1 Supplementary Figure 1. Identification of tumorous sphere-forming CSCs and CAF feeder cells. The LEAP (Laser-Enabled Analysis and Processing) platform with laser manipulation to efficiently purify lung CSCs colonies from the co-cultured CAFs. (I) Imaging of wells, setting the colony purification region for the CLS1/CAF co-culture system with square shapes. (II) Imaging of the entire well after laser-processing. Scale bar, 30mm. The purified areas follow the set laser-shooting pattern. CLS1 colony images of the same view before (III) and after (IV) LEAP processing. (V) Image of a purified CLS1 colony cells after washing the cells with PBS. Scale bar, 5mm.
2 Supplementary Figure 2. The primary culture of lung cancer stem cells and CAFs from NSCLC patients. (a) Tumorous sphere-forming CSCs cultured with CAF feeder cells derived from primary tumor tissues resected from different lung cancer patients (PT207, 013, 270, 450, 220, and 290). The patient demographics, tumor stage, and pathological diagnosis are listed as Supplementary Table 2. Scale bar, 100 m. (b) DNA aneuploidy analysis of CLS1 cells. (c) Flow cytometric analysis of fibroblast surface markers defined the phenotype of CAF feeder cells. The CAFs were positive stained with human fibroblast-specific CD90, HLA-A, -B, and -C (class I), and myofibroblast-specific alpha-sma.
3 Supplementary Figure 3. CAFs co-culture system could be a platform for maintaining lung cancer stemness. (a) Lung CSCs (CLS1) co-cultured with CAFs for 10 days formed similar morphology tumorous spheres in different time point (upper). Scale bar, 50 m. RT Q-PCR analysis of the stem cell markers Nanog and Oct3/4 in CLS1 cells co-cultured with CAFs (CLS1/CAF) in different passages (CLS1/CAF p.3, CLS1/CAF p.6, and CLS1/CAF p.16) (lower), comparing to CAFs and differentiated CLS1 cells.(n=3) (b) The incidence of mouse xenograft tumors from CLS1/CAF co-cultures in different passage (CLS1/CAF p.3, CLS1/CAF p.12, and CLS1/CAF p.16) (N=4-6 mice) in different cell numbers (5 10 6, , , , , and cells) injected subcutaneously into SCID mice. Data represent the mean ±S.D. and tested for significance by Student s t-test *P<0.05. Data are representative of three independent biological experiments each.
4 Supplementary Figure 4. Lung CSCs can maintain stemness ability in vivo. (a) The morphology of tumorous sphere of tumor cells isolated from subcutaneous tumor (SC1-4) and spontaneous lung metastasis (meta-lung) nodules of CLS1/CAF xenograft tumors. Scale bar, 100 m. (b) The incidence of mouse xenograft tumors with sub-cultured CLS1 cells isolated from a CLS1/CAF-derived subcutaneous xenograft (SC-xenograft) (N=4 or 5 mice) and lung metastasis tumor (N=4 or 5 mice). Different numbers of cells (1 10 4, , and cells) were subcutaneously injected into the SCID mice.
5 Supplementary Figure 5. The characterization of lung cancer stem cells under co-culturing with CAFs. (a) The tumorous ultra-low sphere-forming assay showing the morphology of the tumorous spheres of cancer cells/cafs and differentiated cancer cells in different primary lung cancer cell lines (CLS1, CL141, and CL152) seeded in ultra-low adherent plates in MCDB201 medium with EGF (20 ng/ml) and bfgf (20 ng/ml) for 21 days (upper panel). The sphere numbers (N=6) and sphere area (N=10) were quantified (lower panel). Scale bar, 100 m. (b) The ALDH activity of the primary lung cancer cell lines (CLS1, CL141 and CL152) cultured with or without CAFs. The ALDH activity was stained with the ALDEFLUOR dye in the absence (upper) or presence (lower) of DEAB (an ALDH inhibitor) for 30 mins at 37 and analyzed by flow cytometry. (c-d) Side-population analysis of cancer cells in lung cancer cell lines (CLS1-5, A549, H522, H23, Hop62, H322M, and EKVX) and CLS1 cells cultured with or without CAF. Cells were stained with the Hoechst dye (5 g/ml) in the absence (left) or presence (right) of 50 M reserpine for 2 h at 37 and then analyzed by flow cytometry. Data represent the mean ±S.D. and tested for significance by Student s t-test *P<0.05. Data are representative of at least three independent biological experiments each.
6 Supplementary Figure 6. Lung CSCs differentiated into different types of cancer cells in vitro and in vivo. (a) Immunofluorescence staining with different cancer subtype markers, including transcription thyroid factor 1 (TTF-1), cytokeratin-7 (CK7), and cytokeratin-20 (CK20) (for lung adenocarcinoma) or p63 and keratin-5/6 (for squamous cell carcinoma) in different clones of tumor cells (C1, C2, C3, and C4). Scale bar, 20 m. (b) Immunohistochemistry of TTF-1, CK7, CK7, p63, keratin-5/6, and mucin-1 in xenograft tumors (subcutaneous and spontaneous lung metastatic tumors) derived from injection of CLS1 cells co-cultured with CAF feeder cells. A549 cells served as a control. Scale bar, 50 m.
7 Supplementary Figure 7. Different clones of differentiated CLS1 can de-differentiate through re-co-culturing with CAFs. (a) Immunofluorescence staining with different cancer subtype markers, including transcription thyroid factor 1 (TTF-1), cytokeratin-7 (CK7), and cytokeratin-20 (CK20) (for lung adenocarcinoma) or p63 and keratin-5/6 (for squamous cell carcinoma) in different clones of tumor cells (C1, C2, C3, and C4) co-cultured with CAFs (CLS1/CAF). Scale bar, 20 m. (b) RT Q-PCR analysis of the stem cell markers Nanog and Oct3/4 in different clones of tumor cells (C1, C2, C3, and C4) co-cultured with CAFs (CLS1/CAF), comparing to different clones of tumor cells. (N=3) Data represent the mean ±S.D. and tested for significance by Student s t-test *P<0.05. Data are representative of at least three independent biological experiments each. Scale bar, 50 m.
8 Supplementary Figure 8. Full genome Gene expression profiling reveals key signaling pathway in CSCs. (a) Hierarchical clustering of differentially expressed genes in CLS1 cultured with and without CAF feeder cells. Metacore pathway analysis showed up-regulation of genes involved in the EMT, PI3K, IGF1R, TGF-beta, WNT, and Hedgehog signaling pathways in CLS1/CAF co-cultures. (b) Signaling pathway networks that regulate cancer stemness in lung CSCs (Green line means activated, red line as inactivated and pink indicates unspecified). (c) Real-time RT Q-PCR analysis of TGF beta-related pathway molecules (TGFBR1 and SMAD2), a Wnt-related pathway molecule (TCF21), a EGFR-related pathway molecule (EGR1), and LIF-related pathway molecules (LIF and LIFR) in the CLS1/CAF co-culture for 10 days and after serial passages (p3 and p14) of CLS1 cells cultured in the absence of CAFs. Data represent the mean ±S.D. and tested for significance by Student s t-test *P<0.05. Data are representative of three independent biological experiments each.
9 Supplementary Figure 9. Potential paracrine interactions between CAFs and CLS1 cells validated by real-time RT Q-PCR. Candidate genes involved in paracrine cross-talk between CAFs and CSCs (CLS1), including IGF2R, IGFBP1, IGFBP2, IGFBP5, SPARC, decorin, and thromobospondin-1, were validated by Q-PCR. The CLS1 cells (after passage p3, p6, and p14) were derived from CLS1 spheres dissociated to single cells and then sub-cultured without CAFs. The CAFs served as the feeder cell control. Data represent the mean ±S.D. and tested for significance by Student s t-test *P<0.05. Data are representative of three independent biological experiments each.
10 Supplementary Figure10. Reciprocal interaction of genes induced in CAFs by co-culture with lung carcinoma cells. RT Q-PCR analysis of HGF and SDF1 in different patient s CAFs (N=5 patients) cultured with or without A549, EKVX and CLS1 cells. Data represent the mean ±S.D. and tested for significance by Student s t-test *P<0.05. Data are representative of at least three independent biological experiments each.
11 Supplementary Figure 11. The procedures of identifying cancer stem cell through high content analysis. (a) CD90 as the CAF marker; Nanog for the stemness cells; DAPI staining for the nuclei and helpful for identifying the CSC colony by image-based high content analysis. Colony numbers, area, cells per colony, and Nanog-postive cells. Scale bar, 2mm. (b) Image-based high content analysis showing the Nanog (+) colony-forming ability of CLS1 cells increased under the treatment of IGF-II (10 and 100 ng/ml). Data represent the mean ±S.D. and tested for significance by Student s t-test *P<0.05. Data are representative of three independent biological experiments each. Scale bar, 2mm.
12 Supplementary Figure 12. IGF-II induced stemness in different lung cancer cell lines. (a) Flow cytometric analysis with ALDEFLUOR assay of CLS1/CAFs cells treated with IGF-II (100 ng/ml). The fluorescence intensity corresponding to the ALDH activity was measured without DEAB (upper panel) and with DEAB (lower panel). (b) The ALDH activity was examined by flow cytometry in four different lung cancer cell lines (CLS1/CAFs, A549, H1975, and EKVX) treated with IGF-II (100 ng/ml). Data represent the mean ±S.D. and tested for significance by Student s t-test *P<0.05. Data are representative of three independent biological experiments each.
13 Supplementary Figure 13. Blockage of IGF1R signaling by PI3K inhibitor suppresses lung cancer stemness in vitro. (a) The Nanog-positive stem cells per colony were analyzed by image-based high content analysis. The different primary lung cancer cell lines (CLS1, CL141 and CL152) were co-cultured with CAFs treated with or without the PI3K inhibitor (LY under 1, 5 10 M/ml).(N=3) (b) Western blot analysis of Nanog in CLS1 cells treated with IGF-II (10 ng/ml) or IGF-II (10 ng/ml) in combination with PI3K inhibitor (LY294002, 5 M/ml) for 24 h. (c) The tumorigenesis ability of xenograft tumors from CLS1/CAF cells was reduced by treatment with LY The tumors were generated by the injection of 1,000 CLS1/CAF cells. The tumor weight of the CLS1/CAF cells was decreased by the LY (5 M/ml) treatment (N=5 mice each group). Data represent the mean ±S.D. and tested for significance by Student s t-test *P<0.05. Data are representative of three independent biological experiments each.
14 Supplementary Figure 14. Blockage of IGF1R signaling by IGF1R neutralize Ab or inhibitor suppresses stemness in different lung cancer cell lines. (a) The A549, and EKVX cells were co-cultured with CAFs treated with or without IGF1R Ab (1 g/ml); the number of Nanog-positive stem cells per colony was examined. (N=3) Scale bar, 100 m. (b) The Nanog-positive stem cells per colony were analyzed by image-based high content analysis. The different primary lung cancer cell lines (CLS1, CL141 and CL152) were co-cultured with CAFs treated with or without the IGF1R kinase inhibitor (AEW541 under 1, 5 10 M/ml). (N=3) (c) The sphere-forming morphology and ability (Numbers of sphere,n=6; Average area of sphere, N=10) of the lung cancer cell lines, including A549 and EKVX, were significantly reduced by treatment with IGF1R Ab (1 g/ml). Data represent the mean ±S.D. and tested for significance by Student s t-test *P<0.05. Data are representative of at least three independent biological experiments each. Scale bar, 200 m.
15 Supplementary Figure 15. Blockage of IGF1R signaling by IGF1R kinase inhibitors suppresses lung cancer stemness in vivo. The tumorigenesis ability of xenograft tumors from CLS1/CAF cells was reduced by treatment with AEW541. The tumors were generated by the injection of 1,000 CLS1/CAF cells. The tumor weight of the CLS1/CAF cells was decreased by the AEW541 (5 M/ml) treatment (N=5 mice each group).
16 Supplementary Figure 16. Full-scan images of representative Western blots.
17 Supplementary Figure 16. Continued
18 Supplementary Figure 16. Continued
19 Supplementary Figure 16. Continued
20 Supplementary Table 1. Patient demographics, tumor stage, pathological diagnosis Patients Age Sex TNM Stage Histology Primary cultured Cell PT F T1bN0 Stage IA ADC Cancer cell and CAFs PT M T4N Stage IV ADC Cancer cell and CAFs PT M T3N3M1b Stage IV SCLC Cancer cell and CAFs PT M T3N3M1a Stage IV ADC Cancer cell and CAFs PT M T2bN0 Stage IIA ADC CAFs and NFs PT M T3N0 Stage IIB SCC Cancer cell and CAFs PT F T2aN0 Stage IB ADC CAFs and NFs PT M T4N0 Stage IIIB SCC CAFs and NFs PT M T4N3M1b Stage IV ADC Cancer cell and CAFs PT F T2aN0 Stage IIA ADC CAFs and NFs PT M T3N0 Stage IIB SCC CAFs and NFs CLS1 87 M T2bN0 Stage IIIA ASC Cancer cell and CAFs ASC: Adenosquamous carcinoma; ADC: Adenocarcinoma; SCC: Squamous cell carcinoma; SCLC: Small cell lung cancer.
21 Supplementary Table 2. The Metastasis Incidence of CLS1/CAFs, CLS1 and A549 cells Cells Lung metastasis/ mice CLS1/CAFs 12/15 CLS1 2/10 A549 0/3
22 Supplementary Table 3. The IC 50 of CLS1/CAF and CLS1 treated with chemotherapeutic compounds. Drugs IC 50 CLS1/CAF CLS1 Etoposide (VP-16 ) M 1.06 M Docetaxel 54.8 nm 4.2 nm Vinorelbine ditartrate nm 28.5 nm Cisplatin 0.31 M 0.20 M
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