Supplementary Figure S1 Supplementary Figure S2
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1 Supplementary Figure S A) The blots shown in Figure B were qualified by using Gel-Pro analyzer software (Rockville, MD, USA). The ratio of LC3II/LC3I to actin was then calculated. The data are represented as mean±sd from three independent experiments. B) The blots in Figure E were qualified and the ratio of LC3II/LC3I to actin was then calculated. The data are represented as mean±sd from three independent experiments. C) HCT cells with stable expression of GFP-LC3 were treated with Embelin as indicated. GFP-LC3 puncta accumulation was observed by a microscope. Quantification of LC3 punctate cells was shown on the right. The data are represented as means±sd of three independent experiments. D-G) The blots in Figures G, H, I and J were qualified and the ratio of LC3II/LC3I to actin was then calculated and shown in D, E, F and G, respectively. The data are represented as mean±sd from three independent experiments. Supplementary Figure S A) The blots in Figure A were qualified and the ratio of LC3II/LC3I to actin was then calculated. The data are represented as mean±sd from three independent experiments. B) MCF7, MCFA, HepG and LO cells were individually treated with specific or control sirnas. 8 h after transfection, cell lysates were analyzed by Western blotting with the indicated antibodies. The data are representative of two biological replicates. C) HCT cells were infected with lentiviruses expressing control shrna or 3 different sets of specific shrnas. 8 h after infection, cell lysates were subjected to Western blot analysis with the indicated antibodies. The data are representative of three biological replicates. D-F) The blots in Figures B, D and E were qualified and the ratio of LC3II/LC3I to actin was then calculated and shown in D, E and F, respectively. The data are represented as mean±sd from three independent experiments.
2 G) HCT KO cells were transfected with Flag--WT (wild type) or the indicated mutants. h after transfection, cell lysates were analyzed by Western blotting with the indicated antibodies. The data are representative of two biological replicates. H) The blots in Figure F were qualified and the ratio of LC3II/LC3I to actin was then calculated. The data are represented as mean±sd from three independent experiments. Supplementary Figure S3 A) Lysates from HCT cells were immunoprecipitated with anti- antibody or an isotope-matched control antibody. Immunoprecipitates and cell lysates were analyzed by Western blotting. The data are representative of two biological replicates. B) The blots in Figure 3C were qualified and the ratio of to actin was then calculated. The data are represented as mean±sd from three independent experiments. C and D) HCT WT and KO cells were treated with 5μg ml - cycloheximide for the indicated periods of time. The half-life of was measured by Western blot analysis (C). We should mention that amounts of cell lysates were adjusted to achieve similar expression levels of at time. The blots were qualified and the ratio of to actin was then calculated. The data are represented as mean±sd from three independent experiments (D). E) HCT KO cells were transfected with and constructs as indicated. h after transfection, cells were treated with 5μg ml - cycloheximide for the indicated periods of time followed by western blot analysis with the indicated antibodies. The data are representative of three biological replicates. Supplementary Figure S A) Lysates from HEK 93T cells expressing HA- alone or HA- plus Flag- were immunoprecipitated with anti-flag antibody. Immunoprecipitates were then analyzed by Western blotting. The data are representative of two biological
3 replicates. B) HCT KO cells were transfected with HA- alone or HA- plus Flag- D8A/W3A. h after transfection, cells were treated with CHX for the indicated periods of time. Cell lysates were then analyzed by Western blotting. The blots were qualified and the ratio of HA- to was then calculated. The data are represented as mean±sd from three independent experiments C) Lysates from HEK 93T cells expressing HA- alone or HA- plus Flag- H7A were immunoprecipitated with anti-flag antibody followed by Western blot analysis. The data are representative of two biological replicates. Supplementary Figure S5 A) Purified Flag- CA was incubated with increasing amounts of His- recombinant protein in a total of µl in vitro ubiquitination reaction buffer at 37 C for h. The reaction mixtures were analyzed by Western blotting with anti- antibody. The data are representative of three biological replicates. B) HCT cells expressing specific or control sirnas were treated with µm MG-3 for h. Cell lysates were denatured before immunoprecipitation with anti- and anti- antibodies. Immunoprecipitates were analyzed by Western blotting with anti-ubiquitin antibody. The data are representative of three biological replicates. C) -/- -/- MEF cells were transfected with the indicated plasmids. h after transfection, cell lysates were immunoprecipitated with anti-ha antibody. The input and immunoprecipitates were analyzed by Western blotting. The data are representative of two biological replicates. Supplementary Figure S A) HCT WT and KO cells were treated with EBSS for the indicated periods of time. Cell lysates were subjected to Western blot analysis with the indicated antibodies. The data are representative of three biological replicates. B-D) The blots in Figures B, C and F were qualified and the ratio of LC3II/LC3I 3
4 to actin was then calculated and shown in B, C and D, respectively. The data are represented as mean±sd from three independent experiments. Supplementary Figure S7 Expression levels of,, and LC3 conversion in the tumors excised from indicated different groups of mice (Figure 5A) were evaluated by Western blot analysis. Left and Right indicate tumors grown in the left and right flanks of mice, respectively. The data are representative of two biological replicates. Supplementary Figure S8 Histological sections of the indicated xenograft tumors were stained with the indicated antibodies. Supplementary Figure S9 A and B) The blots in Figures 5G and 5H were qualified and the ratio of LC3II/LC3I to actin was then calculated and shown in A and B, respectively. The data are represented as mean±sd from three independent experiments. Supplementary Figure S The tumor tissues (T) from human Breast, Lung and Stomach and their adjacent normal tissues (N) were homogenized for protein extraction. Protein extracts were analyzed by Western blotting with the indicated antibodies. As shown in the table, 5 of 3 esophagus cancers (3%), 3 of 5 intestine cancers (%), of 3 breast cancers (33%), of 8 lung cancers (5%), and 3 of stomach cancers (3%) exhibited the great autophagy inhibition along with the elevated expression of p-,, and and the decreased expression of compared to those from their matched adjacent normal tissues. The data are representative of three biological replicates. Supplementary Figure S Histological sections of the tumor tissues from human Breast, Colon, Esophagus,
5 Stomach, and Lung and their adjacent normal tissues were stained with the indicated antibodies. 5
6 A C LC3II / LC3I / 8 LC3II / LC3I / HCT GFP-hLC3 + Embelin ng/ml 5ng/ml 3ng/ml ng/ml B Figure S h 3ng/ml h h h h Cells with GFP-LC3 punctation (%) 3 (ng/ml) (h) h 3ng/ml 5 3 D E LC3II / LC3I / LC3II / LC3I / 8 3 F G. LC3II / LC3I / LC3II / LC3I /..8...
7 A LC3II / LC3I / C HCT Sh-Ctrl + Sh- + Sh- + Sh-3 + E F H 8 LC3II / LC3I / LC3II / LC3I / LC3II / LC3I / LC3 I LC3 II B MCF7 MCFA HepG LO Si-Ctrl Si D LC3II / LC3I / G 5 Flag R75H KO R73H G79E K38R WT 3 5 LC3 I LC3 II PARP p5/7 Figure S LC3 I LC3 II Procaspase3
8 A Input C HCT WT KO CHX (min) IP: anti-igg IP: anti B D / / Figure S3 E KO + HA- WT Flag Flag- WT Flag- H7A CHX (min) HA- Flag GFP KO + HA- CA Flag Flag- WT Flag- H7A CHX (min) HA- Flag GFP
9 A HA- Flag- D8A/W3A Input HA- Flag- Ip: anti-flag Figure S B HCT KO HA Flag- D8A/W3A CHX (min) HA- Flag- / C HA- Flag- H7A Input HA- Flag- Ip: anti-flag
10 Figure S5 A B HCT E+E+Ub His- Flag- CA Si-Ctrl Si- C HA- GFP- Flag- -(Ub)n His- Input IP: anti-ha HA- GFP- +MG3 Input IP IP: anti- IP: anti (Ub)n -(Ub)n Flag-
11 Figure S A HCT WT KO EBSS 8 8 (h) LC3 I LC3 II C LC3II / LC3I / B LC3II / LC3I / LC3II / LC3I / D
12 Figure S7 Left: WT Right: KO LC3I LC3II 3 Left: KO-Ctrl Right: KO- SA LC3I LC3II Left: KO-Ctrl Right: KO- Left: KO-Ctrl Right: KO- SD LC3I LC3II LC3I LC3II
13 Figure S8 WT KO KO- KO- SA KO- SD LC3
14 Figure S9 A LC3II / LC3I / B LC3II / LC3I /..8...
15 Figure S Breast Lung Stomach N T N T N T N T N T N3 T3 p- LC3I LC3II 3 p- LC3I LC3II 3 5 p- LC3I LC3II Esophagus Intestine Breast Lung Stomach 3% ( 5 / ) % ( 3 / 5 ) 33% ( / 3 ) 5% ( / 8 ) 3% ( 3 / )
16 Cancer Lung Normal Cancer Normal Stomach Cancer Normal Esophagus Cancer Colon Normal Cancer Normal Breast LC3 Figure S
RAW264.7 cells stably expressing control shrna (Con) or GSK3b-specific shrna (sh-
1 a b Supplementary Figure 1. Effects of GSK3b knockdown on poly I:C-induced cytokine production. RAW264.7 cells stably expressing control shrna (Con) or GSK3b-specific shrna (sh- GSK3b) were stimulated
SUPPLEMENTAL FIGURE LEGENDS
SUPPLEMENTAL FIGURE LEGENDS Supplemental Figure S1: Endogenous interaction between RNF2 and H2AX: Whole cell extracts from 293T were subjected to immunoprecipitation with anti-rnf2 or anti-γ-h2ax antibodies
Supplementary Figure 1. MAT IIα is Acetylated at Lysine 81.
IP: Flag a Mascot PTM Modified Mass Error Position Gene Names Score Score Sequence m/z [ppm] 81 MAT2A;AMS2;MATA2 35.6 137.28 _AAVDYQK(ac)VVR_ 595.83-2.28 b Pre-immu After-immu Flag- WT K81R WT K81R / Flag
Supplementary Figure 1.TRIM33 binds β-catenin in the nucleus. a & b, Co-IP of endogenous TRIM33 with β-catenin in HT-29 cells (a) and HEK 293T cells
Supplementary Figure 1.TRIM33 binds β-catenin in the nucleus. a & b, Co-IP of endogenous TRIM33 with β-catenin in HT-29 cells (a) and HEK 293T cells (b). TRIM33 was immunoprecipitated, and the amount of
XIAP inhibits autophagy via XIAP-Mdm2-p53 signalling
The EMBO Journal (2013) 32, 2204 2216 www.embojournal.org XIAP inhibits autophagy via XIAP-Mdm2-p53 signalling THE EMBO JOURNAL Xing Huang 1,3, Zhengsheng Wu 2,3, Yide Mei 1, * and Mian Wu 1, * 1 Hefei
S1a S1b S1c. S1d. S1f S1g S1h SUPPLEMENTARY FIGURE 1. - si sc Il17rd Il17ra bp. rig/s IL-17RD (ng) -100 IL-17RD
SUPPLEMENTARY FIGURE 1 0 20 50 80 100 IL-17RD (ng) S1a S1b S1c IL-17RD β-actin kda S1d - si sc Il17rd Il17ra rig/s15-574 - 458-361 bp S1f S1g S1h S1i S1j Supplementary Figure 1. Knockdown of IL-17RD enhances
SUPPLEMENTARY INFORMATION
DOI:.38/ncb2822 a MTC02 FAO cells EEA1 b +/+ MEFs /DAPI -/- MEFs /DAPI -/- MEFs //DAPI c HEK 293 cells WCE N M C P AKT TBC1D7 Lamin A/C EEA1 VDAC d HeLa cells WCE N M C P AKT Lamin A/C EEA1 VDAC Figure
Supporting Information. FADD regulates NF-кB activation and promotes ubiquitination of cflip L to induce. apoptosis
1 2 Supporting Information 3 4 5 FADD regulates NF-кB activation and promotes ubiquitination of cflip L to induce apoptosis 6 7 Kishu Ranjan and Chandramani Pathak* 8 9 Department of Cell Biology, School
SUPPLEMENTARY FIGURES
SUPPLEMENTARY FIGURES Supplementary Figure 1. (A) Left, western blot analysis of ISGylated proteins in Jurkat T cells treated with 1000U ml -1 IFN for 16h (IFN) or left untreated (CONT); right, western
(a) Schematic diagram of the FS mutation of UVRAG in exon 8 containing the highly instable
Supplementary Figure 1. Frameshift (FS) mutation in UVRAG. (a) Schematic diagram of the FS mutation of UVRAG in exon 8 containing the highly instable A 10 DNA repeat, generating a premature stop codon
condition. Left panel, the HCT-116 cells were lysed with RIPA buffer containing 0.1%
FIGURE LEGENDS Supplementary Fig 1 (A) sumoylation pattern detected under denaturing condition. Left panel, the HCT-116 cells were lysed with RIPA buffer containing 0.1% SDS in the presence and absence
T H E J O U R N A L O F C E L L B I O L O G Y
Supplemental material Chairoungdua et al., http://www.jcb.org/cgi/content/full/jcb.201002049/dc1 T H E J O U R N A L O F C E L L B I O L O G Y Figure S1. Expression of CD9 and CD82 inhibits Wnt/ -catenin
Supplements. Figure S1. B Phalloidin Alexa488
Supplements A, DMSO, PP2, PP3 Crk-myc Figure S1. (A) Src kinase activity is necessary for recruitment of Crk to Nephrin cytoplasmic domain. Human podocytes expressing /7-NephrinCD () were treated with
GFP-LC3 +/+ CLU -/- kda CLU GFP. Actin. GFP-LC3 +/+ CLU -/- kda CLU GFP. Actin
Supplementary Fig. 1 a CQ treatment ScrB OGX11 MG132 I II AZD5363 I II b GFP / / GFP / / GFP / / GFP / / GFP GFP Actin Actin ctrl CQ GFP / / GFP / / GFP / / GFP / / GFP GFP Actin Actin rapamycin rapamycincq
Supplementary Fig. 1. GPRC5A post-transcriptionally down-regulates EGFR expression. (a) Plot of the changes in steady state mrna levels versus
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Supplementary Figure 1. PD-L1 is glycosylated in cancer cells. (a) Western blot analysis of PD-L1 in breast cancer cells. (b) Western blot analysis
Supplementary Figure 1. PD-L1 is glycosylated in cancer cells. (a) Western blot analysis of PD-L1 in breast cancer cells. (b) Western blot analysis of PD-L1 in ovarian cancer cells. (c) Western blot analysis
SUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION doi:1.138/nature9814 a A SHARPIN FL B SHARPIN ΔNZF C SHARPIN T38L, F39V b His-SHARPIN FL -1xUb -2xUb -4xUb α-his c Linear 4xUb -SHARPIN FL -SHARPIN TF_LV -SHARPINΔNZF -SHARPIN
(A) SW480, DLD1, RKO and HCT116 cells were treated with DMSO or XAV939 (5 µm)
Supplementary Figure Legends Figure S1. Tankyrase inhibition suppresses cell proliferation in an axin/β-catenin independent manner. (A) SW480, DLD1, RKO and HCT116 cells were treated with DMSO or XAV939
Rescue of mutant rhodopsin traffic by metformin-induced AMPK activation accelerates photoreceptor degeneration Athanasiou et al
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doi:10.1038/nature12652 Supplementary Figure 1. PRDM16 interacts with endogenous EHMT1 in brown adipocytes. Immunoprecipitation of PRDM16 complex by flag antibody (M2) followed by Western blot analysis
SUPPLEMENTARY INFORMATION
Figure S1 Induction of non-apoptotic death of SV40-transformed and primary DKO MEFs, and DKO thymocytes. (A-F) STS-induced non-apoptotic death of DKO MEF. (A, B) Reduced viability of DKO MEFs after exposure
SUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb2566 Figure S1 CDKL5 protein expression pattern and localization in mouse brain. (a) Multiple-tissue western blot from a postnatal day (P) 21 mouse probed with an antibody against CDKL5.
SUPPLEMENTARY FIGURES AND TABLE
SUPPLEMENTARY FIGURES AND TABLE Supplementary Figure S1: Characterization of IRE1α mutants. A. U87-LUC cells were transduced with the lentiviral vector containing the GFP sequence (U87-LUC Tet-ON GFP).
A. List of selected proteins with high SILAC (H/L) ratios identified in mass
Supplementary material Figure S1. Interaction between UBL5 and FANCI A. List of selected proteins with high SILAC (H/L) ratios identified in mass spectrometry (MS)-based analysis of UBL5-interacting proteins,
Supplementary Figure 1
Supplementary Figure 1 a γ-h2ax MDC1 RNF8 FK2 BRCA1 U2OS Cells sgrna-1 ** 60 sgrna 40 20 0 % positive Cells (>5 foci per cell) b ** 80 sgrna sgrna γ-h2ax MDC1 γ-h2ax RNF8 FK2 MDC1 BRCA1 RNF8 FK2 BRCA1
Supplemental Materials. STK16 regulates actin dynamics to control Golgi organization and cell cycle
Supplemental Materials STK16 regulates actin dynamics to control Golgi organization and cell cycle Juanjuan Liu 1,2,3, Xingxing Yang 1,3, Binhua Li 1, Junjun Wang 1,2, Wenchao Wang 1, Jing Liu 1, Qingsong
Supplement Figure S1. Real Time PCR analysis of mrna levels of C/EBPα and PU.1 in wild type (WT) and NQO1-null (NQO1-/-) mice.
competes with 20S proteasome for binding with C/EBP leading to its stabilization and Relative mrna levels Supplement Figure S1. Real Time PCR analysis of mrna levels of C/EBPα and PU.1 in wild type (WT)
Supplementary Figures
Supplementary Figures Supplementary Figure 1 DOT1L regulates the expression of epithelial and mesenchymal markers. (a) The expression levels and cellular localizations of EMT markers were confirmed by
293T cells were transfected with indicated expression vectors and the whole-cell extracts were subjected
SUPPLEMENTARY INFORMATION Supplementary Figure 1. Formation of a complex between Slo1 and CRL4A CRBN E3 ligase. (a) HEK 293T cells were transfected with indicated expression vectors and the whole-cell
Supplementary Table 1. List of primers used in this study
Supplementary Table 1. List of primers used in this study Gene Forward primer Reverse primer Rat Met 5 -aggtcgcttcatgcaggt-3 5 -tccggagacacaggatgg-3 Rat Runx1 5 -cctccttgaaccactccact-3 5 -ctggatctgcctggcatc-3
Supplementary Table 1. Metabolic parameters in GFP and OGT-treated mice
Supplementary Table 1. Metabolic parameters in GFP and OGT-treated mice Fasted Refed GFP OGT GFP OGT Liver G6P (mmol/g) 0.03±0.01 0.04±0.02 0.60±0.04 0.42±0.10 A TGs (mg/g of liver) 20.08±5.17 16.29±0.8
Supplementary Materials
Supplementary Materials Supplementary Figure S1 Regulation of Ubl4A stability by its assembly partner A, The translation rate of Ubl4A is not affected in the absence of Bag6. Control, Bag6 and Ubl4A CRISPR
Supplementary Figure 1. mir124 does not change neuron morphology and synaptic
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Supplementary Figure S1: Defective heterochromatin repair in HGPS progeroid cells
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Supplementary Fig. S1. Schematic diagram of minigenome segments.
open reading frame 1565 (segment 5) 47 (-) 3 5 (+) 76 101 125 149 173 197 221 246 287 open reading frame 890 (segment 8) 60 (-) 3 5 (+) 172 Supplementary Fig. S1. Schematic diagram of minigenome segments.
Nature Structural and Molecular Biology: doi: /nsmb Supplementary Figure 1
Supplementary Figure 1 Mutational analysis of the SA2-Scc1 interaction in vitro and in human cells. (a) Autoradiograph (top) and Coomassie stained gel (bottom) of 35 S-labeled Myc-SA2 proteins (input)
Supplementary Figure 1: si-craf but not si-braf sensitizes tumor cells to radiation.
Supplementary Figure 1: si-craf but not si-braf sensitizes tumor cells to radiation. (a) Embryonic fibroblasts isolated from wildtype (WT), BRAF -/-, or CRAF -/- mice were irradiated (6 Gy) and DNA damage
Tel: ; Fax: ;
Tel.: +98 216 696 9291; Fax: +98 216 696 9291; E-mail: mrasadeghi@pasteur.ac.ir Tel: +98 916 113 7679; Fax: +98 613 333 6380; E-mail: abakhshi_e@ajums.ac.ir A Soluble Chromatin-bound MOI 0 1 5 0 1 5 HDAC2
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Supplementary Figure 1. PAQR3 knockdown inhibits SREBP-2 processing in CHO-7 cells CHO-7 cells were transfected with control sirna or a sirna
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Nature Immunology: doi: /ni.3866
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Supplementary Figure 1. Normal T lymphocyte populations in Dapk -/- mice. (a) Normal thymic development in Dapk -/- mice. Thymocytes from WT and Dapk
Supplementary Figure 1. Normal T lymphocyte populations in Dapk -/- mice. (a) Normal thymic development in Dapk -/- mice. Thymocytes from WT and Dapk -/- mice were stained for expression of CD4 and CD8.
SUPPLEMENTARY INFORMATION
Supplementary Table 1. Cell sphingolipids and S1P bound to endogenous TRAF2. Sphingolipid Cell pmol/mg TRAF2 immunoprecipitate pmol/mg Sphingomyelin 4200 ± 250 Not detected Monohexosylceramide 311 ± 18
hexahistidine tagged GRP78 devoid of the KDEL motif (GRP78-His) on SDS-PAGE. This
SUPPLEMENTAL FIGURE LEGEND Fig. S1. Generation and characterization of. (A) Coomassie staining of soluble hexahistidine tagged GRP78 devoid of the KDEL motif (GRP78-His) on SDS-PAGE. This protein was expressed
Supplemental Information. NRF2 Is a Major Target of ARF. in p53-independent Tumor Suppression
Molecular Cell, Volume 68 Supplemental Information NRF2 Is a Major Target of ARF in p53-independent Tumor Suppression Delin Chen, Omid Tavana, Bo Chu, Luke Erber, Yue Chen, Richard Baer, and Wei Gu Figure
Supplementary Figures
Supplementary Figures Supplementary Figure 1 Characterization of stable expression of GlucB and sshbira in the CT26 cell line (a) Live cell imaging of stable CT26 cells expressing green fluorescent protein
Supplementary Figure 1
Supplementary Figure 1 YAP negatively regulates IFN- signaling. (a) Immunoblot analysis of Yap knockdown efficiency with sh-yap (#1 to #4 independent constructs) in Raw264.7 cells. (b) IFN- -Luc and PRDs
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DOI: 10.1038/ncb2607 Figure S1 Elf5 loss promotes EMT in mammary epithelium while Elf5 overexpression inhibits TGFβ induced EMT. (a, c) Different confocal slices through the Z stack image. (b, d) 3D rendering
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doi:10.1038/nature11700 Figure 1: RIP3 as a potential Sirt2 interacting protein. Transfected Flag-tagged Sirt2 was immunoprecipitated from cells and eluted from the Sepharose beads using Flag peptide.
Supplemental Figure 1. Western blot analysis indicated that MIF was detected in the fractions of
Supplemental Figure Legends Supplemental Figure 1. Western blot analysis indicated that was detected in the fractions of plasma membrane and cytosol but not in nuclear fraction isolated from Pkd1 null
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FIG S1 Examination of eif4b expression after virus infection. (A) A549 cells
Supplementary Figure Legends FIG S1 Examination of expression after virus infection. () 549 cells were infected with herpes simplex virus (HSV) (MOI = 1), and harvested at the indicated times, followed
Title of file for HTML: Supplementary Information Description: Supplementary Figures and Supplementary Table
Title of file for HTML: Supplementary Information Description: Supplementary Figures and Supplementary Table Title of file for HTML: Peer Review File Description: Innate Scavenger Receptor-A regulates
(a) Significant biological processes (upper panel) and disease biomarkers (lower panel)
Supplementary Figure 1. Functional enrichment analyses of secretomic proteins. (a) Significant biological processes (upper panel) and disease biomarkers (lower panel) 2 involved by hrab37-mediated secretory
SUPPLEMENTARY INFORMATION. Supplementary Figures S1-S9. Supplementary Methods
SUPPLEMENTARY INFORMATION SUMO1 modification of PTEN regulates tumorigenesis by controlling its association with the plasma membrane Jian Huang 1,2#, Jie Yan 1,2#, Jian Zhang 3#, Shiguo Zhu 1, Yanli Wang
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Supplementary Information mediates STAT3 activation at retromer-positive structures to promote colitis and colitis-associated carcinogenesis Zhang et al. a b d e g h Rel. Luc. Act. Rel. mrna Rel. mrna
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File Name: Supplementary Information Description: Supplementary Figures and Supplementary Tables File Name: Peer Review File Description: Primer Name Sequence (5'-3') AT ( C) RT-PCR USP21 F 5'-TTCCCATGGCTCCTTCCACATGAT-3'
Tbk1-TKO! DN cells (%)! 15! 10!
a! T Cells! TKO! B Cells! TKO! b! CD4! 8.9 85.2 3.4 2.88 CD8! Tbk1-TKO! 1.1 84.8 2.51 2.54 c! DN cells (%)! 4 3 2 1 DP cells (%)! 9 8 7 6 CD4 + SP cells (%)! 5 4 3 2 1 5 TKO! TKO! TKO! TKO! 15 1 5 CD8
Supplemental Material:
Supplemental Material: MATERIALS AND METHODS RNA interference Mouse CHOP sirna (ON-TARGETplus SMARTpool Cat# L-062068-00) and control sirna (ON-TARGETplus Control) were purchased from Dharmacon. Transfection
Supplementary Figure 1
Supplementary Figure 1 Supplementary Figure 1 Schematic depiction of the tandem Fc GDF15. Supplementary Figure 2 Supplementary Figure 2 Gfral mrna levels in the brains of both wild-type and knockout Gfral
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SUPPLEMENTARY FIGURE LEGENDS
SUPPLEMENTARY FIGURE LEGENDS Supplemental FIG. 1. Localization of myosin Vb in cultured neurons varies with maturation stage. A and B, localization of myosin Vb in cultured hippocampal neurons. A, in DIV
genome edited transient transfection, CMV promoter
Supplementary Figure 1. In the absence of new protein translation, overexpressed caveolin-1-gfp is degraded faster than caveolin-1-gfp expressed from the endogenous caveolin 1 locus % loss of total caveolin-1-gfp
Supplementary Materials for
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Supplementary Figure 1. HOPX is hypermethylated in NPC. (a) Methylation levels of HOPX in Normal (n = 24) and NPC (n = 24) tissues from the
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Nature Immunology doi: /ni.3268
Supplementary Figure 1 Loss of Mst1 and Mst2 increases susceptibility to bacterial sepsis. (a) H&E staining of colon and kidney sections from wild type and Mst1 -/- Mst2 fl/fl Vav-Cre mice. Scale bar,
SUPPLEMENTAL MATERIALS AND METHODS. Puromycin-synchronized metabolic labelling - Transfected HepG2 cells were depleted of
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doi:10.1038/nature11095 Supplementary Table 1. Summary of the binding between Angptls and various Igdomain containing receptors as determined by flow cytometry analysis. The results were summarized from
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DOI: 10.1038/ncb2988 Supplementary Figure 1 Kif7 L130P encodes a stable protein that does not localize to cilia tips. (a) Immunoblot with KIF7 antibody in cell lysates of wild-type, Kif7 L130P and Kif7
Supplementary Figure 1: Co-localization of reconstituted L-PTC and dendritic cells
a CD11c Na + K + ATPase Na + K + ATPase CD11c x-y CD11c Na + K + ATPase Na + K + ATPase CD11c x-z c b x-y view BoNT NAPs CD11c BoNT CD11c NAPs BoNT NAPs CD11c 90 x-z view Apical Basolateral Supplementary
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supplementary information
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a 1% 5% % 1% 5% % OVAb βgal activity A.U. (x1 4 ) 2 1 5 βgal activity A.U. (x1 4 ) 2 1 BSAb 2 hours 4 hours OVAb BSAb OVAb BSAb,1 1 1 1 SIINFEKL (ng/ml) CFSE b Beads Alexa488 (%) 8 6 4 2 ** ** 1:1 5:1
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TERT or IgG as indicated. For the western blots, representative images of n= independent experiments are shown. Student s t-test was used, and * indicates p
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Supplementary Figure 1 Supplementary Figure 1. Neither the activation nor suppression of the MAPK pathway affects the ASK1/Vif interaction. (a, b) HEK293 cells were cotransfected with plasmids encoding
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