CD44 glycoproteins in colorectal cancer; expression, function and prognostic value Wielenga, V.J.M.

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1 UvA-DARE (Digital Academic Repository) CD44 glycoproteins in colorectal cancer; expression, function and prognostic value Wielenga, V.J.M. Link to publication Citation for published version (APA): Wielenga, V. J. M. (1999). CD44 glycoproteins in colorectal cancer; expression, function and prognostic value General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. UvA-DARE is a service provided by the library of the University of Amsterdam ( Download date: 21 Nov 2017

2 Chapter 4 CD44 SPLICE VARIANTS AS PROGNOSTIC MARKERS IN COLORECTAL CANCER. Vera J.M. Wielenga, Robbert van der Voort, Jan-Willem R. Mulder, Philip M. Kruyt, Wiebo F. Weidema, Johan Oosting, Cees A. Seldenrijk, Cees van Krimpen, G. Johan A. Offerhaus, Steven T. Pais Scan J Gastroenterol 1998, 33:

3 CD44 Splice Variants as Prognostic Markers in Colorectal Cancer V. J. M. WIELENGA, R. VAN DER VOORT, J. W. R. MULDER, P. M. KRUYT, W. F. WEIDEMA, J. OUSTING, C. A. SELDENR1JK, C. VAN KRIMPEN, G. J. A. OFFERHAUS & S. T. PALS Depts. of Pathology, Surgery, and Clinical Epidemiology and Biostatistics, Academic Medical Center, University of Amsterdam, Amsterdam, and Depts. of Surgery and Pathology, Reinier de Graaf Hospital, Delft, The Netherlands Wielenga VJM, van der Voort R, Mulder JWR, Kruyt PM, Weidema WF, Oosting J, Seldenrijk CA, van Krimpen C, Offerhaus GJA, Pals ST. CD44 splice variants as prognostic markers in colorectal cancer. Scand J Gastroenterol 1998;33: Background: Splice variants of CD44 play a causal role in the metastatic spread of pancreatic carcinoma in the rat. In previous studies we have shown that homologues of these CD44 isoforms (CD44v6) are overexpressed during colorectal tumorigenesis in man and that CD44v6 overexpression is associated with an unfavorable prognosis in this disease. In the present study we have assessed the prognostic significance of CD44 variants containing exon v5. In addition, we have used a panel of different antibodies against CD44v6 and applied a combined scoring system to improve its value as prognosticator. Methods: Expression of CD44 variants was studied by immunohistochemistry on frozen tissue sections, and the prognostic value of the CD44 variant expression was assessed using univariate and multivariate analysis. Results: Our studies show that expression of CD44v6, but not CD44v5, has significant prognostic value. Analysis of CD44v6 expression by means of a combined scoring system, on the basis of a panel of three different monoclonal antibodies (mabs), makes CD44v6 a highly significant prognostic marker that is independent of Dukes stage, tumor grade, or tumor localization. Conclusion: Assessment of CD44v6 expression by a combination of mabs yields an independent prognosticator that may be of value in identifying patients with a high propensity to develop distant metastasis. Key words: CD44; cell adhesion; colorectal cancer; metastasis; prognosis S. T. Pals, M.D., Ph.D., Dept. of Pathology, Academic Medical Centre, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands (fax: ) Tumor metastasis is the principal cause of death in cancer patients. Therefore, effective assessment of the metastatic potential of tumors is a prime goal for cancer research (1). In patients with colorectal cancer approximately half of the carcinomas that appear to be radically excised at surgery (Dukes B and C) will eventually give rise to progressive disease due to the presence of occult metastases at the time of operation. Markers that can predict metastasis in these tumor stages are of great potential value for the clinician, since they identify patients who are likely to benefit from adjuvant chemotherapy and/or radiotherapy. Several recent studies suggest that CD44 splice variants might be such markers. CD44 is a widely distributed family of cell surface glycoproteins which has been implicated in several important biologic processes including hematopoiesis (2, 3), lymphocyte activation (2,4, 5, 6), lymphocyte homing (2, 7), and tumor progression and metastasis (2, 8-19). Due to alternative splicing of at least loexons (vl-vlo), which encode domains of the extracellular portion of the molecule, a large number of CD44 splice variants is generated (11,20). Posttranslational modification, which includes N- and O-linked glycosylation and decoration with glycosaminoglycan (GAG) side chains, adds further diversity to CD44 (2,21,22). The finding that CD44 variants, containing sequences encoded by exon v6. confer full metastatic potential to rat carcinoma cell lines (11) led to the hypothesis that homologous CD44 variants might also mediate metastasis formation in humans. We have previously shown that CD44 splice variants containing exon v5 and v6, which are hardly or not at all expressed in the normal epithelium of the colon and rectum (23,24), are increasingly overexpressed during colorectal tumorigenesis (14, 15). Furthermore, we found evidence of an association between overexpression of CD44v6-containing splice variants and risk of tumor-related death (16). In the present study we have extended these observations by exploring the prognostic significance of CD44 variants containing exon v5. Furthermore, we show that assessment of CD44v6 expression by a panel of monoclonal antibodies (mabs) makes CD44v6 a highly significant marker for metastatic propensity in colorectal cancer, independent of conventional prognosticators. MATERIALS AND METHODS Tissue samples The study set consisted of 68 primary colorectal carcinomas, removed at operation between 1 January 1983 and 1 January at the Dept. of Surgery, Reinier de Graaf 60

4 B v2 v3 v4 v5 v6 v7 v8 v9 [ vlo S VFF7 Fig. IA. Schematic representation of the CD44 gene. Open boxes indicate exons that can be alternatively spliced. TM = transmembrane region. 1B. Schematic representation of the CD44 protein with localizations of the epitopes that are recognized by the monoclonal antibodies VFF8, VFF4, VFF7, and VFF18. Anti-variant antibodies were raised against a bacterially expressed fusion protein encoded by pgex CD44v HPKII (v3-vl0). v2-10 are domains encoded by variant exons. Hospital, Delft, for which snap-frozen tissue and follow-up until 1 June 1992 ( years) was available. The mean age of the patients at diagnosis was 69.7 years (range, years), and the male to female ratio was 28:40. The tumors were staged in accordance with a modification of the original Dukes classification (25). The four Dukes classes are defined as follows: Dukes A, disease limited to the bowel wall; Dukes B, extension through the deep muscle without metastases; Dukes C, regional lymph-node metastases but radical resection; Dukes D, distant metastases. The tumors were graded as well-differentiated, moderately differentiated, or poorly differentiated, using standard criteria (26). Right-sided tumors came from the cecum, ascending colon, and transverse colon, and left-sided tumors from the descending colon, sigmoid, and rectum. Detection of CD44 variants Frozen tissue sections were tested for the expression of CD44 splice variants by immunohistochemistry as described previously (6, 15), using a panel of mabs raised against a bacterially expressed fusion protein encoded by exons v3~ vlo. Tonsillar tissue was used as a positive control in each staining, and isotype (IgGl)-matched mabs were used as negative controls. The mabs used were VFF4, VFF7, and VFF18, which recognize CD44v6 (Bender Co., Vienna, Austria), and VFF8, which recognizes CD44v5 (Bender Co.) (Fig. 1). A detailed description of these antibodies and their specificities has been published elsewhere (6, 23). The anti-cd44v6 mab VFF18 recognizes the epitope QWFGNRWHEGYRQT on exon v6 (23). The other two anti-cd44v6 mabs compete with VFF18 and with each other for binding, hence their epitopes are identical or overlapping. The relative affinities of VFF18, VFF4, and VFF7, as determined by BlAcore using as immobilized antigen the 61 bacterial GST fusion protein CD44v3-10, were 100%, 71%, and 15%, respectively (27). All slides were read by four independent observers (J. W. Mulder, C. A. Seldenrijk, G. J. A. Offerhaus, S. T.Pals), and discrepancies were solved by consensus. During the whole procedure the observers were blinded for disease outcome. On the basis of the estimated percentage of the positively staining tumor cells, the stained samples were scored as follows: 0 (low/negative) = less than 10% of the cells positive; 1 (intermediate) = 10% to 50% of the cells positive; and 2 (high) = more than 50% of the cells positive. For the three mabs that recognize exon v6 the scores of each patient were added. Hence, the possible 'cumulative score' for exon v6 expression for each patient ranges from 0 to 6. For statistical analysis patients were grouped into three categories: CD44v6 low = 0-2; CD44v6 intermediate = 3 and 4;CD44v6high = 5 and 6. Statistical analysis In univariate analysis survival functions were estimated with the Kaplan-Meier method. Comparison of survivor functions between groups was performed with the log-rank test. Multivariate analysis by means of the Cox regression model was executed with the expression of exon v6 'cumulative score', tumor localization, tumor stage, and tumor Table I. Percentage of colorectal carcinomas with low, intermediate, and high staining for CD44 variants Expression level Low Intermediate High VFF8 (v5), n = 68 18% 29% 53% VFF7 (v6), n = 68 81% 12% 7% VFF4 (v6), n = 68 31% 31% 38% VFF18(v6), n = 68 6% 21% 73% Low, intermediate, and high: expression on <10%, 10-50%, and >50% of the tumor cells, respectively.

5 Table II. Prognostic significance of tumor localization, Dukes stage, tumor grade, and CD44 variant expression Univariate analysis No. of patients Median survival (days) P valuet i'"'l. Ui ' "- EXON V6 (VFF4) Tumor localization Right colon Left colon Tumor stage Dukes B < Dukes C Dukes D 9 59 Tumor grade Well differentiated Moderately differentiated Poorly differentiated Exon v6* Low Intermediate High Exon v5 Low Intermediate High * Cumulative score after staining with monoclonal antibodies VFF4, VFF7, and VFF18 (see Materials and Methods). t Log-rank test. grade as variables. With the aid of the stepwise backward regression technique, the most parsimonious model was achieved. The likelihood ratio test was used to test for significance. The P values of the variables were calculated by eliminating each variable. RESULTS Expression of CD44 variants in colorectal cancer The expression of CD44 splice variants in the study group of 68 colorectal carcinomas is shown in Table I. Each of the variants examined was expressed at levels that varied greatly between individual tumors: whereas some tumors were completely negative for a given variant, other tumors expressed the same variant strongly on all or virtually all of the tumor cells. Positivity (intermediate or high expression) of CD44v5 was present in 82%. For CD44v6 the percentage of positive tumors varied greatly depending on the mab used for the detection of v6: With mabs VFF7, VFF4, and VFF18, CD44v6 expression was found in 19%, 69%, and 94% of the carcinomas, respectively (Table I). These differences in the percentage of positive tumors corresponded consistently with differences in antibody affinity (VFF18 > VFF4 > VFF7). Nevertheless, the staining patterns obtained with the three different mabs were identical. CD44 variants and survival To test the hypothesis that CD44 splice variant expression plays a role in the dissemination and prognosis of colorectal TIME (YEARS) Fig. 2. Kaplan-Meier curves showing the relation between Dukes stage, CD44v5 and v6 expression, and survival in patients with colorectal carcinoma, measured with the individual monoclonal antibodies: VFF8 </> = 0.5), VFF4 (/» = 0.004), VFF7 (/» = 0.002), and VFF18 (P = 0.01). Dotted line = low expression; broken line = intermediate expression; unbroken line = high expression; + = censored cases. The upper right diagram shows the relation between Dukes stage and survival. Dotted line = Dukes B; broken line = Dukes C; unbroken line = Dukes D; + = censored cases. cancer, we assessed the relation between CD44v5 and CD44v6 expression and patient survival in our study group (Table O, Fig. 2). Additional risk factors considered in the study were primary tumor localization (right- versus leftsided), Dukes stage, and tumor grade. Univariate analysis showed that CD44v6 but not CD44v5 expression was correlated with survival. For CD44v6 a significant correlation was found with all three mabs used. Fig. 3 shows the cumulative score for CD44v6, obtained by integrating the scores from the three separate mabs. In addition to CD44v6, Dukes stage (Table II, Fig. 2) and tumor grade (Table O) were also correlated with tumor-related death. CD44v6 expression was significantly correlated with Dukes stage (chi-square, P = 0.04), and there was no significant correlation between CD44v6 expression and tumor grade (chi-square, P = 0.3). CD44 splice variants as prognostic parameter To explore whether CD44 splice variant expression has an 62

6 "I t+"h TIME (YEARS) Fig. 3. Kaplan-Meier curve showing the relation between CD44v6 expression and survival after integrating the scores of the three separate monoclonal antibodies (VFF7, VFF4, and VFF18) against CD44v6 (P = ). independent prognostic value, multivariate analysis was performed with the CD44v6 cumulative score, tumor stage, tumor grade, and tumor localization as variables (Table III). In tumor stage only patients with Dukes B and C tumors were included, representing the patients who underwent radical surgery. After stepwise backward regression, Dukes stage and CD44v6 expression were the determining factors in the assessment of prognosis (Table Til). In Fig. 4 the final effect of exon v6 is shown versus an average Dukes classification, visualizing the clinical impact of CD44v6 expression on survival. DISCUSSION Prediction of disease outcome in colorectal cancer is currently based mainly on the stage of tumor growth at the time of resection. However, patients with tumors of the same stage often show a diametrically different outcome. Hence, additional independent prognostic markers are needed to provide a rationale to adjust therapeutic approaches. In our prr.ent study we provide evidence that CD44 splice variants have this potential. Using the same patient group for our study material, we had previously found that expression of CD44v6 on the resected primary tumor is associated with an unfavorable prognosis in colorectal carcinoma (16). In this study we have further explored and improved the value of CD44v6 as a prognosticator in colorectal cancer, by assessing CD44v6 expression with a panel of three mabs, which recognize identical or overlapping epitopes on CD44v6 but differ in affinity (23, 27). This affinity difference was reflected by the sensitivity with which CD44v6 was detected and resulted in a vastly different number of tumors stained positive with each of the antibodies (Table I). A significant correlation between CD44v6 expression and survival was observed with each of the individual mabs (Fig. 2). However, by integrating the results of the mab panel to a cumulative CD44v6 score (Fig. 3), which represents a semiquantitative assessement of CD44v6 expression, a more optimal differentiation between risk groups was obtained. In multivariate analysis of Dukes B and C tumor patients the patient group that underwent radical surgery and hence might benefit from adjuvant therapy for occult metastatic disease, this cumulative CD44v6 score emerged as a highly significant independent prognostic factor (Table in. Fig. 4). The correlation of CD44v5 expression with patient survival was not statistically significant but showed the same trend as CD44v6 (Table II, Fig. 2). Several studies addressing the expression of CD44 splice variants in colorectal cancer have recently been published (12-16,28-33). They indicate that CD44 splice variants Table III. Independent prognostic significance of tumor stage, tumor grade, tumor localization, and CD44v6 expression Full model After backwards stepwise regression Hazard ratio Hazard ratio Standard (exponent Standard (exponent Multivariate analysis p 1 -coefficient error coefficient) P value ^-coefficient error coefficient) P value Tumor stage Dukes B Dukes C Tumor grade Well-differentiated Moderately differentiated Poorly differentiated Tumor localization Left colon Right colon Exon v6* Low High * Cumulative score after staining with monoclonal antibodies VFF4, VFF7, and VFF18 (see Materials and Methods). 63

7 * 0.8 fc~. X v;--. > 0.6 v ' 2 t, < = 0.4 U TIME (YEARS) Fig. 4. Impact of CD44v6 expression on survival in Dukes B and C patients. Dotted line = low expression; broken line = intermediate expression; unbroken line = high expression (P = 0.02). become overexpressed during colorectal tumorigenesis. This overexpression presumably represents an early event in the adenoma-carcinoma sequence, since it is already detectable in adenomas (14,15,28-30,32,33), where it may 1 occur before K-ras and p53 gene mutation (30). In each tumor a complex splice pattern with several different splice variants containing various combinations of exon products encoded by v2-vl0 is present (12-16, 28,29,33). The precise composition of these different variants and their functions in tumorigenesis are still unkown. Abnormal expression of variant exons was also recently shown in exfoliated colorectal cancer cells, suggesting that it may be helpful in the early, noninvasive detection of colorectal cancer (34). In conclusion, our current findings provide further evidence of a role of CD44 splice variants in colorectal tumorigenesis and of their utility as prognostic markers. Specifically, the data show that immunohistochemical assessement of CD44 splice variant expression with a panel of three mabs against CD44v6, which could easily be performed on a routine basis, yields an independent prognosticator that might be used to identify patients with a high propensity to develop distant metastasis. These patients might benefit from adjuvant chemotherapy and/or radiotherapy. ACKNOWLEDGEMENTS We thank Dr. G. R. Adolf and Dr. E. Patzelt for monoclonal antibodies VFF4, VFF7, VFF8, and VFF18 and Mr. A. Pronk for expert technical assistance. REFERENCES 1. NIH Consensus Meeting. Adjuvant therapy for patients with colon and rectal cancer. JAMA 1990;264: Lesley J, Hyman R, Kincade PW. CD44 and its interactions with the extracellular matrix. Adv Immunol 1993;4: Miyake K, Medina KL, Hayashi S, Ono S, Hamaoka T, Kincade PW. Monoclonal antibodies to Pgp-1/CD44 block lymphohemopoiesis in long-term bone marrow cultures. J Exp Med 1990;171: Denning SM, Le PT, Singer KH, Haynes BF. Antibodies against CD44, p80, lymfocyte homing receptor molecule augment peripheral blood T cell activation. J Immunol 1990;144: Shimizu Y, van Seventer GA, Siraganian R, Wahl L, Shaw S. Dual role of the CD44 molecule in T cell adhesion and activation. J Immunol 1989;143: Koopman G, Heider KH, Horst E, Adolf GR, van den Berg F. Ponta H, et al. Activated human lymphocytes and aggressive non-hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44. J Exp Med Jalkanen S, Bargatze RF, Herron LR, Butcher EC. A lymphoid cell surface glycoprotein involved in endothelial cell recognition and lymphocyte homing in man. Eur J Immunol 1986; 16: Horst E, Meijer CJLM, Radaszkiewicz T, Ossekoppele GJ, van Krieken JHJM, Pals ST. Adhesion molecules in the prognosis of diffuse large-cell lymphoma: expression of a lymphocyte homing receptor (CD44), LFA-1 (CDlla/18), and ICAM (CD54). Leukemia 1990;4: Jalkanen S, Joensuu H, Söderström KO, KJemi P. Lymphocyte homing and clinical behaviour of non-hodgkin's lymphoma. J Clin Invest 1991;87:1835^K>. 10. S y MS, Guo YJ, Stamenkovic I. Inhibition of tumor growth in vivo with a soluble CD44-immunoglobulin fusion protein. J Exp Med 1992;176: ll.gunthert U, Hofmann M, Rudy W, Reber S, Zöller M, Haußmann I, et al. A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cell lines. Cell 1991;65: Matsumara Y, Tarin D. Significance of CD44 gene products for cancer diagnosis and disease evaluation. Lancet 1992:340: Tanabe KK, Ellis LM, Saya H. Expression of CD44R1 adhesion molecule in colon carcinomas and metastases. Lancet 1993;341: Heider KH, Hofmann M, Horst E, van den Berg F, Ponta H, Herrlich P, et al. A human homologue of the rat metastasisassociated variant of CD44 is expressed in colorectal carcinomas and adenomatous polyps. J Cell Biol 1993;120: Wielenga VJM, Heider KH, Offerhaus GJA, Adolf GR. van den Berg F, Ponta H, et al. Expression of CD44 variant proteins in human colorectal cancer is related to tumor progression. Cancer Res 1993;53: Mulder JWR, Kruyt PM, Sewnath M, Oosting J, Seldenrijk CA, Weidema WF, et al. Colorectal cancer prognosis and expression of exon-v6 containing CD44 proteins. Lancet 1995;344: Kauffmann M, Heider KH, Sinn HP, Minckwitz von G, Ponta H, Herrlich P. CD44 variant exon epitopes in primary breast cancer and length of survival. Lancet 1995;345: Cooper DL, Dougherty GJ. To mestastasize or not? Selection of CD44 splice sites. Nature Med 1995;1: Stauder R, Eisterer W, Thaler J, Gunthert U. CD44 variant isoforms in non-hodgkin's lymphomas: a new independent prognostic factor. Blood 1995;85: Screaton GR, Bell MV, Jackson DG, Comelis FB, Gerth K, Bell JL. Genomic structure of DNA encoding the lymphocyte homing receptor CD44 reveals at least 12 alternatively spliced exons. Proc Natl Acad Sei USA 1992;89: Stamenkovic I, Amiot M, Pesando JM, Seed B. A lymphocyte molecule implicated in lymph node homing is a member of the cartilage link protein family. Cell 1989;56: Jackson D, Bell JI, Dickinson R, Timans J, Shields J, Whittle N. Proteoglycan forms of of the lymphocyte homing receptor CD44 are alternatively spliced variants containing the v3 exon. J Cell Biol 1995:128: Heider KH, Mulder JWR, Ostermann E, Susani E, Patzelt E, 64

8 Pals ST, et al. Splice variants of the cell surface glycoprotein CD44 associated with metastatic tumor cells are expressed in normal tissues of humans and cynomolgus monkeys. Eur J Cancer 1995;31A: Fox S, Fawcett J, Jackson DGJ, Collins I, Gatter KC. Harris AL, et a!. Normal human tissues, in addition to some tumors, express multiple different CD44 isoforms. Cancer Res 1994;54: Dukes CE. The classification of cancer of the rectum. J Pathol Bact 1932;25: Offerhaus GJA, Giardiello FM, Bruijn JA, Stijnen T, Molyvas EN, Fleuren GJ. The value of collagen IV immunohistochemistry in colorectal cancer. Cancer 1991;67: Heider KH, Sproll M, Susani S, Patzelt E, Beaumier P, Ostermann E, et al. Characterization of a high-affinity monoclonal antibody specific for CD44v6 as candidate for immunotherapy of squamous cell carcinomas. Cancer Immunol Immunother 1996;43: Orzechowski HD, Beckenbach C, Herbst H, Stolzel U, Riecken EO, Stallmach A. Expression of CD44v6 is associated with cellular dysplasia in colorectal epithelial cells. Eur J Cancer 1995;31A: Imazeki F, Yokosuka O, Yamagichi T, Ohto M. Isono K, Omata M. Expression of variant CD44-messenger RNA in colorectal adenocarcinomas and adenomatous polyps in humans. Gastroenterology 1995;110: Kim H, Yang XL, Rosada C, Hamilton SR, August JT. CD44 expression in colorectal adenomas is an early event occuring prior to K-ras and p53 gene mutation. Arch Biochem Biophys 1994;310: Rodriguez C, Monges G, Rouanet P, Dutrillaux B, Lefrançois D, Theillet C. CD44 expression patterns in breast and colon tumors: a PCR-based study of splice variants. Int J Cancer 1995;64: Jackson PA. Green MA, Pouli A. Hubberd R, Marks CG, Cook MG. Relation between stage, grade, proliferation, and expression of p53 and CD44 in adenomas and carcinomas of the colorectum. J Clin Pathol 1995;48: Gotley DC, Fawcett J, Walsh MD, Reeder JA, Simmons DL, Antalis TM. Alternatively spliced variants of the cell adhesion molecule CD44 and tumor progression in colorectal cancer. Br J Cancer 1996;74: Yoshida K, Sugino T, Bolodeoku J, Warren BF, Goodison S. Woodman A, et al. Detection of exfoliated carcinoma cells in colonic luminal washings by identification of deranged patterns of expression of the CD44 gene. J Clin Pathol 1996;49: Received 19 June 1997 Accepted 17 October

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