do? Dr Nizam Damani Associate Medical Director Infection
More informationCarbapenem Disks on MacConkey agar as screening methods for the detection of. Carbapenem-Resistant Gram negative rods in stools.
JCM Accepts, published online ahead of print on 7 November 2012 J. Clin. Microbiol. doi:10.1128/jcm.02878-12 Copyright 2012, American Society for Microbiology. All Rights Reserved. 1 2 Carbapenem Disks
More informationPanel discussion-aidc 2017 Rapport with the microbiology lab-how it helps your patient
Panel discussion-aidc 2017 Rapport with the microbiology lab-how it helps your patient Dr Ram Gopalakrishnan Dr S Nandini Moderator Dr V R Yamunadevi Scenario 1 60 year old male patient admitted in ICU
More informationOther β-lactam. A. Carbapenems:
A. Carbapenems: Other β-lactam Carbapenems are synthetic β-lactam antibiotics Differ in structure from the penicillins in that the sulfur atom of the thiazolidine ring. Imipenem, meropenem, doripenem,
More informationCarbapenemases in Enterobacteriaceae: Prof P. Nordmann Bicêtre hospital, South-Paris Med School
Carbapenemases in Enterobacteriaceae: 2012 Prof P. Nordmann Bicêtre hospital, South-Paris Med School March 21, 2012 Trends in Molecular Medecine NDM IMP OXA-48 KPC VIM ALERT VI M KPC KPC NDM I MP OXA-
More informationMacrolides, Clindamycin & Ketolides Polymyxins
Macrolides, Clindamycin & Ketolides Polymyxins Kwan Soo Ko Macrolides - Erythromycin - Azithromycin - Clarithromycin Lincosamides - Lincomycin - Clindamycin Unrelated chemically But, many similar biological
More informationWhat is new in EUCAST South Africa, May, Gunnar Kahlmeter EUCAST Technical Data Coordinator and Webmaster Sweden
What is new in EUCAST 2016 17 South Africa, May, 2016 Gunnar Kahlmeter EUCAST Technical Data Coordinator and Webmaster Sweden What is new in EUCAST 2016/17? New organisms with breakpoints (Addendum 2016)
More informationCefuroxime iv Rationale for the EUCAST clinical breakpoints, version th September 2010
Cefuroxime iv Rationale for the EUCAST clinical breakpoints, version 1.0 26 th September 2010 Foreword EUCAST The European Committee on Antimicrobial Susceptibility Testing (EUCAST) is organised by the
More informationDIVISION OF ANTIINFECTIVE DRUG PRODUCTS (HFD-520) CLINICAL MICROBIOLOGY REVIEW NDA Date review completed: 15 Jun 05
Date company submitted: 15 Dec 04 Date received by CDER: 15 Dec 04 Reviewer: Fred Marsik, Ph.D. Date assigned: 15 Dec 04 NAME AND ADDRESS OF APPLICANT Wyeth Pharmaceuticals Inc. P.O. Box 8299 Philadelphia,
More informationAAC Accepts, published online ahead of print on 13 October 2008 Antimicrob. Agents Chemother. doi: /aac
AAC Accepts, published online ahead of print on 13 October 2008 Antimicrob. Agents Chemother. doi:10.1128/aac.00931-08 Copyright 2008, American Society for Microbiology and/or the Listed Authors/Institutions.
More informationExpert rules in antimicrobial susceptibility testing: State of the art
Expert rules in antimicrobial susceptibility testing: State of the art ESCMID Postgraduate Education Course Antimicrobial Susceptibility Testing and Surveillance: from Laboratory to Clinic Hospital Universitario
More informationCanward James A Karlowsky PhD, Mel DeCorby MSc, Daryl J Hoban PhD, George G Zhanel PhD
Canward 2007 In vitro activities of ceftobiprole and doripenem tested against frequently encountered aerobic and facultative Gram-positive and Gram-negative bacterial pathogens isolated from patients in
More informationIn-House Standardization of Carba NP Test for Carbapenemase Detection in Gram Negative Bacteria
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 01 (2018) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2018.701.342
More informationT&T WG. June 2018 San Diego, CA
T&T WG June 2018 San Diego, CA Co-Chairs Jana Swenson Shelley Campeau Secretary Carey-Ann Burnham Members Andrea Ferrell Janet Hindler Melissa Jones Peggy Kohner Dyan Luper Linda Mann Susan Munro Barth
More informationAntibacterial Antibiotic Resistancein 2016 What Should an Internist Know? Disclosures. Objectives 3/6/2016. Achaogen: Allergan:
Antibacterial Antibiotic Resistancein 2016 What Should an Internist Know? Michael Satlin, MD, MS Assistant Professor of Medicine Division of Infectious Diseases Weill Cornell Medicine March 4, 2016 1 Disclosures
More information#Corresponding author: Pathology Department, Singapore General Hospital, 20 College. Road, Academia, Level 7, Diagnostics Tower, , Singapore
AAC Accepts, published online ahead of print on 21 October 2013 Antimicrob. Agents Chemother. doi:10.1128/aac.01754-13 Copyright 2013, American Society for Microbiology. All Rights Reserved. 1 Title: Escherichia
More informationThe CLSI Approach to Setting Breakpoints
The CLSI Approach to Setting Breakpoints Jean B. Patel, PhD, D(ABMM) Deputy Director, Office of Antimicrobial Resistance Division of Healthcare Quality Promotion National Center for Emerging and Zoonotic
More informationTHE USE OF THE PENICILLINASE-RESISTANT
Therapeutic problems THE USE OF THE PENICILLINASE-RESISTANT PENICILLIN IN THE PNEUMONIAS OF CHILDREN MARTHA D. Yow, MARY A. SOUTH AND CHARLES G. HESS From the Department of Pediatrics, Baylor University
More informationBACTERIOLOGY PROFICIENCY TESTING PROGRAM
BACTERIOLOGY PROFICIENCY TESTING PROGRAM Comprehensive Category January 14, 2013 If you have any questions or comments, please contact either: Dr. Wendy Archinal Dr. Kimberlee Musser Phone: (518) 474-4177
More informationSensitive and specific Modified Hodge Test for KPC and metallo-beta-lactamase
JCM Accepts, published online ahead of print on 19 October 2011 J. Clin. Microbiol. doi:10.1128/jcm.05602-11 Copyright 2011, American Society for Microbiology and/or the Listed Authors/Institutions. All
More informationSensitivity of Surveillance Testing for Multidrug-Resistant Gram-Negative Bacteria in the
JCM Accepts, published online ahead of print on 20 August 2014 J. Clin. Microbiol. doi:10.1128/jcm.02369-14 Copyright 2014, American Society for Microbiology. All Rights Reserved. 1 2 Sensitivity of Surveillance
More informationThe role of an AMR reference laboratory
The role of an AMR reference laboratory Professor Neil Woodford Antimicrobial Resistance & Healthcare Associated Infections (AMRHAI) Reference Unit Crown copyright Primary purpose: regional AMR threats
More informationISSN X (Print) Research Article
Scholars Journal of Applied Medical Sciences (SJAMS) Sch. J. App. Med. Sci., 2014; 2(2D):65-69 Scholars Academic and Scientific Publisher (An International Publisher for Academic and Scientific Resources)
More informationRapid identification and resistance assessment: The future is mass spectrometry
Rapid identification and resistance assessment: The future is mass spectrometry Dr Sanmarié Schlebusch Director of Microbiology Mater Pathology Brisbane Outline Introduction Plug and play Pre-prep and
More informationSusceptibility rates in Latin American nations: report from a regional resistance surveillance program (2011)
b r a z j i n f e c t d i s. 2 0 1 3;1 7(6):672 681 The Brazilian Journal of INFECTIOUS DISEASES www.elsevier.com/locate/bjid Original article Susceptibility rates in Latin American nations: report from
More informationIn Vitro Susceptibility Pattern of Cephalosporin- Resistant Gram-Negative Bacteria
In Vitro Susceptibility Pattern of Cephalosporin- Resistant Gram-Negative Bacteria Warunee Punpanich MD*, Worraporn Tantichattanon MD**, Siriporn Wongwatcharapaiboon MD**, Vipa Treeratweeraphong BSc, MSc***
More informationDetection of carbapenemases in Enterobacteriaceae: a challenge for diagnostic microbiological laboratories
REVIEW 10.1111/1469-0691.12678 Detection of carbapenemases in Enterobacteriaceae: a challenge for diagnostic microbiological laboratories J. Hrabak, E. Chudackova and C. C. Papagiannitsis Department of
More informationBACTERIOLOGICAL PROFILE OF DIABETIC FOOT INFECTIONS
ejpmr, 2018,5(6), 631-635 Khadse et al. SJIF Impact Factor 4.897 Research Article EUROPEAN JOURNAL OF PHARMACEUTICAL AND MEDICAL RESEARCH ISSN 2394-3211 www.ejpmr.com EJPMR BACTERIOLOGICAL PROFILE OF DIABETIC
More informationEmergence of Klebsiella pneumoniae ST258 with KPC-2 in Hong Kong. Title. Ho, PL; Tse, CWS; Lai, EL; Lo, WU; Chow, KH
Title Emergence of Klebsiella pneumoniae ST258 with KPC-2 in Hong Kong Author(s) Ho, PL; Tse, CWS; Lai, EL; Lo, WU; Chow, KH Citation International Journal Of Antimicrobial Agents, 2011, v. 37 n. 4, p.
More informationPEDIATRIC PHARMACOTHERAPY
PEDIATRIC PHARMACOTHERAPY Volume 23 Number 6 June 2017 C Ceftaroline: An Alternative Broad Spectrum Antibiotic for Pediatric Infections Marcia L. Buck, PharmD, FCCP, FPPAG, BCPPS eftaroline was approved
More informationClinical Management of Infections Caused by Enterobacteriaceae that Express Extended- Spectrum β-lactamase and AmpC Enzymes
56 Clinical Management of Infections Caused by Enterobacteriaceae that Express Extended- Spectrum β-lactamase and AmpC Enzymes Patrick N. A. Harris, BSc, MBBS, MRCP, DTM&H, FRACP, FRCPA 1 1 Infection and
More informationOverview of Tigecycline and Its Role in the Era of Antibiotic Resistance
BJID 2006; 10 (June) 203 Overview of Tigecycline and Its Role in the Era of Antibiotic Resistance Flávia Rossi, Denise Andreazzi University of São Paulo, LIM 03; São Paulo, SP, Brazil The increasing antimicrobial
More informationEndocardite infectieuse
Endocardite infectieuse 1. Raccourcir le traitement: jusqu où? 2. Proposer un traitement ambulatoire: à partir de quand? Endocardite infectieuse A B 90 P = 0.014 20 P = 0.0005 % infective endocarditis
More informationHOW BUGS COMBAT DRUGS: ANTIMICROBIAL RESISTANCE MECHANISMS IN THE MODERN ERA. Romney Humphries, PhD D(ABMM)
HOW BUGS COMBAT DRUGS: ANTIMICROBIAL RESISTANCE MECHANISMS IN THE MODERN ERA Romney Humphries, PhD D(ABMM) Section Chief, UCLA Clinical Microbiology Los Angeles CA rhumphries@mednet.ucla.edu WHAT WE WILL
More informationCarbapenemase-producing Enterobacteriaceae
Carbapenemase-producing Enterobacteriaceae 2012 CNR Associé Résistance aux Antibiotiques Prof. P. Nordmann Carbapenemases in Enterobacteriaceae May, 2012 Penicillins Cephalosporins Carbapenems Extended-spectrum
More informationology of borderline oxacillin-resistant Staphylococcus aureus in pediatric cystic fibrosis..
Epidemiology of borderline oxacillin-resistant Staphylococcus aureus in pediatric cystic fibrosis. Timothy Ronan Leahy, Yvonne Yau, Eshetu Atenafu, Mary Corey, Felix Ratjen, Valerie Waters To cite this
More informationInt.J.Curr.Microbiol.App.Sci (2016) 5(1):
ISSN: 2319-7706 Volume 5 Number 1(2016) pp. 200-208 Journal homepage: http://www.ijcmas.com Original Research Article http://dx.doi.org/10.20546/ijcmas.2016.501.018 Incidence of Beta Lactamases Mediated
More information(Plasmid mediated) Carbapenemases. Timothy R. Walsh, Cardiff University, Wales
(Plasmid mediated) Carbapenemases Timothy R. Walsh, Cardiff University, Wales What is a carbapenemase? How much carbapenem do they need to breakdown before they are called a carbapenemase? ESBL-enzymes
More informationAntibiotic Classification Antibiotic Classification and Modes of Action
and Modes of Action Part # 60-00415-0 1 biomérieux, the blue logo and VITEK are used, pending and/or registered trademarks belonging to biomérieux SA or one of its subsidiaries. CLSI is a registered trademark
More informationCarbapenemases: the Versatile -Lactamases
CLINICAL MICROBIOLOGY REVIEWS, July 2007, p. 440 458 Vol. 20, No. 3 0893-8512/07/$08.00 0 doi:10.1128/cmr.00001-07 Copyright 2007, American Society for Microbiology. All Rights Reserved. Carbapenemases:
More informationDisclosure. Objectives. Evolution of β Lactamases. Extended Spectrum β Lactamases: The New Normal. Prevalence of ESBL Mystic Program
47 th Annual Meeting August 2-4, 2013 Orlando, FL Extended Spectrum β Lactamases: The New Normal Disclosure I do have a vested interest in or affiliation with the following companies or organizations Triax
More informationMolecular characterisation of CTX-M-type extendedspectrum β-lactamases of Escherichia coli isolated from a Portuguese University Hospital
EJHP Science Volume 17 2011 Issue 3 P. 1-5 2011 Pharma Publishing and Media Europe. All rights reserved 1781-7595 25 www.ejhp.eu Molecular characterisation of CTX-M-type extendedspectrum β-lactamases of
More information