Utilising edna technology for detection of Aedes aegypti DNA in water

Transcription

1 Utilising edna technology for detection of Aedes aegypti DNA in water Nadia Bannerman Environmental Health Officer Gympie Regional Council EHA (QLD) Inc 77th Annual State Conference Tuesday, 4 September 2018

2 Environmental DNA (edna) Just like Every contact leaves a trace principle every living creature leaves traces of their DNA in their surrounding environment through skin cells, hairs and bodily secretions, this is called environmental DNA or edna.

3 Environmental DNA (edna) Pros Cryptic life stages Problematic morphological identification No prior assessment Any life-form DNA persistence < 3 months Cons X Dead or alive X Stages of development X Density Initial step in surveillance.

4 EnviroDNA laboratory EnviroDNA laboratory is based in Melbourne. Dr Andrew Weeks (University of Melbourne) is leading mosquito research. Database of genetic assays including Aedes aegypti, Aedes albopictus and Aedes notoscriptus; $ (excl GST) per test (per species); Worked with WA Health and Federal Department of Agriculture and Water Resources at Perth and Melbourne airports in Gympie Regional Council is the first Council to trial edna as a surveillance tool.

5 Goomeri Approved systematic inspection program of rainwater tanks (s134, Local Government Act 2009) April, May and June 2018 First detected in 1998 House-to-house 2004, 2010, 2014 Pot plant saucers Bird baths Buckets Ice cream containers Animal water dishes Rapid Surveillance Vector Program (RSVP) 2016

6 Approved systematic inspection program Compliance with Section 2P of the Public Health Regulation 2005: edna sampling of non-compliant rainwater tanks

7 Rainwater tank inspections in Goomeri 314 residences

8 Notice of Entry

9 Use of rainwater tanks No 8% 92% of residences have rainwater tanks Average of 2 tanks per residence 553 tanks in total Yes 92%

10 Types of rainwater tanks

11 Non-compliant rainwater tanks 42%

12 Inlets

13 Outlets

14 Derelict / rusty tanks

15 Sampling begins Paul Endres (SCPHU), Dr Brendan Trewin (CSIRO), Nadia Bannerman (GRC)

16 Sampling Kit

17 Sampling Stuart Heggie (SCPHU), Brendan Trewin (CSIRO) Tracey Pottle (GRC)

18 Filtering samples 1 2 Air 50ml x 10 3 Ice

19 pg Lessons learned Very tiring! Organic matter / sediment Cross contamination Wi-Fi camera vs Mirror.

20

21 edna results 19 positive 16 negative 1 lost

22 edna results Ae. aegypti DNA positives: Highest picograms (pg) count Lowest pg count 0.80

23 edna results 15 new positive locations (no larvae present): School grounds (8 of 12 tanks non-compliant) Town centre property (4 tanks non-compliant) Edge of town (semi-rural)(6 tanks non-compliant) High set house (5 tanks non-compliant) Negative tanks next to positive Sealed tank? Confirms Rapid Surveillance Vector Program (RSVP) results (2016). 44 x Public Health Orders to be re-inspected in October 2018.

24 edna results

25 Aim Aedes aegypti population connectivity along major transport corridors in southern Queensland Brendan Trewin Gordana Rasic Nigel Beebe Paul De Barro o o o o 12 weeks of surveillance Goomeri, Murgon, Wondai, Monto, Biggenden, Mundubbera, Gayndah, Gin Gin 5 BG traps and 20 ovitraps per town Collecting 30 Aedes aegypti specimens for DNA testing Gin Gin NIL Aedes aegypti detected!

26 Big thanks to Sunshine Coast Public Health Unit Jarod Butler, Paul Endres, Stuart Heggie, Rachel Harris, Michelle Maskill