Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK The Princess Alexandra Hospital NHS Trust The Michael Letcher Department of Cellular Pathology The Princess Alexandra Hospital NHS Trust Hamstel Road Harlow Essex CM20 1QX Contact: Mrs Lisa Greenhalgh Tel: +44 (0) / ext Website: Testing performed at the above address only Site activities performed away from the locations listed above: Location details Activity Main Hospital Site Building 4 Location A10 &11 Mortuary Services: Body Receipt/Release, Cold Storage, Post-Mortem Facility Assessment Manager: FN Page 1 of 10

2 DETAIL OF ACCREDITATION HUMAN BODY TISSUE Tissue: formalin-fixed biopsy, excision, resection and postmortem specimens diagnosis Examination of tissues in order to identify or exclude morphological and cytological abnormalities for the purpose of diagnosis Supported by: Specimen dissection S Dissection LP General Cut Up procedures LP BMS Tissue Transfer LP Fixation LP Decalcification LP Macropath digital photography LP Xylene clearance LP Tissue processing using Leica Peloris, Leica ASP300, Thermoshandon Pathcentre, Sakura VIP6 Tissue Processing and maintenance of the tissue processors LP Tissue embedding using Sakura Tissue Tek and Leica EG1160 embedding centres Embedding LP Assessment Manager: FN Page 2 of 10

3 HUMAN BODY TISSUE (cont d) Formalin Fixed Paraffin wax blocks of processed tissue. Fresh/frozen tissue FFPE slides Intraoperative examination of tissues in order to identify or exclude morphological and cytological abnormalities for the purpose of diagnosis Demonstration of: Basophilic and eosinophilic structures Acid Mucins Acid and neutral mucins Amyloid Elastic Fibres and Connective Tissue Microtomy using Leica 2135, Leica 2235, and Microm HM325 microtomes. s General Introduction to routine histology LP Procedure for cutting paraffin sections LP Leica CM1900 Cryostat s LP H&E for frozen LF Macroscopic and Microscopic examination: Automated staining and coverslipping using Leica ST5020 and CV5030 Automated Haematoxylin and Eosin staining and coverslipping LP Manual staining methods: Alcian Blue LF Alcian Blue - PAS LF Congo Red LF Elastin Van Gieson LF Assessment Manager: FN Page 3 of 10

4 Demonstration of: (cont d) Hematopoietic cells Helicobacter pylori Reticulin fibres Gram positive and negative bacteria Fungi, melanin, glycogen and Pneumocystis carinii (PCP) Routine stain for all examination of all tissue components, nuclear detail Giemsa LF Giemsa for Helicobacter pylori LF Gordon and Sweets Reticulin LF Gram stain LF Grocott LF H&E Haematoxylin and Eosin LF Melanin Masson Fontana LF Connective Tissue Masson s Trichrome LF Basement membrane Fibrin connective tissue Hepatitis B surface antigens and copper associated protein Polysaccharides including glycogen, fungi and mucins Haemosiderin Amyloid Modified Hexamine Silver LF MSB - Martius Scarlet Blue LF Orcein LF PAS(D) LF Perl s LF Sirius Red LF Assessment Manager: FN Page 4 of 10

5 FFPE slides Demonstration of: (cont d) Mucin, Cryptococcus neoformans Mast cells Connective tissues, collagen Calcium Salts Mycobacterium spp including tuberculosis Immunohistochemistry to detect the following: High molecular weight cytokeratins; CK1,5,10 and 14. Pan cytokeratin marker Diagnosis of specific carcinomas Oncoprotein marker, B-cell lineage Epithelial cells Lung, endometrial and ovarian carcinomas Southgate s Mucicarmine LF Toluidine Blue LF HVG Haematoxylin Van Gieson LF Modified Von Kossa LF Ziehl-Neelsen (ZN) LF Documented in-house methods used in accordance with Leica s operating instructions for the use of Bond Max and Bond III automated immunostainers. s General introduction to Immunocytochemistry and use of equipment LP Incorporating the following antibodies: 34BE12 AE1/AE3 AFP BCL2 BerEp4 CA125 Assessment Manager: FN Page 5 of 10

6 Immunohistochemistry (cont d) Mesothelial cells T cell marker T cells, B cells in the mantle zone Burkitt s Lymphoma, Lymphoma panel Haematological malignancies Hodgkin s Lymphoma B-cell marker Follicular dendritic cells Lymphoma panel Reed-Sternberg cells Endothelial Marker Plasma cells Leucocyte common antigen Neural cell adhesion molecule Macrophages B-cell marker Mast cells and GIST Plasma cells Intestinal epithelial cells Carcinoembryonic antigen Neuroendocrine Calretinin CD3 CD5 CD10 CD11c CD15 CD20 (L26) CD21 CD23 CD30 CD34 CD38 CD45 (LCA) CD56 CD68 CD79a CD117 (c-kit) CD138 CDX2 CEA Chromogranin A Assessment Manager: FN Page 6 of 10

7 Immunohistochemistry (cont d) Squamous and epithelial cells Cytokeratin 5/6 Glandular, transitional epithelial cells Cytokeratin 7 Basal epithelial cells Cytokeratin 14 Epithelial cells Cytokeratin 19 Gastrointestinal epithelial cells Cytokeratin 20 Mantle cell lymphoma Intermediate filaments Cell adhesion molecule Epithelial membrane antigen Oestrogen receptors Lymphatic endothelium Trophoblasts Human epithelial receptors in breast cancer Melanosomes Immunoglobulin A Immunoglobulin D Immunoglobulin G Immunoglobulin M Immunoglobulin kappa light chains Immunoglobulin lambda light chains Cyclin D1 Desmin E-Cadherin EMA (Epithelial Membrane Antigen) ER (Oestrogen Receptor) Factor 8 (Von Willebrand Factor) Human Chorionic Gonadotrophin HER2 Human Epithelial Receptor 2 HMB45 Immunoglobulin A Immunoglobulin D Immunoglobulin G Immunoglobulin M Kappa light chains Lambda light chains Assessment Manager: FN Page 7 of 10

8 Immunohistochemistry (cont d) Breast ductal carcinoma Melanocytes Cell proliferation marker Broad range cytokeratins Monocytes Myoepithelial cells Neutrophils and monocytes Tumour suppressor gene Basal cell marker Prostatic Acid Phosphatase Placental Alkaline Phosphatase Plasma cells Progesterone receptors Prostate specific antigen Renal cell carcinoma Melanoma, neuroendocrine Myoepithelial and smooth muscle Neuroendocrine Follicular cells of thyroid Mammoglobin Melan A MIB-1 MNF116 Myeloperoxidase Myosin Neutrophil Elastase P53 P63 Prostatic Acid Phosphatase Placental Alkaline Phosphatase Plasma cell marker PR (Progesterone Receptor) PSA (Prostate Specific Antigen) RCC (Renal Cell Carcinoma) S100 SMA (Smooth Muscle Actin) Synaptophysin Thyroglobulin Thyroid transcription factor-1 TTF1 (Thyroid transcription factor 1) Mesenchymal marker Vimentin Assessment Manager: FN Page 8 of 10

9 FFPE tissue on glass slides Slides prepared in house as above Immunohistochemistry (cont d) Wilms tumour gene product Molecular techniques for the detection and identification of over-expression of the HER-2 Gene in breast tissue Morphological assessment and interpretation/diagnosis WT1 (Wilm s tumour) Fluorescent In-Situ Hybridisation using VYSIS HYBrite and Zeiss AxioImager FISH Microscope M1. s Procedure for FISH analysis LP Procedure for HER2 Fluorescence In Situ Hybridisation LP Using various Olympus and Nikon miroscopes Histology and non-gynae reporting MP Assessment Manager: FN Page 9 of 10

10 HUMAN BODY TISSUE/CELLS Fluids, brushings, imprints, fineneedle aspirations from a diverse range of body sites including CSF, ascitic fluid, pleural fluid, synovial fluid, sputum, Breast FNA, FNA from other sites, Serous Fluid / Cyst Fluid, Bronchial washings and brushings, urine, skin scrapes Synovial fluid Slides prepared in house as above Cytopathological (Non-Gynae) examination activities for the purposes of clinical diagnosis Examination of cellular material in order to identify or exclude morphological and cytological abnormalities Basophilic and eosinophilic structures Differentiation of cell types and cytological abnormalities Assessment of birefringent crystals of urate and pyruvate Morphological assessment and interpretation/diagnosis END Diagnostic cytology (non-gynae) preparation, screening and reporting using T2000 processor, Leica ST 5020 and CV 5030 stainer/coverslipper. s Operating procedures for the Thinprep 2000 system LP Preparation of non-gynae specimens on the T2000 processor LP Automated Staining and Coverslipping of Cytology Specimens LP Haematoxylin and Eosin LP Automated PAP stain LP Manual May-Grunwald Giemsa stain LP Using BX51 Dual view microscope Preparation of synovial fluids LP Using various Olympus and Nikon microscopes Histology and non-gynae reporting MP Assessment Manager: FN Page 10 of 10

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