In vitro scratch assay: method for analysis of cell migration in vitro labeled fluorodeoxyglucose (FDG)

Save this PDF as:
 WORD  PNG  TXT  JPG

Size: px
Start display at page:

Download "In vitro scratch assay: method for analysis of cell migration in vitro labeled fluorodeoxyglucose (FDG)"

Transcription

1 In vitro scratch assay: method for analysis of cell migration in vitro labeled fluorodeoxyglucose (FDG) 1 Dr Saeb Aliwaini 13/11/2015

2 Migration in vivo Primary tumors are responsible for only about 10% of deaths from cancer. The remaining approximately 90% of patients are struck down by cancerous growths that are discovered at sites far removed from the locations in their bodies where their primary tumors first arose. (metastases ) Using body fluids Breast cancer: often spawn metastatic colonies promiscuously in many tissues throughout the body, including the brain, liver, bones, and lungs. 2 Dr Saeb Aliwaini 13/11/2015

3 It depends on a series of complex biological steps Carcinomas : 80% of cancers 3 Dr Saeb Aliwaini 13/11/2015

4 Migration in vivo Local invasion seems to depend invariably on the release of secreted proteases, which are required to remodel the extracellular matrix (ECM), thereby generating space for the advance of cancer cells. In order to acquire motility and invasiveness, carcinoma cells must shed many of their epithelial phenotypes, detach from epithelial sheets, and undergo a drastic alteration the epithelial mesenchymal transition (EMT). 4 Dr Saeb Aliwaini 13/11/2015

5 EMT EMT involves a shedding by epithelial cells of their characteristic morphology and gene expression pattern and the assumption of a shape and transcriptional program characteristic of mesenchymal cells. An EMT can also be seen at the edges of carcinomas that are invading adjacent tissues 5 Dr Saeb Aliwaini 13/11/2015

6 6 Dr Saeb Aliwaini 13/11/2015

7 EMT Expression of E-cadherin and cytokeratins hallmarks of epithelial cell protein expression is repressed, while the expression of vimentin, an intermediate filament component of the mesenchymal cell cytoskeleton, is induced. Epithelial cells that have undergone an EMT often begin to make fibronectin, an extracellular matrix protein that is normally secreted only by mesenchymal cells such as fibroblasts. At the same time, expression of a typical fibroblastic marker N-cadherin is often acquired in place of E-cadherin 7 Dr Saeb Aliwaini 13/11/2015

8 EMT The transmembrane E-cadherin molecule plays the dominant role in influencing epithelial versus mesenchymal cell phenotypes adherens junctions For example, an analysis of 26 human breast cancer cell lines indicated that 16 had mutations in E-cad. The N-cadherin molecules expressed on the surface of a carcinoma cell that has undergone an EMT increase the affinity of this cancer cell for the stromal cells that normally display N-cadherin. 8 Dr Saeb Aliwaini 13/11/2015

9 9 Dr Saeb Aliwaini 13/11/2015

10 In vitro scratch assay The in vitro scratch assay is a straightforward and economical method to study cell migration in vitro. This method is based on the observation that, upon creation of a new artificial gap, so called scratch, on a confluent cell monolayer, the cells on the edge of the newly created gap will move toward the opening to close the scratch until new cell cell contacts are established again. 10 Dr Saeb Aliwaini 13/11/2015

11 In vitro scratch assay It mimics to some extent migration of cells in vivo - Removal of part of the endothelium in the blood vessels will induce migration of endothelial cells (ECs) into the denuded area to close the wound. Can be done for every cell type With specific ECM It is also compatible with microscopy including live cell imaging To study the effect of gene expression, drugs 11 Dr Saeb Aliwaini 13/11/2015

12 Transwell Migration Assay The transwell migration assay is a commonly used test to study the migratory response of endothelial cells to angiogenic inducers or inhibitors. This assay is also known as the Boyden or modified Boyden chamber assay. Cells are placed on the upper layer of a permeable membrane and a solution containing the test agent is placed below the cell permeable membrane. Following an incubation period (3 18 hours), the cells that have migrated through the membrane are stained and counted. The membrane is usually coated with some extracellular matrix 12 Dr Saeb Aliwaini 13/11/2015

13 13 Dr Saeb Aliwaini 13/11/2015

14 Revision cell cycle and apoptosis assays When do you do cell cycle analysis? What will you conclude? What is next? 14 Dr Saeb Aliwaini 13/11/2015

15 Cell Cycle Analysis DNA content analysis - Propidium Iodide S Phase G0/G1 G2/M Or doublets of G0/G1 cells? DNA Content 13/11/2015 Dr Saeb Aliwaini 15

16 Apoptosis Bcl-2 family members (intracellular staining) Fix and permeabilize Add Antibody Analyse by Flow Cytometry 13/11/2015 Dr Saeb Aliwaini 16

17 Apoptosis Bcl-2 family members Bcl-2 13/11/2015 Dr Saeb Aliwaini 17

18 Apoptosis Propidium Iodide (fixed cells) DNA degradation 13/11/2015 Dr Saeb Aliwaini 18

19 Apoptosis Annexin V-fluorochrome plus Propidium Iodide (non-fixed cells) 13/11/2015 Dr Saeb Aliwaini 19

20 Apoptosis Annexin V plus propidium Iodide 13/11/2015 Dr Saeb Aliwaini 20

21 Apoptosis Annexin V plus propidium Iodide 13/11/2015 Dr Saeb Aliwaini 21

The Hallmarks of Cancer

The Hallmarks of Cancer The Hallmarks of Cancer Theresa L. Hodin, Ph.D. Clinical Research Services Theresa.Hodin@RoswellPark.org Hippocrates Cancer surgery, circa 1689 Cancer Surgery Today 1971: Nixon declares War on Cancer

More information

An epithelial-to-mesenchymal transition-inducing potential of. granulocyte macrophage colony-stimulating factor in colon. cancer

An epithelial-to-mesenchymal transition-inducing potential of. granulocyte macrophage colony-stimulating factor in colon. cancer An epithelial-to-mesenchymal transition-inducing potential of granulocyte macrophage colony-stimulating factor in colon cancer Yaqiong Chen, Zhi Zhao, Yu Chen, Zhonglin Lv, Xin Ding, Renxi Wang, He Xiao,

More information

Cell Migration and Invasion Assays INCUCYTE LIVE-CELL ANALYSIS SYSTEM. Real-time automated measurements of cell motility inside your incubator

Cell Migration and Invasion Assays INCUCYTE LIVE-CELL ANALYSIS SYSTEM. Real-time automated measurements of cell motility inside your incubator Cell Migration and Invasion Assays INCUCYTE LIVE-CELL ANALYSIS SYSTEM Real-time automated measurements of cell motility inside your incubator See the whole story Real-time cell motility visualization and

More information

Advanced Cell Biology. Lecture 36

Advanced Cell Biology. Lecture 36 Advanced Cell Biology. Lecture 36 Alexey Shipunov Minot State University May 3, 2013 Shipunov (MSU) Advanced Cell Biology. Lecture 36 May 3, 2013 1 / 43 Outline Questions and answers Cellular communities

More information

mir-509-5p and mir-1243 increase the sensitivity to gemcitabine by inhibiting

mir-509-5p and mir-1243 increase the sensitivity to gemcitabine by inhibiting mir-509-5p and mir-1243 increase the sensitivity to gemcitabine by inhibiting epithelial-mesenchymal transition in pancreatic cancer Hidekazu Hiramoto, M.D. 1,3, Tomoki Muramatsu, Ph.D. 1, Daisuke Ichikawa,

More information

The Annexin V Apoptosis Assay

The Annexin V Apoptosis Assay The Annexin V Apoptosis Assay Development of the Annexin V Apoptosis Assay: 1990 Andree at al. found that a protein, Vascular Anticoagulant α, bound to phospholipid bilayers in a calcium dependent manner.

More information

1.The metastatic cascade. 2.Pathologic features of metastasis. 3.Therapeutic ramifications

1.The metastatic cascade. 2.Pathologic features of metastasis. 3.Therapeutic ramifications Metastasis 1.The metastatic cascade 2.Pathologic features of metastasis 3.Therapeutic ramifications Sir James Paget (1814-1899) British Surgeon/ Pathologist Paget s disease of bone Paget s disease of the

More information

Journal Club Semmler Lorenz

Journal Club Semmler Lorenz Beer et al. 2015 - Analysis of the Secretome of Apoptotic Peripheral Blood Mononuclear Cells: Impact of Released Proteins and Exosomes for Tissue Regeneration Journal Club 13.11.2017 1 Introduction to

More information

Modelling Cancer Cell Migration and Invasion Using Novel Technology for Three Dimensional Cell Culture. Introduction

Modelling Cancer Cell Migration and Invasion Using Novel Technology for Three Dimensional Cell Culture. Introduction Modelling Cancer Cell Migration and Invasion Using Novel Technology for Three Dimensional Cell Culture Application Note 3 Introduction Cancer is characterised by unregulated and abnormal cell growth in

More information

Impact of NM23-M1 "knock-out" on metastatic potential of hepatocellular carcinoma: a transgenic approach

Impact of NM23-M1 knock-out on metastatic potential of hepatocellular carcinoma: a transgenic approach Impact of NM23-M1 "knock-out" on metastatic potential of hepatocellular carcinoma: a transgenic approach NM23-M1 "knock- out" mice (without NDPK A) Experimental models of hepatocarcinogenesis Chemical

More information

MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question.

MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. Exam Name MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1) All of the following are synthesized along various sites of the endoplasmic reticulum

More information

I TESSUTI: Dott.ssa Liliana Belgioia Università degli Studi di Genova

I TESSUTI: Dott.ssa Liliana Belgioia Università degli Studi di Genova I TESSUTI: 1. Repair, Radiosensitivity, Recruitment, Repopulation, Reoxygenation 2. Acute and chronic hypoxia 3. Tissue microenvironment and tissue organization Dott.ssa Liliana Belgioia Università degli

More information

Tissue renewal and Repair. Nisamanee Charoenchon, PhD Department of Pathobiology, Faculty of Science

Tissue renewal and Repair. Nisamanee Charoenchon, PhD   Department of Pathobiology, Faculty of Science Tissue renewal and Repair Nisamanee Charoenchon, PhD Email: nisamanee.cha@mahidol.ac.th Department of Pathobiology, Faculty of Science Topic Objectives 1. Describe processes of tissue repair, regeneration

More information

Award Number: W81XWH TITLE: Characterizing an EMT Signature in Breast Cancer. PRINCIPAL INVESTIGATOR: Melanie C.

Award Number: W81XWH TITLE: Characterizing an EMT Signature in Breast Cancer. PRINCIPAL INVESTIGATOR: Melanie C. AD Award Number: W81XWH-08-1-0306 TITLE: Characterizing an EMT Signature in Breast Cancer PRINCIPAL INVESTIGATOR: Melanie C. Bocanegra CONTRACTING ORGANIZATION: Leland Stanford Junior University Stanford,

More information

Jianhua Qin. Dalian Institute of Chemical Physics Chinese Academy of Sciences

Jianhua Qin. Dalian Institute of Chemical Physics Chinese Academy of Sciences Jianhua Qin Dalian Institute of Chemical Physics Chinese Academy of Sciences Outline What and why microfluidics? Cancer microenvironment on chip Multicellular organism on chip Summary and futures Microfluidics

More information

Linda Lowe Krentz Bioscience in the 21 st Century October 22, 2010

Linda Lowe Krentz Bioscience in the 21 st Century October 22, 2010 Cell Biomechanics Linda Lowe Krentz Bioscience in the 21 st Century October 22, 2010 Outline for today Tubes Vessel mechanics and disease Models Lung mechanics Technology integration Atherosclerosis is

More information

1. The metastatic cascade. 3. Pathologic features of metastasis. 4. Therapeutic ramifications. Which malignant cells will metastasize?

1. The metastatic cascade. 3. Pathologic features of metastasis. 4. Therapeutic ramifications. Which malignant cells will metastasize? 1. The metastatic cascade 3. Pathologic features of metastasis 4. Therapeutic ramifications Sir James Paget (1814-1899) British Surgeon/ Pathologist Paget s disease of Paget s disease of the nipple (intraductal

More information

Regulation of the Cell Cycle CHAPTER 12

Regulation of the Cell Cycle CHAPTER 12 Regulation of the Cell Cycle CHAPTER 12 Regulation of the Cell Cycle Timing and rates of cell division in different parts of an animal or plant are crucial for normal growth, development, and maintenance.

More information

Test Bank for Robbins and Cotran Pathologic Basis of Disease 9th Edition by Kumar

Test Bank for Robbins and Cotran Pathologic Basis of Disease 9th Edition by Kumar Link full download:https://getbooksolutions.com/download/test-bank-for-robbinsand-cotran-pathologic-basis-of-disease-9th-edition-by-kumar Test Bank for Robbins and Cotran Pathologic Basis of Disease 9th

More information

Chapter 2 (pages 22 33): Cells and Tissues of the Immune System. Prepared by Kristen Dazy, MD, Scripps Clinic Medical Group

Chapter 2 (pages 22 33): Cells and Tissues of the Immune System. Prepared by Kristen Dazy, MD, Scripps Clinic Medical Group Allergy and Immunology Review Corner: Cellular and Molecular Immunology, 8th Edition By Abul K. Abbas, MBBS; Andrew H. H. Lichtman, MD, PhD; and Shiv Pillai, MBBS, PhD. Chapter 2 (pages 22 33): Cells and

More information

Cell Cell Communication

Cell Cell Communication IBS 8102 Cell, Molecular, and Developmental Biology Cell Cell Communication January 29, 2008 Communicate What? Why do cells communicate? To govern or modify each other for the benefit of the organism differentiate

More information

(A) Dose response curves of HMLE_shGFP (blue circle), HMLE_shEcad (red square),

(A) Dose response curves of HMLE_shGFP (blue circle), HMLE_shEcad (red square), Supplementary Figures and Tables Figure S1. Validation of EMT-selective small molecules (A) Dose response curves of HMLE_shGFP (blue circle), HMLE_shEcad (red square), and HMLE_Twist (black diamond) cells

More information

TITLE: Hyaluronan-CD44 Interactions Decrease the Metastatic Potential of Breast Cancer Cells

TITLE: Hyaluronan-CD44 Interactions Decrease the Metastatic Potential of Breast Cancer Cells Award Number: W81XWH-06-1-0455 TITLE: Hyaluronan-CD44 Interactions Decrease the Metastatic Potential of Breast Cancer Cells PRINCIPAL INVESTIGATOR: Jose I. Lopez CONTRACTING ORGANIZATION: The University

More information

The Avatar System TM Yields Biologically Relevant Results

The Avatar System TM Yields Biologically Relevant Results Application Note The Avatar System TM Yields Biologically Relevant Results Liquid biopsies stand to revolutionize the cancer field, enabling early detection and noninvasive monitoring of tumors. In the

More information

The Angiopoietin Axis in Cancer

The Angiopoietin Axis in Cancer Ang2 Ang1 The Angiopoietin Axis in Cancer Tie2 An Overview: The Angiopoietin Axis Plays an Essential Role in the Regulation of Tumor Angiogenesis Growth of a tumor beyond a limiting size is dependent upon

More information

DNA Microarray Analysis of the Epithelial Mesenchymal Transition of Mesothelial Cells in a Rat Model of Peritoneal Dialysis

DNA Microarray Analysis of the Epithelial Mesenchymal Transition of Mesothelial Cells in a Rat Model of Peritoneal Dialysis Advances in Peritoneal Dialysis, Vol. 27, 2011 Toshimi Imai, Ichiro Hirahara, Yoshiyuki Morishita, Akira Onishi, Makoto Inoue, Shigeaki Muto, Eiji Kusano DNA Microarray Analysis of the Epithelial Mesenchymal

More information

Nimbolide inhibits pancreatic cancer growth and metastasis through ROS-mediated

Nimbolide inhibits pancreatic cancer growth and metastasis through ROS-mediated Nimbolide inhibits pancreatic cancer growth and metastasis through ROS-mediated apoptosis and inhibition of epithelial-to-mesenchymal transition Ramadevi Subramani a, Ph.D., Elizabeth Gonzalez b, MS.,

More information

Cochlear anatomy, function and pathology III. Professor Dave Furness Keele University

Cochlear anatomy, function and pathology III. Professor Dave Furness Keele University Cochlear anatomy, function and pathology III Professor Dave Furness Keele University d.n.furness@keele.ac.uk Aims and objectives of this lecture Focus (3) on the cochlear lateral wall and Reissner s membrane:

More information

The Golgi Apparatus: Shipping and Receiving Center. The Golgi apparatus. Functions of the Golgi apparatus. Lysosomes: Digestive Compartments

The Golgi Apparatus: Shipping and Receiving Center. The Golgi apparatus. Functions of the Golgi apparatus. Lysosomes: Digestive Compartments The Golgi Apparatus: Shipping and Receiving Center The Golgi apparatus Receives (on the cis-side) many of the transport vesicles produced in the rough ER Consists of flattened membranous sacs called cisternae

More information

Flores-Pérez A et al Suppression of cell migration is promoted by mir-944 through targeting of SIAH1 and PTP4A1 in breast cancer cells

Flores-Pérez A et al Suppression of cell migration is promoted by mir-944 through targeting of SIAH1 and PTP4A1 in breast cancer cells Author s response to reviews Title: Suppression of cell migration is promoted by mir-944 through targeting of SIAH1 and PTP4A1 in breast cancer cells Authors: Cesar Lopez-Camarillo (genomicas@yahoo.com.mx)

More information

Production of Exosomes in a Hollow Fiber Bioreactor

Production of Exosomes in a Hollow Fiber Bioreactor Production of Exosomes in a Hollow Fiber Bioreactor John J S Cadwell, President and CEO, FiberCell Systems Inc INTRODUCTION Exosomes are small lipid membrane vesicles (80-120 nm) of endocytic origin generated

More information

Epithelial to Mesenchymal Transition in Renal Fibrogenesis: Pathologic Significance, Molecular Mechanism, and Therapeutic Intervention

Epithelial to Mesenchymal Transition in Renal Fibrogenesis: Pathologic Significance, Molecular Mechanism, and Therapeutic Intervention REVIEW J Am Soc Nephrol 15: 1 12, 2004 Epithelial to Mesenchymal Transition in Renal Fibrogenesis: Pathologic Significance, Molecular Mechanism, and Therapeutic Intervention YOUHUA LIU Division of Cellular

More information

Kelly J.Gordon 1, Mei Dong 2, Elizabeth M.Chislock 1, Timothy A.Fields 3 and Gerard C.Blobe 1,2,

Kelly J.Gordon 1, Mei Dong 2, Elizabeth M.Chislock 1, Timothy A.Fields 3 and Gerard C.Blobe 1,2, Carcinogenesis vol.29 no.2 pp.252 262, 2008 doi:10.1093/carcin/bgm249 Advance Access publication November 13, 2007 Loss of type III transforming growth factor b receptor expression increases motility and

More information

T-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes:

T-cell activation T cells migrate to secondary lymphoid tissues where they interact with antigen, antigen-presenting cells, and other lymphocytes: Interactions between innate immunity & adaptive immunity What happens to T cells after they leave the thymus? Naïve T cells exit the thymus and enter the bloodstream. If they remain in the bloodstream,

More information

The Role of Metastasis Suppressor CD82 in the Deactivation of the c-met Signaling Pathway in Prostate Cancer

The Role of Metastasis Suppressor CD82 in the Deactivation of the c-met Signaling Pathway in Prostate Cancer Grand Valley State University ScholarWorks@GVSU Masters Theses Graduate Research and Creative Practice 4-27-2017 The Role of Metastasis Suppressor CD82 in the Deactivation of the c-met Signaling Pathway

More information

Yuan et al. Breast Cancer Research (2015) 17:69 DOI /s y

Yuan et al. Breast Cancer Research (2015) 17:69 DOI /s y Yuan et al. Breast Cancer Research (2015) 17:69 DOI 10.1186/s13058-015-0579-y RESEARCH ARTICLE Open Access Acquisition of epithelial-mesenchymal transition phenotype in the tamoxifen-resistant breast cancer

More information

IncuCyte Scratch Wound 96-Well Real-Time Cell Migration and Invasion Assays

IncuCyte Scratch Wound 96-Well Real-Time Cell Migration and Invasion Assays IncuCyte Scratch Wound 96-Well Real-Time Cell Migration and Invasion Assays Meagan Roddy, Thom Nelson, Daniel M. Appledorn, and Vince Groppi Essen BioScience Ann Arbor, Michigan Introduction Cell migration

More information

Introduction. Table 1. Metastatic Tumor Formation

Introduction. Table 1. Metastatic Tumor Formation CellPlayer 96-Well Kinetic Cell Migration and Invasion Assays Meagan Roddy, Thom Nelson, Daniel M. Appledorn, and Vince Groppi Essen BioScience Ann Arbor, Michigan Introduction Cell migration is a multistep

More information

Generation of post-germinal centre myeloma plasma B cell.

Generation of post-germinal centre myeloma plasma B cell. Generation of post-germinal centre myeloma. DNA DAMAGE CXCR4 Homing to Lytic lesion activation CD38 CD138 CD56 Phenotypic markers Naive Secondary lymphoid organ Multiple myeloma is a malignancy of s caused

More information

Src-INACTIVE / Src-INACTIVE

Src-INACTIVE / Src-INACTIVE Biology 169 -- Exam 1 February 2003 Answer each question, noting carefully the instructions for each. Repeat- Read the instructions for each question before answering!!! Be as specific as possible in each

More information

Corning BioCoat Matrigel Invasion Chamber

Corning BioCoat Matrigel Invasion Chamber Corning BioCoat Matrigel Invasion Chamber Catalog No. 354480, 354481 Guidelines for Use Discovery Labware, Inc., Two Oak Park, Bedford, MA 01730, Tel: 1.978.442.2200 (U.S.) CLSTechServ@Corning.com www.corning.com/lifesciences

More information

Trevigen s Extracellular Matrix Proteins for Triculture of Breast Cancer Spheroids, Endothelial Tubules, and Human Mesenchymal Stem Cells

Trevigen s Extracellular Matrix Proteins for Triculture of Breast Cancer Spheroids, Endothelial Tubules, and Human Mesenchymal Stem Cells Trevigen s Extracellular Matrix Proteins for Triculture of Breast Cancer Spheroids, Endothelial Tubules, and Human Mesenchymal Stem Cells Gabriel Benton, Ph.D. and Jay George, Ph.D., Trevigen, Inc. Gaithersburg,

More information

Supplementary Figure 1. HOPX is hypermethylated in NPC. (a) Methylation levels of HOPX in Normal (n = 24) and NPC (n = 24) tissues from the

Supplementary Figure 1. HOPX is hypermethylated in NPC. (a) Methylation levels of HOPX in Normal (n = 24) and NPC (n = 24) tissues from the Supplementary Figure 1. HOPX is hypermethylated in NPC. (a) Methylation levels of HOPX in Normal (n = 24) and NPC (n = 24) tissues from the genome-wide methylation microarray data. Mean ± s.d.; Student

More information

Fang et al. NMuMG. PyVmT unstained Anti-CCR2-PE MDA-MB MCF MCF10A

Fang et al. NMuMG. PyVmT unstained Anti-CCR2-PE MDA-MB MCF MCF10A A NMuMG PyVmT 16.5+.5 47.+7.2 Fang et al. unstained Anti-CCR2-PE 4T1 Control 37.6+6.3 56.1+.65 MCF1A 16.1+3. MCF-7 3.1+5.4 MDA-M-231 42.1+5.5 unstained Secondary antibody only Anti-CCR2 SUPPLEMENTAL FIGURE

More information

Urogenital Development

Urogenital Development 2-5-03 Urogenital Development Greg Dressler Assoc. Professor Dept. of Pathology x46490 Dressler@umich.edu The Origin of the Kidney In the vertebrate embryo, the first stage of kidney development occurs

More information

Part I. An Introduction to Cancer

Part I. An Introduction to Cancer Part I An Introduction to Cancer 2 Chapter 1 Cancer: Descriptive Overview Cancer is a disease in which cells propagate uncontrollably. These cells can come from many different parts of the body and the

More information

04_polarity. The formation of synaptic vesicles

04_polarity. The formation of synaptic vesicles Brefeldin prevents assembly of the coats required for budding Nocodazole disrupts microtubules Constitutive: coatomer-coated Selected: clathrin-coated The formation of synaptic vesicles Nerve cells (and

More information

Chapt 15: Molecular Genetics of Cell Cycle and Cancer

Chapt 15: Molecular Genetics of Cell Cycle and Cancer Chapt 15: Molecular Genetics of Cell Cycle and Cancer Student Learning Outcomes: Describe the cell cycle: steps taken by a cell to duplicate itself = cell division; Interphase (G1, S and G2), Mitosis.

More information

Tissues. Definition. A group of similar cells and their intercellular substances specialized to perform a specific function.

Tissues. Definition. A group of similar cells and their intercellular substances specialized to perform a specific function. Chapter 4 - Tissues Tissues Definition A group of similar cells and their intercellular substances specialized to perform a specific function. Tissues Epithelial covers exposed surfaces, lines internal

More information

Computational Systems Biology: Biology X

Computational Systems Biology: Biology X Bud Mishra Room 1002, 715 Broadway, Courant Institute, NYU, New York, USA L#5:(October-18-2010) Cancer and Signals Outline 1 2 Outline 1 2 Cancer is a disease of malfunctioning cells. Cell Lineage: Adult

More information

- 1 - Cell types Monocytes THP-1 cells Macrophages. LPS Treatment time (Hour) IL-6 level (pg/ml)

- 1 - Cell types Monocytes THP-1 cells Macrophages. LPS Treatment time (Hour) IL-6 level (pg/ml) Supplementary Table ST1: The dynamic effect of LPS on IL-6 production in monocytes and THP-1 cells after GdA treatment. Monocytes, THP-1 cells and macrophages (5x10 5 ) were incubated with 10 μg/ml of

More information

C ELL CANCER CELL ASSAYS. Cell Adhesion. Cell Invasion/ Cell Migration. Endothelial Cell Invasion. Tube Formation. 3-D Culture.

C ELL CANCER CELL ASSAYS. Cell Adhesion. Cell Invasion/ Cell Migration. Endothelial Cell Invasion. Tube Formation. 3-D Culture. C ELL I N VA S I O N P RO D U C T S Cell Adhesion Cell Invasion/ Cell Migration Endothelial Cell Invasion Tube Formation Angiogenesis CANCER CELL ASSAYS 3-D Culture CA N C ER CELL AS S AYS The behavior

More information

A. Major parts 1. Nucleus 2. Cytoplasm a. Contain organelles (see below) 3. Plasma membrane (To be discussed in Cellular Transport Lecture)

A. Major parts 1. Nucleus 2. Cytoplasm a. Contain organelles (see below) 3. Plasma membrane (To be discussed in Cellular Transport Lecture) Lecture 5: Cellular Biology I. Cell Theory Concepts: 1. Cells are the functional and structural units of living organisms 2. The activity of an organism is dependent on both the individual and collective

More information

TigerPrints. Clemson University. Erin Vegter Clemson University,

TigerPrints. Clemson University. Erin Vegter Clemson University, Clemson University TigerPrints All Theses Theses 12-2013 THE EFFECT OF CELL CULTURE ENVIRONMENT ON EXPRESSION OF CD44+, KI67, AND FOXO3A AND ITS IMPLICATIONS ON THE ROLE OF FOXO3A IN TRIPLE NEGATIVE BREAST

More information

Microanatomy-Cytology (cells)

Microanatomy-Cytology (cells) Microanatomy-Cytology (cells) Levels of Organization least complex most complex Chemical level>cellular level>tissue level>organ level>organ system level>organism level Cytology Cytology-the study of the

More information

Overview of methodology, tools and reagents for evaluating cell proliferation and invasion using multicellular tumor spheroids.

Overview of methodology, tools and reagents for evaluating cell proliferation and invasion using multicellular tumor spheroids. The Next Step in the Evolution of 3D Culture: Utilizing Extracellular Matrix to Enhance Multicellular Tumor Spheroid Models for Proliferation and Invasion Overview of methodology, tools and reagents for

More information

BL-8040: BEST-IN-CLASS CXCR4 ANTAGONIST FOR TREATMENT OF ONCOLOGICAL MALIGNANCIES. Overview and Mechanism of Action Dr.

BL-8040: BEST-IN-CLASS CXCR4 ANTAGONIST FOR TREATMENT OF ONCOLOGICAL MALIGNANCIES. Overview and Mechanism of Action Dr. BL-8040: BEST-IN-CLASS CXCR4 ANTAGONIST FOR TREATMENT OF ONCOLOGICAL MALIGNANCIES Overview and Mechanism of Action Dr. Leah Klapper, CSO 88 BL-8040: Novel CXCR4 Antagonist For Hematological Cancers Indications:

More information

Next-Generation Immunohistochemistry: Multiplex tissue imaging with mass cytometry

Next-Generation Immunohistochemistry: Multiplex tissue imaging with mass cytometry Nat Met, April 2014 Nat Med, April 2014 Next-Generation Immunohistochemistry: Multiplex tissue imaging with mass cytometry Journal Club Timo Böge Overview Introduction Conventional Immunohistochemistry

More information

يراهظلا( يئلاطلا جيسنلا

يراهظلا( يئلاطلا جيسنلا Epithelium النسيج الطالئي )الظهاري( Features of Epithelium Epithelium occurs in the body as a sheet of cells that covers a body surface, lines a cavity, or forms a gland. Coverings, linings, glands. Derived

More information

Epithelial mesenchymal plasticity in carcinoma metastasis

Epithelial mesenchymal plasticity in carcinoma metastasis REVIEW Epithelial mesenchymal plasticity in carcinoma metastasis Jeff H. Tsai 1 and Jing Yang 1,2,3 1 Department of Pharmacology, 2 Department of Pediatrics, School of Medicine, University of California

More information

The pseudogene TUSC2P promotes TUSC2 function by binding multiple micrornas

The pseudogene TUSC2P promotes TUSC2 function by binding multiple micrornas Received 9 Aug 3 Accepted Nov 3 Published 7 Jan DOI:.38/ncomms39 The pseudogene promotes TUSC function by binding multiple micrornas OPEN Zina Jeyapalan Rutnam,, *, William W. Du,, *, Weining Yang, Xiangling

More information

HLA and antigen presentation. Department of Immunology Charles University, 2nd Medical School University Hospital Motol

HLA and antigen presentation. Department of Immunology Charles University, 2nd Medical School University Hospital Motol HLA and antigen presentation Department of Immunology Charles University, 2nd Medical School University Hospital Motol MHC in adaptive immunity Characteristics Specificity Innate For structures shared

More information

The Pennsylvania State University. The Graduate School of. Integrative Biosciences

The Pennsylvania State University. The Graduate School of. Integrative Biosciences The Pennsylvania State University The Graduate School of Integrative Biosciences THE REGULATION OF MESENCHYMAL AND CANCER STEM CELL PHENOTYPES IN HEPATOCELLULAR CARCINOMA A Dissertation in Molecular Medicine

More information

Uncovering the mechanisms of wound healing and fibrosis

Uncovering the mechanisms of wound healing and fibrosis Any Questions??? Ask now or contact support support@sabiosciences.com 1-888-503-3187 International customers: SABio@Qiagen.com Uncovering the mechanisms of wound healing and fibrosis Webinar related questions:

More information

ACTIVATION OF T LYMPHOCYTES AND CELL MEDIATED IMMUNITY

ACTIVATION OF T LYMPHOCYTES AND CELL MEDIATED IMMUNITY ACTIVATION OF T LYMPHOCYTES AND CELL MEDIATED IMMUNITY The recognition of specific antigen by naïve T cell induces its own activation and effector phases. T helper cells recognize peptide antigens through

More information

Lecture Series 4 Cellular Membranes

Lecture Series 4 Cellular Membranes Lecture Series 4 Cellular Membranes Reading Assignments Read Chapter 11 Membrane Structure Review Chapter 21 pages 709-717 717 (Animal( Cell Adhesion) Review Chapter 12 Membrane Transport Review Chapter

More information

Bihong Zhao, M.D, Ph.D Department of Pathology

Bihong Zhao, M.D, Ph.D Department of Pathology Bihong Zhao, M.D, Ph.D Department of Pathology 04-28-2009 Is tumor self or non-self? How are tumor antigens generated? What are they? How does immune system respond? Introduction Tumor Antigens/Categories

More information

Cellometer Image Cytometry for Cell Cycle Analysis

Cellometer Image Cytometry for Cell Cycle Analysis Cellometer Cytometry for Cell Cycle Analysis Importance of Cell Cycle Research Oncology: Since cancer cells often undergo abnormal cell division and proliferation, it is important to understand the cell

More information

Angiogenesis in Human Development. Vascular Development

Angiogenesis in Human Development. Vascular Development Angiogenesis in Human Development Jan Kitajewski ICRC 217B, ph 851-4688, email: jkk9 BACKGROUND READING: Vascular Development Signaling Vascular Morphogenesis and Maintenance Douglas Hanahan. Science 277:

More information

CytoSelect 24- Well Cell Invasion Assay (Basement Membrane, Fluorometric Format)

CytoSelect 24- Well Cell Invasion Assay (Basement Membrane, Fluorometric Format) Product Manual CytoSelect 24- Well Cell Invasion Assay (Basement Membrane, Fluorometric Format) Catalog Number CBA- 111 12 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction

More information

Four Types of Vertebrate Tissue

Four Types of Vertebrate Tissue BIO 121 Molecular Cell Biology Lecture Section IV A. Cells in the Context of Tissue, Organ and Organismal Architecture B. Wound Healing Four Types of Vertebrate Tissue 1.Epithelium 2.Connective Tissue

More information

Variable Response to Chemotherapeutics by a Subpopulation of MCF-7 Breast Cancer Cells

Variable Response to Chemotherapeutics by a Subpopulation of MCF-7 Breast Cancer Cells Variable Response to Chemotherapeutics by a Subpopulation of MCF-7 Breast Cancer Cells JULIE MARIE DORLAND BIOMEDICAL ENGINEERING AND PLAN II HONORS MAY 17, 2016 ENGINEERING HONORS UNDERGRADUATE THESIS

More information

Partial epithelial-mesenchymal transition in keloid scars: regulation of keloid keratinocyte gene expression by transforming growth factor-β1

Partial epithelial-mesenchymal transition in keloid scars: regulation of keloid keratinocyte gene expression by transforming growth factor-β1 Hahn et al. Burns & Trauma (2016) 4:30 DOI 10.1186/s41038-016-0055-7 RESEARCH ARTICLE Partial epithelial-mesenchymal transition in keloid scars: regulation of keloid keratinocyte gene expression by transforming

More information

Cell Signaling Regulatory Mechanisms Controlling Epithelial-Mesenchymal Transition in Carcinoma

Cell Signaling Regulatory Mechanisms Controlling Epithelial-Mesenchymal Transition in Carcinoma University of Pennsylvania ScholarlyCommons Publicly Accessible Penn Dissertations 1-1-2015 Cell Signaling Regulatory Mechanisms Controlling Epithelial-Mesenchymal Transition in Carcinoma Janine Marie

More information

I. Fluid Mosaic Model A. Biological membranes are lipid bilayers with associated proteins

I. Fluid Mosaic Model A. Biological membranes are lipid bilayers with associated proteins Lecture 6: Membranes and Cell Transport Biological Membranes I. Fluid Mosaic Model A. Biological membranes are lipid bilayers with associated proteins 1. Characteristics a. Phospholipids form bilayers

More information

DAX1, testes development role 7, 8 DFFRY, spermatogenesis role 49 DMRT genes, male sex differentiation role 15

DAX1, testes development role 7, 8 DFFRY, spermatogenesis role 49 DMRT genes, male sex differentiation role 15 Subject Index N-Acetylcysteine, sperm quality effects 71 Ambiguous genitalia, origins 1, 2 Anti-Müllerian hormone function 13 receptors 13 Sertoli cell secretion 10, 38 Apoptosis assays in testes 73, 74

More information

The CXCL5/CXCR2 axis contributes to the epithelial-mesenchymal transition of nasopharyngeal carcinoma cells by activating ERK/GSK-3β/snail signalling

The CXCL5/CXCR2 axis contributes to the epithelial-mesenchymal transition of nasopharyngeal carcinoma cells by activating ERK/GSK-3β/snail signalling Qiu et al. Journal of Experimental & Clinical Cancer Research (2018) 37:85 https://doi.org/10.1186/s13046-018-0722-6 RESEARCH Open Access The CXCL5/CXCR2 axis contributes to the epithelial-mesenchymal

More information

Basic tumor nomenclature

Basic tumor nomenclature Jonas Nilsson jonas.a.nilsson@surgery.gu.se Sahlgrenska Cancer Center Bilder gjorda av Per Holmfeldt och Jonas Nilsson Benign tumor Basic tumor nomenclature Malignant tumor = cancer Metastasis Carcinoma:

More information

Optimizing Intracellular Flow Cytometry:

Optimizing Intracellular Flow Cytometry: Optimizing Intracellular Flow Cytometry: Simultaneous Detection of Cytokines and Transcription Factors Presented by Jurg Rohrer, PhD, BD Biosciences 23-10780-00 Outline Introduction Cytokines Transcription

More information

Cancer genetics

Cancer genetics Cancer genetics General information about tumorogenesis. Cancer induced by viruses. The role of somatic mutations in cancer production. Oncogenes and Tumor Suppressor Genes (TSG). Hereditary cancer. 1

More information

Optimizing Intracellular Flow Cytometry

Optimizing Intracellular Flow Cytometry Optimizing Intracellular Flow Cytometry Detection of Cytokines, Transcription Factors, and Phosphoprotein by Flow Cytometry Presented by Erika O Donnell, PhD, BD Biosciences 23-14876-00 Outline Basic principles

More information

Cancer Research Techniques

Cancer Research Techniques Cancer Research Techniques Identification of Anti-Invasive but Noncytotoxic Chemotherapeutic Agents Using the Tetrazolium Dye MTT to Quantitate Viable Cells in Matrigel BioTechniques 24:1038-1043 (June

More information

Supplementary Figure 1. IL-12 serum levels and frequency of subsets in FL patients. (A) IL-12

Supplementary Figure 1. IL-12 serum levels and frequency of subsets in FL patients. (A) IL-12 1 Supplementary Data Figure legends Supplementary Figure 1. IL-12 serum levels and frequency of subsets in FL patients. (A) IL-12 serum levels measured by multiplex ELISA (Luminex) in FL patients before

More information

Sonic Hedgehog (Shh) Signaling Promotes Tumorigenicity and Stemness via Activation of Epithelial-to-Mesenchymal Transition (EMT) in Bladder Cancer

Sonic Hedgehog (Shh) Signaling Promotes Tumorigenicity and Stemness via Activation of Epithelial-to-Mesenchymal Transition (EMT) in Bladder Cancer MOLECULAR CARCINOGENESIS Sonic Hedgehog (Shh) Signaling Promotes Tumorigenicity and Stemness via Activation of Epithelial-to-Mesenchymal Transition (EMT) in Bladder Cancer S.S. Islam, 1,2 R.B. Mokhtari,

More information

Inhibition of E-Selectin or E-selectin together with CXCR4 Re-sensitizes Multiple Myeloma to Treatment

Inhibition of E-Selectin or E-selectin together with CXCR4 Re-sensitizes Multiple Myeloma to Treatment Inhibition of E-Selectin or E-selectin together with CXCR4 Re-sensitizes Multiple Myeloma to Treatment 1, Ph.D. Henna Bazai 1, Anita Sekula 1, William Fogler 2, Ted Smith 2, John Magnani 2 and Abdel Kareem

More information

Versican G3 Promotes Mouse Mammary Tumor Cell Growth, Migration, and Metastasis by Influencing EGF Receptor Signaling

Versican G3 Promotes Mouse Mammary Tumor Cell Growth, Migration, and Metastasis by Influencing EGF Receptor Signaling Versican G3 Promotes Mouse Mammary Tumor Cell Growth, Migration, and Metastasis by Influencing EGF Receptor Signaling William Weidong Du 1,2, Burton B. Yang 2, Tatiana A. Shatseva 2, Bing L. Yang 1,2,

More information

Tissues. tissue = many cells w/ same structure and function. cell shape aids its function tissue shape aids its function

Tissues. tissue = many cells w/ same structure and function. cell shape aids its function tissue shape aids its function Tissues tissue = many cells w/ same structure and function cell shape aids its function tissue shape aids its function Histology = study of tissues 4 types of tissues Epithelial coverings contact openings

More information

Molecular principles of cancer invasion and metastasis (Review)

Molecular principles of cancer invasion and metastasis (Review) INTERNATIONAL JOURNAL OF ONCOLOGY 34: 881-895, 2009 881 Molecular principles of cancer invasion and metastasis (Review) MATHIAS FELIX LEBER 1 and THOMAS EFFERTH 2 1 University of Mainz, Mainz; 2 German

More information

The Enemy Within: Human Cytomegalovirus. Juliet V. Spencer University of San Francisco

The Enemy Within: Human Cytomegalovirus. Juliet V. Spencer University of San Francisco The Enemy Within: Human Cytomegalovirus Juliet V. Spencer University of San Francisco NCASM Spring Meeting March 8, 2014 Herpes is all around us Human Herpesviruses Clinical Presentation Herpes simplex

More information

Cancer. The fundamental defect is. unregulated cell division. Properties of Cancerous Cells. Causes of Cancer. Altered growth and proliferation

Cancer. The fundamental defect is. unregulated cell division. Properties of Cancerous Cells. Causes of Cancer. Altered growth and proliferation Cancer The fundamental defect is unregulated cell division. Properties of Cancerous Cells Altered growth and proliferation Loss of growth factor dependence Loss of contact inhibition Immortalization Alterated

More information

Early Embryonic Development

Early Embryonic Development Early Embryonic Development Maternal effect gene products set the stage by controlling the expression of the first embryonic genes. 1. Transcription factors 2. Receptors 3. Regulatory proteins Maternal

More information

Chapter 11 Intercellular Communication and Tissue Architecture

Chapter 11 Intercellular Communication and Tissue Architecture Part III Organization of Cell Populations Chapter 11 Multicellular organisms such as the human body consist of various tissues such as epithelial tissues, bones and nerves, and organs such as heart and

More information

Laura Smart 9/22/2011

Laura Smart 9/22/2011 Laura Smart 9/22/2011 Fibrosis is a wound healing response in which damaged regions are encapsulated by an extracellular matrix or scar. Fibrosis develops in almost all patients with chronic liver injury

More information

Chapter 7: Membrane Structure & Function

Chapter 7: Membrane Structure & Function Chapter 7: Membrane Structure & Function 1. Membrane Structure 2. Transport Across Membranes 1. Membrane Structure Chapter Reading pp. 125-129 What are Biological Membranes? Hydrophilic head WATER They

More information

Chapter 7: Membrane Structure & Function. 1. Membrane Structure. What are Biological Membranes? 10/21/2015. Why phospholipids? 1. Membrane Structure

Chapter 7: Membrane Structure & Function. 1. Membrane Structure. What are Biological Membranes? 10/21/2015. Why phospholipids? 1. Membrane Structure Chapter 7: Membrane Structure & Function 1. Membrane Structure 2. Transport Across Membranes 1. Membrane Structure Chapter Reading pp. 125-129 What are Biological Membranes? Hydrophilic head WATER They

More information

Research Article 3507

Research Article 3507 Research Article 3507 Stromal fibroblasts from the interface zone of human breast carcinomas induce an epithelial mesenchymal transition-like state in breast cancer cells in vitro Ming-Qing Gao 1,2, Baek

More information