Product Datasheet. Caspase-3 Antibody - (active/cleaved) NB Unit Size: 0.05 ml. Store at -20C. Avoid freeze-thaw cycles.

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1 Product Datasheet Caspase-3 Antibody - (active/cleaved) NB Unit Size: 0.05 ml Store at -20C. Avoid freeze-thaw cycles. Publications: 16 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 8/29/2017 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB Caspase-3 Antibody - (active/cleaved) Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer Product Description Host 0.05 ml This product is unpurified. The exact concentration of antibody is not quantifiable. Store at -20C. Avoid freeze-thaw cycles. Polyclonal 0.05% Sodium Azide IgG Unpurified Neat whole antisera Rabbit Gene ID 836 Gene Symbol Species Specificity/Sensitivity Immunogen Product Application Details Applications CASP3 Human, Mouse, Rat, Gerbil Preferentially detects active caspase-3 (large subunit: ~14-21 kda, and small subunit: ~10 kda). However, it may also detect pro-caspase-3 (~32 kda) in some cell or tissue systems. Nuclear immunostaining of caspase-3 is considered be an indication of active/cleaved caspase-3. Recombinant catalytically active human caspase-3 protein was used as immunogen. Western Blot, Flow (Intracellular), Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation Recommended Dilutions Western Blot 1:1000-1:2000, Immunohistochemistry, Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000, Flow (Intracellular) Application Notes Page 1 of 4 v.20.1 Updated 8/29/2017 Preferentially detects active caspase-3 (large subunit: ~14-21 kda, and small subunit: ~10 kda). However, it may also detect pro-caspase-3 (~32 kda) in some cell or tissue systems. Nuclear immunostaining of caspase-3 is considered be an indication of active/cleaved caspase-3. Use in Flow Intracellular reported in scientific literature (PMID )

3 Images Immunohistochemistry-Paraffin: Caspase-3 Antibody - (active/cleaved) [NB ] - Irradiated mouse spleen stained for Active/Cleaved Caspase-3 expression using NB at 1:2000. Staining is seen in the nuclei of a subset of the cell population. Caspase-3 expression in the nucleus is considered to be a marker of active/caspase-3 expression and apoptosis. Hematoxylin-eosin counterstain. Page 2 of 4 v.20.1 Updated 8/29/2017 Immunohistochemistry-Paraffin: Caspase-3 Antibody - (active/cleaved) [NB ] - Dog ischemic brain stained for Active/Cleaved Caspase-3 expression using NB at 1:2000. Staining is seen in the nuclei of dying neurons (black arrow) but not in the morphologically normal nuclei (open arrows). Caspase-3 expression in the nucleus is considered to be a marker of active/caspase-3 expression and apoptosis. Hematoxylin-eosin counterstain.

4 Publications Bozec A, Zangari J, Butori-Pepino M, Ilie M et al. MiR-223-3p inhibits angiogenesis and promotes resistance to cetuximab in head and neck squamous cell carcinoma Oncotarget 2017 Aug 22 [PMID: ] (IHC, Human) Mytych J, Wos I, Solek P, Koziorowski M. Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes. Exp Cell Res 2017 Jan 15 [PMID: ] (Human) Turtle JD, Strain MM, Aceves M et al. Pain Input Impairs Recovery after Spinal Cord Injury: Treatment with Lidocaine. J Neurotrauma 2017 Mar 15 [PMID: ] Mytych J, Romerowicz-Misielak M, Koziorowski M. Long-term culture with lipopolysaccharide induces dosedependent cytostatic and cytotoxic effects in THP-1 monocytes. Toxicol In Vitro. Mar :00AM [PMID: ] Solek P, Majchrowicz L, Bloniarz D et al. Pulsed or continuous electromagnetic field induce p53/p21-mediated apoptotic signaling pathway in mouse spermatogenic cells in vitro and thus may affect male fertility. Toxicology. Mar :00AM [PMID: ] (WB, Mouse) Phua T, Sng MK, Tan EH et al. Angiopoietin-like 4 Mediates Colonic Inflammation by Regulating Chemokine Transcript Stability via Tristetraprolin. Sci Rep. Mar :00AM [PMID: ] (Flow, Mouse) Sidhar H, Giri RK. Induction of Bex genes by curcumin is associated with apoptosis and activation of p53 in N2a neuroblastoma cells. Sci Rep. Feb :00AM [PMID: ] (WB, Mouse) Corsetti G, Romano C, Stacchiotti A et al. Endoplasmic Reticulum Stress and Apoptosis Triggered by Sub-Chronic Lead Exposure in Mice Spleen: a Histopathological Study. Biol Trace Elem Res. Dec :00AM [PMID: ] (Mouse) Li T, Sun KJ, Wang HD et al. Tert-butylhydroquinone Ameliorates Early Brain Injury After Experimental Subarachnoid Hemorrhage in Mice by Enhancing Nrf2-Independent Autophagy. Neurochem. Res Sep 01 [PMID: ] (WB, Mouse) Aulakh Gurpreet K, Balachandran Yadu, Liu Lixin, Singh Baljit. Angiostatin inhibits activation and migration of neutrophils. Cell Tissue Res Feb [PMID: ] Garraway SM, Woller SA, Huie JR et al. Peripheral noxious stimulation reduces withdrawal threshold to mechanical stimuli after spinal cord injury: Role of tumor necrosis factor alpha and apoptosis. Pain Aug 29 [PMID: ] Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int May 07 [PMID: ] (Flow-IC, Human) Details: Jurkat, Fig 9. More publications at Page 3 of 4 v.20.1 Updated 8/29/2017

5 Novus Biologicals USA 8100 Southpark Way, A-8 Littleton, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF008 NB7160 NBP H P02-2ug Goat anti-rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat anti-rabbit IgG (H+L) Secondary Antibody [HRP] Rabbit IgG Isotype Control Recombinant Human Caspase-3 Protein Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: