Cancer Biology Course. Invasion and Metastasis

Size: px
Start display at page:

Download "Cancer Biology Course. Invasion and Metastasis"

Transcription

1 Cancer Biology Course Invasion and Metastasis 2016 Lu-Hai Wang NHRI

2 Cancer metastasis Major problem: main reason for killing cancer patients, without it cancer can be cured or controlled. Challenging questions: Why cancer cells decide to move out? When do they move out? How do they do it? Where do they go and why? Cancer stem cells and metastasis Driving force? Timing? Mechanism? organtropism? role of stem cells?

3 Activity asso with brain is normal. Disseminated tumors Non-Hodgkin s lymphoma CAT :computed x-ray tomography plus PET: positron-emission tomography (FDG) Tumor cells take up FDG more than normal cells. Figure 14.1 The Biology of Cancer ( Garland Science 2007)

4 Pancreatic islet tumor cells from Rip-tag transgenic mouse metastasize to a lymphati c vessel Lymphatic vessel Figure 14.2a The Biology of Cancer ( Garland Science 2007)

5 Human breast cancer cells metastasize to a lymph node Figure 14.2b The Biology of Cancer ( Garland Science 2007)

6 Wright Giemsa stain of epithelial cancer cells in bone marrow Figure 14.2c The Biology of Cancer ( Garland Science 2007)

7 Figure 14.3 The Biology of Cancer ( Garland Science 2007) Primary tumor size and risk of metastasis

8 Figure 14.4 The Biology of Cancer ( Garland Science 2007) The invasion-metastasis cascade

9 Process of metastasis

10 more differentiated colorectal ca less differentiated colorectal ca Intact BM (green) (Basement membrane) lack BM (green)

11 Rectal carcinoma

12 The invasion pattern (migrating from left to right) of lobular mammary carcinoma cells Figure 14.5a The Biology of Cancer ( Garland Science 2007)

13 Pink/brown: Invading cervix squamous carcinoma cells into stroma Green: inflammatory cells Figure 14.5c The Biology of Cancer ( Garland Science 2007) pink arrows popint o basement membrane

14 Breast cancer line MCF-7 co-cultured with fibroblasts in 3-D gel Green: collagen matrix The migrating Cell cluster: Red: e-cadherin Green: actin Blue: cancer cells nuclei

15 Seen here is melanoma cancer cells migrating as a group. (confocal microscope image) Red: E-cadherin; green: beta-integrin Black: matrix digested away Figure 14.5b The Biology of Cancer ( Garland Science 2007)

16 Oral squamous carcinoma cells and ca-asso fibroblasts

17 Rip-Tag transgenic mouse pancreatic ca model Yellow: Macrophages stimulated by IL-4 and secreting cathepsins (red) at invasion front

18 Apc mutant mouse: intestinal carcinogenesis model CD34+ myeloidderived cells are recruited to cap the cancer cells at invasion front and secrete mmp2 and mmp9

19 Mena+ carcinoma cell, endothelial cell and macrophage from triad in tumor microenvironment for metastasis

20 Counting number of triad for prognosis

21 Intra-vitsal microscopy of caplillary and cells inside E: endothelial cell (capillary wall) R: erythrocyte W: leukocyte Figure 14.7a The Biology of Cancer ( Garland Science 2007)

22 Green: Capillary Red: erythrocyte Figure 14.7b The Biology of Cancer ( Garland Science 2007)

23 Figure 14.8 The Biology of Cancer ( Garland Science 2007) Cancer cells grow in the lumen of a microvessel.

24 Figure 14.6 The Biology of Cancer ( Garland Science 2007) Blood flow

25 Steps leading to extravasation Proteases digest away platelets. Cancer cells grow inside vessel. Cancer cells break through the capillary basement membrane Figure 14.9 The Biology of Cancer ( Garland Science 2007)

26 Colon carcinoma cells detected in bone marrow by anti-cytokeratin antibody Figure 14.10a The Biology of Cancer ( Garland Science 2007)

27 Breast cancer cells as micrometastasis detected in bone marrow Figure 14.10b The Biology of Cancer ( Garland Science 2007)

28 Two mouse lung adenocarcinoma micrometastases in a lymh node Figure 14.10c The Biology of Cancer ( Garland Science 2007)

29 Breast cancer Cytokeratin+ cells in marrow as prognosis indicator

30 Single cancer cells CGH to assess genetic heterogeneity of cancer cells from initial minimal residual diseases and those from subsequent metastatic relapse (from bone marrow) Figure 14.11a, b The Biology of Cancer ( Garland Science 2007)

31 Early stage: Heterogeneous micrometastases Late stage: Homogeneous metastases Figure 14.11c The Biology of Cancer ( Garland Science 2007)

32 ouse breast cancer cells injected into mesenteric vein traveled to liver, stayed dormant for weeks. Figure The Biology of Cancer ( Garland Science 2007)

33 EMT epithelial mesenchymal transition * EMT transcriptional factors: Twist, Snail, slug, Zeb1, Zeb2 etc *TGF-beta *Canonical Wnt signaling: E-Cadherin/beta-catenin *Non-canonical Wnt pathway: Win5a; Wnt/Ca signaling

34 Three types of EMT Kaluri and Weinberg, JCI (2009)

35 EMT Kaluri and Weinberg, JCI (2009)

36 Xin Ye and Robert A. Weinberg Trend in cell Biol,(2015)

37 Table 14.1 The Biology of Cancer ( Garland Science 2007)

38

39 Scanning E/M Sea urchin embryo Ectoderm cells migrate interially. Figure 14.13a The Biology of Cancer ( Garland Science 2007)

40 Figure 14.13b The Biology of Cancer ( Garland Science 2007)

Neoplasia 18 lecture 8. Dr Heyam Awad MD, FRCPath

Neoplasia 18 lecture 8. Dr Heyam Awad MD, FRCPath Neoplasia 18 lecture 8 Dr Heyam Awad MD, FRCPath ILOS 1. understand the angiogenic switch in tumors and factors that stimulate and inhibit angiogenesis. 2. list the steps important for tumor metastasis

More information

1.The metastatic cascade. 2.Pathologic features of metastasis. 3.Therapeutic ramifications

1.The metastatic cascade. 2.Pathologic features of metastasis. 3.Therapeutic ramifications Metastasis 1.The metastatic cascade 2.Pathologic features of metastasis 3.Therapeutic ramifications Sir James Paget (1814-1899) British Surgeon/ Pathologist Paget s disease of bone Paget s disease of the

More information

1. The metastatic cascade. 3. Pathologic features of metastasis. 4. Therapeutic ramifications. Which malignant cells will metastasize?

1. The metastatic cascade. 3. Pathologic features of metastasis. 4. Therapeutic ramifications. Which malignant cells will metastasize? 1. The metastatic cascade 3. Pathologic features of metastasis 4. Therapeutic ramifications Sir James Paget (1814-1899) British Surgeon/ Pathologist Paget s disease of Paget s disease of the nipple (intraductal

More information

number Done by Corrected by Doctor Maha Shomaf

number Done by Corrected by Doctor Maha Shomaf number 21 Done by Ahmad Rawajbeh Corrected by Omar Sami Doctor Maha Shomaf Ability to Invade and Metastasize The metastatic cascade can be subdivided into two phases: 1-invasion of ECM and vascular dissemination:

More information

Inflammatory Cells and Metastasis

Inflammatory Cells and Metastasis Inflammatory Cells and Metastasis Experimentelle Krebsforschung SS 07 Gerhard Christofori Institute of Biochemistry and Genetics Department of Clinical-Biological Sciences Center of Biomedicine University

More information

Invasion And Metastasis. Wirsma Arif Harahap Surgical Oncologist Surgery Department Andalas Medical Schoool

Invasion And Metastasis. Wirsma Arif Harahap Surgical Oncologist Surgery Department Andalas Medical Schoool Invasion And Metastasis Wirsma Arif Harahap Surgical Oncologist Surgery Department Andalas Medical Schoool Biology of tumor growth The natural history of malignant tumors can be divided into four phase:

More information

Tumor microenvironment Interactions and Lung Cancer Invasiveness. Pulmonary Grand Rounds Philippe Montgrain, M.D.

Tumor microenvironment Interactions and Lung Cancer Invasiveness. Pulmonary Grand Rounds Philippe Montgrain, M.D. Tumor microenvironment Interactions and Lung Cancer Invasiveness Pulmonary Grand Rounds Philippe Montgrain, M.D. February 26, 2009 Objectives Review epithelial mesenchymal transition (EMT), and its implications

More information

In vitro scratch assay: method for analysis of cell migration in vitro labeled fluorodeoxyglucose (FDG)

In vitro scratch assay: method for analysis of cell migration in vitro labeled fluorodeoxyglucose (FDG) In vitro scratch assay: method for analysis of cell migration in vitro labeled fluorodeoxyglucose (FDG) 1 Dr Saeb Aliwaini 13/11/2015 Migration in vivo Primary tumors are responsible for only about 10%

More information

Supplementary Materials. for Garmy-Susini, et al, Integrin 4 1 signaling is required for lymphangiogenesis and tumor metastasis

Supplementary Materials. for Garmy-Susini, et al, Integrin 4 1 signaling is required for lymphangiogenesis and tumor metastasis Supplementary Materials for Garmy-Susini, et al, Integrin 4 1 signaling is required for lymphangiogenesis and tumor metastasis 1 Supplementary Figure Legends Supplementary Figure 1: Integrin expression

More information

A holistic approach to targeting breast cancer part II: Micronutrient synergy. Presented by: Dr. Neha Shanker DRRI

A holistic approach to targeting breast cancer part II: Micronutrient synergy. Presented by: Dr. Neha Shanker DRRI A holistic approach to targeting breast cancer part II: Micronutrient synergy Presented by: Dr. Neha Shanker DRRI Overview of the previous webinar In the last presentation we talked about: Increase in

More information

Contents 1 The Windows of Susceptibility to Breast Cancer 2 The So Called Pre-Neoplastic Lesions and Carcinoma In Situ

Contents 1 The Windows of Susceptibility to Breast Cancer 2 The So Called Pre-Neoplastic Lesions and Carcinoma In Situ Contents 1 The Windows of Susceptibility to Breast Cancer... 1 1.1 Introduction... 1 1.2 Risk Factor and Etiological Agents... 2 1.3 The Concept of the Windows of Susceptibility to Carcinogenesis... 5

More information

Vaginal cancer: Know what to expect

Vaginal cancer: Know what to expect Vaginal cancer: Know what to expect For women with vaginal cancer What is the vagina? The vagina is a hollow canal that connects the cervix and the uterus to the outside. of the body. When a woman gives

More information

!!! Oncology for Scientists (RPN 530) Metastasis and Angiogenesis Chapter 13 and 14 Oct. 28 th 2014

!!! Oncology for Scientists (RPN 530) Metastasis and Angiogenesis Chapter 13 and 14 Oct. 28 th 2014 Oncology for Scientists (RPN 530) Metastasis and Angiogenesis Chapter 13 and 14 Oct. 28 th 2014 Department of Cancer Genetics Masashi Muramatsu, Ph.D. About Exam First, think and understand the concepts.

More information

Seeds and soil theory by Stephen Paget at the end of the XIX century.

Seeds and soil theory by Stephen Paget at the end of the XIX century. Seeds and soil theory by Stephen Paget at the end of the XIX century. In The Distribution Of Secondary Growths In Cancer Of The Breast Paget presents and analyzes 735 fatal cases of breast cancer, complete

More information

Cancer and Oncogenes Bioscience in the 21 st Century. Linda Lowe-Krentz

Cancer and Oncogenes Bioscience in the 21 st Century. Linda Lowe-Krentz Cancer and Oncogenes Bioscience in the 21 st Century Linda Lowe-Krentz December 1, 2010 Just a Few Numbers Becoming Cancer Genetic Defects Drugs Our friends and family 25 More mutations as 20 you get older

More information

Therapeutic targeting neuraminidase-1 in multi-stage of tumorigenesis

Therapeutic targeting neuraminidase-1 in multi-stage of tumorigenesis Therapeutic targeting neuraminidase-1 in multi-stage of tumorigenesis Myron R. Szewczuk Dept. Biomedical and Molecular Sciences, Queen's University, Kingston, K7L 3N6 Ontario, Canada HIGHLIGHTS. An innovative

More information

Heterotypy and Angiogenesis

Heterotypy and Angiogenesis Heterotypy and Angiogenesis Tumors are perpetual wounds 1. Normally stroma and epithelia converse at a distance. 2. Juxtaposition of stroma and epithelia is indicative of tissue damage. 4. Activate strategies

More information

ROLE OF TGF-BETA SIGNALING IN PIK3CA-

ROLE OF TGF-BETA SIGNALING IN PIK3CA- ROLE OF TGF-BETA SIGNALING IN - DRIVEN HEAD AND NECK CANCER INVASION AND METASTASIS 1,2 Sophia Bornstein, 3 Jingping Shen, 3 Jacob Minor, 3 Frank Hall, 3 Fang Zhang, 4 Sherif Said, 4 Xiao-Jing Wang, 1

More information

X-Plain Pancreatic Cancer Reference Summary

X-Plain Pancreatic Cancer Reference Summary X-Plain Pancreatic Cancer Reference Summary Introduction Pancreatic cancer is the 4th leading cause of cancer deaths in the U.S. About 37,000 new cases of pancreatic cancer are diagnosed each year in the

More information

The Role of Tenascin-C in Metastasis Formation

The Role of Tenascin-C in Metastasis Formation Chapter 3 The Role of Tenascin-C in Metastasis Formation Metastasis occurs when tumor cells spread from the primary site and form a new tumor at a different site within the same or another organ. This

More information

Supplementary Figure 1. HOPX is hypermethylated in NPC. (a) Methylation levels of HOPX in Normal (n = 24) and NPC (n = 24) tissues from the

Supplementary Figure 1. HOPX is hypermethylated in NPC. (a) Methylation levels of HOPX in Normal (n = 24) and NPC (n = 24) tissues from the Supplementary Figure 1. HOPX is hypermethylated in NPC. (a) Methylation levels of HOPX in Normal (n = 24) and NPC (n = 24) tissues from the genome-wide methylation microarray data. Mean ± s.d.; Student

More information

EMT: Epithelial Mesenchimal Transition

EMT: Epithelial Mesenchimal Transition EMT: Epithelial Mesenchimal Transition A phenotypic change that is characteristic of some developing tissues and certain forms of cancer. This is a multistep, key process in embryonic development and metastasis

More information

SSM signature genes are highly expressed in residual scar tissues after preoperative radiotherapy of rectal cancer.

SSM signature genes are highly expressed in residual scar tissues after preoperative radiotherapy of rectal cancer. Supplementary Figure 1 SSM signature genes are highly expressed in residual scar tissues after preoperative radiotherapy of rectal cancer. Scatter plots comparing expression profiles of matched pretreatment

More information

Cancer , The Patient Education Institute, Inc. ocf80101 Last reviewed: 06/08/2016 1

Cancer , The Patient Education Institute, Inc.   ocf80101 Last reviewed: 06/08/2016 1 Cancer Introduction Cancer begins in your cells, which are the building blocks of your body. Extra cells can form a mass called a tumor. Some tumors aren t cancerous, while other ones are. Cells from cancerous

More information

Tumour Structure and Nomenclature. Paul Edwards. Department of Pathology and Cancer Research UK Cambridge Institute, University of Cambridge

Tumour Structure and Nomenclature. Paul Edwards. Department of Pathology and Cancer Research UK Cambridge Institute, University of Cambridge Tumour Structure and Nomenclature Paul Edwards Department of Pathology and Cancer Research UK Cambridge Institute, University of Cambridge Malignant Metastasis Core idea of cancer Normal Cell Slightly

More information

CHAPTER VII CONCLUDING REMARKS AND FUTURE DIRECTION. Androgen deprivation therapy is the most used treatment of de novo or recurrent

CHAPTER VII CONCLUDING REMARKS AND FUTURE DIRECTION. Androgen deprivation therapy is the most used treatment of de novo or recurrent CHAPTER VII CONCLUDING REMARKS AND FUTURE DIRECTION Stathmin in Prostate Cancer Development and Progression Androgen deprivation therapy is the most used treatment of de novo or recurrent metastatic PCa.

More information

Cancers of unknown primary : Knowing the unknown. Prof. Ahmed Hossain Professor of Medicine SSMC

Cancers of unknown primary : Knowing the unknown. Prof. Ahmed Hossain Professor of Medicine SSMC Cancers of unknown primary : Knowing the unknown Prof. Ahmed Hossain Professor of Medicine SSMC Definition Cancers of unknown primary site (CUPs) Represent a heterogeneous group of metastatic tumours,

More information

Fundamental research on breast cancer in Belgium. Rosita Winkler

Fundamental research on breast cancer in Belgium. Rosita Winkler Fundamental research on breast cancer in Belgium Rosita Winkler Medline search for «breast cancer» and Belgium limits: english, posted in the last 5 years. Result: 484 papers - fundamental / clinical -

More information

Modulation of upa, MMPs and their inhibitors by a novel nutrient mixture in human colorectal, pancreatic and hepatic carcinoma cell lines

Modulation of upa, MMPs and their inhibitors by a novel nutrient mixture in human colorectal, pancreatic and hepatic carcinoma cell lines Modulation of upa, MMPs and their inhibitors by a novel nutrient mixture in human colorectal, pancreatic and hepatic carcinoma cell lines Publication from the Dr. Rath Research Institute International

More information

The 5th International Conference on Tumor Microenvironment: Progression, Therapy and Prevention Versailles, France, October 20 24, 2009

The 5th International Conference on Tumor Microenvironment: Progression, Therapy and Prevention Versailles, France, October 20 24, 2009 Cancer Microenvironment (2010) 3:1 5 DOI 10.1007/s12307-010-0039-2 EDITORIAL The 5th International Conference on Tumor Microenvironment: Progression, Therapy and Prevention Versailles, France, October

More information

ONCOLOGY. Csaba Bödör. Department of Pathology and Experimental Cancer Research november 19., ÁOK, III.

ONCOLOGY. Csaba Bödör. Department of Pathology and Experimental Cancer Research november 19., ÁOK, III. ONCOLOGY Csaba Bödör Department of Pathology and Experimental Cancer Research 2018. november 19., ÁOK, III. bodor.csaba1@med.semmelweis-univ.hu ONCOLOGY Characteristics of Benign and Malignant Neoplasms

More information

Vulvar cancer: Know what to expect

Vulvar cancer: Know what to expect Vulvar cancer: Know what to expect For women with vulvar cancer What is the vulva? The vulva is the external (outside) part of the female genitals. The vulva includes the outer and inner lip, the clitoris,

More information

Concomitant Gain of Function of Notch and Loss of p53 Signalling trigger an EMT Like Programme Driving Metastatic Intestinal Cancers

Concomitant Gain of Function of Notch and Loss of p53 Signalling trigger an EMT Like Programme Driving Metastatic Intestinal Cancers 8 AVRIl 2015 8 Avril 2015 Concomitant Gain of Function of Notch and Loss of p53 Signalling trigger an EMT Like Programme Driving Metastatic Intestinal Cancers Daniel LOUVARD UMR 144 / CNRS - Institut Curie

More information

NEOPLASIA. 3. Which of the following tumour is benign a. Chondrosarcoma b. Osteochondroma c. Chondroblastoma d. Ewing s tumour e.

NEOPLASIA. 3. Which of the following tumour is benign a. Chondrosarcoma b. Osteochondroma c. Chondroblastoma d. Ewing s tumour e. NEOPLASIA 1. malignant neoplasms a. are independent of hormonal influence b. are always composed of homogenous cell lines c. arise from differentiated cells by a process of anaplasia d. display abnormal

More information

HEPATIC METASTASES. We can state 3 types of metastases depending on their treatment options:

HEPATIC METASTASES. We can state 3 types of metastases depending on their treatment options: HEPATIC METASTASES 1. Definition Metastasis means the spread of cancer. Cancerous cells can separate from the primary tumor and enter the bloodstream or the lymphatic system (the one that produces, stores,

More information

Biochemistry of Carcinogenesis. Lecture # 35 Alexander N. Koval

Biochemistry of Carcinogenesis. Lecture # 35 Alexander N. Koval Biochemistry of Carcinogenesis Lecture # 35 Alexander N. Koval What is Cancer? The term "cancer" refers to a group of diseases in which cells grow and spread unrestrained throughout the body. It is difficult

More information

Index. Surg Oncol Clin N Am 16 (2007) Note: Page numbers of article titles are in boldface type.

Index. Surg Oncol Clin N Am 16 (2007) Note: Page numbers of article titles are in boldface type. Surg Oncol Clin N Am 16 (2007) 465 469 Index Note: Page numbers of article titles are in boldface type. A Adjuvant therapy, preoperative for gastric cancer, staging and, 339 B Breast cancer, metabolic

More information

General Information Key Points

General Information Key Points The content of this booklet was adapted from content originally published by the National Cancer Institute. Male Breast Cancer Treatment (PDQ ) Patient Version. Updated September 29,2017. https://www.cancer.gov/types/breast/patient/male-breast-treatment-pdq

More information

BY Mrs. K.SHAILAJA., M. PHARM., LECTURER DEPT OF PHARMACY PRACTICE, SRM COLLEGE OF PHARMACY

BY Mrs. K.SHAILAJA., M. PHARM., LECTURER DEPT OF PHARMACY PRACTICE, SRM COLLEGE OF PHARMACY BY Mrs. K.SHAILAJA., M. PHARM., LECTURER DEPT OF PHARMACY PRACTICE, SRM COLLEGE OF PHARMACY Cancer is a group of more than 100 different diseases that are characterized by uncontrolled cellular growth,

More information

Neoplasia 18 lecture 6. Dr Heyam Awad MD, FRCPath

Neoplasia 18 lecture 6. Dr Heyam Awad MD, FRCPath Neoplasia 18 lecture 6 Dr Heyam Awad MD, FRCPath ILOS 1. understand the role of TGF beta, contact inhibition and APC in tumorigenesis. 2. implement the above knowledge in understanding histopathology reports.

More information

PBS Class #2 Introduction to the Immune System part II Suggested reading: Abbas, pgs , 27-30

PBS Class #2 Introduction to the Immune System part II Suggested reading: Abbas, pgs , 27-30 PBS 803 - Class #2 Introduction to the Immune System part II Suggested reading: Abbas, pgs. 15-25, 27-30 Learning Objectives Compare and contrast the maturation of B and T lymphocytes Compare and contrast

More information

MIT Student EMT variations in cancer

MIT Student EMT variations in cancer MIT Student EMT variations in cancer Epithelial mesenchymal transition is a transformative process that normal cells, as well as cancer cells, undertake, Throughout the life cycle of a tumor, the environmental

More information

Acute: Symptoms that start and worsen quickly but do not last over a long period of time.

Acute: Symptoms that start and worsen quickly but do not last over a long period of time. Cancer Glossary Acute: Symptoms that start and worsen quickly but do not last over a long period of time. Adjuvant therapy: Treatment given after the main treatment. It usually refers to chemotherapy,

More information

TITLE: Microenvironments and Signaling Pathways Regulating Early Dissemination, Dormancy, and Metastasis

TITLE: Microenvironments and Signaling Pathways Regulating Early Dissemination, Dormancy, and Metastasis AWARD NUMBER: W81XWH-14-1-0296 TITLE: Microenvironments and Signaling Pathways Regulating Early Dissemination, Dormancy, and Metastasis PRINCIPAL INVESTIGATOR: John Condeelis CONTRACTING ORGANIZATION:

More information

(A) RT-PCR for components of the Shh/Gli pathway in normal fetus cell (MRC-5) and a

(A) RT-PCR for components of the Shh/Gli pathway in normal fetus cell (MRC-5) and a Supplementary figure legends Supplementary Figure 1. Expression of Shh signaling components in a panel of gastric cancer. (A) RT-PCR for components of the Shh/Gli pathway in normal fetus cell (MRC-5) and

More information

What is Cancer? Understanding the basics of

What is Cancer? Understanding the basics of What is Cancer? Understanding the basics of A process of physical and biological changes that occur when normal healthy cells are transformed into cancer cells What is Cancer? Cancer is an umbrella term

More information

SIBLINGs, cancer's multifunctional weapons

SIBLINGs, cancer's multifunctional weapons SIBLINGs, cancer's multifunctional weapons 6/18/08 Akeila Bellahcène and Vincent Castronovo of the Metastasis Research laboratory of the University of Liège are among the first researchers to have discovered

More information

Chapter 10 Breast Cancer Liver Metastasis

Chapter 10 Breast Cancer Liver Metastasis Chapter 10 Breast Cancer Liver Metastasis Sébastien Tabariès and Peter M. Siegel Abstract The emergence of metastatic breast cancer is the most deadly aspect of this disease and once it has spread from

More information

Sheet #9. Dr. Heba Kalbouneh. Dr. Heba Kalbouneh. Dr. Heba Kalbouneh

Sheet #9. Dr. Heba Kalbouneh. Dr. Heba Kalbouneh. Dr. Heba Kalbouneh Sheet #9 Dr. Heba Kalbouneh Dr. Heba Kalbouneh Dr. Heba Kalbouneh Elastic fibers The main function of elastic fibers is to provide elasticity. In other words these fibers are able to restore the original

More information

VIII Curso Internacional del PIRRECV. Some molecular mechanisms of cancer

VIII Curso Internacional del PIRRECV. Some molecular mechanisms of cancer VIII Curso Internacional del PIRRECV Some molecular mechanisms of cancer Laboratorio de Comunicaciones Celulares, Centro FONDAP Estudios Moleculares de la Celula (CEMC), ICBM, Facultad de Medicina, Universidad

More information

LYMPHATIC DRAINAGE IN THE HEAD & NECK

LYMPHATIC DRAINAGE IN THE HEAD & NECK LYMPHATIC DRAINAGE IN THE HEAD & NECK Like other parts of the body, the head and neck contains lymph nodes (commonly called glands). Which form part of the overall Lymphatic Drainage system of the body.

More information

Biochimica et Biophysica Acta

Biochimica et Biophysica Acta Biochimica et Biophysica Acta 1796 (2009) 293 308 Contents lists available at ScienceDirect Biochimica et Biophysica Acta journal homepage: www.elsevier.com/locate/bbacan Review Metastasis mechanisms Thomas

More information

Supporting Information

Supporting Information Supporting Information Chan et al. 1.173/pnas.9654916 A Patient B Xenograft C * remaining feature of normal lymph node * * * D lymphocytes Infiltrating transitional carcinoma cells E Enlarged axillary

More information

The Hallmarks of Cancer

The Hallmarks of Cancer The Hallmarks of Cancer Theresa L. Hodin, Ph.D. Clinical Research Services Theresa.Hodin@RoswellPark.org Hippocrates Cancer surgery, circa 1689 Cancer Surgery Today 1971: Nixon declares War on Cancer

More information

(a) Significant biological processes (upper panel) and disease biomarkers (lower panel)

(a) Significant biological processes (upper panel) and disease biomarkers (lower panel) Supplementary Figure 1. Functional enrichment analyses of secretomic proteins. (a) Significant biological processes (upper panel) and disease biomarkers (lower panel) 2 involved by hrab37-mediated secretory

More information

Breast Cancer Diagnosis, Treatment and Follow-up

Breast Cancer Diagnosis, Treatment and Follow-up Breast Cancer Diagnosis, Treatment and Follow-up What is breast cancer? Each of the body s organs, including the breast, is made up of many types of cells. Normally, healthy cells grow and divide to produce

More information

Role of Cancer Microenvironment in Metastasis: Focus on Colon Cancer

Role of Cancer Microenvironment in Metastasis: Focus on Colon Cancer Cancer Microenvironment (2008) 1:69 83 DOI 10.1007/s12307-008-0007-2 REVIEW PAPER Role of Cancer Microenvironment in Metastasis: Focus on Colon Cancer Stéphanie Gout & Jacques Huot Received: 22 December

More information

Liquid Biopsy: Implications for Cancer Staging & Therapy

Liquid Biopsy: Implications for Cancer Staging & Therapy Prof. Klaus Pantel, MD, PhD Institut für Tumorbiologie Liquid Biopsy: Implications for Cancer Staging & Therapy Tumor cell dissemination and cancer dormancy Primary tumor Local relapse Cancer cells disseminate

More information

Tissue repair. (3&4 of 4)

Tissue repair. (3&4 of 4) Tissue repair (3&4 of 4) What will we discuss today: Regeneration in tissue repair Scar formation Cutaneous wound healing Pathologic aspects of repair Regeneration in tissue repair Labile tissues rapid

More information

Clinical indications for positron emission tomography

Clinical indications for positron emission tomography Clinical indications for positron emission tomography Oncology applications Brain and spinal cord Parotid Suspected tumour recurrence when anatomical imaging is difficult or equivocal and management will

More information

General information about skin cancer

General information about skin cancer Skin Cancer General information about skin cancer Key points Skin cancer is a disease in which malignant (cancer) cells form in the tissues of the skin. There are different types of cancer that start in

More information

Lymphoid System: cells of the immune system. Answer Sheet

Lymphoid System: cells of the immune system. Answer Sheet Lymphoid System: cells of the immune system Answer Sheet Q1 Which areas of the lymph node have most CD3 staining? A1 Most CD3 staining is present in the paracortex (T cell areas). This is towards the outside

More information

The Angiopoietin Axis in Cancer

The Angiopoietin Axis in Cancer Ang2 Ang1 The Angiopoietin Axis in Cancer Tie2 An Overview: The Angiopoietin Axis Plays an Essential Role in the Regulation of Tumor Angiogenesis Growth of a tumor beyond a limiting size is dependent upon

More information

Disclosure of Relevant Financial Relationships. Breast Pathology Evening Specialty Conference Case #4. Clinical Case: Pathologic Features

Disclosure of Relevant Financial Relationships. Breast Pathology Evening Specialty Conference Case #4. Clinical Case: Pathologic Features Breast Pathology Evening Specialty Conference Case #4 K.P. Siziopikou, MD, PhD Professor of Pathology Director of Breast Pathology and Breast Pathology Fellowship Program Northwestern University Feinberg

More information

Gastric (Stomach) Cancer

Gastric (Stomach) Cancer Gastric (Stomach) Cancer Gastric cancer is a disease in which malignant (cancer) cells form in the lining of the stomach. The stomach is a J-shaped organ in the upper abdomen. It is part of the digestive

More information

Computational Systems Biology: Biology X

Computational Systems Biology: Biology X Bud Mishra Room 1002, 715 Broadway, Courant Institute, NYU, New York, USA L#5:(October-18-2010) Cancer and Signals Outline 1 2 Outline 1 2 Cancer is a disease of malfunctioning cells. Cell Lineage: Adult

More information

CODING TUMOUR MORPHOLOGY. Otto Visser

CODING TUMOUR MORPHOLOGY. Otto Visser CODING TUMOUR MORPHOLOGY Otto Visser INTRODUCTION The morphology describes the tissue of the tumour closest to normal tissue Well differentiated tumours are closest to normal Undifferentiated tumours show

More information

Epithelial mesenchymal plasticity in carcinoma metastasis

Epithelial mesenchymal plasticity in carcinoma metastasis REVIEW Epithelial mesenchymal plasticity in carcinoma metastasis Jeff H. Tsai 1 and Jing Yang 1,2,3 1 Department of Pharmacology, 2 Department of Pediatrics, School of Medicine, University of California

More information

Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma

Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma EXPERIMENTAL AND THERAPEUTIC MEDICINE 6: 1489-1493, 2013 Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma JINQUAN CAI Department of Urology, Fuzhou

More information

Mathematics and Physics of Cancer: Questions. Robijn Bruinsma, UCLA KITP Colloquium May 6, ) Cancer statistics and the multi-stage model.

Mathematics and Physics of Cancer: Questions. Robijn Bruinsma, UCLA KITP Colloquium May 6, ) Cancer statistics and the multi-stage model. Mathematics and Physics of Cancer: Questions Robijn Bruinsma, UCLA KITP Colloquium May 6, 2009 1) Cancer statistics and the multi-stage model. 2) Cancer microevolution and clonal expansion. 3) Metastasis:

More information

Esophageal cancer. What is esophageal cancer? Esophageal cancer is a disease in which malignant (cancer) cells form in the tissues of the esophagus.

Esophageal cancer. What is esophageal cancer? Esophageal cancer is a disease in which malignant (cancer) cells form in the tissues of the esophagus. Esophageal Cancer Esophageal cancer What is esophageal cancer? What are risk factors? Signs and symptoms Tests for esophageal cancer Stages of esophageal cancer Treatment options What is esophageal cancer?

More information

A Novel CTC-Detecting Technique Using TelomeScan and Its Clinical Applications

A Novel CTC-Detecting Technique Using TelomeScan and Its Clinical Applications A Novel CTC-Detecting Technique Using TelomeScan and Its Clinical Applications Yasuo Urata CEO and President Oncolys BioPharma Inc. February 16, 2013 Telomere Length is a Limiting Factor for Cell Replication

More information

Cancer. Chapter 31 Lesson 2

Cancer. Chapter 31 Lesson 2 Cancer Chapter 31 Lesson 2 Tumors All cancers are tumors- masses of tissue. Not all tumors are cancers. Some tumors are benign- noncancerous. These tumors are surrounded by membranes that prevent them

More information

Oncology 101. Cancer Basics

Oncology 101. Cancer Basics Oncology 101 Cancer Basics What Will You Learn? What is Cancer and How Does It Develop? Cancer Diagnosis and Staging Cancer Treatment What is Cancer? Cancer is a group of more than 100 different diseases

More information

SUPPLEMENTARY INFORMATION

SUPPLEMENTARY INFORMATION b 350 300 250 200 150 100 50 0 E0 E10 E50 E0 E10 E50 E0 E10 E50 E0 E10 E50 Number of organoids per well 350 300 250 200 150 100 50 0 R0 R50 R100 R500 1st 2nd 3rd Noggin 100 ng/ml Noggin 10 ng/ml Noggin

More information

Cancer therapeutics in the clinical setting. Need for alternate therapeutics. Target multiple cancer pathways. Circumvent the genetic mutations

Cancer therapeutics in the clinical setting. Need for alternate therapeutics. Target multiple cancer pathways. Circumvent the genetic mutations Transcriptional factor Snail and MMP-9 signaling axis controls tumor neovascularization, growth and metastasis in mouse model of human ovarian carcinoma Samar Abdulkhalek 1, Olivia Geen 1, Lacey Brodhagen

More information

Effect of a nutrient mixture on the localization of extracellular matrix proteins in HeLa human cervical cancer xenografts in female nude mice

Effect of a nutrient mixture on the localization of extracellular matrix proteins in HeLa human cervical cancer xenografts in female nude mice Effect of a nutrient mixture on the localization of extracellular matrix proteins in HeLa human cervical cancer xenografts in female nude mice Publication from the Dr. Rath Research Institute Experimental

More information

PATHOBIOLOGY OF NEOPLASIA

PATHOBIOLOGY OF NEOPLASIA PATHOBIOLOGY OF NEOPLASIA Department of Pathology Gadjah Mada University School of Medicine dr. Harijadi Blok Biomedis, 6 Maret 2009 [12] 3/17/2009 1 The pathobiology of neoplasia Normal cells Malignant

More information

Dr. Heba Kalbouneh. Dr. Heba Kalbouneh. Dr. Heba Kalbouneh

Dr. Heba Kalbouneh. Dr. Heba Kalbouneh. Dr. Heba Kalbouneh Dr. Heba Kalbouneh Dr. Heba Kalbouneh Dr. Heba Kalbouneh Basement membrane: What is the basement membrane? - It is a layer of ECM separating the epithelial cells from the underlying connective tissue Basement

More information

Disclosures. Outline. What IS tumor budding?? Tumor Budding in Colorectal Carcinoma: What, Why, and How. I have nothing to disclose

Disclosures. Outline. What IS tumor budding?? Tumor Budding in Colorectal Carcinoma: What, Why, and How. I have nothing to disclose Tumor Budding in Colorectal Carcinoma: What, Why, and How Disclosures I have nothing to disclose Soo-Jin Cho, MD, PhD Assistant Professor UCSF Dept of Pathology Current Issues in Anatomic Pathology 2017

More information

An epithelial-to-mesenchymal transition-inducing potential of. granulocyte macrophage colony-stimulating factor in colon. cancer

An epithelial-to-mesenchymal transition-inducing potential of. granulocyte macrophage colony-stimulating factor in colon. cancer An epithelial-to-mesenchymal transition-inducing potential of granulocyte macrophage colony-stimulating factor in colon cancer Yaqiong Chen, Zhi Zhao, Yu Chen, Zhonglin Lv, Xin Ding, Renxi Wang, He Xiao,

More information

Management of Neck Metastasis from Unknown Primary

Management of Neck Metastasis from Unknown Primary Management of Neck Metastasis from Unknown Primary.. Definition Histologic evidence of malignancy in the cervical lymph node (s) with no apparent primary site of original tumour Diagnosis after a thorough

More information

肿瘤信息学 徐鹰佐治亚大学生化系 吉林大学计算机学院

肿瘤信息学 徐鹰佐治亚大学生化系 吉林大学计算机学院 肿瘤信息学 徐鹰佐治亚大学生化系 吉林大学计算机学院 2 Lecture V Biology of cancer metastasis and metastatic cancers 3 Metastatic Cancer Metastatic cancers are responsible for 90% of cancer related death Currently metastatic cancer

More information

Anemia: A condition in which the number of red blood cells is below normal.

Anemia: A condition in which the number of red blood cells is below normal. Veterinary Teaching Hospital Oncology Service Virginia-Maryland Regional College of Veterinary Medicine 245 Duck Pond Drive Blacksburg, VA 24061 Phone: 540-231-4621 Definitions This fact sheet is based

More information

OBJECTIVES. 1. List the major hallmarks of cancer. 2. Relate specific genes/proteins to individual hallmarks

OBJECTIVES. 1. List the major hallmarks of cancer. 2. Relate specific genes/proteins to individual hallmarks OBJECTIVES 1. List the major hallmarks of cancer 2. Relate specific genes/proteins to individual hallmarks 3. Explain how hallmarks of cancer lead to cancer development Case Study 60 year old female Previously

More information

Impact of NM23-M1 "knock-out" on metastatic potential of hepatocellular carcinoma: a transgenic approach

Impact of NM23-M1 knock-out on metastatic potential of hepatocellular carcinoma: a transgenic approach Impact of NM23-M1 "knock-out" on metastatic potential of hepatocellular carcinoma: a transgenic approach NM23-M1 "knock- out" mice (without NDPK A) Experimental models of hepatocarcinogenesis Chemical

More information

Basement membrane in lobule.

Basement membrane in lobule. Bahram Memar, MD Basement membrane in lobule. Normal lobule-luteal phase Normal lobule-follicular phase Lactating breast Greater than 95% are adenocarcinomas in situ carcinomas and invasive carcinomas.

More information

Real-time imaging reveals the single steps of brain metastasis fo mation r

Real-time imaging reveals the single steps of brain metastasis fo mation r Real-time imaging reveals the single steps of brain metastasis fo mation r Yvonne Kienast, Louisa von Baumgarten, Martin Fuhrmann, Wolfgang E.F. Klinkert, Roland Goldbrunner, Jochen Herms and Frank Winkler

More information

Macrophages form functional vascular mimicry channels in vivo. SI Figures and Legend

Macrophages form functional vascular mimicry channels in vivo. SI Figures and Legend Macrophages form functional vascular mimicry channels in vivo Authors: *Faith H. Barnett, *Mauricio Rosenfeld, Malcolm Wood, William Kiosses, Yoshihiko Usui, Valentina Marchetti, Edith Aguilar, and Martin

More information

We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists. International authors and editors

We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists. International authors and editors We are IntechOpen, the world s leading publisher of Open Access books Built by scientists, for scientists 4,000 116,000 120M Open access books available International authors and editors Downloads Our

More information

Supplementary Figure 1. Characterization of NMuMG-ErbB2 and NIC breast cancer cells expressing shrnas targeting LPP. NMuMG-ErbB2 cells (a) and NIC

Supplementary Figure 1. Characterization of NMuMG-ErbB2 and NIC breast cancer cells expressing shrnas targeting LPP. NMuMG-ErbB2 cells (a) and NIC Supplementary Figure 1. Characterization of NMuMG-ErbB2 and NIC breast cancer cells expressing shrnas targeting LPP. NMuMG-ErbB2 cells (a) and NIC cells (b) were engineered to stably express either a LucA-shRNA

More information

Angiogenesis and Metastasis

Angiogenesis and Metastasis Angiogenesis and Metastasis 10/31/2017 Tariq Bhat (Ph.D.) Definition of Angiogenesis: Formation of new blood vessels/capillaries from the pre-existing vessels/capillaries Involves - sprouting - splitting

More information

Supplementary Figure 1. Deletion of Smad3 prevents B16F10 melanoma invasion and metastasis in a mouse s.c. tumor model.

Supplementary Figure 1. Deletion of Smad3 prevents B16F10 melanoma invasion and metastasis in a mouse s.c. tumor model. A B16F1 s.c. Lung LN Distant lymph nodes Colon B B16F1 s.c. Supplementary Figure 1. Deletion of Smad3 prevents B16F1 melanoma invasion and metastasis in a mouse s.c. tumor model. Highly invasive growth

More information

Neoplasia part I. Dr. Mohsen Dashti. Clinical Medicine & Pathology nd Lecture

Neoplasia part I. Dr. Mohsen Dashti. Clinical Medicine & Pathology nd Lecture Neoplasia part I By Dr. Mohsen Dashti Clinical Medicine & Pathology 316 2 nd Lecture Lecture outline Review of structure & function. Basic definitions. Classification of neoplasms. Morphologic features.

More information

Correlation between expression and significance of δ-catenin, CD31, and VEGF of non-small cell lung cancer

Correlation between expression and significance of δ-catenin, CD31, and VEGF of non-small cell lung cancer Correlation between expression and significance of δ-catenin, CD31, and VEGF of non-small cell lung cancer X.L. Liu 1, L.D. Liu 2, S.G. Zhang 1, S.D. Dai 3, W.Y. Li 1 and L. Zhang 1 1 Thoracic Surgery,

More information

performed to help sway the clinician in what the appropriate diagnosis is, which can substantially alter the treatment of management.

performed to help sway the clinician in what the appropriate diagnosis is, which can substantially alter the treatment of management. Hello, I am Maura Polansky at the University of Texas MD Anderson Cancer Center. I am a Physician Assistant in the Department of Gastrointestinal Medical Oncology and the Program Director for Physician

More information

Pancreas Quizzes c. Both A and B a. Directly into the blood stream (not using ducts)

Pancreas Quizzes c. Both A and B a. Directly into the blood stream (not using ducts) Pancreas Quizzes Quiz 1 1. The pancreas produces hormones. Which type of hormone producing organ is the pancreas? a. Endocrine b. Exocrine c. Both A and B d. Neither A or B 2. Endocrine indicates hormones

More information

Radiology Pathology Conference

Radiology Pathology Conference Radiology Pathology Conference Sharlin Johnykutty,, MD, Cytopathology Fellow Sara Majewski, MD, Radiology Resident Friday, August 28, 2009 Presentation material is for education purposes only. All rights

More information

WELLNESS INITIATIVE NOW

WELLNESS INITIATIVE NOW WELLNESS INITIATIVE NOW To promote personal well-being, fitness and nutrition for all TDCJ employees. November 2008 Lung cancer is cancer that forms in tissues of the lung, usually in the cells lining

More information

Prepared By Student. Dania Abed Al-majeed. Rahma Raad Hanna. Balqees Mohammed Aasim. Dania Hisham. Rasha Rafiee

Prepared By Student. Dania Abed Al-majeed. Rahma Raad Hanna. Balqees Mohammed Aasim. Dania Hisham. Rasha Rafiee Prepared By Student Rahma Raad Hanna Balqees Mohammed Aasim Dania Hisham Dania Abed Al-majeed Rasha Rafiee Epithelia Epithelia can be derived from ectoderm, mesoderm or endoderm -ectoderm gives rise to

More information