Viral and Host Factors in Vulvar Disease DR MICHELLE ETHERSON 26 TH OF APRIL 2016
|
|
- Doreen Wheeler
- 6 years ago
- Views:
Transcription
1 Viral and Host Factors in Vulvar Disease DR MICHELLE ETHERSON 26 TH OF APRIL 2016
2 Human Papillomavirus (HPV) Small non-enveloped Double stranded DNA Virus Genome 8000 basepairs 8 coding genes, Early (E) and Late (L) and a noncoding long control region (LCR) Doorbar et al (2005)
3 HPV Classification Similarity between the DNA sequence at each virus s most conserved region of genome, L1 Open Reading Frame (ORF) devilliers et al 2004
4 HPV Risk of Malignancy Alpha-papillomavirus most important in anogenital disease Cervical, Anal, Penile, Vaginal, Vulvar and their preinvasive intraepithelial stages Different HPV types confer different oncogenic risk Low Risk types include HPV 6 and 11 High Risk types include HPV 16, 18, 31, 33 HPV 16 provides the greatest carcinogenic risk
5 HPV 16 Major variant lineages A(1-4) = European(E) and Asian(As) lineages B(1-2) = African-1(Af-1) lineages C = African-2(Af-2) lineages D(1-3) = Asian-American(AA) lineages Cervical disease - variable risk of persistence and oncogenesis Non-European lineages (B/C/D) more pathogenic Increased risk of persistence - T350G (within E6 gene) in European lineages Burk et al (2013)
6 Vulvar Intraepithelial Neoplasia (VIN) and Vulvar Cancer (VSCC) Constructed using ISD data from Statistics/Female-Genital-Organ/#vulva ICD-51 Vulvar Cancer
7 Preti et al (2014)
8 Role of HPV in VIN and VSCC Worldwide prevalence of HPV in vulvar disease 80% in VIN 40% in VSCC HPV 16 predominates Risk of progression between 9-16% in untreated and 3% in treated higher risk in dvin Recurrence - one third of patients Treatment morbid Prophylactic Vaccination
9 Could host genetic variation contribute?
10 Single Nucleotide Polymorphisms (SNPs) Single base pair change in a genetic sequence present in at least 1% of the population SNPs can affect gene regulation and protein expression Infectious diseases and response to treatment, cancer susceptibility and prognosis
11 CXCR1 Work within our Group Association of CXCR1 SNP with CIN Interleukin-8 Receptor A Chemotaxis Tumorigenesis IL-8 + CXCR1 increased in cancer including cervical Disease progression
12 CXCR1 SNP rs Non-synonymous SNP G C Serine May to Threonine amino acid at position 276 alter structure and binding
13 CXCR1 SNP Associations Increased risk:- Asymptomatic bacteriuria in women and acute pyelonephritis in children Mucosal Leishmaniasis Active Hepatitis B and Cirrhosis Lung cancer and Gallbladder cancer Poorer response to bevacizumab and oxaliplatin-based chemotherapy in metastatic colon cancer
14 Specific Research Questions Identify viral and genetic factors contributing to VIN and VSCC development and progression This will provide valuable information for both primary prevention and improving clinical management What is the prevalence of HPV genotypes and HPV 16 variants in VIN and VSCC samples? Is the CXCR1 SNP rs associated with VIN and VSCC?? Is the CXCR1 SNP rs functional?
15 HPV 16 Variants Analysis of HPV genotypes and HPV 16 variants Collaborative work with Katie Wakeham 240 extracts of nucleic acids samples from FFPE VIN and VSCC cases HPV Genotyping via luminex based assay Clinical database produced
16 VIN Cases VSCC Cases Average Age (all) Average Age (HPV related) Total Number % HPV Positive 78.3% (130) 34.8% (23) % HPV 16 Mono-Infection 81.5% (106) 91.3% (21) % Other or Mixed Infection 18.5% 8.7%
17 VIN HPV HPV 33 9 HPV 18 3 HPV 42 2 HPV 45 1 HPV 16, 33 2 HPV 16, 45 1 HPV 16, 18 1 HPV 6, 18 1 HPV 16, 42 1 HPV 16, 45 1 HPV 16, 33, 53 1 HPV 6, 11, 16 1 VSCC HPV HPV 31 1 HPV 16, 42 1
18 Variant Analysis Sanger sequencing following PCR amplification of 2 regions within E6 Swan et al 2006
19 Primer set 1 E6(75) Forward and E6(206) Reverse 1000 bp 500 bp 200 bp Expected product size bp 1000 bp 500 bp 200 bp S i H a H e L a W a t e r Expected product size bp Primer set 2 E6(274) Forward and E6(388) Reverse If bands present for both E6 regions PCR products purified and sent for sequencing
20 Sequence file and Chromatograph Further analysis of chromatograph where N present in BioEdit and corrected if possible
21 Sequence Analysis Identified variants using pairwise alignment and MView tools Sequences compared to the reference genome (k ) and published variant sequences Phylogenetic analysis and clades defined Test associations of variants with disease outcomes
22 Nucleotide Position - Substitution Group No of VIN Cases No. of VSCC cases Reference T A G A C G T T E350G G 28 4 E135C... C E131G. G..... G 3 0 E131G.2. G E109C C G 2 1 As-178G G. 2 1 Af-2 C. T. G T.. 0 1
23 E109 C (A1) E350 G (A1) 32 cases E131G (A2) E131G.2 E135 C European Phenotype/Reference (A1) 53 Cases Af-2 (C) As-178G (A4 or European Asian)
24
25 SNP analysis All vulvar samples SNP genotyped using real time PCR and meltcurve analysis Primer/probes designed to amplify SNP rs Different alleles have different melting temperatures Melting peak profile determines genotype
26 Homozygous G/G Homozygous C/C Heterozygous G/C Water
27
28 G/G
29 G/G
30 Nil
31 G/G
32 G/C
33 G/G
34 HPV Negative Disease Allele Frequency n G C Controls % 7% VIN+VSCC 77 92% 8% VIN 33 91% 9% VSCC 37 93% 7% HPV Positive Disease Allele Frequency n G C Controls 84 93% 7% VIN+VSCC % 10% VIN % 9% VSCC 23 80% 20%
35
36
37 Is the C allele associated with increased risk of HPV positive VSCC?
38 G C HeLa transfected with GFP plasmid HeLa transfected with G Allele plasmid HeLa transfected with C Allele plasmid
39 Summary HPV important casual factor in VIN and VSCC Viral variants and Host SNPs may play a role in disease development and outcome Paucity of knowledge within vulvar disease First Scottish study regarding HPV genotype and variant prevalence Similar HPV prevalence compared to worldwide data Provides important information when considering prophylactic vaccination strategies CXCR1 SNP Interesting! Identify host and viral factors to enable patient stratification and enhance clinical management
40 Acknowledgements Dr Kate Cuschieri Professor Simon Herrington Professor Juergen Haas Professor Tim Aitman Dr Ramya Bhatia Professor Mark Arends Professor Sarah Howie Professor David Harrison Dr Katie Wakeham Dr Richard Weller Duanduan Cong Itziar Serrano Catherine Moore Daniel Guerendiain Norman Stenhouse
41 Functional Work Site Directed Mutagenesis Plasmid with CXCR1 gene with C allele and G allele produced HEK293 Cells Transfected with plasmids Chemotaxis assay
42 Negative Control HEK293 cells No Transfection Positive Control MM-6 cells HEK293- transfected with C allele plasmid HEK293- transfected with G allele plasmid
Prevalence of human papillomavirus and Chlamydia trachomatis infection in paired urine and cervical smear samples of Palestinian young women.
Prevalence of human papillomavirus and Chlamydia trachomatis infection in paired urine and cervical smear samples of Palestinian young women. Walid Salim Basha, PhD Faculty of Human Medicine An-Najah National
More informationPrevalence of HPV-16 genomic variant carrying a 63-bp duplicated sequence within the E1 gene in Slovenian women
Prevalence of HPV-16 genomic variant carrying a 63-bp duplicated sequence within the E1 gene in Slovenian women K E Y WORDS HPV-16, cervical cancer, E6-T350G variant A BSTRACT High-risk HPV, particularly
More informationHPV and Lower Genital Tract Disease. Simon Herrington University of Edinburgh, UK Royal Infirmary of Edinburgh, UK
HPV and Lower Genital Tract Disease Simon Herrington University of Edinburgh, UK Royal Infirmary of Edinburgh, UK Conflict of interest/funding X None Company: Product royalties Paid consultant Research
More informationOTKA azonosító: Típus: K Vezető kutató: Gergely Lajos
Human herpesvirus 6 (HHV-6), mostly variant B reactivation in renal transplant patients has been published previously, but the pathogenetic role of HHV-6 variant A has not been clarified. During the project
More informationInvestigation of the genetic differences between bovine herpesvirus type 1 variants and vaccine strains
Investigation of the genetic differences between bovine herpesvirus type 1 variants and vaccine strains Name: Claire Ostertag-Hill Mentor: Dr. Ling Jin Bovine herpesvirus Bovine herpesvirus-1 (BHV-1) Pathogen
More informationHuman Papillomavirus
Human Papillomavirus Dawn Palaszewski, MD Assistant Professor of Obstetrics and Gynecology University of February 18, 2018 9:40 am Dawn Palaszewski, MD Assistant Professor Department of Obstetrics and
More informationTowards the elimination of HPV
Towards the elimination of HPV Richard Hillman June 11th 2018 Potential conflicts of interest Potential Conflicts of Interest Declaration CSL research + travel + support for student MSD International Scientific
More informationSupplemental Materials and Methods Plasmids and viruses Quantitative Reverse Transcription PCR Generation of molecular standard for quantitative PCR
Supplemental Materials and Methods Plasmids and viruses To generate pseudotyped viruses, the previously described recombinant plasmids pnl4-3-δnef-gfp or pnl4-3-δ6-drgfp and a vector expressing HIV-1 X4
More informationHPV-Associated Disease and Prevention
HPV-Associated Disease and Prevention Odessa Regional Medical Center May 28, 2015 Erich M. Sturgis, MD, MPH Professor Department of Head & Neck Surgery Department of Epidemiology Christopher & Susan Damico
More informationTo test the possible source of the HBV infection outside the study family, we searched the Genbank
Supplementary Discussion The source of hepatitis B virus infection To test the possible source of the HBV infection outside the study family, we searched the Genbank and HBV Database (http://hbvdb.ibcp.fr),
More informationINTRODUCTION HUMAN PAPILLOMAVIRUS
INTRODUCTION HUMAN PAPILLOMAVIRUS Professor Anna-Lise Williamson Institute of Infectious Disease and Molecular Medicine, University of Cape Town National Health Laboratory Service, Groote Schuur Hospital
More informationDrug Metabolism Disposition
Drug Metabolism Disposition The CYP2C19 intron 2 branch point SNP is the ancestral polymorphism contributing to the poor metabolizer phenotype in livers with CYP2C19*35 and CYP2C19*2 alleles Amarjit S.
More informationHPV FREE IDAHO. Fundamentals of HPV Bill Atkinson, MD MPH
HPV FREE IDAHO Fundamentals of HPV Bill Atkinson, MD MPH You are the Key to HPV Cancer Prevention William Atkinson, MD, MPH Associate Director for Immunization Education Immunization Action Coalition February
More informationComparative Phylogenetic Analysis of E6 and E7 Proteins of Different 42 Strains of HPV Sangeeta Daf*, Lingaraja Jena, Satish Kumar
ORIGINAL ARTICLE Comparative Phylogenetic Analysis of E6 and E7 Proteins of Different 42 Strains of HPV Sangeeta Daf*, Lingaraja Jena, Satish Kumar Abstract Simultaneous phylogenetic analysis of E6 and
More informationHost Genomics of HIV-1
4 th International Workshop on HIV & Aging Host Genomics of HIV-1 Paul McLaren École Polytechnique Fédérale de Lausanne - EPFL Lausanne, Switzerland paul.mclaren@epfl.ch Complex trait genetics Phenotypic
More informationAlexandra Lydia Hernandez. A dissertation submitted in partial satisfaction of the. requirements for the degree of. Doctor of Philosophy.
Human papillomavirus infection in HIV-seropositive men who have sex with men in both the United States and India: Prevalence, incidence, and risk factors for infection by Alexandra Lydia Hernandez A dissertation
More informationBattle against Human Papilloma Virus (HPV): Expanded Vaccine Recommendations
Battle against Human Papilloma Virus (HPV): Expanded Vaccine Recommendations Sean W. Clark, Pharm.D. PGY-2 Ambulatory Care Resident Duquesne University and The Center for Pharmacy Care I have no relevant
More informationThe impact of the HPV vaccine in Scotland.
The impact of the HPV vaccine in Scotland Kevin.pollock@nhs.net Cervical cancer by deprivation Scotland 18 Cancer of the cervix uteri (ICD-10 C53) Age-standardised incidence and mortality rates by SIMD
More informationAppropriate Use of Cytology and HPV Testing in the New Cervical Cancer Screening Guidelines
Appropriate Use of Cytology and HPV Testing in the New Cervical Cancer Screening Guidelines Tim Kremer, MD Ralph Anderson, MD 1 Objectives Describe the natural history of HPV particularly as it relates
More informationSupplementary Figure 1. Quantile-quantile (Q-Q) plots. (Panel A) Q-Q plot graphical
Supplementary Figure 1. Quantile-quantile (Q-Q) plots. (Panel A) Q-Q plot graphical representation using all SNPs (n= 13,515,798) including the region on chromosome 1 including SORT1 which was previously
More informationOPPORTUNISTIC HPV VACCINATION: AN EXPANDED VISION
OPPORTUNISTIC HPV VACCINATION: AN EXPANDED VISION SUMMARY POSITION Human papillomavirus (HPV) infection is preventable but not adequately prevented. At present, Canada has a robust school vaccination program
More informationProphylactic HPV Vaccines. Margaret Stanley Department of Pathology Cambridge
Prophylactic HPV Vaccines Margaret Stanley Department of Pathology Cambridge 8kb double stranded DNA viruses, absolutely host and tissue specific, Can t grow virus in tissue culture Classified by genotype
More informationPathology of the Cervix
Pathology of the Cervix Thomas C. Wright Pathology of the Cervix Topics to Consider Burden of cervical cancer 1 Invasive Cervical Cancer Cervical cancer in world Second cause of cancer death in women Leading
More informationObjectives. Disclosures. HPV: Quick Facts
Objectives Identify the prevalence of HPV and HPVrelated malignancies across Texas Understand the impact of HPV vaccination on HPV-malignancies Describe cancer survivors risk for HPVrelated malignancies
More informationThe Value of the Residual "HPV Load" after Surgical Treatment of Cervical Intraepithelial Neoplasia
ISSN: 2578-4838 The Value of the Residual "HPV Load" after Surgical Treatment of Cervical Intraepithelial Neoplasia Cherenkov VG*, Alexandrov AS and Sycheva DN Novgorod State University, Yaroslav the Wise,
More informationCorrelation of HPV Types in Archived ASCUS Samples
Correlation of HPV Types in Archived ASCUS Samples Sharon Wah-Suet NG 1, Stephen Tsui-Hoi Lo 2, Daniel Chuen-Chu TAM 1 1 Genepath Technology Limited 2 Molecular Pathology Laboratory, Department of Pathology,
More informationHUMAN PAPILLOMAVIRUS VACCINES AND CERVICAL CANCER
HUMAN PAPILLOMAVIRUS VACCINES AND CERVICAL CANCER Virology The Human Papillomavirus (HPV) is a relatively small virus, belonging to the family Papillomaviridae, containing circular double-stranded DNA
More informationUpdate of the role of Human Papillomavirus in Head and Neck Cancer
Update of the role of Human Papillomavirus in Head and Neck Cancer 2013 International & 12 th National Head and Neck Tumour Conference Shanghai, 11 13 Oct 2013 Prof. Paul KS Chan Department of Microbiology
More informationCorrelation between Vitamin D Receptor SNP ApaI and liver fibrosis in HCV patients from Thrace
Correlation between Vitamin D Receptor SNP ApaI and liver fibrosis in HCV patients from Thrace A. Beka 1, T. Mylopoulou 2, V. Papadopoulos 2, I. Karakasiliotis 2, P. Mavromara 1, K. Mimidis 2, S. Veletza
More informationDetection and significance of PD-1.3 SNP (rs ) and IL28B SNP (rs ) in patients with current or past hepatitis B virus (HBV) infection
Detection and significance of PD-1.3 SNP (rs11568821) and IL28B SNP (rs12979860) in patients with current or past hepatitis B virus (HBV) infection Asterios Saitis 1, Nikolaos K. Gatselis 1, Kalliopi Azariadi
More informationSupplementary Appendix
Supplementary Appendix This appendix has been provided by the authors to give readers additional information about their work. Supplement to: Garland SM, Hernandez-Avila M, Wheeler CM, et al. Quadrivalent
More informationPre-Assessment Review: Microbiology, Part 2: Virology. Dr. David Hillyard
Pre-Assessment Review: Microbiology, Part 2: Virology Dr. David Hillyard 1. Mutations or deletions within which gene are associated with the hyper-toxin producing strain of Clostridium difficile? a. tcda
More informationWorkshop Report: HPV and Cancer: Implications for Clinical Trials
Workshop Report: HPV and Cancer: Implications for Clinical Trials On Thursday 15 th November 2012, the National Cancer Research Institute (NCRI) Clinical Studies Groups (CSGs), partly supported by the
More informationCERVIX. MLS Basic histological diagnosis MLS HIST 422 Semester 8- batch 7 L12 : Dr. Ali Eltayb.
CERVIX MLS Basic histological diagnosis MLS HIST 422 Semester 8- batch 7 L12 : Dr. Ali Eltayb. CERVIX Most cervical lesions are: Most are Cervicitis. cancers ( common in women worldwide). CERVICITIS Extremely
More informationSmoking, human papillomavirus infection, and p53 mutation as risk factors in oropharyngeal cancer: a case-control study
RESEARCH FUND FOR THE CONTROL OF INFECTIOUS DISEASES Smoking, human papillomavirus infection, and p53 as risk factors in oropharyngeal cancer: a case-control study PKS Chan *, JSY Chor, AC Vlantis, TL
More information9/11/2018. HPV Yoga. Human Papillomavirus. Human Papillomavirus (HPV) Disease. Most common sexually transmitted infection in the U.S.
Centers for Disease Control and Prevention National Center for Immunization and Respiratory Diseases Human Papillomavirus September 2018 Chapter 11 Photographs and images included in this presentation
More informationIdentification of Mutation(s) in. Associated with Neutralization Resistance. Miah Blomquist
Identification of Mutation(s) in the HIV 1 gp41 Subunit Associated with Neutralization Resistance Miah Blomquist What is HIV 1? HIV-1 is an epidemic that affects over 34 million people worldwide. HIV-1
More informationScreening for Cervical Cancer. Grand Rounds 1/16/13 Meggan Linck
Screening for Cervical Cancer Grand Rounds 1/16/13 Meggan Linck Cervical Cancer Worldwide 2 nd most common and 5 th deadliest U.S. 8 th most common 80% occur in developing world Median age at diagnosis
More informationThe Role of Epidemiologic Studies in Establishing HPV as a Cause of Cancer. April 2, 2008 Margaret M. Madeleine, PhD. Outline
The Role of Epidemiologic Studies in Establishing HPV as a Cause of Cancer April 2, 2008 Margaret M. Madeleine, PhD Outline HPV in Cervical Cancer Basics: Incidence, screening, and the virus Causal criteria
More informationCHAPTER IV RESULTS Microcephaly General description
47 CHAPTER IV RESULTS 4.1. Microcephaly 4.1.1. General description This study found that from a previous study of 527 individuals with MR, 48 (23 female and 25 male) unrelated individuals were identified
More informationFocus. International #52. HPV infection in High-risk HPV and cervical cancer. HPV: Clinical aspects. Natural history of HPV infection
HPV infection in 2014 Papillomaviruses (HPV) are non-cultivable viruses with circular DNA. They can establish productive infections in the skin (warts) and in mucous membranes (genitals, larynx, etc.).
More informationHPV and Oral Cancer. Oral Cancer
HPV and Oral Cancer How do we prevent oral cancer? Dr. Peter Angeletti Oral Cancer 3% of all cancers in the US are in oral compartment 8 th most common in men 30,000 new cases each year 8,000 deaths each
More informationThe Global Burden of HPV Related Cancers and Their Prevention
The Global Burden of HPV Related Cancers and Their Prevention What Every Doctor Should Know! Dr. Adrian Lear, MD Conflict of Interest Disclosure I have no financial relations with any commercial entity
More informationHPV Epidemiology and Natural History
HPV Epidemiology and Natural History Rachel Winer, PhD, MPH Associate Professor Department of Epidemiology University of Washington School of Public Health rlw@uw.edu Human Papillomavirus (HPV) DNA virus
More informationTHE ROLE OF microrna POLYMORPHISMS IN GASTRIC CANCER PATHOGENESIS
UNIVERSITY OF MEDICINE AND PHARMACY CRAIOVA DOCTORAL SCHOOL THE ROLE OF microrna POLYMORPHISMS IN GASTRIC CANCER PATHOGENESIS PhD THESIS ABSTRACT SCIENTIFIC ADVISOR: PROF. UNIV. DR. ION ROGOVEANU PhD student:
More informationRisk factors for precancerous lesions of the cervix in a population of Georgian women
Training Course in Sexual and Reproductive Health Research Geneva, 2 March 2009 Risk factors for precancerous lesions of the cervix in a population of Georgian women Maia Chiokadze, M.D. Medical Diagnostic
More informationRole of Paired Box9 (PAX9) (rs ) and Muscle Segment Homeobox1 (MSX1) (581C>T) Gene Polymorphisms in Tooth Agenesis
EC Dental Science Special Issue - 2017 Role of Paired Box9 (PAX9) (rs2073245) and Muscle Segment Homeobox1 (MSX1) (581C>T) Gene Polymorphisms in Tooth Agenesis Research Article Dr. Sonam Sethi 1, Dr. Anmol
More informationHPV AND CERVICAL CANCER
HPV AND CERVICAL CANCER DR SANDJONG TIECHOU ISAAC DELON Postgraduate Training in Reproductive Health Research Faculty of Medicine, University of Yaoundé 2007 INTRODUCTION CERVICAL CANCER IS THE SECOND
More informationfor Microbiology Novos programas de Controlo de Qualidade Externo: desenvolvimento e perspectivas 15 and 16 October 2008 Biognóstica - Portugal
for Microbiology Novos programas de Controlo de Qualidade Externo: desenvolvimento e perspectivas Overview Development of new schemes Molecular detection of mycobacteria Introduced as a new scheme in 2007
More informationThe HPV Vaccination Programme Early intervention in cancer prevention Northern Ireland
The HPV Vaccination Programme Early intervention in cancer prevention Northern Ireland Immunisations Very cost effective intervention Give vaccine before exposure to disease UK has life course approach
More informationBottoms UP HIV and Anal Cancer from Screening to Prevention
Bottoms UP HIV and Anal Cancer from Screening to Prevention Paul MacPherson PhD, MD, FRCPC Associate Professor Division of Infectious Diseases University of Ottawa The New Reality Normal or near-normal
More informationSupplementary information
Supplementary information Hepatitis B virus genotype, mutations, human leukocyte antigen polymorphisms and their interactions in hepatocellular carcinoma: a multi-centre case-control study Juan Wen, Ci
More informationGenetic variation at the IFITM3 and Influenza A(H1N1)pdm09 infection severity in the Portuguese population
Genetic variation at the IFITM3 and Influenza infection severity in the Portuguese population Vânia Gaio 1, Baltazar Nunes 1, Pedro Pechirra 2, Patrícia Conde 2, Raquel Guiomar 2, Carlos Matias Dias 1,
More informationMultiple sequence alignment
Multiple sequence alignment Bas. Dutilh Systems Biology: Bioinformatic Data Analysis Utrecht University, February 18 th 2016 Protein alignments We have seen how to create a pairwise alignment of two sequences
More informationToll-like Receptors (TLRs): Biology, Pathology and Therapeutics
Toll-like Receptors (TLRs): Biology, Pathology and Therapeutics Dr Sarah Sasson SydPATH Registrar 23 rd June 2014 TLRs: Introduction Discovered in 1990s Recognise conserved structures in pathogens Rely
More informationGlobal HPV Disease Burden : Rationale for Vaccine
Global HPV Disease Burden : Rationale for Vaccine Muhammet Nabi Kanibir, MD Regional Medical Director Medical Affairs, MSD-Vaccines Muscat, September 2011 HPV Disease Burden Global Regional What do we
More informationThe Biology of HPV Infection and Cervical Cancer
The Biology of HPV Infection and Cervical Cancer Kaitlin Sundling, M.D., Ph.D. Clinical Instructor Faculty Director, Cytotechnology Program Wisconsin State Laboratory of Hygiene and University of Wisconsin
More information2 HPV E1,E2,E4,E5,E6,E7 L1,L2 E6,E7 HPV HPV. Ciuffo Shope Jablonska HPV Zur Hausen HPV HPV16. HPV-genome.
58 2 pp.155-164 2008 2. HPV 20 HPV HPV HPV HPV HPV L1 HPV-DNA 16/18 2 HPV16 /18 6/11 4 2 100 1 Ciuffo 1907 20 Shope 1933 Raus 1934 20 70 Jablonska HPV Orth HPV5 8 1983 zur Hausen HPV16 1 HPV-genome 920-8641
More informationCERVICAL CANCER FACTSHEET. What is cervical cancer?
CERVICAL CANCER FACTSHEET What is cervical cancer? ENGAGe is releasing a series of factsheets to raise awareness of gynaecological cancers and to support its network to work at a grassroots level. Take-up
More informationFocus. A case. I have no conflicts of interest. HPV Vaccination: Science and Practice. Collaborative effort with Karen Smith-McCune, MD, PhD 2/19/2010
HPV Vaccination: Science and Practice George F. Sawaya, MD Professor Department of Obstetrics, Gynecology and Reproductive Sciences Department of Epidemiology and Biostatistics Director, Colposcopy Clinic,
More informationDETECTION OF LOW FREQUENCY CXCR4-USING HIV-1 WITH ULTRA-DEEP PYROSEQUENCING. John Archer. Faculty of Life Sciences University of Manchester
DETECTION OF LOW FREQUENCY CXCR4-USING HIV-1 WITH ULTRA-DEEP PYROSEQUENCING John Archer Faculty of Life Sciences University of Manchester HIV Dynamics and Evolution, 2008, Santa Fe, New Mexico. Overview
More informationThe promise of HPV vaccines for Cervical (and other genital cancer) prevention
The promise of HPV vaccines for Cervical (and other genital cancer) prevention CONTROVERSIES IN OBSTETRICS GYNECOLOGY & INFERTILITY Barcelona March 27 F. Xavier Bosch Catalan Institute of Oncology CURRENT
More informationMaking Sense of Cervical Cancer Screening
Making Sense of Cervical Cancer Screening New Guidelines published November 2012 Tammie Koehler DO, FACOG The incidence of cervical cancer in the US has decreased more than 50% in the past 30 years because
More informationWoo Dae Kang, Ho Sun Choi, Seok Mo Kim
Is vaccination with quadrivalent HPV vaccine after Loop Electrosurgical Excision Procedure effective in preventing recurrence in patients with High-grade Cervical Intraepithelial Neoplasia (CIN2-3)? Chonnam
More informationRelationship between human papillomavirus infection and cervical cancer progression
21 Review Relationship between human papillomavirus and cervical cancer progression Meichang Ai Infection Management Section, The People's Hospital of Lincang, Lincang, China Keywords Human papillomavirus;
More informationANALYSIS OF MYCOPLASMA GENITALIUM STRAINS ISOLATED FROM PREGNANT WOMEN AT AN ACADEMIC HOSPITAL IN PRETORIA, SOUTH AFRICA
ANALYSIS OF MYCOPLASMA GENITALIUM STRAINS ISOLATED FROM PREGNANT WOMEN AT AN ACADEMIC HOSPITAL IN PRETORIA, SOUTH AFRICA Mafunise M 1, Le Roux MC 1, de Villiers BE 1, Ditsele RMM 1,2 1 Department of Microbiological
More informationPRODUCT INFORMATION GARDASIL 9. [Human Papillomavirus 9-valent (Types 6, 11, 16, 18, 31, 33, 45, 52, 58) vaccine, Recombinant]
PRODUCT INFORMATION GARDASIL 9 [Human Papillomavirus 9-valent (Types 6, 11, 16, 18, 31, 33, 45, 52, 58) vaccine, Recombinant] DESCRIPTION GARDASIL 9 *, Human Papillomavirus 9-valent Vaccine, Recombinant,
More informationObjectives. Background. Background. Background. Background 9/26/16. Update on Cervical and HPV Screening Guidelines: To pap or not to pap?
Update on Cervical and HPV Screening Guidelines: To pap or not to pap? Marina Delazari Miller MD Clinical Assistant Professor Department of Obstetrics & Gynecology University of Iowa Hospitals and Clinics
More informationNo HPV High Risk Screening with Genotyping. CPT Code: If Result is NOT DETECTED (x3) If Results is DETECTED (Genotype reported)
CPAL Central Pennsylvania Alliance Laboratory Technical Bulletin No. 117 August 6, 2013 HPV High Risk Screening with Genotyping Contact: Dr. Jeffrey Wisotzkey, 717-851-1422 Director, Molecular Pathology
More informationProf. Dr. Kutikuppala Surya Rao MD.,PhD.,MNAMS.,FHM(CMC Vellore) DFM.,FCGP.,FRCP(London)
A Study of HPV genotypes in HIV-Positive Women for early detection of risk for cervical cancers in Andhrapradesh, India. Prof. Dr. Kutikuppala Surya Rao MD.,PhD.,MNAMS.,FHM(CMC Vellore) DFM.,FCGP.,FRCP(London)
More informationHPV and PREGNANCY Arsenio Spinillo,, MD
HPV and PREGNANCY Arsenio Spinillo,, MD University of Pavia, Department of Obstetrics and Gynecology, IRCCS Fondazione Policlinico San Matteo di Pavia Human Papilloma Virus HPV is a member of the Papovaviridae
More informationHepatitis B Virus Genemer
Product Manual Hepatitis B Virus Genemer Primer Pair for amplification of HBV Viral Specific Fragment Catalog No.: 60-2007-10 Store at 20 o C For research use only. Not for use in diagnostic procedures
More informationCHR POS REF OBS ALLELE BUILD CLINICAL_SIGNIFICANCE
CHR POS REF OBS ALLELE BUILD CLINICAL_SIGNIFICANCE is_clinical dbsnp MITO GENE chr1 13273 G C heterozygous - - -. - DDX11L1 chr1 949654 A G Homozygous 52 - - rs8997 - ISG15 chr1 1021346 A G heterozygous
More informationSALSA MLPA probemix P241-D2 MODY mix 1 Lot D As compared to version D1 (lot D1-0911), one reference probe has been replaced.
mix P241-D2 MODY mix 1 Lot D2-0413. As compared to version D1 (lot D1-0911), one reference has been replaced. Maturity-Onset Diabetes of the Young (MODY) is a distinct form of non insulin-dependent diabetes
More informationUNIVERSITY OF MEDICINE AND PHARMACY OF CRAIOVA DOCTORAL SCHOOL DOCTORAL THESIS
UNIVERSITY OF MEDICINE AND PHARMACY OF CRAIOVA DOCTORAL SCHOOL DOCTORAL THESIS PHARMACOGENETICS AND THE APPLICATION OF SINGLE NUCLEOTIDE POLYMORPHISMS IN RESPONSE TO PEGYLATED INTERFERON AND RIBAVIRIN
More informationHPV Infection in Fe-male Patients: Methods of Detection. Topics. West Coast Pathology Laboratories WEST COAST PATHOLOGY LABORATORIES
WEST COAST PATHOLOGY LABORATORIES HPV Infection in Fe-male Patients: Methods of Detection John Compagno, MD, Medical Director Topics Pap cytology still an excellent test made better Value of HPV Genotyping
More informationFONS Nové sekvenační technologie vklinickédiagnostice?
FONS 2010 Nové sekvenační technologie vklinickédiagnostice? Sekvenování amplikonů Sequence capture Celogenomové sekvenování FONS 2010 Sekvenování amplikonů Amplicon sequencing - amplicon sequencing enables
More informationPrevalence Of Precancerous Lesions Of The Uterine Cervix
Prevalence Of Precancerous Lesions Of The Uterine Cervix 1 / 6 2 / 6 3 / 6 Prevalence Of Precancerous Lesions Of INTRODUCTION. Gastric intestinal metaplasia is an intermediate precancerous gastric lesion
More informationViral genome sequencing: applications to clinical management and public health. Professor Judy Breuer
Viral genome sequencing: applications to clinical management and public health Professor Judy Breuer Why do whole viral genome sequencing Genome sequencing allows detection of multigenic resistance in
More informationDone by khozama jehad. Neoplasia of the cervix
Done by khozama jehad Neoplasia of the cervix An overview of cervical neoplasia very import. Most tumors of the cervix are of epithelial origin and are caused by oncogenic strains of human papillomavirus
More informationHPV Vaccination Rates
HPV Vaccination Rates Jennifer E. Dietrich MD, MSc Fellowship Director Pediatric and Adolescent Gynecology, Division of Pediatric and Adolescent Gynecology Department of Obstetrics and Gynecology Department
More informationReporting TP53 gene analysis results in CLL
Reporting TP53 gene analysis results in CLL Mutations in TP53 - From discovery to clinical practice in CLL Discovery Validation Clinical practice Variant diversity *Leroy at al, Cancer Research Review
More informationClinical Relevance of HPV Genotyping. A New Dimension In Human Papillomavirus Testing. w w w. a u t o g e n o m i c s. c o m
Clinical Relevance of HPV Genotyping A New Dimension In Human Papillomavirus Testing Human Papillomavirus: Incidence HPV prevalence was 26.8% for women in US aged 14 59 yrs 1 20 million Americans are currently
More informationMandatory HPV Vaccination: An Ethical Analysis
Volume 2 Number 1 (Spring/Summer 2016) www.jheaonline.org. ARTICLE Mandatory HPV Vaccination: An Ethical Analysis This article appeared originally in The Internet Journal of Catholic Bioethics (Winter
More informationSupplemental Information For: The genetics of splicing in neuroblastoma
Supplemental Information For: The genetics of splicing in neuroblastoma Justin Chen, Christopher S. Hackett, Shile Zhang, Young K. Song, Robert J.A. Bell, Annette M. Molinaro, David A. Quigley, Allan Balmain,
More information5. Over the last ten years, the proportion of HIV-infected persons who are women has: a. Increased b. Decreased c. Remained about the same 1
Epidemiology 227 April 24, 2009 MID-TERM EXAMINATION Select the best answer for the multiple choice questions. There are 60 questions and 9 pages on the examination. Each question will count one point.
More informationComputational Systems Biology: Biology X
Bud Mishra Room 1002, 715 Broadway, Courant Institute, NYU, New York, USA L#4:(October-0-4-2010) Cancer and Signals 1 2 1 2 Evidence in Favor Somatic mutations, Aneuploidy, Copy-number changes and LOH
More informationSCCPS Scientific Committee Position Paper on HPV Vaccination
SCCPS Scientific Committee Position Paper on HPV Vaccination Adapted from Joint Statement (March 2011) of the: Obstetrical & Gynaecological Society of Singapore (OGSS) Society for Colposcopy and Cervical
More informationHuman papillomavirus and vaccination for cervical cancer
Human papillomavirus and vaccination for cervical cancer Dorothy Machalek Department of Microbiology and Infectious Diseases Royal Women s Hospital, Melbourne, Australia VIRUSES AND CANCER Responsible
More informationO RIGINAL P APER. Extending Quadrivalent Human Papilloma Virus (HPV) Vaccination To Males What Is The Current Evidence?
O RIGINAL P APER Extending Quadrivalent Human Papilloma Virus (HPV) Vaccination To Males What Is The Current Evidence? T H E S I N G A P O R E F A M I L Y P H Y S I C I A N V O L 4 1(3) J U L - S E P 2
More informationa) SSR with core motif > 2 and repeats number >3. b) MNR with repeats number>5.
1 2 APPENDIX Legends to figures 3 4 5 Figure A1: Distribution of perfect SSR along chromosome 1 of V. cholerae (El-Tor N191). a) SSR with core motif > 2 and repeats number >3. b) MNR with repeats number>5.
More informationChapters 21-26: Selected Viral Pathogens
Chapters 21-26: Selected Viral Pathogens 1. DNA Viral Pathogens 2. RNA Viral Pathogens 1. DNA Viral Pathogens Smallpox (pp. 623-4) Caused by variola virus (dsdna, enveloped): portal of entry is the respiratory
More informationAhmedin Jemal, DVM, PhD American Cancer Society
The 2 nd Edition of The Cancer Atlas Cancer Interventions and Potential for Impact Ahmedin Jemal, DVM, PhD American Cancer Society Session code: CTS.4.230 www.worldcancercongress.org Interventions Across
More informationOvarian function and pregnancy after Hematopoietic Stem Cell Transplant
Ovarian function and pregnancy after Hematopoietic Stem Cell Transplant Factors that influence post transplantation fertility and ovarian function in women Total body irradiation (TBI) Drugs prescribed
More informationClinically Microscopically Pathogenesis: autoimmune not lifetime
Vulvar Diseases: Can be divided to non-neoplastic and neoplastic diseases. The neoplastic diseases are much less common. Of those, squamous cell carcinoma is the most common. most common in postmenopausal
More informationHIV in Obstetrics and Gynecology
FAST FACTS HIV in Obstetrics and Gynecology Indispensable Guides to Clinical by J Richard Smith, Naomi Low-Beer and Bruce A Barron Practice HIV infection 7 Managing infected women 13 Preconceptual care
More informationHPV is the most common sexually transmitted infection in the world.
Hi. I m Kristina Dahlstrom, an instructor in the Department of Head and Neck Surgery at The University of Texas MD Anderson Cancer Center. My lecture today will be on the epidemiology of oropharyngeal
More informationHPV Infection and associated disease among HIV positive individuals. Admire Chikandiwa. Wits RHI
HPV Infection and associated disease among HIV positive individuals Admire Chikandiwa Wits RHI Outline of presentation Introduction Burden of HPV associated diseases The role of HIV and its interaction
More informationHIV-infected men and women. Joel Palefsky, M.D. University of California, San Francisco
Anal cytology and anal cancer in HIV-infected men and women. Joel Palefsky, M.D. University of California, San Francisco April 10, 2010 Disclosures Merck and Co: Research grant support, advisory boards
More informationCELL BIOLOGY - CLUTCH CH CANCER.
!! www.clutchprep.com CONCEPT: OVERVIEW OF CANCER Cancer is a disease which is primarily caused from misregulated cell division, which form There are two types of tumors - Benign tumors remain confined
More information