Cell Therapy: The Future Of Medicine?

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1 Cell Therapy: The Future Of Medicine? Nada Alaaeddine, Director of Regenerative and dinflammation Lab Associate professor Faculty de Medicine, USJ

2 Why stem cells?

3 A Mother Cell different from all other cells. stem cell SELF RENEWAL (copying) DIFFERENTIATION (specializing) stem cell specialized cell e.g. muscle cell, nerve cell

4 A Mother Cell different from all other cells The medicinal signaling gcell Paracrine Effect

5 Diseases Treated by Stem Cells 1. Hepatic Cirrhosis 2. Cardiovascular disorders ex Berger's disease 3. osteoarthritis hii 4. Osteogenesis imperfect 5. Alzheimer 6. Parkinson 7. Muscular Dystrophy 8. Chronic renal insufficiency 9. Chronic obstructive pulmonary disease 10. Diabetes type I and II 11. Macular Degeneration 12. Retinitis pigmentosa 13. The corneal ulcer

6 OUR LABORATORY

7 Isolation of Mesenchymal stem cells First lab to OPTIMISE the isolation of stem cells from fat PROVE that those cells are really stem cells means they fit the criteria of the international society of cellular therapy Adherence to plastic Cellular markers Differentiation for THERAPY WE NEED A HIGH NUMBER OF CELLS SO we need to proliferate and to prove after proliferation that the cells are still stem cells

8 Immunophénotypage des cellules souches mésenchymateuses Adipose tissue derived mesenchymal stem cells Umbilical Cord mesenchymal stem cells Bone marrow mesenchymal stem cells Les A-MSCs, les B- MSCs et les C-MSCs expriment les marqueurs de surface CD105, CD73, CD90, mais n'expriment pas les marqueurs de surface CD34 et CD14.

9 Caractérisation des A-MSC, B-MSC, et C-MSC Morphologie fibroblastique Capacité de différenciation

10 THE CHALENGE.. for THERAPY A HIGH NUMBER OF CELLS IS REQUIRED Thus The need to proliferate and to prove after proliferation that the cells are still stem cells

11 Adherence and morphology after proliferation Day 7 Day 21

12 Markers of stem cells after proliferation CD 73 CD 29 CD 44 CD 73 CD 29 CD 44 SVF and ADSC were positive for CD 29, CD 44, CD 73, CD 90, and CD 105 CD 90 CD 105 CD 90 CD 105

13 CD 45 CD 31 They were negative CD 45 CD 31 for CD 31, CD34, CD45 and CD 106 CD 106 CD 34 CD 106 CD 34 When we proliferated stem cells they kept their characteristic

14 DIFFERENTIATION capacity after proliferation Adipogenic differentiation Osteogenic differentiation Chondrogenic differentiation Lipid vesicles Oil REd Calcium deposit alizarein red Detection of aggregan Alcian blue

15 Projets 1-An Evaluation of the Stemness, Paracrine, and Tumorigenic Characteristics of Highly Expanded, Minimally Passaged Adipose-Derived Stem Cells. Isolation and characterization of AD-MSCs Paracrine effect of AD-MSCs during passages Expression of tumor suppressor genes, oncogenes and ALDH. Cancer

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17 Cosmetics clinical trials BURNS Hypertrophic Scars We choose conditions where conventional methods like surgery,lasers, etc HAS NOT GIVEN SATISFACTORY results Stretch Marks (Striae) Non Healing Ulcers Atrophic Scars

18 Our case on Becker muscular dystrophy : Injection of stem cells in the muscle and intravenously. Did we IMPROVE muscle weakness?

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20 Effect of mesenchymal stem cells on Osteoarthritis: in vitro Chondrocytes Chondrocytes +MSC The expression of inflammation markers and proteases The expression of tissue inhibitors metalloproteases and anti inflammatory The expression of tissue inhibitors metalloproteases and anti inflammatory cytokines.

21 mm1 Effect of Stem cells and PRP on osteoarthritis Ch ctr Ch +5%PRP Increase: Chondrocytes proliferation Autophagy Anti inflammatory cytokines Cartilage regeneration Ch +10%PRP Ch +20%PRP Decrease: Inflammation Cartilage degradation Chondrocytes apoptosis

22 Slide 32 mm1 mayssam moussa, 5/3/2017

23 Our clinical case Injection of stem cells in the shoulder of the famous basketball player GARNETT

24 THE SCIENTIFIC PROOF MRI Of the Basketball Player Garnett de l épaule avant et six mois après l injection I i h hi k f h Increase in the thickness of the cartilage REGENERATION

25 Effect of AD-MSCs on Hepatocellular Carcinoma HEPG2 HEPG2+AD MDC Reduction of the expression of cancer markers AFP et DCP Reduction of inflammation Reduction of cancer invasion

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27 The Therapeutical Potential of Mesenchymal Stem Cells on the Amelioration of the Sperm Parameters in Mice under Oxidative Stress

28 So are we heading towards thefuture oftherapies?? IF YES

29 Topics to be addressed 1. The therapeutic mechanism of action needs to be defined. 2. Wide range of toxicology studies. d d d h f l d ll 3. Proper standardization and characterization of transplanted cell preparations have not yet been achieved.

30 Barriers to Success A SUCCESSFUL STEM CELL THERAPY COULD BE A DISRUPTIVE TECHNOLOGY BECAUSE IT WOULD POTENTIALLY REPLACE A NUMBER OF MAJOR MOLECULAR PHARMACEUTICALS AND MEDICAL PROSTHESES

31 Stem cells : THE HOPE!! NONETHELESS research has proven efficacy of STEM CELLS as THERAPY FOR LOSS OF CARTILAGE SKIN LESIONS AND REJUVENATION AND until today stem cells Might be a cure for conditions which conventional medicines offers no solution.

32 I HAVE A WAY FOR YOU TO LIVE FOREVER