Pepsin Solution ready-to-use

Transcription

1 SIE HABEN DIE VISION, WIR HABEN DIE SUBSTANZ. Pepsin Solution Single component Pepsin Solution: only one component refrigerator stable Pepsin is a commonly used digestive enzyme for immunohistochemical (IHC) and in situ hybridization (ISH) procedures. Most of the commonly used fixatives as e. g. formamide cause cross-linking of proteins, which leads to masking or denaturation of tissue antigens. Treatment of fixed tissue sections with proteolytic enzymes as e.g. pepsin is often required to unmask antigens for optimal staining in IHC procedures. For ISH applications treatment with proteolytic enzyms is required to digest excess aldehyde linkages to enable DNA or RNA probes to penetrate the tissue sections. Ready-to-use pepsin solution in stabilizing buffer. Prod. S S S No. Pepsin Solution - 50 ml Pepsin Solution ml Pepsin Solution ml

2 AEC Solution ISH Powerful chromogenic substrate solution for HRP in in situ hybridization (ISH) procedures: only one component very strong signals very low background refrigerator stable A E C ( 3 - A m i n o Ethylcarbazole) is a commonly used chromogene for immunohistochemical (IHC) and in situ hybridization (ISH) procedures. Upon reaction with peroxidase AEC produces a red colored deposit that is soluble in alcohol. Thus, aqueous counterstain and mounting media must used. be Ready-to-use AEC solution in stabilizing buffer. Contains hydrogen peroxide. Optimized for the use in insitu hybridization (ISH) procedures to result in intensive staining with low background. Ig-λ detection on tonsil section stained with AEC Solution ISH (200x). Ig-λ detection on tonsil section stained with AEC Solution ISH (400x). S S AEC Solution ISH ml AEC Solution ISH ml

3 AEC Solution IHC Chromogenic substrate solution for HRP in immunohistochemistry procedures: only one component very strong signals very low background refrigerator stable A E C ( 3 - A m i n o Ethylcarbazole) is a commonly used chromogene for immunohistochemical (IHC) procedures supplied in one liquid component. In the presence of horseradish peroxidase (HRP) AEC Solution IHC deposits an intense red precipitate. The reaction product is soluble in alcohols and xylene and, therefore, aqueous counterstain and mounting media must be used. Ready-to-use AEC Solution IHC in stabilizing buffer. Contains hydrogen peroxide. Optimized for the use in immunohistochemical (IHC) procedures to result in intensive staining with low background. CD20-antibody-detection on tonsil section stained with novel AEC solution IHC. Ki-67-antibody-detection on tonsil section stained with novel AEC solution IHC. S S S AEC Solution IHC - 10ml AEC Solution IHC - 100ml AEC Solution IHC ml

4 DAB Solution Set 2 components DAB Solution Set (2 components) : optimized for in situ hybridization (ISH) and immunohistochemical (IHC) procedures refrigerator stable EBV ISH detection on lymph node (FFPE) stained with DAB Solution Set. DAB (3,3 -diaminobenzidine) is a widely used chromogen in immunohistochemical and immunoblotting applications. The DAB Solution Set is supplied as two liquid components, the buffer and the refrigerator stable chromogen solution, which are mixed together before use. Stability of the working solution is at least 6 hours at room temperature. In the presence of horseradish peroxidase (HRP) DAB deposits an intense brown precipitate. The reaction product is insoluble in alcohols and xylene and, therefore, a variety of counterstains and permanent mounting media can be used with DAB. The DAB Solution Set provides a two component liquid substrate system that deposits an intense brown specific stain in the presence of HRP. Immunohistochemical Ki-67 detection on tonsil (FFPE) stained with DAB Solution Set. Immunohistochemical Cytokeratin 5/6 detection on prostate (FFPE) stained with DAB Solution Set. S S DAB Solution Set ml DAB Solution Set ml

5 Mayer s Hematoxylin Solution Ready-to-use Mayer`s Hematoxylin Solution in stabilizing buffer: one stable component optimized for immunohistochemical (IHC) procedures non-toxic Mayer s Hematoxylin Solution is a staining reagent for histological applications. Nuclei in stained sections will be a blue-violet color with crisp nuclear detail. This reagent is suitable for use with all chromogens commonly used in immunohistochemical applications. (IHC) Ready-to-use Mayer s Hematoxylin Solution in stabilizing buffer. Does not contain chloralhydrate. Optimized for the use in immunohistochemical (IHC) procedures. Counterstain with Mayer`s Hematoxylin Solution on tonsil section. S S Mayer`s Hematoxylin Solution - 20 ml Mayer`s Hematoxylin Solution ml

6 one4all-antibody Stabilizer one4all-antibody Stabilizer: long term stabilizer for HRP and AP-conjugated antibodies The one4all-antibody Stabilizer is a unique stabilizer for all antibody-enzyme conjugates routinely used in immunohistochemistry (IHC) and in situ hybridization (ISH) applications as well as in ELISA. The activity of enzymes like horseradish peroxidase (HRP), alkaline phosphatase (AP), and beta-galactosidase (b-gal) is maintained over time at various storage temperatures when diluted in one4all-antibody Stabilizer. Besides its superior stabilizing effects, in many applications use of the one4all- Antibody Stabilizer will allow to further dilute the antibody conjugate without loss of specificity or sensitivity. In some assays, the same signal intensity as well as signal-to-noise ratio was observed even after an additional 7-fold dilution! Moreover, the one4all- Antibody Stabilizer can be easily used for applications requiring two or more antibody-enzyme conjugates in only one solution. one4all-antibody Stabilizer for horseradish peroxidase-, alkaline phosphatase and betagalactosidase-conjugated to antibodies. The one4all- Antibody Stabilizer is supplied as a solution. Residual activity of HRP-, AP and -galconjugated antibodies diluted in one4all-antibody Stabilizer vs. commercially available stabilizers (AP and HRP) or Tris-HEPES/BSA ( -gal), determined with ELISA. S one4all-antibody Stabilizer 250ml S one4all-antibody Stabilizer 1000ml

7 Nuclear Fast Red Solution Ready-to-use Nuclear Fast Red Solution : optimized for immunohistochemical (IHC) procedures no dilution required prior to use Nuclear Fast Red Solution is designed for use as a counterstaining solution in immunohistochemistry (IHC) procedures using common chromogens like BCIP/NBT. Nuclei will be stained redpink. Cytoplasm will be yellow or unstained. Specimens counterstained with Nuclear Fast Red Solution should be dehydrated and mounted with a xylenebased mounting media. This counterstain is not compatible with aqueous mounting media. Nuclear Fast Red Solution contains a nuclear fast red stain (a.k.a. Kernechtrot) in stabilizing buffer. No dilution is required prior to use. Counterstain with Nuclear Fast Red Solution on tonsil section. S Nuclear Fast Red Solution ml

8 Nuclear Red Solution Ready-to-use Nuclear Red Solution : optimized for in situ hybridization (ISH) and immunohistochemical (IHC) procedures no dilution required prior to use optimized to avoid overstaining Nuclear Red Solution is a nuclear fast red (a.k.a. Kernechtrot) based reagent for Specimens counterstaining applications in in situ hybridization (ISH) and immunohistochemistry (IHC) procedures. Nuclear Red Solution (readyto-use) is optimized to avoid overstaining of nuclei and for in situ hybridization (ISH) and immunohistochemistry (IHC) procedures using common chromogens like BCIP/NBT. Nuclei will be stained light red-pink. Cytoplasm will be light yellow or unstained. counterstained with Nuclear Red Solution should be dehydrated and mounted with a xylene-based mounting media. This counterstain is not compatible with aqueous mounting media. Nuclear Red Solution contains a nuclear fast red stain in stabilizing buffer. No dilution is required prior to use. Counterstain with Nuclear Red Solution on tonsil section. S Nuclear Red Solution ml

9 Nuclear Blue Solution Ready-to-use Nuclear Blue Solution : optimized for in situ hybridization (ISH) and immunohistochemical (IHC) procedures no dilution required prior to use optimized to avoid overstaining Nuclear Blue Solution is a hematoxylin based reagent for counterstaining applications in in situ hybridization (ISH) and immunohistochemistry (IHC) procedures using common chromogens like AEC and DAB. Nuclear Blue Solution (readyto-use) is optimized to avoid overstaining of nuclei. Specimens counterstained with Nuclear Blue Solution can be mounted in aqueous or xylene-based mounting media depending on the chromogen that is used. Nuclear Blue Solution contains a hematoxylin stain in a stabilizing buffer. No dilution is required prior to use. Counterstain with Nuclear Blue Solution on tonsil section. Ig-λ detection stained with DAB. Nuclei will be stained light blue-violet. S Nuclear Blue Solution ml

10 COT-DNA, human (10mg/ml) COT-DNA, human: suppress cross-hybridizations in microarray and in situ hybridization (ISH) experiments The COT fraction of human genomic DNA predominantly consists of rapidly annealing repetitive sequences. The COT-DNA, human is prepared from human placental DNA by shearing, denaturing and reannealing under conditions that enrich these repetitive sequences. COT- DNA, human is suitable for eliminating background when performing hybridization experiments as e. g. microarray or in situ hybridizations. 10 mg/ml COT Human DNA in 10mM Tris-HCl, 1 mm EDTA, ph 7.5. Size range shows a medium of bps. S S S COT-DNA, human - 1 mg COT-DNA, human - 10 mg COT-DNA, human - 50 mg

11 HRP-Green Solution Set 2 components HRP-Green Solution Set : Unique 2 components green chromogen for peroxidase Stable working solution for at least 4 hours optimized for in situ hybridization (ISH) and immunohistochemical (IHC) procedures DNA ISH detection on conisat section stained with HRP-Green Solution Set (400x). The HRP-Green Solution Set is a new chromogen used in immunohistochemical and chromogenic in situ hybridization applications. The HRP- Green Solution Set is supplied as two liquid components, the buffer and the chromogen solution, which are mixed together before use. Stability of the working solution is at least 4 hours at room temperature. In the presence of horseradish peroxidase (HRP) HRP-Green Solution deposits an intense green precipitate. HRP-Green shows an excellent contrast with red alkaline phophatase subtrates. The reaction product is insoluble in alcohols and xylene and, therefore, a variety of counterstains and permanent mounting media can be used with HRP-Green. The HRP-Green Solution Set provides a two component liquid substrate system that deposits an intense green stain in the presence of HRP. Immunohistochemical Estrogenreceptor detection on mamma section stained with HRP-Green Solution Set (400x). Immunohistochemical Cytokeratin 5/6 detection on prostate section stained with HRP-Green Solution Set (400x). S S HRP-Green Solution Set ml HRP-Green Solution Set ml

12 NBT/BCIP Solution NBT/BCIP Solution (): one component dark blue/violet chromogen for alkaline phosphatase optimized for immunohistochemical and chromogenic in situ hybridization procedures Ig-kappa mrna ISH detection on tonsil section stained with NBT/BCIP Solution (400x). The NBT/BCIP Solution is a chromogen used in immunohistochemical and chromogenic in situ hybridization applications. In the presence of alkaline phophatase (AP) NBT/ BCIP Solution deposits a dark blue/violet precipitate. NBT/ BCIP shows an excellent contrast with red horseradish peroxidase substrates. The reaction product is insoluble in alcohols and xylene and, therefore, a variety of counterstains and permanent mounting media can be used with NBT/ BCIP. The NBT/BCIP Solution is a one component liquid substrate that deposits an intense blue stain in the presence of AP. Ig-kappa (red)/ig-lambda (blue) mrna ISH detection on tonsil section stained with NBT/BCIP Solution and AEC Solution ISH (400x). Immunohistochemical Cytokeratin 5/6 detection on prostate section stained with NBT/BCIP Solution (200x). S S NBT/BCIP Solution () ml NBT/BCIP Solution () ml

13 AP-Red Solution Set IHC 2 components AP-Red Solution Set IHC: 2 components red chromogen for alkaline phosphatase optimized for immunohistochemical procedures Immunohistochemical Cytokeratin 5/6 detection on prostate section stained with AP-Red Solution Set IHC (400x). The AP-Red Solution Set IHC is a chromogen used in immunohistochemical applications. The AP-Red Solution Set IHC is supplied as two liquid components, the buffer and the chromogen solution, which are mixed together before use. In the presence of alkaline phophatase (AP) AP-Red Solution deposits a bright fuchsin-red precipitate. AP-Red shows an excellent contrast with green horseradish peroxidase substrates. The reaction product is insoluble in alcohols and xylene and, therefore, a variety of counterstains and permanent mounting media can be used with AP-Red. The AP-Red Solution Set IHC provides a two component liquid substrate system that deposits an intense red stain in the presence of alkaline phosphatase. Immunohistochemical CD20 detection on tonsil section stained with AP-Red Solution Set IHC (200x). Immunohistochemical CD20 detection on tonsil section stained with AP-Red Solution Set IHC (630x). S S AP-Red Solution Set IHC ml AP-Red Solution Set IHC ml BIOMOL GmbH Waidmannstr Hamburg Germany info@biomol.de Fon: +49 (0) Fax: +49 (0)