A new tool for screening : Cellular imaging (HCA/HCS)

Size: px
Start display at page:

Download "A new tool for screening : Cellular imaging (HCA/HCS)"

Transcription

1 Biotechnology company specialized in cellular imagery which provides HCA/HCS services A new tool for screening : Cellular imaging (HCA/HCS) Applications : Efficacy & Toxicoloxy assays Julian BURSZTYKA, PhD Nathalie MAUBON, PhD

2 Plan Company Context Applications Services offering 2

3 Plan Company Context Applications Services offering 3

4 HCS Pharma Offices a Laboratories in Rennes (Biopôle) dedicated to : - Cell culture - Automation : multiprobe (Perkin), ALH 3000 (Caliper) - Imaging: BD Pathway, Operetta Caliper ALH , 96 & 384-channel & gripper Multiprobe IIex Perkin 4-channel independent CIR * Authorisation BD Pathway 855 CIR = Crédit Impôt Recherche = Research Tax Credit 4

5 Strenghthened links with the University of Rennes Platform Access -MRIc : Photonic Microscopy, Transmission Electron Microscopy (TEM)... Confocal Leica SP8 SP5 Confocal olympus Video DMRIB - H2P2 : histopathologie & cytologie Laser Microdissection Automation of markings Scan histologie 5

6 Partnerships with research units of the University of Rennes UMR 6290 CNRS Université de Rennes 1 Specialists of interactions UV/skin, melanoma study 6

7 Plan Company Context Applications Services offering 7

8 Cellular Imaging: Increase in information vs. classical in vitro assays Classical in vitro assays (HTS) Plate reader +/- cell culture Cellular Imaging (HCS) Imager + cell culture Resuts A1 354 A A3 214 A4 143 Count WL1 WL2 WL3 size A A A A One value per well (absorbance, fluorescence, luminescence, etc.) Few information on biological content No measure on individual cell Multiples values per well, means on populations, statistics on individual cells Information on biological content : number of cells, sizes, shapes Identification cell by cell, measures of subpopulations and identification of rare cell events

9 Cell Imaging Benefits Highly visual Multiparametric Nucleï Co-culture neurons / macrophages Mitochondria Screening / Phenotypic analysis ROS Enriched information on biological content Primary Keratinocytes 9

10 Cellomic place in preclinical studies Cellomic (Phenotypic screening) Genomic Transcriptomic Proteomic Study of genes Study of ARN Study of proteins * * Study of cells * Studies of proteins by proteomic allow to identify a lot of proteins but do not give information on their localisation or activity. Phenotyping screening allow up to 3 markers but the Imaging Technology can bring the informtions on localisation and activity. Other advantages of cellomic: - In genomic studies, genes are not always the same level of expression - In transcriptomic studies, ARN activity is not always understood 10

11 Quantification by image analysis Image Segmentation: detection of regions of interest Analysis : intensity, surfaces, textures, colocalisation, translocation, Quantification 11

12 Plan Company Context Applications Services offering 12

13 HCS applications Dermatology/Pharmacology : Phenotypic screening & content screening : potentially in the same experiment (Up to 4 simultaneous markings) Phenotypic Screening : Cell cycle analysis, apoptosis, morphology, texture, measurement of ROS, mitochondrial viability Content screening : induction, phosphorylation, translocation of a targeted protein, colocalization of two targets 13

14 HCS applications Toxicology/Safety : Neogenesis Neurites aspect Double strand breaks, micronucleï UV Protection, moisturizing, redness Cardiotoxicity assays Co-cultures, 3D Primary cells, cell lines Steatosis, phospholipidosis 14

15 HCS applications Practical examples : UV protection (Measurement of ROS after UV-irradiation) Moisturizing (Measurement of AQP3 in the membrane of keratinocytes) Anti cellulite (inhibition of the differentiation of 3T3 cells in adipocytes) Customized experiments in close partnership 15

16 Genotoxicity : g-h2ax (Double-stranded DNA break marker) on human primary keratinocytes Etoposide CTRL DMSO Hoechst P-Histone H3 g-h2ax Merge CTRL Etoposide 16

17 Genotoxicity : g-h2ax (Double-stranded DNA break marker) on human primary keratinocytes Etoposide CTRL DMSO Quantification : Upstream screening and early removal of toxic assets for R&D of active ingredients 17

18 Genotoxicity : Comet Assay Development of a high throughput assay: 18

19 General toxicity : Steatosis & phospolipidosis 48h of treatment on HepG2 cell lines CTRL cells Rifampicin Cyclosporin A Sertraline Amiodarone Hoechst (blue), steatosis (green), phospholipidosis (red) Steatosis Phospholipidosis Cell Count 19

20 Measure of Aquaporine 3 (moisturizing parameter) on primary human keratinocytes Primary human keratinocytes undergoing differentiation Differentiated primary human keratinocytes In blue: Nucleï (hoechst), In yellow: aquaporin 3 (ICC-IF) Benchmark inducer = glyceryl glucoside - + Quantification

21 Antioxidant protection on primary human keratinocytes NAC - + Menadione CTRL DMSO NAC = N-acetylcystein Menadione Menadione+ NAC Ctrl + 0,5% DMSO ROS (Inducing Factor) 1,99 1,42 1 NAC protecting factor = 57,7% 100 µm Menadione alone or with 50 µm N-acetylcystein, n = 6 ROS = Reactive Oxygen Species In development: UV-Induced cell protection 21

22 Antiinflammatory effect on primary human keratinocytes In development: Inhibition of nuclear translocation of NF-kB, Inhibition of Nitric Oxid production, IL-8 Primary human keratinocytes Blue : Hoechst stained nucleï Green : NF-kB/P65 (ICC-IF) 22

23 Plan Company Context Applications Services offering 23

24 Typical assay Always Includes at least one cell count (toxicity) Includes the quantification of one or more endpoints of interest. = Multi-parametric assays - Free choice of number of repliquas and concentrations - Test of actives in mixes (Only one requirement = solubility) 24

25 Services offering Qualified models, routine studies Custom-models developement CIR authorisation 25

26 Thanks for your attention HCS Pharma: Expert assessment Efficiency Close partnership Transparency Acquisition & images analysis Experimentation Results & statistics Reporting and follow up Study design 26

27 CONTACTS Nathalie MAUBON PhD in biology CEO, CSO Julian BURSZTYKA PhD in toxicology COO HCS Pharma 6 Rue Pierre Joseph Colin RENNES Phone : Mail : cosmeto@hcs-pharma.com 27

High Content Imaging : Meaningful pictures for relevant results in dermocosmetology

High Content Imaging : Meaningful pictures for relevant results in dermocosmetology High Content Imaging : Meaningful pictures for relevant results in dermocosmetology We deliver High Content screening services with exhaustive, high quality and robust phenotypic data. Nathalie MAUBON,

More information

High throughput screening of nanotoxicity based on oxidative stress paradigm

High throughput screening of nanotoxicity based on oxidative stress paradigm December 11 28 High throughput screening of nanotoxicity based on oxidative stress paradigm Saji George Dr Andre Nel s lab Dept. of Medicine (Division of Nanomedicine) UCLA December 11 28 Growing nanotech

More information

Use of online cell counting for micronucleus and neurite outgrowth assays on IN Cell Analyzer 2000

Use of online cell counting for micronucleus and neurite outgrowth assays on IN Cell Analyzer 2000 GE Healthcare Application note 28-9673-96 AA IN Cell Analyzer 2000 Use of online cell counting for micronucleus and neurite outgrowth assays on IN Cell Analyzer 2000 Key words: IN Cell Analyzer online

More information

SolNanoTox. How the solubility of nanoparticles impacts on their behaviour and their potential toxicity? Valérie FESSARD

SolNanoTox. How the solubility of nanoparticles impacts on their behaviour and their potential toxicity? Valérie FESSARD SolNanoTox How the solubility of nanoparticles impacts on their behaviour and their potential toxicity? Valérie FESSARD Program between France and Germany Up to 42 months; from March 2014 up to August

More information

Ahtiainen et al., http :// /cgi /content /full /jcb /DC1

Ahtiainen et al., http ://  /cgi /content /full /jcb /DC1 Supplemental material JCB Ahtiainen et al., http ://www.jcb.org /cgi /content /full /jcb.201512074 /DC1 THE JOURNAL OF CELL BIOLOGY Figure S1. Distinct distribution of different cell cycle phases in the

More information

Fluorescence Microscopy

Fluorescence Microscopy Fluorescence Microscopy Imaging Organelles Mitochondria Lysosomes Nuclei Endoplasmic Reticulum Plasma Membrane F-Actin AAT Bioquest Introduction: Organelle-Selective Stains Organelles are tiny, specialized

More information

nature methods Organelle-specific, rapid induction of molecular activities and membrane tethering

nature methods Organelle-specific, rapid induction of molecular activities and membrane tethering nature methods Organelle-specific, rapid induction of molecular activities and membrane tethering Toru Komatsu, Igor Kukelyansky, J Michael McCaffery, Tasuku Ueno, Lidenys C Varela & Takanari Inoue Supplementary

More information

ROS Activity Assay Kit

ROS Activity Assay Kit ROS Activity Assay Kit Catalog Number KA3841 200 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials

More information

BV OSC BARNET. A Quasi Drug Whitening Benchmark. Superior Skin Penetration Compared to Other Whiteners Reduces Age Spots Great Stability in Formulas

BV OSC BARNET. A Quasi Drug Whitening Benchmark. Superior Skin Penetration Compared to Other Whiteners Reduces Age Spots Great Stability in Formulas BARNET CLARIFYING BV OSC A Quasi Drug Whitening Benchmark RO O O H OR CHCH 2 OR OR Superior Skin Penetration Compared to Other Whiteners Reduces Age Spots Great Stability in Formulas The information contained

More information

In vitro macrophage assays for selection of drug candidates for preclinical development. Philippa Allen GSK

In vitro macrophage assays for selection of drug candidates for preclinical development. Philippa Allen GSK In vitro macrophage assays for selection of drug candidates for preclinical development. Philippa Allen GSK Role of macrophages in the lung First line of host defence to inhaled matter. Regulate immune

More information

JCB. Supplemental material. Gu et al.,

JCB. Supplemental material. Gu et al., Supplemental material Gu et al., http://www.jcb.org/cgi/content/full/jcb.201010075/dc1 T H E J O U R N A L O F C E L L B I O L O G Y Figure S1. S1P directly induces actin assembly. Actin assembly at the

More information

Multi-Parameter Apoptosis Assay Kit

Multi-Parameter Apoptosis Assay Kit Multi-Parameter Apoptosis Assay Kit Catalog Number KA1335 5 x 96 assays Version: 05 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay...

More information

Lipid (Oil Red O) staining Kit

Lipid (Oil Red O) staining Kit Lipid (Oil Red O) staining Kit Catalog Number KA4541 1 Kit Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General Information... 4 Materials

More information

PHENOTYPIC DYNAMICS OF MICROGLIAL AND MONOCYTE-DERIVED CELLS IN GLIOBLASTOMA-BEARING MICE.

PHENOTYPIC DYNAMICS OF MICROGLIAL AND MONOCYTE-DERIVED CELLS IN GLIOBLASTOMA-BEARING MICE. SUPPLEMENTARY FIGURES, TABLES AND VIDEOS PHENOTYPIC DYNAMICS OF MICROGLIAL AND MONOCYTE-DERIVED CELLS IN GLIOBLASTOMA-BEARING MICE. Clément Ricard 1,2,3,4, Aurélie Tchoghandjian 2,4, Hervé Luche 5, Pierre

More information

QUANTIFICATION AND CHARACTERIZATION OF UVB-INDUCED MITOCHONDRIAL FRAGMENTATION IN NORMAL PRIMARY HUMAN KERATINOCYTES

QUANTIFICATION AND CHARACTERIZATION OF UVB-INDUCED MITOCHONDRIAL FRAGMENTATION IN NORMAL PRIMARY HUMAN KERATINOCYTES QUANTIFICATION AND CHARACTERIZATION OF UVB-INDUCED MITOCHONDRIAL FRAGMENTATION IN NORMAL PRIMARY HUMAN KERATINOCYTES Romain Jugé 1,2, Josselin Breugnot 2, Célia Da Silva 1, Sylvie Bordes 2, Brigitte Closs

More information

Chapter 2. Aims & Objectives

Chapter 2. Aims & Objectives 2.1. Statement of the problem: Earlier reports have shown ambiguous alteration of histone marks in response to DNA damage in asynchronized population of cells. These histone marks not only undergo dynamic

More information

Supplementary Figure 1: si-craf but not si-braf sensitizes tumor cells to radiation.

Supplementary Figure 1: si-craf but not si-braf sensitizes tumor cells to radiation. Supplementary Figure 1: si-craf but not si-braf sensitizes tumor cells to radiation. (a) Embryonic fibroblasts isolated from wildtype (WT), BRAF -/-, or CRAF -/- mice were irradiated (6 Gy) and DNA damage

More information

Supplementary materials

Supplementary materials Supplementary materials Chemical library from ChemBridge 50,240 structurally diverse small molecule compounds dissolved in DMSO Hits Controls: No virus added μ Primary screening at 20 g/ml of compounds

More information

GFP/Iba1/GFAP. Brain. Liver. Kidney. Lung. Hoechst/Iba1/TLR9!

GFP/Iba1/GFAP. Brain. Liver. Kidney. Lung. Hoechst/Iba1/TLR9! Supplementary information a +KA Relative expression d! Tlr9 5!! 5! NSC Neuron Astrocyte Microglia! 5! Tlr7!!!! NSC Neuron Astrocyte! GFP/Sβ/! Iba/Hoechst Microglia e Hoechst/Iba/TLR9! GFP/Iba/GFAP f Brain

More information

Nature Immunology: doi: /ni eee Supplementary Figure 1

Nature Immunology: doi: /ni eee Supplementary Figure 1 eee Supplementary Figure 1 Hyphae induce NET release, but yeast do not. (a) NET release by human peripheral neutrophils stimulated with a hgc1 yeast-locked C. albicans mutant (yeast) or pre-formed WT C.

More information

NLRX1: 5 -GCTCCATGGCTTAGAGCATC-3 (forward) 5 -AACTCCTCCTCCGTCCTGAT-3 (reverse) β-actin

NLRX1: 5 -GCTCCATGGCTTAGAGCATC-3 (forward) 5 -AACTCCTCCTCCGTCCTGAT-3 (reverse) β-actin NLRX1 β-actin 1 2 3 4 5 6 1 2 3 4 5 6 NLRX1 (667 bp) β-actin (523 bp) Supplementary Figure 1: Expression of NLRX1 in human cell lines. 1: HeLa, 2: HEK293T, 3: MCF-7, 4:Ramos, 5:Jurkat, 6: THP1. The following

More information

Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii

Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii María Lázaro-Díez, Itziar Chapartegui-González, Santiago Redondo-Salvo, Chike Leigh, David Merino, David San Segundo, Jesús

More information

C-Phycocyanin (C-PC) is a n«sjfc&c- waefc-jduble phycobiliprotein. pigment isolated from Spirulina platensis. This water- soluble protein pigment is

C-Phycocyanin (C-PC) is a n«sjfc&c- waefc-jduble phycobiliprotein. pigment isolated from Spirulina platensis. This water- soluble protein pigment is ' ^Summary C-Phycocyanin (C-PC) is a n«sjfc&c- waefc-jduble phycobiliprotein pigment isolated from Spirulina platensis. This water- soluble protein pigment is of greater importance because of its various

More information

High resolution structural evidence suggests the Sarcoplasmic Reticulum forms microdomains with Acidic Stores (lyososomes) in the heart.

High resolution structural evidence suggests the Sarcoplasmic Reticulum forms microdomains with Acidic Stores (lyososomes) in the heart. High resolution structural evidence suggests the Sarcoplasmic Reticulum forms microdomains with Acidic Stores (lyososomes) in the heart. Daniel Aston, Rebecca A. Capel, Kerrie L. Ford, Helen C. Christian,

More information

Input of proteomics in nanoparticles toxicology: the example of macrophages responses to mineral nanoparticles

Input of proteomics in nanoparticles toxicology: the example of macrophages responses to mineral nanoparticles Input of proteomics in nanoparticles toxicology: the example of macrophages responses to mineral nanoparticles CEA-Grenoble, irtsv/lcbm, CNRS UMR 5249 Thierry Rabilloud Catherine Aude-Garcia Véronique

More information

Grade of steatosis. group Case No. Supplementary Figure 1:

Grade of steatosis. group Case No. Supplementary Figure 1: a Supplementary Figure 1: b group Case No Grade of steatosis 15m AL 2746 NN 1 15m AL 2746 BN 1 15m AL 2638 2LN 3 15m AL 2638 2RN 3 12m AL 2640 RN 0 12m AL 2640 BN 1 12m AL 2640 LN 2 12m AL 2635 NN 2 12m

More information

Longitudinal tracking of single live cancer cells to understand cell cycle effects of the

Longitudinal tracking of single live cancer cells to understand cell cycle effects of the Longitudinal tracking of single live cancer cells to understand cell cycle effects of the nuclear export inhibitor, selinexor Joshua M. Marcus 1, Russell T. Burke 1, John A. DeSisto 1, Yosef Landesman

More information

Focus Application. Compound-Induced Cytotoxicity

Focus Application. Compound-Induced Cytotoxicity xcelligence System Real-Time Cell Analyzer Focus Application Compound-Induced Cytotoxicity Featured Study: Using the Time Resolving Function of the xcelligence System to Optimize Endpoint Viability and

More information

6. TNF-α regulates oxidative stress, mitochondrial function and autophagy in neuronal cells

6. TNF-α regulates oxidative stress, mitochondrial function and autophagy in neuronal cells 6. TNF-α regulates oxidative stress, mitochondrial function and autophagy in neuronal cells 6.1 TNF-α induces mitochondrial oxidative stress in SH-SY5Y cells. The dysregulation of mitochondria and oxidative

More information

IP: anti-gfp VPS29-GFP. IP: anti-vps26. IP: anti-gfp - + +

IP: anti-gfp VPS29-GFP. IP: anti-vps26. IP: anti-gfp - + + FAM21 Strump. WASH1 IP: anti- 1 2 3 4 5 6 FAM21 Strump. FKBP IP: anti-gfp VPS29- GFP GFP-FAM21 tail H H/P P H H/P P c FAM21 FKBP Strump. VPS29-GFP IP: anti-gfp 1 2 3 FKBP VPS VPS VPS VPS29 1 = VPS29-GFP

More information

ICNIRP 7th International NIR Workshop

ICNIRP 7th International NIR Workshop SOMETHING NEW FROM EXPERIMENTAL STUDIES? ISABELLE LAGROYE ICNIRP Member of SC II École Pratique des Hautes Études, Bioelectromagnetics laboratory, Pessac France METHOD 2010, 2011, and 2012 peer-reviewed

More information

A. Generation and characterization of Ras-expressing autophagycompetent

A. Generation and characterization of Ras-expressing autophagycompetent Supplemental Material Supplemental Figure Legends Fig. S1 A. Generation and characterization of Ras-expressing autophagycompetent and -deficient cell lines. HA-tagged H-ras V12 was stably expressed in

More information

Muse Assays for Cell Analysis

Muse Assays for Cell Analysis Muse Assays for Cell Analysis Multiple Assay Outputs for Cell Analysis Cell Health Cell Signalling Immunology Muse Count & Viability Kit Muse Cell Cycle Kit Muse Annexin V & Dead Cell Kit Muse Caspase

More information

Prediction of invasiveness of hepatic tumor cells

Prediction of invasiveness of hepatic tumor cells Prediction of invasiveness of hepatic tumor cells (Overexpression of Romo1 Promotes Production of Reactive Oxygen Species and Invasiveness of Hepatic Tumor Cells) (Romo1 : Reactive Oxygen Species Modulator

More information

Supplementary Information. Detection and delineation of oral cancer with a PARP1 targeted optical imaging agent

Supplementary Information. Detection and delineation of oral cancer with a PARP1 targeted optical imaging agent Supplementary Information Detection and delineation of oral cancer with a PARP1 targeted optical imaging agent Authors: Susanne Kossatz a, Christian Brand a, Stanley Gutiontov b, Jonathan T.C. Liu c, Nancy

More information

Sensitivity of mammalian cells to higher concentrations of reactive oxygen species induced by radiation or chemical treatment

Sensitivity of mammalian cells to higher concentrations of reactive oxygen species induced by radiation or chemical treatment Sensitivity of mammalian cells to higher concentrations of reactive oxygen species induced by radiation or chemical treatment The reactive oxygen species (ROS) are group of very unstable compounds that

More information

Supplementary Figure 1.

Supplementary Figure 1. Supplementary Figure 1. Visualization of endoplasmic reticulum-mitochondria interaction by in situ proximity ligation assay. A) Illustration of targeted proteins in mitochondria (M), endoplasmic reticulum

More information

AN ACTIVE SHELTER AGAINST POLLUTION V.16

AN ACTIVE SHELTER AGAINST POLLUTION V.16 AN ACTIVE SHELTER AGAINST POLLUTION V.16 THE SKIN IS CONSTANTLY EXPOSED The skin is an interface and thus is constantly exposed to environmental agents: Sun radiation Smoke Air pollution Free radicals

More information

Focus Application. Compound-Induced Cytotoxicity

Focus Application. Compound-Induced Cytotoxicity xcelligence System Real-Time Cell Analyzer Focus Application Compound-Induced Cytotoxicity For life science research only. Not for use in diagnostic procedures. Featured Study: Using the Time Resolving

More information

Prolonged mitotic arrest induces a caspase-dependent DNA damage

Prolonged mitotic arrest induces a caspase-dependent DNA damage SUPPLEMENTARY INFORMATION Prolonged mitotic arrest induces a caspase-dependent DNA damage response at telomeres that determines cell survival Karolina O. Hain, Didier J. Colin, Shubhra Rastogi, Lindsey

More information

Anti-Apoptotic Effects of Cellular Therapy

Anti-Apoptotic Effects of Cellular Therapy Anti-Apoptotic Effects of Cellular Therapy Jason Lapetoda 1, Lee K Landeen, PhD 1, George K Michalopoulos, MD 2, Patricia W Bedard, PhD 1 1 Vital Therapies, Inc., San Diego, CA, USA 2 University of Pittsburgh

More information

McWilliams et al., http :// /cgi /content /full /jcb /DC1

McWilliams et al., http ://  /cgi /content /full /jcb /DC1 Supplemental material JCB McWilliams et al., http ://www.jcb.org /cgi /content /full /jcb.201603039 /DC1 THE JOURNAL OF CELL BIOLOGY Figure S1. In vitro characterization of mito-qc. (A and B) Analysis

More information

Supplementary Figure Legends

Supplementary Figure Legends Supplementary Figure Legends Supplementary Figure 1. Comparison of RNP IC-mediated NET formation. Quantification of DNA release induced by ICs consisting of SmRNP combined with SLE IgG 961 (n = 10), 1032

More information

Relative SOD1 activity. Relative SOD2 activity. Relative SOD activity (Infected:Mock) + CP + DDC

Relative SOD1 activity. Relative SOD2 activity. Relative SOD activity (Infected:Mock) + CP + DDC Supplementary Figure 1. SOD1 activity is significantly increased relative to SOD1 levels. SOD1 and SOD2 activities in the infected mork13 cells are shown normalised to their corresponding levels and relative

More information

Supplementary Information

Supplementary Information Nature Immunology doi:1.138/ni.2477 Supplementary Information Capillary and arteriolar pericytes attract innate leukocytes exiting through venules and instruct them with pattern recognition and motility

More information

bio-mof-1 DMASM Wavenumber (cm -1 ) Supplementary Figure S1 FTIR spectra of bio-mof-1, DMASMI, and bio-mof-1 DMASM.

bio-mof-1 DMASM Wavenumber (cm -1 ) Supplementary Figure S1 FTIR spectra of bio-mof-1, DMASMI, and bio-mof-1 DMASM. bio-mof-1 Transmittance bio-mof-1 DMASM DMASMI 2000 1500 1000 500 Wavenumber (cm -1 ) Supplementary Figure S1 FTIR spectra of bio-mof-1, DMASMI, and bio-mof-1 DMASM. Intensity (a.u.) bio-mof-1 DMASM as

More information

Supplemental Data. Wang et al. (2013). Plant Cell /tpc

Supplemental Data. Wang et al. (2013). Plant Cell /tpc Supplemental Data. Wang et al. (2013). Plant Cell 10.1105/tpc.112.108993 Supplemental Figure 1. 3-MA Treatment Reduces the Growth of Seedlings. Two-week-old Nicotiana benthamiana seedlings germinated on

More information

INTRODUCTION. This material is quoted from below PDF and modified.

INTRODUCTION. This material is quoted from below PDF and modified. INTRODUCTION While drug response profiling of cancer cells in two-dimensional culture has been a mainstay of predictive biomarker discovery and anti-cancer drug development, there are aspects of tumor

More information

Identification of GLP1R agonists using a novel high throughput screening assay Wan Namkung, Ph.D.

Identification of GLP1R agonists using a novel high throughput screening assay Wan Namkung, Ph.D. Identification of GLP1R agonists using a novel high throughput screening assay Wan Namkung, Ph.D. College of Pharmacy, Yonsei University Contents High-throughput screening (HTS) HTS assays for identification

More information

Lysine analogue of Polymyxin B as a significant opportunity for photodynamic antimicrobial chemotherapy

Lysine analogue of Polymyxin B as a significant opportunity for photodynamic antimicrobial chemotherapy SUPPORTING INFORMATION Lysine analogue of Polymyxin B as a significant opportunity for photodynamic antimicrobial chemotherapy Florent Le Guern, Tan-Sothea Ouk *, Catherine Ouk, Regis Vanderesse +, Yves

More information

Serafino et al. Thymosin α1 activates complement receptor-mediated phagocytosis in human monocyte-derived macrophages. SUPPLEMENTARY FIGURES

Serafino et al. Thymosin α1 activates complement receptor-mediated phagocytosis in human monocyte-derived macrophages. SUPPLEMENTARY FIGURES Supplementary Fig. S1. Evaluation of the purity and maturation of macrophage cultures tested by flow cytometry. The lymphocytic/monocytic cellular fraction was isolated from buffy coats of healthy donors

More information

Supplementary Figure 1. Characterization of human carotid plaques. (a) Flash-frozen human plaques were separated into vulnerable (V) and stable (S),

Supplementary Figure 1. Characterization of human carotid plaques. (a) Flash-frozen human plaques were separated into vulnerable (V) and stable (S), Supplementary Figure 1. Characterization of human carotid plaques. (a) Flash-frozen human plaques were separated into vulnerable (V) and stable (S), regions which were then quantified for mean fluorescence

More information

Nature Structural & Molecular Biology: doi: /nsmb Supplementary Figure 1. Generation and validation of mtef4-knockout mice.

Nature Structural & Molecular Biology: doi: /nsmb Supplementary Figure 1. Generation and validation of mtef4-knockout mice. Supplementary Figure 1 Generation and validation of mtef4-knockout mice. (a) Alignment of EF4 (E. coli) with mouse, yeast and human EF4. (b) Domain structures of mouse mtef4 compared to those of EF4 (E.

More information

Translating Tissue Phenomics and Big Data Distillation to Clinical Histopathology

Translating Tissue Phenomics and Big Data Distillation to Clinical Histopathology International Symposium for Tissue Phenomics 2016 Translating Tissue Phenomics and Big Data Distillation to Clinical Histopathology Dr Peter Caie Senior Research Fellow QUAntitative and Digital (QUAD)

More information

Mechanisms of Cell Injury

Mechanisms of Cell Injury Causes of Cell Injury 1- oxygen deprivation (anoxia) 2- physical agents 3- chemical agents 4- infections agents 5- immunologic reactions 6- genetic defects 7- nutritional imbalances Mechanisms of Cell

More information

Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation

Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation Supplementary material; Title; Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation Authors; Petra Wäster 1, Ida Eriksson 1, Linda Vainikka 1, Inger Rosdahl 2,

More information

2D protein gel analysis tool for studying the protein phosphorylation changes associated with contraction and relaxation of vascular smooth muscle

2D protein gel analysis tool for studying the protein phosphorylation changes associated with contraction and relaxation of vascular smooth muscle 2D protein gel analysis tool for studying the protein phosphorylation changes associated with contraction and relaxation of vascular smooth muscle Internship details Where: Department of Bioengineering

More information

Pathogenesis of lupus apoptosis clearance and regulation of self tolerance. Durga Prasanna Misra

Pathogenesis of lupus apoptosis clearance and regulation of self tolerance. Durga Prasanna Misra Pathogenesis of lupus apoptosis clearance and regulation of self tolerance Durga Prasanna Misra Format Apoptosis Apoptosis in SLE Decreased apoptosis of autoreactive cells Increased apoptosis Defective

More information

the future demands for animal-

the future demands for animal- Nutrigenomics a tool to meet the future demands for animal- derived d foods Mark Gaffney, Ph.D. Alltech Bioscience Center, Ireland 23 rd June 2011 Are we ready for 9 billion??? The World needs to double

More information

Developing quantitative slide-based assays to assess target inhibition in oncology drug discovery and development

Developing quantitative slide-based assays to assess target inhibition in oncology drug discovery and development Developing quantitative slide-based assays to assess target inhibition in oncology drug discovery and development TIGA Workshop June 25-26, 2010 Doug Bowman Millennium Pharmaceuticals 2010 Millennium Pharmaceuticals

More information

Supplementary material. Supplementary Figure legends

Supplementary material. Supplementary Figure legends Supplementary material Supplementary Figure legends Supplementary Figure 1: Senescence-associated proliferation stop in response to oncogenic N-RAS expression Proliferation of NHEM cells without (ctrl.)

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION Advances in pancreatic islet monolayer culture on glass surfaces enable superresolution microscopy and insights into beta cell ciliogenesis and proliferation Edward A. Phelps,

More information

Rapid parallel measurements of macroautophagy and mitophagy in

Rapid parallel measurements of macroautophagy and mitophagy in Supplemental Figures Rapid parallel measurements of macroautophagy and mitophagy in mammalian cells using a single fluorescent biosensor Sargsyan A, Cai J, Fandino LB, Labasky ME, Forostyan T, Colosimo

More information

Differential Signalling and Kinetics of Neutrophil Extracellular Trap Release Revealed by Quantitative Live Imaging

Differential Signalling and Kinetics of Neutrophil Extracellular Trap Release Revealed by Quantitative Live Imaging Differential Signalling and Kinetics of Neutrophil Extracellular Trap Release Revealed by Quantitative Live Imaging Maarten van der Linden 1, Geertje H.A. Westerlaken 1, Michiel van der Vlist 1, Joris

More information

Supporting Information

Supporting Information Supporting Information Bauer et al. 10.1073/pnas.1610421113 SI Materials and Methods Immunofluorescence experiments were performed as described (8). Briefly, NUGC-3 cells were treated with 25 μm PK11007

More information

Figure S1, Beyer et al.

Figure S1, Beyer et al. Figure S1, eyer et al. Pax7 Myogenin si sitrl Hoechst T = 72h 14 1.8.6.4.2 12 1 8 6 4 2 24h 48h 96h diff. sitrl siset1 212 72h diff. b1 td r t Se km MyH Vinculin Myogenin β-ctin Vinculin MW b1 ka td r

More information

AXION PLATES INDUSTRY-LEADING 768 ELECTRODES AT THE THROUGHPUT YOU NEED AXION MEA PLATES

AXION PLATES INDUSTRY-LEADING 768 ELECTRODES AT THE THROUGHPUT YOU NEED AXION MEA PLATES AXION PLATES INDUSTRY-LEADING 768 ELECTRODES AT THE THROUGHPUT YOU NEED Your research requires insightful, high-quality data from microelectrode array (MEA) experiments. Axion BioSystems MEA plates meet

More information

Cell Damage. Standardized and automatic determination of DNA double strand breaks using immunofluorescence

Cell Damage. Standardized and automatic determination of DNA double strand breaks using immunofluorescence Standardized and automatic determination of DNA double strand breaks using immunofluorescence Patents: CN 2702421, EP 2208068, TR 201308237, 200880115751 The Platform Motorized, inverse fluorescence microscope

More information

Radiation induced DNA double strand breaks undergoing PET/CT examinations.

Radiation induced DNA double strand breaks undergoing PET/CT examinations. Radiation induced DNA double strand breaks undergoing PET/CT examinations. Poster No.: C-1685 Congress: ECR 2011 Type: Scientific Exhibit Authors: M. S. May, M. Brand, T. Löwe, M. Sommer, C. Engert, U.

More information

Supplementary Figure 1

Supplementary Figure 1 Supplementary Figure 1 Expression of apoptosis-related genes in tumor T reg cells. (a) Identification of FOXP3 T reg cells by FACS. CD45 + cells were gated as enriched lymphoid cell populations with low-granularity.

More information

Supporting Information

Supporting Information Copyright WILEY-VCH Verlag GmbH & Co. KGaA, 69469 Weinheim, Germany, 212. Supporting Information for Adv. Funct. Mater., DOI:.2/adfm.2122233 MnO Nanocrystals: A Platform for Integration of MRI and Genuine

More information

Katarzyna Równy. Combination of antioxidants, plant extract enhancing VDR synthesis and NB-UVB therapy for vitiligo skin care

Katarzyna Równy. Combination of antioxidants, plant extract enhancing VDR synthesis and NB-UVB therapy for vitiligo skin care Combination of antioxidants, plant extract enhancing VDR synthesis and NB-UVB therapy for vitiligo skin care Katarzyna Równy Scientific Specialist @ dr Irena Eris Centre for Science and Research Research

More information

Ultrapure laser-synthesized Si-based nanomaterials for biomedical. applications: in vivo assessment of safety and biodistribution

Ultrapure laser-synthesized Si-based nanomaterials for biomedical. applications: in vivo assessment of safety and biodistribution Supplementary Information Ultrapure laser-synthesized Si-based nanomaterials for biomedical applications: in vivo assessment of safety and biodistribution Tarek Baati, 1 Ahmed Al-Kattan, 2 Marie-Anne Esteve,

More information

Prof. Jean-Pierre Lepoittevin University of Strasbourg, France SCCS WG on methodology Luxemburg, July 1st 2015

Prof. Jean-Pierre Lepoittevin University of Strasbourg, France SCCS WG on methodology Luxemburg, July 1st 2015 Potency classification of skin sensitizers: development and use of in vitro methods Prof. Jean-Pierre Lepoittevin University of Strasbourg, France SCCS WG on methodology Luxemburg, July 1st 2015 Research

More information

Mitochondrial electron transport chain as a target for anti-cancer drugs

Mitochondrial electron transport chain as a target for anti-cancer drugs Mitochondrial electron transport chain as a target for anti-cancer drugs Jakub Rohlena Laboratory of Molecular Therapy, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prague, Czech

More information

Macrophages form functional vascular mimicry channels in vivo. SI Figures and Legend

Macrophages form functional vascular mimicry channels in vivo. SI Figures and Legend Macrophages form functional vascular mimicry channels in vivo Authors: *Faith H. Barnett, *Mauricio Rosenfeld, Malcolm Wood, William Kiosses, Yoshihiko Usui, Valentina Marchetti, Edith Aguilar, and Martin

More information

Supplementary Figure 1 Lymphocytes can be tracked for at least 4 weeks after

Supplementary Figure 1 Lymphocytes can be tracked for at least 4 weeks after Supplementary Figure 1 Lymphocytes can be tracked for at least 4 weeks after photoconversion by using H2B-Dendra2. 4-5 PPs of H2B-Dendra2 BM chimeras were photoconverted and analyzed 7 days (upper panel)

More information

Simultaneous Blood Brain Barrier Crossing and Protection for Stroke Treatment Based on Edaravone-Loaded Ceria Nanoparticles

Simultaneous Blood Brain Barrier Crossing and Protection for Stroke Treatment Based on Edaravone-Loaded Ceria Nanoparticles Supporting Information Simultaneous Blood Brain Barrier Crossing and Protection for Stroke Treatment Based on Edaravone-Loaded Ceria Nanoparticles Qunqun Bao, Ping Hu, * Yingying Xu, Tiansheng Cheng, Chenyang

More information

Set the stage: Genomics technology. Jos Kleinjans Dept of Toxicogenomics Maastricht University, the Netherlands

Set the stage: Genomics technology. Jos Kleinjans Dept of Toxicogenomics Maastricht University, the Netherlands Set the stage: Genomics technology Jos Kleinjans Dept of Toxicogenomics Maastricht University, the Netherlands Amendment to the latest consolidated version of the REACH legislation REACH Regulation 1907/2006:

More information

Reperfusion Injury: How Can We Reduce It?

Reperfusion Injury: How Can We Reduce It? MI/CAD: Practical Question in Management of AMI Patients Reperfusion Injury: How Can We Reduce It? Hyun-Jai Cho, M.D., Ph.D Cardiovascular Center & Department of Internal Medicine Seoul National University

More information

Mohammad Husain Department of Biotechnology, Jamia Millia Islamia New Delhi

Mohammad Husain Department of Biotechnology, Jamia Millia Islamia New Delhi Role of Vitamin D receptor (VDR) in HIV induced tubular injury Mohammad Husain Department of Biotechnology, Jamia Millia Islamia New Delhi 07/10/2015 INTRODUCTION Vitamin D is technically not a Vitamin;

More information

Supplementary Figure 1

Supplementary Figure 1 Supplementary Figure 1 Dox Cis Cam Pac 0 15 1 15 1 15 1 15 1 15 µmole/l Ub p53 Cytotoxic anticancer agents increase p53 levels but do not generally promote the accumulation of ubiquitinated. Western blots

More information

XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System

XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System Application Note Normalization XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System Authors Yoonseok Kam Kellie Chadwick Ned Jastromb Brian P. Dranka Agilent Technologies,

More information

Cinnamomum Essential Oil Prevents DNA Damage- Induced by Doxorubicin on CHO-K1 Cells

Cinnamomum Essential Oil Prevents DNA Damage- Induced by Doxorubicin on CHO-K1 Cells Indonesian Journal of Cancer Chemoprevention, 2017, 8(1): 27-31 ISSN: 2088 0197 Cinnamomum Essential Oil Prevents DNA Damage- Induced by Doxorubicin on CHO-K1 Cells Layung Sekar Sih Wikanthi, Nindi Wulandari,

More information

Clastogenic factors in blood plasma obtained from subjects exposed to. Carita Lindholm STUK - Radiation and Nuclear Safety Authority

Clastogenic factors in blood plasma obtained from subjects exposed to. Carita Lindholm STUK - Radiation and Nuclear Safety Authority Clastogenic factors in blood plasma obtained from subjects exposed to ionizing radiation Carita Lindholm STUK - Radiation and Nuclear Safety Authority Objectives: Identify clastogenic factors in blood

More information

J. Cell Sci. 129: doi: /jcs : Supplementary information

J. Cell Sci. 129: doi: /jcs : Supplementary information Movie 1. AgLDL is contained in small sub-regions of the lysosomal synapse that are acidic. J774 cells were incubated with agldl dual labeled with a ph sensitive and a ph insensitive fluorophore for 1 hr.

More information

Part-4. Cell cycle regulatory protein 5 (Cdk5) A novel target of ERK in Carb induced cell death

Part-4. Cell cycle regulatory protein 5 (Cdk5) A novel target of ERK in Carb induced cell death Part-4 Cell cycle regulatory protein 5 (Cdk5) A novel target of ERK in Carb induced cell death 95 1. Introduction The process of replicating DNA and dividing cells can be described as a series of coordinated

More information

Poorly soluble cobalt oxide particles trigger genotoxicity via multiple pathways

Poorly soluble cobalt oxide particles trigger genotoxicity via multiple pathways Uboldi et al. Particle and Fibre Toxicology (2016) 13:5 DOI 10.1186/s12989-016-0118-8 RESEARCH Open Access Poorly soluble cobalt oxide particles trigger genotoxicity via multiple pathways Chiara Uboldi

More information

Assay Name: Transwell migration using DAPI

Assay Name: Transwell migration using DAPI Assay Name: Transwell migration using DAPI Assay ID: Celigo_04_0001 Table of Contents Experiment: Identification of cells which have migrated through the membrane of a transwell insert....2 Celigo Setup...2

More information

What s in a Tipping Point?

What s in a Tipping Point? What s in a Tipping Point? Using Systems Biology to Characterize Adverse Oxidative Responses in Human Lung Cells Jenna M. Currier, Ph.D. ORISE Postdoctoral Fellow at U.S. EPA Mentors: Brian Chorley, Ph.D.

More information

Proteomic profiling of small-molecule inhibitors reveals dispensability of MTH1 for cancer cell survival

Proteomic profiling of small-molecule inhibitors reveals dispensability of MTH1 for cancer cell survival Supplementary Information for Proteomic profiling of small-molecule inhibitors reveals dispensability of MTH1 for cancer cell survival Tatsuro Kawamura 1, Makoto Kawatani 1, Makoto Muroi, Yasumitsu Kondoh,

More information

IKK-dependent activation of NF-κB contributes to myeloid and lymphoid leukemogenesis by BCR-ABL1

IKK-dependent activation of NF-κB contributes to myeloid and lymphoid leukemogenesis by BCR-ABL1 Supplemental Figures BLOOD/2014/547943 IKK-dependent activation of NF-κB contributes to myeloid and lymphoid leukemogenesis by BCR-ABL1 Hsieh M-Y and Van Etten RA Supplemental Figure S1. Titers of retroviral

More information

Spatially resolved multiparametric single cell analysis. Technical Journal Club 19th September 2017 Christina Müller (Group Speck)

Spatially resolved multiparametric single cell analysis. Technical Journal Club 19th September 2017 Christina Müller (Group Speck) Spatially resolved multiparametric single cell analysis Technical Journal Club 19th September 2017 Christina Müller (Group Speck) Why spatially resolved multiparametric single cell analysis? Multiparametric

More information

Supporting Information For

Supporting Information For Supporting Information For MicroRNA-Catalyzed Cancer Therapeutics Based on DNA-Programmed Nanoparticle Complex Xucheng Luo, 1 Zhi Li, 1 Ganglin Wang, 1 Xuewen He, 2,3 Xiaoqin Shen, 1 Quanhong Sun, 1 Li

More information

The Effects of Transposing Mitochondria From Drug Resistant to Drug Sensitive Breast Cancer Cells

The Effects of Transposing Mitochondria From Drug Resistant to Drug Sensitive Breast Cancer Cells The Effects of Transposing Mitochondria From Drug Resistant to Drug Sensitive Breast Cancer Cells By: Shahzaad Jahangier Department of Chemistry and Biochemistry University of Minnesota Duluth Breast Cancer

More information

Visual interpretation in pathology

Visual interpretation in pathology 13 Visual interpretation in pathology Tissue architecture (alteration) evaluation e.g., for grading prostate cancer Immunohistochemistry (IHC) staining scoring e.g., HER2 in breast cancer (companion diagnostic

More information

InhalT 90 Current Project Status

InhalT 90 Current Project Status InhalT 90 Current Project Status 90-Day Nose-Only Inhalation Toxicity Study of Cerium Oxide Nanoparticles in Female Wistar Rats with subsequent Analysis of Gene Expression Profiles Sponsor s Study Monitor

More information

Genotoxicity Testing Strategies: application of the EFSA SC opinion to different legal frameworks in the food and feed area

Genotoxicity Testing Strategies: application of the EFSA SC opinion to different legal frameworks in the food and feed area Genotoxicity Testing Strategies: application of the EFSA SC opinion to different legal frameworks in the food and feed area Juan Manuel Parra Morte. Pesticides Unit. EFSA. 19th Annual Conference of the

More information

Dominic J Smiraglia, PhD Department of Cancer Genetics. DNA methylation in prostate cancer

Dominic J Smiraglia, PhD Department of Cancer Genetics. DNA methylation in prostate cancer Dominic J Smiraglia, PhD Department of Cancer Genetics DNA methylation in prostate cancer Overarching theme Epigenetic regulation allows the genome to be responsive to the environment Sets the tone for

More information

Nanofibrillated cellulose: results of in vitro and in vivo toxicological assays

Nanofibrillated cellulose: results of in vitro and in vivo toxicological assays Nanofibrillated cellulose: results of in vitro and in vivo toxicological assays Final Conference 2.6.212 Hannu Norppa Nanosafety Research Center Hazard assessment approach for NFC Methodology In vitro

More information