Panel of Rat cell lines
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1 Panel of Rat cell s Part of the CLS cell bank CLS Cell Lines Service Table 1: Rat cell s: Origin and General Characteristics Name of cell Cell type Organism, Ethnicity Age / Gender Tissue / Disease Morphology Growth properties AH AH-130 FN 2 AR42J 3 AS-30D 4 AT-1 5 Rat Pancreas Pancreatic tumor cell Rat Prostate Adenocarcinoma cell Wistar Copenhagen rats BRL-3A 6 Rat Liver cell Buffalo 16 month / Female Ascites hepatoma Ascites hepatoma Pancreas tumor, exocrine Hepatoma Rat dorsal prostatic adenocarcinoma Round suspension cells, triangular Round cells in suspension, epithelial in the state Round cells, loosely, floating Suspension / Adherent Suspension, few Cluster, appear as hollow spheroid colonies. can pile up and attach loosely Suspension / Liver C6 7 Rat Brain Glioma cells Brain (glioma) Fibroblast CC531 8 CLS-ACI-1 9 DSL6A-C1 10 DSL-6B-C2 10 Rat Colon Adenocarcinoma cell Rat Breast Adenocarcinoma cell Rat Pancreas Carcinoma, ductal cell Rat Pancreas Carcinoma, ductal cell WAG-Rij-rats ACI Lewis Lewis FRTL 11 Rat Thyroidea cell Fischer 3 months / Female Colon carcinoma Breast Epitheloid and lymphoblastoidlike Suspension / Male Pancreas carcinoma Male Pancreas carcinoma Weeks Thyroid, normal CLS order no info@clsgmbh.de website: Page 1 of 6
2 Name of cell Cell type Organism, Ethnicity Age / Gender Tissue / Disease Morphology Growth properties FRTL-5 12 Rat Thyroidea cell Fischer 344 HB-CLS-3 LC MH-3924A 14 MH-TC Novikoff Hepatoma 16 Rat Urinary bladder Carcinoma cell Rat Testis Leydig cell tumor cell ACI Fischer ACI Buffalo NRK-49F 17 Rat Kidney cell Osborne-Mendel (OM) O Rat Ovary Adenocarcinoma cell OS-1 CLS PC RBL-1 20 RTE-2 21 RY Yoshida SArcoma 22 ZH Zajdela Hepatoma 23 Rat Bone Osteosarcoma cell Rat Adrenal gland Pheochromocytoma cell Rat Hematopoiesis basophil leukemia cell Rat Tongue keratinocyte cell Rat Fibroblast sarcoma cell Rat Liver hepatoma cell Thyroid, normal Adherent, colonies 1 year / Male Bladder, urinary, Squamous cell carcinoma Adult / Male 16 months / Male Female Male Wistar Leydig cell tumor; testis Adherent, colonies Adherent, colonies Liver, hepatoma Epitheloid 10 months Liver, hepatoma Epitheloid Liver, hepatoma Epitheloid Suspension, few cells Kidney, normal Fibroblast-like Ovary adenocarcinoma Bone osteosarcoma Adrenal gland, Pheochromocytoma Blood (chemically induced leukemia) Lymphoblast, basophil Polygonal Tongue, keratinocyte Small clusters in suspension, poorly ; patches on collagen Round cells Suspension / Ascites Sarcoma Suspension / 11 months Liver, hepatoma CLS order no info@clsgmbh.de website: Page 2 of 6
3 Table 2: Rat cell s: Special Features Name of cell AH Cell type Karyotype Virus Resistance Virus Susceptibility Secretion of products Ref ID in Cellosaurus 24 AH-130 FN 2 AR42J 3 AS-30D 4 AT-1 5 Rat Pancreas Pancreatic tumor cell Rat Prostate Adenocarcinoma cell Hypodiploid rat karyotype with 12% tetraploidy; 38 (35-41). Very sensitive to human adenoviruses Amylase and other exocrine enzymes Insulin; glucocorticoid Amylase and other exocrine enzymes Insulin; glucocorticoid BRL-3A 6 Rat Liver cell Multiplication stimulating activity (MSA). C6 7 Rat Brain Glioma cells Poliovirus 3 vesicular stomatitis (Indiana); vaccinia; herpes simplex neg Glucocorticoid CC531 8 Rat Colon Adenocarcinoma cell CLS-ACI-1 9 Rat Breast Adenocarcinoma cell Near triploid. 88.4% showing chromosomes, 5% chromosomes, 6.6% near tetraploid or higher ploidy level. Overexpression of Mycn gene. CLS order no. RRID:CVLL_ RRID:CVLL_ RRID:CVLL_ RRID:CVLL_ RRID:CVLL_ RRID:CVLL_ RRID:CVLL_ RRID:CVLL_ RRID:CVLL_ info@clsgmbh.de website: Page 3 of 6
4 Name of cell DSL-6A-C1 10 Rat Pancreas Carcinoma, ductal cell DSL-6B-C2 10 Rat Pancreas Carcinoma, ductal cell Cell type Karyotype Virus Resistance Virus Susceptibility Secretion of products Ref ID in Cellosaurus 24 Expression: ductal marker cystic fibrosis transmembrane regulator (CFTR), Amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture Lost structural and immunohistochemical acinar cell markers Mucin Amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture Lost structural and immunohistochemical acinar cell markers FRTL 11 Rat Thyroidea cell Diploid Thyroglobulin accumulate iodide intracellularly FRTL-5 12 Rat Thyroidea cell Thyroglobulin Thyroid stimulating hormone (TSH) IFN-γ induced expression of HLA-DR HB-CLS-3 LC Rat Urinary bladder Carcinoma cell Rat Testis Leydig cell tumor cell MH-3924A 14 MH-TC Novikoff Hepatoma 16 Steroid hormone; estrogen (estradiol and others); androgen (testosterone and others) Reverse Transcriptase: pos CLS order no. RRID:CVLL_ RRID:CVLL_W RRID:CVLL_ RRID:CVLL_ RRID:CVLL_ RRID:CVLL_ RRID:CVLL_ RRID:CVLL_ RRID:CVLL_1D info@clsgmbh.de website: Page 4 of 6
5 Name of cell Cell type Karyotype Virus Resistance Virus Susceptibility Secretion of products Ref ID in Cellosaurus 24 NRK-49F 17 Rat Kidney cell Epidermal growth factor (EGF); multiplication stimulating activity (MSA) O Rat Ovary Adenocarcinoma cell OS-1 CLS PC RBL-1 20 RTE-2 21 RY Yoshida Sarcoma 22 ZH Zajdela Hepatoma 23 Rat Bone Osteosarcoma cell Rat Adrenal gland Pheochromocytoma cell Rat Hematopoiesis basophil leukemia cell Rat Tongue keratinocyte cell Rat Fibroblast sarcoma cell Rat Liver hepatoma cell 40 chromosomes; 38 autosomes plus XY Information on cell culture conditions, authentication data and others can be found on the website: CLS order no. RRID:CVLL_ RRID:CVLL_ RRID:CVLL_ Catecholamines; dopamine; RRID:CVLL_S norepinephrine Nerve growth factor (NGF) Histamine Fc of IgE RRID:CVLL_ RRID:CVLL_ RRID:CVLL_1D RRID:CVLL_1D website: Page 5 of 6
6 References:. 1. Hoshino M. Submicroscopic characteristics of four strains of Yoshida ascites hepatoma of rats: a comparative study. Cancer Res. 23:209-16, Mutoh S et al. The isolation and characterization of glycopeptides and mucopolysaccharides from plasma membranes of an ascites hepatoma, AH 130 FN. J Biochem (Tokyo) 80: , Longnecker DS et al. Effect of age on nodule induction by azaserine and DNA synthesis in rat pancreas. J Natl Cancer Inst 58: , Smith DF and Walborg EF Jr. Isolation and chemical characterization of cell surface sialoglycopeptide fractions during progression of rat ascites hepatoma AS-30D. Cancer Res 32: 543-9, Isaacs JT et al. Establishment and characterization of seven Dunning rat prostatic cancer cell s and their use in developing methods for predicting metastatic abilities of prostatic cancers. Prostate 9: , Coon HG and Weiss MC. A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc Natl Acad Sci USA 62: 852-9, Benda P et al. Differentiated rat glial cell strain in tissue culture. Science 161: 370, Thomas C, Nijenhuis AM, Timens W, Kuppen PJ, Daemen T, Scherphof GL. Liver metastasis model of colon cancer in the rat: immunohistochemical characterization. Invasion- Metastasis 13(2):102-12, Meles S, Adega F, Castro J and Chaves R. Cytogenetic Assessment of the Rat Cell Line CLS-ACI-1: An in vitro Cell Model for Mycn Overexpression. Cytogenet Genome Res 146: , Pettengill 0.S., R. A. Faris,t R. H. Bell, Jr., E. T. Kuhlmann and D. S. Longnecker. Derivation of Ductlike Cell Lines from a Transplantable Acinar Cell Carcinoma of the Rat Pancreas. Am. J. Pathol. 143: , Ambesi-Impiombato FS et al. Culture of hormone-dependent functional epithelial cells from rat thyroids. Proc. Natl. Acad. Sci. USA 77: , Ambesi-Impiombato FS. Living, fast-growing thyroid cell strain, FRTL-5. U.S. Pat. 4,608,341, Jacobs BB et al. Transplantable Leydig cell tumors in Fischer rats: hormone responsibility and hormone production. J Natl Cancer Inst 41: , Chang L.O. et al. Comparative incorporation of tritiated thymidine and cytidine into the mitochondrial and nuclear DNA and RNA of two transplantable hepatomas (3924A and h-35tc2) and host livers. Cancer Res 28: , Brix G. et al. Assessment of the Biodistribution and Metabolism of 5-Fluorouracil as Monitored by 18 F PET and 19 F MRI: A comparative animal study. Nuclear Medicine and Biology, 23: , Novikoff AB. Some aspects of hepatoma NK. J Natl Cancer Inst. 15(5, Suppl.): , Huu D et al. Persistent infection of a rat kidney cell with Rauscher murine leukemia virus. J Bacteriol 92: , Chen G et al. Determination of intracellular reduced gluthadione and glutathione related enzyme activities in cisplatin-sensitive and resistant experimental ovarian carcinoma cell s. Cancer Lett 46: , Greene LA et al. Establishment of a noradrenergic clonal of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci USA 73: , Eccleston E et al. Basophilic leukemia in the albino rat and a demonstration of the basopoietin. Nat New Biol 244: 73-6, Richter KH et al. A system for the study of epidermal G1-chalone in cell culture. The rat tongue epithelia cell RTE-2. Exp Cell Res 150(1): 68-76, Maligne Tumoren, Entstehung - Wachstum - Chemotherapie, 1981, Editio Cantor, D-7960 Aulendorf. ISBN Wintzerith M et al. Comparative study of free uridylic nucleotides in the normal liver, the regenerating liver and in the Zajdela hepatoma. CR Seances Soc Biol Fil 156: , the cellosaurus represents a detailed data collection bank of a plethora of cell relevant data from various cell banks. All of the products listed in Table 1/Table 2 are intended for research use only, not for use in human, therapeutic or diagnostic applications. The General Terms and Conditions of Supply of CLS Cell Lines Service GmbH are valid. According to the Terms, the products are not intended to be resold and/or modified for resale. Their usage in order to provide commercial services or to manufacture commercial products is prohibited unless approved in writing by CLS. Please contact service@clsgmbh.de if you have further questions or concerns. info@clsgmbh.de website: Page 6 of 6
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