Oris Assays: An Innovative Platform for the Study of Cell Migration & Cell Invasion
|
|
|
- Stephen Grant
- 6 years ago
- Views:
Transcription
1 Bringing Science to the Surface Oris Assays: An Innovative Platform for the Study of Cell Migration & Cell Invasion AMS Biotechnology (Europe) info@amsbio.com UK +44 (0) CH +41 (0) DE +49 (0)
2 Oris and Oris Pro Assays Create a cell-free, central Detection Zone in the center of each well into which cell movement can occur. Enable the study of both cell migration (2D) and cell invasion (3D). Offer various options for pre-coated or coat-your-own Extracellular Matrix formats. Allow collection of real-time data using multiple fluorescent stains and quantify using a versatile range of image based or microplate reader instrumentation. Facilitate use of automated liquid handling equipment for cell seeding and offer unlimited access to wells from cell seeding to data readout. Pre-Migration (t= 0) Cell Migration Post-Migration (t= 21 hrs) # Cells in Detection Zone Time (hrs) n = 9 wells / time point
3 Oris Cell Motility Assay Product Line Product Name Coating Detection Zone Format Instrument Compatibility Oris Pro Cell Migration Assays Oris Pro Cell Invasion Assays Tissue Culture Treated Collagen I Coated Collagen I Coated Biocompatible Gel HCS/HCI Inverted Microscope Tissue Culture Treated Oris Cell Migration Assays Collagen I Coated Fibronectin Coated Oris Cell Seeding Stoppers (pre-populated) TriCoated Oris Cell Migration Assembly Kits Universal (TC Treated) FLEX (TC Treated) Oris Cell Seeding Stoppers (not pre-populated) HCS/HCI Inverted Microscope Microplate Reader Oris Cell Invasion Assays BME Collagen I Oris Cell Seeding Stoppers (not pre-populated) Oris Cell Seeding Stoppers (pre-populated)
4 Features and Benefits More Data per Well analyze cells treated with multiple fluorescent probes, labels or stains using a microplate reader, microscope or high content imaging system. Real-time Monitoring monitor changes in cell movement and morphology in real-time. Flexible Data Capture and Analysis select from a wide range of instrumentation, stains and software to achieve your experimental goals. Creative Assay Design select from our range of pre-coated plates or coat any ECM on the plate yourself -- to create a format for migration, invasion or wound closure assays. Membrane/Insert- free Cell Invasion & Migration observe cells directly without cumbersome transmembrane inserts; compatible with HTS and HCS applications. Reproducible Results achieve lower well-to-well CV s with the unique Oris assay design than with scratch assays. Z-factors attractive for HTS.
5 Configuring your Oris Assay Decide on a Cell Migration (2D) or Cell Invasion (3D) Assay Select the Oris Pro or Oris Format Select an ECM Choose Instrument, Method and Software for Data Capture and Analysis Optimize Assay Parameters Perform Study
6 Decide on a Cell Migration (2D) or Cell Invasion (3D) Assay Select the Oris Pro or Oris Format Select an ECM Choose Instrument, Method and Software for Data Capture and Analysis Optimize Assay Parameters Perform Study Decide on a Cell Migration (2D) or Cell Invasion (3D) Assay
7 Oris Cell Migration Assay Platform
8 Oris Cell Invasion Assay Platform
9 Select the Oris or Oris Pro Format Decide on a Cell Migration (2D) or Cell Invasion (3D) Assay Select an ECM Choose Instrument, Method and Software for Data Capture and Analysis Optimize Assay Parameters Perform Study Select the Oris Pro or Oris Format
10 Capabilities of Oris Pro & Oris Product Line Biocompatible Gel Oris Cell Seeding Stopper Oris Pro Cell Migration Assays Oris Cell Migration Assays Variety of ECM Coatings High Content Screening/Imaging Inverted Microscope Microplate Reader Ability to Perform: time-lapse experiments, high resolution imaging, and multiplexing using different fluorophores Compatibility with Automated Liquid Handling Equipment High Z Factor and Low Variability Compatible with Adherent Cells and Cell Migration and Invasion Formats
11 Oris Pro Biocompatible Gel Proven Non-Toxic Absorbance (490nm) Oris TM Pro-Collagen I CellTiter 96 HT1080 HUVEC MDA-MB-231 No BCG BCG Fluorescence (540/590) Oris TM Pro Collagen I Vibrant TM 300 No BCG BCG HT-1080 HUVEC MDA-MB-231 CellTiter 96 AQ ueous One Solution Cell Proliferation Assay (Promega) - Measures viability as metabolic function through reduction of formazan Vybrant TM Cytotoxicity Assay (Molecular Probes) - Measures glucose 6-phosphate dehydrogenase (G6PD) released from damaged cells
12 Equivalent Performance of Oris and Oris Pro % Closure (average) Control 100 M UO126 HT-1080 cells 50 M Blebbistatin 50 M Y Oris TM Pro Collagen I Oris TM Collagen I 1 M Cytochalasin D HT-1080 cell migration after 18hr was identical in both assays (measured by % area closure of the Detection Zones). 4 different classes of cell migration inhibitors indicated substantially equivalent effects on HT-1080 cell migration in both assays.
13 Equivalent Performance of Oris and Oris Pro Overlapping dose-response curves and IC 50 values in Oris and Oris Pro assay formats for Cytochalasin D treated cells
14 Select an ECM Decide on a Cell Migration (2D) or Cell Invasion (3D) Assay Select the Oris Pro or Oris Format Choose Instrument, Method and Software for Data Capture and Analysis Optimize Assay Parameters Perform Study Select an ECM
15 Oris TriCoated Plate Enables Sampling of ECMs Oris Cell Migration Assay TriCoated Plate 2.8% 14.7% 3.2% Tissue Culture Treated (uncoated) Collagen I Fibronectin ECM Z' factor TC 0.79 Collagen 0.90 Fibronectin 0.31 MDS IsoCyte U0126 MEK Inhibitor
16 Use of Oris Assembly Kits Enables Study of ECMs
17 Oris Invasion Assays Permit Optimization of ECM Overlay Increasing the concentration of the BME overlay affects the ability of cells to invade.
18 Choose Instrument, Method and Software for Data Capture and Analysis Decide on a Cell Migration (2D) or Cell Invasion (3D) Assay Select the Oris Pro or Oris Format Select an ECM Optimize Assay Parameters Perform Study Choose Instrument, Method and Software for Data Capture and Analysis
19 Oris Assays Enable Versatile Data Readout Microscopes Inverted Microscopes (i.e., fluorescence, bright field, phase contrast) Fluorescence Plate Readers (bottom probe) VICTOR3 V (PerkinElmer) Synergy HT & FLx800 (BioTek Instruments) Safire2 & GENios Pro (Tecan) POLARstar Omega (BMG LABTECH) Analyst (LJL Biosystems) Paradigm (Beckman Coulter) Odyssey & Aerius Infrared Imaging Systems (LI-COR) HCS/HCI Imaging Systems Opera & Operetta (PerkinElmer) Acumen ex 3 (TTP LabTech) ArrayScan VTI HCS Reader (Cellomics) ImageXpress & Isocyte (Molecular Devices) Pathway Bioimager (BD Biosciences) IN Cell Analyzer (GE)
20 Area Closure to Examine Cell Migration in Detection Zone PE Operetta
21 Cell Count to Assess Cell Migration in Detection Zone PE Opera
22 Microplate Cytometer to Analyze Cell Migration in Detection Zone HT-1080 Cell Migration (µm 2 ) TTP LabTech Acumen ex 3 27
23 Flexible Stain Options to Measure Cell Migration DAPI Calcein AM TRITC-phalloidin Control 16 hour serum Fluorescent Label Z S:N DAPI Calcein AM TRITC-phalloidin Z-factor of J Biomol Screen. - Oris Assays achieve similar Z factors on microplate cytometers using DAPI, Calcein AM and TRITC-phalloidin stains
24 Convenient Assay Options to Measure Endpoint Cell Migration Cell Migration Images: MDA-MB Cell Migration Data: MDA-MB-231 Oris Cell Migration Assay Collagen I Coated Kit Mean Migration (RFUs) hr 20 hr Average RFU S.D % CoV Signal-Noise 8.9 Z-factor 0.59
25 Dynamic Assay Options to Measure Kinetic Cell Migration 3 sirna pools against different PtdIns pathway targets. Fluorescence Readings obtained for 48 hr (every 20 min) for cells migrating on fibronectin. Data presented as average AUC. Cells transfected with sirna pools A & B demonstrated a similar drop in migration. BMG LABTECH POLARstar Omega sirna transfected U2OS cells labeled with DiI-C16 sirna pool C had a more pronounced knockdown effect than pools A & B.
26 ImageJ Software Compatibility to Determine Cell Number and Area Closure Particle Analysis - DAPI Stain (MDA-MB-231 Cells) Area Closure Analysis - Phase Contrast (HT-1080 Cells)
27 Optimize Assay Parameters Decide on a Cell Migration (2D) or Cell Invasion (3D) Assay Select the Oris Pro or Oris Format Select an ECM Choose Instrument, Method and Software for Data Capture and Analysis Perform Study Optimize Assay Parameters
28 Oris Assay Optimization Parameters Identify cell seeding density to achieve 95% -100% confluence upon initiation of migration. Choose appropriate ECM for your cells. Determine the appropriate time needed to observe robust cellular movement into Detection Zone. Accurately acquire data by selecting appropriate stain (live vs. fixed; nuclear vs. cytoskeletal) and choice of instrumentation. F-actin and Focal Adhesion Staining
29 Identify Appropriate Cell Seeding Density Acceptable Not Acceptable A549 Cells Cellomics ArrayScan
30 Perform Study Decide on a Cell Migration (2D) or Cell Invasion (3D) Assay Select the Oris Pro or Oris Format Select an ECM Choose Instrument, Method and Software for Data Capture and Analysis Optimize Assay Parameters Perform Study
31 Oris Assays Enable Cell Migration Analysis of c-met Pathway Migration of Calcein AM stained A549 cells on Collagen I coated plates was quantitated by use of a BioTek Synergy fluorescence microplate reader. [PHA ] (Log M)
32 Multiple Approaches to Studying c-met Inhibition using Cell Migration
33 Multiplexed Assay of ERK Phosphorylation and its Impact on Cell Migration Cell Migration (RED) ERK Phosphorylation (GREEN) Simultaneous determination of effects of MEK and PI 3 kinase inhibition on cell migration (IRDye680 RED) and ERK phosphorylation (IRDye800cw GREEN) using multiplexed staining in a LI-COR Odyssey system with the Oris Detection Mask attached. Cell Migration is inhibited equivalently by the MEK inhibitor U0126 and the PI 3 kinase inhibitor Ly while inhibition of ERK phosphorylation is more effectively and completely blocked by the MEK inhibitor U0126 than by the PI 3 kinase inhibitor Ly
34 Oris Cell Invasion Assays Provide a 3-D Environment Invasive - T=0 hrs Non-Invasive -Swiss Albino Cells T=48 hrs (no FBS) T=48 hrs (10% FBS) T=0 hrs T=48 hrs (no FBS) T=48 hrs (10% FBS) Mean Fluorescence Units hrs 48hrs no FBS 10% FBS no FBS 10% FBS HT1080 cells T3 cells Supplements in the Oris BME overlay
35 Oris Invasion Assays Enable Characterization of Invadopodia A. B. C. Indirect Immunofluorescence to Identify Invadopodia Markers: F-actin and Cortactin co-localized (A) Expression of MMP-9 protease (B) Expression of Cathepsin B (C) HT-1080 cells were imaged after 48 hours of 3-D invasion into BME.
36 Oris Invasion Assays Capture Cells in the Z-axis HT-1080 Cell Invasion in Oris Pro Collagen Invasion Assay
37 Oris Invasion Assays Capture Cells in the Z-axis HT-1080 Cells 72 h Invasion 4 mg/ml Collagen I Number of Invaded Cells Microns Above Plate Surface
38 Oris Invasion Assays Help Unravel Complex Signal Transduction Mechanisms TGF- 1 & EGF synergize to promote HaCaT II-4 invasion into BME EGFR Inhibitor P38 Kinase Inhibitor MEK Inhibitor Signal transduction inhibitors attenuate TGF- 1 & EGF induced invasion Plasminogen Activator Inhibitor -1 (PAI-1) sirna knockdown attenuates HaCaT II-4 invasion into BME in both basal and stimulated conditions
39 Cell Motility Assay Competition - Scratch Assays Oris Assay Area Closure: 87-89% Scratch Assay Area Closure: 69-77% Oris Assay %CV: % Scratch Assay % CV: % Oris Assay: ECM intact Scratch Assay: scratching damages the ECM
40 Cell Motility Assay Competition Transmembrane Assays Oris Assays permit real-time visualization and multiplexed staining of cells. Oris Invasion Assays provide a more physiologically relevant 3D environment and allow collection of Z-stack images. Oris Assays allow rapid evaluation using a microplate reader.
41 Cell Motility Assay Comparison Scratch Assays Transmembrane Assays Oris Assays Benefits Limitations Cells are Damaged During Scratch Formation ECM can be Damaged When Creating Scratch Inconsistent Scratch Size Cannot Use Microplate Reader Cells Move in Defined Direction Choice of ECM High Resolution Images are Difficult to Obtain Cannot Perform Time-Lapse Experiments Variable Results Provide Chemical Gradient Choice of ECM Ability to Use Microplate Reader Not Compatible with Non-Adherent Cells Not Suitable for Chemotaxis High Resolution Imaging Multiplex Using Different Fluorophores Flexibility in Readout (Microplate Reader, Inverted Microscope, High Content Screening & High Content Imaging) Choice of ECM
42 New - Oris Pro 384 Miniaturization of Oris Pro to 384-well assay Particularly well-suited for higher density screening of compound libraries Seed BCG Dissolves to Reveal Detection Zone Analyze Cell Migration by HCS/HCI Instrumentation Achieve Robust Z 10,000 cells/well; 2.5 x magnification; TRITC-phalloidin stained HT-1080 cells Z = 0.76 after 19 h migration on TC treated surface
43 How Oris Assays Can Work for You Assay formats are suitable for partial or full automation. Offer flexibility to select the most optimal combination of instrumentation, stains and software for data capture and analysis. Enables the choice of migration (2D) and invasion (3D) assay formats. Select the ECM best suited for your experiment. Application and Technical Support fromamsbio and instrument vendors. Demonstrated capabilities for both target analysis and compound screening.
44 ! !41 7!4" 2# 2$% &'()*+,**((&-'().-//-(--&'()-.(,,**
Features & Benefits of the Oris Cell Migration Assay
Adherent cell migration without cell culture inserts The Oris Cell Migration Assay* is designed with a unique cell seeding stopper, detection mask, and stopper tool. There is no Transwell membrane insert
Cell Migration and Invasion Assays INCUCYTE LIVE-CELL ANALYSIS SYSTEM. Real-time automated measurements of cell motility inside your incubator
Cell Migration and Invasion Assays INCUCYTE LIVE-CELL ANALYSIS SYSTEM Real-time automated measurements of cell motility inside your incubator See the whole story Real-time cell motility visualization and
Overview of methodology, tools and reagents for evaluating cell proliferation and invasion using multicellular tumor spheroids.
The Next Step in the Evolution of 3D Culture: Utilizing Extracellular Matrix to Enhance Multicellular Tumor Spheroid Models for Proliferation and Invasion Overview of methodology, tools and reagents for
IncuCyte Scratch Wound 96-Well Real-Time Cell Migration and Invasion Assays
IncuCyte Scratch Wound 96-Well Real-Time Cell Migration and Invasion Assays Meagan Roddy, Thom Nelson, Daniel M. Appledorn, and Vince Groppi Essen BioScience Ann Arbor, Michigan Introduction Cell migration
Introduction. Table 1. Metastatic Tumor Formation
CellPlayer 96-Well Kinetic Cell Migration and Invasion Assays Meagan Roddy, Thom Nelson, Daniel M. Appledorn, and Vince Groppi Essen BioScience Ann Arbor, Michigan Introduction Cell migration is a multistep
SUPPLEMENTARY FIGURES
SUPPLEMENTARY FIGURES Supplementary Figure S1: Fibroblast-induced elongation of cancer cells requires direct contact with living fibroblasts. A. Representative images of HT29-GFP cultured in the presence
SUPPLEMENT. Materials and methods
SUPPLEMENT Materials and methods Cell culture and reagents Cell media and reagents were from Invitrogen unless otherwise indicated. Antibiotics and Tet-certified serum were from Clontech. In experiments
SUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb3021 Supplementary figure 1 Characterisation of TIMPless fibroblasts. a) Relative gene expression of TIMPs1-4 by real time quantitative PCR (RT-qPCR) in WT or ΔTimp fibroblasts (mean ±
Microporous Membrane- Based Cell Culture Insert Systems Introduction and Key Applications
Microporous Membrane- Based Cell Culture Insert Systems Introduction and Key Applications Marshall Kosovsky, Ph.D. May 12, 2009 Topics for Discussion Overview of microporous membrane insert platform Membrane
CellPlayer HeLa NucLight Red
Essen BioScience Catalog Number: 4489 Storage Liquid Nitrogen Note: Cells can be thawed and cultured immediately upon receipt or stored in liquid nitrogen for long-term storage. Storage at -8 C is not
System. xcelligence System RTCA HT Instrument. Application Note No. 14 /January Long-Term High-Throughput Cytotoxicity Profiling
System Application Note No. 14 /January 2013 xcelligence System RTCA HT Instrument Long-Term High-Throughput Cytotoxicity Profiling For life science research only. Not for use in diagnostic procedures.
CytoSelect 48-Well Cell Adhesion Assay (Fibronectin-Coated, Colorimetric Format)
Product Manual CytoSelect 48-Well Cell Adhesion Assay (Fibronectin-Coated, Colorimetric Format) Catalog Number CBA-050 48 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction
SensoLyte Rh110 Cathepsin K Assay Kit *Fluorimetric* Revision#1.2 Last Updated: May 2017 Catalog # Kit Size
SensoLyte Rh110 Cathepsin K Assay Kit *Fluorimetric* Revision#1.2 Last Updated: May 2017 Catalog # 72152 Kit Size 100 Assays (96-well plate) Optimized Performance: This kit detects Cathepsin K activity.
ROS Activity Assay Kit
ROS Activity Assay Kit Catalog Number KA3841 200 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials
Fang et al. NMuMG. PyVmT unstained Anti-CCR2-PE MDA-MB MCF MCF10A
A NMuMG PyVmT 16.5+.5 47.+7.2 Fang et al. unstained Anti-CCR2-PE 4T1 Control 37.6+6.3 56.1+.65 MCF1A 16.1+3. MCF-7 3.1+5.4 MDA-M-231 42.1+5.5 unstained Secondary antibody only Anti-CCR2 SUPPLEMENTAL FIGURE
Assays for Immunology Research IncuCyte S3 Live-Cell Analysis System
ssays for Immunology Research IncuCyte S3 Live-Cell nalysis System Immune system characterization of health and disease states requires quantitative data from dynamic experimental models of the cells that
SensoLyte 520 Cathepsin K Assay Kit *Fluorimetric*
SensoLyte 520 Cathepsin K Assay Kit *Fluorimetric* Catalog # 72171 Kit Size 100 Assays (96-well plate) Optimized Performance: This kit is optimized to detect Cathepsin K activity. Enhanced Value: Ample
CytoPainter Lysosomal Staining Kit - Blue Fluorescence
ab112135 CytoPainter Lysosomal Staining Kit - Blue Fluorescence Instructions for Use For staining Lysosomes in suspension and adherent cells by using our proprietary blue fluorescence probe This product
Intracellular (Total) ROS Activity Assay Kit (Red)
Intracellular (Total) ROS Activity Assay Kit (Red) Catalog Number KA2525 200 assays Version: 04 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General
APPLICATION SPECIFIC PROTOCOL ANGIOGENESIS... 1 TABLE OF CONTENTS... 1 MONOLAYER FORMATION... 2 OPTION 1: APPLICATION OF ANGIOGENIC STIMULI...
APPLICATION SPECIFIC PROTOCOL ANGIOGENESIS AIM 3D Cell Culture Chips offer a new perspective in studying angiogenesis by allowing the growth of new vascular sprouts in a 3D matrix from a pre-existing endothelial
Supplementary Information
Supplementary Information Figure S1. Int6 gene silencing efficiency. (A) Western Blot analysis of Int6 expression at different times after sirna transfection. Int6 expression is strongly silenced in Int6
Effect of BD Matrigel Matrix Overlay and BD Matrigel Matrix Thin Coat on CYP450 Activities in Cryo Human Hepatocytes. Rongjun Zuo.
Effect of BD Matrigel Matrix Overlay and BD Matrigel Matrix Thin Coat on CYP450 Activities in Cryo Human Hepatocytes Rongjun Zuo November 10, 2010 Topics Overview of Hepatocyte Products P450 Induction
Challenges for 3D Cancer Cell Culture On Basement Membrane. AACR, 2009 Presented by Hynda K. Kleinman, PhD
Management of Disease Through DNA Repair Challenges for 3D Cancer Cell Culture On Basement Membrane AACR, 2009 Presented by Hynda K. Kleinman, PhD ams biotechnology (europe) ltd info@amsbio.com www.amsbio.com
High Content Imaging : Meaningful pictures for relevant results in dermocosmetology
High Content Imaging : Meaningful pictures for relevant results in dermocosmetology We deliver High Content screening services with exhaustive, high quality and robust phenotypic data. Nathalie MAUBON,
Fluorescence Microscopy
Fluorescence Microscopy Imaging Organelles Mitochondria Lysosomes Nuclei Endoplasmic Reticulum Plasma Membrane F-Actin AAT Bioquest Introduction: Organelle-Selective Stains Organelles are tiny, specialized
CytoSelect 24- Well Cell Invasion Assay (Basement Membrane, Fluorometric Format)
Product Manual CytoSelect 24- Well Cell Invasion Assay (Basement Membrane, Fluorometric Format) Catalog Number CBA- 111 12 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction
Plate-Based Assay Methods for the Assessment of Cellular Health
Plate-Based Assay Methods for the Assessment of Cellular Health Andrew L. Niles, Senior Research Scientist 2012, Promega Corporation. Biological Outcomes in Cell Culture Treatment -Small molecule -Bio-molecule
Focus Application. Compound-Induced Cytotoxicity
xcelligence System Real-Time Cell Analyzer Focus Application Compound-Induced Cytotoxicity For life science research only. Not for use in diagnostic procedures. Featured Study: Using the Time Resolving
Assays for Immuno-oncology Research Real-time automated measurements of immune and tumor cell dynamics within your incubator
INCUCYTE LIVE-CELL ANALYSIS SYSTEM Assays for Immuno-oncology Research Real-time automated measurements of immune and tumor cell dynamics within your incubator See what your cells are doing and when they
Figure S1: Effects on haptotaxis are independent of effects on cell velocity A)
Supplemental Figures Figure S1: Effects on haptotaxis are independent of effects on cell velocity A) Velocity of MV D7 fibroblasts expressing different GFP-tagged Ena/VASP family proteins in the haptotaxis
C ELL CANCER CELL ASSAYS. Cell Adhesion. Cell Invasion/ Cell Migration. Endothelial Cell Invasion. Tube Formation. 3-D Culture.
C ELL I N VA S I O N P RO D U C T S Cell Adhesion Cell Invasion/ Cell Migration Endothelial Cell Invasion Tube Formation Angiogenesis CANCER CELL ASSAYS 3-D Culture CA N C ER CELL AS S AYS The behavior
Focus Application. Compound-Induced Cytotoxicity
xcelligence System Real-Time Cell Analyzer Focus Application Compound-Induced Cytotoxicity Featured Study: Using the Time Resolving Function of the xcelligence System to Optimize Endpoint Viability and
Nature Protocols: doi: /nprot Supplementary Figure 1. Fluorescent titration of probe CPDSA.
Supplementary Figure 1 Fluorescent titration of probe CPDSA. Fluorescent titration of probe CPDSA (10 um) upon addition of GSH in HEPES (10 mm, ph = 7.4) containing 10% DMSO. Each spectrum was recorded
(a) Significant biological processes (upper panel) and disease biomarkers (lower panel)
Supplementary Figure 1. Functional enrichment analyses of secretomic proteins. (a) Significant biological processes (upper panel) and disease biomarkers (lower panel) 2 involved by hrab37-mediated secretory
axion Protocol Cell Culture on Microelectrode Arrays Cell Type: GE Healthcare - Cytiva TM Plus Cardiomyocytes BioSystems v. 1.0
axion BioSystems Cell Culture on Microelectrode Arrays Cell Type: GE Healthcare - Cytiva TM Plus Cardiomyocytes Protocol v. 1.0 Trademarks Axion BioSystems, Inc. and the logo are trademarks of Axion BioSystems,
AXION PLATES INDUSTRY-LEADING 768 ELECTRODES AT THE THROUGHPUT YOU NEED AXION MEA PLATES
AXION PLATES INDUSTRY-LEADING 768 ELECTRODES AT THE THROUGHPUT YOU NEED Your research requires insightful, high-quality data from microelectrode array (MEA) experiments. Axion BioSystems MEA plates meet
Cellular metabolism made easy: Microplate assays for mitochondrial function & glycolytic flux
Cellular metabolism made easy: Microplate assays for mitochondrial function & glycolytic flux Convenient Microplate Based Analysis of Cell Metabolism Resuspend Add Reagent Measure A simple push-button
SUPPLEMENTARY INFORMATION
DOI:.38/ncb3399 a b c d FSP DAPI 5mm mm 5mm 5mm e Correspond to melanoma in-situ Figure a DCT FSP- f MITF mm mm MlanaA melanoma in-situ DCT 5mm FSP- mm mm mm mm mm g melanoma in-situ MITF MlanaA mm mm
Quantitative Data Analysis Assignment Sample Newessays.co.uk
Absorbance Quantitative Data Analysis Assignment Sample Newessays.co.uk Part A: Results of the Study Is there a difference of curve profile between the MTT assay and the cell number? What do the different
HCC1937 is the HCC1937-pcDNA3 cell line, which was derived from a breast cancer with a mutation
SUPPLEMENTARY INFORMATION Materials and Methods Human cell lines and culture conditions HCC1937 is the HCC1937-pcDNA3 cell line, which was derived from a breast cancer with a mutation in exon 20 of BRCA1
Co-culturing and Assaying Spheroids in the Corning Spheroid Microplate
Co-culturing and Assaying Spheroids in the Corning Spheroid Microplate Application Note Audrey Bergeron, Hannah Gitschier Corning Incorporated, Life Sciences Kennebunk, Maine Introduction Two-dimensional
Supplementary Figure 1. Characterization of NMuMG-ErbB2 and NIC breast cancer cells expressing shrnas targeting LPP. NMuMG-ErbB2 cells (a) and NIC
Supplementary Figure 1. Characterization of NMuMG-ErbB2 and NIC breast cancer cells expressing shrnas targeting LPP. NMuMG-ErbB2 cells (a) and NIC cells (b) were engineered to stably express either a LucA-shRNA
(A) Dose response curves of HMLE_shGFP (blue circle), HMLE_shEcad (red square),
Supplementary Figures and Tables Figure S1. Validation of EMT-selective small molecules (A) Dose response curves of HMLE_shGFP (blue circle), HMLE_shEcad (red square), and HMLE_Twist (black diamond) cells
Lipid Droplets Fluorescence Assay Kit
Lipid Droplets Fluorescence Assay Kit Item No. 500001 www.caymanchem.com Customer Service 800.364.9897 Technical Support 888.526.5351 1180 E. Ellsworth Rd Ann Arbor, MI USA TABLE OF CONTENTS GENERAL INFORMATION
Essential Medium, containing 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin. Huvec were cultured in
Supplemental data Methods Cell culture media formulations A-431 and U-87 MG cells were maintained in Dulbecco s Modified Eagle s Medium. FaDu cells were cultured in Eagle's Minimum Essential Medium, containing
CytoSelect Tumor- Endothelium Adhesion Assay
Product Manual CytoSelect Tumor- Endothelium Adhesion Assay Catalog Number CBA- 215 100 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Cancer metastasis comprises several
CytoSelect 96- Well Cell Invasion Assay (Collagen I, Fluorometric Format)
Product Manual CytoSelect 96- Well Cell Invasion Assay (Collagen I, Fluorometric Format) Catalog Number CBA- 112- COL 96 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction The
Human Pluripotent Stem Cell Cardiomyocyte Differentiation Kit (PSCCDK) Introduction Kit Components Cat. # # of vials Reagent Quantity Storage
Human Pluripotent Stem Cell Cardiomyocyte Differentiation Kit (PSCCDK) Catalog #5901 Introduction Human pluripotent stem cells (hpsc), including embryonic stem cells (ESC) and induced pluripotent stem
QS S Assist KINASE_ADP-Glo TM Kit
QS S Assist KINASE_ADP-Glo TM Kit Description KINASE ADP-Glo TM kit is designed for use in biochemical kinase assays based on a Luminescent ADP Detection Assay (ADP-Glo TM ). The kit includes human kinase,
Supplementary Figure 1. SA-β-Gal positive senescent cells in various cancer tissues. Representative frozen sections of breast, thyroid, colon and
Supplementary Figure 1. SA-β-Gal positive senescent cells in various cancer tissues. Representative frozen sections of breast, thyroid, colon and stomach cancer were stained with SA-β-Gal and nuclear fast
Validation & Assay Performance Summary
Validation & Assay Performance Summary LanthaScreen IGF-1R GripTite Cells Cat. no. K1834 Modification Detected: Phosphorylation of Multiple Tyr Residues on IGF-1R LanthaScreen Cellular Assay Validation
ab Cell Invasion Assay (Basement Membrane), 24-well, 8 µm
Version 1 Last updated 29 June 2018 ab235882 Cell Invasion Assay (Basement Membrane), 24-well, 8 µm For the measurement of cell invasion in response to stimuli. This product is for research use only and
NF-κB p65 (Phospho-Thr254)
Assay Biotechnology Company www.assaybiotech.com Tel: 1-877-883-7988 Fax: 1-877-610-9758 NF-κB p65 (Phospho-Thr254) Colorimetric Cell-Based ELISA Kit Catalog #: OKAG02015 Please read the provided manual
Modulating Glucose Uptake in Skeletal Myotubes: Insulin Induction with Bioluminescent Glucose Uptake Analysis
icell Skeletal Myoblasts Application Protocol Modulating Glucose Uptake in Skeletal Myotubes: Insulin Induction with Bioluminescent Glucose Uptake Analysis Introduction The skeletal muscle is one of the
Assay Name: Transwell migration using DAPI
Assay Name: Transwell migration using DAPI Assay ID: Celigo_04_0001 Table of Contents Experiment: Identification of cells which have migrated through the membrane of a transwell insert....2 Celigo Setup...2
20X Buffer (Tube1) 96-well microplate (12 strips) 1
PROTOCOL MitoProfile Rapid Microplate Assay Kit for PDH Activity and Quantity (Combines Kit MSP18 & MSP19) 1850 Millrace Drive, Suite 3A Eugene, Oregon 97403 MSP20 Rev.1 DESCRIPTION MitoProfile Rapid Microplate
CytoPainter LysoGreen Indicator Reagent
ab176826 CytoPainter LysoGreen Indicator Reagent Instructions for Use For staining lysosomes in live cells with our proprietary Green probe. This product is for research use only and is not intended for
Supplementary Figure 1
CD31 FN Supplementary Figure 1 a Multivariate Cox regression analysis of predicting factors for disease-free and overall survival in 435 HNSCC patients b FN staining in whole sections of HNSCC c FN expression
Corning BioCoat Matrigel Invasion Chamber
Corning BioCoat Matrigel Invasion Chamber Catalog No. 354480, 354481 Guidelines for Use Discovery Labware, Inc., Two Oak Park, Bedford, MA 01730, Tel: 1.978.442.2200 (U.S.) CLSTechServ@Corning.com www.corning.com/lifesciences
XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System
Application Note Normalization XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System Authors Yoonseok Kam Kellie Chadwick Ned Jastromb Brian P. Dranka Agilent Technologies,
Supplemental information
Carcinoemryonic antigen-related cell adhesion molecule 6 (CEACAM6) promotes EGF receptor signaling of oral squamous cell carcinoma metastasis via the complex N-glycosylation y Chiang et al. Supplemental
Welcome to the Exhibitor Tutorial presented by
Welcome to the Exhibitor Tutorial presented by February 1, 217 1 Calcium Handling Assays with Human ipsc-derived Cell Types Coby Carlson, Ph.D. 7 February 217 Transformative Potential of ipsc Technology
HEK293FT cells were transiently transfected with reporters, N3-ICD construct and
Supplementary Information Luciferase reporter assay HEK293FT cells were transiently transfected with reporters, N3-ICD construct and increased amounts of wild type or kinase inactive EGFR. Transfections
Pluricyte Cardiomyocytes
Pluricyte Cardiomyocytes Manual Version 2.1 / March 2018 Contents 1. Introduction 2 2. Equipment, Materials and Reagents 3 3. Methods 4 3.1 Coating of tissue culture plates 4 3.2 Thawing Pluricyte Cardiomyocytes
Glucose Uptake-Glo Assay
TECHNICAL MANUAL Glucose Uptake-Glo Assay Instructions for Use of Products J1341, J1342, and J1343 Revised 2/17 TM467 Glucose Uptake-Glo Assay All technical literature is available at: www.promega.com/protocols/
SensoLyte 490 HIV-1 Protease Assay Kit *Fluorimetric*
SensoLyte 490 HIV-1 Protease Assay Kit *Fluorimetric* Catalog # 71127 Unit Size Kit Size 1 kit 500 assays (96-well) or 1250 assays (384-well) This kit is optimized to detect the activity of human immunodeficiency
ab HIV-1 Protease Inhibitor Screening Kit (Fluorometric)
Version 1 Last updated 2 March 2017 ab211106 HIV-1 Protease Inhibitor Screening Kit (Fluorometric) For the rapid, sensitive and accurate screening of potential HIV-1 Protease inhibitors. This product is
Evaluation of directed and random motility in microslides Assessment of leukocyte adhesion in flow chambers
Evaluation of directed and random motility in microslides Motility experiments in IBIDI microslides, image acquisition and processing were performed as described. PMN, which ended up in an angle < 180
Human Urokinase / PLAU / UPA ELISA Pair Set
Human Urokinase / PLAU / UPA ELISA Pair Set Catalog Number : SEK10815 To achieve the best assay results, this manual must be read carefully before using this product and the assay is run as summarized
Supplementary Figure (OH) 22 nanoparticles did not affect cell viability and apoposis. MDA-MB-231, MCF-7, MCF-10A and BT549 cells were
Supplementary Figure 1. Gd@C 82 (OH) 22 nanoparticles did not affect cell viability and apoposis. MDA-MB-231, MCF-7, MCF-10A and BT549 cells were treated with PBS, Gd@C 82 (OH) 22, C 60 (OH) 22 or GdCl
Product # R8132 (Explorer Kit) R8133 (Bulk Kit)
Product Insert QBT Fatty Acid Uptake Assay Kit Product # R8132 (Explorer Kit) R8133 (Bulk Kit) Introduction About the Fatty Acid Uptake Assay Kit The homogeneous QBT Fatty Acid Uptake Assay Kit from Molecular
Trevigen s Extracellular Matrix Proteins for Triculture of Breast Cancer Spheroids, Endothelial Tubules, and Human Mesenchymal Stem Cells
Trevigen s Extracellular Matrix Proteins for Triculture of Breast Cancer Spheroids, Endothelial Tubules, and Human Mesenchymal Stem Cells Gabriel Benton, Ph.D. and Jay George, Ph.D., Trevigen, Inc. Gaithersburg,
ab Lipid Peroxidation (MDA) Assay kit (Colorimetric/ Fluorometric)
Version 10b Last updated 19 December 2018 ab118970 Lipid Peroxidation (MDA) Assay kit (Colorimetric/ Fluorometric) For the measurement of Lipid Peroxidation in plasma, cell culture and tissue extracts.
Electronic Supplementary Information
Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry Please do 2017 not adjust margins Nanoscale Electronic Supplementary Information Mono-fullerenols modulating
Performing Bioenergetic Analysis: Seahorse XFe96 Analyzer
icell Cardiomyocytes 2 Application Protocol Performing Bioenergetic Analysis: Seahorse XFe96 Analyzer Introduction The myocardium is the most metabolically active tissue in the body and is highly sensitive
TFEB-mediated increase in peripheral lysosomes regulates. Store Operated Calcium Entry
TFEB-mediated increase in peripheral lysosomes regulates Store Operated Calcium Entry Luigi Sbano, Massimo Bonora, Saverio Marchi, Federica Baldassari, Diego L. Medina, Andrea Ballabio, Carlotta Giorgi
DEVELOPMENTAL VALIDATION OF SPERM HY-LITER EXPRESS Jennifer Old, Chris Martersteck, Anna Kalinina, Independent Forensics, Lombard IL
DEVELOPMENTAL VALIDATION OF SPERM HY-LITER EXPRESS Jennifer Old, Chris Martersteck, Anna Kalinina, Independent Forensics, Lombard IL Background and Introduction SPERM HY-LITER is the first immunofluorescent
SUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION Advances in pancreatic islet monolayer culture on glass surfaces enable superresolution microscopy and insights into beta cell ciliogenesis and proliferation Edward A. Phelps,
Annexin V-PE Apoptosis Detection Kit
Annexin V-PE Apoptosis Detection Kit Catalog Number KA0716 100 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information...
MTS assay in A549 cells
Project: VIGO MTS assay in A549 cells Detection of cell viability/activity AUTHORED BY: DATE: Cordula Hirsch 20.01.2014 REVIEWED BY: DATE: Harald Krug 10.04.2014 APPROVED BY: DATE: DOCUMENT HISTORY Effective
PhosFree TM Phosphate Assay Biochem Kit
PhosFree TM Phosphate Assay Biochem Kit (Cat. # BK050) ORDERING INFORMATION To order by phone: (303) - 322-2254 To order by Fax: (303) - 322-2257 To order by e-mail: cservice@cytoskeleton.com Technical
Assay Kit for Measurement of Proteoglycan. (Sulfated Glycosaminoglycan Quantification Kit)
Assay Kit for Measurement of Proteoglycan. (Sulfated Glycosaminoglycan Quantification Kit) Cat. No. 280560-N INTRODUCTION Glycosaminoglycans (GAGs) are a major component of the extracellular matrix (ECM)
A Precise Bicoid Gradient is Nonessential During Cycles for Precise Patterning in the Drosophila Blastoderm
Supporting Information for A Precise Bicoid Gradient is Nonessential During Cycles 11-13 for Precise Patterning in the Drosophila Blastoderm Elena M. Lucchetta, Meghan E. Vincent and Rustem F. Ismagilov*
Protein Arrays High Throughput Protein Quantification from Total Cell Lysates
Protein Arrays High Throughput Protein Quantification from Total Cell Lysates Dr. Markus Ehrat Mr. Markus Tobler Zeptosens A Division of Bayer (Schweiz) AG Benkenstr. 254 CH-4108 Witterswil Switzerland
CD34 + VEGFR-3 + progenitor cells have a potential to differentiate towards lymphatic endothelial cells
CD34 + VEGFR-3 + progenitor cells have a potential to differentiate towards lymphatic endothelial cells Tan YZ et al. J Cell Mol Med. (2014 Mar;18(3):422-33) Denise Traxler-Weidenauer April 2014 Introduction
Supplementary Data Table of Contents:
Supplementary Data Table of Contents: - Supplementary Methods - Supplementary Figures S1(A-B) - Supplementary Figures S2 (A-B) - Supplementary Figures S3 - Supplementary Figures S4(A-B) - Supplementary
Supplementary Material
Electronic Supplementary Material (ESI) for Integrative Biology. This journal is The Royal Society of Chemistry 2018 Supplementary Material 1 Supplemental Figures Supplemental Figure S1. Mechanical properties
Measuring Cardiac Activity:
icell Cardiomyocytes 2 Application Protocol Measuring Cardiac Activity: Impedance and Extracellular Field Potential Detection with CardioExcyte 96 System Introduction icell Cardiomyocytes 2, human cardiomyocytes
Supplementary Data Dll4-containing exosomes induce capillary sprout retraction ina 3D microenvironment
Supplementary Data Dll4-containing exosomes induce capillary sprout retraction ina 3D microenvironment Soheila Sharghi-Namini 1, Evan Tan 1,2, Lee-Ling Sharon Ong 1, Ruowen Ge 2 * and H. Harry Asada 1,3
Optimization of a LanthaScreen Kinase assay for NTRK1 (TRKA)
Optimization of a LanthaScreen Kinase assay for NTRK1 (TRKA) Overview This protocol describes how to develop a LanthaScreen kinase assay designed to detect and characterize kinase inhibitors. The development
Sequence Coverage (%) Profilin-1 P UD 2
Protein Name Accession Number (Swissprot) Sequence Coverage (%) No. of MS/MS Queries Mascot Score 1 Reference Cytoskeletal proteins Beta-actin P60709 37 14 298 Alpha-actin P68032 33 10 141 20 Beta-actin-like
Supplementary Figure 1
Supplementary Figure 1 6 HE-50 HE-116 E-1 HE-108 Supplementary Figure 1. Targeted drug response curves of endometrial cancer cells. Endometrial cancer cell lines were incubated with serial dilutions of
Instructions. Fuse-It-mRNA easy. Shipping and Storage. Overview. Kit Contents. Specifications. Note: Important Guidelines
Membrane fusion is a highly efficient method for transfecting various molecules and particles into mammalian cells, even into sensitive and primary cells. The Fuse-It reagents are cargo-specific liposomal
CyBio Felix Your Automated Application Starts Here
Title Label Lab Automation CyBio Felix Your Automated Application Starts Here 2 CyBio FeliX Future-Proof Liquid Handling Automation Transform your manual workflows to automated processes on your benchtop!
Supplementary Figure 1. Basal level EGFR across a panel of ESCC lines. Immunoblots demonstrate the expression of phosphorylated and total EGFR as
Supplementary Figure 1. Basal level EGFR across a panel of ESCC lines. Immunoblots demonstrate the expression of phosphorylated and total EGFR as well as their downstream effectors across a panel of ESCC
CELL-BASED ASSAYS. bionova científica, s.l. Tel.: Fax:
CELL-BASED ASSAYS Colony Formation / Cell Transformation Cell Adhesion Cell Migration / Invasion Phagocytosis Cell Viability / Death Senescence Cell Contraction Angiogenesis Autophagy 1 5 7 16 17 18 18