About Omics Group conferences
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1 About Omics Group OMICS Group International through its Open Access Initiative is committed to make genuine and reliable contributions to the scientific community. OMICS Group hosts over 400 leading-edge peer reviewed Open Access Journals and organize over 300 International Conferences annually all over the world. OMICS Publishing Group journals have over 3 million readers and the fame and success of the same can be attributed to the strong editorial board which contains over eminent personalities that ensure a rapid, quality and quick review process.
2 About Omics Group conferences OMICS Group signed an agreement with more than 1000 International Societies to make healthcare information Open Access. OMICS Group Conferences make the perfect platform for global networking as it brings together renowned speakers and scientists across the globe to a most exciting and memorable scientific event filled with much enlightening interactive sessions, world class exhibitions and poster presentations Omics group has organised 500 conferences, workshops and national symposium across the major cities including SanFrancisco,Omaha,Orlado,Rayleigh,SantaClara,Chicag o,philadelphia,unitedkingdom,baltimore,sanantanio,dub ai,hyderabad,bangaluru and Mumbai.
3 2th International Conference and Exhibition on Lasers, Optics & Photonics Application of multiphoton microscopy in diagnosis and therapy of early cancer Jianxin Chen Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education Fujian Normal University, China September,
4 Multiphoton Microscopy (MPM) Theoretically Maria Goppert-Mayer proposed the concept of two-photon excited fluorescence in M Goppert-Mayer Göppert-Mayer M., Űber Elementarakte mit zwei Quantensprüngen (On elementary processes with two quantum steps), Ann. Phys. 401 (1931) Experimentally Watt W. Webb presented the first multiphoton images as late as W. W. Webb Winfried Denk, James H. Strickler and Watt W. Webb (Two-Photon Laser Scanning Fluorescence Microscopy ) Science, 248 (1990) 73-76
5 Multiphoton Microscopy (MPM) Multi-photon Excited Fluorescence Two-photon Three-photon Multi-harmonic generation Second-harmonic generation Third-harmonic generation
6 Advantages of Multiphoton Microscopy Intrinsic optical sectioning ability Deeper penetration depths in scattering tissue, Reduced overall specimen photodamage and photobleaching Single photon excitation (488 nm) Two photon excitation (900 nm) (From Web s Group)
7 Applications of Multiphoton Microscopy It has a wide variety of biological and clinical applications Gene expression Protein interactions Calcium concentrations Neural activity Disease diagnosis and treatment Optical biopsy
8 Applications of Multiphoton Microscopy Cancer is a leading cause of death around the world WHO estimates that without intervention 84 million people will die of cancer between 2005 and Early detection of cancer greatly increases the chances for successful treatment.
9 Applications of Multiphoton Microscopy Esophagus Stomach Small intestine Large intestine
10 Instrument in our lab Ti:sapphire laser and LSM 510 META Microscope
11 Applications of Multiphoton Microscopy To differentiate between normal and dysplastic tissues To differentiate between normal and cancerous tissues To monitor cancer progression To perform photoablation of preinvasive cancer cells
12 We examined if MPM has the potential to differentiate between normal and dysplastic epithelial tissues
13 Differentiating between normal and dysplastic cervical tissues Epithelium Basement Membrane Stroma Normal tissue Inflammatory tissue Displastic tissue Applied Physics Letters 97, , 2010
14 Differentiating between normal and dysplastic cervical tissues Applied Physics Letters 97, , 2010
15 Differentiating between normal and Barrett esophageal tissue Normal squamous epithelium Barrett s esophagus Arch Pathol Lab Med 138, 204, 2014
16 Differentiating between normal and Barrett esophageal tissue Normal squamous epithelium Connective tissues in lamina propria layer Arch Pathol Lab Med 138, 204, 2014
17 Differentiating between normal and Barrett esophageal tissue Barrett epithelium at the upper part of mucosa Barrett epithelium at the deeper part of mucosa Arch Pathol Lab Med 138, 204, 2014
18 We examined if MPM has the potential to differentiate between normal and cancerous epithelial tissues
19 Differentiating between normal and cancerous stomach tissue MPM image H&E image Comparison of MPM images and H&E images from normal mucosa and submucosa Gastrointestinal Endoscopy, 73, , 2011
20 Differentiating between normal and cancerous stomach tissue MPM image H&E image Comparison of MPM images and H&E images from cancerous mucosa and submucosa Gastrointestinal Endoscopy, 73, , 2011
21 Differentiating between normal and cancerous stomach tissue MPM image H&E image MPM image of the nest of cancer and the surrounding fibrous stroma. Surgical Endoscopy, 25, , 2011
22 Differentiating between normal and cancerous stomach tissue Epithelium cell boundary Gastric gland orientation The collagen area
23 Differentiating between normal and cancerous esophagus tissue Normal stroma Neoplastic stroma Representative multiphoton images of the human esophageal stroma Journal of Biomedical Optics Letters 14, , 2009
24 Differentiating between normal and cancerous esophagus tissue Indicating MPM Collagen area Elastin area has the ablitiy to differentiate normal and cancerous esophageal stroma Emission spectra Ratio of SHG/TPEF Journal of Biomedical Optics Letters 14, , 2009
25 We examined if MPM has the potential to monitor cancer progression
26 Monitoring cervical tumor progression Normal tissue Precancerous tissue Cancerous tissue Applied Physics Letters 96, , 2010
27 Monitoring cervical tumor progression Correlation value as a function of pixel distance. Quantitative characterization parameters derived from SHG imaging Quantitatively linking collagen alteration and epithelial tumor progression Applied Physics Letters 96, , 2010
28 Monitoring colorectal tumor progression Laser Phys. Lett. 11 (2014)
29 Monitoring colorectal tumor progression
30 Monitoring colorectal tumor progression Normal tissue precancerous tissue Cancerous tissue PLoS ONE 7(6): e38655, 2012
31 Monitoring colorectal tumor progression Normal tissue precancerous tissue Cancerous tissue Biomedical Optics Express 2, , 2011
32 We examined if the combination of multiphoton imaging and absorption has the capability to perform photoablation of preinvasive cancer cells
33 Photoablation of preinvasive cancer cells Applied Physics Letters, 100, , 2012
34 Photoablation of preinvasive cancer cells Applied Physics Letters, 100, , 2012
35 What is the future?
36 What is the future? Confocal endoscope (ecle) Probe-based confocal endomicroscopy (pcle) Confocal Laser Endomicroscopy platforms and equipment Techniques in Gastrointestinal Endoscopy (2010) 12, 90-99
37 Acknowledgements National Natural Science Foundation of China ( ) ( ) ( ) ( ) ( ) Program for Changjiang Scholars and Innovative Research Team in University (IRT1115) Program for New Century Excellent Talents in University (NCET ) Natural Science Funds for Distinguished Young Scholar in Fujian Province (2009J06031)
38 Acknowledgements J X Chen S M Zhuo X Q Zhu L Q Zhen X S Jiang N R Liu M Nathanson D Jain A Sheth S Xiong J Zhao L Lin J Yan
39 Thank you for your attention!
40 Let Us Meet Again We welcome all to our future group conferences of Omics group international Please visit:
About OMICS International
About OMICS International OMICS International through its Open Access Initiative is committed to make genuine and reliable contributions to the scientific community. OMICS International hosts over 700
About OMICS International
About OMICS International OMICS International through its Open Access Initiative is committed to make genuine and reliable contributions to the scientific community. OMICS International hosts over 700
About OMICS International
About OMICS International OMICS International through its Open Access Initiative is committed to make genuine and reliable contributions to the scientific community. OMICS International hosts over 700
About OMICS International
About OMICS International OMICS International through its Open Access Initiative is committed to make genuine and reliable contributions to the scientific community. OMICS International hosts over 700
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About OMICS International
About OMICS International OMICS International through its Open Access Initiative is committed to make genuine and reliable contributions to the scientific community. OMICS International hosts over 700
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