Clinical significance and prognostic value of receptor conversion in hormone receptor positive breast cancers after neoadjuvant chemotherapy

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1 Yang et al. World Journal of Surgical Oncology (2018) 16:51 RESEARCH Open Access Clinical significance and prognostic value of receptor conversion in hormone receptor positive breast cancers after neoadjuvant chemotherapy Libo Yang 1,3, Xiaorong Zhong 2, Tianjie Pu 1,3, Yan Qiu 1,3, Feng Ye 3 and Hong Bu 1,3* Abstract Background: Neoadjuvant chemotherapy (NAC) is widely used in advanced breast cancer patients. However, there is little known about conversion frequency of estrogen receptor (ER) and/or progesterone receptor (PR) status for hormone receptor positive-breast cancer patients after NAC and their correlation with prognosis. Methods: In this study, 231 breast cancer patients with residual disease after NAC were enrolled and divided into receptor stable group (having no conversion in both ER and PR status pre- and post-nac) and any receptor conversion group (having any conversion in either ER or PR status). Univariate and multivariate survival analyses were used to compare survival differences between the two groups. Results: Fifty-five patients (23.8%) had ER and/or PR conversion after NAC. Younger patients ( 50 years) were more likely to have receptor conversion (P = 0.014). For 213 patients (92.2%) who received adjuvant endocrinotherapy after surgery, the 5-year disease free survival (DFS) estimates for patients in the any receptor conversion group (55. 2%) was worse than patients in the receptor stable group (73.7%, Log-rank test, P = 0.015). While the 5-year overall survival estimates for patients with or without receptor conversion were not statistically different (86.0 vs. 82.4%, Log-rank test, P = 0.587). In multivariate Cox proportional hazard analyses, patients with any receptor conversion had worse DFS (hazard ratio, 1.995; 95% confidence interval, , P = 0.031). Conclusions: It is necessary to recommend patients to test biomarkers in residual disease and pay more attention to patients who have any receptor conversion. These patients may need more individual therapy after surgery. Keywords: Breast cancer, Neoadjuvant chemotherapy, Hormone receptor, Receptor conversion Background Neoadjuvant chemotherapy (NAC) has been generally accepted in patients with locally advanced breast cancer. Besides reducing tumor stage, NAC is a practical approach to individually test chemosensitivity preoperatively. And some patients who reached pathologic complete response (pcr) through NAC tend to have prefer prognosis compared with those who still have residual disease [1 4]. Guarneri et al. [2] reported that * Correspondence: hbpathology@126.com 1 Department of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China 3 Laboratory of Pathology, West China Hospital, Sichuan University, Guo Xue Xiang No.37, Chengdu, Sichuan Province , China Full list of author information is available at the end of the article 5-year disease-free survival rates were 87.1 vs. 61.1% and 10-year disease-free survival rates were 74.9 vs. 39.9% for patients with and without pcr, respectively. Before treatment, core needle biopsy (CNB) is essential for diagnosis and biomarkers assessment. Hormone (estrogen and progesterone) receptors (HR) are important biomarkers in breast cancers and indication of adjuvant endocrinotherapy [5 7]. However, patients with HR expression tumors benefit less from chemotherapy since they have lower pcr rates compared with patients with HR negative [8 10]. Conversely, HR expression is a predictor of favorable prognosis in breast cancers partially because of adjuvant endocrinotherapy. In general, HR The Author(s) Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

2 Yang et al. World Journal of Surgical Oncology (2018) 16:51 Page 2 of 9 status has an unreplaceable predictive and prognostic value for breast cancer patients. Immunohistochemistry (IHC) is a standard method to quantify HR expression mainly based on the fraction of stained tumor cells. Several studies have reported conversions of estrogen receptor (ER) and progesterone receptor (PR) status between CNB and surgical specimens after NAC [11 13]. Conversions of receptor are also prevalent in patients that did not receive NAC, it is statistically more frequent in NAC patients [14]. However, the influences of these conversions on following treatment and prognosis in breast cancers are still unclear. The objectives of this study were to assess the conversion frequency of ER and/or PR status in HR expression patients after NAC and to determine whether these conversions influence prognosis. Methods Patients and samples This study was approved by the institutional Ethics Committee of the West China Hospital. We retrospectively collected the clinicopathological data of 256 breast cancer patients with HR expression (ER and/or PR positive) in pre-nac specimens between January 2009 and December All those patients were in clinical tumor stage II to III with a follow-up time more than 6 months. In this study, the regimen of NAC was based on anthracycline and/or taxane. No patient received adjuvant anti-her2- targeted therapy or endocrine therapy before surgery. Before NAC, patients underwent ultrasound-guided needle biopsy for histological examination. Pathologists at West China Hospital reconfirmed hematoxylin and eosin (H&E) slides of specimens and evaluated residual tumor cells at primary tumor site (breast) of surgical specimens. Histological type was also recorded for analyses. Therefore, 25 patients were excluded from this study because of no or few tumor cells in primary tumor site. Finally, 231 formalin-fixed paraffin-embedded (FFPE) pre-nac and matched surgical specimens were included in this study (Fig. 1). Immunohistochemistry Biomarker status were evaluated using pre-nac and surgical FFPE tissue blocks by IHC. IHC staining of tumor tissues for ER (Confirm anti-er (SP1), rabbit monoclonal antibody, Ventana Medical Systems), PR (Confirm anti-pr (1E2), rabbit monoclonal antibody, Ventana Medical Systems), human epidermal growth factor receptor 2 (HER2) (Ventana anti-her2/neu (4B5), rabbit monoclonal antibody, Ventana Medical Systems), and Ki67 (Confirm anti-ki67 (30 9), rabbit monoclonal antibody, Ventana Medical Systems) was performed using the automated Benchmark XT platform (Ventana Medical Systems) and according to the manufacturer s recommendations. The cutoff value of ER- and PR-positive disease was no less than 1% positively stained nuclei in tumor tissues. HER2-positive status was defined as 3(+) by IHC or amplification confirmed by fluorescence in situ hybridization (FISH). Ki67 expression was divided into high expression group ( 15%) and low expression group (< 15%). Fig. 1 Flowchart showing the inclusion of this study. (HR, hormone receptor; pcr, pathologic complete response)

3 Yang et al. World Journal of Surgical Oncology (2018) 16:51 Page 3 of 9 Statistical analysis In this study, patients with residual disease at primary tumor site after NAC were classified as receptor stable group (having no conversion in both ER and PR status) and any receptor conversion group (having any conversion in either ER or PR status). And in this study, receptor conversion meant receptor status changed from positive to negative or from negative to positive after NAC. Pearson χ 2 test was used to compare pre-nac clinicopathological differences between the two groups. Patient prognosis was evaluated through disease-free survival (DFS) and overall survival (OS). DFS was defined as the interval from the date of surgery to the detection of relapse, death from any cause, or the date of the last visit for patients without events. OS was defined as the interval from the date of surgery to death or the date of the last visit for patients without events. Survival analyses were performed using Kaplan-Meier method for DFS and OS. The survival curves were compared using Log-rank test. Univariate and multivariate Cox proportional hazard analyses were used to determine the association of clinicopathological factors with DFS and OS. The two-sided significance level was set at P < In addition, multivariate Cox proportional hazard analyses only included variables that were statistically significant (P < 0.05) in univariate analyses. Results Patient characteristics Twenty-two of 256 HR positive patients had no residual tumor cell at primary tumor site and 13 patients reached pcr through NAC. The pcr rate in those patients was 5.08%. Three patients that had few residual tumor cells at the primary tumor site that were inadequate for biomarker assessment were also excluded from study. For the remaining 231 patients, 158 patients (68.4%) were under 50 years old, and the mean age of all patients was ± 8.93 years. About tumor histological type, 201 patients (87.0%) had invasive ductal carcinoma, 17 patients (7.4%) had invasive ductal carcinoma mixed with other invasive carcinoma, and 13 patients (5.6%) had other invasive carcinoma, such as invasive lobular carcinoma (7 patients), mucinous adenocarcinoma (5 patients), and invasive cribriform carcinoma (1 patients). Before receiving NAC, 41 patients (17.7%) had tumor invading chest wall and 199 patients (86.1%) had lymph node involvement. Though all included tumors were HR positive (ER and/or PR positive), 221 tumors (95.7%) were ER positive and 200 tumors (86.6%) were PR positive. HER2 status was positive in 37 patients (16.0%), and Ki67 expression was high in 172 patients (74.5%). All patients underwent one to eight of NAC using anthracycline-based regimens (30.7%), anthracycline- and taxane-based regimens (60.6%), and taxanebased regimens (8.7%). Table 1 Patients characteristics according to receptor stable and any receptor conversion Characteristics All patients Receptor stable Any receptor conversion (N = 231) (N = 176) (N = 55) Age, years (68.4) 113 (64.2) 45 (81.8) > (31.6) 63 (35.8) 10 (18.2) Initial tumor stage T1 41 (17.7) 34 (19.3) 7 (12.7) T2 119 (51.5) 90 (51.1) 29 (52.7) T3 or T4 71 (30.7) 52 (29.5) 19 (34.5) Initial node status Negative 32 (13.9) 22 (12.5) 10 (18.2) Positive 199 (86.1) 154 (87.5) 45 (81.8) Nuclear grade 1 or 2 74 (32.0) 60 (56.6) 14 (46.7) (26.8) 46 (43.4) 16 (53.3) NA 95 (41.1) Histology IDC 201 (87.0) 154 (87.5) 47 (85.5) Mixed 17 (7.4) 13 (7.4) 4 (7.3) Other 13 (5.6) 9 (5.1) 4 (7.3) Initial ER status Negative 10 (4.3) 3 (1.7) 7 (12.7) < Positive 221 (95.7) 173 (98.3) 48 (87.3) Initial PR status Negative 31 (13.4) 18 (10.2) 13 (23.6) Positive 200 (86.6) 158 (89.8) 42 (76.4) Initial HER2 status Negative 194 (84.0) 150 (85.2) 44 (80.0) Positive 37 (16.0) 26 (14.8) 11 (20.0) Initial Ki-67 status < 15% 59 (25.5) 48 (27.3) 11 (20.0) % 172 (74.5) 128 (72.7) 44 (80.0) NAC cycles (66.2) 117 (66.5) 36 (65.5) (33.8) 59 (33.5) 19 (34.5) NAC regimens Anthracycline-based 71 (30.7) 53 (30.1) 18 (32.7) Anthracycline- and 140 (60.6) 107 (60.8) 33 (60.0) taxane-based Taxane-based 20 (8.7) 16 (9.1) 4 (7.3) ER estrogen receptor, PR progesterone receptor, HER2 human epidermal growth factor receptor 2, NAC neoadjuvant chemotherapy P

4 Yang et al. World Journal of Surgical Oncology (2018) 16:51 Page 4 of 9 Fig. 2 Percentage of ER and PR expression pre- and post-nac for patients with any receptor conversion. a Percentage of ER expression pre- and post-nac for patients with any receptor conversion. b Percentage of PR expression pre- and post-nac for patients with any receptor conversion. (ER estrogen receptor, PR progesterone, NAC neoadjuvant chemotherapy) Conversion in receptor status Patients characteristics before NAC according to receptor conversion are listed in Table 1. Fifty-five patients (23.8%) had ER and/or PR conversion after NAC. The percentage of ER and PR expression preand post-nac was shown in Fig. 2. ER conversion happened in 13 patients (5.6%), which contained positive-to-negative conversion in 9 patients and negative-to-positive conversion in 4 patients. Meanwhile, PR conversion happened in 45 patients (19.5%), which contained positive-to-negative conversion in 37 patients and negative-to-positive conversion in 8 patients. Moreover, 11 patients (4.8%) had HR negative (ER and PR negative) disease after NAC. We found younger ( 50 years) patients tended to convert in receptor status more frequently (P =0.014). However, there were no significant differences in tumor stage, node status, nuclear grade, histology, NAC regimens or cycles, HER2 status, and Ki-67 status between the receptor stable group and the any receptor conversion group. Follow-up and survival analysis Adjuvant endocrinotherapy was given to 167 (94.9%) of 176 patients in the receptor stable group and 46 (83.6%) of 55 patients in the any receptor conversion group. For patients who had HR negative disease after NAC, there were 45.5% (5 of 11) of patients who still received adjuvant endocrinotherapy depending on personal and/or oncologist preferences. In total, there were 18 patients who did not receive adjuvant endocrinotherapy after surgery. And those patients were not included into the next survival analyses. During the follow-up time (range, 7 87 months), 24 (11.3%) of 213 patients had died, and 54 (25.4%) of 213 patients had experienced disease recurrence. The 5-year DFS estimates for patients in the receptor stable group (73.7%) were significantly higher than patients in the any receptor conversion group (55.2%, P = 0.015, Fig. 3a); however, the 5-year OS estimates between patients in the receptor stable group (86.0%) and patients in the any receptor conversion group (82.4%) were not significantly different (P = 0.587, Fig. 3b). Fig. 3 Survival estimates according to receptor conversion status. a Disease-free survival estimates (Log-rank test, P = 0.015). b Overall survival estimates (Log-rank test, P = 0.587)

5 Yang et al. World Journal of Surgical Oncology (2018) 16:51 Page 5 of 9 Table 2 Univariate and multivariate analyses of disease-free survival for initial HR-positive patients with adjuvant endocrinotherapy after surgery Characteristics N (%) DFS Uv Mv P HR (95% CI) P HR (95% CI) Age, years (68.5) 1.00 > (31.5) ( ) Initial tumor stage T1 38 (17.8) 1.00 T2 107 (50.2) ( ) T3 or T4 68 (31.9) ( ) Initial node status Negative 26 (12.2) Positive 187 (87.8) ( ) ( ) Nuclear grade or 2 73 (34.3) (25.8) ( ) NA 85 (39.9) Histology IDC 183 (85.9) 1.00 Mixed 17 (8.0) ( ) Other 13 (6.1) ( ) Initial HER2 status Negative 182 (85.4) 1.00 Positive 31 (14.5) ( ) Initial Ki-67 status < 15% 57 (26.8) % 156 (73.2) ( ) NAC regimens Anthracycline-based 65 (30.5) 1.00 Anthracycline- and Taxane-based 133 (62.4) ( ) Taxane-based 15 (7.0) ( ) Any receptor conversion No 167 (78.4) Yes 46 (21.6) ( ) ( ) Tumor size at surgery cm 88 (41.3) 1.00 > 2 and 5 cm 114 (53.5) ( ) > 5 cm 11 (5.2) ( ) Node status at surgery Negative 49 (23.0) Positive 164 (77.0) ( ) ( ) HER2 status at surgery Negative 184 (86.4) 1.00 Positive 29 (13.6) ( )

6 Yang et al. World Journal of Surgical Oncology (2018) 16:51 Page 6 of 9 Table 2 Univariate and multivariate analyses of disease-free survival for initial HR-positive patients with adjuvant endocrinotherapy after surgery (Continued) Characteristics N (%) DFS Uv Mv P HR (95% CI) P HR (95% CI) Ki-67 status at surgery < 15% 116 (54.5) % 97 (45.5) ( ) Radiotherapy No 57 (26.8) Yes 156 (73.2) ( ) ( ) DFS disease-free survival, HR hazard ratio, CI confidence interval, Uv univariate Cox proportional hazard analyses, Mv multivariate Cox proportional hazard analyses, IDC invasive ductal carcinoma, HER2 human epidermal growth factor receptor 2, NAC neoadjuvant chemotherapy The univariate and multivariate Cox regression analyses of DFS and OS about pre- and post-nac characteristics in 213 patients who received adjuvant endocrinotherapy are listed in Tables 2 and 3. In this study, patients were divided into the receptor stable group and the no receptor conversion group according to pre- and post-nac ER and PR status. Therefore, pre- and post- NAC ER and PR status were not included in the survival analyses to avoid interaction effect with patient grouping. Moreover, 7 (22.6%) of 31 tumors had positive-to-negative change, and 5 (2.7%) of 182 tumors had negative-topositive change in HER2 status for patients included in the survival analyses. So, both pre- and post-nac HER2 status were considered in the analyses. The multivariate Cox proportional hazard analyses only included variables that were statistically significant (P < 0.05) in univariate analyses. Any receptor conversion status was a significant predictor of DFS (hazard ratio (HR), 1.995; 95% confidence interval (CI), ; P =0.031). Discussion This study focused on ER and/or PR conversion after receiving NAC and its influences on the prognosis and adjuvant endocrinotherapy in breast cancer patients. Only HR positive tumors are recommended to receive adjuvant endocrinotherapy. There are no clear guidelines about adjuvant endocrinotherapy for those patients who had ER and/or PR conversion after NAC. Therefore, only HR-positive disease patients before NAC were enrolled to reduce the influence of adjuvant endocrinotherapy after surgery. In addition, patients with HR negative disease were more sensitive to chemotherapy [15, 16], and less patients had residual disease at breast after NAC to assess biomarkers. In this retrospective study, we set positive ER and PR expression cutoff value as 1%. For 231 HRpositive disease before NAC, 55 (23.8%) had ER and/ or PR conversion, 13 (5.6%) had ER conversion, and 45 (19.5%) had PR conversion. Hirata et al. [12] used 10% as the cutoff value for ER and PR expression. Of 214 HR positive disease in their study, 38 (17.8%) had ER conversion and 89 (41.6%) had PR conversion after NAC. Other studies which used a cutoff of 5% or Allred score ( 3) to define ER and PR positive staining also reported discordance of receptor expression after NAC [11, 17]. Except for sampling error of core needle biopsy before treatment, receiving NAC may be a main cause of this phenomenon [14]. Our analysis show that younger ( 50 years) patients were more likely to have conversion, due to an unknown underlying mechanism. The influence of HR status changes to prognosis remained controversial. Hirata et al. [12] didnotfind a significant difference in DFS and OS between the HR-positive group and HR-conversion group. However, in their study, there were 18 (38.3%) of 47 patients who had HR negative tumor before NAC in the HR conversion group. Chen et al. [18] adjusted the influence of adjuvant endocrinotherapy in HR positive patients and found negative influence in DFS and OS for patients who had HR positive to negative change after NAC. Parinyanitikul et al. [19] used the 20% cutoff for absolute percent change for ER and PR expression and found that patients with any receptor change had better relapse-free survival (RFS) than patients with no receptor change. Those previous studies focused on the influence of HR status conversion or absolute percent change of ER or PR expression, but there were few studies focused on the influences of at least one of ER and PR status conversion in HR positive patients. After adjusting the influence of adjuvant endocrinotherapy, patients with receptor stable disease had significantly higher DFS than patients with any receptor conversion (P = 0.017). Our

7 Yang et al. World Journal of Surgical Oncology (2018) 16:51 Page 7 of 9 Table 3 Univariate and multivariate analyses of overall survival for initial HR-positive patients with adjuvant endocrinotherapy after surgery Characteristics N (%) OS Uv Mv P HR (95% CI) P HR (95% CI) Age, years (68.5) 1.00 > (31.5) ( ) Initial tumor stage T1 38 (17.8) 1.00 T2 107 (50.2) ( ) T3 or T4 68 (31.9) ( ) Initial node status Negative 26 (12.2) 1.00 Positive 187 (87.8) ( ) Nuclear grade or 2 73 (34.3) (25.8) ( ) NA 85 (39.9) Histology IDC 183 (85.9) Mixed 17 (8.0) ( ) ( ) Other 13 (6.1) ( ) ( ) Initial HER2 status Negative 182 (85.4) 1.00 Positive 31 (14.5) ( ) Initial Ki-67 status < 15% 57 (26.8) % 156 (73.2) ( ) NAC regimens Anthracycline-based 65 (30.5) Anthracycline- and Taxane-based 133 (62.4) ( ) ( ) Taxane-based 15 (7.0) ( ) ( ) Any receptor conversion No 167 (78.4) 1.00 Yes 46 (21.6) ( ) Tumor size at surgery cm 88 (41.3) 1.00 > 2 and 5 cm 114 (53.5) ( ) > 5 cm 11 (5.2) ( ) Node status at surgery Negative 49 (23.0) 1.00 Positive 164 (77.0) ( ) HER2 status at surgery Negative 184 (86.4) 1.00

8 Yang et al. World Journal of Surgical Oncology (2018) 16:51 Page 8 of 9 Table 3 Univariate and multivariate analyses of overall survival for initial HR-positive patients with adjuvant endocrinotherapy after surgery (Continued) Characteristics N (%) OS Uv Mv P HR (95% CI) P HR (95% CI) Positive 29 (13.6) ( ) Ki-67 status at surgery < 15% 116 (54.5) % 97 (45.5) ( ) Radiotherapy No 57 (26.8) 1.00 Yes 156 (73.2) ( ) OS overall survival, HR hazard ratio, CI confidence interval, Uv Univariate Cox proportional hazard analyses, Mv multivariate Cox proportional hazard analyses, IDC invasive ductal carcinoma, HER2 human epidermal growth factor receptor 2, NAC neoadjuvant chemotherapy multivariable analysis also revealed that any receptor conversion status was associated with worse DFS (HR, 1.881; 95% CI, ; P = 0.031). While 5- year OS estimates for patients without any receptor conversion was higher than the other patients (86.0 vs. 82.4%), the difference was not statistically significant in univariate analysis (P = 0.588). Moreover, we also analyzed survival differences for patients who did not receive endocrinotherapy between the two groups. For nine patients with any receptor conversion, the 5- year DFS and 5-year OS rates were 77.8 and 88.9%, respectively. And these rates were not only similar to patients with no receptor conversion (88.9 and 88.9%) but also similar to patients with any receptor conversion who received adjuvant endocrinotherapy (60.8 and 87.0%). In this study, the proportion of HER2 positive-tonegative change was higher than negative-to-positive change (22.6 vs. 2.7%). However, it only reflected change pattern of tumors which were HR-positive before NAC. Niikura et al. [20] reported change pattern of HER2 status regardless of pre-nac HR status, and they had similar results (21.4% for HER2 positive-to-negative change and 3.4% for HER2 negative-to-positive change) to ours. Though HER2 status may have conversion after receiving NAC, this phenomenon was not significant when compared with patients who did not receive NAC [14]. By the way, 52.4% of patients had low Ki- 67 expression after NAC, and it was much more than the proportion (25.5%) before NAC (P < 0.01). Above 60% of breast cancer patients with non-pcr disease had reduction of Ki-67 expression after NAC [21, 22]. Some studies had also reported that patients with reduction of Ki-67 expression had more favorable prognosis after NAC [21, 22]. In our present data, we did not do further study on the influence of reduction of Ki-67 expression on prognosis. Conclusions Our study showed that conversion in ER and/or PR status after NAC should not be neglected for breast cancer patients. Patients with any conversion in ER or PR status was associated with poor DFS after adjusting for adjuvant endocrinotherapy. Biomarkers testing is recommended in patients with residual disease for receptor conversion. These patients may need more individualized therapy after surgery. Abbreviations CNB: Core needle biopsy; DFS: Disease-free survival; ER: Estrogen receptor; HER2: Human epidermal growth factor receptor 2; HR: Hormone receptor; IHC: Immunohistochemistry; NAC: Neoadjuvant chemotherapy; OS: Overall survival; pcr: Pathologic complete response; PR: Progesterone receptor Acknowledgements Not applicable. Funding None. Availability of data and materials None. Authors contributions LBY, FY, and HB designed the experiments. XRZ did the follow-up record. LBY, YQ, and TJP analyzed the data and prepared the manuscript. All authors read and approved the final manuscript. Ethics approval and consent to participate This study was approved by the institutional Ethics Committee of the West China Hospital. Consent for publication Not applicable. Competing interests The authors declare that they have no competing interests. Publisher s Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

9 Yang et al. World Journal of Surgical Oncology (2018) 16:51 Page 9 of 9 Author details 1 Department of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China. 2 Departments of Head and Neck and Mammary Gland Oncology, and Medical Oncology, Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China. 3 Laboratory of Pathology, West China Hospital, Sichuan University, Guo Xue Xiang No.37, Chengdu, Sichuan Province , China. Received: 30 September 2017 Accepted: 4 February 2018 References 1. Rastogi P, Anderson SJ, Bear HD, et al. Preoperative chemotherapy: updates of National Surgical Adjuvant Breast and Bowel Project Protocols B-18 and B-27. J Clin Oncol. 2008;26: Guarneri V, Broglio K, Kau SW, et al. Prognostic value of pathologic complete response after primary chemotherapy in relation to hormone receptor status and other factors. J Clin Oncol. 2006;24: Kuerer HM, Newman LA, Smith TL, et al. 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